CN101613763A - 诊断沙眼衣原体、***及解脲支原体感染的荧光pcr方法 - Google Patents
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Abstract
Description
基因型 | 基因库编号(GI No.) | 靶序列位置 | 扩增产物长度(bp) |
CT | FM865442 | 2160-2649 | 480 |
NG | AM921674 | 60-540 | 481 |
UU | AF085729 | 241-720 | 480 |
原料名称 | 终浓度 |
PCR Buffer | (0.8-2)× |
MgCl2 | 2-5mM |
dNTP | 0.1-0.5mM |
引物 | 各0.01-0.50μM |
探针 | 各0.01-0.50μM |
UNG | 0.05-1U |
Taq酶 | 0.5-3U |
H2O | 适量 |
DNA模板 | 2μl |
终体积 | 40μl |
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102409098A (zh) * | 2011-11-25 | 2012-04-11 | 泰普生物科学(中国)有限公司 | 一种***pcr检测试剂盒及其检测方法 |
CN103184272A (zh) * | 2011-12-27 | 2013-07-03 | 上海复星医学科技发展有限公司 | 沙眼衣原体、***、细小脲原体和***的检测试剂盒 |
CN103409508A (zh) * | 2013-07-02 | 2013-11-27 | 江苏硕世生物科技有限公司 | 一种***/解脲支原体/沙眼衣原体三重核酸检测试剂盒 |
CN103484534A (zh) * | 2013-06-19 | 2014-01-01 | 兰州百源基因技术有限公司 | 一组用于淋病奈瑟氏菌实时荧光pcr检测的引物和探针及其应用 |
CN103555842A (zh) * | 2013-11-05 | 2014-02-05 | 重庆出入境检验检疫局检验检疫技术中心 | 动物衣原体Taqman-MGB探针多重实时荧光定量PCR检测用引物、试剂盒和方法 |
CN105349660A (zh) * | 2015-11-27 | 2016-02-24 | 湖南圣湘生物科技有限公司 | 一种沙眼衣原体/***核酸检测试剂盒 |
CN105349661A (zh) * | 2015-11-27 | 2016-02-24 | 湖南圣湘生物科技有限公司 | 一种沙眼衣原体/***核酸检测试剂盒 |
CN107937580A (zh) * | 2017-12-26 | 2018-04-20 | 湖南圣湘生物科技有限公司 | 泌尿生殖道微生物检测用的引物和探针、试剂盒及试剂盒的使用方法 |
CN110373485A (zh) * | 2019-07-30 | 2019-10-25 | 中山大学达安基因股份有限公司 | 一种***、沙眼衣原体和***三联检试剂盒 |
WO2022228187A1 (zh) * | 2021-04-26 | 2022-11-03 | 西南医科大学附属医院 | 一种解脲支原体、沙眼衣原体和***检测的试剂盒及方法 |
-
2009
- 2009-07-30 CN CN 200910164977 patent/CN101613763B/zh active Active
Non-Patent Citations (5)
Title |
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张德 等: "荧光定量PCR对NG、CT和UU三种性病的检测分析", 《中国误诊学杂志》 * |
张德 等: "荧光定量PCR检测N G、CT和U U三种性病混合", 《中国民康医学》 * |
李成志 等: "女性生殖道沙眼衣原体感染", 《使用妇产科杂志》 * |
程钢 等: "沙眼衣原体荧光PCR试剂盒的研制及临床考核", 《中山医科大学学报》 * |
黄敏 等: "荧光定量PCR检测常见3种性病病原体结果分析", 《重庆医学》 * |
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102409098A (zh) * | 2011-11-25 | 2012-04-11 | 泰普生物科学(中国)有限公司 | 一种***pcr检测试剂盒及其检测方法 |
CN103184272A (zh) * | 2011-12-27 | 2013-07-03 | 上海复星医学科技发展有限公司 | 沙眼衣原体、***、细小脲原体和***的检测试剂盒 |
CN103484534A (zh) * | 2013-06-19 | 2014-01-01 | 兰州百源基因技术有限公司 | 一组用于淋病奈瑟氏菌实时荧光pcr检测的引物和探针及其应用 |
CN103409508A (zh) * | 2013-07-02 | 2013-11-27 | 江苏硕世生物科技有限公司 | 一种***/解脲支原体/沙眼衣原体三重核酸检测试剂盒 |
CN103409508B (zh) * | 2013-07-02 | 2015-05-13 | 江苏硕世生物科技有限公司 | 一种***/解脲支原体/沙眼衣原体三重核酸检测试剂盒 |
CN103555842A (zh) * | 2013-11-05 | 2014-02-05 | 重庆出入境检验检疫局检验检疫技术中心 | 动物衣原体Taqman-MGB探针多重实时荧光定量PCR检测用引物、试剂盒和方法 |
CN103555842B (zh) * | 2013-11-05 | 2015-05-27 | 重庆出入境检验检疫局检验检疫技术中心 | 动物衣原体Taqman-MGB探针多重实时荧光定量PCR检测用引物、试剂盒和方法 |
CN105349660A (zh) * | 2015-11-27 | 2016-02-24 | 湖南圣湘生物科技有限公司 | 一种沙眼衣原体/***核酸检测试剂盒 |
CN105349661A (zh) * | 2015-11-27 | 2016-02-24 | 湖南圣湘生物科技有限公司 | 一种沙眼衣原体/***核酸检测试剂盒 |
CN107937580A (zh) * | 2017-12-26 | 2018-04-20 | 湖南圣湘生物科技有限公司 | 泌尿生殖道微生物检测用的引物和探针、试剂盒及试剂盒的使用方法 |
CN110373485A (zh) * | 2019-07-30 | 2019-10-25 | 中山大学达安基因股份有限公司 | 一种***、沙眼衣原体和***三联检试剂盒 |
WO2022228187A1 (zh) * | 2021-04-26 | 2022-11-03 | 西南医科大学附属医院 | 一种解脲支原体、沙眼衣原体和***检测的试剂盒及方法 |
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