CN103918795A - Tea polyphenols probiotics goat milk tablet and preparation method thereof - Google Patents
Tea polyphenols probiotics goat milk tablet and preparation method thereof Download PDFInfo
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Abstract
The invention provides a tea polyphenols probiotics goat milk tablet and a preparation method thereof. The tea polyphenols probiotics goat milk tablet comprises the following components in percentage by weight: 83.48%-90.19% of goat milk powder, 0.01-0.02% of tea polyphenols, 0.1%-1% of lyophilized probiotics powder, 5%-7% of sugar, 0.5% -1.0% of inulin, 4%-7% of glucose and 0.2%-0.5% of microcrystalline cellulose. The lyophilized probiotics powder is prepared from the mixture of probiotics and composite cryoprotectant blended according to the ratio of 1g:1mL. In the invention, polyphenols are added into the goat milk tablet which endows the milk tablet with the flavor of tea, also has the anti-oxidation effect, and improves the growth and reproduction and the survival rate of the probiotics. The absorption rate of the milk tablet is very high because the milk tablet contains short and medium chain unsaturated fatty acid which can be directly used by a human body. The EGF in the goat milk can promote the growth of epithelial cells and repair the mucosal epithelial cells of stomach, trachea and skin.
Description
Technical field:
The invention belongs to field of health care food, be specifically related to a kind of Tea Polyphenols probio goat milk sheet and preparation method thereof.
Background technology:
Goat milk, with high nutrition, high-quality health idea, strides greatly and has come into consumer's sight line, has met national consumption hobby and nutritional need, becomes the another high consumption focus outside milk.Especially the high-quality goat milk that Shaanxi produces, be subject to especially the height approval of domestic market, and more and more goat milk brand starts to go abroad, and explores overseas market actively, fully demonstrates the potentiality that should not be underestimated in goat milk market.
Goat Milk has been widely regarded as and has been particularly suitable for the food that the elderly, sick people, sick convalescent people and infant use.Goat milk is existing has many advantages for milk: 1. Goat Milk medium chain fatty acid content is high, and wherein aliphatic acid is divided into three kinds, long, medium and short chain.Medium chain and SCFA are more easily subject to the attack of body fat enzyme and digested absorption than long-chain.In addition, medium chain fatty acid has unique mechanism of absorption, can at small intestine, directly be absorbed without the digestion of pancreas enzyme and cholate, and the content of Goat Milk medium chain fatty acid is the twice of milk.2. Goat Milk fat globule diameter is little.The diameter of fat globule is less, just has larger surface area to attack for human intestine's lipase, makes fat be decomposed faster.In general, the digestion of Goat Milk is than milk fast 20%.Although milk and the Goat Milk separately diameter of fat globule have difference,, on average, the diameter of Goat Milk fat globule is more much smaller than milk.General upper, the diameter of Goat Milk fat globule is 2-2.5 micron, and milk is 3-4.25 micron.The digestion process of little, the frangible protein of curdled milk hardness that Goat Milk forms in human body is first under hydrochloric acid in gastric juice effect, to form curdled milk, and the digestion that is then decomposed, for amino acid, is absorbed at small intestine afterwards.The soft durometer of ziega directly affects the digested speed of protein and efficiency.In Goat Milk, main protein is beta-casein, has structural difference with protein alpha-S1 casein main in milk.It is soft more frangible than milk that the ziega that Goat Milk beta-casein forms has under one's belt been proved to be.3. strengthen the absorption of iron and calcium.Iron in milk and calcium, owing to there being interaction, can have influence on wherein a kind of absorption of element.And iron and calcium in Goat Milk does not have this interaction, so bioavilability is higher than milk.
Probio (Probiotics), claim again probiotic, regulator, raw rhzomorph, viable bacteria element etc., refer to certain microorganism formulation or fermented product, by regulating host's mucous membrane and systemic immune function and improving enteral nutrition and colony balance, host is produced to useful physiological action, by colonized action, change the composition of a certain position of host flora, thereby produce, be conducive to the single of host health effect or form clear and definite mixed microorganism.Up to now, the probio that scientist has found can be divided into three major types substantially, comprising: 1. lactobacillus class (as lactobacillus acidophilus, Lactobacillus casei, lactobacillus bulgaricus, lactobacillus plantarum etc.); 2. Bifidobacterium class (as bifidobacterium longum, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis etc.); 3. gram-positive cocci (as streptococcus fecalis, galactococcus, intermediary streptococcus etc.).Probio is as a frontier, and in recent years, the special physiological activity of probio is widely used in food, except being applied to fermented dairy product, is also just progressively applied to developing various functional foods.At present, probio is mainly used in the fields such as food, functional food, dietary supplements, medicinal organism goods and feed.Being referred to as probiotic food containing the food of profitable probliotics, mainly comprise the food of direct interpolation probio and through the food of probiotics fermention.Probio the most general application in field of food is still dairy products field, comprises acidified milk, active lactic acid bacteria drink, cheese, sour cream, kefyr, fruit juice, cereal preparation, biscuit etc.With probio bacterial classification have Bifidobacterium (Bifidobateria), Bacillus acidi lactici and some streptococcus, wherein, Bacillus acidi lactici is conventional lactobacillus acidophilus (Lactobicullus acidophilus), Lactobacillus casei (L.casei), lactobacillus bulgaricus (L.bulgaricus) etc.The probio bacterial classification that can be used for health food of China Ministry of Public Health approval at present has: lactobacillus acidophilus, Lactobacillus casei, lactobacillus reuteri, Lactobacillus rhamnosus, bifidobacterium bifidum, bifidobacterium infantis, bifidobacterium breve, bifidobacterium longum, blue and green Bifidobacterium, lactobacillus bulgaricus, streptococcus thermophilus etc.
Tea Polyphenols, claims again tea to tan or tea tannin, is pale yellow or shallow green powder under normal temperature, has no side effect, and free from extraneous odour, is the general name of Polyphenols of Tea, comprises flavanol compound, anthocyanin class, flavonoids, flavonols and phenolic acid class etc.Wherein important with flavanol compound material.
Large quantity research shows, the effects such as that Tea Polyphenols isoreactivity material has is anti-oxidant, build up resistance, removing toxic substances, hypoglycemic, reducing blood lipid, angiocardiopathy preventing, anti-ageing, antitumor, radioresistance, are described as by health and medical circle " radiation jinx ".1989 Nian Bei Food Additives Used in China associations list GB2760-89 food additives in and use standard, within 1997, classify Chinese patent drug raw material as.
Microcrystalline cellulose is the cellulose of a kind of purifying, part depolymerization, white, odorless, tasteless, the crystalline powder being comprised of small porous particle.Be widely used in the industries such as pharmacy, cosmetics, food, not only can be used for wet granulation and also can be used for dry method direct tablet compressing.In tablet preparation, can be used as disintegrant and adhesive, these two kinds of effects are integrated, and consumption is few, safety, effectively avoided multiple additives to use the harmful effect causing simultaneously.
In recent years, the research great majority of Tea Polyphenols are for tea beverage industry, and patent (CN 103098923A) has been announced a kind of instant tea method for preparing beverage, patent (CN 101558804A) announced tea beverage manufacture method; Aspect tablet, slightly report, patent (CN 101444502A) has been announced a kind of preparation method of high-purity tea polyphenol buccal tablets, and patent (CN 102188543A) has been announced a kind of preparation method of tea polyphenol tablet.But the report that is applied to probio aspect as the prebiotic factor for Tea Polyphenols is very few, and application microcrystalline cellulose is also less as the relevant report of adhesive and this double action of disintegrant, and goat milk is compared with milk and has many advantages simultaneously.Therefore the material that has added probio and Tea Polyphenols in goat milk sheet can improve enteral nutrition and colony balance effectively, be conducive to the Growth and reproduction of probio, and improve its survival rate, guaranteed the quantity of probio in milk sheet, the nutritious safety of milk sheet obtaining, easy to carry.
Summary of the invention:
For above-mentioned defect or deficiency, the invention provides a kind of Tea Polyphenols probio goat milk sheet and preparation method thereof, prepared goat milk sheet can improve enteral nutrition and colony balance effectively.
For reaching above object, technical scheme of the present invention is:
A kind of Tea Polyphenols probio goat milk sheet, the goat milk powder that comprises by mass percentage 83.48%-90.19%, freeze-drying probiotics bacterial powder, the sucrose of 5%-7%, the glucose of the synanthrin of 0.5%-1.0%, 4%-7% and the microcrystalline cellulose of 0.2%-0.5% of the Tea Polyphenols of 0.01%-0.02%, 0.1%-1%.
Described freeze-drying probiotics bacterial powder is the mixture of probio and compound freeze drying protectant, and probio mixes with the ratio of the compound freeze drying protectant of 1mL according to every g probio with compound freeze drying protectant.
It is (1.0-3.0) * 10 that described probio adopts viable count
11lactobacillus acidophilus bacterium powder and viable count be (1.0-3.0) * 10
11bifidobacterium bifidum bacterium powder with arbitrary proportion, mix.
Described compound freeze drying protectant comprises by mass percentage: the skimmed milk power of 8%-50%, the trehalose of 8%-20%, the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer.
A preparation method for Tea Polyphenols probio goat milk sheet, comprises the following steps:
1) probio of getting after activation is accessed the fermentation tank culture of MRS culture medium with 2～5% inoculum concentration, in incubation, every 3-4h, stir once, then, centrifugal with centrifuge, make probiotics bacterial mud, then in probiotics bacterial mud, in the ratio of the compound freeze drying protectant of every g probiotics bacterial mud interpolation 1mL, add compound freeze drying protectant, after mixing, pre-freeze 12～24h at-40～-20 ℃, then carry out freeze drying, obtain freeze-drying probiotics bacterial powder;
2) by sheep breast through purify and standardization after, reduced pressure concentration makes solid content reach 42～50% and obtains raw material sheep breast, by crossing 40～80 mesh sieves after spray dry and cool to room temperature, obtains goat milk powder;
3) by mass percentage by the goat milk powder of 83.48%-90.19%, freeze-drying probiotics bacterial powder, the sucrose of 5%-7%, the glucose of the synanthrin of 0.5%-1.0%, 4%-7% and the microcrystalline cellulose of 0.2%-0.5% of the Tea Polyphenols of 0.01%-0.02%, 0.1%-1% mix, and obtain Tea Polyphenols probio goat milk powder;
4) utilize tablet press machine, the Tea Polyphenols probio goat milk powder unit of making viable count is reached to (1.0-3.0) * 10
7～(1.0-3.0) * 10
9the probio goat milk sheet of cfu/g.
Described step 2) dry employing blast drier is dry at 70 ℃.
Described probio is that viable count is 1.0-3.0 * 10
11lactobacillus acidophilus bacterium powder, viable count be 1.0-3.0 * 10
11the mixing of these two kinds of arbitrary proportions of Bifidobacteria powder.
Described compound freeze drying protectant is by mass percentage by the skimmed milk power of 18%-50%, the trehalose of 8%-20%, and the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer form.
The dry EAT of described spraying is 160～165 ℃, and temperature of outgoing air is 75～65 ℃.
In described step 1), mixing employing rotating speed is 5～20 turn/min, the three-dimensional motion mixer that power of motor is 0.5Kw; The tablet press machine of described step 4) adopts single-punching tablet press, the maximum compressing tablet pressure of single-punching tablet press: 50KN; Compressing tablet diameter:
10～18mm, sheet is heavily 1.0～1.6g; Hardness is at 4～7N.
For reaching above object, beneficial effect of the present invention is:
1) in the present invention, main component is goat milk powder, because Goat Milk is rich in high-quality protein and aliphatic acid and the several mineral materials such as albumin that health is played an important role, globulin, to nutrient absorption, bad syndrome patient has good health care and therapeutic action.Goat milk fat globule little (be only milk 1/3), and be rich in be beneficial to that body directly utilizes in, short chain unrighted acid, absorptivity is very high.The EGF(EGF containing in goat milk), when promoting epithelial cell growth, the mucomembranous epithelial cells such as stomach, tracheae and skin are had to repair, can urge digestion again can tranquilizing and allaying excitement (because containing cyclic nucleotide), and can strengthen immunity of organisms, and regulate human body nutrient balance, improve sleep quality, the comfort cushioning characteristic of its meta-alkalescence also has the effect that promotes rehabilitation to diseases such as ephritis, tuberculosis and asthma, be infant, pregnant woman, old man and patient's excellent health products.
2) added in the present invention probio, probio has obvious facilitation to health, science proves that it has following effect: 1, prevent and improve constipation, diarrhoea, safeguard enteron aisle normal bacteria colony balance, suppress the growth of pathogen, pass through bidirectional modulation, prevent constipation, diarrhoea, adjusts function of intestinal canal disorderly, prevents various intestines problems.2, antitumor, from the isolated antitumor polysaccharide of Bifidobacterium, enzyme is lost activity, thus the generation of pre-anti-cancer.3, control the cholesterol in serum, a large amount of clinical trial both at home and abroad confirm, take lactobacillary milk goods and have the human serum of minimizing cholesterol level, reduce the effect of cardiovascular disease incidence rate.1997, Gimland has proposed lactic acid bacteria at first can promote by degraded cholate the catabolism of cholesterol, thereby reduced the viewpoint of cholesterol level.4, biological barrier effect, reduces the generation of harmful substance in harmful bacteria metabolin, forms the biomembrane barrier that one deck has protective effect, stops the invasion of pernicious bacteria, accelerates the discharge of various toxin in enteron aisle.Play the effect that improves intestinal environment and protection enteron aisle.5, promote generation and the absorption of nutriment in enteron aisle, synthetic vitamin B can self be synthesized or promote to Bifidobacterium
1, B
2, B
6, B
12and the nutriment of the needed by human such as amino acid., improve the absorption to calcium ion, realize stomach normalization.6, row's stool, the fat that purifies the blood, hypotensive, accelerate lipolysis, old loose fat in adsorbent, reduces serum cholesterol level.Thereby hypertension and artery sclerosis are had to certain prevention effect.7, improve immunity, there is the cytotostatic of promotion and increase intestine immunity enhanced feature, prevent antibiotic side effect, trigger cell mutation factor is had to resistant function.Anti-ageing, promote longevity.8, improve lactose intolerance, the ferment that Bifidobacterium reduces lactose, to help improve lactose intolerance.9, other functions, as food hypersenstivity, prevention diabetes etc.Exactly because probio, for the effect of human health, receives much concern it.
3) added in the present invention Tea Polyphenols, not only give milk sheet peculiar tea smell, but also there is antioxidation, it can improve the Growth and reproduction of probio, improve its survival rate, mainly due to the probio relating in the present invention, belong to anaerobic bacterial strain, when aerobic exists, death can occur, and at this moment Tea Polyphenols has oxidation resistant function, can with combination with oxygen, avoided the combination of oxygen and probio, thereby the death rate that reduces probio, has improved its survival rate, has guaranteed the quantity of probio.
The specific embodiment:
Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Embodiment 1:
1) probio is adopted under 37 ℃ of condition of culture the activation of MRS fluid nutrient medium obtain activating probio three times, the probio of getting after activation is accessed the fermentation tank culture 18h of 10L MRS culture medium with 2% inoculum concentration, in incubation, every 3h, stir once, turn/min of rotating speed 60, mixing time 2min, then, with centrifuge, with the centrifugal 12min of rotating speed of 10000 turn/min, make probiotics bacterial mud, then in probiotics bacterial mud, press the compound freeze drying protectant that every g probiotics bacterial mud adds 1mL, utilize vortex mixed instrument to be mixed even, at-40 ℃ of pre-freeze 12h, move to again freeze drier freeze drying 24h and obtain freeze-drying probiotics bacterial powder,
Described compound freeze drying protectant is by mass percentage by 18% skimmed milk power, 20% trehalose, and 2% sodium hydrogen phosphate, 10% lactose and 50% phosphate buffer form;
Wherein, the activation process of probio specifically comprises:
Lactobacillus acidophilus is adopted under 37 ℃ of condition of culture to MRS fluid nutrient medium activation three times, the cysteine hydrochloride that Bifidobacterium is added culture volume 0.05% in MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture, with 5% inoculum concentration, transfer at every turn, wherein all under 37 ℃ of condition of culture, cultivate 20-24h first twice, under 37 ℃ of condition of culture, cultivate 18h for the third time, then carry out microscopy, survey OD value, determine without activating for the third time and obtain activating probio after miscellaneous bacteria.
2) make solid content reach 42% raw material sheep breast reduced pressure concentration after purification and standardization and obtain raw material sheep breast, after raw material sheep breast is dried (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) by spraying, cool to room temperature is crossed the sub goat milk powder that obtains of 40 mesh sieves;
3) by mass percentage by 83.48% goat milk powder, it is 5 turn/min that 0.02% Tea Polyphenols, 1% freeze-drying probiotics bacterial powder, 7% sucrose, 1.0% synanthrin, 7% glucose and 0.5% microcrystalline cellulose adopt rotating speed, and the three-dimensional motion mixer that power of motor is 0.5Kw mixes;
Then utilize single-punching tablet press, the unit's of making viable count reaches (1.0-3.0) * 10
7～(1.0-3.0) * 10
9the probio goat milk sheet containing Tea Polyphenols of cfu/g.
The maximum compressing tablet pressure of described tablet press machine: 50KN; Compressing tablet diameter:
10mm; Sheet is heavily 1.0g; Hardness is at 4～7N.
Embodiment 2:
1) probio is adopted under 37 ℃ of condition of culture the activation of MRS fluid nutrient medium obtain activating probio three times, the probio of getting after activation is accessed the fermentation tank culture 18h of 10L MRS culture medium with 3% inoculum concentration, in incubation, every 3h, stir once, turn/min of rotating speed 60, mixing time 2min, then, with centrifuge, with the centrifugal 12min of rotating speed of 10000 turn/min, make probiotics bacterial mud, then in probiotics bacterial mud, press the compound freeze drying protectant that every g probiotics bacterial mud adds 1mL, utilize vortex mixed instrument to be mixed even, at-40 ℃ of pre-freeze 12h, move to again freeze drier freeze drying 24h and obtain freeze-drying probiotics bacterial powder,
Described compound freeze drying protectant is by mass percentage by 18% skimmed milk power, 20% trehalose, and 2% sodium hydrogen phosphate, 10% lactose and 50% phosphate buffer form;
Wherein, the activation process of probio specifically comprises:
Lactobacillus acidophilus is adopted under 37 ℃ of condition of culture to MRS fluid nutrient medium activation three times, the cysteine hydrochloride that Bifidobacterium is added culture volume 0.05% in MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture, with 5% inoculum concentration, transfer at every turn, wherein all under 37 ℃ of condition of culture, cultivate 20-24h first twice, under 37 ℃ of condition of culture, cultivate 18h for the third time, then carry out microscopy, survey OD value, determine without activating for the third time and obtain activating probio after miscellaneous bacteria.
2) make solid content reach 42% raw material sheep breast reduced pressure concentration after purification and standardization and obtain raw material sheep breast, after raw material sheep breast is dried (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) by spraying, cool to room temperature is crossed the sub goat milk powder that obtains of 60 mesh sieves;
3) by mass percentage by 90.19% goat milk powder, it is 5 turn/min that 0.01% Tea Polyphenols, 0.1% freeze-drying probiotics bacterial powder, 5% sucrose, 0.5% synanthrin, 4% glucose and 0.2% microcrystalline cellulose adopt rotating speed, and the three-dimensional motion mixer that power of motor is 0.5Kw mixes;
Then utilize single-punching tablet press, the unit's of making viable count reaches (1.0-3.0) * 10
7～(1.0-3.0) * 10
9the probio goat milk sheet containing Tea Polyphenols of cfu/g.
The maximum compressing tablet pressure of described tablet press machine: 15KN; Compressing tablet diameter:
14mm; Sheet is heavily 1.4g; Hardness is at 4～7N.
Embodiment 3:
1) probio is adopted under 37 ℃ of condition of culture the activation of MRS fluid nutrient medium obtain activating probio three times, the probio of getting after activation is accessed the fermentation tank culture 18h of 10L MRS culture medium with 4% inoculum concentration, in incubation, every 3h, stir once, turn/min of rotating speed 60, mixing time 2min, then, with centrifuge, with the centrifugal 12min of rotating speed of 10000 turn/min, make probiotics bacterial mud, then in probiotics bacterial mud, press the compound freeze drying protectant that every g probiotics bacterial mud adds 1mL, utilize vortex mixed instrument to be mixed even, at-40 ℃ of pre-freeze 12h, move to again freeze drier freeze drying 24h and obtain freeze-drying probiotics bacterial powder,
Described compound freeze drying protectant is by mass percentage by 18% skimmed milk power, 20% trehalose, and 2% sodium hydrogen phosphate, 10% lactose and 50% phosphate buffer form;
Wherein, the activation process of probio specifically comprises:
Lactobacillus acidophilus is adopted under 37 ℃ of condition of culture to MRS fluid nutrient medium activation three times, the cysteine hydrochloride that Bifidobacterium is added culture volume 0.05% in MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture, with 5% inoculum concentration, transfer at every turn, wherein all under 37 ℃ of condition of culture, cultivate 20-24h first twice, under 37 ℃ of condition of culture, cultivate 18h for the third time, then carry out microscopy, survey OD value, determine without activating for the third time and obtain activating probio after miscellaneous bacteria.
2) make solid content reach 42% raw material sheep breast reduced pressure concentration after purification and standardization and obtain raw material sheep breast, after raw material sheep breast is dried (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) by spraying, cool to room temperature is crossed the sub goat milk powder that obtains of 80 mesh sieves;
3) by mass percentage by 86.185% goat milk powder, it is 5 turn/min that 0.015% Tea Polyphenols, 0.5% freeze-drying probiotics bacterial powder, 6% sucrose, 1% synanthrin, 6% glucose and 0.3% microcrystalline cellulose adopt rotating speed, and the three-dimensional motion mixer that power of motor is 0.5Kw mixes;
Then utilize single-punching tablet press, the unit's of making viable count reaches (1.0-3.0) * 10
7～(1.0-3.0) * 10
9the probio goat milk sheet containing Tea Polyphenols of cfu/g.
The maximum compressing tablet pressure of described tablet press machine: 50KN; Compressing tablet diameter:
13mm; Sheet is heavily 1.5g; Hardness is at 4～7N.
Embodiment 4:
1) probio is adopted under 37 ℃ of condition of culture the activation of MRS fluid nutrient medium obtain activating probio three times, the probio of getting after activation is accessed the fermentation tank culture 18h of 10L MRS culture medium with 5% inoculum concentration, in incubation, every 3h, stir once, turn/min of rotating speed 60, mixing time 2min, then, with centrifuge, with the centrifugal 12min of rotating speed of 10000 turn/min, make probiotics bacterial mud, then in probiotics bacterial mud, press the compound freeze drying protectant that every g probiotics bacterial mud adds 1mL, utilize vortex mixed instrument to be mixed even, at-40 ℃ of pre-freeze 12h, move to again freeze drier freeze drying 24h and obtain freeze-drying probiotics bacterial powder,
Described compound freeze drying protectant is by mass percentage by 18% skimmed milk power, 20% trehalose, and 2% sodium hydrogen phosphate, 10% lactose and 50% phosphate buffer form;
Wherein, the activation process of probio specifically comprises:
Lactobacillus acidophilus is adopted under 37 ℃ of condition of culture to MRS fluid nutrient medium activation three times, the cysteine hydrochloride that Bifidobacterium is added culture volume 0.05% in MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture, with 5% inoculum concentration, transfer at every turn, wherein all under 37 ℃ of condition of culture, cultivate 20-24h first twice, under 37 ℃ of condition of culture, cultivate 18h for the third time, then carry out microscopy, survey OD value, determine without activating for the third time and obtain activating probio after miscellaneous bacteria.
2) make solid content reach 42% raw material sheep breast reduced pressure concentration after purification and standardization and obtain raw material sheep breast, after raw material sheep breast is dried (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) by spraying, cool to room temperature is crossed the sub goat milk powder that obtains of 50 mesh sieves;
3) by mass percentage by 85.48% goat milk powder, it is 5 turn/min that 0.02% Tea Polyphenols, 1% freeze-drying probiotics bacterial powder, 6% sucrose, 1.0% synanthrin, 6% glucose and 0.5% microcrystalline cellulose adopt rotating speed, and the three-dimensional motion mixer that power of motor is 0.5Kw mixes;
Then utilize single-punching tablet press, the unit's of making viable count reaches 1.0-3.0 * 10
7～1.0-3.0 * 10
9the probio goat milk sheet containing Tea Polyphenols of cfu/g.
The maximum compressing tablet pressure of described tablet press machine: 15KN; Compressing tablet diameter:
16mm; Sheet is heavily 1.5g; Hardness is at 4～7N.
Embodiment 5:
1) probio is adopted under 37 ℃ of condition of culture the activation of MRS fluid nutrient medium obtain activating probio three times, the probio of getting after activation is accessed the fermentation tank culture 18h of 10L MRS culture medium with 5% inoculum concentration, in incubation, every 3h, stir once, turn/min of rotating speed 60, mixing time 2min, then, with centrifuge, with the centrifugal 12min of rotating speed of 10000 turn/min, make probiotics bacterial mud, then in probiotics bacterial mud, press the compound freeze drying protectant that every g probiotics bacterial mud adds 1mL, utilize vortex mixed instrument to be mixed even, at-40 ℃ of pre-freeze 12h, move to again freeze drier freeze drying 24h and obtain freeze-drying probiotics bacterial powder,
Described compound freeze drying protectant is by mass percentage by 18% skimmed milk power, 20% trehalose, and 2% sodium hydrogen phosphate, 10% lactose and 50% phosphate buffer form;
Wherein, the activation process of probio specifically comprises:
Lactobacillus acidophilus is adopted under 37 ℃ of condition of culture to MRS fluid nutrient medium activation three times, the cysteine hydrochloride that Bifidobacterium is added culture volume 0.05% in MRS fluid nutrient medium adopts MRS fluid nutrient medium activation three times under 37 ℃ of condition of culture, with 5% inoculum concentration, transfer at every turn, wherein all under 37 ℃ of condition of culture, cultivate 20-24h first twice, under 37 ℃ of condition of culture, cultivate 18h for the third time, then carry out microscopy, survey OD value, determine without activating for the third time and obtain activating probio after miscellaneous bacteria.
2) make solid content reach 42% raw material sheep breast reduced pressure concentration after purification and standardization and obtain raw material sheep breast, after raw material sheep breast is dried (EAT is 160～165 ℃, and temperature of outgoing air is 75～65 ℃) by spraying, cool to room temperature is crossed the sub goat milk powder that obtains of 70 mesh sieves;
3) by mass percentage by 86.98% goat milk powder, it is 5 turn/min that 0.02% Tea Polyphenols, 0.5% freeze-drying probiotics bacterial powder, 6% sucrose, 1.0% synanthrin, 5% glucose and 0.5% microcrystalline cellulose adopt rotating speed, and the three-dimensional motion mixer that power of motor is 0.5Kw mixes;
Then utilize single-punching tablet press, the unit's of making viable count reaches (1.0-3.0) * 10
7～(1.0-3.0) * 10
9cfu/g is containing the probio goat milk sheet of Tea Polyphenols.
The maximum compressing tablet pressure of described tablet press machine: 15KN; Compressing tablet diameter:
18mm; Sheet is heavily 1.6g; Hardness is at 4～7N.
Claims (10)
1. a Tea Polyphenols probio goat milk sheet, it is characterized in that, the goat milk powder that comprises by mass percentage 83.48%-90.19%, freeze-drying probiotics bacterial powder, the sucrose of 5%-7%, the glucose of the synanthrin of 0.5%-1.0%, 4%-7% and the microcrystalline cellulose of 0.2%-0.5% of the Tea Polyphenols of 0.01%-0.02%, 0.1%-1%.
2. Tea Polyphenols probio goat milk sheet according to claim 1; it is characterized in that; described freeze-drying probiotics bacterial powder is the mixture of probio and compound freeze drying protectant, and probio mixes with the ratio of the compound freeze drying protectant of 1mL according to every g probio with compound freeze drying protectant.
3. Tea Polyphenols probio goat milk sheet according to claim 2, is characterized in that, it is (1.0-3.0) * 10 that described probio adopts viable count
11lactobacillus acidophilus bacterium powder and viable count be (1.0-3.0) * 10
11bifidobacterium bifidum bacterium powder with arbitrary proportion, mix.
4. according to the Tea Polyphenols probio goat milk sheet described in claim 2 or 3; it is characterized in that, described compound freeze drying protectant comprises by mass percentage: the skimmed milk power of 8%-50%, the trehalose of 8%-20%; the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer.
5. a preparation method for Tea Polyphenols probio goat milk sheet, is characterized in that, comprises the following steps:
1) probio of getting after activation is accessed the fermentation tank culture of MRS culture medium with 2～5% inoculum concentration, in incubation, every 3-4h, stir once, then, centrifugal with centrifuge, make probiotics bacterial mud, then in probiotics bacterial mud, in the ratio of the compound freeze drying protectant of every g probiotics bacterial mud interpolation 1mL, add compound freeze drying protectant, after mixing, pre-freeze 12～24h at-40～-20 ℃, then carry out freeze drying, obtain freeze-drying probiotics bacterial powder;
2) by sheep breast through purify and standardization after, reduced pressure concentration makes solid content reach 42～50% and obtains raw material sheep breast, by crossing 40～80 mesh sieves after spray dry and cool to room temperature, obtains goat milk powder;
3) by mass percentage by the goat milk powder of 83.48%-90.19%, freeze-drying probiotics bacterial powder, the sucrose of 5%-7%, the glucose of the synanthrin of 0.5%-1.0%, 4%-7% and the microcrystalline cellulose of 0.2%-0.5% of the Tea Polyphenols of 0.01%-0.02%, 0.1%-1% mix, and obtain Tea Polyphenols probio goat milk powder;
4) utilize tablet press machine, the Tea Polyphenols probio goat milk powder unit of making viable count is reached to (1.0-3.0) * 10
7～(1.0-3.0) * 10
9the probio goat milk sheet of cfu/g.
6. the preparation method of Tea Polyphenols probio goat milk sheet according to claim 5, is characterized in that described step 2) dry employing blast drier dry at 70 ℃.
7. the preparation method of Tea Polyphenols probio goat milk sheet according to claim 5, is characterized in that, described probio is that viable count is (1.0-3.0) * 10
11lactobacillus acidophilus bacterium powder, viable count be (1.0-3.0) * 10
11the mixing of these two kinds of arbitrary proportions of Bifidobacteria powder.
8. the preparation method of Tea Polyphenols probio goat milk sheet according to claim 5; it is characterized in that; described compound freeze drying protectant is by mass percentage by the skimmed milk power of 18%-50%; the trehalose of 8%-20%; the sodium hydrogen phosphate of 1%-2%, the lactose of 1%-10% and 40%-50% phosphate buffer form.
9. the preparation method of Tea Polyphenols probio goat milk sheet according to claim 5, is characterized in that, the dry EAT of described spraying is 160～165 ℃, and temperature of outgoing air is 75～65 ℃.
10. the preparation method of Tea Polyphenols probio goat milk sheet according to claim 5, is characterized in that, in described step 1), mixing employing rotating speed is 5～20 turn/min, the three-dimensional motion mixer that power of motor is 0.5Kw; The tablet press machine of described step 4) adopts single-punching tablet press, the maximum compressing tablet pressure of single-punching tablet press: 50KN; Compressing tablet diameter:
10～18mm, sheet is heavily 1.0～1.6g; Hardness is at 4～7N.
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| CN111743158A (en) * | 2020-06-08 | 2020-10-09 | 杭州娃哈哈科技有限公司 | Probiotic tablet with function of enhancing immunity and preparation method thereof |
| CN113575961A (en) * | 2021-07-30 | 2021-11-02 | 宁波大学 | Tea polyphenol probiotic lactic acid bacteria powder and preparation method thereof |
| CN116686987A (en) * | 2023-06-27 | 2023-09-05 | 温州医科大学附属第一医院 | Synbiotic health food containing green tea polyphenols extract and its preparation method |
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