CN101524446A - Process for preparing grape seed extract - Google Patents
Process for preparing grape seed extract Download PDFInfo
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- CN101524446A CN101524446A CN200810030763A CN200810030763A CN101524446A CN 101524446 A CN101524446 A CN 101524446A CN 200810030763 A CN200810030763 A CN 200810030763A CN 200810030763 A CN200810030763 A CN 200810030763A CN 101524446 A CN101524446 A CN 101524446A
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- Prior art keywords
- vitis viniferae
- semen vitis
- dry
- extracting solution
- preparation technology
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Abstract
The invention discloses a process for preparing grape seed extract, which comprises the following steps of: adding 95 to 100 DEG C water in an amount of 5 to 8 times of that of grape seeds for extracting for 2 to 3 times at an interval of [each for] 2 to 3 hours to obtain extract liquid; allowing the extract liquid to cool to 20 to 40 DEG C, passing the extract liquid through macroporous resin, and eluting the macroporous resin with 60 to 80 percent alcohol; reclaiming the alcohol from eluent, concentrating the eluent and drying the eluent; subjecting an obtained dry product to 1 to 3 times of refluxing extraction with 90 to 97 percent alcohol in an amount of 5 to 8 times of that of the dry product at an interval of 2 to 3 hours; and reclaiming the alcohol from extract liquid, concentrating the extract liquid, and drying the extract liquid to obtain the product. The process can extract an OPC ingredient completely and effectively, and the product obtained by the process has stable and reliable quality.
Description
Technical field
The present invention relates to a kind of preparation technology of Semen Vitis viniferae extract.
Background technology
OPC (Oligomeric Proanthocyanidins Complexes) is meant procyanidolic oligomers.It is the strongest antioxidation of finding in the current medical research that has, and eliminates the material of free radical and anti-aging effects.Research worker exists " procyanidin OPC " through research back confirmation repeatedly in the Semen Vitis viniferae, it can effectively remove unnecessary freedom in the body, and oxidation resistance is 50 times of vitamin E, ascorbic 20 times.At present, its oxidation resistance has obtained internationally recognized, and it is the human life necessary, and not second to various vitamin, and human body can not produce voluntarily, can not lack a moment again to the importance of human body.Thereby how from Semen Vitis viniferae, to extract the OPC composition fully effectively, be a major issue of the required solution of technology researcher.
Summary of the invention
Purpose of the present invention aims to provide a kind of preparation technology of Semen Vitis viniferae extract, can carry out fully effectively extracting to the OPC composition, and the stable and reliable product quality of gained.
The objective of the invention is to realize by following manner:
Technology of the present invention may further comprise the steps:
1) add that Semen Vitis viniferae 5-8 doubly measures, temperature is that 95-100 ℃ water circulates and extracts 2-3 time, 2-3 hour at every turn, obtains extracting solution;
2) extracting solution is cooled to 20-40 ℃ and crosses macroporous resin;
3) the ethanol elution macroporous resin of usefulness 60-80%;
4) with 3) after resulting eluent of step reclaims ethanol, concentrate, dry;
5) with 4) alcohol reflux 1-3 time of the 5-8 times of 90-97% of dry thing reuse of step gained, 2-3 hour at every turn;
6) after extracting solution reclaims ethanol, concentrated, dry, promptly get product.
Of the present invention 2) in the step preferably with 70% ethanol elution;
Of the present invention 4), 6) temperature of concentrate drying is 70-90 ℃ in the step, is preferably 80 ℃.
Of the present invention 5) dry thing is preferably used 6 times of amounts, 95% alcohol reflux 2 times, each 2 hours in the step.
The OPC of the extract of the gained that is made by technology of the present invention, polyphenol content can reach more than 95%.
The specific embodiment
Following examples are intended to illustrate the present invention, rather than limitation of the invention further.
Embodiment 1
1) add 5 times of amounts of Semen Vitis viniferae, temperature is that 95 ℃ water circulates and extracts 2 times, each 2 hours, obtains extracting solution;
2) extracting solution is cooled to 38 ℃ and crosses macroporous resin;
3) with 60% ethanol elution macroporous resin;
4) with 3) after resulting eluent of step reclaims ethanol, under 70 ℃, concentrate, dry;
5) with 4) alcohol reflux of 5 times 95% of the dry thing reuse of step gained 1 time, each 2 hours;
6) after extracting solution reclaims ethanol, concentrated, dry under 70 ℃, promptly get product.
Embodiment 2
1) add 8 times of amounts of Semen Vitis viniferae, temperature is that 100 ℃ water circulates and extracts 3 times, each 3 hours, obtains extracting solution;
2) extracting solution is cooled to 40 ℃ and crosses macroporous resin;
3) with 80% ethanol elution macroporous resin;
4) with 3) after resulting eluent of step reclaims ethanol, under 90 ℃, concentrate, dry;
5) with 4) alcohol reflux of 8 times 97% of the dry thing reuse of step gained 3 times, each 3 hours;
6) after extracting solution reclaims ethanol, concentrated, dry under 90 ℃, promptly get product.
Embodiment 3
1) add 6 times of amounts of Semen Vitis viniferae, temperature is that 100 ℃ water circulates and extracts 2 times, each 2 hours, obtains extracting solution;
2) extracting solution is cooled to 35 ℃ and crosses macroporous resin;
3) with 70% ethanol elution macroporous resin;
4) with 3) after resulting eluent of step reclaims ethanol, under 80 ℃, concentrate, dry;
5) with 4) alcohol reflux 2 times of the 5-8 times of 90-97% of dry thing reuse of step gained, each 2 hours;
6) after extracting solution reclaims ethanol, concentrated, dry under 80 ℃, promptly get product.
Claims (5)
1, the preparation technology of Semen Vitis viniferae extract is characterized in that, technology may further comprise the steps:
1) add that Semen Vitis viniferae 5-8 doubly measures, temperature is that 95-100 ℃ water circulates and extracts 2-3 time, 2-3 hour at every turn, obtains extracting solution;
2) extracting solution is cooled to 20-40 ℃ and crosses macroporous resin;
3) the ethanol elution macroporous resin of usefulness 60-80%;
4) with 3) after resulting eluent of step reclaims ethanol, concentrate, dry;
5) with 4) alcohol reflux 1-3 time of the 5-8 times of 90-97% of dry thing reuse of step gained, 2-3 hour at every turn;
6) after extracting solution reclaims ethanol, concentrated, dry, promptly get product.
2, the preparation technology of Semen Vitis viniferae extract according to claim 1 is characterized in that, described 2) in the step with 70% ethanol elution.
3, the preparation technology of Semen Vitis viniferae extract according to claim 1 is characterized in that, of the present invention 4), 6) temperature of concentrate drying is 70-90 ℃ in the step.
4, the preparation technology of Semen Vitis viniferae extract according to claim 3 is characterized in that, concentrate drying be 80 ℃.
5, the preparation technology of Semen Vitis viniferae extract according to claim 1 is characterized in that, described 5) in the step dry thing with 6 times of amounts, 95% alcohol reflux 2 times, each 2 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810030763A CN101524446A (en) | 2008-03-07 | 2008-03-07 | Process for preparing grape seed extract |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810030763A CN101524446A (en) | 2008-03-07 | 2008-03-07 | Process for preparing grape seed extract |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101524446A true CN101524446A (en) | 2009-09-09 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810030763A Pending CN101524446A (en) | 2008-03-07 | 2008-03-07 | Process for preparing grape seed extract |
Country Status (1)
| Country | Link |
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| CN (1) | CN101524446A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101792428A (en) * | 2010-03-16 | 2010-08-04 | 浙江大学 | Drying method of grape seed proanthocyanidin extract |
| CN103435661A (en) * | 2013-08-13 | 2013-12-11 | 新疆西部牧业股份有限公司 | Extraction method for grape seed polyphenols |
| CN105669780A (en) * | 2016-01-14 | 2016-06-15 | 张敬明 | Method for raising polyphenol content and water-solubility of grape seed extract |
| CN105770213A (en) * | 2016-04-14 | 2016-07-20 | 天津药物研究院有限公司 | Grape-seed specific mean-degree-of-polymerization procyanidin extract and preparing method and application thereof |
| CN106806249A (en) * | 2016-11-01 | 2017-06-09 | 田鹏新 | A kind of skin cream containing grape pip ginkgo biloba p.e and preparation method thereof |
| CN113040392A (en) * | 2021-03-12 | 2021-06-29 | 广州市金龟寿药品有限公司 | Composite preparation containing ovalbumin peptide |
-
2008
- 2008-03-07 CN CN200810030763A patent/CN101524446A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101792428A (en) * | 2010-03-16 | 2010-08-04 | 浙江大学 | Drying method of grape seed proanthocyanidin extract |
| CN101792428B (en) * | 2010-03-16 | 2012-07-11 | 浙江大学 | Drying method of grape seed proanthocyanidin extract |
| CN103435661A (en) * | 2013-08-13 | 2013-12-11 | 新疆西部牧业股份有限公司 | Extraction method for grape seed polyphenols |
| CN105669780A (en) * | 2016-01-14 | 2016-06-15 | 张敬明 | Method for raising polyphenol content and water-solubility of grape seed extract |
| CN105770213A (en) * | 2016-04-14 | 2016-07-20 | 天津药物研究院有限公司 | Grape-seed specific mean-degree-of-polymerization procyanidin extract and preparing method and application thereof |
| CN106806249A (en) * | 2016-11-01 | 2017-06-09 | 田鹏新 | A kind of skin cream containing grape pip ginkgo biloba p.e and preparation method thereof |
| CN113040392A (en) * | 2021-03-12 | 2021-06-29 | 广州市金龟寿药品有限公司 | Composite preparation containing ovalbumin peptide |
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Open date: 20090909 |