CN101810220A - Preparation method of synbiotic goat milk powder - Google Patents
Preparation method of synbiotic goat milk powder Download PDFInfo
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- CN101810220A CN101810220A CN 201010153899 CN201010153899A CN101810220A CN 101810220 A CN101810220 A CN 101810220A CN 201010153899 CN201010153899 CN 201010153899 CN 201010153899 A CN201010153899 A CN 201010153899A CN 101810220 A CN101810220 A CN 101810220A
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Abstract
The invention discloses a preparation method of synbiotic goat milk powder, which comprises the following steps: activating probiotics to obtain probiotic mire; adding a compound freeze-drying protective agent; obtaining freeze-drying probiotic powder by freeze-drying; carrying out purification, standardization and reduced pressure condensation on a raw material of goat milk to obtain new raw material of goat milk; adding prebiotics in the condensed raw material of goat milk and then carrying out spray drying to obtain goat milk powder containing the prebiotics; cooling the goat milk powder to room temperature and filtering with a screen; and mixing the screened goat milk powder containing the prebiotics with the probiotic powder under the aseptic condition to obtain the symbiotic goat milk powder. In the invention, the prebiotics are added into condensed degrease goat milk by condensation or whole goat milk and are beneficial to the uniform dispersion of the prebiotics; the probiotics are prepared by a freeze-drying method and added with the freeze-drying protective agent, so that the viable bacteria is more and bacteria has a colony of over 1,010cfu/g; and by adding the prebiotics into the goat milk powder, the prebiotics can be protected and the survival rate of the prebiotics and the functionality of the goat milk powder can be improved.
Description
Technical field
The present invention relates to a kind of preparation method of functional goat milk powder, be specifically related to a kind of preparation method who contains symphysis unit (Synbiotics) goat milk powder of prebiotics (Prebiotics) and probio (Probiotics).
Background technology
Goat Milk is rich in high-quality protein and aliphatic acid and several mineral materials such as albumin that health is played an important role, globulin, and bad syndrome patient has good health care and therapeutic action to nutrient absorption.Goat milk fat globule little (only be milk 1/3), and be rich in be beneficial to that body directly utilizes in, the short chain unrighted acid, absorptivity is very high.In addition, abundant calcium constituent can help human body burning fat in the goat milk goods, promotes body to produce the enzyme of degraded fat.Studies show that the goat milk calcium content is higher by 15% than milk, it is high 5 times to suckle than the people.Goat milk is rich in superoxide dismutase, and normal food has beauty functions, and has antianaphylactic function.The EGF(EGF that contains in the goat milk), when promoting epithelial cell growth, mucomembranous epithelial cells such as stomach, tracheae and skin there is repair, can urge digestion again can tranquilizing and allaying excitement (because of containing cyclic nucleotide), and can strengthen immunity of organisms, and regulate the human body nutrient balance, improve sleep quality, the comfort cushioning characteristic of its meta-alkalescence also has the effect that promotes rehabilitation to diseases such as ephritis, tuberculosis and asthma, is infant, pregnant woman, old man and patient's excellent health products.
Probio (Probiotics) is meant those by improving its host's intestinal microecology effect and be of value to host's microorganism, probio bacterial classification commonly used have Bifidobacterium (
Bifidobateria), Bacillus acidi lactici and some streptococcus, wherein, Bacillus acidi lactici is commonly used have lactobacillus acidophilus (
Lactobicullusacidophilus), Lactobacillus casei (
L.casei), lactobacillus bulgaricus (
L.bulgaricus) etc.The probio bacterial classification that can be used for health food of China Ministry of Public Health approval at present has: lactobacillus acidophilus, Lactobacillus casei, lactobacillus reuteri, Lactobacillus rhamnosus, bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum, blue and green Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus etc.
Prebiotics (Prebiotics) is meant human body indigestion or heavy composition, alternative growth and the activity that stimulates beneficial bacteria of intestinal tract of these compositions, thereby the host is produced the material of health effect, be mainly functional oligose and a small amount of polysaccharide, as oligomeric maltose, galactooligosaccharide, FOS, isomaltose, milk ketose, xylo-oligosaccharide, stachyose, raffinose, soyabean oligosaccharides, synanthrin etc.
Symphysis unit (synbiotics) is called Synbiotics again, be to be microecologic regulator of new generation, with probio and prebiotics use in conjunction, it both can bring into play the physiological bacterial activity of probio, optionally increase the quantity of probio again, make probiotic action more remarkable lasting, resist disease jointly, safeguard the microecological balance of body.
Patent (CN100506055C) has been announced a kind of red date sheep milk powder and preparation method thereof, and the material of interpolation is an integration of drinking and medicinal herbs material red date.Horsepower etc. (horsepower, Han Xuelin, Xu Weirong. old development with goat milk powder. Food Science, 1993 (1): 26-30) reported a kind of old development, in goat milk powder, added vegetable oil, ferrous lactate, vitamin, carbohydrate etc. with goat milk powder.Yan Peifeng (research of probio Freeze Drying Technique and the application in active goat milk powder [D] thereof, Shaanxi Yang Ling: Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology, 2008) reported active probiotic goat milk powder process technology, by dry pigmentation lactobacillus acidophilus is directly mixed with full-cream goat milk powder, do not relate to prebiotics and add method in the degreasing goat milk powder to.
Summary of the invention
The object of the present invention is to provide a kind of preparation method who contains the synbiotic goat milk powder of probio and prebiotics simultaneously.
To achieve these goals, the technical solution adopted in the present invention is:
First: at first will make probiotics bacterial mud after the probio activation, in probiotics bacterial mud, add the compound freeze drying protectant of identical weight then, in-40 ℃ of pre-freeze 8-12h, move to freeze drier freeze drying 20-24h again and obtain freeze-drying probiotics bacterial powder behind the mixing;
Second: raw material sheep breast after purification and standardization, if the full-cream goat milk powder of preparation symphysis unit directly carried out for the 3rd step, if prepare symphysis unit degreasing goat milk powder, is heated to 36-39 ℃ with raw material sheep breast and carries out degreasing, with fat content control＜0.2%;
The 3rd: the second full-cream sheep breast that obtain of step or degreasing sheep breast reduced pressure to concentrate to be made solid content reach 42-50% to obtain raw material sheep breast;
Raw material sheep Ruzhong after concentrating obtains to contain the degreasing goat milk powder or the full-cream goat milk powder of prebiotics by spray-drying after adding prebiotics, and sieves behind the cool to room temperature; Degreasing goat milk powder that contains prebiotics after will sieving again or full-cream goat milk powder mix with the probiotics bacterial powder under 2-6 ℃, aseptic condition and obtain synbiotic goat milk powder;
Or prebiotics mixed under aseptic condition with the probiotics bacterial powder, cool to room temperature sieves and obtains degreasing goat milk powder or full-cream goat milk powder after raw material sheep after will concentrating the then breast spray-drying, mixed prebiotics and probiotics bacterial powder is directly joined in degreasing goat milk powder or the full-cream goat milk powder make synbiotic goat milk powder;
Said prebiotics addition is by raw material sheep breast 1.5g-6.5g/ liter;
Said probiotics bacterial powder addition is by goat milk powder 1.0-10g/kg.
By the skimmed milk power of 30%-70%, the trehalose of 8%-20%, the glutamic acid of 1%-2%, the glycerine of 1%-2% and the pH of 20-50% are 6.5 citrate buffer solution composition to compound freeze drying protectant of the present invention by mass percentage.
Said probio is that bacterium powder viable count is 1.0 * 10
10-2.0 * 10
11The lactobacillus acidophilus of cfu/g, bacterium powder viable count are 1.0 * 10
10-5.5 * 10
10The Lactobacillus casei of cfu/g, bacterium powder viable count are 1.0 * 10
10-3.4 * 10
10The Lactobacillus plantarum of cfu/g, bacterium powder viable count are 1.0 * 10
10-5.6 * 10
10The Lactobacillus rhamnosus of cfu/g, bacterium powder viable count are 1.0 * 10
10-5.2 * 10
10The lactobacillus reuteri of cfu/g, bacterium powder viable count are 1.0 * 10
10-4.0 * 10
10Cfu/g lactobacillus bulgaricus, bacterium powder viable count are 1.0 * 10
10-6.0 * 10
10One or more mixtures in the bifidobacterium bifidum of cfu/g, bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum, the blue and green Bifidobacterium.
The preparation of said probiotics bacterial mud is that lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus reuteri or lactobacillus bulgaricus are activated in the MRS fluid nutrient medium respectively, and microscopy, survey OD value, determine not have assorted bacterium, the OD value〉0.5 back the activation three times, again it is inserted the 10L fermentation tank, 37 ℃ of constant temperature culture 24h, stir once every 3-4h in the incubation, rotating speed 60 commentaries on classics/min, mixing time 2min obtains probiotics bacterial mud at 20 ℃ with the centrifugal 15min of 6000 commentaries on classics/min then.
Bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum or blue and green Bifidobacterium are added Bifidobacterium quality 0.05% respectively in the MRS fluid nutrient medium cysteine is got in the preparation of said probiotics bacterial mud, and microscopy, survey OD value〉it is inserted the 10L fermentation tank after the 0.5 back activation three times, and logical nitrogen is cultivated Bifidobacterium, and the cysteine salt of interpolation Bifidobacterium quality 0.05%, through 24h cultivate the back 20 ℃ with the centrifugal 15min of 6000 commentaries on classics/min, acquisition probiotics bacterial mud.
The effect of invention
1) the present invention is added on condensed skimmed or the interpolation of full-cream sheep Ruzhong with prebiotics, helps prebiotics and is uniformly dispersed;
2) probio is adopted the desivac preparation, and has added freeze drying protectant, the viable count height, and bacterial classification is 10
10More than the cfu/g;
3) by in goat milk powder, adding prebiotics, probio also there is protective effect, can improves the survival rate of probio.
The specific embodiment
Further specify the present invention below in conjunction with embodiment:
Embodiment 1
First: at first will make probiotics bacterial mud after the probio activation, in probiotics bacterial mud, add the compound freeze drying protectant of identical weight then, in-40 ℃ of pre-freeze 8h, move to freeze drier freeze drying 20-24h again and obtain freeze-drying probiotics bacterial powder behind the mixing;
Wherein, compound freeze drying protectant is by mass percentage by 30% skimmed milk power, and 20% trehalose, 2% glutamic acid, 1% glycerine and 47% pH are that 6.5 citrate buffer solution is formed;
The preparation of probiotics bacterial mud is to be 1.0 * 10 with bacterium powder viable count
10-2.0 * 10
11The lactobacillus acidophilus of cfu/g activates in the MRS fluid nutrient medium, and microscopy, survey OD value, determine not have assorted bacterium, the OD value〉0.5 back the activation three times, again it is inserted 10L fermentation tank, 37 ℃ of constant temperature culture 24h, stir once every 3-4h in the incubation, rotating speed 60 commentaries on classics/min, mixing time 2min obtains probiotics bacterial mud at 20 ℃ with the centrifugal 15min of 6000 commentaries on classics/min then.
Second: raw material sheep breast after purification and standardization, if the full-cream goat milk powder of preparation symphysis unit directly carried out for the 3rd step, if prepare symphysis unit degreasing goat milk powder, is heated to 36-39 ℃ with raw material sheep breast and carries out degreasing, with fat content control＜0.2%;
The 3rd: the second full-cream sheep breast that obtain of step or degreasing sheep breast reduced pressure to concentrate to be made solid content reach 42-50% to obtain raw material sheep breast;
Raw material sheep Ruzhong after concentrating obtains to contain the degreasing goat milk powder or the full-cream goat milk powder of prebiotics by spray-drying after adding prebiotics, and sieves behind the cool to room temperature; Degreasing goat milk powder that contains prebiotics after will sieving again or full-cream goat milk powder mix with the probiotics bacterial powder under 2-6 ℃, aseptic condition and obtain synbiotic goat milk powder;
Said prebiotics addition is by raw material sheep breast 1.5g/ liter;
Said probiotics bacterial powder addition is by goat milk powder 1.0g/kg.
Embodiment 2:
First: at first will make probiotics bacterial mud after the probio activation, in probiotics bacterial mud, add the compound freeze drying protectant of identical weight then, in-40 ℃ of pre-freeze 8h, move to freeze drier freeze drying 20-24h again and obtain freeze-drying probiotics bacterial powder behind the mixing;
Wherein, compound freeze drying protectant is by mass percentage by 70% skimmed milk power, and 8% trehalose, 1% glutamic acid, 1% glycerine and 20% pH are that 6.5 citrate buffer solution is formed;
The preparation of probiotics bacterial mud is to be 1.0 * 10 with bacterium powder viable count
10-5.5 * 10
10The Lactobacillus casei of cfu/g, bacterium powder viable count are 1.0 * 10
10-3.4 * 10
10The Lactobacillus plantarum of cfu/g activates in the MRS fluid nutrient medium respectively, and microscopy, survey OD value, determine not have assorted bacterium, the OD value〉0.5 back the activation three times, again it is inserted 10L fermentation tank, 37 ℃ of constant temperature culture 24h, stir once every 3-4h in the incubation, rotating speed 60 commentaries on classics/min, mixing time 2min obtains probiotics bacterial mud at 20 ℃ with the centrifugal 15min of 6000 commentaries on classics/min then.
Second: raw material sheep breast after purification and standardization, if the full-cream goat milk powder of preparation symphysis unit directly carried out for the 3rd step, if prepare symphysis unit degreasing goat milk powder, is heated to 36-39 ℃ with raw material sheep breast and carries out degreasing, with fat content control＜0.2%;
The 3rd: the second full-cream sheep breast that obtain of step or degreasing sheep breast reduced pressure to concentrate to be made solid content reach 42-50% to obtain raw material sheep breast;
Raw material sheep Ruzhong after concentrating obtains to contain the degreasing goat milk powder or the full-cream goat milk powder of prebiotics by spray-drying after adding prebiotics, and sieves behind the cool to room temperature; Degreasing goat milk powder that contains prebiotics after will sieving again or full-cream goat milk powder mix with the probiotics bacterial powder under 2-6 ℃, aseptic condition and obtain synbiotic goat milk powder;
Said prebiotics addition is by raw material sheep breast 2.5g/ liter;
Said probiotics bacterial powder addition is by goat milk powder 3g/kg.
Embodiment 3:
First: at first will make probiotics bacterial mud after the probio activation, in probiotics bacterial mud, add the compound freeze drying protectant of identical weight then, in-40 ℃ of pre-freeze 8h, move to freeze drier freeze drying 20-24h again and obtain freeze-drying probiotics bacterial powder behind the mixing;
Wherein, compound freeze drying protectant is by mass percentage by 50% skimmed milk power, and 10% trehalose, 1.5% glutamic acid, 1.5% glycerine and 37% pH are that 6.5 citrate buffer solution is formed;
The preparation of probiotics bacterial mud is to be 1.0 * 10 with bacterium powder viable count
10-5.6 * 10
10The Lactobacillus rhamnosus of cfu/g, bacterium powder viable count are 1.0 * 10
10-5.2 * 10
10The lactobacillus reuteri of cfu/g and bacterium powder viable count are 1.0 * 10
10-4.0 * 10
10The cfu/g lactobacillus bulgaricus activates in the MRS fluid nutrient medium respectively, and microscopy, survey OD value, determine not have assorted bacterium, the OD value〉0.5 back the activation three times, again it is inserted 10L fermentation tank, 37 ℃ of constant temperature culture 24h, stir once every 3-4h in the incubation, rotating speed 60 commentaries on classics/min, mixing time 2min obtains probiotics bacterial mud at 20 ℃ with the centrifugal 15min of 6000 commentaries on classics/min then.
Second: raw material sheep breast after purification and standardization, if the full-cream goat milk powder of preparation symphysis unit directly carried out for the 3rd step, if prepare symphysis unit degreasing goat milk powder, is heated to 36-39 ℃ with raw material sheep breast and carries out degreasing, with fat content control＜0.2%;
The 3rd: the second full-cream sheep breast that obtain of step or degreasing sheep breast reduced pressure to concentrate to be made solid content reach 42-50% to obtain raw material sheep breast;
Raw material sheep Ruzhong after concentrating obtains to contain the degreasing goat milk powder or the full-cream goat milk powder of prebiotics by spray-drying after adding prebiotics, and sieves behind the cool to room temperature; Degreasing goat milk powder that contains prebiotics after will sieving again or full-cream goat milk powder mix with the probiotics bacterial powder under 2-6 ℃, aseptic condition and obtain synbiotic goat milk powder;
Said prebiotics addition is by raw material sheep breast 4g/ liter;
Said probiotics bacterial powder addition is by goat milk powder 5g/kg.
Embodiment 4:
First: at first will make probiotics bacterial mud after the probio activation, in probiotics bacterial mud, add the compound freeze drying protectant of identical weight then, in-40 ℃ of pre-freeze 8h, move to freeze drier freeze drying 20-24h again and obtain freeze-drying probiotics bacterial powder behind the mixing;
Wherein, compound freeze drying protectant is by mass percentage by 40% skimmed milk power, and 15% trehalose, 1% glutamic acid, 2% glycerine and 42% pH are that 6.5 citrate buffer solution is formed;
It is 1.0 * 10 that bacterium powder viable count is adopted in the preparation of probiotics bacterial mud
10-6.0 * 10
10The bifidobacterium bifidum of cfu/g adds the cysteine of its quality 0.05% in the MRS fluid nutrient medium, and microscopy, survey OD value〉it is inserted the 10L fermentation tank after the 0.5 back activation three times, and logical nitrogen is cultivated Bifidobacterium, and the cysteine salt of interpolation Bifidobacterium quality 0.05%, through 24h cultivate the back 20 ℃ with the centrifugal 15min of 6000 commentaries on classics/min, acquisition probiotics bacterial mud.
Second: raw material sheep breast after purification and standardization, if the full-cream goat milk powder of preparation symphysis unit directly carried out for the 3rd step, if prepare symphysis unit degreasing goat milk powder, is heated to 36-39 ℃ with raw material sheep breast and carries out degreasing, with fat content control＜0.2%;
The 3rd: the second full-cream sheep breast that obtain of step or degreasing sheep breast reduced pressure to concentrate to be made solid content reach 42-50% to obtain raw material sheep breast;
Prebiotics is mixed under aseptic condition with the probiotics bacterial powder, cool to room temperature sieves and obtains degreasing goat milk powder or full-cream goat milk powder after raw material sheep after will concentrating the then breast spray-drying, mixed prebiotics and probiotics bacterial powder is directly joined in degreasing goat milk powder or the full-cream goat milk powder make synbiotic goat milk powder;
Said prebiotics addition is by raw material sheep breast 6.5g/ liter;
Said probiotics bacterial powder addition is by goat milk powder 10g/kg.
Embodiment 5:
First: at first will make probiotics bacterial mud after the probio activation, in probiotics bacterial mud, add the compound freeze drying protectant of identical weight then, in-40 ℃ of pre-freeze 8h, move to freeze drier freeze drying 20-24h again and obtain freeze-drying probiotics bacterial powder behind the mixing;
Wherein, compound freeze drying protectant is by mass percentage by 35% skimmed milk power, and 13% trehalose, 1% glutamic acid, 1% glycerine and 50% pH are that 6.5 citrate buffer solution is formed;
It is 1.0 * 10 that bacterium powder viable count is adopted in the preparation of probiotics bacterial mud
10-6.0 * 10
10The bifidobacterium infantis of cfu/g and bifidobacterium breve add the cysteine of its quality 0.05% respectively in the MRS fluid nutrient medium, and microscopy, survey OD value〉it is inserted the 10L fermentation tank after the 0.5 back activation three times, and logical nitrogen is cultivated Bifidobacterium, and the cysteine salt of interpolation Bifidobacterium quality 0.05%, through 24h cultivate the back 20 ℃ with the centrifugal 15min of 6000 commentaries on classics/min, acquisition probiotics bacterial mud.
Second: raw material sheep breast after purification and standardization, if the full-cream goat milk powder of preparation symphysis unit directly carried out for the 3rd step, if prepare symphysis unit degreasing goat milk powder, is heated to 36-39 ℃ with raw material sheep breast and carries out degreasing, with fat content control＜0.2%;
The 3rd: the second full-cream sheep breast that obtain of step or degreasing sheep breast reduced pressure to concentrate to be made solid content reach 42-50% to obtain raw material sheep breast;
Or prebiotics mixed under aseptic condition with the probiotics bacterial powder, cool to room temperature sieves and obtains degreasing goat milk powder or full-cream goat milk powder after raw material sheep after will concentrating the then breast spray-drying, mixed prebiotics and probiotics bacterial powder is directly joined in degreasing goat milk powder or the full-cream goat milk powder make synbiotic goat milk powder;
Said prebiotics addition is by raw material sheep breast 5g/ liter;
Said probiotics bacterial powder addition is by goat milk powder 8g/kg.
Scope of the present invention is not subjected to the restriction of described specific embodiments, and only as the single example of illustrating each side of the present invention, the scope of the invention also comprises the method and the component of functional equivalent to described embodiment.Those skilled in the art still can make various improvement and conversion to the present invention under the prerequisite that does not break away from the claim limited range, improvement of being done and conversion still belong to protection scope of the present invention.
Claims (5)
1. the preparation method of a synbiotic goat milk powder is characterized in that:
First: at first will make probiotics bacterial mud after the probio activation, in probiotics bacterial mud, add the compound freeze drying protectant of identical weight then, in-40 ℃ of pre-freeze 8-12h, move to freeze drier freeze drying 20-24h again and obtain freeze-drying probiotics bacterial powder behind the mixing;
Second: raw material sheep breast after purification and standardization, if the full-cream goat milk powder of preparation symphysis unit directly carried out for the 3rd step, if prepare symphysis unit degreasing goat milk powder, is heated to 36-39 ℃ with raw material sheep breast and carries out degreasing, with fat content control＜0.2%;
The 3rd: the second full-cream sheep breast that obtain of step or degreasing sheep breast reduced pressure to concentrate to be made solid content reach 42-50% to obtain raw material sheep breast;
Raw material sheep Ruzhong after concentrating obtains to contain the degreasing goat milk powder or the full-cream goat milk powder of prebiotics by spray-drying after adding prebiotics, and sieves behind the cool to room temperature; Degreasing goat milk powder that contains prebiotics after will sieving again or full-cream goat milk powder mix with the probiotics bacterial powder under 2-6 ℃, aseptic condition and obtain synbiotic goat milk powder;
Or prebiotics mixed under aseptic condition with the probiotics bacterial powder, cool to room temperature sieves and obtains degreasing goat milk powder or full-cream goat milk powder after raw material sheep after will concentrating the then breast spray-drying, mixed prebiotics and probiotics bacterial powder is directly joined in degreasing goat milk powder or the full-cream goat milk powder make synbiotic goat milk powder;
Said prebiotics addition is by raw material sheep breast 1.5g-6.5g/ liter;
Said probiotics bacterial powder addition is by goat milk powder 1.0-10g/kg.
2. the preparation method of synbiotic goat milk powder according to claim 1; it is characterized in that: said compound freeze drying protectant is by mass percentage by the skimmed milk power of 30%-70%; the trehalose of 8%-20%; the glutamic acid of 1%-2%, the glycerine of 1%-2% and the pH of 20-50% are 6.5 citrate buffer solution composition.
3. the preparation method of synbiotic goat milk powder according to claim 1, it is characterized in that: said probio is that bacterium powder viable count is 1.0 * 10
10-2.0 * 10
11The lactobacillus acidophilus of cfu/g, bacterium powder viable count are 1.0 * 10
10-5.5 * 10
10The Lactobacillus casei of cfu/g, bacterium powder viable count are 1.0 * 10
10-3.4 * 10
10The Lactobacillus plantarum of cfu/g, bacterium powder viable count are 1.0 * 10
10-5.6 * 10
10The Lactobacillus rhamnosus of cfu/g, bacterium powder viable count are 1.0 * 10
10-5.2 * 10
10The lactobacillus reuteri of cfu/g, bacterium powder viable count are 1.0 * 10
10-4.0 * 10
10Cfu/g lactobacillus bulgaricus, bacterium powder viable count are 1.0 * 10
10-6.0 * 10
10One or more mixtures in the bifidobacterium bifidum of cfu/g, bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum, the blue and green Bifidobacterium.
4. according to the preparation method of claim 1 or 3 described synbiotic goat milk powders, it is characterized in that: the preparation of said probiotics bacterial mud is with lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus reuteri or lactobacillus bulgaricus activate in the MRS fluid nutrient medium respectively, and microscopy, survey the OD value, determine not have assorted bacterium, the OD value〉0.5 back the activation three times, again it is inserted the 10L fermentation tank, 37 ℃ of constant temperature culture 24h, stir once every 3-4h in the incubation, rotating speed 60 commentaries on classics/min, mixing time 2min obtains probiotics bacterial mud at 20 ℃ with the centrifugal 15min of 6000 commentaries on classics/min then.
5. according to the preparation method of claim 1 or 3 described synbiotic goat milk powders, it is characterized in that: bifidobacterium bifidum is got in the preparation of said probiotics bacterial mud, bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum or blue and green Bifidobacterium are added the cysteine of Bifidobacterium quality 0.05% respectively in the MRS fluid nutrient medium, and microscopy, survey OD value〉after the 0.5 back activation three times with its access 10L fermentation tank, and logical nitrogen is cultivated Bifidobacterium, and the cysteine salt of interpolation Bifidobacterium quality 0.05%, through 24h cultivate the back 20 ℃ with the centrifugal 15min of 6000 commentaries on classics/min, acquisition probiotics bacterial mud.
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| CN109527097A (en) * | 2018-12-28 | 2019-03-29 | 陕西科技大学 | A kind of lactic acid bacteria goat milk tablet and preparation method thereof |
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| CN109527096A (en) * | 2018-12-28 | 2019-03-29 | 陕西科技大学 | A kind of probiotic formulation goat milk powder and preparation method thereof |
| CN113796421A (en) * | 2021-08-13 | 2021-12-17 | 盛锦合生物科技(江苏)有限公司 | Probiotic formula milk powder and preparation method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101199300A (en) * | 2007-12-17 | 2008-06-18 | 石家庄三鹿集团股份有限公司 | Yishengyuan powdered milk and preparation method thereof |
| CN101449830A (en) * | 2008-12-29 | 2009-06-10 | 山东龙力生物科技有限公司 | Mid-aged milk powder added with biostime and preparation method thereof |
| CN101449708A (en) * | 2008-12-29 | 2009-06-10 | 山东龙力生物科技有限公司 | Infant milk powder added with biostime and preparation method thereof |
-
2010
- 2010-04-23 CN CN2010101538992A patent/CN101810220B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101199300A (en) * | 2007-12-17 | 2008-06-18 | 石家庄三鹿集团股份有限公司 | Yishengyuan powdered milk and preparation method thereof |
| CN101449830A (en) * | 2008-12-29 | 2009-06-10 | 山东龙力生物科技有限公司 | Mid-aged milk powder added with biostime and preparation method thereof |
| CN101449708A (en) * | 2008-12-29 | 2009-06-10 | 山东龙力生物科技有限公司 | Infant milk powder added with biostime and preparation method thereof |
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| CN108935704A (en) * | 2018-07-16 | 2018-12-07 | 陕西科技大学 | A kind of probiotics goat milk powder and preparation method thereof |
| CN109527097A (en) * | 2018-12-28 | 2019-03-29 | 陕西科技大学 | A kind of lactic acid bacteria goat milk tablet and preparation method thereof |
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| CN109527096A (en) * | 2018-12-28 | 2019-03-29 | 陕西科技大学 | A kind of probiotic formulation goat milk powder and preparation method thereof |
| CN113796421A (en) * | 2021-08-13 | 2021-12-17 | 盛锦合生物科技(江苏)有限公司 | Probiotic formula milk powder and preparation method thereof |
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