CN101589721A - A kind of control medicament of the viroses of plant - Google Patents
A kind of control medicament of the viroses of plant Download PDFInfo
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Abstract
The present invention relates to a kind of viroses of plant control medicament.Viroses of plant control medicament of the present invention is made up of the active principle of following weight portion: Moroxydine Hydrochloride 4.0～97.0; Amino-oligosaccharide 0.05～20.0.In addition, described control medicament can also comprise the solubility filler of following weight portion: 2.0～96.0.Control medicament of the present invention utilizes the synergy of antiviral agent Moroxydine Hydrochloride and resistance inductors amino-oligosaccharide, the generation and the development of prevention and control crop virosis, have prevention and therapeutic action concurrently, premorbid or morbidity back use separately can reach remarkable prevention effect, and the medication optimum period is long, dosage is little, cost is low.
Description
Technical field
The present invention relates to a kind of agricultural chemicals, particularly relate to a kind of viroses of plant control medicament.
Background technology
The viroses of plant are widely distributed, and are of a great variety, and the route of transmission is wide, and harm is serious.Have now found that 716 kinds, can be divided into ssRNA, dsRNA, ssDNA, dsDNA, viroids, pseudovirus, prion, satellite virus etc., nearly all cultivated plant all suffers a kind of harm of virus at least, and in 209 kinds of vegetables on the books (not containing kind), just infecting has 195 kinds of viruses.The viroses of plant mainly by biological amboceptor such as aphid, cicada, nematode, fungi, mite etc. carry out semi-persistent, persistence is propagated, also can pass through seed, pollen, vegetative propagule, people, animal and agricultural tillage measure propagates, by the nutrient contention, disturb the plant physiology metabolism, produce symptoms such as floral leaf, necrosis, deformity, directly influence the yield and quality of cultivated plant, the lighter's underproduction 10-20%, more than weight person's underproduction 30-50%, agricultural production is caused serious harm.
The method of preventing and treating of the viroses of plant mainly contains: 1) breeding resistant variety, seed or trophosome detoxification, utilize attenuated strain system and satellite RNA; 2) kill the biological virus mediator that passes; 3) regulate date of seeding, improve cropping system etc.; 4) chemical agent control.Still not having at present desirable disease-resistant variety, is that the comprehensive preventive health measures at center is a kind of preventive measure to promote disease-resistant variety, and lacking the effectively preventing medicament is the principal element that viroses of plant wide-scale distribution, large tracts of land spread.
Widely used aborning at present viral diseases agricultural chemicals, prescription form single agent of mostly being Moroxydine Hydrochloride, ribavirin, amino-oligosaccharide or with the mixture of zinc sulphate or copper sulphate or the copper acetate or the basic element of cell division etc., it is better all to use preventive effect at premorbid, and the result of treatment of using after morbidity is poor.
For this reason, be necessary to provide all significant viroses of plant control of a kind of prevention and result of treatment medicament.
Summary of the invention
The purpose of this invention is to provide a kind of viroses of plant control medicament.
Viroses of plant control medicament of the present invention is made up of the active principle of following weight portion: Moroxydine Hydrochloride: 4.0～97.0; Amino-oligosaccharide: 0.05～20.0.
Preferably, the weight portion of amino-oligosaccharide is 0.1～15.0.
Above-mentioned viroses of plant control medicament comprises that also weight portion is: 2.0～96.0 solubility filler, described solubility filler is mineral salt or the organic matter that contains plant nutrient ingredient, as contain the soluble inorganic salt of elements such as calcium, manganese, copper, the urea that contains nitrogen contains the humate of humus etc.
Wherein, Moroxydine Hydrochloride (Moroxydine hydrochloride) is a kind of antiviral agent, it can directly enter the plant living body cell, the protein coat and the interior nucleic acid of shell that duplicate, suppress or destroy the formation virion of prevention or break virus, thus reduce viral diffusivity.
Amino-oligosaccharide (oligosaccharins) is a kind of plant resistance to environment stress derivant, chemical name β-1,4-oligomerization-gucosamine.But the disease resistance of amino-oligosaccharide inducing plant produces immunity and killing action to multiple fungi, bacterium and virus, and diseases such as wheat mosaic, cotton verticillium wilt, rice blast, tomato late blight are had good preventive and therapeutic effect; Simultaneously, amino-oligosaccharide has to a certain degree direct repression to the various plants pathogen; And amino-oligosaccharide itself contains abundant C, N, can be utilized by microbial decomposition and as the nutrient of plant growing.
The inventor finds, after above-mentioned two kinds of active principle Moroxydine Hydrochlorides and amino-oligosaccharide are mixed, also has certain synergy, can increase the effect of control crop virosis, after the medication of morbidity strain just 3-5 days, growing point can be good at normal young leaves, and the deformed vanes that virus disease causes is had the flattening effect.
The viroses of plant of the present invention control medicament can be mixed with various types of preparations according to technology well known in the art, for example pulvis, aqua etc., preferred solvable pulvis
The present invention prevents and treats medicament and generally adopts foliage applying, and its preparation amount of application is generally 25-50g/ mu, and the active ingredient spraying concentration is generally 50-750ppm.Those skilled in the art can adjust consumption as required.
Viroses of plant control medicament of the present invention is mixed by antiviral agent Moroxydine Hydrochloride and plant resistance to environment stress derivant amino-oligosaccharide and forms, and utilizes the synergy of antiviral activity and induction of resistance, the generation and the development of control crop virosis.The present invention prevents and treats crop virosis and has prevention and therapeutic action concurrently, and premorbid or morbidity back use separately can reach remarkable prevention effect, and the medication optimum period is long, dosage is little, cost is low.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1
The formulation components of present embodiment is set to the test of two-factor four horizontal quadratures, Moroxydine Hydrochloride mass percent in the described viroses of plant control medicament is respectively 5%, 30%, 60%, 95%, the amino-oligosaccharide mass percent is respectively 0.2%, 1.0%, 4.0%, 12%, and all the other are filler: potassium chloride.
Select the single agent of above-mentioned each component granularity between 0.01～0.99mm, said components is evenly mixed in proportion, can be processed into solvable pulvis.
1. test material and method
Test medicine is prepared by patent inventor of the present invention, test is handled and is set to: 15 of the different component preparation combinations of Moroxydine Hydrochloride and amino-oligosaccharide, contrast medicament 2% amino-oligosaccharide aqua (commercially available product), 20% moroxydine second copper wetting powder (commercially available product), clear water contrast totally 18 processing.Experimental group is as shown in table 1.
Table 1 test is handled and is provided with
| Sequence number | Test is handled |
| 1 | 5.0% Moroxydine Hydrochloride+0.2% amino-oligosaccharide+94.8% potassium chloride |
| 2 | 5.0% Moroxydine Hydrochloride+1.0% amino-oligosaccharide+96.0% potassium chloride |
| 3 | 5.0% Moroxydine Hydrochloride+4.0% amino-oligosaccharide+91.0% potassium chloride |
| 4 | 5.0% Moroxydine Hydrochloride+12.0% amino-oligosaccharide+83.0% potassium chloride |
| 5 | 30.0% Moroxydine Hydrochloride+0.2% amino-oligosaccharide+69.8% potassium chloride |
| 6 | 30.0% Moroxydine Hydrochloride+1.0% amino-oligosaccharide+69.0% potassium chloride |
| 7 | 30.0% Moroxydine Hydrochloride+4.0% amino-oligosaccharide+66.0% potassium chloride |
| 8 | 30.0% Moroxydine Hydrochloride+12.0% amino-oligosaccharide+58.0% potassium chloride |
| 9 | 60.0% Moroxydine Hydrochloride+0.2% amino-oligosaccharide+39.8% potassium chloride |
| 10 | 60.0% Moroxydine Hydrochloride+1.0% amino-oligosaccharide+39.0% potassium chloride |
| 11 | 60.0% Moroxydine Hydrochloride+4.0% amino-oligosaccharide+36.0% potassium chloride |
| 12 | 60.0% Moroxydine Hydrochloride+12.0% amino-oligosaccharide+28.0% potassium chloride |
| 13 | 95.0% Moroxydine Hydrochloride+0.2% amino-oligosaccharide+4.8% potassium chloride |
| 14 | 95.0% Moroxydine Hydrochloride+1.0% amino-oligosaccharide+4.0% potassium chloride |
| 15 | 95.0% Moroxydine Hydrochloride+4.0% amino-oligosaccharide+1.0% potassium chloride |
| 16 | 2% amino-oligosaccharide aqua |
| 17 | 20% moroxydine second copper wetting powder |
| 18 | The clear water contrast |
Test is located at four Fang Xiang agrotechnical stations, Hua County, Henan Province, the subjects tomato virus disease, and kind is cooperation 901, seed is bought by market.To grow seedlings in seedling-cultivating tray after the tomato seeds vernalization, be transplanted to diameter during 3～4 true leaves and be in 32 centimetres the flowerpot, every basin is planted 4 strains.Each handles plantation 8 basins, and per 2 basins are one group, repeat 4 times.After seedling is returned in transplanting, gather the virus disease blade from the field, extract viral juice, use juice rubbing manipulation virus inoculation disease then with the phosphate buffer of 0.1M.Inoculate after 3 days, each processed group atomizer for medicine even spraying sprayed once at interval in 7 days, sprayed altogether 2 times.After the dispenser second time 7 days, each incidence of handling of investigation.State of an illness grade scale is as follows:
0 grade: complete stool is anosis;
1 grade: bright arteries and veins of blade and slight flower leaf paresthesia;
3 grades: blade has tangible flower leaf paresthesia;
5 grades: blade has heavy flower leaf paresthesia, and Zou contracts, and plant is slightly downgraded (below 1/3);
7 grades: the heavy floral leaf of blade, narrow down, plant is obviously downgraded (1/3-2/3), and petiole base has slight downright bad streak;
9 grades: leaf malformation, plant are seriously downgraded (more than 2/3), and base portion or growing point necrosis or complete stool are withered.
(I and II) calculates preventive effect as follows:
2. test results and analysis
Result of the test is as shown in table 2:
Table 2 test medicine control tomato virus disease result of the test
The result shows, in the scope that formulation components is provided with, the Moroxydine Hydrochloride mass percent is respectively 5%, 30%, 60%, 95%, the amino-oligosaccharide mass percent is respectively 0.2%, 1.0%, 4.0%, 12%, with mineral salt potassium chloride is the solvable pulvis of the different component of filler preparation, the effect of control tomato virus disease is 67.9%～87.8%, and contrast medicament 2% amino-oligosaccharide aqua, 20% moroxydine second copper wetting powder are respectively 56.5%, 58.7%.Through significance of difference analysis, the preventive effect of the different component preparation of Moroxydine Hydrochloride and amino-oligosaccharide all extremely significantly is better than 2% amino-oligosaccharide aqua, 20% moroxydine second copper wetting powder.
The embodiment 230% moroxydine+solvable pulvis of 1% amino-oligosaccharide
Formulation components: Moroxydine Hydrochloride 30.0%; Amino-oligosaccharide 1.0%; Calcium chloride filler 69.0%.
Compound method: it is between 0.01～0.99mm that above-mentioned each component is crushed to granularity, and said components is evenly mixed according to the above ratio, is processed into solvable pulvis with comminution by gas stream.
1. test material and method
Experimental field be located at Fan County Chen Zhuan, fertilizer and water condition is good, and irrigation and drainage are convenient, and soil fertility is even.Rice varieties is No. 6, Henan round-grained rice, the preceding crop wheat.Rice transplanting on June 18 in 2008 waits the line-spacing plantation, 0.3 meter of line-spacing, 0.12 meter of cave distance, 1.80 ten thousand clumps/mu of density.Other field management is normal in the process of the test.
Test medicine: " 30% moroxydine+1% amino-oligosaccharide " solvable pulvis, the inventor provides; Contrast medicament: commercially available product 20% moroxydine second copper wetting powder, commercially available product.
Test is handled and is set to 600 times of liquid of " 30% moroxydine+1% amino-oligosaccharide " solvable pulvis, 900 times of liquid, 1200 times of liquid, 20% 400 times of liquid of moroxydine second copper wetting powder and 5 processing of clear water contrast, repeat for 4 times, totally 20 sub-districts, district's group is arranged at random.Sub-district area 30m
2, 10 row districts, the long 10m of row.In the first period of disease of stripe disease: beginning on July 2 medication for the first time, spray 30 kilograms of amount of liquid medicine every mu time, at interval 7 days once, spray altogether 2 times.Every sub-district is by selected 5 points of diagonal method, 5 clumps of every some marks.Investigation state of an illness radix before the dispenser on July 2, July 17, promptly after the dispenser for the second time 7 days, the stem tiller incidence of investigation mark clump.State of an illness grade scale is as follows:
0 grade: anosis, or morbidity stem tiller grows normal new leave;
1 grade: the lobus cardiacus base portion a small amount of chlorisis macula lutea occurs along vein, does not curl;
3 grades: the yellowish green alternate striped parallel with vein appears in new leave, slight curling;
5 grades: a large amount of chlorosis stripeds parallel with vein, leaf rolling, thin and delicate appear in new leave;
7 grades: plant is downgraded, and the yellow-white striped appears in blade to be rolled, and new leave distortion is sagging, can not normally open a business;
9 grades: seriously dwarfing of plant, chlorosis or death.
Calculate preventive effect by above-mentioned formula (I) and following formula (III):
2. test results and analysis
2.1 result of the test
Result of the test shows, the average preventive effect of 600 times of liquid of " 30% moroxydine+1% amino-oligosaccharide " solvable pulvis, 900 times of liquid, 1200 times of liquid is respectively 80.6%, 77.7%, 71.9%, and the average preventive effect of contrast medicament 20% moroxydine second copper wetting powder is 53.8%.
Table 3 test medicine is prevented and treated stripe disease statistical effect table
By result of the test as can be seen, the effect that " 30% moroxydine+1% amino-oligosaccharide " solvable pulvis is prevented and treated stripe disease increases with the increase of concentration, through variance analysis, do not have significant difference between the preventive effect of 600 times of liquid of " 30% moroxydine+1% amino-oligosaccharide " solvable pulvis, 900 times of liquid, all significantly be better than the preventive effect of 1200 times of liquid; The preventive effect of 1200 times of liquid of " 30% moroxydine+1% amino-oligosaccharide " solvable pulvis significantly is better than contrasting the preventive effect of medicament.
2.2 to rice safety
Do not find in the test that " 30% moroxydine+1% amino-oligosaccharide " solvable pulvis produces other harmful effects such as poisoning to paddy rice, illustrate that " 30% moroxydine+1% amino-oligosaccharide " solvable pulvis is safe to paddy rice.Through experimental observation, " 30% moroxydine+1% amino-oligosaccharide " solvable pulvis is 10～15 days to the lasting period of stripe disease, and dispenser should be 7-10 days and be advisable interval." 30% moroxydine+1% amino-oligosaccharide " solvable pulvis not only has prevention effect to stripe disease, and has therapeutic action, and visible most of initial stage showed the dispenser of disease stem tiller after 5～7 days, grew normal newborn blade.
3. conclusion
As can be seen from the test results, " 30% moroxydine+1% amino-oligosaccharide " solvable pulvis is to rice safety, prevent and treat stripe disease and have prevention and therapeutic action concurrently, but premorbid and all medications of morbidity back, dispenser interval with 7～10 days or shorter time at interval as be advisable in 5～7 days, (preventive effect of active ingredient concentration 517～344ppm) is good with 600～900 times of liquid for spraying medicine concentration, consider that from cost-effective angle 1200 times of liquid (active ingredient concentration 258ppm) also can be applied and prevent on producing.
The embodiment 315% moroxydine+solvable pulvis of 1% amino-oligosaccharide
Formulation components: Moroxydine Hydrochloride 15.0%; Amino-oligosaccharide 1.0%; Calcium chloride filler 84.0%.
Compound method: select the component of granularity between 0.01～0.99mm for use, said components is evenly mixed in proportion, can be processed into solvable pulvis.
1. test material and method
Test is located at four rooms, Hua County, Henan Province township Wei Nan and exhales the village, the subjects tomato virus disease.Planting patterns was the transplanting of nursing young plants in hothouses, and seeding row spacing 40 * 50cm was transplanted in the back of growing seedlings May 15.Other field management is normal in the process of the test.
Test medicine: " 15% moroxydine+1% amino-oligosaccharide " solvable pulvis, patent applicant Guo Hong of the present invention just provides.Contrast medicament: 1. 2% amino-oligosaccharide aqua, commercially available product; 2. 20% moroxydine second copper wetting powder, commercially available product.
Test is handled and is set to 600 times of liquid of " 15% moroxydine+1% amino-oligosaccharide " solvable pulvis, 900 times of liquid, 1200 times of liquid, 900 times of liquid of contrast medicament 2% amino-oligosaccharide aqua, 20% 400 times of liquid of moroxydine second copper wetting powder and 6 processing of clear water contrast, each is handled 4 times and repeats, totally 24 sub-districts, district's group is arranged at random.The sub-district is 4 row districts, area 12m
2(2m * 6m), repeat 4 times.Dispenser 3 times, on June 7th, 2008, June 14 and dispenser on June 21.Tomato is in and comes into bloom the tomato virus disease initial phase during dispenser for the first time.Spraying volume is 450 kilograms/hectare, promptly 30 kilograms/mu.15 days (July 5), the tomato virus disease occurrence degree was respectively handled in investigation after the dispenser same day and last dispenser.
State of an illness stage division:
0 grade: asymptomatic;
1 grade: bright arteries and veins, light floral leaf;
3 grades: lobus cardiacus and middle leaf sheet floral leaf;
5 grades: lobus cardiacus and middle leaf sheet floral leaf, minority leaf malformation, shrinkage or plant are slightly downgraded;
7 grades: heavy floral leaf, most leaf malformations, shrinkage or plant are downgraded;
9 grades: heavy floral leaf, blade obviously deformity, line leaf, plant are seriously downgraded, even dead.
Drug effect is calculated according to above-mentioned formula (I and III).
2. test results and analysis
Result of the test sees Table 4:
Table 4 test medicine control tomato virus disease result of the test
600 times of liquid of preparation of the present invention " 15% moroxydine+1% amino-oligosaccharide " solvable pulvis, 900 times of liquid, 1200 times of liquid are respectively 71.7%, 68.6%, 67.7% to the control efficiency of tomato virus disease, all extremely significantly are better than contrasting the control efficiency 54.0%, 45.1% of 900 times of liquid of medicament 2% amino-oligosaccharide aqua, 400 times of liquid of 20% moroxydine second copper wetting powder.
Result of the test shows that the tomato virus disease initial phase begins medication, and after 3 medications 15 days, test medicine had certain control action to tomato virus disease.Statistical results show, preventive effect does not have marked difference between three processing of test medicine, all significantly is better than contrasting medicament.
The embodiment 430% moroxydine+solvable pulvis of 2% amino-oligosaccharide
Formulation components: Moroxydine Hydrochloride 30.0%; Amino-oligosaccharide 2.0%; Mineral salt manganese sulphate 68.0%.
Compound method: select the single agent of above-mentioned each component granularity between 0.01～0.99mm, said components is evenly mixed in proportion, can be processed into solvable pulvis.
1. test material and method
Test is located at Linying County Da Guo, the subjects tobacco virus, and tobacco bred is NC89, experimental field physical features is smooth, glutinous loam matter, pH value 6.9, water and fertilizer condition is good, and managerial skills are medium.0.5 meter of tobacco spacing in the rows, 1.0 meters of line-spacings, the ridging cultivation, the ridge is high 0.15 meter.Tobacco virus is based on tobacco mosaic virus and cucumber mosaic virus.
Test medicine: " 30% moroxydine+2% amino-oligosaccharide " solvable pulvis, patent applicant Guo Hong of the present invention just provides.Contrast medicament: 1. 2% amino-oligosaccharide aqua, commercially available product; 2. 20% moroxydine second copper wetting powder, commercially available product.
Test is handled and is set to 600 times of liquid of " 30% moroxydine+2% amino-oligosaccharide " solvable pulvis, 900 times of liquid, 1200 times of liquid, 900 times of liquid of contrast medicament 2% amino-oligosaccharide aqua, 20% 400 times of liquid of moroxydine second copper wetting powder and 6 processing of clear water contrast, each is handled 4 times and repeats, totally 24 sub-districts, district's group is arranged at random.Sub-district area 67m
2, repeat 4 times.
Test dispenser for the first time when June 15, tobacco virus was just sent out in 2008, dispenser in 7 days once (is respectively on June 22nd, 2008, on June 29th, 2008) at interval later on, and dispenser is 3 times altogether.Spray with WS-16 type (Shandong Wish Plant Protection Machinery Co., Ltd.) knapsack hand sprayer.30 kilograms of soups of mu.
5 samplings of sub-district diagonal, every continuous 5 strains of investigation, totally 25 strains, record diseased plant number and sick progression.7 days (July 5), the tobacco virus occurrence degree was respectively handled in investigation after the dispenser same day first time and last dispenser.Grade scale and relevant calculation formula are undertaken by " bactericidal agent for preventing and treating tobacco virus field control effectiveness test criterion ".
2. test results and analysis
Result of the test sees Table 5.The result shows, the tobacco virus initial phase begins medication, after 3 medications 7 days, 600 times of liquid of test medicine " 30% moroxydine+2% amino-oligosaccharide " solvable pulvis, 900 times of liquid, 1200 times of liquid are respectively 78.4%, 72.7%, 70.4% to the control efficiency of tobacco virus, and 900 times of liquid of contrast medicament 2% amino-oligosaccharide aqua, 400 times of liquid control efficiency of 20% moroxydine second copper wetting powder are respectively 60.1% and 56.9%.Statistical results show, each preventive effect of handling of test medicine all extremely significantly is better than contrasting medicament.
Table 5 control tobacco virus result of the test
The embodiment 545% moroxydine+solvable pulvis of 2% amino-oligosaccharide
Formulation components: Moroxydine Hydrochloride 45.0%; Amino-oligosaccharide 2.0%; Mineral salt ammonium sulfate 53.0%.
Compound method: select the single agent of above-mentioned each component granularity between 0.01～0.99mm, said components is evenly mixed in proportion, can be processed into solvable pulvis.
1. test material and method
Test is located at four rooms, Hua County, Henan Province, subjects watermelon virus disease.Experimental field water and fertilizer condition is better, No. 9, variety of watermelon Henan watermelon, and field planting on June 10 waits the line-spacing plantation, 1.85 meters of line-spacings, density 800 strain/mus, the watermelon virus disease takes place heavier throughout the year.Other field management are normal in the process of the test.
Test medicine: " 45% moroxydine+2% amino-oligosaccharide " solvable pulvis, patent applicant Guo Hong of the present invention just provides.Contrast medicament: 1. 2% amino-oligosaccharide aqua, commercially available product; 2. 20% moroxydine second copper wetting powder, commercially available product.
Test is handled and is set to 900 times of liquid of " 45% moroxydine+2% amino-oligosaccharide " solvable pulvis, 1200 times of liquid, 1500 times of liquid, 900 times of liquid of contrast medicament 2% amino-oligosaccharide aqua, 20% 400 times of liquid of moroxydine second copper wetting powder and 6 processing of clear water contrast, each is handled 4 times and repeats, totally 24 sub-districts, district's group is arranged at random.66.7 square metres of sub-district areas (long 9 meters of 4 row, row).
Watermelon virus disease initial phase begins spray medicine for the first time, with workers and peasants' 16 type manual sprayers, and injection diameter 0.7mm, 30 kilograms of every mu dispenser liquid later on every 7 days once, connect and spray 3 times.5 points are selected in every sub-district, and 5 strains are remembered in every some strain, investigation state of an illness radix before the dispenser, the incidence of 7 days investigation mark plant after the dispenser for the third time.Grade scale is as follows:
0 grade: complete stool is anosis;
1 grade: the slight floral leaf of young leaves
3 grades: young leaves floral leaf shape is obvious, slight shrinkage;
5 grades: the young leaves deformity, the shrinkage distortion, internode shortens;
7 grades: blade shank shape, stem is climing to attenuate, and the climing head of stem raises up, and growth stops.
Calculate preventive effect by above-mentioned formula (I and III).
2. test results and analysis
Result of the test is shown in Table 6:
The tomato virus disease initial phase begins medication, after 3 medications 7 days, 900 times of liquid of test medicine " 45% moroxydine+2% amino-oligosaccharide " solvable pulvis, 1200 times of liquid, three processing of 1500 times of liquid are respectively 78.1%, 73.4%, 70.5% to the average control efficiency of tomato virus disease, and 900 times of liquid of contrast medicament 2% amino-oligosaccharide aqua, 400 times of average control efficiency of liquid of 20% moroxydine second copper wetting powder are respectively 64.2%, 61.6%.Statistical results show, test medicine three are handled preventive effect and all extremely significantly are better than contrasting medicament.
Table 6 test medicine control watermelon virus disease result of the test
Embodiment 6 solubility fillers
The active ingredient of present embodiment formulation components: Moroxydine Hydrochloride 30%, amino-oligosaccharide 2.0%; 1. filler is respectively potassium chloride 68%, 2. calcium chloride 68%, 3. manganese sulphate 68%, 4. ammonium sulfate 68%, 5. potassium chloride 34%+ calcium chloride 34%, 6. urea 68%, 7. potassium humate 68%.
Compound method: select the single agent of above-mentioned each component granularity between 0.01～0.99mm, said components is mixed, can be processed into solvable pulvis.
1. test material and method
Test medicine is just provided by patent applicant Guo Hong of the present invention, and test is handled and is set to:
1. Moroxydine Hydrochloride 30%+ amino-oligosaccharide 2%+ potassium chloride 68%
2. Moroxydine Hydrochloride 30%+ amino-oligosaccharide 2%+ calcium chloride 68%
3. Moroxydine Hydrochloride 30%+ amino-oligosaccharide 2%+ manganese sulphate 68%
4. Moroxydine Hydrochloride 30%+ amino-oligosaccharide 2%+ ammonium sulfate 68%
5. Moroxydine Hydrochloride 30%+ amino-oligosaccharide 2%+ potassium chloride 34%+ calcium chloride 34%
6. Moroxydine Hydrochloride 30%+ amino-oligosaccharide 2%+ urea 68%
7. Moroxydine Hydrochloride 30%+ amino-oligosaccharide 2%+ potassium humate 68%
8. 2% amino-oligosaccharide aqua (commercially available product)
9. 20% moroxydine second copper wetting powder (commercially available product)
10. clear water contrast
Test is located at four Fang Xiang agrotechnical stations, Hua County, Henan Province, the subjects tomato virus disease, and kind is cooperation 901, seed is bought by market.To grow seedlings in seedling-cultivating tray after the tomato seeds vernalization, be transplanted to diameter during 3-4 sheet true leaf and be in 32 centimetres the flowerpot, every basin is planted 4 strains.Each handles plantation 8 basins, and per 2 basins are one group, repeat 4 times.After seedling is returned in transplanting, gather the virus disease blade from the field, extract viral juice, use juice rubbing manipulation virus inoculation disease then with the phosphate buffer of 0.1M.Inoculate after 3 days, each is handled and uses the atomizer for medicine even spraying, sprays once in 7 days at interval, sprays altogether 2 times.After the dispenser second time 7 days, each incidence of handling of investigation.State of an illness grade scale is as follows:
0 grade: complete stool is anosis;
1 grade: bright arteries and veins of blade and slight flower leaf paresthesia;
3 grades: blade has tangible flower leaf paresthesia;
5 grades: blade has heavy flower leaf paresthesia, and Zou contracts, and plant is slightly downgraded (below 1/3);
7 grades: the heavy floral leaf of blade, narrow down, plant is obviously downgraded (1/3-2/3), and petiole base has slight downright bad streak;
9 grades: leaf malformation, plant are seriously downgraded (more than 2/3), and base portion or growing point necrosis or complete stool are withered.
Calculate preventive effect by above-mentioned formula (I and II).
2. test results and analysis
Result of the test: be filler with potassium chloride, calcium chloride, manganese sulphate, ammonium sulfate, urea, potassium humate etc. respectively, " moroxydine 30%+ amino-oligosaccharide 2% " solvable pulvis of preparation, control tomato virus disease effect does not have significant difference.
Table 7 control tomato virus disease results from pot experiment test summary sheet
Results from pot experiment test shows, be filler with soluble inorganic salts such as potassium chloride, calcium chloride, manganese sulphate, ammonium sulfate, urea, potassium humate or organic matter respectively, " moroxydine 30%+ amino-oligosaccharide 2% " solvable pulvis of preparation, the effect of control tomato virus disease is 74.1%～77.3%, and contrast medicament 2% amino-oligosaccharide aqua, 20% moroxydine second copper wetting powder preventive effect are respectively 56.9%, 62.8%.Through significance of difference analysis, preventive effect does not have significant difference between the solvable pulvis of different filler preparations, all extremely significantly is better than contrasting medicament.
Claims (5)
1, a kind of viroses of plant control medicament is made up of the active principle of following weight portion: Moroxydine Hydrochloride 4.0～97.0; Amino-oligosaccharide 0.05～20.0.
2, control medicament as claimed in claim 1 is characterized in that, described viroses of plant control medicament comprises that also weight portion is: 2.0～96.0 solubility filler.
3, control medicament as claimed in claim 2 is characterized in that, described solubility filler is mineral salt or the organic matter that contains plant nutrient ingredient.
4, as the arbitrary described control medicament of claim 1-3, it is characterized in that the weight portion of described amino-oligosaccharide is: 0.1～15.0.
5, control medicament as claimed in claim 1 is characterized in that, it is solvable pulvis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100884707A CN101589721B (en) | 2009-07-02 | 2009-07-02 | Medicament for preventing and controlling plant virus diseases |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101971853A (en) * | 2010-09-28 | 2011-02-16 | 李学员 | Combined pesticide for controlling tobacco virus diseases |
| CN103598197A (en) * | 2013-12-05 | 2014-02-26 | 郭红甫 | Plant virus disease control medicament |
| CN103798272A (en) * | 2012-11-14 | 2014-05-21 | 陕西汤普森生物科技有限公司 | Bactericidal composition containing pyraclostrobin and moroxydine hydrochloride |
| CN104920466A (en) * | 2015-06-23 | 2015-09-23 | 郭红甫 | Corn crown rot prevention agent |
| CN106614690A (en) * | 2016-12-30 | 2017-05-10 | 新昌县拜特夫农业科技有限公司 | Reinforcing preparation for enhancing resistance to viral diseases of plants and application of reinforcing preparation |
| CN106818753A (en) * | 2017-01-03 | 2017-06-13 | 佛山市盈辉作物科学有限公司 | A kind of antiviral composition containing Chinese Stellera Root element and its application |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991006312A1 (en) * | 1989-10-27 | 1991-05-16 | Genencor International, Inc. | Protection of harvested crops from pathogens by treatment with enzymes |
| CN1064515C (en) * | 1996-10-07 | 2001-04-18 | 石家庄曙光制药厂 | Soluble prophylactico-therapeutic agent for plant virus |
| CN1225977C (en) * | 2002-12-12 | 2005-11-09 | 河北省农林科学院植物保护研究所 | Plant viral disease preventing and controlling agent and its prepn process |
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2009
- 2009-07-02 CN CN2009100884707A patent/CN101589721B/en active Active
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101971853A (en) * | 2010-09-28 | 2011-02-16 | 李学员 | Combined pesticide for controlling tobacco virus diseases |
| CN103798272A (en) * | 2012-11-14 | 2014-05-21 | 陕西汤普森生物科技有限公司 | Bactericidal composition containing pyraclostrobin and moroxydine hydrochloride |
| CN103798272B (en) * | 2012-11-14 | 2016-01-13 | 陕西汤普森生物科技有限公司 | The bactericidal composition of a kind of pyraclostrobin-containing and Moroxydine Hydrochloride |
| CN103598197A (en) * | 2013-12-05 | 2014-02-26 | 郭红甫 | Plant virus disease control medicament |
| CN103598197B (en) * | 2013-12-05 | 2015-07-22 | 郭红甫 | Plant virus disease control medicament |
| CN104920466A (en) * | 2015-06-23 | 2015-09-23 | 郭红甫 | Corn crown rot prevention agent |
| CN104920466B (en) * | 2015-06-23 | 2018-10-23 | 郭红甫 | A kind of pesticide control of corn top-rotten disease |
| CN106614690A (en) * | 2016-12-30 | 2017-05-10 | 新昌县拜特夫农业科技有限公司 | Reinforcing preparation for enhancing resistance to viral diseases of plants and application of reinforcing preparation |
| CN106818753A (en) * | 2017-01-03 | 2017-06-13 | 佛山市盈辉作物科学有限公司 | A kind of antiviral composition containing Chinese Stellera Root element and its application |
| CN106818753B (en) * | 2017-01-03 | 2018-08-31 | 佛山市盈辉作物科学有限公司 | A kind of antiviral composition containing Chinese Stellera Root element and its application |
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| Publication number | Publication date |
|---|---|
| CN101589721B (en) | 2012-07-25 |
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