CN101672815A - Standard solution used for electrolytic analyzer linear calibration - Google Patents
Standard solution used for electrolytic analyzer linear calibration Download PDFInfo
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- CN101672815A CN101672815A CN 200910196844 CN200910196844A CN101672815A CN 101672815 A CN101672815 A CN 101672815A CN 200910196844 CN200910196844 CN 200910196844 CN 200910196844 A CN200910196844 A CN 200910196844A CN 101672815 A CN101672815 A CN 101672815A
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Abstract
The invention provides a standard solution used for electrolytic analyzer linear calibration, which is composed of five groups of different-concentration solutions containing potassium ion, sodion andchloridion. The concentration range of the three ions in the standard solution is respectively as follows: 2-8mmol/L of K, 100-180mmol/L of Na, 60-140mmol/L of Cl, and the pH range of the solutions is 7.3-7.5. The invention also discloses the preparing method of the standard solution; the invention provides a widely-used standard solution used for electrolytic analyzer linear calibration and provides guarantee for accurately evaluating instrument performance index.
Description
Technical field
The present invention relates to the clinical examination calibrating of blomelicalbloodgasandelectrolrteanalyzers and the transmission of quantity value field of metrology and measurement, particularly a kind of standard solution used for electrolytic analyzer linear calibration.
Background technology
In medical diagnosis on disease, the doctor often will measure the electrolyte concentration of body fluid such as patients'blood, and the gained data are as the basis for estimation to disease.Wherein, flame photometry is that sodium potassium detects reference method in the serum recommended of International Federation of Clinical Chemistry (IFCC).But, the complicated operation of flame photometer, and contaminated air, analysis time is long, and can only measure the concentration of a certain ion among the human electrolyte each time.Therefore this method is progressively replaced by blomelicalbloodgasandelectrolrteanalyzers institute.
At present, blomelicalbloodgasandelectrolrteanalyzers is mainly used in the electrolyte ingredient analysis in clinical treatment human body serum and the urine, is main detected object with potassium, sodium, chlorion.As an important step in the clinical examination quality control, standard committee of U.S. clinical labororatory (NCCLS) has issued EP～6P normative document " quantitative detecting method linear evaluation ", and the linear evaluation of detection by quantitative is claimed.Linear index is the important content of clinical examination mass of system control, also is the important accurately and reliably assurance of blomelicalbloodgasandelectrolrteanalyzers testing result.
The linear calibration that blomelicalbloodgasandelectrolrteanalyzers is used is with standard solution and be different from the linear calibration standard solution of common meaning.Because the principle of the blomelicalbloodgasandelectrolrteanalyzers that uses in the Clinical Test Lab is based on the specificly-response of ion-selective electrode to specific ion at present, yet the electrode performance that the instrument of each producer's different model uses is all variant, promptly the influential ion of its selectivity is had nothing in common with each other.If in calibrating solution, introduced the ion that ion-selective electrode is had response, will bring influence to the accuracy of evaluation result.
Therefore, need be a kind of specially at standard solution used for electrolytic analyzer linear calibration, for accurate evaluation appts performance index are given security.
The linear solution that has blomelicalbloodgasandelectrolrteanalyzers when calibrating of bibliographical information to use at present is to analyze pure or other chemical reagent of higher level is directly prepared mostly.Such solution in use has certain limitation, the main problem that has following two aspects:
1. solution concentration does not have definite value accurately, lacks the foundation of magnitude tracing.Conventional chemical reagent is the minimum content of mark sample in explanation only, and this solution that just causes preparing can not provide accurate value, can't carry out transmission of quantity value and trace to the source;
2. coexisting ion disturbs analytical approach.Because each detection system causes also having nothing in common with each other to measuring noisy ion for different ions difference optionally.Therefore the coexisting ion beyond the detected object of introducing in process for preparation will inevitably influence the general applicability of solution.For example, the acetate ion (Ac ~) of coexistence is introduced in the daily demarcation of part autogamy instrument at present in process for preparation with reagent.Show that according to test the chlorion working electrode of part model instrument is to Ac ~ have response, and increase the chlorine ion concentration indicating value with Ac ~ concentration and increase, cause test result to be false positive.
3. do not possess broad applicability.
In sum, this area lacks a kind of standard solution used for electrolytic analyzer linear calibration with broad applicability, for accurate evaluation appts performance index are given security.
Therefore, this area presses for a kind of linear calibration standard solution that accurate evaluation result is provided and has broad applicability at blomelicalbloodgasandelectrolrteanalyzers.
Summary of the invention
First purpose of the present invention is to obtain a kind of standard solution used for electrolytic analyzer linear calibration with broad applicability, for accurate evaluation appts performance index are given security.
Second purpose of the present invention is to obtain a kind of compound method with standard solution used for electrolytic analyzer linear calibration of broad applicability, for accurate evaluation appts performance index are given security.
In a first aspect of the present invention, a kind of standard solution used for electrolytic analyzer linear calibration is provided, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5 group variable concentrations at least, the described concentration range that contains potassium, sodium, three kinds of ion solution of chlorine is respectively: potassium (K) 2mmol/L～8mmol/L, sodium (Na) 100mmol/L～180mmol/L, chlorine (Cl) 60mmol/L～140mmol/L;
The pH scope of described standard solution is between 7.3～7.5.
In a specific embodiment of the present invention, the pH scope of described standard solution is regulated by 3-morpholine propane sulfonic acid (MOPS) buffering agent.
In a specific embodiment of the present invention, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5～8 groups of variable concentrations.
In a specific embodiment of the present invention, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5 groups of variable concentrations.
In a specific embodiment of the present invention, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5 groups of variable concentrations, and the concentration of these 5 groups of solution with the mmol/L score is not:
Potassium ion: X
1, X
2, X
3, X
4, X
5
Sodion: Y
1, Y
2, Y
3, Y
4, Y
5
Chlorion: Z
1, Z
2, Z
3, Z
4, Z
5
Wherein,
X
1＝2～4、X
2＝3～5、X
3＝4～6、X
4＝5～7、X
5＝6～8；
Y
1＝100～120、Y
2＝110～130、Y
3＝130～150、Y
4＝150～170、Y
5＝160～180；
Z
1＝60～80、Z
2＝70～90、Z
3＝90～110、Z
4＝110～130、Z
5＝120～140；
And meet the following conditions: X
1＜X
2＜X
3＜X
4＜X
5Y
1＜Y
2＜Y
3＜Y
4＜Y
5Z
1＜Z
2＜Z
3＜Z
4＜Z
5
In a specific embodiment of the present invention, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5 groups of variable concentrations, and the concentration of these 5 groups of solution with the mmol/L score is not:
Potassium ion: 2,4,5,6,8;
Sodion: 100,130,140,150,180;
Chlorion: 60,90,100,110,140.
In a specific embodiment of the present invention, described to contain potassium, sodium, three kinds of ion solution of chlorine formulated by desired concn by sodium chloride, potassium chloride, sodium carbonate standard substance.
In a specific embodiment of the present invention, the solvent of described standard solution is the electron level ultrapure water.
A second aspect of the present invention provides a kind of compound method of standard solution used for electrolytic analyzer linear calibration of the present invention, it is the reagent main body with sodium chloride, potassium chloride, sodium carbonate standard substance, add buffering agent and regulate required pH value, formulated according to desired concn.
In a specific embodiment of the present invention, described buffering agent is a 3-morpholine propane sulfonic acid (MOPS).
Embodiment
The inventor, has obtained at standard solution used for electrolytic analyzer linear calibration by improving preparation technology through extensive and deep research, for accurate evaluation appts performance index are given security.Finished the present invention on this basis.
Unless otherwise defined or explanation, same meanings of being familiar with of all specialties used herein and scientific words and those skilled in the art.Any in addition method similar or impartial to described content and material all can be applicable in the inventive method.All reagent are commercially available getting.Below describe in detail to various aspects of the present invention:
Standard solution used for electrolytic analyzer linear calibration
Serial solution of the present invention is at least 5 groups, and wherein potassium, sodium, chlorine ion concentration are respectively: potassium (K) 2mmol/L～8mmol/L, sodium (Na) 100mmol/L～180mmol/L, chlorine (Cl) 60mmol/L～140mmol/L.
Particularly, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5～8 groups of variable concentrations.If the group number very little, then the effect of linear calibration is relatively poor even can't reach requirement; If the group number increases, also can reach goal of the invention of the present invention, but cost strengthens.
More preferably, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5 groups of variable concentrations.More specifically, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5 groups of variable concentrations, and its concentration is as shown in the table respectively: (unit is mmol/l)
| First group | Second group | The 3rd group | The 4th group | The 5th group | |
| Potassium | ??X 1 | ??X 2 | ??X 3 | ??X 4 | ??X 5 |
| Sodium | ??Y 1 | ??Y 2 | ??Y 3 | ??Y 4 | ??Y 5 |
| Chlorine | ??Z 1 | ??Z 2 | ??Z 3 | ??Z 4 | ??Z 5 |
Wherein:
The span of 5 groups of potassium, sodium, three kinds of ions of chlorine is as shown in the table: (unit is mmol/l)
| First group | Second group | The 3rd group | The 4th group | The 5th group | |
| Potassium | ??2～4 | ??3～5 | ??4～6 | ??5～7 | ??6～8 |
| Sodium | ??100～120 | ??110～130 | ??130～150 | ??150～170 | ??160～180 |
| Chlorine | ??60～80 | ??70～90 | ??90～110 | ??110～130 | ??120～140 |
The span of 5 groups of potassium, sodium, three kinds of ions of chlorine and meeting the following conditions: X
1＜X
2＜X
3＜X
4＜X
5Y
1＜Y
2＜Y
3＜Y
4＜Y
5Z
1＜Z
2＜Z
3＜Z
4＜Z
5
More preferably, meet the following conditions:
The interval of the concentration of potassium (also is X
n～X
N-1, be between 1～2 n=5);
The interval of the concentration of sodium (also is Y
n～Y
N-1, be between 10～30 n=5);
The interval of the concentration of chlorine (also is Z
n～Z
N-1, be between 10～30 n=5);
More specifically, the span of 5 groups of potassium, sodium, three kinds of ions of chlorine is as shown in the table:
It is described that to contain potassium, sodium, three kinds of ion solution of chlorine preferably formulated by sodium chloride, potassium chloride, sodium carbonate standard substance.
In order to make the interference of three kinds of ions littler, the pH scope of described standard solution is between 7.3～7.5.
In order to make the standard solution electrochemical environment consistent with the actual detected sample, described standard solution contains the buffering agent of specified quantitative.The consumption of described buffering agent gets final product between 7.3～7.5 for the pH scope of regulating described standard solution.The inventor finds, when adopting buffering agent to be 3-morpholine propane sulfonic acid (MOPS), the matrix of realizing solution series is unified, effectively avoids respectively organizing the evaluation distortion that difference causes between the solution, guarantees science and validity when standard solution is estimated detection architecture more.In addition, can also optionally adopt other acid buffer agents under the prerequisite not influencing instrument.
In order to reach higher accuracy, the containing in potassium, sodium, three kinds of ion solution of chlorine of described variable concentrations, solvent is the electron level ultrapure water.In addition, can also use distilled water, deionized water.
Compound method
The invention provides a kind of compound method of standard solution used for electrolytic analyzer linear calibration, with sodium chloride, potassium chloride, sodium carbonate standard substance is the reagent main body, add buffering agent (for example 3-morpholine propane sulfonic acid (MOPS)) and regulate the pH value, formulated with solvent (also being water, the ultrapure water of preferred electron level) according to desired concn.
For example, compound method of the present invention is as follows: adopt 100,000/(or more accurate) electronic balance to take by weighing the compound of the corresponding weight of certain component respectively, place beaker and fully dissolve with ultrapure water, be transferred in the 1L volumetric flask.Be transferred in the same volumetric flask after the buffering agent dissolving of weighing corresponding mass, blend even allotment and be diluted to 1L with ultrapure water.
Purposes
The purposes of standard solution used for electrolytic analyzer linear calibration of the present invention is used to estimate the electrolytic analyzer linear index, can be used as the magnitude tracing foundation of associated sample definite value simultaneously.
The inventor finds that " standard solution used for electrolytic analyzer linear calibration " of the present invention be potassium, sodium, chlorine mixed standard solution series, instrument linearity evaluation of indexes when being mainly used in clinical blomelicalbloodgasandelectrolrteanalyzers calibration/calibrating; Under the prerequisite of getting rid of matrix effect, can be used as the standard solution that additive method carries out potassium, sodium, chlorine element definite value.Preparing process of the present invention is simple, and good stability is widely applicable, can be widely used at present at the blomelicalbloodgasandelectrolrteanalyzers with various main flow models.
Technique effect
1. study blomelicalbloodgasandelectrolrteanalyzers target detection thing, filter out suitable agent prescription, effectively avoid introducing the interference of coexisting ion, guarantee to detect the general applicability of solution different detection systems;
2. the actual electrical chemical environment of analog detection object has increased the similarity of matrix and actual sample; In an embodiment, by adding an amount of buffering agent, realize that the matrix of solution series is unified, effectively avoid respectively organizing the evaluation distortion that difference causes between the solution, guaranteed science and validity when standard solution is estimated detection architecture;
3. realizing choosing certified reference material preparing standard solution series on the general applicability basis of solution formula to each detection system, guaranteed the accuracy of standard solution value, also provide the magnitude tracing material simultaneously for the associated sample definite value.
4. good stability is easy to preserve.Plastic bottle packs in 4 ℃ of preservations 6 months, the solution appearance no change, and test result is stable.
Specify as nothing, various raw materials of the present invention all can obtain by commercially available; Or prepare according to the conventional method of this area.Unless otherwise defined or explanation, same meanings of being familiar with of all specialties used herein and scientific words and those skilled in the art.Any in addition method similar or impartial to described content and material all can be applicable in the inventive method.
Other aspects of the present invention are because the disclosure of this paper is conspicuous to those skilled in the art.
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example is measured according to national standard usually.If there is not a corresponding national standards, then carry out according to general international standard, normal condition or according to the condition that manufacturer advises.Unless otherwise indicated, otherwise all umbers are weight portion, and all number percents are weight percentage, and described polymer molecular weight is a number-average molecular weight.
Embodiment 1
Standard solution used for electrolytic analyzer linear calibration is by 5 groups of solution compositions:
1) first group of solution adopt 100,000/ electronic balance take by weighing sodium chloride standard substance 3.38968g respectively, potassium chloride standard substance 0.14910g, sodium carbonate standard substance 2.22570g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.3000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
2) second group of solution adopt 100,000/ electronic balance take by weighing sodium chloride standard substance 5.02608g respectively, potassium chloride standard substance 0.29820g, sodium carbonate standard substance 2.33169g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.5000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
3) the 3rd group of solution adopt 100,000/ electronic balance take by weighing sodium chloride standard substance 5.55206g respectively, potassium chloride standard substance 0.37276g, sodium carbonate standard substance 2.38468g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.7500g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
4) the 4th group of solution adopt 100,000/ electronic balance take by weighing sodium chloride standard substance 6.07805g respectively, potassium chloride standard substance 0.44731g, sodium carbonate standard substance 2.43767g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 7.0000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
5) the 5th group of solution adopt 100,000/ electronic balance take by weighing sodium chloride standard substance 7.13002g respectively, potassium chloride standard substance 0.59641g, sodium carbonate standard substance 3.07359g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 8.7500g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
The standard solution used for electrolytic analyzer linear calibration that obtains is as shown in the table:
Above-mentioned standard solution used for electrolytic analyzer linear calibration definite value is accurate, good stability.Specifically see performance embodiment.
Embodiment 2:
Standard solution used for electrolytic analyzer linear calibration is by 5 groups of solution compositions:
1) first group of solution adopt 100,000/ electronic balance take by weighing sodium chloride standard substance 3.38968g respectively, potassium chloride standard substance 0.14910g, sodium carbonate standard substance 2.22570g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.3000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
2) second group of solution adopt 100,000/ electronic balance take by weighing sodium chloride standard substance 4.47085g respectively, potassium chloride standard substance 0.26093g, sodium carbonate standard substance 2.30520g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.5000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
3) the 3rd group of solution adopt 100,000/ electronic balance take by weighing sodium chloride standard substance 5.55206g respectively, potassium chloride standard substance 0.37276g, sodium carbonate standard substance 2.38468g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 6.7500g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
4) the 4th group of solution adopt 100,000/ electronic balance take by weighing sodium chloride standard substance 6.63322g respectively, potassium chloride standard substance 0.48458g, sodium carbonate standard substance 2.46417g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 7.0000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
5) the 5th group of solution adopt 100,000/ electronic balance take by weighing sodium chloride standard substance 7.71440g respectively, potassium chloride standard substance 0.59641g, sodium carbonate standard substance 2.54370g places beaker, fully dissolves with ultrapure water, shifts and puts in the 1L volumetric flask.Weighing 3-morpholine propane sulfonic acid 7.5000g places beaker, fully dissolves with ultrapure water, is transferred in the same volumetric flask, blends even allotment and is diluted to 1L with ultrapure water.
The standard solution used for electrolytic analyzer linear calibration that obtains is as shown in the table:
Above-mentioned standard solution used for electrolytic analyzer linear calibration definite value is accurate, good stability.Specifically see performance embodiment.
Performance embodiment:
(1) with the linear solution series of embodiment 1 the instrument linear index is estimated
Choose an import MEDICA EasyLyte type Na/K/Cl analyser, the homemade fast DX685 potassium, sodium and chlorine analyzers that reaches, it is qualified that selected instrument has all been calibrated, and with the serial linear standard verification solution of being prepared in the present embodiment 1 instrument carried out the linear index evaluation.Every group of concentration solution is measured on instrument 3 times, averages after the eliminating outlier, and instrument detecting the results are shown in following table (mmol/L):
With the standard solution nominal value is X, with instrument detecting as a result mean value be Y mapping, least square method calculates its linear equation and is:
MEDICA EasyLyte type Na/K/Cl analyser:
K：y＝0.939x+0.039，γ＝0.999；
Na：y＝0.916x+6.497，γ＝0.999；
Cl：y＝0.860x+10.351，γ＝0.999。
The fast DX685 potassium, sodium and chlorine analyzers that reaches:
K：y＝0.956x～0.052，γ＝0.999；
Na：y＝0.916x+6.470，γ＝0.999；
Cl：y＝1.067x～8.966，γ＝0.999。
Two different detection systems of three kinds of ion pairs of experimental result are carried out linear calibration, and the linearly dependent coefficient in sensing range is 0.999, and the linear index excellence is better than in the blomelicalbloodgasandelectrolrteanalyzers industry standard requirement to linear index.This shows that calibrating liquid of the present invention possesses favorable applicability.
(2) magnitude tracing standard substance.
Flame photometry is that sodium potassium detects reference method in the serum recommended of International Federation of Clinical Chemistry (IFCC).Therefore, serve as calibration solution with series standard solution in the present embodiment 1, flame photometry is given serum quality-control product definite value.
Standard solution used for electrolytic analyzer linear calibration series is diluted 1000 times and 100 times of typical curve series as mensuration sodium and potassium respectively.The serum sample equal proportion is diluted identical multiple and is used to measure corresponding element.
The typical curve and the sample results of flame luminosity instrumentation potassium see the following form:
Pattern detection result
| Sample number into spectrum | ??1# | ??2# | ??3# | ??4# | ??5# |
| Signal intensity | ??36.8 | ??36.6 | ??36.7 | ??36.6 | ??36.6 |
| Concentration testing result (mmol/L) | ??3.08 | ??3.06 | ??3.07 | ??3.06 | ??3.06 |
The typical curve and the sample results of flame luminosity instrumentation sodium see the following form:
Pattern detection result
| Sample number into spectrum | ??1# | ??2# | ??3# | ??4# | ??5# |
| Signal intensity | ??45.5 | ??45.1 | ??45.2 | ??45.3 | ??45.4 |
| Concentration testing result (mmol/L) | ??123.7 | ??122.6 | ??122.9 | ??123.2 | ??123.5 |
According to above form as can be known, the quality-control product testing result all in given term of reference, the measurement result accurate and effective.
The above only is preferred embodiment of the present invention, be not in order to limit essence technology contents scope of the present invention, essence technology contents of the present invention is broadly to be defined in the claim scope of application, any technology entity or method that other people finish, if it is defined identical with the claim scope of application, also or a kind of change of equivalence, all will be regarded as being covered by among this claim scope.
All quote in this application as a reference at all documents that the present invention mentions, just quoted as a reference separately as each piece document.Should be understood that in addition after having read foregoing of the present invention, those skilled in the art can make various changes or modifications the present invention, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
Claims (10)
1. standard solution used for electrolytic analyzer linear calibration, it is characterized in that, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5 group variable concentrations at least, the described concentration range that contains potassium, sodium, three kinds of ion solution of chlorine is respectively: potassium (K) 2mmol/L～8mmol/L, sodium (Na) 100mmol/L～180mmol/L, chlorine (Cl) 60mmol/L～140mmol/L;
The pH scope of described standard solution is between 7.3～7.5.
2. standard solution as claimed in claim 1 is characterized in that, the pH scope of described standard solution is regulated by 3-morpholine propane sulfonic acid (MOPS) buffering agent.
3. standard solution as claimed in claim 1 is characterized in that, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5～8 groups of variable concentrations.
4. standard solution as claimed in claim 1 is characterized in that, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5 groups of variable concentrations.
5. standard solution as claimed in claim 4 is characterized in that, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5 groups of variable concentrations, and the concentration of these 5 groups of solution with the mmol/L score is not:
Potassium ion: X
1, X
2, X
3, X
4, X
5
Sodion: Y
1, Y
2, Y
3, Y
4, Y
5
Chlorion: Z
1, Z
2, Z
3, Z
4, Z
5
Wherein,
X
1＝2～4、X
2＝3～5、X
3＝4～6、X
4＝5～7、X
5＝6～8；
Y
1＝100～120、Y
2＝110～130、Y
3＝130～150、Y
4＝150～170、Y
5＝160～180；
Z
1＝60～80、Z
2＝70～90、Z
3＝90～110、Z
4＝110～130、Z
5＝120～140；
And meet the following conditions: X
1＜X
2＜X
3＜X
4＜X
5Y
1＜Y
2＜Y
3＜Y
4＜Y
5Z
1＜Z
2＜Z
3＜Z
4＜Z
5
6. standard solution as claimed in claim 5 is characterized in that, described standard solution is made up of the contain potassium, sodium, three kinds of ion solution of chlorine of 5 groups of variable concentrations, and the concentration of these 5 groups of solution with the mmol/L score is not:
Potassium ion: 2,4,5,6,8;
Sodion: 100,130,140,150,180;
Chlorion: 60,90,100,110,140.
7. standard solution as claimed in claim 1 is characterized in that, described to contain potassium, sodium, three kinds of ion solution of chlorine formulated by desired concn by sodium chloride, potassium chloride, sodium carbonate standard substance.
8. standard solution as claimed in claim 1 is characterized in that, the solvent of described standard solution is the electron level ultrapure water.
9. the compound method of a standard solution used for electrolytic analyzer linear calibration as claimed in claim 1, it is characterized in that, with sodium chloride, potassium chloride, sodium carbonate standard substance is the reagent main body, adds buffering agent and regulates the pH value between 7.3～7.5, formulated according to desired concn.
10. method as claimed in claim 9 is characterized in that, described buffering agent is a 3-morpholine propane sulfonic acid (MOPS).
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102809590A (en) * | 2011-06-02 | 2012-12-05 | 深圳中科优瑞医疗科技有限公司 | Lithium-ion test method of electrolyte analyzer |
| CN102998351A (en) * | 2012-12-12 | 2013-03-27 | 广州东林生物科技有限公司 | Auxiliary reagent for ISE module of biochemical analyzer |
| CN105044159A (en) * | 2015-09-09 | 2015-11-11 | 深圳市新产业生物医学工程股份有限公司 | Testing method for electrolyte analyzer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JPH0627723B2 (en) * | 1986-01-11 | 1994-04-13 | 株式会社堀場製作所 | Electrode method for measuring electrolytes in blood or urine |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102809590A (en) * | 2011-06-02 | 2012-12-05 | 深圳中科优瑞医疗科技有限公司 | Lithium-ion test method of electrolyte analyzer |
| CN102809590B (en) * | 2011-06-02 | 2014-10-08 | 深圳中科优瑞医疗科技有限公司 | Lithium-ion test method of electrolyte analyzer |
| CN102998351A (en) * | 2012-12-12 | 2013-03-27 | 广州东林生物科技有限公司 | Auxiliary reagent for ISE module of biochemical analyzer |
| CN102998351B (en) * | 2012-12-12 | 2015-04-15 | 广州东林生物科技有限公司 | Auxiliary reagent for ISE module of biochemical analyzer |
| CN105044159A (en) * | 2015-09-09 | 2015-11-11 | 深圳市新产业生物医学工程股份有限公司 | Testing method for electrolyte analyzer |
| CN105044159B (en) * | 2015-09-09 | 2018-05-22 | 深圳市新产业生物医学工程股份有限公司 | The test method of blomelicalbloodgasandelectrolrteanalyzers |
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