CN101672815B - Standard solution used for electrolytic analyzer linear calibration - Google Patents
Standard solution used for electrolytic analyzer linear calibration Download PDFInfo
- Publication number
- CN101672815B CN101672815B CN 200910196844 CN200910196844A CN101672815B CN 101672815 B CN101672815 B CN 101672815B CN 200910196844 CN200910196844 CN 200910196844 CN 200910196844 A CN200910196844 A CN 200910196844A CN 101672815 B CN101672815 B CN 101672815B
- Authority
- CN
- China
- Prior art keywords
- standard solution
- solution
- sodium
- potassium
- chlorine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The invention provides a standard solution used for electrolytic analyzer linear calibration, which is composed of five groups of different-concentration solutions containing potassium ion, sodion and chloridion. The concentration range of the three ions in the standard solution is respectively as follows: 2-8mmol/L of K, 100-180mmol/L of Na, 60-140mmol/L of Cl, and the pH range of the solutions is 7.3-7.5. The invention also discloses the preparing method of the standard solution; the invention provides a widely-used standard solution used for electrolytic analyzer linear calibration and provides guarantee for accurately evaluating instrument performance index.
Description
Technical field
The present invention relates to clinical examination calibrating and the transmission of quantity value field of metrology and measurement, particularly a kind of standard solution used for electrolytic analyzer linear calibration of blomelicalbloodgasandelectrolrteanalyzers.
Background technology
In medical diagnosis on disease, the doctor often will measure the electrolyte concentration of patient's body fluid such as blood, and the data obtained is as the basis for estimation to disease.Wherein, flame photometry is that the Sodium in Serum potassium that International Federation of Clinical Chemistry (IFCC) is recommended detects reference method.But, the complicated operation of flame photometer, and contaminated air, analysis time is long, and can only measure each time the concentration of a certain ion among the human electrolyte.Therefore the method is progressively replaced by blomelicalbloodgasandelectrolrteanalyzers institute.
At present, blomelicalbloodgasandelectrolrteanalyzers is mainly used in the electrolyte ingredient analysis in clinical treatment human body serum and the urine, take potassium, sodium, chlorion as main detected object.As an important step in the clinical examination quality control, standard committee of U.S. clinical labororatory (NCCLS) has issued EP～6P normative document " quantitative detecting method linear evaluation ", and the linear evaluation of quantitative detection is claimed.Linear index is the important content of clinical laboratory information system quality control, also is accurately and reliably important guarantee of blomelicalbloodgasandelectrolrteanalyzers testing result.
The linear calibration that blomelicalbloodgasandelectrolrteanalyzers is used is with standard solution and be different from the linear calibration standard solution of common meaning.Because the principle of the blomelicalbloodgasandelectrolrteanalyzers that uses in the Clinical Test Lab at present is based on ion-selective electrode to the specificly-response of specific ion, yet the electrode performance that the instrument of each producer's different model uses is all variant, namely different to the influential ion of its selectivity.If in calibrating solution, introduced the ion that ion-selective electrode is had response, will bring impact to the accuracy of evaluation result.
Therefore, need to be a kind of specially for standard solution used for electrolytic analyzer linear calibration, for accurate evaluation appts performance index are given security.
The linear solution that has at present blomelicalbloodgasandelectrolrteanalyzers when calibrating of bibliographical information to use is to analyze pure or other chemical reagent of higher level is directly prepared mostly.Such solution in use has certain limitation, the main problem that has following two aspects:
1. solution concentration does not have accurately definite value, lacks the foundation of magnitude tracing.Conventional chemical reagent is the minimum content of mark sample in explanation only, and this solution that just causes preparing can not provide accurate value, can't carry out transmission of quantity value and trace to the source;
2. coexisting ion disturbs analytical approach.Because each detection system causes measuring noisy ion also different for different ions difference optionally.Therefore the coexisting ion beyond the detected object of introducing in process for preparation will inevitably affect the general applicability of solution.For example, the acetate ion (Ac ~) of coexistence is introduced in the at present daily demarcation of part autogamy instrument in process for preparation with reagent.Show according to test, the chlorion working electrode of part model instrument is to Ac ~ have response, and increases with Ac ~ Enrichment chlorine ion concentration indicating value, causes test result to be false positive.
3. do not possess broad applicability.
In sum, this area lacks a kind of standard solution used for electrolytic analyzer linear calibration with broad applicability, for accurate evaluation appts performance index are given security.
Therefore, this area is in the urgent need to a kind of linear calibration standard solution that the accurate evaluation result is provided and has broad applicability for blomelicalbloodgasandelectrolrteanalyzers.
Summary of the invention
The first purpose of the present invention is to obtain a kind of standard solution used for electrolytic analyzer linear calibration with broad applicability, for accurate evaluation appts performance index are given security.
The second purpose of the present invention is to obtain a kind of compound method with standard solution used for electrolytic analyzer linear calibration of broad applicability, for accurate evaluation appts performance index are given security.
In a first aspect of the present invention, a kind of standard solution used for electrolytic analyzer linear calibration is provided, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of at least 5 group variable concentrations, the described concentration range that contains the solution of potassium, sodium, three kinds of ions of chlorine is respectively: potassium (K) 2mmol/L～8mmol/L, sodium (Na) 100mmol/L～180mmol/L, chlorine (Cl) 60mmol/L～140mmol/L;
The pH scope of described standard solution is between 7.3～7.5.
In a specific embodiment of the present invention, the pH scope of described standard solution is regulated by 3-morpholine propane sulfonic acid (MOPS) buffering agent.
In a specific embodiment of the present invention, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5～8 groups of variable concentrations.
In a specific embodiment of the present invention, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5 groups of variable concentrations.
In a specific embodiment of the present invention, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5 groups of variable concentrations, and the concentration of these 5 groups of solution with the mmol/L score is not:
Potassium ion: X
1, X
2, X
3, X
4, X
5
Sodion: Y
1, Y
2, Y
3, Y
4, Y
5
Chlorion: Z
1, Z
2, Z
3, Z
4, Z
5
Wherein,
X
1＝2～4、X
2＝3～5、X
3＝4～6、X
4＝5～7、X
5＝6～8；
Y
1＝100～120、Y
2＝110～130、Y
3＝130～150、Y
4＝150～170、Y
5＝160～180；
Z
1＝60～80、Z
2＝70～90、Z
3＝90～110、Z
4＝110～130、Z
5＝120～140；
And meet the following conditions: X
1＜X
2＜X
3＜X
4＜X
5Y
1＜Y
2＜Y
3＜Y
4＜Y
5Z
1＜Z
2＜Z
3＜Z
4＜Z
5
In a specific embodiment of the present invention, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5 groups of variable concentrations, and the concentration of these 5 groups of solution with the mmol/L score is not:
Potassium ion: 2,4,5,6,8;
Sodion: 100,130,140,150,180;
Chlorion: 60,90,100,110,140.
In a specific embodiment of the present invention, the described solution that contains potassium, sodium, three kinds of ions of chlorine is formulated by desired concn by sodium chloride, potassium chloride, sodium carbonate standard substance.
In a specific embodiment of the present invention, the solvent of described standard solution is electronic grade ultrapure water.
A second aspect of the present invention provides a kind of compound method of standard solution used for electrolytic analyzer linear calibration of the present invention, it is take sodium chloride, potassium chloride, sodium carbonate standard substance as the reagent main body, add buffering agent and regulate required pH value, formulated according to desired concn.
In a specific embodiment of the present invention, described buffering agent is 3-morpholine propane sulfonic acid (MOPS).
Embodiment
The inventor, has obtained for standard solution used for electrolytic analyzer linear calibration by improving preparation technology through extensive and deep research, for accurate evaluation appts performance index are given security.Finished on this basis the present invention.
Unless otherwise defined or explanation, the meaning that all specialties used herein are familiar with scientific words and those skilled in the art is identical.Any method similar or impartial to described content and material all can be applicable in the inventive method in addition.All reagent are commercially available getting.Below describe in detail to various aspects of the present invention:
Standard solution used for electrolytic analyzer linear calibration
Serial solution of the present invention is at least 5 groups, and wherein potassium, sodium, chlorine ion concentration are respectively: potassium (K) 2mmol/L～8mmol/L, sodium (Na) 100mmol/L～180mmol/L, chlorine (Cl) 60mmol/L～140mmol/L.
Particularly, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5～8 groups of variable concentrations.If the group number very little, then the effect of linear calibration is relatively poor even can't reach requirement; If the group number increases, also can reach goal of the invention of the present invention, but cost strengthens.
More preferably, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5 groups of variable concentrations.More specifically, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5 groups of variable concentrations, and its concentration is as shown in the table respectively: (unit is mmol/l)
| First group | Second group | The 3rd group | The 4th group | The 5th group | |
| Potassium | X 1 | X 2 | X 3 | X 4 | X 5 |
| Sodium | Y 1 | Y 2 | Y 3 | Y 4 | Y 5 |
| Chlorine | Z 1 | Z 2 | Z 3 | Z 4 | Z 5 |
Wherein:
The span of 5 groups of potassium, sodium, three kinds of ions of chlorine is as shown in the table: (unit is mmol/l)
| First group | Second group | The 3rd group | The 4th group | The 5th group | |
| Potassium | 2～4 | 3～5 | 4～6 | 5～7 | 6～8 |
| Sodium | 100～120 | 110～130 | 130～150 | 150～170 | 160～180 |
| Chlorine | 60～80 | 70～90 | 90～110 | 110～130 | 120～140 |
The span of 5 groups of potassium, sodium, three kinds of ions of chlorine and meeting the following conditions: X
1＜X
2＜X
3＜X
4＜X
5Y
1＜Y
2＜Y
3＜Y
4＜Y
5Z
1＜Z
2＜Z
3＜Z
4＜Z
5
More preferably, meet the following conditions:
The interval of the concentration of potassium (also is X
n～X
N-1, be between 1～2 n=5);
The interval of the concentration of sodium (also is Y
n～Y
N-1, be between 10～30 n=5);
The interval of the concentration of chlorine (also is Z
n～Z
N-1, be between 10～30 n=5);
More specifically, the span of 5 groups of potassium, sodium, three kinds of ions of chlorine is as shown in the table:
The described solution that contains potassium, sodium, three kinds of ions of chlorine is preferably formulated by sodium chloride, potassium chloride, sodium carbonate standard substance.
For so that the interference of three kinds of ions is less, the pH scope of described standard solution is between 7.3～7.5.
For so that the standard solution electrochemical environment with actual to detect sample consistent, described standard solution contains the buffering agent of specified quantitative.The consumption of described buffering agent gets final product between 7.3～7.5 for the pH scope of regulating described standard solution.The inventor finds, when adopting buffering agent to be 3-morpholine propane sulfonic acid (MOPS), the matrix of realizing solution series is unified, effectively avoids respectively organizing the evaluation distortion that the solution difference causes, and more guarantees science and validity when standard solution is estimated detection system.In addition, can also optionally adopt other acid buffer agents under the prerequisite not affecting instrument.
In order to reach higher accuracy, in the solution that contains potassium, sodium, three kinds of ions of chlorine of described variable concentrations, solvent is electronic grade ultrapure water.In addition, can also use distilled water, deionized water.
Compound method
The invention provides a kind of compound method of standard solution used for electrolytic analyzer linear calibration, take sodium chloride, potassium chloride, sodium carbonate standard substance as the reagent main body, add buffering agent (for example 3-morpholine propane sulfonic acid (MOPS)) and regulate the pH value, formulated according to desired concn with solvent (also being water, the ultrapure water of preferred electron level).
For example, compound method of the present invention is as follows: adopt 100,000/(or more accurate) electronic balance to take by weighing respectively the compound of the corresponding weight of certain component, place beaker and fully dissolve with ultrapure water, be transferred in the 1L volumetric flask.Be transferred in the same volumetric flask after the buffering agent dissolving of weighing corresponding mass, blend even allotment and be diluted to 1L with ultrapure water.
Purposes
The purposes of standard solution used for electrolytic analyzer linear calibration of the present invention is used for estimating the electrolytic analyzer linear index, can be used as simultaneously the magnitude tracing foundation of associated sample definite value.
Inventor's discovery, " standard solution used for electrolytic analyzer linear calibration " of the present invention are potassium, sodium, chlorine mixed standard solution series, are mainly used in clinical blomelicalbloodgasandelectrolrteanalyzers and calibrate/evaluation of instrument linear index when examining and determine; Under the prerequisite of getting rid of matrix effect, can be used as the standard solution that additive method carries out potassium, sodium, chlorine element definite value.Preparing process of the present invention is simple, and good stability is widely applicable, can be widely used at present at the blomelicalbloodgasandelectrolrteanalyzers with various main flow models.
Technique effect
1. study blomelicalbloodgasandelectrolrteanalyzers target detection thing, filter out suitable agent prescription, effectively avoid introducing coexisting ion to the interference of different detection systems, guarantee to detect the general applicability of solution;
2. the actual electrical chemical environment of analog detection object has increased the similarity of matrix and actual sample; In an embodiment, by adding an amount of buffering agent, realize that the matrix of solution series is unified, effectively avoid respectively organizing the evaluation distortion that the solution difference causes, guaranteed science and validity when standard solution is estimated detection system;
3. realizing on the general applicability basis of solution formula to each detection system, choosing certified reference material preparing standard solution series, guaranteeing the accuracy of standard solution value, also providing the magnitude tracing material for the associated sample definite value simultaneously.
4. good stability is easy to preserve.Plastic bottle packs in 4 ℃ of preservations 6 months, and solution appearance is unchanged, and test result is stable.
Specify such as nothing, various raw materials of the present invention all can obtain by commercially available; Or prepare according to the conventional method of this area.Unless otherwise defined or explanation, the meaning that all specialties used herein are familiar with scientific words and those skilled in the art is identical.Any method similar or impartial to described content and material all can be applicable in the inventive method in addition.
Other aspects of the present invention are because the disclosure of this paper is apparent to those skilled in the art.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used for explanation the present invention and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example is measured according to national standard usually.If there is not corresponding national standard, then carry out according to general international standard, normal condition or according to the condition that manufacturer advises.Unless otherwise indicated, otherwise all umbers are weight portion, and all number percents are weight percentage, and described polymer molecular weight is number-average molecular weight.
Embodiment 1
Standard solution used for electrolytic analyzer linear calibration is by 5 groups of solution compositions:
1) first group of solution adopt 100,000/ electronic balance take by weighing respectively sodium chloride standard substance 3.38968g, potassium chloride standard substance 0.14910g, sodium carbonate standard substance 2.22570g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.3000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
2) second group of solution adopt 100,000/ electronic balance take by weighing respectively sodium chloride standard substance 5.02608g, potassium chloride standard substance 0.29820g, sodium carbonate standard substance 2.33169g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.5000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
3) the 3rd group of solution adopt 100,000/ electronic balance take by weighing respectively sodium chloride standard substance 5.55206g, potassium chloride standard substance 0.37276g, sodium carbonate standard substance 2.38468g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.7500g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
4) the 4th group of solution adopt 100,000/ electronic balance take by weighing respectively sodium chloride standard substance 6.07805g, potassium chloride standard substance 0.44731g, sodium carbonate standard substance 2.43767g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 7.0000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
5) the 5th group of solution adopt 100,000/ electronic balance take by weighing respectively sodium chloride standard substance 7.13002g, potassium chloride standard substance 0.59641g, sodium carbonate standard substance 3.07359g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 8.7500g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
The standard solution used for electrolytic analyzer linear calibration that obtains is as shown in the table:
Above-mentioned standard solution used for electrolytic analyzer linear calibration definite value is accurate, good stability.Specifically see performance embodiment.
Embodiment 2:
Standard solution used for electrolytic analyzer linear calibration is by 5 groups of solution compositions:
1) first group of solution adopt 100,000/ electronic balance take by weighing respectively sodium chloride standard substance 3.38968g, potassium chloride standard substance 0.14910g, sodium carbonate standard substance 2.22570g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.3000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
2) second group of solution adopt 100,000/ electronic balance take by weighing respectively sodium chloride standard substance 4.47085g, potassium chloride standard substance 0.26093g, sodium carbonate standard substance 2.30520g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.5000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
3) the 3rd group of solution adopt 100,000/ electronic balance take by weighing respectively sodium chloride standard substance 5.55206g, potassium chloride standard substance 0.37276g, sodium carbonate standard substance 2.38468g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.7500g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
4) the 4th group of solution adopt 100,000/ electronic balance take by weighing respectively sodium chloride standard substance 6.63322g, potassium chloride standard substance 0.48458g, sodium carbonate standard substance 2.46417g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 7.0000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
5) the 5th group of solution adopt 100,000/ electronic balance take by weighing respectively sodium chloride standard substance 7.71440g, potassium chloride standard substance 0.59641g, sodium carbonate standard substance 2.54370g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 7.5000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
The standard solution used for electrolytic analyzer linear calibration that obtains is as shown in the table:
Above-mentioned standard solution used for electrolytic analyzer linear calibration definite value is accurate, good stability.Specifically see performance embodiment.
Performance embodiment:
(1) with the linear solution series of embodiment 1 the instrument linear index is estimated
Choose an import MEDICA EasyLyte type Na/K/Cl analyser, the domestic fast DX685 potassium, sodium and chlorine analyzers that reaches, it is qualified that selected instrument has all been calibrated, and with the serial linear standard verification solution of being prepared in the present embodiment 1 instrument carried out the linear index evaluation.Every group of concentration solution is measured 3 times at instrument, averages instrument testing result see the following form (mmol/L) after getting rid of outlier:
Take the standard solution nominal value as X, the mapping take instrument testing result mean value as Y, least square method calculates its linear equation and is:
MEDICA EasyLyte type Na/K/Cl analyser:
K：y＝0.939x+0.039，γ＝0.999；
Na：y＝0.916x+6.497，γ＝0.999；
Cl：y＝0.860x+10.351，γ＝0.999。
The fast DX685 potassium, sodium and chlorine analyzers that reaches:
K：y＝0.956x～0.052，γ＝0.999；
Na：y＝0.916x+6.470，γ＝0.999；
Cl：y＝1.067x～8.966，γ＝0.999。
Two different detection systems of three kinds of ion pairs of experimental result are carried out linear calibration, and the linearly dependent coefficient in sensing range is 0.999, and linear index is excellent, are better than in the blomelicalbloodgasandelectrolrteanalyzers industry standard requirement to linear index.This shows, calibrating liquid of the present invention possesses good applicability.
(2) magnitude tracing standard substance.
Flame photometry is that the Sodium in Serum potassium that International Federation of Clinical Chemistry (IFCC) is recommended detects reference method.Therefore, series standard solution is calibration solution in the present embodiment 1, and flame photometry is to serum quality-control product definite value.
Standard solution used for electrolytic analyzer linear calibration series is diluted respectively 1000 times and 100 times of typical curve series as measurement of sodium and potassium.The serum sample equal proportion is diluted identical multiple and is used for measuring corresponding element.
Typical curve and the sample results of flame luminosity instrumentation potassium see the following form:
Pattern detection result
| Sample number into spectrum | 1# | 2# | 3# | 4# | 5# |
| Signal intensity | 36.8 | 36.6 | 36.7 | 36.6 | 36.6 |
| Concentration testing result (mmol/L) | 3.08 | 3.06 | 3.07 | 3.06 | 3.06 |
Typical curve and the sample results of flame luminosity instrumentation sodium see the following form:
Pattern detection result
| Sample number into spectrum | 1# | 2# | 3# | 4# | 5# |
| Signal intensity | 45.5 | 45.1 | 45.2 | 45.3 | 45.4 |
| Concentration testing result (mmol/L) | 123.7 | 122.6 | 122.9 | 123.2 | 123.5 |
According to above form as can be known, the quality-control product testing result all in given term of reference, the measurement result accurate and effective.
The above only is preferred embodiment of the present invention, be not to limit essence technology contents scope of the present invention, essence technology contents of the present invention is broadly to be defined in the claim scope of application, any technology entity or method that other people finish, if defined identical with the claim scope of application, also or a kind of change of equivalence, all will be regarded as being covered by among this claim scope.
All quote in this application as a reference at all documents that the present invention mentions, just as each piece document is quoted separately as a reference.Should be understood that in addition after having read foregoing of the present invention, those skilled in the art can make various changes or modifications the present invention, these equivalent form of values fall within the application's appended claims limited range equally.
Claims (7)
1. standard solution used for electrolytic analyzer linear calibration, it is characterized in that, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of at least 5 group variable concentrations, the described concentration range that contains the solution of potassium, sodium, three kinds of ions of chlorine is respectively: potassium (K) 2mmol/L～8mmol/L, sodium (Na) 100mmol/L～180mmol/L, chlorine (Cl) 60mmol/L～140mmol/L;
The pH scope of described standard solution is between 7.3～7.5;
Described standard solution is take sodium chloride, potassium chloride, sodium carbonate standard substance as the reagent main body, adds buffering agent and regulates the pH value between 7.3～7.5, and is formulated according to desired concn;
Described buffering agent is 3-morpholine propane sulfonic acid (MOPS).
2. standard solution as claimed in claim 1 is characterized in that, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5～8 groups of variable concentrations.
3. standard solution as claimed in claim 1 is characterized in that, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5 groups of variable concentrations.
4. standard solution as claimed in claim 3 is characterized in that, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5 groups of variable concentrations, and the concentration of these 5 groups of solution with the mmol/L score is not:
Potassium ion: X
1, X
2, X
3, X
4, X
5
Sodion: Y
1, Y
2, Y
3, Y
4, Y
5
Chlorion: Z
1, Z
2, Z
3, Z
4, Z
5
Wherein,
X
1＝2～4、X
2＝3～5、X
3＝4～6、X
4＝5～7、X
5＝6～8；
Y
1＝100～120、Y
2＝110～130、Y
3＝130～150、Y
4＝150～170、Y
5＝160～180；
Z
1＝60～80、Z
2＝70～90、Z
3＝90～110、Z
4＝110～130、Z
5＝120～140；
And meet the following conditions: X
1＜X
2＜X
3＜X
4＜X
5Y
1＜Y
2＜Y
3＜Y
4＜Y
5Z
1＜Z
2＜Z
3＜Z
4＜Z
5
5. standard solution as claimed in claim 4 is characterized in that, described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of 5 groups of variable concentrations, and the concentration of these 5 groups of solution with the mmol/L score is not:
Potassium ion: 2,4,5,6,8;
Sodion: 100,130,140,150,180;
Chlorion: 60,90,100,110,140.
6. standard solution as claimed in claim 1 is characterized in that, the solvent of described standard solution is electronic grade ultrapure water.
7. the compound method of a standard solution used for electrolytic analyzer linear calibration as claimed in claim 1, it is characterized in that, take sodium chloride, potassium chloride, sodium carbonate standard substance as the reagent main body, add buffering agent and regulate the pH value between 7.3～7.5, described buffering agent is 3-morpholine propane sulfonic acid (MOPS); According to the formulated standard solution of desired concn:
Described standard solution is by the solution composition that contains potassium, sodium, three kinds of ions of chlorine of at least 5 group variable concentrations, the described concentration range that contains the solution of potassium, sodium, three kinds of ions of chlorine is respectively: potassium (K) 2mmol/L～8mmol/L, sodium (Na) 100mmol/L～180mmol/L, chlorine (Cl) 60mmol/L～140mmol/L;
The pH scope of described standard solution is between 7.3～7.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910196844 CN101672815B (en) | 2009-09-29 | 2009-09-29 | Standard solution used for electrolytic analyzer linear calibration |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200910196844 CN101672815B (en) | 2009-09-29 | 2009-09-29 | Standard solution used for electrolytic analyzer linear calibration |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101672815A CN101672815A (en) | 2010-03-17 |
| CN101672815B true CN101672815B (en) | 2013-03-06 |
Family
ID=42020132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200910196844 Active CN101672815B (en) | 2009-09-29 | 2009-09-29 | Standard solution used for electrolytic analyzer linear calibration |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101672815B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102809590B (en) * | 2011-06-02 | 2014-10-08 | 深圳中科优瑞医疗科技有限公司 | Lithium-ion test method of electrolyte analyzer |
| CN102998351B (en) * | 2012-12-12 | 2015-04-15 | 广州东林生物科技有限公司 | Auxiliary reagent for ISE module of biochemical analyzer |
| CN105044159B (en) * | 2015-09-09 | 2018-05-22 | 深圳市新产业生物医学工程股份有限公司 | The test method of blomelicalbloodgasandelectrolrteanalyzers |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4877492A (en) * | 1986-01-11 | 1989-10-31 | Horiba, Ltd. | Method of measuring electrolyte in blood and urine by electrode method |
-
2009
- 2009-09-29 CN CN 200910196844 patent/CN101672815B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4877492A (en) * | 1986-01-11 | 1989-10-31 | Horiba, Ltd. | Method of measuring electrolyte in blood and urine by electrode method |
Non-Patent Citations (4)
| Title |
|---|
| JP特开2006-98406A 2006.04.13 |
| JP特开平7-325063A 1995.12.12 |
| 蒋雪萍.电解质分析仪校准方法的探讨.《上海计量测试》.2008,(第206期),13-14. * |
| 魏力强等.对离子选择电极法电解质分析仪性能评价指标的研究.《现代检验医学杂志》.2007,第22卷(第5期),22-24. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101672815A (en) | 2010-03-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Maccà | Response time of ion-selective electrodes: Current usage versus IUPAC recommendations | |
| Giordano et al. | Performance of seven blood glucose testing systems at high altitude | |
| CN108680622A (en) | Packed cell volume measures and the method for correction in a kind of electrochemica biological sensor | |
| CN103424443A (en) | Ion-selective electrode method for measuring fluoride content in grains, vegetables and fruits | |
| Kim et al. | PVC membrane-based portable ion analyzer for hydroponic and water monitoring | |
| CN101144824B (en) | Total bilirubin determination reagent kit | |
| Hawkins | Evaluation of roche accu-chek go and medisense optium blood glucose meters | |
| CN101672815B (en) | Standard solution used for electrolytic analyzer linear calibration | |
| JP2018510956A (en) | Improved magnesium ion selective membrane | |
| Cao et al. | Determination of clinically acceptable cut-offs for hemolysis index: An application of bootstrap method using real-world data | |
| Maj-Zurawska et al. | Selectivity coefficients of ion-selective magnesium electrodes used for simultaneous determination of magnesium and calcium ions | |
| D'Orazio et al. | Accuracy of commercial blood gas analyzers for monitoring ionized calcium at low concentrations | |
| CN100373153C (en) | Method for measuring general quantity of released gas during cable material combustion | |
| EP2316017B1 (en) | Use of polyoxyethylene stearyl ether nonionic surfactants with magnesium ion selective electrodes | |
| CN102866197A (en) | Method for quickly detecting concentrations of various electrolytes by ion-selective electrodes | |
| CN114544734A (en) | Reagent matched with electrolyte module of Beckmann AU series analyzer | |
| CN103512933A (en) | Method for measuring clenbuterol residue amount | |
| CN113916812A (en) | Method for measuring total nitrogen concentration in fresh water | |
| CN102998351B (en) | Auxiliary reagent for ISE module of biochemical analyzer | |
| Ben Chioma et al. | A comparative study on the measurement of ph of water, using ph metre and water testing kit [testube method] in port harcourt | |
| Schlueter et al. | Glucose analytical comparability evaluation of the YSI 2300 STAT Plus™ and YSI 2900D biochemistry analyzers | |
| Mueller et al. | Towards an automated, standardized protocol for determination of equilibrium potential of ion-selective electrodes | |
| Obstfeld et al. | Report of the International Council for Standardization in Haematology working group for standardization of reticulocyte parameters | |
| CN107957477A (en) | A kind of method using non-aqueous titration measure posaconazole content | |
| CN102297844B (en) | Detection method of sodion and kit |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |