CN101507740A - 利用大孔树脂制备细梗香草总皂苷提取物的方法及用途 - Google Patents
利用大孔树脂制备细梗香草总皂苷提取物的方法及用途 Download PDFInfo
- Publication number
- CN101507740A CN101507740A CNA2009100967860A CN200910096786A CN101507740A CN 101507740 A CN101507740 A CN 101507740A CN A2009100967860 A CNA2009100967860 A CN A2009100967860A CN 200910096786 A CN200910096786 A CN 200910096786A CN 101507740 A CN101507740 A CN 101507740A
- Authority
- CN
- China
- Prior art keywords
- herba lysimachiae
- total saponin
- macroporous resin
- lysimachiae capillipedis
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007949 saponins Chemical class 0.000 title claims abstract description 55
- 239000011347 resin Substances 0.000 title claims abstract description 54
- 229920005989 resin Polymers 0.000 title claims abstract description 54
- 229930182490 saponin Natural products 0.000 title claims abstract description 54
- 239000001397 quillaja saponaria molina bark Substances 0.000 title claims abstract description 44
- 239000000284 extract Substances 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title claims description 4
- 241000941637 Lysimachia capillipes Species 0.000 title abstract 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 66
- 238000010828 elution Methods 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 17
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 15
- 238000000605 extraction Methods 0.000 claims abstract description 11
- 238000010438 heat treatment Methods 0.000 claims abstract description 9
- 238000010992 reflux Methods 0.000 claims abstract description 9
- 239000003814 drug Substances 0.000 claims abstract description 7
- 238000001914 filtration Methods 0.000 claims abstract description 7
- 238000001694 spray drying Methods 0.000 claims abstract description 7
- 235000017709 saponins Nutrition 0.000 claims description 47
- 239000007788 liquid Substances 0.000 claims description 23
- 238000010298 pulverizing process Methods 0.000 claims description 5
- 230000006837 decompression Effects 0.000 claims description 4
- 239000004793 Polystyrene Substances 0.000 claims description 3
- 239000002253 acid Substances 0.000 claims description 3
- 230000001476 alcoholic effect Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 229920002223 polystyrene Polymers 0.000 claims description 3
- 230000000259 anti-tumor effect Effects 0.000 abstract description 6
- 229940079593 drug Drugs 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000003480 eluent Substances 0.000 abstract 3
- 238000005119 centrifugation Methods 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000003795 desorption Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 17
- 238000002474 experimental method Methods 0.000 description 9
- 230000002401 inhibitory effect Effects 0.000 description 7
- 241000699670 Mus sp. Species 0.000 description 6
- BMWPBKOFJSHJAW-UHFFFAOYSA-N Saponin B Natural products CC1(C)CCC2(CCC3(C)C(=CCC4C5(C)CCC(OC6OC(CO)C(O)C(OC7OC(CO)C(O)C(O)C7O)C6=O)C(C)(C)C5CCC34C)C2C1)C(=O)O BMWPBKOFJSHJAW-UHFFFAOYSA-N 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 208000029742 colonic neoplasm Diseases 0.000 description 3
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- MYRTYDVEIRVNKP-UHFFFAOYSA-N 1,2-Divinylbenzene Chemical compound C=CC1=CC=CC=C1C=C MYRTYDVEIRVNKP-UHFFFAOYSA-N 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 2
- 208000026310 Breast neoplasm Diseases 0.000 description 2
- 206010009944 Colon cancer Diseases 0.000 description 2
- 206010058467 Lung neoplasm malignant Diseases 0.000 description 2
- 206010033128 Ovarian cancer Diseases 0.000 description 2
- 206010061535 Ovarian neoplasm Diseases 0.000 description 2
- 241000208476 Primulaceae Species 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- 210000000683 abdominal cavity Anatomy 0.000 description 2
- 201000008275 breast carcinoma Diseases 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000012531 culture fluid Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 201000005202 lung cancer Diseases 0.000 description 2
- 208000020816 lung neoplasm Diseases 0.000 description 2
- 239000012567 medical material Substances 0.000 description 2
- 238000007920 subcutaneous administration Methods 0.000 description 2
- 210000004881 tumor cell Anatomy 0.000 description 2
- 230000005760 tumorsuppression Effects 0.000 description 2
- INLFWQCRAJUDCR-IQVMEADQSA-N (1R,2S,4S,5'S,6R,7S,8R,9S,12S,13S)-5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.02,9.04,8.013,18]icosane-6,2'-oxane] Chemical compound O([C@@H]1[C@@H]([C@]2(CC[C@@H]3[C@@]4(C)CCCCC4CC[C@H]3[C@@H]2C1)C)[C@@H]1C)[C@]11CC[C@H](C)CO1 INLFWQCRAJUDCR-IQVMEADQSA-N 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- 206010008342 Cervix carcinoma Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 208000000461 Esophageal Neoplasms Diseases 0.000 description 1
- 208000005016 Intestinal Neoplasms Diseases 0.000 description 1
- 241000612166 Lysimachia Species 0.000 description 1
- 208000019255 Menstrual disease Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
- 206010030155 Oesophageal carcinoma Diseases 0.000 description 1
- 206010039491 Sarcoma Diseases 0.000 description 1
- 208000007271 Substance Withdrawal Syndrome Diseases 0.000 description 1
- 241000053227 Themus Species 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 239000003463 adsorbent Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 208000006673 asthma Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 201000010881 cervical cancer Diseases 0.000 description 1
- 201000010897 colon adenocarcinoma Diseases 0.000 description 1
- 230000000112 colonic effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000013872 defecation Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- WQLVFSAGQJTQCK-UHFFFAOYSA-N diosgenin Natural products CC1C(C2(CCC3C4(C)CCC(O)CC4=CCC3C2C2)C)C2OC11CCC(C)CO1 WQLVFSAGQJTQCK-UHFFFAOYSA-N 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 201000004101 esophageal cancer Diseases 0.000 description 1
- 206010016766 flatulence Diseases 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 206010017758 gastric cancer Diseases 0.000 description 1
- 208000010749 gastric carcinoma Diseases 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 150000002338 glycosides Chemical class 0.000 description 1
- 210000003701 histiocyte Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 201000002313 intestinal cancer Diseases 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000003361 porogen Substances 0.000 description 1
- FVSKHRXBFJPNKK-UHFFFAOYSA-N propionitrile Chemical compound CCC#N FVSKHRXBFJPNKK-UHFFFAOYSA-N 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- NWMIYTWHUDFRPL-UHFFFAOYSA-N sapogenin Natural products COC(=O)C1(CO)C(O)CCC2(C)C1CCC3(C)C2CC=C4C5C(C)(O)C(C)CCC5(CCC34C)C(=O)O NWMIYTWHUDFRPL-UHFFFAOYSA-N 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000012798 spherical particle Substances 0.000 description 1
- 238000012109 statistical procedure Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 201000000498 stomach carcinoma Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 150000003538 tetroses Chemical class 0.000 description 1
- 150000004043 trisaccharides Chemical class 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- -1 α-Jia Jibenyixi Chemical compound 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009100967860A CN101507740B (zh) | 2009-03-19 | 2009-03-19 | 利用大孔树脂制备细梗香草总皂苷提取物的方法及用途 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009100967860A CN101507740B (zh) | 2009-03-19 | 2009-03-19 | 利用大孔树脂制备细梗香草总皂苷提取物的方法及用途 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN101507740A true CN101507740A (zh) | 2009-08-19 |
CN101507740B CN101507740B (zh) | 2011-10-26 |
Family
ID=41000377
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2009100967860A Active CN101507740B (zh) | 2009-03-19 | 2009-03-19 | 利用大孔树脂制备细梗香草总皂苷提取物的方法及用途 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN101507740B (zh) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102180938A (zh) * | 2011-03-30 | 2011-09-14 | 浙江大学 | 细梗香草皂苷的制备方法 |
CN103330791A (zh) * | 2013-06-05 | 2013-10-02 | 虞天龙 | 一种以细梗香草为主体的抗癌中草药配方 |
CN104817601A (zh) * | 2015-03-31 | 2015-08-05 | 浙江大学 | 从细梗香草中提取三个黄酮类成分混合体、制剂及其方法 |
CN105037483A (zh) * | 2015-08-18 | 2015-11-11 | 苏州求是本草健康科技有限公司 | 一种细梗香草皂苷a的制备方法及其用途 |
CN105596388A (zh) * | 2016-02-15 | 2016-05-25 | 湖南城市学院 | 一种细梗香草口服液配方及制备工艺 |
CN105726359A (zh) * | 2016-02-14 | 2016-07-06 | 湖南城市学院 | 一种防癌护肤品及其制备方法 |
CN113061156A (zh) * | 2021-03-25 | 2021-07-02 | 浙江大学 | 一种细梗香草皂苷a的制备方法 |
CN114264763A (zh) * | 2021-12-22 | 2022-04-01 | 金绿源(中国)生物科技有限公司 | 一种细梗香草的取样方法 |
-
2009
- 2009-03-19 CN CN2009100967860A patent/CN101507740B/zh active Active
Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102180938A (zh) * | 2011-03-30 | 2011-09-14 | 浙江大学 | 细梗香草皂苷的制备方法 |
CN103330791A (zh) * | 2013-06-05 | 2013-10-02 | 虞天龙 | 一种以细梗香草为主体的抗癌中草药配方 |
CN104817601A (zh) * | 2015-03-31 | 2015-08-05 | 浙江大学 | 从细梗香草中提取三个黄酮类成分混合体、制剂及其方法 |
CN104817601B (zh) * | 2015-03-31 | 2018-04-10 | 浙江大学 | 从细梗香草中提取三个黄酮类成分混合体、制剂及其方法 |
CN105037483A (zh) * | 2015-08-18 | 2015-11-11 | 苏州求是本草健康科技有限公司 | 一种细梗香草皂苷a的制备方法及其用途 |
CN105726359A (zh) * | 2016-02-14 | 2016-07-06 | 湖南城市学院 | 一种防癌护肤品及其制备方法 |
CN105596388A (zh) * | 2016-02-15 | 2016-05-25 | 湖南城市学院 | 一种细梗香草口服液配方及制备工艺 |
CN113061156A (zh) * | 2021-03-25 | 2021-07-02 | 浙江大学 | 一种细梗香草皂苷a的制备方法 |
CN113061156B (zh) * | 2021-03-25 | 2022-04-19 | 浙江大学 | 一种细梗香草皂苷a的制备方法 |
CN114264763A (zh) * | 2021-12-22 | 2022-04-01 | 金绿源(中国)生物科技有限公司 | 一种细梗香草的取样方法 |
Also Published As
Publication number | Publication date |
---|---|
CN101507740B (zh) | 2011-10-26 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101507740B (zh) | 利用大孔树脂制备细梗香草总皂苷提取物的方法及用途 | |
CN102600219B (zh) | 黄蜀葵花总黄酮提取物及其制备方法 | |
CN103058978B (zh) | 从镰形棘豆中同步制备乔松素和2’,4’-二羟基查尔酮的工艺方法 | |
CN101904974B (zh) | 一种治疗恶性肿瘤疾病的中药组合物及其制备方法 | |
CN102134268B (zh) | 竹节参皂苷Ⅳa制备方法以及在制备保肝降酶药物中的应用 | |
CN101654485A (zh) | 一种用于治疗恶性肿瘤的白花蛇舌草多糖及其制备方法 | |
CN100475228C (zh) | 一种杏香兔耳风提取物及其制备方法 | |
CN104031156A (zh) | 夏枯草多糖锌螯合物及其制备方法与在抗癌药物中的应用 | |
CN105832789A (zh) | 一种利用高压微波辅助-半仿生法提取制备皂苷富集物的方法 | |
CN101537036A (zh) | 一种皂荚皂苷提取物及其制备方法与应用 | |
CN1775267A (zh) | 一种开口箭提取物及其制备方法和应用 | |
CN104873570A (zh) | 一种夏枯草总黄酮的提取纯化方法及其应用 | |
CN101912425A (zh) | 具有抗肿瘤及免疫促进活性的银杏外种皮提取物及其有效部位的制备方法 | |
CN104857245A (zh) | 紫萼玉簪花总皂苷的制备方法和应用 | |
CN101632747B (zh) | 小儿感冒舒颗粒的制备工艺 | |
CN102138957B (zh) | 药西瓜提取物及其生产方法和应用 | |
CN101618069B (zh) | 一种兼治瘀肿、出血和镇痛的胶囊制剂,其制备方法及用途 | |
CN108186773B (zh) | 一种减肥降脂药物制剂及其制备方法 | |
CN1864725A (zh) | 虎眼万年青活性提取物的制备方法及用途 | |
CN103169844B (zh) | 具有抗肺癌和肝癌作用的中药组合物 | |
CN103263491A (zh) | 中药组合物 | |
CN102362993B (zh) | 一种具有护肠清毒功效的中药制剂及其制备方法 | |
CN101073611B (zh) | 一种抗肿瘤的药物组合物 | |
CN109078115A (zh) | 一种用于消化道出血的中药制剂及其制备方法 | |
CN103393738B (zh) | 一种防治甲型h1n1流感病毒的中药组合物 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right |
Effective date of registration: 20220119 Address after: 215500 first floor, Changshu Cooperative Research Institute, Zhejiang University, No. 1-2, research institute road, Changshu Economic and Technological Development Zone, Suzhou, Jiangsu Province Patentee after: CHANGSHU QIUSHI TECHNOLOGY Co.,Ltd. Address before: 310027 No. 38, Zhejiang Road, Hangzhou, Zhejiang, Xihu District Patentee before: ZHEJIANG University |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240205 Address after: Room 1013, Building 1, No. 502 Linping Avenue, Hangzhou Yuhang Economic Development Zone, Linping District, Hangzhou City, Zhejiang Province, 311119 Patentee after: Hangzhou Yirui Pharmaceutical Technology Co.,Ltd. Country or region after: China Address before: 215500 first floor, Changshu Cooperative Research Institute, Zhejiang University, No. 1-2, research institute road, Changshu Economic and Technological Development Zone, Suzhou, Jiangsu Province Patentee before: CHANGSHU QIUSHI TECHNOLOGY Co.,Ltd. Country or region before: China |
|
TR01 | Transfer of patent right |