CN101817091A - Method for preparing iron nano-magnetic particles by taking T4 phage as template - Google Patents
Method for preparing iron nano-magnetic particles by taking T4 phage as template Download PDFInfo
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Abstract
The invention discloses a method for preparing iron nano-magnetic particles by taking a T4 phage as a template in the field of magnetic recording materials. In the method, the T4 phage obtained by enrichment and the solution of iron chloride are incubated, centrifuged and reduced by utilizing intrinsic structural characteristics of an organism and a molecular recognition function thereof to obtain iron nano-particles which have small particle sizes and high dispersion degrees and are regularly distributed on the capsid surface of the T4 phage, and the prepared iron nano-particles have the characteristics of nonreversible magnetization process, high coercive force and the like, so that the iron nano-particles prepared by the method have wide application prospect in the field of high-intensity magnetic recording materials. Moreover, in the method, a biological nano-structure which exists in nature is directly taken as the template, so that the method has simple preparation processes, moderate reaction conditions and environment friendliness. Since a biological template has the characteristics of self-producing capability, high appearance repeatability and the like, large scale production is easy to realize.
Description
Technical field
The invention belongs to the magnetic recording material field, being specifically related to a kind of is that template prepares small particle diameter and has the method for ferromagnetic Fe nanometer particles simultaneously with the T4 bacteriophage.
Background technology
Transition metal iron (Fe) nano particle has characteristics such as size is little, single domain structure, coercivity height, the magnetic recording material of making of its has advantages such as good stability, clear image, signal to noise ratio height, the distortion factor are little, just because of these, make nano magnetic material wide application prospect be arranged in high density magnetic recording material field.But along with the development of information technology, need the information content of record also constantly to increase, therefore more and more higher to the requirement of magnetic recording material, require the recording materials high performance, particularly write down densification.And as the magnetic nanoparticle of magnetic recording unit, its size must satisfy following requirement: 1. the length of particle should be much smaller than recording wavelength; 2. the width of particle (as possibility, in length is also included within) should be much smaller than registered depth; 3. in the recording volume of a unit, more magnetic particle should be arranged as far as possible.To sum up, as the nano particle of magnetic recording material, satisfy size and try one's best little but still keep ferromagnetic requirement.Therefore, probe into and a kind ofly can prepare small size and have the research focus that ferromagnetic Fe nanometer particle process method just becomes people, also become information industry competition focal point.In recent years, people utilize diverse ways to prepare the Fe magnetic nanoparticle, and the most frequently used is the pyrolysismethod of using organo-metallic compound.This method selects for use organo-metallic compound as predecessor, and (usually in 180～300 ℃ of scopes) thermal degradation at high temperature obtains metal nanoparticle.For example, Sheng Peng etc. is published in " JACS " (JACS, 2006,128,10676～10677) and Dorothy Farrell etc. be published in " physical chemistry " (J.Phys.Chem.B, 2005,109,13409～13419) all reported organic compound Fe (CO) in the article with iron
5Be presoma, thermal degradation has prepared the Fe nano particle, and the particle diameter of product is less, and it is also better that interparticle distance is controlled.Mostly be virulent organic solvent greatly but this method is raw materials used, and synthesis condition needing hot environment, is the disagreeableness synthetic route of a kind of environment therefore.People also adopt the oleate of iron to make presoma and obtain the Fe nano particle through thermal degradation, but the particle morphology of preparation is irregular, and particle diameter is relatively large, does not have ferromagnetic characteristic, and this has just limited the Fe nano particle in the magnetic recording material Application for Field.
Summary of the invention
In order to overcome conventional preparation method's deficiency, the invention provides a kind of is the method that template prepares the Fe magnetic nanoparticle with the T4 bacteriophage.This method preparation technology is simple, productive rate is high, the Fe nano particle of preparing have particle diameter little, in T4 phage surface arranging rule, decentralization advantages of higher, the Fe nano particle of preparing simultaneously have magnetic history irreversible, ferromagnetic characteristics such as hysteresis, coercivity height are arranged, therefore, products obtained therefrom satisfies the requirement of high density magnetic recording material.And the biological nano structure that this method directly adopts nature to exist is template, and raw material sources are extensive, cultivate simple, thereby greatly reduce production cost, raw materials used also nonhazardous is a kind of eco-friendly preparation method.
The present invention adopts highly, and the nano grade biological body of symmetry is a template, utilize the architectural feature of himself, with and the molecule distinguishability of surface protein height, has the principle of intermolecular force according to transition metal cation and amino acid carboxyl anion, at its surface control growth nano-particle, because space confinement effect and the architectural feature thereof of organism, can be effectively to the synthetic accuracy controlling of carrying out of nano particle, thereby obtain the desired size size, monodispersed Fe magnetic nanoparticle.
Less and have ferromagnetic Fe nano particle in order to obtain particle diameter, it is template that the present invention adopts the T4 bacteriophage of natural type, at the synthetic Fe nano particle of its surface control.The T4 bacteriophage is the colibacillary virulent phage of a kind of dip-dye, is host cell with Escherichia coli, very is convenient to cultivate.And Escherichia coli are main and a kind of bacteriums that quantity is maximum in people and the many animal intestinals, and are generally not pathogenic, are template so adopt the T4 bacteriophage, and it is extensive to have a material source, repeatable advantages of higher.The used T4 bacteriophage of the present invention is the icosahedron symmetrical structure, capsid is about 60nm, the about 50nm of transverse diameter, owing to contain several amino acids on its surface, for providing many binding sites at the synthetic Fe nano particle of its surface control, and the repeatability that the particular combination site is rule distributes, so the nano particle of preparation has good dispersiveness.Because the space confinement effect of the protein of its protrusion of surface, controlled the size of synthetic nano particle again, the nano particle size that makes preparation evenly and particle diameter very little.As seen because himself architectural feature of T4 bacteriophage makes it become the good template of preparation Fe magnetic nanoparticle.
The present invention at first utilizes Escherichia coli to cultivate the T4 bacteriophage, through repeatedly centrifugal, the enrichment of pure T4 bacteriophage is got up, hatch altogether with the chloride solution of Fe the back, make the cation of iron be adsorbed onto on the avtive spot of T4 capsid surface protein amino acid carboxyl anion, by centrifugal, reduction is handled then, just formed on T4 bacteriophage capsid surface arranging rule, particle diameter less, have a ferromagnetic Fe nano particle.
Technical scheme of the present invention comprises the steps:
(1) amplification cultivation of T4 bacteriophage and purification
At first at the 50ml that cultivates in advance, concentration is to insert T4 bacteriophage bacterial classification in the Escherichia coli suspension of 10～15mg/ml, under 37 ℃, cultivates 8～15h with the speed shaking table of 100～150r/min.The suspension that will contain residual shell of Escherichia coli and T4 bacteriophage is distinguished centrifugal three times with the speed of 4000～6000r/min under 4～10 ℃, and each centrifugal 15～20min all removes precipitation, gets supernatant.T4 phagocytosis body fluid that at last will be purer is at 4～10 ℃, and ultracentrifugation 2.5～3h removes supernatant under 40000～45000r/min, and T4 bacteriophage precipitation spot is distributed in the 2ml deionized water.
(2) be that template prepares the Fe magnetic nanoparticle with the T4 bacteriophage
Get the T4 phagocytosis body fluid after 300～500ul enrichment, regulate pH value to 8.5～9.0 with the NaOH of 1mol/l, in 4～10 ℃, shaking table hatching 3～5h under 60～110r/min.Get the FeCl that 300～500ul concentration is 5～10mM
3Solution joins in the above-mentioned pretreated T4 phagocytosis body fluid, mixes, and in 4～10 ℃, shaking table hatching 12～20h under 60～110r/min.In 4～10 ℃, ultracentrifugation 2.5～3h removes supernatant under 40000～45000r/min then, precipitation is collected be distributed in the deionized water of 300～500ul.Dropwise add 75～120ul of new preparation then, concentration is the NaBH of 5～10mM
4Reductant solution promptly obtains rule and is arranged in T4 bacteriophage capsid surface, small particle diameter and has ferromagnetic Fe nano particle.
Described Fe magnetic nanoparticle has following architectural feature: be distributed in T4 bacteriophage capsid outer surface uniformly, and its arranging rule, high degree of dispersion, particle diameter is less, is of a size of 1.5～4.0nm.
The invention has the beneficial effects as follows: directly adopting natural T4 bacteriophage is template, cultivate easy, need not carry out any specially treated before the experiment, through hatching altogether with the chloride solution of iron, handle through centrifugal, reduction then, can obtain rule and be arranged in T4 bacteriophage capsid surface, small particle diameter and have ferromagnetic Fe nano particle.Making the Fe nano particle that the presoma thermal degradation prepares with the organic compound with iron of routine compares, the Fe nano particle that the present invention obtains has advantages such as distribution is even more, particle diameter is less, key is still to keep good ferromagnetism under small particle diameter, satisfies the requirement of magnetic recording material to magnetic nanoparticle.This preparation method's technology is simple, mild condition, and environment-friendly high-efficiency, and the template wide material sources are easy to get, and with low cost, be easy to realize large-scale production.
Description of drawings
The present invention is further elaborated below in conjunction with description of drawings and embodiment.
Fig. 1 is to be the low multiple TEM figure of the Fe magnetic nanoparticle of template preparation with the T4 bacteriophage;
Fig. 2 is to be the high multiple TEM figure of the Fe magnetic nanoparticle of template preparation with the T4 bacteriophage;
Fig. 3 is to be the EDS figure of the Fe magnetic nanoparticle of template preparation with the T4 bacteriophage;
Fig. 4 is to be the hysteresis curve curve of the Fe magnetic nanoparticle of template preparation with the T4 bacteriophage.
The specific embodiment
Embodiment one
1.T4 amplification cultivation of bacteriophage and purification
At first at the 50ml that cultivates in advance, concentration is to insert T4 bacteriophage bacterial classification in the Escherichia coli suspension of 10mg/ml, under 37 ℃, cultivates 8h with the speed shaking table of 100r/min.The suspension that will contain residual shell of Escherichia coli and T4 bacteriophage under 4 ℃, respectively with 4000,5000, the speed of 6000r/min is centrifugal successively, each centrifugal 15min all removes precipitation, gets supernatant.T4 phagocytosis body fluid that at last will be purer is at 4 ℃, and ultracentrifugation 2.5h removes supernatant under the 45000r/min, and T4 bacteriophage precipitation spot is distributed in the 2ml deionized water.
2. be that template prepares the Fe magnetic nanoparticle with the T4 bacteriophage
Get the T4 phagocytosis body fluid after the 300ul enrichment, regulate pH value to 8.5 with the NaOH of 1mol/l, in 4 ℃, shaking table hatching 3h under the 60r/min.Get the FeCl that 300ul concentration is 5mM
3Solution joins in the above-mentioned pretreated T4 phagocytosis body fluid, mixes, and in 4 ℃, shaking table hatching 20h under the 60r/min.In 4 ℃, ultracentrifugation 2.5h removes supernatant under the 45000r/min then, precipitation is collected be distributed in the deionized water of 300ul.Dropwise add the 75ul of new preparation then, concentration is the NaBH of 5mM
4Reductant solution promptly obtains rule and is arranged in T4 bacteriophage capsid surface, small particle diameter and has ferromagnetic Fe nano particle.Its transmission electron microscope picture is seen Fig. 1.
Embodiment two
1.T4 amplification cultivation of bacteriophage and purification
At first at the 50ml that cultivates in advance, concentration is to insert T4 bacteriophage bacterial classification in the Escherichia coli suspension of 12mg/ml, under 37 ℃, cultivates 12h with the speed shaking table of 130r/min.The suspension that will contain residual shell of Escherichia coli and T4 bacteriophage under 10 ℃, respectively with 4000,5000, the speed of 6000r/min is centrifugal successively, each centrifugal 17min all removes precipitation, gets supernatant.T4 phagocytosis body fluid that at last will be purer is at 10 ℃, and ultracentrifugation 3h removes supernatant under the 42100r/min, and T4 bacteriophage precipitation spot is distributed in the 2ml deionized water.
2. be that template prepares the Fe magnetic nanoparticle with the T4 bacteriophage
Get the T4 phagocytosis body fluid after the 400ul enrichment, with the NaOH adjust pH to 8.5 of 1mol/l, in 10 ℃, shaking table hatching 4h under the 90r/min.Get the FeCl that 400ul concentration is 10mM
3Solution joins in the above-mentioned pretreated T4 phagocytosis body fluid, mixes, and in 10 ℃, shaking table hatching 12h under the 90r/min.In 10 ℃, ultracentrifugation 3h removes supernatant under the 42100r/min then, precipitation is collected be distributed in the deionized water of 400ul.Dropwise add the 120ul of new preparation then, concentration is the NaBH of 6mM
4Reductant solution promptly obtains rule and is arranged in T4 bacteriophage capsid surface, small particle diameter and has ferromagnetic Fe nano particle.Transmission electron microscope is observed its pattern down and is seen Fig. 2.
Embodiment three
1.T4 amplification cultivation of bacteriophage and purification
At first at the 50ml that cultivates in advance, concentration is to insert T4 bacteriophage bacterial classification in the Escherichia coli suspension of 15mg/ml, under 37 ℃, cultivates 15h with the speed shaking table of 150r/min.The suspension that will contain residual shell of Escherichia coli and T4 bacteriophage under 6 ℃, respectively with 4000,5000,6000r/min speed centrifugal successively, each centrifugal 20min all removes precipitation, gets supernatant.T4 phagocytosis body fluid that at last will be purer is at 6 ℃, and ultracentrifugation 3h removes supernatant under the 40000r/min, and T4 bacteriophage precipitation spot is distributed in the 2ml deionized water.
2. be that template prepares the Fe magnetic nanoparticle with the T4 bacteriophage
Get the T4 phagocytosis body fluid after the 500ul enrichment, regulate pH value to 9.0 with the NaOH of 1mol/l, in 6 ℃, shaking table hatching 5h under the 110r/min.Get the FeCl that 500ul concentration is 10mM
3Solution joins in the above-mentioned pretreated T4 phagocytosis body fluid, mixes, and in 6 ℃, shaking table hatching 18h under the 110r/min.In 6 ℃, ultracentrifugation 3h removes supernatant under the 40000r/min then, precipitation is collected be distributed in the deionized water of 500ul.Dropwise add the 90ul of existing preparation then, concentration is the NaBH of 10mM
4Reductant solution promptly obtains rule and is arranged in T4 bacteriophage capsid surface, small particle diameter and has ferromagnetic Fe nano particle.
Fig. 1,2 is for the T4 bacteriophage being the TEM figure of the Fe nano particle of template preparation, as we can see from the figure, class spheroidal grey shadow in bottom is the T4 bacteriophage, at each bacteriophage capsid outer surface one deck Fe nano particle that evenly distributed, particle diameter is less, is of a size of 1.5～4.0nm, the distribution of particles rule, to have proved with the T4 bacteriophage be template has high controllability to the preparation of Fe nano particle on pattern and size, the high efficiency of this method has been described.
Fig. 3 is for the T4 bacteriophage being the EDS figure that template prepares the Fe magnetic nanoparticle, be that electron beam is beaten on the combining structure of T4 bacteriophage that is presented as Fig. 1,2 and metallic, the peak of Cu, C, O, Fe as we can see from the figure, wherein Cu, C mainly are copper mesh used during from sample preparation.O mainly is that some O is from water from T4 bacteriophage itself.In addition, we can see the existence at the power spectrum peak of Fe from figure, illustrate with the T4 bacteriophage be the template success prepared the Fe nano particle.
Fig. 4 is for being the hysteresis curve curve of the Fe magnetic nanoparticle for preparing of template with the T4 bacteriophage, and detected temperatures is 100K.As can be seen from the figure, with the T4 bacteriophage be that the Fe nano particle of template preparation has that magnetic history is irreversible, easy magnetization to saturated, ferromagnetic characteristic such as hysteresis arranged.The about 564Oe of its coercivity, the about 34emu/g of remanent magnetism, the about 151emu/g of saturation magnetization.Explanation is that the Fe nano particle that template prepares has ferromagnetism with the T4 bacteriophage, and this makes it that wide application prospect be arranged aspect magnetic recording material.
Claims (1)
1. one kind is the method that template prepares iron nano-magnetic particles with the T4 bacteriophage, it is characterized in that, described method comprises the steps:
(1) amplification cultivation of T4 bacteriophage and purification
At first at the 50ml that cultivates in advance, concentration is to insert T4 bacteriophage bacterial classification in the Escherichia coli suspension of 10～15mg/ml, under 37 ℃, speed shaking table with 100～150r/min is cultivated 8～15h, the suspension that will contain residual shell of Escherichia coli and T4 bacteriophage is under 4～10 ℃, speed with 4000～6000r/min is distinguished centrifugal three times, each centrifugal 15～20min, all remove precipitation, get supernatant, T4 phagocytosis body fluid that at last will be purer is at 4～10 ℃, ultracentrifugation 2.5～3h under 40000～45000r/min, remove supernatant, T4 bacteriophage precipitation spot is distributed in the 2ml deionized water;
(2) be that template prepares the Fe magnetic nanoparticle with the T4 bacteriophage
Get the T4 phagocytosis body fluid after 300～500ul enrichment, regulate pH value to 8.5～9.0 with the NaOH of 1mol/l, in 4～10 ℃, shaking table hatching 3～5h gets the FeCl that 300～500ul concentration is 5～10mM under 60～110r/min
3Solution joins in the above-mentioned pretreated T4 phagocytosis body fluid, mix, in 4～10 ℃, shaking table hatching 12～20h under 60～110r/min, then in 4～10 ℃, ultracentrifugation 2.5～3h under 40000～45000r/min, remove supernatant, the precipitation collection is distributed in the deionized water of 300～500ul, dropwise adds 75～120ul of new preparation then, concentration is the NaBH of 5～10mM
4Reductant solution promptly obtains rule and is arranged in T4 bacteriophage capsid surface, small particle diameter and has ferromagnetic Fe nano particle.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102658371A (en) * | 2012-03-02 | 2012-09-12 | 燕山大学 | Preparation method of ultrafine platinum nano-wire |
| CN103170641A (en) * | 2013-03-19 | 2013-06-26 | 燕山大学 | Method of preparing platinum nano particles by using cotton bollworm karyotype polyhedrin |
| CN104001933A (en) * | 2014-05-12 | 2014-08-27 | 燕山大学 | Method for preparing gold nanowire by means of cotton bollworm karyotype polyhedrin extractive |
| CN106362151A (en) * | 2016-10-26 | 2017-02-01 | 燕山大学 | Preparation method of Escherichia coli@magnetic particle magnetic-targeted drug carrier |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1480251A (en) * | 2003-07-21 | 2004-03-10 | 天津大学 | Ferromagnetic multiporous silica gel microsphere and its preparation method |
| CN1753724A (en) * | 2002-09-18 | 2006-03-29 | 得克萨斯州立大学董事会 | Peptide mediated synthesis of metallic and magnetic materials |
| US7374893B2 (en) * | 2002-09-18 | 2008-05-20 | Board Of Regents, University Of Texas System- | Peptide mediated synthesis of metallic and magnetic materials |
| CN101619135A (en) * | 2008-07-02 | 2010-01-06 | 中国科学院研究生院 | Magnetic hollow compound microstructure preparation method |
| CN101647077A (en) * | 2007-03-29 | 2010-02-10 | 瑞典树木科技公司 | Magnetic nanoparticle cellulose material |
-
2010
- 2010-04-28 CN CN 201010161190 patent/CN101817091A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1753724A (en) * | 2002-09-18 | 2006-03-29 | 得克萨斯州立大学董事会 | Peptide mediated synthesis of metallic and magnetic materials |
| US7374893B2 (en) * | 2002-09-18 | 2008-05-20 | Board Of Regents, University Of Texas System- | Peptide mediated synthesis of metallic and magnetic materials |
| CN1480251A (en) * | 2003-07-21 | 2004-03-10 | 天津大学 | Ferromagnetic multiporous silica gel microsphere and its preparation method |
| CN101647077A (en) * | 2007-03-29 | 2010-02-10 | 瑞典树木科技公司 | Magnetic nanoparticle cellulose material |
| CN101619135A (en) * | 2008-07-02 | 2010-01-06 | 中国科学院研究生院 | Magnetic hollow compound microstructure preparation method |
Non-Patent Citations (1)
| Title |
|---|
| 《化学进展》 20090131 樊希安等 一维铁磁金属纳米材料的制备、结构调控及其磁性能 第21卷, 第1期 2 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102658371A (en) * | 2012-03-02 | 2012-09-12 | 燕山大学 | Preparation method of ultrafine platinum nano-wire |
| CN102658371B (en) * | 2012-03-02 | 2014-07-23 | 燕山大学 | Preparation method of ultrafine platinum nano-wire |
| CN103170641A (en) * | 2013-03-19 | 2013-06-26 | 燕山大学 | Method of preparing platinum nano particles by using cotton bollworm karyotype polyhedrin |
| CN103170641B (en) * | 2013-03-19 | 2014-12-24 | 燕山大学 | Method of preparing platinum nano particles by using cotton bollworm karyotype polyhedrin |
| CN104001933A (en) * | 2014-05-12 | 2014-08-27 | 燕山大学 | Method for preparing gold nanowire by means of cotton bollworm karyotype polyhedrin extractive |
| CN104001933B (en) * | 2014-05-12 | 2016-03-02 | 燕山大学 | Bollworm polyhedrosis protein extract is utilized to prepare the method for nanowires of gold |
| CN106362151A (en) * | 2016-10-26 | 2017-02-01 | 燕山大学 | Preparation method of Escherichia coli@magnetic particle magnetic-targeted drug carrier |
| CN106362151B (en) * | 2016-10-26 | 2019-05-28 | 燕山大学 | A kind of preparation method of Escherichia coli@magnetic particle magnetic target medicine carrier |
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