CN102796067A - Method for extracting flavonoids from xanthoceras sorbifolia shells - Google Patents
Method for extracting flavonoids from xanthoceras sorbifolia shells Download PDFInfo
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- CN102796067A CN102796067A CN2012103211895A CN201210321189A CN102796067A CN 102796067 A CN102796067 A CN 102796067A CN 2012103211895 A CN2012103211895 A CN 2012103211895A CN 201210321189 A CN201210321189 A CN 201210321189A CN 102796067 A CN102796067 A CN 102796067A
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- shinyleaf yellowhorn
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- flavonoid substances
- yellowhorn shell
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Abstract
The invention aims to provide a method for extracting flavonoids from xanthoceras sorbifolia shells, which is high in efficiency, high in yield, short in time consumption, simple in operation, low in production cost and low in corrosivity to equipment. The technical scheme is that the method comprises the following steps: soaking the deoiled and crushed xanthoceras sorbifolia shells into a certain amount of solution; ultrasonically extracting in ultrasonic equipment for a certain time; performing suction-filtration on the extracting solution; and concentrating and freeze-drying the filtrate to obtain the xanthoceras sorbifolia flavonoid concentrate.
Description
Technical field
The present invention provides Flavonoid substances process for extracting in a kind of shinyleaf yellowhorn shell, belongs to technical field of agricultural product process.
Background technology
Wood of Shinyleaf Yellowhorn belongs to the Sapindaceae Wood of Shinyleaf Yellowhorn and belongs to, and also is referred to as civil official fruit, xanthoceras sobifolia bunge, Wen Guanhua etc., is the woody seeds of the distinctive preciousness of China.I measure the Wood of Shinyleaf Yellowhorn shell-containing rate and reach 53%, and flavones content reaches 7% in the shinyleaf yellowhorn shell.Flavonoid compound aboundresources and be easy to extract; In organs such as the stem of plant, leaf, flower, fruit, extensively exist; Be one group at structure and different in nature polyphenols, the platymiscium secondary metabolite, how the forms with free state or glycosides are stored in the plant vacuole.
Flavonoid compound adopts solvent extraction usually.Because of the structure of flavonoid compound is different with the source, dissolution characteristics difference is also very big.For the big slightly aglycon of flavonoid glycoside and polarity (like flavonol, bisflavone, look into youngster's ketone etc.), general used water, methyl alcohol, ethanol, acetone or bigger mixed solvent such as the methanol-water of polarity extract.Some polysaccharide glycosides can be used boiling water extraction.Most of Flavone aglycones then adopt chloroform, ether, the less solvent extraction of ETHYLE ACETATE isopolarity and traditional extraction process, though that technology is stablized is feasible, efficient is low, length consuming time, and the reagent consumption is big, and solvent is prone to residual, and environment is had certain infringement.Ultrasonic extraction is that mechanical effect, diffusion, heat effect and the cavatition of utilizing UW to produce are strengthened the release of intracellular matter, thereby quickens the diffusion and the leaching of effective components in plants.Hyperacoustic heat effect helps the dissolving of material, and therefore, it is short to have an extraction time, and effective component extraction rate is high, and cost is low, low-temperature protection thermo-sensitivity composition, pollute advantages such as little.
Reported the technology of having studied ultrasonic extraction method chrysanthemum cream colour ketone compounds in Teng Haipeng " total flavones in the ultrasonic-assisted extraction chrysanthemum rice ", final total flavones yield can reach 16.96%, has improved 6.59% than the yield of common extraction; " ultrasonic extraction Foliole banyan leaf flavonoids technical study " result of Wang Lisheng shows employing ultrasonic extraction method Foliole banyan leaf flavonoids; Extraction yield is 4.38%, compares with conventional hot extraction, and yield has improved 2.06%; Time has shortened 100min, and it is few to extract solvent load.Yet, for the research of ultrasonic-assisted extraction Wood of Shinyleaf Yellowhorn flavones, report is not arranged but at present.
Summary of the invention
The purpose of this invention is to provide and a kind ofly can overcome above-mentioned defective, and efficient, high, the consuming time weak point of yield, simple to operate, production cost is low, to Flavonoid substances process for extracting in the low shinyleaf yellowhorn shell of equipment corrosion.Its technical scheme is:
Flavonoid substances process for extracting in a kind of shinyleaf yellowhorn shell is characterized in that adopting following steps: (1) shinyleaf yellowhorn fruit shell is pulverized, and uses solution soaking 25～35 mins of the volume of 60%-70% as 10-25 times of milliliter of shinyleaf yellowhorn shell opaque amount gram number; (2) be 150～200 W at power, temperature is in 60～75 ℃ of ultrasonic equipments, ultrasonic extraction 25～50min; (3) extracting solution suction filtration is concentrated under 60～70 ℃ driedly, and freeze-drying gets Wood of Shinyleaf Yellowhorn flavones enriched material.
Flavonoid substances process for extracting in described a kind of shinyleaf yellowhorn shell, shinyleaf yellowhorn shell is pulverized order and is counted the 5-20 order in the step 1.
Flavonoid substances process for extracting in described a kind of shinyleaf yellowhorn shell, shinyleaf yellowhorn shell is that degreasing is deoiled in the step 1.
Flavonoid substances process for extracting in described a kind of shinyleaf yellowhorn shell adopts the water of condensation cycling extraction during ultrasonic extraction in the step 2.
Flavonoid substances process for extracting in described a kind of shinyleaf yellowhorn shell, ultrasonic extraction device in the step 2, used 20-60 KHz and 150-200 W.
Flavonoid substances process for extracting in described a kind of shinyleaf yellowhorn shell, the concentrating under reduced pressure temperature is 60～70 ℃ in the step 3, under 0.1～0.7 normal atmosphere.
The present invention compared with prior art, the technology that is adopted has only been used ethanol, and is almost harmless and cheap and easy to get to human body.Compare with traditional technology, technology is simple, extraction yield high (compare with simple ethanol extraction, the extraction yield increase has increased about 26%); Yield high (compare with simple ethanol extraction, yield has improved about 18%), amount of ethanol significantly reduces (under the identical extraction yield, than simple ethanol extraction; Amount of ethanol has reduced about 80%), production process is simple, and is easy to operate; Be prone to industriallization, mechanize, automatic production.
Preferred forms
Embodiment 1:
Step 1: using alcohol concn is 60%, and volume is that the ethanol extract dissolving 10g order number of 250mL is that 5 ~ 20 shinyleaf yellowhorn shell soaks 25 min;
Step 2: at power is 150W, and temperature is in 75 ℃ of ultrasonic equipments, ultrasonic extraction 35min;
Step 3: the extracting solution suction filtration, 0.1 normal atmosphere is concentrated under 65 ℃ driedly, and freeze-drying gets the Wood of Shinyleaf Yellowhorn flavones, and extraction yield is 86%.
Embodiment 2:
Step 1: using alcohol concn is 70%, and volume is that the ethanol extract dissolving 5g order number of 50 mL is that 5 ~ 20 shinyleaf yellowhorn shell soaks 35 min;
Step 2: at power is 150W, and temperature is in 75 ℃ of ultrasonic equipments, ultrasonic extraction 50min;
Step 3: the extracting solution suction filtration, 0.5 normal atmosphere is concentrated under 70 ℃ driedly, and freeze-drying gets the Wood of Shinyleaf Yellowhorn flavones, and extraction yield is 91%.Through Liebermann reaction and Liebermann-Burchard reaction, confirm to contain in the extract bunkankasaponin; Calculate through colorimetric method for determining, the saponin yield of two instances is respectively 8.4% and 6.9%.
Claims (7)
1. Flavonoid substances process for extracting in the shinyleaf yellowhorn shell, it is characterized in that adopting following steps: (1) shinyleaf yellowhorn fruit shell is pulverized, and the volume that uses 60%-70% is shinyleaf yellowhorn shell opaque amount gram number 10-30 solution soaking 25～35 min doubly milliliter; (2) be 150～200 W at power, temperature is in 60～75 ℃ of ultrasonic equipments, ultrasonic extraction 25～50 min; (3) extracting solution suction filtration is concentrated under 60～70 ℃ driedly, and freeze-drying gets Wood of Shinyleaf Yellowhorn flavones enriched material.
2. Flavonoid substances process for extracting in a kind of shinyleaf yellowhorn shell according to claim 1 is characterized in that: shinyleaf yellowhorn shell is pulverized order and is counted the 5-20 order.
3. Flavonoid substances process for extracting in a kind of shinyleaf yellowhorn shell according to claim 1 is characterized in that: used ethanol is that medical absolute ethyl alcohol adding distil water is deployed into.
4. Flavonoid substances process for extracting in a kind of shinyleaf yellowhorn shell according to claim 1 is characterized in that: used alcohol concn is between 60%-70%.
5. Flavonoid substances process for extracting in a kind of shinyleaf yellowhorn shell according to claim 1 is characterized in that: used ethanol volume milliliter number is 10-30 a times of shinyleaf yellowhorn shell quality gram number.
6. Flavonoid substances process for extracting in a kind of shinyleaf yellowhorn shell according to claim 1 is characterized in that: used ultrasonic frequency and power is 20-60 KHz and 150-200 W respectively.
7. Flavonoid substances process for extracting in a kind of shinyleaf yellowhorn shell according to claim 1 is characterized in that: used ultrasonic generator is the ultrasonic extraction device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2012103211895A CN102796067A (en) | 2012-09-04 | 2012-09-04 | Method for extracting flavonoids from xanthoceras sorbifolia shells |
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| CN2012103211895A CN102796067A (en) | 2012-09-04 | 2012-09-04 | Method for extracting flavonoids from xanthoceras sorbifolia shells |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105853475A (en) * | 2016-01-05 | 2016-08-17 | 浙江海洋学院 | Preparation method of pemphis acidula extract |
| CN106177101A (en) * | 2015-04-29 | 2016-12-07 | 甘肃长瑞文冠果产业发展有限公司 | The new application at middle mongolian medicine shinyleaf yellowhorn fruit shell position |
| CN113598368A (en) * | 2021-06-28 | 2021-11-05 | 长沙汉爵快乐养老服务有限公司 | Meal replacement powder assisting in reducing blood sugar and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20030096030A1 (en) * | 2001-10-31 | 2003-05-22 | Songjiang Wang | Extracting materials from the shell of Xanthoceras sorbifolia Bunge and applying the extracted materials to making drugs and functional foods |
| US20040146591A1 (en) * | 2001-08-31 | 2004-07-29 | Yun Wang | Composition comprising wenguanguo extracts, methods for preparing same and uses thereof |
| CN101242850A (en) * | 2003-10-09 | 2008-08-13 | 太平洋艾瑞有限公司 | Composition, function and use of xanthoceras sorbifolia extract and compound isolated from same, method for preparing same |
| CN101416669A (en) * | 2007-10-26 | 2009-04-29 | 陕西文冠果业科技开发有限公司 | A kind of tealeaves that contains the bunkankasaponin bioactivator |
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2012
- 2012-09-04 CN CN2012103211895A patent/CN102796067A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US20040146591A1 (en) * | 2001-08-31 | 2004-07-29 | Yun Wang | Composition comprising wenguanguo extracts, methods for preparing same and uses thereof |
| US20030096030A1 (en) * | 2001-10-31 | 2003-05-22 | Songjiang Wang | Extracting materials from the shell of Xanthoceras sorbifolia Bunge and applying the extracted materials to making drugs and functional foods |
| CN101242850A (en) * | 2003-10-09 | 2008-08-13 | 太平洋艾瑞有限公司 | Composition, function and use of xanthoceras sorbifolia extract and compound isolated from same, method for preparing same |
| CN101416669A (en) * | 2007-10-26 | 2009-04-29 | 陕西文冠果业科技开发有限公司 | A kind of tealeaves that contains the bunkankasaponin bioactivator |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106177101A (en) * | 2015-04-29 | 2016-12-07 | 甘肃长瑞文冠果产业发展有限公司 | The new application at middle mongolian medicine shinyleaf yellowhorn fruit shell position |
| CN105853475A (en) * | 2016-01-05 | 2016-08-17 | 浙江海洋学院 | Preparation method of pemphis acidula extract |
| CN113598368A (en) * | 2021-06-28 | 2021-11-05 | 长沙汉爵快乐养老服务有限公司 | Meal replacement powder assisting in reducing blood sugar and preparation method thereof |
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Application publication date: 20121128 |