CN101824437A - Method for producing hydrogen by utilizing facultative anaerobe fermentation - Google Patents
Method for producing hydrogen by utilizing facultative anaerobe fermentation Download PDFInfo
- Publication number
- CN101824437A CN101824437A CN 201019026129 CN201019026129A CN101824437A CN 101824437 A CN101824437 A CN 101824437A CN 201019026129 CN201019026129 CN 201019026129 CN 201019026129 A CN201019026129 A CN 201019026129A CN 101824437 A CN101824437 A CN 101824437A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- hydrogen
- facultative anaerobe
- substratum
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method for producing hydrogen by utilizing facultative anaerobe fermentation, and in particular relates to two aspects of strain screening and fermentation condition optimization. The screened facultative anaerobe is Enterobacter aerogenes CICC10293; through tests, the optimal fermentation process conditions of a strain are as follows: a culture medium is concretely prepared from 4 to 6g/L of peptone, 2 to 4g/L of beef extract, 4 to 6g/L of NaCl, 1 to 1.5g/L of K2HPO4 and 0.1 to 0.2g/L of FeSO4.4H2O; a fermentation substrate is dextrose, and the concentration is 20g/l; fermentation adopts a mode of batch fermentation; constant temperature shaking culture is carried out under conditions that the pH is 6.0 to 7.0 and the temperature is 40 DEG C, and the shaking frequency is 170r/min; and the culture time is about 20 hours until hydrogen production stops. The hydrogen yield can reach 134mLH2/100mL of liquid culture medium, the hydrogen production efficiency is high, the requirement for fermentation environment conditions is low, and the invention has broad application aspect.
Description
Technical field
The present invention relates to a kind of method of biological hydrogen production, especially a kind of method of utilizing facultative anaerobe hydrogen manufacturing.
Background technology
Along with improving constantly of rapid economy development and living standards of the people, China's total energy consumption also increases sharply.95% is fossil energy at present used commercial energy resource, but because the environmental problem that causes in the exploitation of fossil energy resources process is seriously restricting Sustainable development.The fossil energy resource consumes fast in the world, under the situation that environmental pollution is serious day by day and the climate warming threat increases gradually, for support that Chinese Government proposes to the gross domestic product Carbon emission of the year two thousand twenty unit than 2005 less 40% to 45% target, the development and use of renewable energy sources such as hydrogen especially will be paid much attention to.Hydrogen has alleviated the pollution to environment widely as a kind of novel energy of cleaning, has protected the natural eubiosis, and itself is renewable, and reserves are very abundant, and the energy density height is 2175 times of gasoline, and heat conversion is also very high.Therefore, it promises to be following human clean energy most.
Utilize the downstream system with respect to perfect day by day Hydrogen Energy, hydrogen be not to realize breaking through aspect the raw material production with the renewable resources not but.At present, the hydrogen main source still is reformation conversion (accounting for 96%) and the water electrolysis hydrogen production (accounting for 4%) of fossil oil, and this obviously fails to break away from original fossil energy system.Therefore, how to obtain hydrogen from nature sustainably and especially be subjected to people's attention.Biological hydrogen production is one of important channel that addresses this problem.The research of modern biological hydrogen production starts from the energy dilemma of the seventies in 20th century, to the nineties along with further understanding to Greenhouse effect, biological hydrogen production causes people's attention once more as the industrial technology of Sustainable development.Biological hydrogen production comprises optical drive hydrogen manufacturing and two kinds of paths of anaerobically fermenting hydrogen manufacturing.Compare with photosynthetic organism hydrogen generation; anaerobically fermenting hydrogen manufacturing have produce hydrogen rate height, hydrogen-producing speed fast, produce that hydrogen is continual and steady, the design operation of reaction unit is simple, raw material sources are extensive and characteristics such as cost is low; be easier to accomplish scale production, thereby become the main direction of biological hydrogen production research.Anaerobically fermenting hydrogen manufacturing bacterial classification comprises obligatory anaerobic bacteria and facultative anaerobe, wherein, and the cultivation of obligatory anaerobic bacteria, transportation and industrialized condition harshness, and facultative anaerobe has adaptability widely, is the better bacterium of biological hydrogen production industrialization.At present, the facultative anaerobe kind is more single, lacks new mushroom-seed culturing, and hydrogen generation efficiency is on the low side, and the hydrogen manufacturing condition is treated further optimization.
By patent retrieval, at present existing patent mainly is the research to fermentation substrate and fermentation unit, preparation method's (power etc. is built in 01140458.2 Shen) as the plant straw biological hydrogen production fermentation liquor, the method of a kind of mud and the hydrogen manufacturing of organic waste mixed biologic (application number: 200710032658.0 weeks are strange less etc.), the 00231651.X power of building in Shen) and efficient fermentation method biological hydrogen production expanded bed equipment (application number: 03260012.7 Nan Qi etc.) etc. fermentative hydrogen production device (application number:, and for fermented bacterium particularly a little less than the screening study relative thin of highly effective hydrogen yield bacterial classification, a kind of biological hydrogen production method and special bacterium thereof (application number: 200910088408.8 Xing Xin can wait) are only arranged, but the used bacterial classification of this method is the reorganization bacterium after importing by gene, operation is complicated, and the strict anaerobism of fermentation condition is difficult for promoting.So, optimize extremely urgent for the screening of amphimicrobian new mushroom-seed culturing and the exploration of process for making hydrogen condition.
Summary of the invention
In order to solve the biological hydrogen production method complicated operation that prior art exists, the problem that fermentation condition is strict, the present invention mainly comprises a kind of method of utilizing facultative anaerobe hydrogen manufacturing, this method is under non-strictly anaerobic condition,, use enteroaerogen (Enterobacter aerogenes) CICC10293, be fermentation substrate with glucose, adopt mode of batch fermentation, can efficiently finish hydrogen production process in 20 hours.
Technical scheme of the present invention is: the selected facultative anaerobe of the present invention is specially enteroaerogen (Enterobacteraerogenes) CICC10293 of enterobacteriaceae (Enterobacteriaceae) enterobacter (Enterobacter), this Pseudomonas prototroph amphimicrobian Gram-negative bacteria, available from Chinese industrial microbial strains preservation center (China Center of Industrial Culture Collection, CICC).
A kind of method of utilizing facultative anaerobe fermentation hydrogen manufacturing, use enteroaerogen (Enterobacteraerogenes) CICC10293 of facultative anaerobe as enterobacteriaceae (Enterobacteriaceae) enterobacter (Enterobacter), with peptone, extractum carnis, sodium-chlor, the mixed solution of water is a substratum, with glucose is fermentation substrate, at the fermentation initial stage, preferably can guarantee to be in anaerobism or nearly anaerobic environment, adopt the method for batch fermentation, prepare hydrogen, wherein, the concentration of peptone is 4～6g/L in the described substratum, extractum carnis is 2～4g/L, NaCl is 4～6g/L, does not just need in the fermentation after producing gas to have guaranteed to be in anaerobism or nearly anaerobic environment, and do not have what requirement to the oxygen concentration in the environment this moment.
Concrete grammar is: under the aseptic technique, preparation substratum and sterilization are inoculated in substratum with the highly enriched bacterium liquid of enteroaerogen with 2%～5% volume ratio, and the bacterial concentration of described highly enriched bacterium liquid is 5 * 10
9～5 * 10
11Cell/mL, be 20～50 ℃ in temperature, keep pH 5～8, behind shaking table shaking culture 24～48h of 170r/min, it is in 5～60g/L glucose fermentation substrate that the cultured bacterium liquid that obtains is transferred to sterilized concentration, continue in 20～50 ℃ of temperature, rotating speed 170r/min shaking table shaking culture the sealing back, and connect hydrogen collection device collection hydrogen.
Preferred method is under aseptic technique, in substratum, add the glucose fermentation substrate according to 5～60g/L ratio, obtain the cultivation and fermentation base after the sterilization, the highly enriched bacterium liquid of enteroaerogen is seeded to the cultivation and fermentation base with 2%～5% volume ratio, sealing back is in temperature is 20～50 ℃, the scope of pH 5～8, the shaking table shaking culture of 170r/min, and connect hydrogen reception acquisition means hydrogen.
In substratum, add K
2HPO
41～1.5g/L, FeSO
44H
2O 0.1～0.2g/L produces the hydrogen effect can be better.Control reaction process pH is better at 6～7 hydrogen-producing characteristics in the reaction.
Enteroaerogen (Enterobacter aerogenes) CICC10293 is a facultative anaerobe, requires not strict to culture environment oxygen.The principal element that influences hydrogen production through anaerobic fermentation is: bacterial classification inoculation mode, substratum configuration, fermentation substrate concentration, pH and temperature etc.The present invention is through testing multiple fermentation condition combining and configuring, and comparative analysis finds that following optimum process condition can reach the optimization of hydrogen generation efficiency: the substratum preparation is specially peptone 5g/L, extractum carnis 3g/L, NaCl 5g/L, K
2HPO
41.5g/L, FeSO
44H
2O 0.2g/L; Glucose is fermentation substrate, and concentration is 20g/L; Fermentation pH is 6.0～7.0; Adopt the most basic batch fermentation mode, carry out constant-temperature shaking culture under 40 ℃ of conditions, oscillation frequency is 170r/min, and incubation time is about 20h, produces to gas to stop.Through test, hydrogen output can reach 134mLH
2/ 100mL liquid nutrient medium, the hydrogen generation efficiency height, low to the fermentation condition requirement, have broad application prospects.
Beneficial effect:
At first, pick out the best ferment for hydrogen production bacterial classification of a kind of effect in existing various aerogenesis bacterial classifications, this bacterial classification is a facultative anaerobe, has growth rapidly, and the hydrogen generation efficiency height requires advantages such as low to anaerobic condition;
Secondly, only require at anaerobism or nearly anaerobic environment at the fermentation initial stage, behind the generation gas, just so not strict to environment requirement, non-anaerobic condition just equally can normally ferment down;
Once more, at large optimize the best technological condition for fermentation of this bacterial classification, reached the utilization ratio and the hydrogen generation efficiency maximization of fermentation substrate, determine that fermentation substrate is that concentration is the glucose of 20g/L, substratum is formulated as: peptone 5g/L, extractum carnis 3g/L, NaCl 5g/L, K
2HPO
41.5g/L, FeSO44H
2O 0.2g/L is 6.0～7.0 at pH, and temperature is to adopt mode of batch fermentation to carry out constant-temperature shaking culture under 40 ℃ of conditions, and oscillation frequency is 170r/min, and incubation time is about 20h, and hydrogen output can reach 134mLH
2/ 100mL liquid nutrient medium.
Description of drawings
Fig. 1 is the used experimental installation sketch of the present invention.
Wherein, 1 is the liquid sampling mouth, and 2 is reaction flask, and 3 is the purification for gas bottle, and 4 are the gas sampling mouth, and 5 is gas collector, and 6 is leveling bottle.
Fig. 2 is a hydrogen output variation diagram in time.
Fig. 3 is different fermentations concentration of substrate hydrogen output figure.
Fig. 4 is hydrogen output figure under the differing temps.
Fig. 5 is a hydrogen output variation diagram in time under the different pH values.
Embodiment
The preparation of highly enriched bacterium liquid:
CICC10293 is inoculated in the beef extract-peptone nutrient solution with bacterial strain enteroaerogen (Enterobacter aerogenes), every liter of nutrient solution contains peptone 4～6g, extractum carnis 2～4g, sodium-chlor 4～6g, distilled water 1000mL, and control pH is 6～8, cultivate 24～48h down in 30 ℃, obtain highly enriched bacterium liquid, bacterial concentration is 5 * 10
9～5 * 10
11Cell/mL.
The used experimental installation sketch of the present invention as shown in Figure 1, wherein, 1 is the liquid sampling mouth, 2 is reaction flask, 3 is the purification for gas bottle, 4 for the gas sampling mouth, 5 is gas collector, 6 is leveling bottle.Fill substratum in the reaction flask 2 and as the glucose of substrate.Fill saturated sodium hydroxide solution in the purification for gas bottle 3, gas collector is used for collecting gas.
Embodiment 1: the selection of bacterial classification inoculation mode is determined
Nutrient media components is: peptone 5g/L, extractum carnis 3g/L, NaCl 5g/L regulates initial pH=7.0, with this as basic medium; All sterilizations are 115 ℃ of high-temperature sterilization 25min;
(1) scheme one: preparation basic medium and sterilization, the highly enriched bacterium liquid of Enterobacter aerogenes is inoculated in basic medium (aseptic technique) with 2%～5% volume ratio, after temperature is 30 ℃, shaking table shaking culture 24～48h of 170r/min, it is in the 20g/L glucose fermentation substrate that the cultured bacterium liquid that obtains is transferred to sterilized concentration, the fermented liquid total amount is 450mL, continue in 40 ℃ of temperature, rotating speed 170r/min shaking table shaking culture the sealing back, and connect the hydrogen collection device.
(2) scheme two: add glucose as fermentation substrate according to the 20g/L ratio to basic medium, obtain the cultivation and fermentation base after the sterilization, with the highly enriched bacterium liquid of Enterobacter aerogenes with 2%～5% volume ratio direct inoculation to cultivation and fermentation base (aseptic technique), the fermented liquid total amount is 450mL, and sealing back is 40 ℃, the shaking table shaking culture of 170r/min and connects hydrogen and receive acquisition means in temperature.
At the fermentation initial stage, all with purging with nitrogen gas fermentation and hydrogen production device 3～5min, assurance device is in anaerobism or nearly anaerobic state.In culturing process, collect gas and real time record gas yield, regularly get the fermented liquid sample and measure indexs such as pH and breakdown of glucose rate; Hydrogen content is measured and is adopted gas chromatograph TCD thermal conductance device to detect; PH measures and adopts the pH acidometer; The breakdown of glucose rate adopts film colorimetric method for determining.
Experimental result shows: about 6 hours of scheme one aerogenesis total duration, and aerogenesis speed is fast, and pH reduces rapidly, hydrogen output 212mL, hydrogen ratios 20%, breakdown of glucose rate are 71%; About 20 hours of scheme two aerogenesis total durations are initially breeding stage of bacterial classification, and gas production rate is low, and is the highest at 6～16 hours gas production rates, and the pH value slowly reduces, hydrogen output 494mL, and hydrogen ratios 34.5%, breakdown of glucose rate are 83.05%.Contrast finds that scheme two has higher hydrogen generation efficiency advantage, is suitable for using in the fermenting process.
Embodiment 2: what the basic component of best fermentation disposed determines
According to the result of embodiment 1, on application scheme two bases, further fermentation condition is optimized, explore best fermention medium component configuration, carry out following test:
Prepare 3 kinds of fermention mediums respectively, be numbered 1～3, its composition sees the following form respectively:
| The substratum numbering | Glucose 20g/L | Peptone 5g/L | Extractum carnis 3g/L | ??NaCl??5g/L | ??K 2HPO 4??1.5g/L | ??FeSO 4·4H 2O??0.2g/L |
| ??1 | ??+ | ??+ | ??+ | ??+ | ? | ? |
| ??2 | ??+ | ??+ | ??+ | ??+ | ??+ | ? |
| ??3 | ??+ | ??+ | ??+ | ??+ | ??+ | ??+ |
Regulate the initial pH=7.0 of fermention medium, in 115 ℃ of high-temperature sterilization 25min, the highly enriched bacterium liquid of Enterobacter aerogenes is inoculated (aseptic technique) with 2%～5% volume ratio, the fermented liquid total amount is 450mL, and fermentation condition is all identical.Before cultivating beginning, all being placed on temperature with purging with nitrogen gas 3～5min to anaerobism or nearly anaerobic environment is 40 ℃, and the shaking table shaking culture of 170r/min also connects the hydrogen collection device, and the time length is 20 hours.Collect gas in the fermenting process, and the real time record gas yield; Hydrogen content is measured and is adopted gas chromatograph TCD thermal conductance device to detect; PH measures and adopts the pH acidometer; The breakdown of glucose rate adopts film colorimetric method for determining, and hydrogen output changes in time sees accompanying drawing 2.
Found through experiments, numbering 1,2,3 substratum hydrogen outputs are respectively: 229mL, 337mL, 494mL.Grape sample rate of decomposition is 55.3%, 76%, 83.05%.Experiment shows, a certain amount of buffered soln K
2HPO
4, can obviously reduce speed and amplitude that the pH value descends, acidity is maintained in the scope that is fit to the enteroaerogen growth activity, be beneficial to the generation of producing the hydrogen process; Described in document, add the Fe of proper concn simultaneously
2+Promote to produce the generation of hydrogen enzyme, promoted to produce hydrogen; Compare with numbering 1, the fermention medium component configuration of numbering 3 can improve hydrogen output more than 50%.
Embodiment 3: the determining of best fermentation substrate concentration
According to the result of embodiment 1 and 2,, explore the possibility that improves hydrogen generation efficiency by changing the fermentation substrate starting point concentration.
The preparation basic medium consists of: peptone 5g/L, extractum carnis 3g/L, NaCl 5g/L, K
2HPO
41.5g/L, FeSO
44H
2O 0.2g/L is provided with 4 groups of parallel laboratory tests, sets the initial substrate glucose concn respectively and be 5,10,20,30,40 and 60g/L, and the liquid measure of always fermenting is 450mL, and other fermentation conditions are all identical.Before cultivating beginning, all with purging with nitrogen gas 3～5min to anaerobism or nearly anaerobic environment, placing temperature is 40 ℃, the shaking table shaking culture of 170r/min also connects the hydrogen collection device, the time length is 20 hours.Collect gas in the fermenting process, and the real time record gas yield; Hydrogen content is measured and is adopted gas chromatograph TCD thermal conductance device to detect; PH measures and adopts the pH acidometer; The breakdown of glucose rate adopts film colorimetric method for determining, and different fermentations concentration of substrate hydrogen output is seen accompanying drawing 3.
Find that by test different concns substrate hydrogen output is respectively: 234mL, 329mL, 494mL, 466mL, 414mL, 322mL, when concentration of substrate was 20g/L, hydrogen output was the highest.When concentration of substrate was low, it was less to be utilized glucose amount, and concentration of substrate is too high, and the hydrogen output journey reduces trend rapidly, and this is because concentration of substrate can cause the change of fermentation mode or aerodynamic force diffusion.
Embodiment 4: the determining of optimum fermentation temp
Result according to embodiment 1,2 and 3, according to optimal medium component configuration preparation, under the fermentation substrate concentration conditions, regulating initial pH is 7.0, total fermentation liquid measure is 450mL, and 5 groups of parallel tests are set, and 20,30,35,40,50 ℃ of envrionment temperatures are set respectively, in the shaking table shaking culture of 170r/min and connect the hydrogen collection device, the time length is 20 hours.Before cultivating beginning, all with purging with nitrogen gas 3～5min to anaerobism or nearly anaerobic environment.Collect gas in the fermenting process, and the real time record gas yield; Hydrogen content is measured and is adopted gas chromatograph TCD thermal conductance device to detect; PH measures and adopts the pH acidometer; The breakdown of glucose rate adopts film colorimetric method for determining, and hydrogen output is seen accompanying drawing 4 under the differing temps.
Experiment shows that hydrogen generation efficiency is the highest under 40 ℃ of conditions, reaches 494mL, and temperature is too high or cross the low hydrogen production potential reduction that all can cause because of the activity that influence bacterium.
Embodiment 5: best fermentation pH determines
(1) according to the result of embodiment 1 and 2,, carry out following test for exploring best fermentation pH condition:
The fermention medium component is numbering 3 nutrient media components configuration among the embodiment 2, and 4 groups of parallel laboratory tests are set, and to regulate initial pH respectively be 5,6,7 and 8 by adding an amount of NaOH and HCl, and the fermented liquid total amount is that other fermentation conditions of 450mL are all identical.Before cultivating beginning, all placing temperature with purging with nitrogen gas 3～5min to anaerobism or nearly anaerobic environment is 40 ℃, and the shaking table shaking culture of 170r/min also connects the hydrogen collection device, and the time is 20 hours.Collect gas in the fermenting process, and the real time record gas yield; Hydrogen content is measured and is adopted gas chromatograph TCD thermal conductance device to detect; PH measures and adopts the pH acidometer; The breakdown of glucose rate adopts film colorimetric method for determining, and hydrogen output changes in time sees accompanying drawing 5.
Found through experiments, find that initial pH is at 6,7 o'clock, hydrogen output is respectively 428mL, 494mL, has higher hydrogen generation efficiency.
(2) on above-mentioned experimental basis, further carry out following test:
The fermention medium component is the nutrient media components configuration of numbering 3 among the embodiment 2, and 2 groups of simultaneous tests are set, and all regulating initial pH value is 7.0, and the fermented liquid total amount is that the 450mL fermentation condition is all identical.Before cultivating beginning, all placing temperature with purging with nitrogen gas 3～5min to anaerobism or nearly anaerobic environment is 40 ℃, and the shaking table shaking culture of 170r/min also connects the hydrogen collection device, and the time is 20 hours.Wherein one group will be designed by pH acidometer The real time measure fermented liquid pH value, and keep approximately within 6～7 scopes by adding acid-alkali accommodation pH, and another group does not process, in contrast; Collect gas in the fermenting process, and the real time record gas yield; Hydrogen content is measured and is adopted gas chromatograph TCD thermal conductance device to detect; PH measures and adopts the pH acidometer; The breakdown of glucose rate adopts film colorimetric method for determining, and hydrogen output changes in time sees accompanying drawing 5.
Found through experiments, keep fermented liquid acidity to reach 600mL at 6～7 scope hydrogen outputs, the breakdown of glucose rate is 97%, is beneficial to the lasting of fermentation and hydrogen production and efficiently carries out, and control group pH reduces rapidly, and the reduction of pH value influences the activity of bacterium and causes hydrogen production potential to reduce.
In sum, enteroaerogen (Enterobacter aerogenes) CICC10293 is as amphimicrobian hydrogenogens kind, can be used for fermentation substrate is in the ferment for hydrogen production process of glucose, find that by test its best technological condition for fermentation is that 1. best bacterial classification inoculation is not when opportunity, basic medium coexisted with fermentation substrate; 2. optimal medium is formulated as: peptone 5g/L, extractum carnis 3g/L, NaCl 5g/L, K
2HPO
41.5g/L, FeSO44H
2The concentration of the 3. best fermentation substrate glucose of O 0.2g/L be 20g/L 4. optimum fermentation temp be 40 ℃; 5. the initial pH of best fermentation is 7, and the balance of keeping fermentation fermentation pH is beneficial to the generation of producing hydrogen.
Claims (6)
1. method of utilizing facultative anaerobe fermentation hydrogen manufacturing, it is characterized in that, use enteroaerogen (Enterobacteraerogenes) CICC10293 of facultative anaerobe as enterobacteriaceae (Enterobacteriaceae) enterobacter (Enterobacter), mixed solution with peptone, extractum carnis, sodium-chlor, water is a substratum, with glucose is fermentation substrate, adopt the method for batch fermentation, prepare hydrogen, wherein, the concentration of peptone is 4～6g/L in the described substratum, extractum carnis is 2～4g/L, and NaCl is 4～6g/L.
2. according to the described method of utilizing facultative anaerobe fermentation hydrogen manufacturing of claim 1, it is characterized in that, concrete grammar is: under the aseptic technique, preparation substratum and sterilization, the highly enriched bacterium liquid of enteroaerogen is inoculated in substratum with 2%～5% volume ratio, and the bacterial concentration of described highly enriched bacterium liquid is 5 * 10
9～5 * 10
11Cell/mL, be 20～50 ℃ in temperature, keep pH 5～8, behind shaking table shaking culture 24～48h of 170r/min, it is in 5～60g/L glucose fermentation substrate that the cultured bacterium liquid that obtains is transferred to sterilized concentration, continue in 20～50 ℃ of temperature, rotating speed 170r/min shaking table shaking culture the sealing back, and connect hydrogen collection device collection hydrogen.
3. according to the described method of utilizing facultative anaerobe fermentation hydrogen manufacturing of claim 2, it is characterized in that, under the aseptic technique, in substratum, add the glucose fermentation substrate according to 5～60g/L ratio, obtain the cultivation and fermentation base after the sterilization, the highly enriched bacterium liquid of enteroaerogen is seeded to the cultivation and fermentation base with 2%～5% volume ratio, the sealing back is in temperature is 20～50 ℃, the scope of pH 5～8, the shaking table shaking culture of 170r/min, and connect hydrogen reception acquisition means hydrogen.
4. according to the arbitrary described method of utilizing facultative anaerobe fermentation hydrogen manufacturing of claim 1～3, it is characterized in that, in substratum, add K
2HPO
41～1.5g/L, FeSO
44H
2O 0.1～0.2g/L.
5. according to the described method of utilizing facultative anaerobe fermentation hydrogen manufacturing of claim 4, it is characterized in that pH is 6～7.
6. according to the described method of utilizing facultative anaerobe fermentation hydrogen manufacturing of claim 5, it is characterized in that the concentration of each composition is in the described fermention medium: peptone 5g/L, extractum carnis 3g/L, NaCl 5g/L, K
2HPO
41.5g/L, FeSO
44H
2O 0.2g/L; The glucose concentration of substrate is 20g/L; Be 6.0～7.0 at pH, temperature is to adopt mode of batch fermentation to carry out constant-temperature shaking culture under 40 ℃ of conditions, oscillation frequency is 170r/min, incubation time 20h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201019026129 CN101824437A (en) | 2010-03-02 | 2010-03-02 | Method for producing hydrogen by utilizing facultative anaerobe fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201019026129 CN101824437A (en) | 2010-03-02 | 2010-03-02 | Method for producing hydrogen by utilizing facultative anaerobe fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101824437A true CN101824437A (en) | 2010-09-08 |
Family
ID=42688601
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201019026129 Pending CN101824437A (en) | 2010-03-02 | 2010-03-02 | Method for producing hydrogen by utilizing facultative anaerobe fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101824437A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242074A (en) * | 2011-04-12 | 2011-11-16 | 叶春 | Screen method for facultative anaerobe, and apparatus thereof |
| CN115960970A (en) * | 2022-11-22 | 2023-04-14 | 哈尔滨工业大学 | Method for producing hydrogen by fermenting human excrement |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101294167A (en) * | 2008-06-23 | 2008-10-29 | 清华大学 | Method for preparing hydrogen gas |
| WO2009062119A2 (en) * | 2007-11-07 | 2009-05-14 | Sustainable Green Technologies, Inc. | Microorganisms and methods for increased hydrogen production |
| KR20090082779A (en) * | 2008-01-28 | 2009-07-31 | 고려대학교 산학협력단 | Method for hydrogen production by sequential culture of anaerobes |
| JP2009183162A (en) * | 2008-02-04 | 2009-08-20 | Hiroshima Univ | New enterobacter aerogenes strain and its utilization |
-
2010
- 2010-03-02 CN CN 201019026129 patent/CN101824437A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009062119A2 (en) * | 2007-11-07 | 2009-05-14 | Sustainable Green Technologies, Inc. | Microorganisms and methods for increased hydrogen production |
| KR20090082779A (en) * | 2008-01-28 | 2009-07-31 | 고려대학교 산학협력단 | Method for hydrogen production by sequential culture of anaerobes |
| JP2009183162A (en) * | 2008-02-04 | 2009-08-20 | Hiroshima Univ | New enterobacter aerogenes strain and its utilization |
| CN101294167A (en) * | 2008-06-23 | 2008-10-29 | 清华大学 | Method for preparing hydrogen gas |
Non-Patent Citations (2)
| Title |
|---|
| 《化工进展》 20060728 卢文玉等 激光诱变选育耐酸产氢菌产气肠杆菌 第25卷, 第07期 2 * |
| 《环境研究与监测》 20080320 刘婧 一株厌氧发酵菌Biolog鉴定及利用不同碳源对产氢的影响 第21卷, 第01期 2 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102242074A (en) * | 2011-04-12 | 2011-11-16 | 叶春 | Screen method for facultative anaerobe, and apparatus thereof |
| CN102242074B (en) * | 2011-04-12 | 2014-08-06 | 叶春 | Screen method for facultative anaerobe |
| CN115960970A (en) * | 2022-11-22 | 2023-04-14 | 哈尔滨工业大学 | Method for producing hydrogen by fermenting human excrement |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kotay et al. | Microbial hydrogen production with Bacillus coagulans IIT-BT S1 isolated from anaerobic sewage sludge | |
| Pandu et al. | Comparisons and limitations of biohydrogen production processes: a review | |
| Saraphirom et al. | Biological hydrogen production from sweet sorghum syrup by mixed cultures using an anaerobic sequencing batch reactor (ASBR) | |
| Zhang et al. | Improvement of biohydrogen production from dark fermentation by cocultures and activated carbon immobilization | |
| CN104164395B (en) | Clostridium beijerinckii for hydrogen generation via fermentation as well as fermentation method and application of clostridium beijerinckii | |
| CN105462874B (en) | A kind of clostridium butyricum and its application in hydrogen production through anaerobic fermentation | |
| Xia et al. | Sequential generation of hydrogen and methane from glutamic acid through combined photo-fermentation and methanogenesis | |
| CN103421714B (en) | Bacillus shackletonii and application thereof in fermentation production of polyhydroxybutyrate | |
| Wang et al. | Efficiency enhancement of H2 production by a newly isolated maltose-preferring fermentative bio-hydrogen producer of Clostridium butyricum NH-02 | |
| KR20200108745A (en) | System for Biofuel production and Manufacturing method thereof | |
| CN110106223A (en) | A method of promoting corn stover photosynthetic hydrogen production | |
| Sagnak et al. | Hydrogen gas production from acid hydrolyzed wheat starch by combined dark and photo-fermentation with periodic feeding | |
| CN102041274A (en) | Method for producing hydrogen by fermenting special anaerobic clostridium butyricum | |
| Ust’ak et al. | Experimental verification of various methods for biological hydrogen production | |
| CN104673712A (en) | Bacterial strain for producing alcohol fuels by synchronously utilizing glucose and xylose and application of bacterial strain | |
| CN101531979B (en) | Klebsiella pneumoniae and method of preparing hydrogen and 2, 3-butanediol thereby | |
| CN101824437A (en) | Method for producing hydrogen by utilizing facultative anaerobe fermentation | |
| CN101988075B (en) | Method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum | |
| CN1321174C (en) | Continuous flow culture method of industrial biological hydrogen preparing spawn and biological hydrogen preparing system reinforcing method | |
| CN103993041A (en) | Method for improving hydrogen production by microalgae | |
| Zhao et al. | Hydrogen production characteristics from dark fermentation of maltose by an isolated strain FP 01 | |
| Zhang et al. | Experiment of integrated fermentation hydrogen production by photosynthetic bacteria cooperating with Enterobacter aerogenes | |
| CN105754889B (en) | A kind of enterococcus faecium and its application in hydrogen production through anaerobic fermentation | |
| CN114015602A (en) | Preparation method of biomass photosynthetic hydrogen production mixed flora | |
| CN101886040A (en) | Method for preparing hydrogen-producing and ethanol-producing microbial aggregate |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20100908 |