CN101711831A - Pediatric Chinese medicinal oral liquid for preventing and treating H1N1 virus and preparation method thereof - Google Patents
Pediatric Chinese medicinal oral liquid for preventing and treating H1N1 virus and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a pediatric Chinese medicinal oral liquid for preventing and treating H1N1 virus and a preparation method thereof, relates to a pediatric medicament for preventing and treating the H1N1 virus and a preparation method thereof, and solves the problem that the conventional medicaments for preventing and treating the H1N1 virus are not used for children and have poor treatment effect and side effect. The pediatric Chinese medicinal oral liquid of the invention is prepared by adding water into ephedra herb, bitter apricot seed, gypsum, licorice root, honeysuckle flower, weeping forsythiae capsule, common anemarrhena rhizome, baikal skullcap root, indigowoad root, dwarf lilyturf turber, heartleaf houttuynia herb, preservative, and flavoring agent. The preparation method comprises the following steps: (1) preparing mixed decoction solution; (2) preparing mixed solution A; and (3) filtering the mixed solution A, concentrating the filtrate and adding the water into dilute the filtrate, stirring the mixture, performing cold storage treatment on the obtained product, then refiltering the obtained product, adding the preservative and the flavoring agent into the filtrate, and then adding the water, stirring the product and making volume constant to obtain the pediatric Chinese medicinal oral liquid for preventing and treating the H1N1 virus. The Chinese medicinal oral liquid of the invention has the effects of allaying asthma and relieving cough, relieving fever, eliminating phlegm, resisting allergy and resisting the H1N1 flu; and the product of the invention is safe and has no side or toxic effect.
Description
Technical field
The present invention relates to a kind of department of pediatrics medication that prevents and treats H1N1 virus and preparation method thereof.
Background technology
Entered since winter in 2009, the morbidity crowd of influenza A H1N1 has been developed into based on 6～20 years old age bracket, and child and teenager are just becoming infection and ill main colony, and still presents lasting trend occurred frequently.The child has the special body constitution of " YIN often being in shortage, YANG often being in excess ", and the core pathogenesis of the light disease of child's influenza A H1N1 is that wind heat epidemic disease drug-pedlar and lung are defended, and is main clinical manifestation with heating, cough, pharyngalgia etc.In " people infects influenza A H1N1 diagnosis and treatment scheme " of the approval of 2009 Ministry of Public Health, do not specify the diagnosis and treatment scheme that child and teenager colony suffer from the H1N1 influenza.According to expert introduction, the most medicines of China do not have children taking dosage, and it is suitable decrement under physician guidance that the normal children uses adult's medicine.Still in growth, the organ internal organs are tender and lovely for child and teenager, and some adult's medicine or component are not suitable for children taking, and therapeutic effect difference and side effect is arranged.
Summary of the invention
The present invention for solve existing control H1N1 virus the medicine needleless to children, and have the therapeutic effect difference and the problem of side effect arranged, and provide a kind of department of pediatrics of preventing and treating H1N1 virus with Chinese medicine oral liquid and preparation method thereof.
The department of pediatrics of control H1N1 virus is made by the water of correctives adding 800～1200mL of the antiseptic of the Herba Houttuyniae of Radix Ophiopogonis of the Radix Isatidis of the Radix Scutellariae of the Rhizoma Anemarrhenae of the Fructus Forsythiae of the Flos Lonicerae of the Radix Glycyrrhizae of the Gypsum Fibrosum of the Semen Armeniacae Amarum of the Herba Ephedrae of 40～60g, 90～110g, 350～450g, 40～60g, 160～170g, 160～170g, 160～170g, 160～170g, 160～170g, 160～170g, 160～170g, 1～3g and 0.5～5g with Chinese medicine oral liquid; Wherein antiseptic is sorbic acid, potassium sorbate or calcium sorbate; Correctives is Aspartane or stevioside.
The department of pediatrics of preparation control H1N1 virus is implemented according to the following steps with Chinese medicine oral liquid: one, elder generation decocts 0.5h with the Gypsum Fibrosum water of 350～450g, adding the Herba Ephedrae of 40～60g, the Semen Armeniacae Amarum of 90～110g, the Radix Glycyrrhizae of 40～60g, the Flos Lonicerae of 160～170g, the Fructus Forsythiae of 160～170g, the Rhizoma Anemarrhenae of 160～170g, the Radix Scutellariae of 160～170g, the Radix Isatidis of 160～170g, the Radix Ophiopogonis of 160～170g and the Herba Houttuyniae reuse decocting of 160～170g then boils 2 times, each 1h, then collecting decoction must mix decocting liquid; Two, will mix decocting liquid and filter, then be evaporated to relative density under 80 ℃ the condition and be 1.10～1.15 clear paste, naturally cool to room temperature, adding ethanol then, to contain the alcohol amount to the solution be 70%～80%, then stir, leave standstill 20～30h again, get mixed solution A; Three, mixed solution A is filtered, filtrate recycling ethanol and to be concentrated into relative density under 80 ℃ the condition be 1.20～1.25 clear paste, add water to 800～1200mL then, then stirring, is that 90～100h is handled in cold preservation under 4～7 ℃ the condition in temperature then, filters, filtrate adds the antiseptic of 1～3g and the correctives of 0.5～5g, add water to 800～1200mL then, stir, promptly get the department of pediatrics Chinese medicine oral liquid of preventing and treating H1N1 virus; Wherein antiseptic is sorbic acid, potassium sorbate or calcium sorbate in the step 3; Correctives is Aspartane or stevioside.
The department of pediatrics Chinese medicine oral liquid of control H1N1 virus of the present invention, have relieving asthma, antitussive, analgesic, eliminate the phlegm, the effect of antiallergic, anti-swine flu; Department of pediatrics of the present invention with Chinese medicine oral liquid as can be known through the experiment of pharmacological toxicology, the department of pediatrics of control H1N1 virus of the present invention is obvious with the Chinese medicine oral liquid effect, and safe without toxic side effect.
The specific embodiment
Technical solution of the present invention is not limited to the following cited specific embodiment, also comprises the combination in any between each specific embodiment.
The specific embodiment one: the department of pediatrics of present embodiment control H1N1 virus is used the Herba Ephedrae of Chinese medicine oral liquid by 40～60g, the Semen Armeniacae Amarum of 90～110g, the Gypsum Fibrosum of 350～450g, the Radix Glycyrrhizae of 40～60g, the Flos Lonicerae of 160～170g, the Fructus Forsythiae of 160～170g, the Rhizoma Anemarrhenae of 160～170g, the Radix Scutellariae of 160～170g, the Radix Isatidis of 160～170g, the Radix Ophiopogonis of 160～170g, the Herba Houttuyniae of 160～170g, the antiseptic of 1～3g and the correctives of 0.5～5g add the water of 800～1200mL and make; Wherein antiseptic is sorbic acid, potassium sorbate or calcium sorbate; Correctives is Aspartane or stevioside.
The specific embodiment two: the department of pediatrics of present embodiment control H1N1 virus with Chinese medicine oral liquid by the Radix Isatidis of the Radix Scutellariae of the Rhizoma Anemarrhenae of the Fructus Forsythiae of the Flos Lonicerae of the Radix Glycyrrhizae of the Gypsum Fibrosum of the Semen Armeniacae Amarum of the Herba Ephedrae of 50g, 100g, 400g, 50g, 167g, 167g, 167g, 167g, 167g, 167g Radix Ophiopogonis 167g Herba Houttuyniae, the water of the antiseptic of 2g and the correctives of 2g and 1000mL is made.
The present embodiment antiseptic is a potassium sorbate; Correctives is an Aspartane.
The department of pediatrics Chinese medicine oral liquid of the control H1N1 virus of present embodiment is a compound Chinese medicinal preparation, have the antitussive of relievining asthma, analgesic, eliminate the phlegm, effect such as antiallergic, anti-swine flu, the Chinese medicine oral liquid of present embodiment has been observed the main pharmacological of Chinese medicine oral liquid by animal experiment, tests as follows:
1, the department of pediatrics of the control H1N1 virus of present embodiment is carried out the antitussive test with Chinese medicine oral liquid to white mice
This test is adopted the ammonia inhalation that white mice is caused and is coughed.Oral administration 6 times (finishing in 4 days), 1h carries out the ammonia inhalation test after the last administration.EXPERIMENTAL DESIGN adopts " going up purgation ", and every group with 12～15 white mice, and observing in 1 minute white mice, 3 typical responders of coughing to occur positive, obtains respectively and respectively organize half antitussive time (EDT
50), causing that with matched group the mouse cough time is 100%, the administration group is that 130% above person of matched group is " effectively ", 150% above person is " produce effects ".The department of pediatrics of the control H1N1 virus of present embodiment after tested has very significantly antitussive effect with heavy dose of group of Chinese medicine oral liquid and middle dosage group, small dose group also has certain antitussive effect, and demonstrate certain dose-effect relationship, the half antitussive intensity of heavy dose of group (20mL/kg * 6 time) (ED relatively
50) be close with the antitussive intensity of positive controls codeine phosphate (100mg/kg * 1 time).
2, the department of pediatrics of the control H1N1 virus of present embodiment is separated heat test with Chinese medicine oral liquid to rat
The artificial pyrogenicity method of fresh yeast is adopted in this test.Be mixed with 15% yeast suspension with normal saline, it is subcutaneous to be injected in rat nape portion respectively, the 20.0mL/kg body weight.Measure twice of the anus temperature (two minor tick 1h) of every rat before the injection yeast, taking the mean is normal anus temperature.Respectively at 5h and each oral administration of 7h behind the injection yeast once, the anus temperature of 1h, 2h, 3h, 4h changes after the measurement first administration, represents with the anus temperature approach of different time after the gentle administration of normal anus.The department of pediatrics of the control H1N1 virus of present embodiment in 1～4h, has very significantly antipyretic effect with heavy dose of group of Chinese medicine oral liquid and middle dosage group (20.0mL/kg, 10.0mL/kg are 100.0g, 50.0g crude drug/kg body weight) after the administration.
3, the department of pediatrics of the control H1N1 virus of present embodiment is carried out expectorant test with Chinese medicine oral liquid to rat
The rat glass capillary method is adopted in this test.Oral administration is 3 times in two days, 1h after the last administration, and after the lumbar injection urethane 1g/kg anesthesia, operation separates trachea, hits exactly between two cartilaginous rings at the thyroid cartilage lower edge and pricks an aperture with No. 7 syringe needles, inserts capillary glass tube then and stablizes 1h; Postoperative 2h begins to collect the sputum of respiratory tract, and replace tubes at any time is in order to avoid liquid overflows, collect 5h altogether, with torsion balance weigh up collect behind the liquid and collect liquid before capillary tube weight, both differences are the weight of respiratory tract sputum, as the expectorant effect of estimating medicine.Merge 4 batches of experiment effects, and convert out the sputum secretory volume than being mg/100g the weight of animals/h.Positive drug TANKEJING (0.2g/kg) has the excretory effect of tangible promotion respiratory tract fluid by testing as can be known, but present embodiment obtains preventing and treating the department of pediatrics of H1N1 virus does not see tangible effect with the large and small dosage group of Chinese medicine oral liquid, and middle dosage group (5.0g/kg) has certain facilitation to the secretion of anesthesia rat respiratory tract sputum.
4, the department of pediatrics of the control H1N1 virus of present embodiment is drawn whole animal with Chinese medicine oral liquid and is breathed heavily method test
(1) Gastric lavage
Get surplus the Cavia porcellus 60 childhood body weight 150 ± 109, male and female dual-purpose.Divide six groups and label at random, 10 every group, several of remainders are stand-by in addition.Put into glass bell jar (4kg) one by one and spray into 2% acecoline and the mixed liquor 15s of 0.4% histamine phosphate (2: 1) with the pressure average rate of 400mmHg, observe drawing to breathe heavily incubation period and (promptly beginning to asthma attack of Cavia porcellus from spraying, the time that breathing is the devil and falls until tic), surpassing 120 seconds persons is insensitive person, will not select for use.
The department of pediatrics of getting the control H1N1 virus that each group of the Cavia porcellus of said determination after incubation period obtains with present embodiment respectively is with Chinese medicine oral liquid 5g/kg, 10g/kg, aminophylline sheet 20mg/kg (converting dose,equivalent ratio table by body surface area between humans and animals tries to achieve), XIAOKECHUAN 1ml/100g, normal saline 1ml/ only, three continuous gastric infusions in one day, again draw with said method behind the administration 1h for the third time and breathe heavily, measure it and draw and breathe heavily incubation period.(if the person of keeping one's legs calculates with 360s, promptly observing 6min after the administration).To comparing incubation period before and after each group administration, try to achieve the prolongation of latency time, and compare between organizing then.The department of pediatrics of the control H1N1 virus of present embodiment has antiasthmatic effect with Chinese medicine oral liquid more as can be known, relatively has the meaning of highly significant and its heavy dose of group of positive control drug XIAOKECHUAN group comparison also than XIAOKECHUAN the highly significant meaning to be arranged with the normal saline group.
(2) intraperitoneal injection method
Get 60 of Cavia porcelluss, be divided into 6 groups at random, 10 every group.The department of pediatrics of giving the control H1N1 virus that present embodiment obtains respectively is with Chinese medicine oral liquid 10g/kg, aminophylline injection 125mg/kg, XIAOKECHUAN 1ml/100g, a normal saline 1ml/ once abdominal cavity injection administration, 1h begins to survey incubation period after the administration, and surplus condition is with 4 (1).The department of pediatrics of the control H1N1 virus of present embodiment also is tangible antiasthmatic effect with the Chinese medicine oral liquid intraperitoneal injection, and the meaning of highly significant is all arranged than normal saline group and XIAOKECHUAN group.
5, the department of pediatrics of the control H1N1 virus of present embodiment is with the influence (vitro method) of Chinese medicine oral liquid to Guinea pig lung bronchus perfusion flow
The bronchus that is prepared in advance perfusion device is got healthy guinea pig 250g, and 6, hit unconsciously, cut the carotid artery blood-letting, and open the thoracic cavity exposure heart and lungs rapidly, cut one section trachea, take out outside the thoracic cavity together with heart and lungs.At once 37 ℃ of lung immersions are contained in the culture dish of oxygen krebs solution, push lungs gently for several times, to discharge the gas in the lung.The reuse syringe is got krebs solution number milliliter, in trachea injects lung, makes atelectasis.Then trachea is tied in line on the sleeve pipe of perfusion device, open square folder, with the krebs solution perfusion it.On the lung surface with tens of being dispersed in property of syringe needle perforation, regulating perfusion rate is 2～3ml for the per minute liquid outflow amount, after treating that perfusion flow is constant, by injecting normal saline, 0.5% aminophylline, 1.5% each 0.5mL of infantile lung heat cough with asthma oral liquid in the rubber tube of cannula tip respectively, 30s after the medication, the every min liquid outflow amount of opening entry.The department of pediatrics of the control H1N1 virus of present embodiment can increase Guinea pig lung bronchus perfusion flow with Chinese medicine oral liquid.
6, the department of pediatrics of the control H1N1 virus of present embodiment is with the effect of Chinese medicine oral liquid to the antagonism anaphylactic shock of rat
Get 60 of healthy white rats, male and female half and half, body weight 90～100g.Every Mus lumbar injection 20% fresh Ovum Gallus domesticus album normal saline solution 2mL and bacillus pertussis vaccine 0.2mL sensitization, random packet then, 10 every group.Began the same day respectively with infantile lung heat cough with asthma oral liquid 5g/kg/ days, 10g/kg/ days, 20g/kg/ days; XIAOKECHUAN 1mL//day, hismanal 0.18mg/kg/ days, normal saline 1mL//day gastric infusion, successive administration after 14 days once more with 20% fresh Ovum Gallus domesticus album, a normal saline 2mL/ lumbar injection carries out secondary antigen and attacks, and makes rat that anaphylactic shock death take place.Compare medication group and control animals mortality rate.The P value is asked in X 2 test.The Chinese medicine oral liquid antianaphylaxis shock effect of the department of pediatrics of the control H1N1 virus of present embodiment; the relatively more equal significance of animal dead number and negative control group and positive controls shows that the department of pediatrics of the control H1N1 virus of present embodiment has the certain protection effect with Chinese medicine oral liquid to the rat anaphylactic shock as a result.
The department of pediatrics of the control H1N1 virus of the present embodiment main toxicological effect of Chinese medicine oral liquid, test as follows:
The department of pediatrics of the control H1N1 virus of present embodiment is carried out long-term toxicity test for animals with Chinese medicine oral liquid
The Wistar rat is used in this test, and oral administration (filling stomach) gives the Chinese medicine oral liquid 6gkg of present embodiment
-1Body weight (is amounted to crude drug amount 40.8gkg
-1Body weight), be equivalent to clinical plan dosage (crude drug amount 0.757gkg
-1Body weight) 53.8 times.Continuously 12 weeks of gastric infusion, reversibility observed for 2 weeks.Experimental result shows, reversibility is observed after administration phase and drug withdrawal, and the animal subject general state is good, four groups of rat appetite and body weight gain basically identical during the administration; Routine blood test and blood biochemical detect and show that all said preparation all has no adverse effects to animal subject hemopoietic system, liver function, renal function; Pathological examination is not seen the pathological change relevant with toxicity under naked eyes and the mirror; No Secondary cases toxic reaction takes place after the drug withdrawal.
By above-mentioned toxicological experiment as can be known, the department of pediatrics of the control H1N1 virus of present embodiment is with the Chinese medicine oral liquid safety non-toxic and have no side effect.
The department of pediatrics of present embodiment control H1N1 virus has anti-swine flu effect with Chinese medicine oral liquid:
The department of pediatrics of present embodiment control H1N1 virus with Chinese medicine oral liquid 10～November of this year in Beijing 3 tame front three hospital application make a definite diagnosis swine flu or suspected case in 101 examples, wherein 91 examples are generated heat.
The clinical practice result shows: the average fever time of 91 routine fever patients 1.82 days; The cough symptom significantly weakened natural law 2.1 days; The transference cure natural law of having sore throat is 2.6 days.
The specific embodiment three: the department of pediatrics of present embodiment preparation control H1N1 virus is implemented according to the following steps with Chinese medicine oral liquid: one, elder generation decocts 0.5h with the Gypsum Fibrosum water of 350～450g, adding the Herba Ephedrae of 40～60g, the Semen Armeniacae Amarum of 90～110g, the Radix Glycyrrhizae of 40～60g, the Flos Lonicerae of 160～170g, the Fructus Forsythiae of 160～170g, the Rhizoma Anemarrhenae of 160～170g, the Radix Scutellariae of 160～170g, the Radix Isatidis of 160～170g, the Radix Ophiopogonis of 160～170g and the Herba Houttuyniae reuse decocting of 160～170g then boils 2 times, each 1h, then collecting decoction must mix decocting liquid; Two, will mix decocting liquid and filter, then be evaporated to relative density under 80 ℃ the condition and be 1.10～1.15 clear paste, naturally cool to room temperature, adding ethanol then, to contain the alcohol amount to the solution be 70%～80%, then stir, leave standstill 20～30h again, get mixed solution A; Three, mixed solution A is filtered, filtrate recycling ethanol and to be concentrated into relative density under 80 ℃ the condition be 1.20～1.25 clear paste, add water to 800～1200mL then, then stirring, is that 90～100h is handled in cold preservation under 4～7 ℃ the condition in temperature then, filters, filtrate adds the antiseptic of 1～3g and the correctives of 0.5～5g, add water to 800～1200mL then, stir, promptly get the department of pediatrics Chinese medicine oral liquid of preventing and treating H1N1 virus; Wherein antiseptic is sorbic acid, potassium sorbate or calcium sorbate in the step 3; Correctives is Aspartane or stevioside.
The specific embodiment four: what present embodiment and the specific embodiment three were different is that step 1 decocts 0.5h with the Gypsum Fibrosum water of 400g earlier, adds the Herba Ephedrae of 50g, the Semen Armeniacae Amarum of 100g, the Radix Glycyrrhizae of 50g, the Flos Lonicerae of 167g, the Fructus Forsythiae of 167g, the Rhizoma Anemarrhenae of 167g, the Radix Scutellariae of 167g, the Radix Isatidis of 167g, the Radix Ophiopogonis of 167g and the Herba Houttuyniae reuse decocting of 167g then and boils 2 times.Other step and parameter are identical with the specific embodiment three.
The specific embodiment five: present embodiment is different with the specific embodiment three or four is that to be evaporated to relative density under 80 ℃ the condition in the step 2 be 1.13 clear paste.Other step and parameter are identical with the specific embodiment three or four.。
The specific embodiment six: present embodiment and the specific embodiment three to five are different is that to contain the alcohol amount in the step 2 be 75%.Other step and parameter are identical with the specific embodiment three to five.
The specific embodiment seven: what present embodiment and the specific embodiment three to six were different is that time of repose is 24h in the step 2.Other step and parameter are identical with the specific embodiment three to six.
The specific embodiment eight: present embodiment and the specific embodiment three to seven are different is filtrate recycling ethanol and to be concentrated into relative density under 80 ℃ the condition be 1.22 clear paste in the step 3.Other step and parameter are identical with the specific embodiment three to seven.
The specific embodiment nine: present embodiment and the specific embodiment three to eight are different be in the step 3 cold preservation processing time be 96h.Other step and parameter are identical with the specific embodiment three to eight.
The specific embodiment ten: the department of pediatrics of present embodiment preparation control H1N1 virus is implemented according to the following steps with Chinese medicine oral liquid: one, elder generation decocts 0.5h with the Gypsum Fibrosum water of 400g, adding the Herba Ephedrae of 50g, the Semen Armeniacae Amarum of 100g, the Radix Glycyrrhizae of 50g, the Flos Lonicerae of 167g, the Fructus Forsythiae of 167g, the Rhizoma Anemarrhenae of 167g, the Radix Scutellariae of 167g, the Radix Isatidis of 167g, the Radix Ophiopogonis of 167g and the Herba Houttuyniae reuse decocting of 167g then boils 2 times, each 1h, then collecting decoction must mix decocting liquid; Two, will mix decocting liquid and filter, and then be evaporated to relative density under 80 ℃ the condition and be 1.12 clear paste, and naturally cool to room temperature, adding ethanol then, to contain the alcohol amount to the solution be 75%, then stirs, and leaves standstill 24h again, mixed solution A; Three, mixed solution A is filtered, filtrate recycling ethanol and to be concentrated into relative density under 80 ℃ the condition be 1.24 clear paste, adding water to 1000mL then, then stir, is that 96h is handled in cold preservation under 6 ℃ the condition in temperature then, filter, filtrate adds the antiseptic of 2g and the correctives of 2g, adds water to 1000mL then, stirs, promptly get the department of pediatrics Chinese medicine oral liquid of preventing and treating H1N1 virus, promptly get the department of pediatrics Chinese medicine oral liquid of preventing and treating H1N1 virus.
Antiseptic is a sorbic acid in the present embodiment step 3; Correctives is an Aspartane.
The department of pediatrics Chinese medicine oral liquid of the control H1N1 virus that present embodiment obtains is a compound Chinese medicinal preparation, have relieving asthma, antitussive, analgesic, eliminate the phlegm, effect such as antiallergic, anti-swine flu, the Chinese medicine oral liquid of present embodiment has been observed the main pharmacological of Chinese medicine oral liquid by animal experiment, tests as follows:
1, the department of pediatrics of the control H1N1 virus that obtains of present embodiment is carried out the antitussive test with Chinese medicine oral liquid to white mice
This test is adopted the ammonia inhalation that white mice is caused and is coughed.Oral administration 6 times (finishing in 4 days), 1h carries out the ammonia inhalation test after the last administration.EXPERIMENTAL DESIGN adopts " going up purgation ", and every group with 12～15 white mice, and observing in 1 minute white mice, 3 typical responders of coughing to occur positive, obtains respectively and respectively organize half antitussive time (EDT
50), causing that with matched group the mouse cough time is 100%, the administration group is that 130% above person of matched group is " effectively ", 150% above person is " produce effects ".The results are shown in Table 1.
Table 1
Result by table 1 shows, the department of pediatrics of the control H1N1 virus that present embodiment obtains has very significantly antitussive effect with heavy dose of group of Chinese medicine oral liquid and middle dosage group, small dose group also has certain antitussive effect, and demonstrate certain dose-effect relationship, the half antitussive intensity of heavy dose of group (20mL/kg * 6 time) (ED relatively
50) be close with the antitussive intensity of positive controls codeine phosphate (100mg/kg * 1 time).
2, the department of pediatrics of the control H1N1 virus that obtains of present embodiment is separated heat test with Chinese medicine oral liquid to rat
The artificial pyrogenicity method of fresh yeast is adopted in this test.Be mixed with 15% yeast suspension with normal saline, it is subcutaneous to be injected in rat nape portion respectively, the 20.0mL/kg body weight.Measure twice of the anus temperature (two minor tick 1h) of every rat before the injection yeast, taking the mean is normal anus temperature.Respectively at 5h and each oral administration of 7h behind the injection yeast once, the anus temperature of 1h, 2h, 3h, 4h changes after the measurement first administration, represents with the anus temperature approach of different time after the gentle administration of normal anus.The results are shown in Table 2.
Table 2
Annotate: with relatively t check of matched group, △ shows that P is near 0.05; * show P＜0.05; * shows P＜0.01; * * shows P＜0.001; Following table all with.
Result by table 2 shows, dosage group (20.0mL/kg, 10.0mL/kg are 100.0g, 50.0g crude drug/kg body weight) during the department of pediatrics of the control H1N1 virus that present embodiment obtains reaches with the heavy dose of group of Chinese medicine oral liquid, in 1～4h, very significantly antipyretic effect is arranged after the administration.
3, the department of pediatrics of the control H1N1 virus that obtains of present embodiment is carried out expectorant test with Chinese medicine oral liquid to rat
The rat glass capillary method is adopted in this test.Oral administration is 3 times in two days, 1h after the last administration, and after the lumbar injection urethane 1g/kg anesthesia, operation separates trachea, hits exactly between two cartilaginous rings at the thyroid cartilage lower edge and pricks an aperture with No. 7 syringe needles, inserts capillary glass tube then and stablizes 1h; Postoperative 2h begins to collect the sputum of respiratory tract, and replace tubes at any time is in order to avoid liquid overflows, collect 5h altogether, with torsion balance weigh up collect behind the liquid and collect liquid before capillary tube weight, both differences are the weight of respiratory tract sputum, as the expectorant effect of estimating medicine.Merge 4 batches of experiment effects, and convert out the sputum secretory volume than being mg/100g the weight of animals/h.The results are shown in Table 3.
Table 3
Annotate: TANKEJING forms by multiple medicines such as Radix Platycodonis 15.6%, Semen Armeniacae Amarum 1.25%, Borneolum Syntheticum 16%, Radix Glycyrrhizae 12.4% are refining.
The result shows by table 3, positive drug TANKEJING (0.2g/kg) has the excretory effect of tangible promotion respiratory tract fluid, but present embodiment obtains preventing and treating the department of pediatrics of H1N1 virus does not see tangible effect with the large and small dosage group of Chinese medicine oral liquid, and middle dosage group (5.0g/kg) has certain facilitation (P＜0.05) to the secretion of anesthesia rat respiratory tract sputum.
4, the department of pediatrics of the control H1N1 virus that obtains of present embodiment is drawn whole animal with Chinese medicine oral liquid and is breathed heavily the method test
(1) Gastric lavage
Get surplus the Cavia porcellus 60 childhood body weight 150 ± 109, male and female dual-purpose.Divide six groups and label at random, 10 every group, several of remainders are stand-by in addition.Put into glass bell jar (4kg) one by one and spray into 2% acecoline and the mixed liquor 15s of 0.4% histamine phosphate (2: 1) with the pressure average rate of 400mmHg, observe drawing to breathe heavily incubation period and (promptly beginning to asthma attack of Cavia porcellus from spraying, the time that breathing is the devil and falls until tic), surpassing 120 seconds persons is insensitive person, will not select for use.
The department of pediatrics of getting the control H1N1 virus that each group of the Cavia porcellus of said determination after incubation period obtains with present embodiment respectively is with Chinese medicine oral liquid 5g/kg, 10g/kg, aminophylline sheet 20mg/kg (converting dose,equivalent ratio table by body surface area between humans and animals tries to achieve), XIAOKECHUAN 1ml/100g, normal saline 1ml/ only, three continuous gastric infusions in one day, again draw with said method behind the administration 1h for the third time and breathe heavily, measure it and draw and breathe heavily incubation period.(if the person of keeping one's legs calculates with 360s, promptly observing 6min after the administration).To comparing incubation period before and after each group administration, try to achieve the prolongation of latency time, and compare between organizing then.The results are shown in Table 4.
Table 4
Annotate: P
1For comparing P with the normal saline group
2For comparing P with the XIAOKECHUAN group
3For comparing with the aminophylline group.
The department of pediatrics that shows the control H1N1 virus that present embodiment obtains by table 4 result has antiasthmatic effect with Chinese medicine oral liquid, relatively has its heavy dose of group of meaning (P＜0.01) and the comparison of positive control drug XIAOKECHUAN group of highly significant also than XIAOKECHUAN highly significant meaning (P＜0.01) to be arranged with the normal saline group.
(2) intraperitoneal injection method
Get 60 of Cavia porcelluss, be divided into 6 groups at random, 10 every group.The department of pediatrics of giving the control H1N1 virus that present embodiment obtains respectively is with Chinese medicine oral liquid 10g/kg, aminophylline injection 125mg/kg, XIAOKECHUAN 1ml/100g, a normal saline 1ml/ once abdominal cavity injection administration, 1h begins to survey incubation period after the administration, and surplus condition is with 4 (1).The results are shown in Table 5.
Table 5
Annotate: P
1For comparing with the normal saline group; P
2For comparing with the XIAOKECHUAN group; P
3For comparing with the aminophylline group
The department of pediatrics that shows the control H1N1 virus that present embodiment obtains by table 5 result also is tangible antiasthmatic effect with the Chinese medicine oral liquid intraperitoneal injection, and the meaning (P＜0.01, P＜0.01) of highly significant is all arranged than normal saline group and XIAOKECHUAN group.
5, the department of pediatrics of the control H1N1 virus that obtains of present embodiment is with the influence (vitro method) of Chinese medicine oral liquid to Guinea pig lung bronchus perfusion flow
The bronchus that is prepared in advance perfusion device is got healthy guinea pig 250g, and 6, hit unconsciously, cut the carotid artery blood-letting, and open the thoracic cavity exposure heart and lungs rapidly, cut one section trachea, take out outside the thoracic cavity together with heart and lungs.At once 37 ℃ of lung immersions are contained in the culture dish of oxygen krebs solution, push lungs gently for several times, to discharge the gas in the lung.The reuse syringe is got krebs solution number milliliter, in trachea injects lung, makes atelectasis.Then trachea is tied in line on the sleeve pipe of perfusion device, open square folder, with the krebs solution perfusion it.On the lung surface with tens of being dispersed in property of syringe needle perforation, regulating perfusion rate is 2～3ml for the per minute liquid outflow amount, after treating that perfusion flow is constant, by injecting normal saline, 0.5% aminophylline, 1.5% each 0.5mL of infantile lung heat cough with asthma oral liquid in the rubber tube of cannula tip respectively, 30s after the medication, the every min liquid outflow amount of opening entry.The results are shown in Table 6.
Table 6
P in the table 6
1For with administration after and before the self administration relatively; P
2For with the administration of normal saline group after compare.
The department of pediatrics of the control H1N1 virus that is obtained by the visible present embodiment of table 6 can increase Guinea pig lung bronchus perfusion flow with Chinese medicine oral liquid.
6, the department of pediatrics of the control H1N1 virus that obtains of present embodiment is with the effect of Chinese medicine oral liquid to the antagonism anaphylactic shock of rat
Get 60 of healthy white rats, male and female half and half, body weight 90～100g.Every Mus lumbar injection 20% fresh Ovum Gallus domesticus album normal saline solution 2mL and bacillus pertussis vaccine 0.2mL sensitization, random packet then, 10 every group.Began the same day respectively with infantile lung heat cough with asthma oral liquid 5g/kg/ days, 10g/kg/ days, 20g/kg/ days; XIAOKECHUAN 1mL//day, hismanal 0.18mg/kg/ days, normal saline 1mL//day gastric infusion, successive administration after 14 days once more with 20% fresh Ovum Gallus domesticus album, a normal saline 2mL/ lumbar injection carries out secondary antigen and attacks, and makes rat that anaphylactic shock death take place.Compare medication group and control animals mortality rate.The P value is asked in X 2 test.The results are shown in Table 7.
Table 7
P in the table 7
1For comparing with the XIAOKECHUAN group; P
2For comparing with the hismanal group; P
3For comparing with the normal saline group.
The department of pediatrics of the control H1N1 virus that is obtained by the visible present embodiment of table 7 is with the effect of Chinese medicine oral liquid antianaphylaxis shock; relatively equal significance (P＜0.05 of animal dead number and negative control group and positive controls as a result; P＜0.05), the department of pediatrics that shows the control H1N1 virus that present embodiment obtains has the certain protection effect with Chinese medicine oral liquid to the rat anaphylactic shock.
The department of pediatrics of the control H1N1 virus that present embodiment the obtains main toxicological effect of Chinese medicine oral liquid, test as follows:
The department of pediatrics of the control H1N1 virus that present embodiment obtains is carried out long-term toxicity test for animals with Chinese medicine oral liquid
The Wistar rat is used in this test, and oral administration (filling stomach) gives the Chinese medicine oral liquid 6gkg of present embodiment
-1Body weight (is amounted to crude drug amount 40.8gkg
-1Body weight), be equivalent to clinical plan dosage (crude drug amount 0.757gkg
-1Body weight) 53.8 times.Continuously 12 weeks of gastric infusion, reversibility observed for 2 weeks.Experimental result shows, reversibility is observed after administration phase and drug withdrawal, and the animal subject general state is good, four groups of rat appetite and body weight gain basically identical during the administration; Routine blood test and blood biochemical detect and show that all said preparation all has no adverse effects to animal subject hemopoietic system, liver function, renal function; Pathological examination is not seen the pathological change relevant with toxicity under naked eyes and the mirror; No Secondary cases toxic reaction takes place after the drug withdrawal.
By above-mentioned toxicological experiment as can be known, the department of pediatrics of the control H1N1 virus that obtains of present embodiment is with the Chinese medicine oral liquid safety non-toxic and have no side effect.
The department of pediatrics of the control H1N1 virus that present embodiment obtains has anti-swine flu effect with Chinese medicine oral liquid:
The department of pediatrics of present embodiment control H1N1 virus with Chinese medicine oral liquid 10～November of this year in Beijing 3 tame front three hospital application make a definite diagnosis swine flu or suspected case in 101 examples, wherein 91 examples are generated heat.
The clinical practice result shows: the average fever time of 91 routine fever patients 1.82 days; The cough symptom significantly weakened natural law 2.1 days; The transference cure natural law of having sore throat is 2.6 days.
Claims (8)
1. department of pediatrics Chinese medicine oral liquid of preventing and treating H1N1 virus, the department of pediatrics that it is characterized in that preventing and treating H1N1 virus is made by the water that the correctives of the antiseptic of the Herba Houttuyniae of Radix Ophiopogonis of the Radix Isatidis of the Radix Scutellariae of the Rhizoma Anemarrhenae of the Fructus Forsythiae of the Flos Lonicerae of the Radix Glycyrrhizae of the Gypsum Fibrosum of the Semen Armeniacae Amarum of the Herba Ephedrae of 40～60g, 90～110g, 350～450g, 40～60g, 160～170g, 160～170g, 160～170g, 160～170g, 160～170g, 160～170g, 160～170g, 1～3g and 0.5～5g adds 800～1200mL with Chinese medicine oral liquid; Wherein antiseptic is sorbic acid, potassium sorbate or calcium sorbate; Correctives is Aspartane or stevioside.
2. a kind of department of pediatrics Chinese medicine oral liquid of preventing and treating H1N1 virus according to claim 1, the department of pediatrics that it is characterized in that preventing and treating H1N1 virus with Chinese medicine oral liquid by the Radix Isatidis of the Radix Scutellariae of the Rhizoma Anemarrhenae of the Fructus Forsythiae of the Flos Lonicerae of the Radix Glycyrrhizae of the Gypsum Fibrosum of the Semen Armeniacae Amarum of the Herba Ephedrae of 50g, 100g, 400g, 50g, 167g, 167g, 167g, 167g, 167g, 167g Radix Ophiopogonis 167g the antiseptic of Herba Houttuyniae, 2g and the correctives of the 2g water that adds 1000mL make.
3. prevent and treat the preparation method of the department of pediatrics of H1N1 virus as one kind of claim 1 with Chinese medicine oral liquid, it is characterized in that preparing the department of pediatrics of preventing and treating H1N1 virus implements according to the following steps with Chinese medicine oral liquid: one, Gypsum Fibrosum water with 350～450g decocts 0.5h earlier, the Herba Ephedrae that adds 40～60g then, the Semen Armeniacae Amarum of 90～110g, the Radix Glycyrrhizae of 40～60g, the Flos Lonicerae of 160～170g, the Fructus Forsythiae of 160～170g, the Rhizoma Anemarrhenae of 160～170g, the Radix Scutellariae of 160～170g, the Radix Isatidis of 160～170g, the Herba Houttuyniae reuse decocting of the Radix Ophiopogonis of 160～170g and 160～170g boils 2 times, each 1h, then collecting decoction must mix decocting liquid; Two, will mix decocting liquid and filter, then be evaporated to relative density under 80 ℃ the condition and be 1.10～1.15 clear paste, naturally cool to room temperature, adding ethanol then, to contain the alcohol amount to the solution be 70%～80%, then stir, leave standstill 20～30h again, get mixed solution A; Three, mixed solution A is filtered, filtrate recycling ethanol and to be concentrated into relative density under 80 ℃ the condition be 1.20～1.25 clear paste, add water to 800～1200mL then, then stirring, is that 90～100h is handled in cold preservation under 4～7 ℃ the condition in temperature then, filters, filtrate adds the antiseptic of 1～3g and the correctives of 0.5～5g, add water to 800～1200mL then, stir, promptly get the department of pediatrics Chinese medicine oral liquid of preventing and treating H1N1 virus; Wherein antiseptic is sorbic acid, potassium sorbate or calcium sorbate in the step 3; Correctives is Aspartane or stevioside.
4. a kind of department of pediatrics of preventing and treating H1N1 virus according to claim 3 is with the preparation method of Chinese medicine oral liquid, it is characterized in that being evaporated in the step 2 relative density under 80 ℃ the condition and be 1.13 clear paste.
5. according to claim 3 or 4 described a kind of preparation methoies of preventing and treating the department of pediatrics of H1N1 virus with Chinese medicine oral liquid, it is characterized in that containing in the step 2 alcohol amount is 75%.
6. a kind of preparation method of preventing and treating the department of pediatrics of H1N1 virus with Chinese medicine oral liquid according to claim 5 is characterized in that time of repose is 24h in the step 2.
7. according to claim 3,4 or 6 described a kind of department of pediatrics of the preventing and treating H1N1 virus preparation methoies with Chinese medicine oral liquid, it is characterized in that filtrate recycling ethanol in the step 3 and being concentrated into relative density under 80 ℃ the condition is 1.22 clear paste.
8. a kind of preparation method of preventing and treating the department of pediatrics of H1N1 virus with Chinese medicine oral liquid according to claim 7 is characterized in that the cold preservation processing time is 96h in the step 3.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106309769A (en) * | 2016-07-27 | 2017-01-11 | 陈华平 | Traditional Chinese medicine composition for treating influenza type A |
| CN110200907A (en) * | 2019-06-21 | 2019-09-06 | 葵花药业集团北京药物研究院有限公司 | A kind of infantile lung heat cough and asthma suspension drops of fruity and preparation method thereof |
| CN111358892A (en) * | 2020-04-23 | 2020-07-03 | 徐修信 | Traditional Chinese medicine composition for treating novel coronavirus pneumonia in early and middle stages and preparation method thereof |
-
2009
- 2009-12-21 CN CN200910073468A patent/CN101711831A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106309769A (en) * | 2016-07-27 | 2017-01-11 | 陈华平 | Traditional Chinese medicine composition for treating influenza type A |
| CN110200907A (en) * | 2019-06-21 | 2019-09-06 | 葵花药业集团北京药物研究院有限公司 | A kind of infantile lung heat cough and asthma suspension drops of fruity and preparation method thereof |
| CN111358892A (en) * | 2020-04-23 | 2020-07-03 | 徐修信 | Traditional Chinese medicine composition for treating novel coronavirus pneumonia in early and middle stages and preparation method thereof |
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Open date: 20100526 |