CN101560536A - Fermentation method of microbial polysaccharide flocculant - Google Patents
Fermentation method of microbial polysaccharide flocculant Download PDFInfo
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- CN101560536A CN101560536A CNA2009100151640A CN200910015164A CN101560536A CN 101560536 A CN101560536 A CN 101560536A CN A2009100151640 A CNA2009100151640 A CN A2009100151640A CN 200910015164 A CN200910015164 A CN 200910015164A CN 101560536 A CN101560536 A CN 101560536A
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Abstract
The invention provides a fermentation method of a microbial polysaccharide flocculant, comprising the steps of choosing arthrobacter LF-Tou2 as a producing strain of the microbial polysaccharide flocculant and adopting cascaded strain propagation and fermentation methods to produce the high-efficient microbial polysaccharide flocculant used for water quality purification. The microbial polysaccharide flocculant of the invention is non-toxic and biodegradable, has the advantages of low production cost, wide application range, high service efficiency and no secondary pollution when being used, has comprehensive functions of removing inorganic ions, reducing contents of organic matters and adsorbing heavy metals, is applied in such technical fields as purification of quality of supplied water, treatment of inland lake water and sewage quality, separation of biological products in the fermentation industry and the like, and has very obvious economic and social effects.
Description
Technical field
The present invention relates to a kind of preparation method of water conditioner, belong to modern microbial engineering field, specifically a kind of fermentation process of microbial polysaccharide flocculant.
Background technology
The fermentation process that uses in present microbial fermentation research and suitability for industrialized production has batch culture, cultured continuously and fed batch cultivation method, has their own characteristics each.The inorganic salts flocculation agent that in feedwater and draining etc., uses, as, Tai-Ace S 150, aluminum chloride, iron(ic) chloride etc., its treatment effect is undesirable; High molecular polymerization inorganic salts flocculation agent, as, polymerize aluminum chloride, bodied ferric sulfate etc. have obtained effect preferably, but in use can produce certain secondary pollution to environment in water treatment, and the secondary pollution that aluminium system produces is more obvious.The aluminum ion of water middle and high concentration is harmful to hydrobiont and plant, and finally by food chain and tap water harm humans health, wherein senile dementia disease is exactly owing to due to the aluminium too high levels in the brain.Organic polymer coargulator, as, polyacrylamide, its residue are difficult for by biological degradation, and be unfavorable to environment and human health, has secondary pollution, and particularly its monomer has intensive neurotoxin and carcinogenesis.Therefore, good, the applied range of research and development flocculating effect, readily biodegradable, environmentally safe biological flocculant become the certainty of development.That the flocculation agent of microorganisms has is nontoxic, have biological degradability, raw materials for production such as are easy to get, can be mass-produced at advantage, are inexhaustible Biological resources.With regard to the bacterium for producing flocculant of microbe kind of present report, all exist the deficiency of fermentation period length, raw materials cost and production cost height, waste water resource to some extent.
Summary of the invention
The objective of the invention is to overcome the deficiency of above microbial flocculant on fermentation method for producing, provide a kind of with short production cycle, raw materials cost and production cost is low, the conserve water resource, and be suitable for the fermentation process of the microbial polysaccharide flocculant of large-scale industrial production and industrial applications.
The objective of the invention is to realize in the following manner, this method comprises that preparation, fermentation culture and the liquid waste disposal circulating fermentation of substratum use.
The polysaccharide flocculant with microbial fermentation processes production that the present invention relates to is to have the anionic biological polymer of amino positively charged ion, carboxyl and hydroxyl, gathering and adsorptive power are big, the flocculation function is strong, are a kind of novel water quality treatment agents of representing the flocculation agent developing direction.Method of the present invention serves as to produce bacterial classification with Arthrobacter LF-Tou2, the preparation of bacterial classification was done detailed description in the patent No. for the patent documentation of " ZL200310105515.X ", adopt LF-Tou2 for producing bacterial classification, prepare the industrial microbe polysaccharide flocculant through fermentation culture method of the present invention.
Select for use Arthrobacter LF-Tou2 for producing bacterial classification, use the step of microbial polysaccharide flocculant as follows through fermentation culture production water treatment:
A) composition of fermention medium
Fermention medium is made up of following materials of weight proportions: Dian Fentang 15-25 part, (NH
4)
2SO
41-5 part, K
2HPO
41-8 part, MgSO
40.1-1 part, CaCl
20.2-1.5 part, corn steep liquor 0.1-1.5 part, 1000 parts in water;
The preparation process of fermention medium is as follows:
Get (NH
4)
2SO
4Be dissolved in 150 parts of water standby, Dian Fentang, K
2HPO
4, MgSO
4, CaCl
2With corn steep liquor be dissolved in 850 parts of water standby, two kinds of solution respectively by add behind 120 ℃ of continuous sterilization 8min mix in the fermentor tank standby:
B) fermentation process and fermentation condition control
Adopt straight leaf turbine type air blow tank, adopt connection level bacterial classification propagation fermentation process to ferment, the fermentor tank two-stage series connection, fermentor tank 1 batch charging coefficient is 70%-78%, inoculum size is 2%-10%; Fermentor tank 1 is cultivated 8-14h, and the 85%-95% of nutrient solution is wherein forwarded in the fermentor tank 2, adds fresh culture multiplication culture again simultaneously in fermentor tank 1, and fermentor tank 2 continues to be cultured to fermentation ends.The culture condition of two fermentations jar control is that temperature 20-32 ℃, mixing speed 100-250 rev/min, tank pressure are 0.01-0.03MPa;
C) fermentation waste water cyclically utilizing
Fermentation ends send the ultra-filtration membrane unit that fermented liquid is carried out filtering separation the fermented liquid in the fermentor tank 2, obtain ultrafiltration and see through liquid, ultrafiltration is seen through liquid be mixed for joining foster base with the ratio of 20%-60% and the water that ferments, after recycling 6 times, with nanofiltration membrane ultrafiltration is seen through liquid and carry out nanofiltration acquisition fermentation water, realize that the filtrate cycle utilization reaches zero release.
Fermention medium is made up of following materials of weight proportions: Dian Fentang 18-22 part, (NH
4)
2SO
42-4 part, K
2HPO
43-6 part, MgSO
40.3-0.8 part, CaCl
20.5-1.2 part, corn steep liquor 0.4-1.2 part, 1000 parts in water.
Fermention medium is made up of following materials of weight proportions: 20 parts of Dian Fentangs, (NH
4)
2SO
43 parts, K
2HPO
45 parts, MgSO
40.6 part, CaCl
20.8 part, 0.8 part of corn steep liquor, 1000 parts in water.
The fermentation water is clear water or sees through liquid or filter ultrafiltration through the filtrate behind the liquid with nanofiltration membrane with the ultrafiltration after the ultrafiltration membrance filter fermented liquid.
Specific embodiment
Select for use Arthrobacter LF-Tou2 for producing bacterial classification, use the step of microbial polysaccharide flocculant as follows through fermentation culture production water treatment:
A) preparation of fermention medium
The quality of fermention medium is formed (part):
Dian Fentang 15-25, (NH
4)
2SO
41-5, K
2HPO
41-8, MgSO
40.1-1, CaCl
20.2-1.5, corn steep liquor 0.1-1.5, water 1000.
Get 15% fermentation water and be used for preparation (NH
4)
2SO
4Composition, 85% fermentation water is used to prepare other composition, respectively by behind 120 ℃ of continuous sterilization 8min in fermentor tank mixing for standby use:
B) fermentation process and fermentation condition control fermentation process and fermentation condition control
Adopt straight leaf turbine type air blow tank, adopt connection level bacterial classification propagation fermentation process to ferment, the fermentor tank two-stage series connection, fermentor tank 1 batch charging coefficient is 70%-78%, inoculum size is 2%-10%; Fermentor tank 1 is cultivated 8-14h, and the 85%-95% of nutrient solution is wherein forwarded in the fermentor tank 2, adds fresh culture multiplication culture again simultaneously in fermentor tank 1, and fermentor tank 2 continues to be cultured to fermentation ends.The culture condition of two fermentations jar control is that temperature 20-32 ℃, mixing speed 100-250 rev/min, tank pressure are 0.01-0.03MPa;
C) fermentation waste water cyclically utilizing
Fermentation ends send the ultra-filtration membrane unit that fermented liquid is carried out filtering separation the fermented liquid in the fermentor tank 2, obtain ultrafiltration and see through liquid, ultrafiltration is seen through liquid be mixed for joining foster base with the ratio of 20%-60% and the water that ferments, after recycling 6 times, with nanofiltration membrane ultrafiltration is seen through liquid and carry out nanofiltration acquisition fermentation water, realize that the filtrate cycle utilization reaches zero release.
D) equipment of fermentation step and technical process
The equipment that fermentation step relates to has fermentor tank, material-compound tank, aseptic culture medium storage tank, fermented liquid storage tank, ultra-filtration membrane unit, nanofiltration membrane etc., and technical process is as shown below:
The fermentation process of microbial polysaccharide flocculant of the present invention compare with the fermentation process of various biological flocculants in the prior art have that fermentation period is short, raw materials cost and production cost is low, water resource utilization efficiency is high and do not have characteristics such as fermentation discharge of wastewater, have important engineering meaning and remarkable economical and be worth.
A) preparation of fermention medium
The quality of fermention medium is formed (g/L):
Dian Fentang 20, (NH
4)
2SO
44, K
2HPO
46, MgSO
40.8, CaCl
21.3, corn steep liquor 1.3, water 1000.
Get 15% fermentation water and be used for preparation (NH
4)
2SO
4Composition, 85% fermentation water water is used to prepare other composition, respectively by behind 120 ℃ of continuous sterilization 8min in fermentor tank mixing for standby use:
B) fermentation process and fermentation condition control
Adopt straight leaf turbine type air blow tank, fermentation process adopts connection level bacterial classification propagation fermentation process, and fermentor tank 1 batch charging coefficient is 75%, and inoculum size is 8%.The fermentor tank two-stage series connection, fermentor tank 1 is cultivated 13h, forwards 90% of nutrient solution to fermentor tank 2, adds fresh culture multiplication culture again simultaneously in fermentor tank 1, and fermentor tank 2 continues to be cultured to fermentation ends.The culture condition of two fermentations jar control is that 30 ℃ of temperature, 220 rev/mins of mixing speed, tank pressure are 0.02MPa.
C) fermentation waste water cyclically utilizing
The ultrafiltration that the ultra-filtration membrane unit concentrate is produced the fermented liquid ultra-filtration and separation sees through liquid and is mixed for joining with the fermentation water with 50% ratio and supports base, recycle 6 times after, with nanofiltration membrane ultrafiltration is carried out nanofiltration through liquid and obtains the water that ferments again.Through the use of fermentation waste water cyclically utilizing technology, fermentation waste water reaches zero release.
A) quality of the preparation fermention medium of fermention medium is formed (g/L):
Dian Fentang 15, (NH
4)
2SO
43, K
2HPO
44, MgSO
40.5, CaCl
21.0, corn steep liquor 0.9, water 1000.
Get 15% fermentation water and be used for preparation (NH
4)
2SO
4Composition, 85% fermentation water water is used to prepare other composition, respectively by behind 120 ℃ of continuous sterilization 8min in fermentor tank mixing for standby use:
B) fermentation process and fermentation condition control
Adopt straight leaf turbine type air blow tank, fermentation process adopts bacterial classification propagation fermentation method on the throne, and fermentor tank 1 batch charging coefficient is 70%, and inoculum size is 5%.The fermentor tank two-stage series connection, fermentor tank 1 is cultivated 10h, forwards 85% of nutrient solution to fermentor tank 2, adds fresh culture multiplication culture again simultaneously in fermentor tank 1, and fermentor tank 2 continues to be cultured to fermentation ends.The culture condition of two fermentations jar control is that 25 ℃ of temperature, 180 rev/mins of mixing speed, tank pressure are 0.01MPa.
C) fermentation waste water cyclically utilizing
The ultrafiltration that the ultra-filtration membrane unit concentrate is produced the fermented liquid ultra-filtration and separation sees through liquid and is mixed for joining with the fermentation water with 40% ratio and supports base, recycle 6 times after, with nanofiltration membrane ultrafiltration is carried out nanofiltration through liquid and obtains the water that ferments again.Through the use of fermentation waste water cyclically utilizing technology, fermentation waste water reaches zero release.
Use the microbial polysaccharide flocculant of method fermentation of the present invention to possess low, the assurance of fermentation step production cost Fermentation high yield and save the fermentation water, be convenient to the characteristics such as automation control, for the subsequent technique process by zymotic fluid The microbial polysaccharide flocculant that obtains high extraction is laid a good foundation.
Claims (4)
1. the fermentation process of a microbial polysaccharide flocculant is characterized in that, selects for use Arthrobacter LF-Tou2 for producing bacterial classification, and through fermentation culture production water treatment microbial polysaccharide flocculant, the fermentation of this biological flocculant may further comprise the steps:
A) composition of fermention medium
Fermention medium is made up of following materials of weight proportions: Dian Fentang 15-25 part, (NH
4)
2SO
41-5 part, K
2HPO
41-8 part, MgSO
40.1-1 part, CaCl
20.2-1.5 part, corn steep liquor 0.1-1.5 part, 1000 parts in water;
The preparation process of fermention medium is as follows:
Get (NH
4)
2SO
4Be dissolved in 150 parts of water standby, Dian Fentang, K
2HPO
4, MgSO
4, CaCl
2With corn steep liquor be dissolved in 850 parts of water standby, two kinds of solution respectively by add behind 120 ℃ of continuous sterilization 8min mix in the fermentor tank standby:
B) fermentation process and fermentation condition control
Adopt straight leaf turbine type air blow tank, adopt connection level bacterial classification propagation fermentation process to ferment, the fermentor tank two-stage series connection, fermentor tank 1 batch charging coefficient is 70%-78%, inoculum size is 2%-10%; Fermentor tank 1 is cultivated 8-14h, and the 85%-95% of nutrient solution is wherein forwarded in the fermentor tank 2, adds fresh culture multiplication culture again simultaneously in fermentor tank 1, and 2 of fermentor tanks continue to be cultured to fermentation ends.The culture condition of two fermentations jar control is that temperature 20-32 ℃, mixing speed 100-250 rev/min, tank pressure are 0.01-0.03MPa;
C) fermentation waste water cyclically utilizing
Fermentation ends send the ultra-filtration membrane unit that fermented liquid is carried out filtering separation the fermented liquid in the fermentor tank 2, obtain ultrafiltration and see through liquid, ultrafiltration is seen through liquid be mixed for joining foster base with the ratio of 20%-60% and the water that ferments, after recycling 6 times, with nanofiltration membrane ultrafiltration is seen through liquid and carry out nanofiltration acquisition fermentation water, realize that the filtrate cycle utilization reaches zero release.
2, method according to claim 1 is characterized in that, fermention medium is made up of following materials of weight proportions: Dian Fentang 18-22 part, (NH
4)
2SO
42-4 part, K
2HPO
43-6 part, MgSO
40.3-0.8 part, CaCl
20.5-1.2 part, corn steep liquor 0.4-1.2 part, 1000 parts in water.
3, method according to claim 1 is characterized in that, fermention medium is made up of following materials of weight proportions: 20 parts of Dian Fentangs, (NH
4)
2SO
43 parts, K
2HPO
45 parts, MgSO
40.6 part, CaCl
20.8 part, 0.8 part of corn steep liquor, 1000 parts in water.
4, method according to claim 1 is characterized in that, the fermentation water is clear water or sees through liquid or filter ultrafiltration through the filtrate behind the liquid with nanofiltration membrane with the ultrafiltration after the ultrafiltration membrance filter fermented liquid.
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|---|---|---|---|
| CNA2009100151640A CN101560536A (en) | 2009-05-19 | 2009-05-19 | Fermentation method of microbial polysaccharide flocculant |
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|---|---|---|---|
| CNA2009100151640A CN101560536A (en) | 2009-05-19 | 2009-05-19 | Fermentation method of microbial polysaccharide flocculant |
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|---|---|
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870958A (en) * | 2010-05-10 | 2010-10-27 | 新疆农业科学院微生物应用研究所 | Paenibacillus and application thereof to environmental engineering |
| CN104528952A (en) * | 2014-07-21 | 2015-04-22 | 济南大学 | Compound microbial flocculant processing strong-alkali waste water without calcium ions and application thereof |
| CN106865804A (en) * | 2017-03-23 | 2017-06-20 | 福建凯立生物制品有限公司 | A kind of Zhongshengmycin mother medicine clean preparation method |
| CN113430126A (en) * | 2021-08-11 | 2021-09-24 | 栾兴社 | Aureobasidium pullulans and method for preparing melanin polysaccharide by using same |
-
2009
- 2009-05-19 CN CNA2009100151640A patent/CN101560536A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101870958A (en) * | 2010-05-10 | 2010-10-27 | 新疆农业科学院微生物应用研究所 | Paenibacillus and application thereof to environmental engineering |
| CN104528952A (en) * | 2014-07-21 | 2015-04-22 | 济南大学 | Compound microbial flocculant processing strong-alkali waste water without calcium ions and application thereof |
| CN106865804A (en) * | 2017-03-23 | 2017-06-20 | 福建凯立生物制品有限公司 | A kind of Zhongshengmycin mother medicine clean preparation method |
| CN106865804B (en) * | 2017-03-23 | 2020-08-18 | 福建凯立生物制品有限公司 | Clean production method of Zhongshengmycin mother medicine |
| CN113430126A (en) * | 2021-08-11 | 2021-09-24 | 栾兴社 | Aureobasidium pullulans and method for preparing melanin polysaccharide by using same |
| CN113430126B (en) * | 2021-08-11 | 2023-05-26 | 栾兴社 | Aureobasidium pullulans and method for preparing melanin polysaccharide by using same |
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Open date: 20091021 |