CN101560252A - Method for extracting zein by microwave - Google Patents
Method for extracting zein by microwave Download PDFInfo
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- CN101560252A CN101560252A CNA2009100393298A CN200910039329A CN101560252A CN 101560252 A CN101560252 A CN 101560252A CN A2009100393298 A CNA2009100393298 A CN A2009100393298A CN 200910039329 A CN200910039329 A CN 200910039329A CN 101560252 A CN101560252 A CN 101560252A
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Abstract
The invention discloses a method for extracting zein by microwave. The method comprises the following steps: corn protein powder is used as a raw material; complex enzyme is added step by step into the powder for treatment, and the obtained power is filtered; the filter residue is added into ethanol aqueous solution to be subjected to microwave extraction, and is centrifugally separated to obtain zein extract; water is added into the zein extract to dilute the ethanol concentration of the zein extract to obtain zein precipitate; finally, the zein precipitate is dried to obtain a zein finished product. The method adopts double enzymolys method to carry out pretreatment and microwave technology to carry out extraction, has the characteristics of high yield, high purity, low production cost and short production period, significantly improves the additional value of the corn protein powder, and contributes to the comprehensive development and utilization of the corn protein powder.
Description
Technical field
The present invention relates to a kind of extracting method of zein.
Background technology
Zein powder claims maize yellow-powder again, is the by product with Maize Production starch.The protein content of this class Zein powder is about 60%, but owing to lack essential amino acids such as Methionin, tryptophane, its biological value is low, how to sell at present as the low value feedstuff protein, a lot of factories even do not do any utilization, let alone with discharging of waste liquid, both caused the waste of resource, serious again pollution environment.
The zein that the present invention relates to is the main protein in the Zein powder.Its water insoluble and anhydrous alcohols dissolves in the alcohol solution of 60-95%, can also be dissolved in the organic solvents such as highly basic and acetone, acetic acid.Zein solution has film-forming properties, and after solvent evaporates, protein condenses can form maize alcohol-soluble protein film smooth, transparent and that have characteristics such as resistance is moist, resistance oxygen, enteric solubility.Because zein is water insoluble, lack Methionin and tryptophane, and have color and luster and smell problem, thereby lessly use as food raw material, but solvability that it is unique and film-forming properties make it have vast market prospect in fields such as medical and health and food chemical industry.At present, zein mainly is as pharmaceutical excipient, as tablet coating and slowly-releasing wall material, also can be applicable to aspects such as the fresh-keeping coating of candy dressing, fruit and meat products, grease embedding wall material, biodegradable plastic, tackiness agent.
Summary of the invention
The method that the purpose of this invention is to provide a kind of extracting zein by microwave, this method yield height, the purity height, production cost is low, and is with short production cycle.
Purpose of the present invention is achieved by taking following technical scheme:
A kind of method of extracting zein by microwave, it is characterized in that, with the Zein powder is raw material, add the prozyme proceed step by step and handle, filter, filter residue is added aqueous ethanolic solution, microwave extraction, centrifugation gets the zein extracting solution, and the alcohol concn of thin up zein extracting solution gets the zein precipitation, and last drying gets the zein finished product.Specifically may further comprise the steps:
(1) pre-treatment: with the Zein powder is raw material, add water, adjust pH5.0～6.5, add α-Dian Fenmei, 65～90 ℃ were reacted 1.5～6 hours, be warmed up to 120～140 ℃ of enzymes that go out then, treat to adjust pH3.0～5.0 after temperature drops to room temperature, add saccharifying enzyme, 45～60 ℃ were reacted 1～4 hour, be warmed up to 90～110 ℃ of enzymes that go out again, filter, discard treatment solution;
(2) extract: filter residue is added aqueous ethanolic solution, microwave extraction;
(3) centrifugal: centrifugation, gained solution are the zein extracting solution;
(4) precipitation: the alcohol concn of thin up zein extracting solution, leave standstill, get the zein precipitation;
(5) drying: zein precipitates through vacuum or forced air drying, gets the zein finished product.
The weight ratio of Zein powder and water is 1: 4～10 in the described step (1).
The add-on of α-Dian Fenmei is 0.1～0.5% of a Zein powder weight in the described step (1); The add-on of saccharifying enzyme is 0.2～1.0% of a Zein powder weight.
The weight concentration of aqueous ethanolic solution is 70～90% in the described step (2).
The solid-liquid ratio of filter residue and aqueous ethanolic solution is 1 kilogram in the described step (2): 6～14 liters.
The microwave extraction condition is 100～500 watts of microwave powers in the described step (2), 3～15 minutes extraction times.
Add volume of water in the described step (4) and be 2.5～5 times of zein extracting liquid volume.
Time of repose is 2～12 hours in the described step (4).
Drying conditions is 50～100 ℃ of temperature in the described step (5), 4～20 hours time.
Compared with prior art, the present invention has following characteristics:
(1) the present invention adopts α-Dian Fenmei and saccharifying enzyme to carry out pre-treatment, can significantly reduce the impurity in the product, improves the purity of product.
(2) the present invention adopts microwave technology to extract, and extraction yield is low when having solved with the extraction of common heating method, extraction time long, the energy consumption problem of higher.
(3) the present invention has significantly improved the added value of Zein powder, helps the comprehensive development and utilization of Zein powder.
Embodiment
Below by specific embodiment the present invention is set forth, but protection scope of the present invention is not limited thereto.The person of an ordinary skill in the technical field all can realize purpose of the present invention according to above content disclosed by the invention.
Embodiment 1
Take by weighing Zein powder 200g, add 1200 ml waters and mix.Adjust pH5.5 ± 0.1, add 0.3% α-Dian Fenmei, 75 ℃ were reacted 4 hours.Be warmed up to 130 ℃ of enzymes that go out, treat to adjust pH4.0 ± 0.1 after temperature drops to room temperature, add 0.4% saccharifying enzyme, 50 ℃ were reacted 2 hours.Be warmed up to 100 ℃ of enzymes that go out, filter, filter residue adds 1600 milliliter of 70% ethanolic soln, microwave extraction 10 minutes, and microwave power is 200 watts.Centrifugal zein extracting solution adds the alcohol concn that 4800 milliliters water dilutes the zein extracting solution, leaves standstill 5 hours, get the zein precipitation, 80 ℃ of forced air dryings got the zein product in 12 hours, and yield is 21.3%, protein content 91.2%.
Embodiment 2
Take by weighing Zein powder 150g, add 600 ml waters and mix.Adjust pH6.0 ± 0.1, add 0.2% α-Dian Fenmei, 90 ℃ were reacted 2 hours.Be warmed up to 130 ℃ of enzymes that go out, treat to adjust pH4.5 ± 0.1 after temperature drops to room temperature, add 0.6% saccharifying enzyme, 45 ℃ were reacted 2 hours.Be warmed up to 100 ℃ of enzymes that go out, filter, filter residue adds 1500 milliliter of 85% ethanolic soln, microwave extraction 8 minutes, and microwave power is 300 watts.Centrifugal zein extracting solution adds the alcohol concn that 6000 milliliters water dilutes the zein extracting solution, leaves standstill 3 hours, get the zein precipitation, 100 ℃ of forced air dryings got the zein product in 6 hours, and yield is 23.8%, protein content 92.4%.
Embodiment 3
Take by weighing Zein powder 150g, add 1200 ml waters and mix.Adjust pH6.2 ± 0.1, add 0.2% α-Dian Fenmei, 75 ℃ were reacted 2.5 hours.Be warmed up to 140 ℃ of enzymes that go out, treat to adjust pH4.8 ± 0.1 after temperature drops to room temperature, add 0.7% saccharifying enzyme, 45 ℃ were reacted 2.5 hours.Be warmed up to 110 ℃ of enzymes that go out, filter, filter residue adds 1200 milliliter of 90% ethanolic soln, microwave extraction 3 minutes, and microwave power is 500 watts.Centrifugal zein extracting solution adds the alcohol concn that 6000 milliliters water dilutes the zein extracting solution, leaves standstill 10 hours, get the zein precipitation, 80 ℃ of vacuum-dryings got the zein product in 6 hours, and yield is 23.1%, protein content 89.7%.
Embodiment 4
Take by weighing Zein powder 100g, add 600 ml waters and mix.Adjust pH5.3 ± 0.1, add 0.4% α-Dian Fenmei, 85 ℃ were reacted 3 hours.Be warmed up to 120 ℃ of enzymes that go out, treat to adjust pH3.5 ± 0.1 after temperature drops to room temperature, add 0.4% saccharifying enzyme, 60 ℃ were reacted 3 hours.Be warmed up to 95 ℃ of enzymes that go out, filter, filter residue adds 1200 milliliter of 85% ethanolic soln, microwave extraction 13 minutes, and microwave power is 200 watts.Centrifugal zein extracting solution adds the alcohol concn that 4800 milliliters water dilutes the zein extracting solution, leaves standstill 8 hours, get the zein precipitation, 60 ℃ of vacuum-dryings got the zein product in 10 hours, and yield is 22.4%, protein content 91.8%.
Claims (10)
1, a kind of method of extracting zein by microwave, it is characterized in that, with the Zein powder is raw material, add the prozyme proceed step by step and handle, filter, filter residue is added aqueous ethanolic solution, microwave extraction, centrifugation gets the zein extracting solution, and the alcohol concn of thin up zein extracting solution gets the zein precipitation, and last drying gets the zein finished product.
2, the method for extracting zein by microwave according to claim 1 is characterized in that, may further comprise the steps:
(1) pre-treatment: with the Zein powder is raw material, add water, adjust pH5.0～6.5, add α-Dian Fenmei, 65～90 ℃ were reacted 1.5～6 hours, be warmed up to 120～140 ℃ of enzymes that go out then, treat to adjust pH3.0～5.0 after temperature drops to room temperature, add saccharifying enzyme, 45～60 ℃ were reacted 1～4 hour, be warmed up to 90～110 ℃ of enzymes that go out again, filter, discard treatment solution;
(2) extract: filter residue is added aqueous ethanolic solution, microwave extraction;
(3) centrifugal: centrifugation, gained solution are the zein extracting solution;
(4) precipitation: the alcohol concn of thin up zein extracting solution, leave standstill, get the zein precipitation;
(5) drying: zein precipitates through vacuum or forced air drying, gets the zein finished product.
3, the method for extracting zein by microwave according to claim 2 is characterized in that, the weight ratio of Zein powder and water is 1: 4～10 in the described step (1).
4, the method for extracting zein by microwave according to claim 2 is characterized in that, the add-on of α-Dian Fenmei is 0.1～0.5% of a Zein powder weight in the described step (1); The add-on of saccharifying enzyme is 0.2～1.0% of a Zein powder weight.
5, the method for extracting zein by microwave according to claim 2 is characterized in that, the weight concentration of aqueous ethanolic solution is 70～90% in the described step (2).
6, the method for extracting zein by microwave according to claim 2 is characterized in that, the solid-liquid ratio of filter residue and aqueous ethanolic solution is 1 kilogram in the described step (2): 6～14 liters.
7, the method for extracting zein by microwave according to claim 2 is characterized in that, the microwave extraction condition is 100～500 watts of microwave powers in the described step (2), 3～15 minutes extraction times.
8, the method for extracting zein by microwave according to claim 2 is characterized in that, adds volume of water in the described step (4) and be 2.5～5 times of zein extracting liquid volume.
9, the method for extracting zein by microwave according to claim 2 is characterized in that, time of repose is 2～12 hours in the described step (4).
10, the method for extracting zein by microwave according to claim 2 is characterized in that, drying conditions is 50～100 ℃ of temperature in the described step (5), 4～20 hours time.
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| CN2009100393298A CN101560252B (en) | 2009-05-08 | 2009-05-08 | Method for extracting zein by microwave |
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| CN2009100393298A CN101560252B (en) | 2009-05-08 | 2009-05-08 | Method for extracting zein by microwave |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102532294A (en) * | 2011-12-28 | 2012-07-04 | 广东省食品工业研究所 | Method for extracting zein |
| CN103073914A (en) * | 2013-02-05 | 2013-05-01 | 山东省农业科学院农产品研究所 | Method for extracting maize yellow pigment from maize protein |
| CN105361168A (en) * | 2015-12-02 | 2016-03-02 | 绥化学院 | Composite antioxidation peptides and preparation method thereof |
| CN106009766A (en) * | 2016-05-26 | 2016-10-12 | 中国科学院兰州化学物理研究所 | Method for continuously extracting maize yellow pigment and zein from maize protein meal |
| CN109207122A (en) * | 2018-06-28 | 2019-01-15 | 滁州市经纬装备科技有限公司 | A kind of preparation method for the flexible material absorbing radar signal |
| WO2019060179A1 (en) * | 2017-09-21 | 2019-03-28 | Cargill, Incorporated | Corn protein retention during extraction |
| US11375736B2 (en) | 2016-03-24 | 2022-07-05 | Cargill, Incorporated | Corn protein product having decreased free sulfite levels and method for manufacturing same |
| US11980217B2 (en) | 2017-08-02 | 2024-05-14 | Cargill, Incorporated | Extruded corn protein material |
| US11985990B2 (en) | 2016-09-23 | 2024-05-21 | Cargill, Incorporated | Corn protein retention during extraction |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103059116A (en) * | 2012-12-24 | 2013-04-24 | 广东省食品工业研究所 | Production method of white zein |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1570127A (en) * | 2003-07-23 | 2005-01-26 | 东北农业大学 | High F value oligopeptide production method by corn protein enzymolysis |
| CN1285731C (en) * | 2004-12-22 | 2006-11-22 | 华南理工大学 | Method for enzyme hydrolyzing zein through two steps method |
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2009
- 2009-05-08 CN CN2009100393298A patent/CN101560252B/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102532294A (en) * | 2011-12-28 | 2012-07-04 | 广东省食品工业研究所 | Method for extracting zein |
| CN103073914A (en) * | 2013-02-05 | 2013-05-01 | 山东省农业科学院农产品研究所 | Method for extracting maize yellow pigment from maize protein |
| CN103073914B (en) * | 2013-02-05 | 2014-06-04 | 山东省农业科学院农产品研究所 | Method for extracting maize yellow pigment from maize protein |
| CN105361168A (en) * | 2015-12-02 | 2016-03-02 | 绥化学院 | Composite antioxidation peptides and preparation method thereof |
| US11375736B2 (en) | 2016-03-24 | 2022-07-05 | Cargill, Incorporated | Corn protein product having decreased free sulfite levels and method for manufacturing same |
| CN106009766B (en) * | 2016-05-26 | 2017-11-17 | 中国科学院兰州化学物理研究所 | A kind of method that corn yellow OB and zeins are continuously extracted from corn protein powder |
| CN106009766A (en) * | 2016-05-26 | 2016-10-12 | 中国科学院兰州化学物理研究所 | Method for continuously extracting maize yellow pigment and zein from maize protein meal |
| US11985990B2 (en) | 2016-09-23 | 2024-05-21 | Cargill, Incorporated | Corn protein retention during extraction |
| US11980217B2 (en) | 2017-08-02 | 2024-05-14 | Cargill, Incorporated | Extruded corn protein material |
| WO2019060179A1 (en) * | 2017-09-21 | 2019-03-28 | Cargill, Incorporated | Corn protein retention during extraction |
| CN111132557A (en) * | 2017-09-21 | 2020-05-08 | 嘉吉公司 | Zein retention during extraction |
| US11667670B2 (en) | 2017-09-21 | 2023-06-06 | Cargill, Incorporated | Corn protein retention during extraction |
| CN111132557B (en) * | 2017-09-21 | 2023-06-30 | 嘉吉公司 | Corn protein retention during extraction |
| CN109207122A (en) * | 2018-06-28 | 2019-01-15 | 滁州市经纬装备科技有限公司 | A kind of preparation method for the flexible material absorbing radar signal |
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| CN101560252B (en) | 2012-01-11 |
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Address after: 510308 No. 146 East Xingang Road, Guangzhou, Guangdong, Haizhuqu District Patentee after: Guangdong Food Industry Research Institute Co Ltd Address before: 510308 No. 146 East Xingang Road, Guangdong, Guangzhou Patentee before: Guangdong Prov. Food Industry Inst. |
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