CN101513170A - Breeding method for high-oleic acid high-yield peanut - Google Patents
Breeding method for high-oleic acid high-yield peanut Download PDFInfo
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Abstract
The invention provides a breeding method for high-oleic acid high-yield peanut, which includes steps: (1) coordinate parent combination: selecting high-yield germplasm with good integrated character and germplasm with high oleic acid content SPI098 to combine patent; (2) high oleic acid pressure screening: determining oleic acid of each seed in excellent single plant in early separation generation, and selecting excellent peanut seed with high oleic acid content; (3) high-yield pressure screening: planting the screened peanut seed with high oleic acid content in next generation, separating generation in anaphase, and selecting excellent high-yield single plant; and (4) high-oleic acid high-yield peanut variety or strain obtaining: obtaining novel high-oleic acid high-yield peanut variety or strain through multi-generation high-oleic acid high-yield pressure screening. The breeding method provided by the present invention directly screens according to character of oleic acid content in peanut seed, and has advantages of short breeding period, small blindness, high efficiency, and good economic benefit and social benefit.
Description
Technical field
The present invention relates to a breeding method of cultivating peanut, relate in particular to a kind of breeding method of high-oleic acid high-yield peanut.
Background technology
Peanut is main oil crop of China and economic crops, and main fatty acid is oleic acid and linoleic acid in the peanut, and linoleic acid is the basic reason that causes grease putrid and deteriorated.In the common peanut, the content of oleic acid is about 45%, and linoleic content is about 35%.With the common peanut is that the peanut oil that raw material makes contains a large amount of linoleic acid, if adding preservative agent not is easy to putrid and deteriorated; If the interpolation preservative then has a negative impact to health.In recent ten years, the linoleic acid content of the oleic acid content of raising peanut, reduction peanut is the research focus that the peanut breeding worker improves peanut quality always.Oleic acid can reduce the amount of blood total cholesterol and bad cholesterol, but does not reduce the amount of useful cholesterol.Nutrition circle calls oleic acid " safe fatty acid " usually, and the content of oleic acid is one of important symbol of evaluation edible oil quality.Therefore, the new peanut variety of cultivation high-oleic acid high-yield has important economic implications and social effect.
In the prior art, utilize the sexual hybridization breeding technique that peanut is carried out genetic improvement, improving output, enhancing disease resistance and aspect such as adaptability has obtained immense success.But, but to make slow progress aspect the peanut high-oleic acid breed improvement, effect is very limited.Cause the main cause of this situation to be: the breeder can select by the quality trait of oleic acid content in segregative generation is directly selected proterties such as the output of peanut, disease resistances and can't be to peanut, promptly can't carry out directive breeding according to the quality trait of oleic acid content in the peanut.Therefore, the quality trait of oleic acid content is difficult to improve by the sexual hybridization breeding in the peanut.At present, be that the breeding technique of purpose only limits to the high oleic acid parental combination of assembly and according to correlation filial generation selected indirectly with oleic acid content in the improvement peanut.Because high oleic acid parent material seldom, and can't directly select high oleic acid proterties, therefore, the high preproinsulin gene in the colony can not be fixed and add up and run off, cause to be difficult to obtain good high-oleic acid high-yield peanut kind or the strain of comprehensive proterties, and breeding efficiency is low.This patent first inventor's Yu Shanlin in 1993 through cobalt
60The mutant that gamma rays irradiation 79266 strains obtain, through continuous 6 generations selfing obtain, new lines peanut SPI098 stable, high oleic acid content, oleic acid content reaches 85.95%, is the highest erect type peanut new lines of present oleic acid content, and just output is lower.
Summary of the invention
The breeding method that the purpose of this invention is to provide a kind of high-oleic acid high-yield peanut, this method have breeding method simple, can be according to the advantage that the proterties of oleic acid content in the peanut is directly screened, breeding cycle is short, blindness is little, efficient is high.
Technical scheme of the present invention
Technical scheme of the present invention is achieved in that the breeding method of high-oleic acid high-yield peanut provided by the invention, may further comprise the steps:
(1) assembly parental combination: select that comprehensive proterties is good, the germplasm SPI098 assembly parental combination of the germplasm of high yield and high oleic acid content;
(2) high oleic acid pressure screening: because oleic acid content is by key-gene control (fourth brocade equality, the genetic analysis of arachic acid linoleic acid ratio (O/L), Chinese oil crop journal, 2007,29 (3): 233～237).Therefore, segregative generation is measured the oleic acid content of each seed in the fine individual plant in early days, selects the good peanut seed of high oleic acid content;
(3) high yield pressure screening: peanut seed that filter out, high oleic acid content is planted from generation to generation at next, at the later stage segregative generation, selects the high yield fine individual plant;
(4) obtain high-oleic acid high-yield peanut kind or strain: carry out directive breeding and obtain high-oleic acid high-yield peanut kind or strain through many high oleic acid and screenings of high yield pressure from generation to generation.
Described comprehensive proterties germplasm good, high yield is meant higher yield traits, stronger disease resistance and adaptability, and the shape of plant type, branch amount, pod and size reach the requirement of production.
The present invention can also realize with following preferential technical scheme:
In the step (1), the germplasm SPI098 of described high oleic acid content, be this patent first inventor's Yu Shanlin in 1993 through cobalt
60The mutant that gamma rays irradiation 79266 strains obtain, through continuous 6 generations selfing obtain, new lines peanut stable, high oleic acid content, its oleic acid content reaches 85.95%.Described 79266 strains are formed by Shandong Peanut Inst.'s seed selection, this strain combines proterties such as early-maturing and large, high-quality, salt tolerant alkali, wide adaptability together, belong to precocious upright seedling major peanut, can be in Shandong and the plantation of northern major peanut producing region, be fit to spring sowing, cornfield interplanting and summer sowing.
In the step (2), the mensuration of described oleic acid content is to adopt nondestructive method, utilizes near infrared spectrometer to measure the fatty acid component and measures oleic acid content in the peanut single seed.(certificate number: introduced the method for this nondestructive mensuration peanut single seed oleic acid content 2007-J-201-2.), the present invention utilizes the method to measure the oleic acid content of each peanut seed in the fine individual plant to the national science and technology progress second prize " special-purpose new peanut variety initiative technical research and application " that this patent first applicant obtained in 2007.
In the step (2), described early stage segregative generation is F2～F3 generation.
In step (1), (2) or (3), described high oleic acid content is meant that oleic acid content is higher than 75%.
In the step (3), described plantation is the plantation of individual plant simple grain, and the directed screening of its offspring colony is the individual plant screening.
In the step (3), described later stage segregative generation is F4～F6 generation.
In the step (4), described many generations are 4～6 generations.
Beneficial effect of the present invention
The breeding method of high-oleic acid high-yield peanut provided by the invention, have breeding method simple, according to the proterties of oleic acid content in the peanut seed is directly screened, breeding cycle is short, blindness is little, efficient is high characteristics.The mensuration of oleic acid content adopts nondestructive analytical technology in the peanut single seed, and this technology is simple to operate, accuracy is high, finding speed is fast, can satisfy the requirement of breeding.The breeding method of high-oleic acid high-yield peanut provided by the invention, utilization is carried out high oleic acid pressure screening and the screening of high yield pressure respectively at different segregative generations, high oleic acid and two kinds of merits of high yield are combined together, cultivate oleic acid content height, comprehensive good new peanut variety or the strain of proterties, have good economic benefits and social benefit.
Embodiment
Further specify technical scheme of the present invention below by specific embodiment, the present invention includes but be not limited to following embodiment.To the change that does not break away from flesh and blood of the present invention that the present invention carried out, still belong to protection scope of the present invention according to prior art.
Embodiment 1
Strain S17 with anti-aphid, two spotted spider mite in 2000 (from the international semiarid institute of India) carries out hybrid combination with the germplasm SPI098 collocation of high oleic acid content, and adds generation winter in Hainan.The hybrid peanut field trial method for designing of breeding routinely carries out the parental combination arrangement, and the individual plant simple grain is planted in the field, and F1 does not carry out any selection from generation to generation.At F2 still is from generation to generation that unit carries out the plantation of individual plant simple grain with the parental combination, and the hybrid generation at F2 strong separation takes place from generation to generation.From 30 seeds of fine individual plant of screening, adopt non-destructive method to measure the single seeded oleic acid content of peanut, therefrom filter out 4 oleic acid contents and be higher than 75% single seed.Selected high oleic acid content seed continues the plantation of individual plant simple grain from generation to generation at F3, obtains 82 seeds altogether, adopts non-destructive method to measure single seeded oleic acid content, and wherein oleic acid content is higher than 75% seed 31.Segregative generation screens according to the proterties of oleic acid content in the peanut seed in early days, and wherein oleic acid content is higher than 75% seed and goes into to elect as elite seed, is called high oleic acid pressure screening.The single seed of selected high oleic acid content is proceeded the plantation of individual plant simple grain from generation to generation at F4, through the screening of high yield pressure, therefrom filters out a strain fine individual plant.At the later stage segregative generation, select the high yield fine individual plant, be called the screening of high yield pressure.Carry out directive breeding through many high oleic acid pressure screening and screenings of high yield pressure from generation to generation, obtains stable, comprehensively the good high-oleic acid high-yield peanut kind flower of proterties is educated No. 32, is the product 1 of inventing.
Flower is educated No. 32: 120 days breeding times.Plant type is upright, plant height 36.04cm, the long 39.4cm of side shoot, 8 of total branch amounts, 12 of per plant, individual plant productivity 21g.The common shape of pod, the several 775/kg of fruit, the several 1602/kg of benevolence, the heavy 173.0g of all kinds of fruits, the heavy 67g of hundred benevolence, milled rice rate 71.3%, protein content 26.3%, fat content 50.7%, oleic acid content 77.8%, linoleic acid content 6.3%, O/L value (oleic acid and linoleic ratio also claim oil inferior ratio) 12.3.It is neat to emerge, and plant is upright, and branch is few, and growth is sane.
Regional test
2006 and 2007, the regional test that invention product 1 (flower is educated No. 32) are participated in Shandong Province's new peanut variety, reference substance 1 spends 12 for the Shandong, and the result is as shown in table 1:
The regional test result of table 1. invention product 1 and reference substance 1
As can be seen from Table 1: 2006 and 2007, the average pod output of invention product 1 was respectively 283.7kg/ mu and 264.11kg/ mu, increases production 4.1% and 5.03% respectively than reference substance 1; The average seed benevolence output of invention product 1 is respectively 205.4kg/ mu and 187.55kg/ mu, increases production 3.1% and 4.92% respectively than reference substance 1.
Production test
2008, invention product 1 (flower is educated No. 32) were participated in Shandong Province's new peanut variety production test, and reference substance 1 spends 12 for the Shandong, and the result is as shown in table 2:
The mill trial results of table 2. invention product 1 and reference substance 1
As can be seen from Table 2: the average pod output of invention product 1 and average seed benevolence output are respectively 286.13kg/ mu and 211.4kg/ mu, increase production 11.25% and 10.91% respectively than reference substance 1.
Invention product 1 (flower is educated No. 32) passed through Shandong Province's crop varietal approval committee in 2009.
Embodiment 2
Make male parent with safe 8603 (seed selections of Taizhou, Jiangsu Province institute of agricultural sciences), carry out hybrid combination with SPI098 as female parent, each selected from generation to generation peanut seed carries out the plantation of individual plant simple grain.F1 does not select from generation to generation; At F2～F3 from generation to generation, carry out high oleic acid pressure screening.Selected oleic acid content surpasses 75% single seed in F4～F5 generation, through the screening of high yield pressure, therefrom filters out a strain fine individual plant.Select and directive breeding through many high oleic acid and high yield pressure duals from generation to generation, obtain stable, the comprehensive good high-oleic acid high-yield peanut kind E16 of proterties, i.e. invention product 2.
E16: about 114 days breeding times.Dredge the branch type, stem is high 37.5 centimetres, long 42.6 centimetres of side shoot, 5～7 of total branches, about 5 of fruit bearing shoot, several 12.5 of per plant, the strain clump is short and upright, compactness, internode is short, and resistant to lodging, the leaf look dark green, blooms continuously, the amount of blooming is big, the ripening rate height, and two a kind of fruit, such as apple, pear, etc. rates generally account for more than 95%, carpopodium is short, is difficult for shedding, the pod plain edition, all kinds of fruits weigh 171.5 grams, and hundred benevolence weigh 67.1 grams, milled rice rate 72.5%.Oleic acid content 78.6%, O/L value (oleic acid and linoleic ratio also claim oil inferior ratio) 12.8.
Variety comparative test
Invention product 2 (E16) carry out variety comparative test in Shandong Peanut Inst., and reference substance 2 is educated No. 20 for flower, and the result is as shown in table 3:
The variety comparative test result of table 3. invention product 2 and reference substance 2
As can be seen from Table 3: invention product 2 average pod output 224.76kg/ mus, seed benevolence output 164.21kg/ mu, increase production 5.18% and 7.71% respectively than reference substance 2.
Embodiment 3
Spend 14 (seed selections of Henan Province province Puyang Academy of Agricultural Sciences) to make male parent with Pu, carry out hybrid combination with SPI098 as female parent, each selected from generation to generation seed all carries out the plantation of individual plant simple grain.F1 does not screen from generation to generation; Carry out high oleic acid pressure screening from generation to generation at F2～F3, selected oleic acid content carries out the screening of high yield pressure above 75% high oily single seed from generation to generation at F4～F6, therefrom filters out a strain fine individual plant.Through the high oleic acid of many generations and the screening and the directive breeding of high yield pressure, obtain stable, the comprehensive good high-oleic acid high-yield peanut kind E12 of proterties, i.e. invention product 3.
E12: dredge the upright small pod peanut of branch type, it is neat to emerge, and growth is sane, and Grain Dormancy, drought resistance are strong, than disease-resistanting leaf spot and net blotch.125 days breeding times.Stem is high 35.4 centimetres, long 41.3 centimetres of side shoot, and all kinds of fruits weigh 151.6 grams, and hundred benevolence weigh 63.8 grams, milled rice rate 72.1%; Crude fat content 53.5%, protein content 23.2%, oleic acid content 81.5%, O/L value (oleic acid and linoleic ratio also claim oil inferior ratio) 21.5.
Variety comparative test
Invention product 3 (E12) carry out variety comparative test in Shandong Peanut Inst., and reference substance 2 is educated No. 20 for flower, and the result is as shown in table 4:
The variety comparative test result of table 4. invention product 3 and reference substance 2
As can be seen from Table 4: invention product 3 average pod output 281.5kg/ mus, seed benevolence output 211.7kg/ mu, increase production 5.5% and 4.8% respectively than reference substance 2 (flower is educated No. 20).
Claims (9)
1. the breeding method of a high-oleic acid high-yield peanut is characterized in that: may further comprise the steps:
(1) assembly parental combination: select that comprehensive proterties is good, the germplasm SPI098 assembly parental combination of the germplasm of high yield and high oleic acid content;
(2) high oleic acid pressure screening: segregative generation is measured the oleic acid content of each seed in the fine individual plant in early days, selects the good peanut seed of high oleic acid content;
(3) high yield pressure screening: peanut seed that filter out, high oleic acid content is planted from generation to generation at next, at the later stage segregative generation, selects the high yield fine individual plant;
(4) obtain high-oleic acid high-yield peanut kind or strain: carry out directive breeding and obtain high-oleic acid high-yield peanut new varieties or strain through many high oleic acid and screenings of high yield pressure from generation to generation.
2. the breeding method of high-oleic acid high-yield peanut according to claim 1 is characterized in that: the germplasm SPI098 of described high oleic acid content, be this patent first inventor's Yu Shanlin in 1993 through cobalt
60The mutant that gamma rays irradiation 79266 strains obtain, through continuous 6 generations selfing obtain, new lines peanut stable, high oleic acid content, its oleic acid content reaches 85.95%.
3. the breeding method of high-oleic acid high-yield peanut according to claim 1, it is characterized in that: the mensuration of described oleic acid content adopts nondestructive method, utilizes near infrared spectrometer to measure the fatty acid component and measures oleic acid content in the peanut single seed.
4. the breeding method of high-oleic acid high-yield peanut according to claim 1 is characterized in that: described early stage segregative generation be F2～F3 from generation to generation.
5. the breeding method of high-oleic acid high-yield peanut according to claim 1 is characterized in that: described high oleic acid pressure screening is to screen according to the proterties of oleic acid content in the peanut seed.
6. the breeding method of high-oleic acid high-yield peanut according to claim 1, it is characterized in that: described high oleic acid content is meant that oleic acid content is higher than 75%.
7. the breeding method of high-oleic acid high-yield peanut according to claim 1 is characterized in that: described plantation is the plantation of individual plant simple grain, and the directed screening of its offspring colony is the individual plant screening.
8. the breeding method of high-oleic acid high-yield peanut according to claim 1 is characterized in that: described later stage segregative generation be F4～F6 from generation to generation.
9. the breeding method of high-oleic acid high-yield peanut according to claim 1 is characterized in that: be 4～6 generations from generation to generation more than described.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101880594A (en) * | 2010-06-06 | 2010-11-10 | 山东省花生研究所 | Preparation method of high oleic acid virgin peanut oil |
| CN102283100A (en) * | 2011-07-06 | 2011-12-21 | 山东省花生研究所 | Method for breeding high-oleic-acid disease-resistance peanuts |
| CN102283101A (en) * | 2011-07-07 | 2011-12-21 | 山东省花生研究所 | High-oleic-acid, drought-resistant and barren-resistant peanut variety breeding method |
| CN104255433A (en) * | 2014-09-09 | 2015-01-07 | 河南省农业科学院 | Development and authentication method for allohexaploid peanuts |
| CN104542264A (en) * | 2015-01-14 | 2015-04-29 | 山东省花生研究所 | Breeding method of high-oleic-acid dormancy peanut variety |
| CN107593431A (en) * | 2017-10-26 | 2018-01-19 | 山东省花生研究所 | A kind of continuous breeding method of type high oleic acid peanut varieties of crawling of yielding positive results |
| CN107969338A (en) * | 2017-12-05 | 2018-05-01 | 山东省花生研究所 | A kind of method for creating the high oily new germ plasm of peanut seed |
| CN108812295A (en) * | 2018-06-06 | 2018-11-16 | 山东省花生研究所 | A kind of high oleic acid salt tolerant peanut selection |
| CN111109073A (en) * | 2020-01-03 | 2020-05-08 | 吉林省农业科学院 | Echelon selective character breeding method for peanuts |
| CN113455376A (en) * | 2021-06-03 | 2021-10-01 | 开封市农林科学研究院 | Screening method of high-oleic-acid peanut backbone parent |
| CN114766352A (en) * | 2022-04-02 | 2022-07-22 | 山东省花生研究所 | Method for cultivating excellent characters of peanuts |
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2009
- 2009-03-23 CN CN2009101284847A patent/CN101513170B/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101880594A (en) * | 2010-06-06 | 2010-11-10 | 山东省花生研究所 | Preparation method of high oleic acid virgin peanut oil |
| CN102283100A (en) * | 2011-07-06 | 2011-12-21 | 山东省花生研究所 | Method for breeding high-oleic-acid disease-resistance peanuts |
| CN102283101A (en) * | 2011-07-07 | 2011-12-21 | 山东省花生研究所 | High-oleic-acid, drought-resistant and barren-resistant peanut variety breeding method |
| CN104255433A (en) * | 2014-09-09 | 2015-01-07 | 河南省农业科学院 | Development and authentication method for allohexaploid peanuts |
| CN104255433B (en) * | 2014-09-09 | 2016-08-31 | 河南省农业科学院 | The initiative of a kind of allohexaploid Semen arachidis hypogaeae and authentication method |
| CN104542264A (en) * | 2015-01-14 | 2015-04-29 | 山东省花生研究所 | Breeding method of high-oleic-acid dormancy peanut variety |
| CN107593431A (en) * | 2017-10-26 | 2018-01-19 | 山东省花生研究所 | A kind of continuous breeding method of type high oleic acid peanut varieties of crawling of yielding positive results |
| CN107969338A (en) * | 2017-12-05 | 2018-05-01 | 山东省花生研究所 | A kind of method for creating the high oily new germ plasm of peanut seed |
| CN108812295A (en) * | 2018-06-06 | 2018-11-16 | 山东省花生研究所 | A kind of high oleic acid salt tolerant peanut selection |
| CN111109073A (en) * | 2020-01-03 | 2020-05-08 | 吉林省农业科学院 | Echelon selective character breeding method for peanuts |
| CN113455376A (en) * | 2021-06-03 | 2021-10-01 | 开封市农林科学研究院 | Screening method of high-oleic-acid peanut backbone parent |
| CN114766352A (en) * | 2022-04-02 | 2022-07-22 | 山东省花生研究所 | Method for cultivating excellent characters of peanuts |
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