CN106797884A - A kind of selection of high oleic acid Silk channel injection - Google Patents
A kind of selection of high oleic acid Silk channel injection Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
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Abstract
The present invention relates to peanut breeding technical field, and in particular to a kind of selection of high oleic acid Silk channel injection.Selection of the invention, comprises the following steps：(1) again by by for selfing, directed screening, obtaining high oleic acid strain 8 153 of the high oleic acid content not less than 80% after maternal 1 is hybridized with male parent 1；(2) again by by for selfing, directed screening, obtaining high oleic acid strain totally 11 plant of the high oleic acid content not less than 80% after high oleic acid strain 8 153 is hybridized with another male parent 2；(3) again by by for selfing, directed screening, obtaining high oleic acid peanut new germ plasm after 11 plant height oleic acid peanut strains are hybridized with another male parent 3 respectively；The peanut new germ plasm that is selected using this breeding technique at the same have that oleic acid content is high, linoleic acid content is low, high yield, kernel percent higher, resistance to bacterial wilt, disease-resistanting leaf spot, Resistant Gene To Rust the characteristics of.
Description
【Technical field】
The present invention relates to peanut breeding technical field, and in particular to a kind of selection of high oleic acid Silk channel injection.
【Background technology】
Peanut is the main oil crops and industrial crops of China, in peanut main aliphatic acid be oleic acid and linoleic acid,
In common peanut, the content of oleic acid is about 45%, and linoleic content is about 35%.Wherein, linoleic acid is to cause grease corrupt
Rotten basic reason, and oleic acid can reduce blood of human body T-CHOL and harmful LDL-C, but to having
The HDL-C of benefit does not influence then, significant in terms of the heart disease risks such as coronary heart disease are reduced；Separately
Outward, oleic acid be difficult to be oxidized, product shelf-stable, it may have important industrial economy value.The vegetable oil of high oleic acid content is in city
It is widely loved by consumers on field, such as have an olive oil of " liquid golden " good reputation, oleic acid content is up to more than 80%, also camellia
Oil, oleic acid content is also very high, and nutrition circle generally calls oleic acid " safe fats acid ", and the content of oleic acid is the edible oil product of evaluation
One of important symbol of matter.In recent ten years, the oleic acid content for improving peanut, the linoleic acid content for reducing peanut are always peanut
Breeder improves the study hotspot of peanut quality.Further, since peanut is the topmost industrial crops of China and oil plant making
One of thing, high yield is always the main target of peanut breeding.
Peanut high-oleic acid breeding research is just carried out early in the eighties in 20th century in the U.S..1987, Norden etc. was from right
Oleic acid content 80% or so high oleic acid germplasm " F435 " (Norden et are found that in the fatty acid analysis of peanut breeding material
al,1987).With " F435 " for high oleic acid parent, Florida, US agricultural experiment station is existed by sexual hybridization, backcrossing means
Nineteen ninety-five has been bred as high oleic acid peanut varieties " Sunoleic95R " (Gorbet and Knauft 1997) earliest.Subsequent
In 10 years or so times, it is new that U.S.'s breeding units or company have been bred as more than 10 high oleic acid peanuts such as " SunOliec97 " in succession again
Kind (Chen Jing, 2011).After domestic peanut high-oleic acid breeding research probably starts from 2000, including Guangxi Academy of Agricultural Sciences's economy
Domestic some research institutions in where crop investigations introduce high oleic acid Silk channel injection from the U.S. in succession carries out research on utilization.2003
Year Yu Shanlin etc. obtains a collection of high oleic acid mutant using method of radiating, using " SP1098 " therein for parent educated in 2009
Into high oleic acid new varieties " flower educates 32 " and by Shandong Province's crop varietal approval committee.Kaifeng academy of agricultural sciences in
It is bred as within 2006 high oleic acid kind " opening agriculture H03-3 " and by Anhui Province's cultivar identification (Chen Jing, 2011).Cut-off 2015, I
State has been bred as 18 high oleic acid new peanut varieties (Wang Chuantang, 2015 in succession；Wang Chuantang, 2016).But in south China peanut
Producing region, so far, the incubation report still without high oleic acid peanut varieties.It is local that southern ecoclimatic condition is mainly suitable for plantation
The Spanish type kind of seed selection, the peanut varieties quoted from northern China or foreign countries are inclined in local plantation common manifestation result
Less, yield is unstable, later stage early ageing, disease resistance difference etc., it is difficult to directly applied in production.Guangxi Academy of Agricultural Sciences's warp in 2001
Ji crop research institute introduces high oleic acid kind " Sunoleic95R " from the U.S., finds that it there is also after planting experimentally above-mentioned similar
Problem, thus decision breeding improvement is localized to it, by with mixing breed in region, select and be adapted in south China
The Pearl Type high oleic acid peanut new germ plasm of peanut producing region plantation.
【The content of the invention】
Goal of the invention of the invention is：Produced present in local planting experimentally for high oleic acid kind " Sunoleic95R "
Measure low and other proterties defects, select the plantation of suitable one's respective area and with high oleic acid, low linoleic acid and high yield, kernel percent compared with
Height, the strong Pearl Type high oleic acid peanut new germ plasm of field Resistance to bacterial wilt, leaf spot resistance, rust resistance.
To achieve these goals, the technical solution adopted by the present invention is as follows：
A kind of selection of high oleic acid Silk channel injection, the described method comprises the following steps：
(1) maternal 1 is hybridized with male parent 1, is obtained first generation filial generation F1, the seed of F1 generation is rejected into the mixed receipts of pseudostationary
After carry out selfing and obtain F2 generations, F3 generations will be obtained after the seed selfing in F2 generations, F4 will be obtained for seed after the seed selfing in F3 generations；
Oleic acid content analysis is carried out for benevolence to the F4, and filters out Kernel of Peanut of the oleic acid content not less than 80%, obtain oil high
Sour peanut strain 8-153；
(2) the high oleic acid peanut strain 8-153 of step (1) with the male parent 2 of another high-yield disease resisting hybridize and obtain another
One filial generation F ' 1, F ' 2 generation seeds are obtained by selfing is carried out after the generations of the F ' 1 rejecting mixed receipts of pseudostationary, and oil is carried out to the F ' 2 generation benevolences
Acid content is analyzed, and filters out high oleic acid peanut strain totally 11 plant of the oleic acid content not less than 80%；The 11 plant height oleic acid flower
Raw strain is respectively：High oleic acid strain 14, high oleic acid strain 16, high oleic acid strain 107, high oleic acid strain 109, high oleic acid strain
150th, high oleic acid strain 212, high oleic acid strain 421, high oleic acid strain 425, high oleic acid strain 586, high oleic acid strain 902 and height
Oleic acid strain 1083；
(3) 11 plant height oleic acid peanut strains of step (2) are hybridized with the male parent 3 of another high-yield disease resisting respectively
Filial generation F " 1, " selfing was carried out and obtains F " 2 generations by F after the seed mixed receipts of rejecting pseudostationary in 1 generation, and by F, " selfing that carries out in 2 generations is obtained
In the generations of F " 3, in F " 2 generations and F " 3 generations, are carried out into mixed planting and obtain F " 4 generations, by F " 4 generations carried out selfing and obtain F " 5 after rejecting pseudostationary
Generation；F " 5 generations carried out selfing and obtain F " 6 generations；To the F, " 6 generation benevolences carry out oleic acid content and analyze and carry out strain comparing, selection
Elite plant strain enters identification garden, 2 years yield and quality qualification tests of multiple spot is then carried out, so as to obtain high oleic acid peanut novel species
Matter.
Further, described maternal 1 is Shanyou 162, and the male parent 1 is Sunleic95R, and the male parent 2 is Guangdong oil 13.
Further, the male parent 3 is profound oil 75.
Further, the male parent 3 is Guangdong oil 45.
Further, oleic acid content analysis is carried out to F4 generations and/or F ' 2 generation benevolences in step (1) and/or step (2)
Method is high resolution gas chromatography method.
Further, to F in step (3), " method that 6 generation benevolences carry out oleic acid content analysis is near-infrared analysis method.
Above-mentioned parent material source：
" Sunleic95R " is the height oil introduced from the U.S. in 2001 by industrial crops research institute of Guangxi Academy of Agricultural Sciences
Sour kind (oleic acid content 80% or so) (Gorbet and Knauft 1997)；
" Shanyou 162 " is to be bred as (Zheng Yixiong, 2005) by Shantou City, Guangdong Province Institute of agricultural sciences；
" Guangdong oil 13 " and " Guangdong oil 45 " is to be bred as (Hong Yanbin, 2011 by Crop Institute, Guangdong Academy of Agricultural Sciences；Lee is few
Hero, 2010)；
" profound oil 75 " is to be bred as (Wang Yun, 2011) by Zhanjiang, Guangdong Province Institute of agricultural sciences.
The beneficial effects of the invention are as follows：
1st, the present invention is carried out using local high yield, the peanut varieties of disease resistance high and the kind " Sunleic95R " introduced
Sexual hybridization, in crossover process, in addition to the germplasm materials of M8003 line high-quality, is also entered by 2 sexual hybridizations to filial generation
Row improvement, further increases the genetic recombination ability of peanut, realizes the complementation of merit, effectively increases and realizes breeding mesh
Target success rate, further shortens breeding time, while so that the new germ plasm for selecting is suitable for south China ecology gas
Time condition, reaches that oleic acid content is high, linoleic acid content is low and high yield, and kernel percent is higher, and field Resistance to bacterial wilt, leaf spot resist
Property, rust resistance is strong and advantage of the plantation of suitable one's respective area.
2nd, in breeding process, the present invention analyzes peanut high-oil acid content using nondestructive NIR technology,
The technical operation is simple, the degree of accuracy is high, finding speed is fast, can meet the requirement of breeding.
【Brief description of the drawings】
Fig. 1 is the family tree of breeding method of the present invention.
【Specific embodiment】
With reference to embodiment, the invention will be further described.
Embodiment 1：
A kind of selection of high oleic acid Silk channel injection is as shown in figure 1, the method is comprised the following steps：
(1) 2003 year spring, by maternal 1 (Shanyou 162：The characteristics of with high-yield disease resisting) (SunOliec95R is with male parent 1
The high oleic acid peanut varieties that applicant is introduced by 948 projects from the U.S., its oil is analyzed through oil analysis test center of the Ministry of Agriculture
Acid content 64.14%, linoleic acid 15.86%, oil/Asia are compared and 4.04) are hybridized, and obtain first generation filial generation F1, and the spring in 2004 will
Selfing being carried out after the seed mixed receipts of rejecting pseudostationary of F1 generation and obtaining F2 generations, F2 generations all individual plants when harvesting individually are harvested；2004
Autumn in year will obtain F3 generations after the seed selfing in F2 generations, the spring in 2005 will obtain F4 for seed after the seed selfing in F3 generations；To described
F4 carries out oleic acid content analysis for benevolence, and (analysis method is：High resolution gas chromatography method), and filter out oleic acid content and be not less than
80% Kernel of Peanut, and acquisition high oleic acid peanut strain 8-153 (compared with former high oleic acid parent " Sunleic95R ", " 8-153 "
Plant shape become upright, pod also becomes big and becomes many, but its yield also big gap compared with local main breed)；
(2) 2006 year autumn, by (the Guangdong oil of male parent 2 of the high oleic acid peanut strain 8-153 of step (1) and another high-yield disease resisting
13：The characteristics of with high-yield disease resisting) hybridize and obtain another filial generation F ' spring in 1,2007, the generations of F ' 1 are rejected pseudostationary is mixed to receive
After carry out selfing and obtain F ' 2 generation seeds, F ' 2 withholds all individual plants when obtaining and individually harvests；Oleic acid is carried out to the F ' 2 generation benevolences to contain
(analysis method is for amount analysis：High resolution gas chromatography method), and Kernel of Peanut of the oleic acid content not less than 80% is filtered out, obtain 11
Plant height oleic acid peanut strain；The 11 plant height oleic acid peanut strain is respectively：High oleic acid strain 14, high oleic acid strain 16, oil high
Sour strain 107, high oleic acid strain 109, high oleic acid strain 150, high oleic acid strain 212, high oleic acid strain 421, high oleic acid strain
425th, high oleic acid strain 586, high oleic acid strain 902 and high oleic acid strain 1083 are (in 2008-2009, to the height of above-mentioned acquisition
Oleic acid peanut strain carries out strain and variety test of lines, finds the yield traits of these high oleic acid peanut strains or not enough manages
Think, pod is few and universal shorter)；
(3) 2010 spring, (the present embodiment was only explanation of giving one example, above-mentioned by the high oleic acid peanut strain 425 of step (2)
11 plant height oleic acid peanut strains：High oleic acid strain 14, high oleic acid strain 16, high oleic acid strain 107, high oleic acid strain 109, height
Oleic acid strain 150, high oleic acid strain 212, high oleic acid strain 421, high oleic acid strain 425, the strain of high oleic acid strain 586, high oleic acid
Be that 902 and high oleic acid strain 1083 can meet requirement of the invention) with the male parent 3 of another high-yield disease resisting (profound oil 75：With height
The characteristics of producing disease-resistant) obtain filial generation F " 1, F " was carried out into selfing and obtains F " 2 generations after the seed mixed receipts of rejecting pseudostationary in 1 generation, by F " 2
The selfing that carries out in generation obtains F, and " in F " 2 generations and F " 3 generations, are carried out mixed planting and obtain F " 4 generations, by F " 4 generations after rejecting pseudostationary by 3 generations
Carry out selfing and obtain F " 5 generations；In F " 5 generations carried out selfing and obtain F " 6 generations, (in the Breeding Process in F " 1 generation-F " 6 generations, eliminate result
Then the relatively poor individual plant of relatively fewer, economical character is planted again)；To the F, " 6 generation benevolences carry out oleic acid content point
(analysis method is for analysis：Near-infrared analysis method) and strain comparing is carried out, selection elite plant strain enters identification garden, then carries out 2 years
The yield and quality qualification test of multiple spot, obtains high oleic acid peanut new germ plasm 1 (12 autumn strain/5-3).
Embodiment 2
A kind of selection of high oleic acid Silk channel injection is as shown in figure 1, the method is comprised the following steps：
(1) 2003 year spring, by maternal 1 (Shanyou 162：The characteristics of with high-yield disease resisting) (SunOliec95R is with male parent 1
The high oleic acid peanut varieties that applicant is introduced by 948 projects from the U.S., its oil is analyzed through oil analysis test center of the Ministry of Agriculture
Acid content 64.14%, linoleic acid 15.86%, oil/Asia are compared and 4.04) are hybridized, and obtain first generation filial generation F1, and the spring in 2004 will
Selfing being carried out after the seed mixed receipts of rejecting pseudostationary of F1 generation and obtaining F2 generations, F2 generations all individual plants when harvesting individually are harvested；2004
Autumn in year will obtain F3 generations after the seed selfing in F2 generations, the spring in 2005 will obtain F4 for seed after the seed selfing in F3 generations；To described
F4 carries out oleic acid content analysis for benevolence, and (analysis method is：High resolution gas chromatography method), and filter out oleic acid content and be not less than
80% Kernel of Peanut, and acquisition high oleic acid peanut strain 8-153 (compared with former high oleic acid parent " Sunleic95R ", " 8-153 "
Plant shape become uprightly, pod also become big and become many, but its yield also big gap compared with local main breed)；
(2) 2006 year autumn, by (the Guangdong oil of male parent 2 of the high oleic acid peanut strain 8-153 of step (1) and another high-yield disease resisting
13：The characteristics of with high-yield disease resisting) hybridize and obtain another filial generation F ' spring in 1,2007, the generations of F ' 1 are rejected pseudostationary is mixed to receive
After carry out selfing and obtain F ' 2 generation seeds, F ' 2 withholds all individual plants when obtaining and individually harvests；Oleic acid is carried out to the F ' 2 generation benevolences to contain
(analysis method is for amount analysis：High resolution gas chromatography method), and Kernel of Peanut of the oleic acid content not less than 80% is filtered out, obtain 11
Plant height oleic acid peanut strain；The 11 plant height oleic acid peanut strain is respectively：High oleic acid strain 14, high oleic acid strain 16, oil high
Sour strain 107, high oleic acid strain 109, high oleic acid strain 150, high oleic acid strain 212, high oleic acid strain 421, high oleic acid strain
425th, high oleic acid strain 586, high oleic acid strain 902 and high oleic acid strain 1083 are (in 2008-2009, to the height of above-mentioned acquisition
Oleic acid peanut strain carries out strain and variety test of lines, finds the yield traits of these high oleic acid peanut strains or not enough manages
Think, pod is few and universal shorter)；
(3) 2010 spring, (the present embodiment was only explanation of giving one example, above-mentioned by the high oleic acid peanut strain 16 of step (2)
11 plant height oleic acid peanut strains：High oleic acid strain 14, high oleic acid strain 16, high oleic acid strain 107, high oleic acid strain 109, height
Oleic acid strain 150, high oleic acid strain 212, high oleic acid strain 421, high oleic acid strain 425, the strain of high oleic acid strain 586, high oleic acid
Be that 902 and high oleic acid strain 1083 can meet requirement of the invention) with (the Guangdong oil 45 of another male parent 3：Spy with high-yield disease resisting
Point) obtain filial generation F " 1, F " was carried out into selfing and obtains F " 2 generations after the seed mixed receipts of rejecting pseudostationary in 1 generation, by F, " the carrying out in 2 generations is certainly
Friendship obtains F " F " 2 generations with F " 3 generations are carried out mixed planting and obtain F by 3 generations " 4 generations, rejects after pseudostationary that " 4 generations carried out selfing and obtain by F
" 5 generations to F；F " 5 generations carried out selfing and obtain F " 6 generations (in the Breeding Process in F " 1 generation-F " 6 generations, eliminate result it is relatively fewer,
Then the relatively poor individual plant of economical character is planted again)；To the F, " 6 generation benevolences carry out oleic acid content analysis (analysis side
Method is：Near-infrared analysis method) and strain comparing is carried out, selection elite plant strain enters identification garden, then carries out the 2 years yield of multiple spot
Quality Identification is tested, and obtains high oleic acid peanut new germ plasm 2 (12 autumn strain/1-3) and new germ plasm 3 (12 autumn strain/1-8).
Above-mentioned new germ plasm 2 has following characteristic after measured：
In about 125 days or so the time of infertility, plant type is uprightly compact, and growth potential is strong, in blade, leaf color green.Each economical character
And economic characters species test average value is：Stem 60.8cm high, long branch 62.1cm, total branch amount 6.5,4.7, fruitful branch are single
Strain number of results 13.3, plumpness 87.17%, double a kind of fruit, such as apple, pear, etc. rates 79.3%, all kinds of fruits weight 186.4g, hundred benevolence weight 63.6g, kilogram fruit number
668, kernel percent 60.22%.
Plant experimentally contrast test：
Contrast test 1：
Experiment year and place：2013；Nanning Wuming County.
Test specimen：The new germ plasm 2 (12 obtained in the new germ plasm 1 (12 autumn strain/5-3), the embodiment 2 that are obtained in embodiment 1
Autumn strain/1-3) and new germ plasm 3 (12 autumn strain/1-8).
Control sample：Shanyou 523.
Cultural technique：Selection is sunny, and soil layer is deep, is test block rich in organic matter and calcareous sandy loam, presses
Planted experimentally according to unified standard and field management.Plantation is planted using " playing furrow duplicate rows double grains " mode.Specification：Furrow are wide 80 centimetres
(33 centimetres of row ditch, furrow face are wide 47 centimeters), furrow are high 13-16 centimetres, per the row of drill seeding kind two, two 23-27 centimetres of line-spacings, hill spacing 13-
16 centimetres, every bunch planting kind 2.Every mu of sowing each sample Kernel of Peanut is equal in weight.
Field management：Carried out by unified standard, heavy dressing base manure topdresses in good time.Mu basal dressing：Phosphate fertilizer (45 kilograms)+peanut
Bran (20 kilograms)+pigs and cattle excrement (130 kilograms)+plant ash (75) kilogram+farmyard manure (4000 kilograms), after blended stack retting becomes thoroughly decomposed,
65% spreads fertilizer over the fields and makees base fertilizer, and 35% makees lid seed manure.After planting timely herbicide spraying.During 3-4 piece true leaves, fitted according to seedling growing way and applied
6.5 kilograms of urea.Pin ridging is met after blooming 15 days, calcium, potash fertilizer (25 kilograms of 25 kilograms+plant ash of lime) is imposed.
Interpretation of result：Contained by the oleic acid in Ministry of Agriculture's oil plant and quality of item supervision and inspection center analysis test seed benevolence
Amount, counts and calculates per mu yield, and concrete outcome see the table below 1.
The result of the test statistical form of table 1
As can be seen that the oleic acid content of new germ plasm 1, new germ plasm 2 and new germ plasm 3 is all apparently higher than right from the result of upper table 1
According to kind, the Pod Yield of new germ plasm 2 is more slightly higher than control kind, and new germ plasm 1, the Pod Yield of new germ plasm 3 plant slightly lower than control.
In order to further carry out stability and universality experiment to new germ plasm 2, the present invention is further tried
Test research：
Contrast test 2：
Experiment year：2014 and 2015.
Test site：Guangxi region：Including Nanning, Guilin, the North Sea, He Prefecture, Liuzhou, Pingnan County and other places；National south
Square piece：Including Fujian Nanan, gaozhou,guangdong and other places.
Test specimen：The new germ plasm 2 (12 autumn strain/1-3) obtained in embodiment 2.
Control sample：Shanyou 523 and sweet osmanthus 21.
Cultural technique：Selection is sunny, and soil layer is deep, is test block rich in organic matter and calcareous sandy loam, presses
Planted experimentally according to unified standard and field management.Plantation is planted using " playing furrow duplicate rows double grains " mode.Specification：Furrow are wide 80 centimetres
(33 centimetres of row ditch, furrow face are wide 47 centimeters), furrow are high 13-16 centimetres, per the row of drill seeding kind two, two 23-27 centimetres of line-spacings, hill spacing 13-
16 centimetres, every bunch planting kind 2.Every mu of sowing each sample Kernel of Peanut is equal in weight.
Field management：Carried out by unified standard, heavy dressing base manure topdresses in good time.Mu basal dressing：Phosphate fertilizer (45 kilograms)+peanut
Bran (20 kilograms)+pigs and cattle excrement (130 kilograms)+plant ash (75) kilogram+farmyard manure (4000 kilograms), after blended stack retting becomes thoroughly decomposed,
65% spreads fertilizer over the fields and makees base fertilizer, and 35% makees lid seed manure.After planting timely herbicide spraying.During 3-4 piece true leaves, fitted according to seedling growing way and applied
6.5 kilograms of urea.Pin ridging is met after blooming 15 days, calcium, potash fertilizer (25 kilograms of 25 kilograms+plant ash of lime) is imposed.
Interpretation of result：
(1) the average crude fat of seed benevolence of new germ plasm 2 is determined by Ministry of Agriculture's oil plant and quality of item supervision and inspection center
Content；And the average oleic acid content in fatty acid constituents, average linoleic acid content and the sub- ratio of average oil are determined, concrete outcome sees below
Table 2.
(2) according to national south piece and Guangxi peanut variety regional Disease Resistance Identification, the sick nursery of new germ plasm 2 and artificial
Inoculated identification disease resistance, concrete outcome see the table below 3.
(3) count and calculate the average pod production of new germ plasm 2, concrete outcome see the table below 4.
The average crude fat of the new germ plasm 2 of table 2, oleic acid, linoleic acid content and oil/Asia ratio
From upper table 2, the average oleic acid content of new germ plasm 2 is planted apparently higher than control；Average linoleic acid content is substantially low
In control kind；Averagely oil is sub- than being planted apparently higher than control.
The disease resistance test table of the new germ plasm 2 of table 3
From upper table 3, the Resistance to bacterial wilt of new germ plasm 2, leaf spot resistance and rust resistance are superior to control kind.
The average pod production statistical form of the new germ plasm 2 of table 4
From upper table 4, the average pod production of new germ plasm 2 is suitable with control kind.
In sum, peanut breeding is carried out using the method for the present invention, high oleic acid kind can be effectively improved
The defects such as what " Sunoleic95R " existed when Guangxi is planted yield poorly, disease-resistant difference, can select suitable one's respective area plantation
And with high oleic acid, low linoleic acid and high yield, kernel percent is higher, field Resistance to bacterial wilt, leaf spot resistance, rust resistance are equal
Strong Pearl Type high oleic acid peanut new germ plasm.
Described above is directed to the detailed description of preferably possible embodiments of the invention, but embodiment is not limited to this hair
Bright patent claim.
Claims (6)
1. a kind of selection of high oleic acid Silk channel injection, it is characterised in that the described method comprises the following steps：
(1) maternal 1 is hybridized with male parent 1, is obtained first generation filial generation F1, it is laggard that the seed of F1 generation is rejected into the mixed receipts of pseudostationary
Row selfing obtains F2 generations, will obtain F3 generations after the seed selfing in F2 generations, will obtain F4 for seed after the seed selfing in F3 generations；To institute
State F4 carries out oleic acid content analysis for benevolence, and filters out Kernel of Peanut of the oleic acid content not less than 80%, obtains high oleic acid flower
Raw strain 8-153；
(2) the high oleic acid peanut strain 8-153 of step (1) with the male parent 2 of another high-yield disease resisting hybridize and obtain another son
For F ' 1, F ' 2 generation seeds are obtained by selfing is carried out after the generations of the F ' 1 rejecting mixed receipts of pseudostationary, carrying out oleic acid to the F ' 2 generation benevolences contains
Amount analysis, and filter out high oleic acid peanut strain totally 11 plant of the oleic acid content not less than 80%；The 11 plant height oleic acid peanut strain
System is respectively：High oleic acid strain 14, high oleic acid strain 16, high oleic acid strain 107, high oleic acid strain 109, high oleic acid strain 150,
High oleic acid strain 212, high oleic acid strain 421, high oleic acid strain 425, high oleic acid strain 586, high oleic acid strain 902 and high oleic acid
Strain 1083；
(3) 11 plant height oleic acid peanut strains of step (2) with the male parent 3 of another high-yield disease resisting hybridize respectively and obtain another
Filial generation F " 1, " selfing was carried out and obtains F " 2 generations by F after the seed mixed receipts of rejecting pseudostationary in 1 generation, and by F, " selfing that carries out in 2 generations is obtained
In the generations of F " 3, in F " 2 generations and F " 3 generations, are carried out into mixed planting and obtain F " 4 generations, by F " 4 generations carried out selfing and obtain F " 5 after rejecting pseudostationary
Generation；F " 5 generations carried out selfing and obtain F " 6 generations；To the F, " 6 generation benevolences carry out oleic acid content and analyze and carry out strain comparing, selection
Elite plant strain enters identification garden, 2 years yield and quality qualification tests of multiple spot is then carried out, so as to obtain high oleic acid peanut novel species
Matter.
2. the selection of a kind of high oleic acid Silk channel injection according to claim 1, it is characterised in that described maternal 1 is
Shanyou 162, the male parent 1 is Sunleic95R, and the male parent 2 is Guangdong oil 13.
3. the selection of a kind of high oleic acid Silk channel injection according to claim 1, it is characterised in that the male parent 3 is
Profound oil 75.
4. the selection of a kind of high oleic acid Silk channel injection according to claim 1, it is characterised in that the male parent 3 is
Guangdong oil 45.
5. the selection of a kind of high oleic acid Silk channel injection according to claim 1, it is characterised in that step (1) and/or
In step (2) to F4 generation and/or F ' 2 generation benevolences carry out oleic acid content analysis method be high resolution gas chromatography method.
6. the selection of a kind of high oleic acid Silk channel injection according to claim 1, it is characterised in that right in step (3)
The method that F " 6 generation benevolences carry out oleic acid content analysis is near-infrared analysis method.
Priority Applications (1)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089419A (en) * | 2019-05-21 | 2019-08-06 | 广东省农业科学院作物研究所 | A kind of breeding method of high-oleic acid high-yield peanut |
| CN110367116A (en) * | 2019-06-17 | 2019-10-25 | 广东省农业科学院作物研究所 | A kind of breeding method of high oleic acid peanut hybridization |
| CN111316909A (en) * | 2019-03-25 | 2020-06-23 | 河南益人花生科技研究院 | Breeding method of high-oleic-acid drought-tolerant peanuts |
| CN114451294A (en) * | 2022-02-22 | 2022-05-10 | 濮阳市农业科学院 | Breeding method of high-oleic-acid high-yield peanuts |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105532444A (en) * | 2015-12-11 | 2016-05-04 | 广西壮族自治区农业科学院经济作物研究所 | Breeding method for novel peanut variety |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105532444A (en) * | 2015-12-11 | 2016-05-04 | 广西壮族自治区农业科学院经济作物研究所 | Breeding method for novel peanut variety |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111316909A (en) * | 2019-03-25 | 2020-06-23 | 河南益人花生科技研究院 | Breeding method of high-oleic-acid drought-tolerant peanuts |
| CN111316909B (en) * | 2019-03-25 | 2023-04-28 | 河南益人花生科技研究院 | Breeding method of high-oleic acid drought-resistant peanuts |
| CN110089419A (en) * | 2019-05-21 | 2019-08-06 | 广东省农业科学院作物研究所 | A kind of breeding method of high-oleic acid high-yield peanut |
| CN110367116A (en) * | 2019-06-17 | 2019-10-25 | 广东省农业科学院作物研究所 | A kind of breeding method of high oleic acid peanut hybridization |
| CN110367116B (en) * | 2019-06-17 | 2022-03-11 | 广东省农业科学院作物研究所 | High-oleic acid peanut hybridization breeding method |
| CN114451294A (en) * | 2022-02-22 | 2022-05-10 | 濮阳市农业科学院 | Breeding method of high-oleic-acid high-yield peanuts |
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