CN101663404B - 用于定量测定小而密ldl的试剂 - Google Patents
用于定量测定小而密ldl的试剂 Download PDFInfo
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- CN101663404B CN101663404B CN2008800130830A CN200880013083A CN101663404B CN 101663404 B CN101663404 B CN 101663404B CN 2008800130830 A CN2008800130830 A CN 2008800130830A CN 200880013083 A CN200880013083 A CN 200880013083A CN 101663404 B CN101663404 B CN 101663404B
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- Prior art keywords
- small
- dense ldl
- cholesterol
- tensio
- active agent
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- 108010007622 LDL Lipoproteins Proteins 0.000 title abstract description 215
- 102000007330 LDL Lipoproteins Human genes 0.000 title abstract description 215
- 239000003153 chemical reaction reagent Substances 0.000 title abstract description 67
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 116
- 238000000034 method Methods 0.000 claims abstract description 74
- 102000004895 Lipoproteins Human genes 0.000 claims abstract description 71
- 108090001030 Lipoproteins Proteins 0.000 claims abstract description 71
- 235000012000 cholesterol Nutrition 0.000 claims abstract description 55
- 102000000019 Sterol Esterase Human genes 0.000 claims abstract description 30
- 108010055297 Sterol Esterase Proteins 0.000 claims abstract description 30
- 239000004094 surface-active agent Substances 0.000 claims abstract description 23
- 239000013543 active substance Substances 0.000 claims description 93
- 238000012207 quantitative assay Methods 0.000 claims description 55
- -1 polyoxyethylene Polymers 0.000 claims description 42
- 102000004190 Enzymes Human genes 0.000 claims description 37
- 108090000790 Enzymes Proteins 0.000 claims description 37
- 238000008214 LDL Cholesterol Methods 0.000 claims description 27
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims description 26
- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 24
- 108010013563 Lipoprotein Lipase Proteins 0.000 claims description 20
- 102000043296 Lipoprotein lipases Human genes 0.000 claims description 20
- 238000005259 measurement Methods 0.000 claims description 18
- 125000000217 alkyl group Chemical group 0.000 claims description 13
- 150000005215 alkyl ethers Chemical class 0.000 claims description 12
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 10
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims description 10
- 150000001408 amides Chemical class 0.000 claims description 10
- 229960003237 betaine Drugs 0.000 claims description 10
- 229920001992 poloxamer 407 Polymers 0.000 claims description 10
- 102000016938 Catalase Human genes 0.000 claims description 8
- 108010053835 Catalase Proteins 0.000 claims description 8
- DVEKCXOJTLDBFE-UHFFFAOYSA-N n-dodecyl-n,n-dimethylglycinate Chemical compound CCCCCCCCCCCC[N+](C)(C)CC([O-])=O DVEKCXOJTLDBFE-UHFFFAOYSA-N 0.000 claims description 7
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 claims description 6
- 125000005037 alkyl phenyl group Chemical group 0.000 claims description 6
- 125000000118 dimethyl group Chemical group [H]C([H])([H])* 0.000 claims description 6
- 150000003973 alkyl amines Chemical class 0.000 claims description 4
- 239000003093 cationic surfactant Substances 0.000 claims description 4
- LQZZUXJYWNFBMV-UHFFFAOYSA-N dodecan-1-ol Chemical compound CCCCCCCCCCCCO LQZZUXJYWNFBMV-UHFFFAOYSA-N 0.000 claims description 4
- MTNDZQHUAFNZQY-UHFFFAOYSA-N imidazoline Chemical compound C1CN=CN1 MTNDZQHUAFNZQY-UHFFFAOYSA-N 0.000 claims description 4
- 229940094506 lauryl betaine Drugs 0.000 claims description 4
- 229910021653 sulphate ion Inorganic materials 0.000 claims description 4
- 230000009471 action Effects 0.000 claims description 3
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims description 3
- 108091008012 small dense LDL Proteins 0.000 abstract description 2
- 230000002000 scavenging effect Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 description 45
- 238000006243 chemical reaction Methods 0.000 description 28
- 230000000694 effects Effects 0.000 description 11
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 10
- 241000047703 Nonion Species 0.000 description 10
- 230000009257 reactivity Effects 0.000 description 10
- 230000004044 response Effects 0.000 description 10
- 238000006911 enzymatic reaction Methods 0.000 description 9
- 102000003992 Peroxidases Human genes 0.000 description 8
- 108010023417 cholesterol dehydrogenase Proteins 0.000 description 8
- 108040007629 peroxidase activity proteins Proteins 0.000 description 8
- 108010085346 steroid delta-isomerase Proteins 0.000 description 8
- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 description 7
- 239000007990 PIPES buffer Substances 0.000 description 7
- 238000013016 damping Methods 0.000 description 7
- 239000012530 fluid Substances 0.000 description 7
- 238000005199 ultracentrifugation Methods 0.000 description 7
- AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 description 6
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 6
- 230000005484 gravity Effects 0.000 description 6
- IRQRBVOQGUPTLG-UHFFFAOYSA-M sodium;3-(n-ethyl-3-methylanilino)-2-hydroxypropane-1-sulfonate Chemical compound [Na+].[O-]S(=O)(=O)CC(O)CN(CC)C1=CC=CC(C)=C1 IRQRBVOQGUPTLG-UHFFFAOYSA-M 0.000 description 6
- JHIVVAPYMSGYDF-UHFFFAOYSA-N cyclohexanone Chemical compound O=C1CCCCC1 JHIVVAPYMSGYDF-UHFFFAOYSA-N 0.000 description 5
- 239000000852 hydrogen donor Substances 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 229940098773 bovine serum albumin Drugs 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 229920001983 poloxamer Polymers 0.000 description 4
- ZDSCFBCGDDCJFZ-UHFFFAOYSA-N 2-(methylamino)ethanesulfonic acid;sodium Chemical compound [Na].CNCCS(O)(=O)=O ZDSCFBCGDDCJFZ-UHFFFAOYSA-N 0.000 description 3
- 201000001320 Atherosclerosis Diseases 0.000 description 3
- 102000004157 Hydrolases Human genes 0.000 description 3
- 108090000604 Hydrolases Proteins 0.000 description 3
- RVGRUAULSDPKGF-UHFFFAOYSA-N Poloxamer Chemical compound C1CO1.CC1CO1 RVGRUAULSDPKGF-UHFFFAOYSA-N 0.000 description 3
- 125000002252 acyl group Chemical group 0.000 description 3
- 150000001768 cations Chemical class 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 239000007795 chemical reaction product Substances 0.000 description 3
- DDXLVDQZPFLQMZ-UHFFFAOYSA-M dodecyl(trimethyl)azanium;chloride Chemical group [Cl-].CCCCCCCCCCCC[N+](C)(C)C DDXLVDQZPFLQMZ-UHFFFAOYSA-M 0.000 description 3
- 230000000505 pernicious effect Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 2
- PAYRUJLWNCNPSJ-UHFFFAOYSA-N Aniline Chemical compound NC1=CC=CC=C1 PAYRUJLWNCNPSJ-UHFFFAOYSA-N 0.000 description 2
- 101000847066 Homo sapiens Translin-associated protein X Proteins 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 108010028554 LDL Cholesterol Proteins 0.000 description 2
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
- 102100032834 Translin-associated protein X Human genes 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 150000001841 cholesterols Chemical class 0.000 description 2
- 239000002872 contrast media Substances 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 230000003111 delayed effect Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 150000002500 ions Chemical class 0.000 description 2
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 description 2
- INHCSSUBVCNVSK-UHFFFAOYSA-L lithium sulfate Chemical compound [Li+].[Li+].[O-]S([O-])(=O)=O INHCSSUBVCNVSK-UHFFFAOYSA-L 0.000 description 2
- 229920002114 octoxynol-9 Polymers 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 229920000259 polyoxyethylene lauryl ether Polymers 0.000 description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 description 2
- 235000011152 sodium sulphate Nutrition 0.000 description 2
- DYPGTLUYQZIULN-UHFFFAOYSA-N 3-(3-methoxy-5-methylanilino)propane-1-sulfonic acid Chemical compound COC1=CC(C)=CC(NCCCS(O)(=O)=O)=C1 DYPGTLUYQZIULN-UHFFFAOYSA-N 0.000 description 1
- ZSQAETBIVMCCPM-UHFFFAOYSA-N C(CCCCCCCCCCC)C(C[Na])(N)N Chemical compound C(CCCCCCCCCCC)C(C[Na])(N)N ZSQAETBIVMCCPM-UHFFFAOYSA-N 0.000 description 1
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 208000006558 Dental Calculus Diseases 0.000 description 1
- 102100031780 Endonuclease Human genes 0.000 description 1
- 108010042407 Endonucleases Proteins 0.000 description 1
- 241001191009 Gymnomyza Species 0.000 description 1
- 102100031415 Hepatic triacylglycerol lipase Human genes 0.000 description 1
- 102100037611 Lysophospholipase Human genes 0.000 description 1
- 108020002496 Lysophospholipase Proteins 0.000 description 1
- 229910021380 Manganese Chloride Inorganic materials 0.000 description 1
- GLFNIEUTAYBVOC-UHFFFAOYSA-L Manganese chloride Chemical compound Cl[Mn]Cl GLFNIEUTAYBVOC-UHFFFAOYSA-L 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 102000011420 Phospholipase D Human genes 0.000 description 1
- 108090000553 Phospholipase D Proteins 0.000 description 1
- 108010058864 Phospholipases A2 Proteins 0.000 description 1
- 229920002025 Pluronic® F 88 Polymers 0.000 description 1
- 229920002057 Pluronic® P 103 Polymers 0.000 description 1
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 102000014384 Type C Phospholipases Human genes 0.000 description 1
- 108010079194 Type C Phospholipases Proteins 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 125000001931 aliphatic group Chemical group 0.000 description 1
- 235000019270 ammonium chloride Nutrition 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000003593 chromogenic compound Substances 0.000 description 1
- 239000005515 coenzyme Substances 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- USIUVYZYUHIAEV-UHFFFAOYSA-N diphenyl ether Chemical compound C=1C=CC=CC=1OC1=CC=CC=C1 USIUVYZYUHIAEV-UHFFFAOYSA-N 0.000 description 1
- JRBPAEWTRLWTQC-UHFFFAOYSA-N dodecylamine Chemical compound CCCCCCCCCCCCN JRBPAEWTRLWTQC-UHFFFAOYSA-N 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
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- 239000011544 gradient gel Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- UEGPKNKPLBYCNK-UHFFFAOYSA-L magnesium acetate Chemical compound [Mg+2].CC([O-])=O.CC([O-])=O UEGPKNKPLBYCNK-UHFFFAOYSA-L 0.000 description 1
- 239000011654 magnesium acetate Substances 0.000 description 1
- 235000011285 magnesium acetate Nutrition 0.000 description 1
- 229940069446 magnesium acetate Drugs 0.000 description 1
- 229910001629 magnesium chloride Inorganic materials 0.000 description 1
- 239000011565 manganese chloride Substances 0.000 description 1
- 235000002867 manganese chloride Nutrition 0.000 description 1
- DXYYSGDWQCSKKO-UHFFFAOYSA-N methylbenzothiazole Natural products C1=CC=C2SC(C)=NC2=C1 DXYYSGDWQCSKKO-UHFFFAOYSA-N 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 150000003016 phosphoric acids Chemical class 0.000 description 1
- 229920000447 polyanionic polymer Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 230000036632 reaction speed Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000013214 routine measurement Methods 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- SVLRFMQGKVFRTB-UHFFFAOYSA-M sodium;3-(3,5-dimethoxyanilino)-2-hydroxypropane-1-sulfonate Chemical compound [Na+].COC1=CC(NCC(O)CS([O-])(=O)=O)=CC(OC)=C1 SVLRFMQGKVFRTB-UHFFFAOYSA-M 0.000 description 1
- HLXGRHNZZSMNRX-UHFFFAOYSA-M sodium;3-(n-ethyl-3,5-dimethylanilino)-2-hydroxypropane-1-sulfonate Chemical compound [Na+].[O-]S(=O)(=O)CC(O)CN(CC)C1=CC(C)=CC(C)=C1 HLXGRHNZZSMNRX-UHFFFAOYSA-M 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 229960004418 trolamine Drugs 0.000 description 1
- 238000007817 turbidimetric assay Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/92—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/10—Composition for standardization, calibration, simulation, stabilization, preparation or preservation; processes of use in preparation for chemical testing
- Y10T436/104165—Lipid, cholesterol, or triglyceride standard or control
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
- Y10T436/25125—Digestion or removing interfering materials
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Abstract
Description
PIPES缓冲液(pH 7.0) | 50mmol/L |
胆固醇酯酶 | 0.6U/mL |
胆固醇氧化酶 | 0.5U/mL |
聚氧化乙烯-联苯乙烯苯基醚EMULGEN A-90 | 0.3g/L |
牛血清白蛋白 | 0.5% |
TOOS | 2.0mmol/L |
4-氨基安替比林 | 4.0mmol/L |
过氧化物酶 | 12.0unit/mL |
PIPES缓冲液(pH 7.0) | 50mmol/L |
胆固醇酯酶[Asahi Kasei公司] | 0.6U/mL |
胆固醇氧化酶[Toyobo] | 0.5U/mL |
过氧化氢酶 | 600U/mL |
聚氧化乙烯苯甲基苯基醚 | 0-1.2g/L |
牛血清白蛋白 | 1.0% |
TOOS | 2.0mmol/L |
PIPES缓冲液(pH 7.0) | 50mmol/L |
聚氧化乙烯烷基苯基醚 | 10g/L |
4-氨基安替比林 | 4.0mmol/L |
过氧化物酶 | 4.0unit/mL |
叠氮化钠 | 0.05% |
PIPES缓冲液(pH 7.0) | 50mmol/L |
胆固醇酯酶[Asahi Kasei公司] | 1.2U/mL |
胆固醇氧化酶[Toyobo] | 0.5U/mL |
过氧化氢酶 | 600U/mL |
各种表面活性剂 | 0.3g/L or 0.6g/L |
牛血清白蛋白 | 1.0% |
TOOS | 2.0mmol/L |
PIPES缓冲液(pH 7.0) | 50mmol/L |
聚氧化乙烯烷基苯基醚 | 10g/L |
4-氨基安替比林 | 4.0mmol/L |
过氧化物酶 | 4.0unit/mL |
叠氮化钠 | 0.05% |
在第一步中表面活性剂的浓度(g/L) | 小而密LDL(mAbs) | 大LDL(mAbs) | sdLDL/LLDL% | 组合物 | |
LDL-EXN | - | 71.5 | 71.6 | 100% | |
EMULGEN120 | 0.3 | 58.8 | 41.2 | 143% | 聚氧化乙烯月桂基醚 |
EMULGEN920 | 0.3 | 37.8 | 18.8 | 201% | 聚氧化乙烯壬基苯基醚 |
EMULGENB66 | 0.3 | 34.9 | 14.2 | 246% | 聚氧化乙烯三苄基苯基醚 |
EMULGENA90 | 0.3 | 57.8 | 31.5 | 183% | 聚氧化乙烯联苯乙烯苯基醚 |
NONIONHS215 | 0.3 | 33.8 | 17.6 | 192% | 聚氧化乙烯辛基苯基醚 |
NONION | 0.6 | 55.7 | 32.6 | 171% | 聚氧化乙烯辛基苯基醚 |
HS-220 | |||||
NONIONK230 | 0.3 | 56.9 | 41.7 | 136% | 聚氧化乙烯月桂基醚 |
NONIONNS220 | 0.3 | 34.5 | 18.0 | 192% | 聚氧化乙烯壬基苯基醚 |
NONIONNS230 | 0.6 | 62.0 | 40.3 | 154% | 聚氧化乙烯壬基苯基醚 |
NYMEENL-207 | 0.3 | 62.9 | 51.6 | 122% | 聚氧化乙烯十二烷基胺 |
NYMEENF215 | 0.3 | 61.1 | 47.0 | 130% | 聚氧化乙烯烷基胺 |
ADEKA TOLLB-1520 | 0.3 | 78.5 | 65.9 | 119% | 烷氧基化月桂醇 |
EMAL 20CM | 0.6 | 27.5 | 18.1 | 152% | 聚氧化乙烯烷基醚硫酸钠 |
EMAL 20T | 0.6 | 36.0 | 24.4 | 148% | 三乙醇胺聚氧化乙烯烷基醚硫酸盐 |
EMAL E27C | 0.6 | 26.8 | 19.0 | 141% | 聚氧化乙烯月桂基醚硫酸钠 |
LEVENOLWX | 0.3 | 19.1 | 12.2 | 157% | 聚氧化乙烯烷基醚硫酸钠 |
SUNAMIDEC-3 | 0.6 | 29.6 | 20.3 | 146% | 酰胺醚硫酸盐 |
SUNAMIDECF-10 | 0.6 | 36.5 | 24.2 | 151% | 酰胺醚硫酸盐 |
SUNAMIDECF-3 | 0.3 | 18.5 | 12.5 | 148% | 酰胺醚硫酸盐 |
DIAPON K | 0.6 | 45.2 | 34.3 | 132% | 酰基甲基牛磺酸钠 |
DIAPON F | 0.3 | 19.3 | 15.2 | 127% | 酰基甲基牛磺酸钠 |
DIAPONK-SF | 0.3 | 14.7 | 11.0 | 134% | 酰基甲基牛磺酸钠 |
PERSOFT EF | 0.6 | 29.3 | 20.7 | 142% | 烷基醚硫酸盐 |
PERSOFTEFT | 0.6 | 39.1 | 28.9 | 135% | 烷基醚硫酸盐 |
PERSOFT EL | 0.3 | 25.6 | 17.4 | 147% | 烷基醚硫酸盐 |
PERSOFT EP | 0.6 | 31.0 | 21.4 | 145% | 烷基醚硫酸盐 |
PERSOFT EK | 0.3 | 21.9 | 15.4 | 142% | 烷基醚硫酸盐 |
PERSOFT SL | 0.6 | 43.2 | 32.4 | 133% | 烷基硫酸盐 |
POLYSTAROMP | 0.3 | 41.6 | 31.2 | 133% | 高分子型阴离子 |
ADEKA COLPS-440E | 0.3 | 56.2 | 27.1 | 207% | 脂族磷酸酯 |
TRAX K-40 | 0.3 | 18.6 | 13.6 | 137% | 特定阴离子 |
QUARTAMIN24P | 0.6 | 60.8 | 43.8 | 139% | 月桂基三甲基氯化铵 |
ADEKAMINE4MAC-30 | 0.6 | 57.2 | 44.4 | 129% | 单线性烷基型 |
AMPHITOL24B | 0.3 | 65.1 | 51.1 | 127% | 月桂基甜菜碱 |
NISSANANONLG | 0.6 | 58.9 | 38.7 | 152% | 月桂基二氨基乙基甘氨酸钠 |
NISSANANON BDF-R | 0.3 | 23.0 | 16.7 | 138% | 酰胺甜菜碱型 |
NISSANANONBF | 0.3 | 62.3 | 48.6 | 128% | 二甲基烷基甜菜碱 |
NISSANANONBL | 0.3 | 56.0 | 45.2 | 124% | 二甲基烷基甜菜碱 |
NISSANANON BL-SF | 0.3 | 64.6 | 54.2 | 119% | 二甲基烷基甜菜碱 |
NISSANANONGLM-R-LV | 0.6 | 25.0 | 14.7 | 170% | 咪唑啉型 |
PIPES缓冲液(pH 7.0) | 50mmol/L |
胆固醇酯酶[Asahi Kasei公司] | 0.6-3.0U/mL |
胆固醇氧化酶[Toyobo] | 0.5U/mL |
过氧化氢酶 | 600U/mL |
聚氧化乙烯-联苯乙烯苯基醚[Kao公司] | 0.03% |
牛血清白蛋白 | 0.5% |
TOOS | 2.0mmol/L |
PIPES缓冲液(pH 7.0) | 50mmol/L |
聚氧化乙烯-聚氧化丙烯共聚物Pluronic 17R-4[ADEKA公司] | 0.3% |
4-氨基安替比林 | 4.0mmol/L |
过氧化物酶 | 4.0unit/mL |
叠氮化钠 | 0.05% |
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ES2397942T3 (es) * | 2006-05-01 | 2013-03-12 | Denka Seiken Co., Ltd. | Método para la detección de hiperlipidemia combinada familiar |
EP2208794B1 (en) * | 2007-10-10 | 2016-04-27 | DENKA SEIKEN Co., Ltd. | Method and kit for quantification of small, dense ldl cholesterol |
WO2011136316A1 (ja) * | 2010-04-30 | 2011-11-03 | 協和メデックス株式会社 | 低密度リポ蛋白中のコレステロールの測定方法、測定用試薬及び測定用キット |
EP2597468B1 (en) * | 2010-07-23 | 2017-03-15 | Denka Seiken Co., Ltd. | Method for quantifying the amount of cholesterol in high-density lipoprotein 3 |
WO2012118017A1 (ja) * | 2011-02-28 | 2012-09-07 | デンカ生研株式会社 | 高密度リポタンパク質2中のコレステロールの定量方法およびそのための試薬キット |
JP6054051B2 (ja) * | 2012-04-11 | 2016-12-27 | デンカ生研株式会社 | 高密度リポ蛋白(hdl)中のコレステロール(−c)の亜分画定量方法 |
CA3194562A1 (en) * | 2014-07-29 | 2016-02-04 | Shenzhen Hightide Biopharmaceutical, Ltd. | Berberine-ursodeoxycholic salt, method of preparation and application therof |
CN105652021B (zh) * | 2016-03-11 | 2018-01-02 | 上海练佰生物技术中心 | 一种测定小而密脂蛋白的试剂、方法和试剂盒 |
CN107446989B (zh) * | 2017-06-23 | 2020-07-14 | 美康生物科技股份有限公司 | 测定样本中的小而密低密度脂蛋白胆固醇的方法及试剂 |
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JP7311248B2 (ja) * | 2018-08-08 | 2023-07-19 | デンカ株式会社 | リポ蛋白コレステロールの定量方法及び定量キット |
JP6869300B2 (ja) * | 2019-09-10 | 2021-05-12 | デンカ株式会社 | small,dense LDLコレステロールの定量方法およびキット |
JP6703175B1 (ja) * | 2019-09-10 | 2020-06-03 | デンカ生研株式会社 | キットおよび方法 |
JP7427522B2 (ja) * | 2019-09-10 | 2024-02-05 | デンカ株式会社 | キットおよび方法 |
CN110791549B (zh) * | 2019-12-03 | 2023-09-26 | 宁波普瑞柏生物技术股份有限公司 | 定量测定小而密低密度脂蛋白胆固醇的方法和试剂盒 |
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