CN101486612A - Method producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation method - Google Patents
Method producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation method Download PDFInfo
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- CN101486612A CN101486612A CNA2009100147185A CN200910014718A CN101486612A CN 101486612 A CN101486612 A CN 101486612A CN A2009100147185 A CNA2009100147185 A CN A2009100147185A CN 200910014718 A CN200910014718 A CN 200910014718A CN 101486612 A CN101486612 A CN 101486612A
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Abstract
The invention provides a method for producing a seaweed fertilizer by processing Enteromorpha through microorganism fermentation method, which is characterized in that: fresh Enteromorpha is used as the material without being added with any chemical products, and treated by fermentation in large scale by preparing an Enteromorpha seed liquid, so as to dissolve out the effective components of Enteromorpha, thereby largely preserving the effective components with natural activity in the Enteromorpha, and then various Enteromorpha seaweed fertilizers are prepared by taking the fermentation liquid as a basic liquid. The method has simple extraction technique and low cost, and can process the Enteromorpha in large scale in a short time; the various Enteromorpha seaweed fertilizers produced by taking the fermentation liquid as the material can greatly increase the coldness resistance, drought resistance and stress resistance of crops, reduce plant diseases and insect pests, promote the growing of root system, make the soil to breathe freely and loosen, and greatly improve the crop output and quality, more importantly, the seaweed fertilizer does not produe any bad effect and pollution to crops, environments and human beings, which is a novel green environmental friendly fertilizer for modern agriculture.
Description
Technical field
The present invention relates to biodegradation technique and fertilizer production technology field, is a kind of method of producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation specifically.
Background technology
The Enteromorpha that break out in marine site, 6-7 month Qingdao in 2008, people are called " green evil spirit ", are a kind of great green pollution in the marine site, Qingdao.Qingdao the army and the people Great War Enteromorpha of struggling jointly, the Enteromorpha of thousands of tons comes out from salvage, because moisture content reaches 85%-90%, moreover temperature is higher, the Enteromorpha of disembarkation is easy to rancid and becomes pollution, has a strong impact on the urban environment in Qingdao.How can fast Enteromorpha be disposed, to alleviate The Surroundings in Cities is polluted, and Enteromorpha is effectively utilized, this is the problem that the present invention faces.
Summary of the invention
The objective of the invention is the problem that exists at prior art, a kind of method of producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation is provided, handle fresh Enteromorpha fast, on a large scale, technology is simple, mild condition, do not destroy effective constituent in the marine alga.And utilize fermenting enteromorpha liquid producting proportion rationally, use conveniently, cost is low, fertilizer efficiency is high various Enteromorpha marine alga fertilizers.
The objective of the invention is to be achieved through the following technical solutions: a kind of method of producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation, it is characterized in that, comprising:
The preparation of A, Enteromorpha seed liquor
Microbial strains is through the composite bacteria after screening and the domestication, is made up of bacterium, actinomycetes and fungi, all adapts to the higher environment of salinity in the fermenting enteromorpha; Sugar, protein, Mierocrystalline cellulose and organism all there is very strong capacity of decomposition;
Bacterium, actinomycetes, fungi are carried out submerged fermentation respectively to be cultivated, fermented liquid is formed according to bacterium: actinomycetes: fungi=1:1:1 mixes, in mixed fermentation liquid, add the necessary nutrition source of Enteromorpha biological degradation simultaneously as the biodegradable seed liquor of Enteromorpha, nutrition source is according to mixed fermentation liquid weight percent: nitrogen 0.2%-5%, phosphorus 2.5%-10%, potassium 1%-6% and micro-0.3-5%;
B, large scale fermentation are handled Enteromorpha
In fresh Enteromorpha, add the Enteromorpha biological degradation seed liquor of 1%-5% according to fresh Enteromorpha gross weight, cover by plastic film then and the mud sealing, cause the anaerobic environment of fermenting enteromorpha, utilize anaerobic bacterium in the bacterial classification Enteromorpha of degrading, whole anaerobic treatment process need time 20-30 days.Wherein, 50-60 ℃ pliotherm period 1-10 days, 35-50 ℃ 3-10 days middle temperature phase, 28-35 ℃ hypothermic phase 10-15 days; After anaerobically fermenting finishes, open the sealing on top, carry out 3-15 days facultative aerobic fermentation, this process is utilized actinomycetes and the fungi in the bacterial classification, to further decompose less than the Enteromorpha that degraded is good in anaerobic fermentation process, and can remove foul smell in the anaerobic fermentation process, the effective constituent in the Enteromorpha is discharged fully, then fermenting enteromorpha liquid is filtered, as all kinds of seaweed fertilizer of feedstock production.
C, the preparation of Enteromorpha fertilizer
With fermenting enteromorpha liquid is at least a compatibility in base fluid and humic acids, urea, potassium primary phosphate, the trace element, mixes, and has just made seaweed fertilizer.
To improvement of the technical scheme:
Each composition weight proportioning is: fermenting enteromorpha liquid: 10%-80%, and humic acids: 5-30%, urea: 5-30%, potassium primary phosphate: 5-20%, micro-2-10%, trace element are selected from copper, iron, manganese, zinc, boron, molybdenum.
To improvement of the technical scheme: in the B step, want earlier the level land ditching, cover the end or build the cement pond with plastic film, fresh Enteromorpha is stacked in the fermentation vat, the first layer thickness is 10-50 centimetre, the second layer and threeply degree all are 10-40 centimetre, spray fermented bacterium to the fresh Enteromorpha of first, second and third layer respectively, and the sprinkling amount of each layer fermented bacterium is the 3%-5% of the fresh Enteromorpha total weight percent of this layer; The fermentation vat top covers with plastic film and the yellow mud sealing then, causes the anaerobically fermenting environment.
To further improvement in the technical proposal: in the B step, 55 ℃ pliotherm period 5-6 days, 45 ℃ 5-6 days middle temperature phase, 35 ℃ hypothermic phase 12-15 days.
To further improvement in the technical proposal: in the C step, in the fermenting enteromorpha liquid of handling well, add humic acids, urea, potassium primary phosphate, trace element respectively,, make high reactivity Enteromorpha foliage fertilizer through 80-100 ℃ of heat inactivation 20-40 minute; Weight proportion is as follows:
Fermenting enteromorpha liquid: 10%-50%, humic acids: 10%-30%, urea: 10%-30%, potassium primary phosphate: 5%-20%, micro-3%-10%.
The present invention compared with prior art has many advantages and positively effect:
The present invention adopts specified strain to Enteromorpha decompositions of fermenting, and in the weak acid environment of microorganism growth breeding nature formation, makes softening of the cell wall; By vital movement of microorganism and the effect of its secreted various enzyme, make cell wall rupture, dissolving again, the effective constituent in the Enteromorpha is discharged fully.The Enteromorpha seaweed fertilizer of various dissimilar and proportionings is made in reinforcement by other nutritive ingredient again.
The bacterial classification that the present invention adopts is screening and optimize the bacterial strain that obtains in the Enteromorpha of spontaneous fermentation degraded, mainly contains compositions such as bacterium, actinomycetes and fungi, and sugar, protein, Mierocrystalline cellulose and organism are all had very strong capacity of decomposition.
Technology of the present invention is simple, and is with low cost, and the effective constituent stripping in the Enteromorpha is complete, do not add any strong acid, highly basic carries out forced hydrolysis, has kept Enteromorpha inherent nutritive ingredient fully.Complete with this all kinds of Enteromorpha seaweed organism fertilizer nutrition of making, the material that contains multiple promotion plant-growth, not only can strengthen the crop photosynthesis effect, increase crop yield, improve product quality, can also strengthen cold-resistant, drought resisting, the resistance of crop, reduce the generation of disease and pest, and can improve the physico-chemical property of soil, alleviate the continuous cropping continuous cropping disease.
Embodiment
The specific embodiment of the method for a kind of producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation of the present invention, method is as follows:
The preparation of A, Enteromorpha seed liquor
Bacterium, actinomycetes, fungi are carried out submerged fermentation respectively to be cultivated, bacterium, actinomycetes, fungal fermented filtrate are according to the 1:1:1 mixed, add urea, potassium primary phosphate, peptone, SODIUMNITRATE in the mixed solution and contain micro-salt, trace element is selected from copper, iron, manganese, zinc, boron, molybdenum, mixes to form the Enteromorpha seed liquor; Each weight percentages of components in the Enteromorpha seed liquor: urea 0.5%-10%, potassium primary phosphate 5%-20%, peptone 3%-5%, contain the trace element salt 3%-10%, all the other are the mixed solution of bacterium, actinomycetes, fungal fermented filtrate.
B, large scale fermentation are handled Enteromorpha
The level land ditching is covered the end or is built the cement pond with plastic film.Fresh Enteromorpha is stacked in the fermentation vat, the first layer thickness is 10-50 centimetre, the second layer and threeply degree all are 10-40 centimetre, spray fermented bacterium to the fresh Enteromorpha of first, second and third layer respectively, and the sprinkling amount of each layer fermented bacterium is the 3%-5% of the fresh Enteromorpha total weight percent of this layer; The fermentation vat top covers with plastic film and the yellow mud sealing then, causes the anaerobically fermenting environment.Whole anaerobic fermentation process is through pliotherm period leavening temperature (55 ℃, about 5 days), middle temperature phase (45 ℃, about 5 days), low temperature fermentation phase (35 ℃, about 15 days).After anaerobically fermenting finishes, open the sealing on top, at this moment Enteromorpha has become the semisolid of liquid or pasty state, need facultative aerobic fermentation again through about 5 days, the stink that in anaerobic fermentation process, produces, aerobic fermentation through microorganism has been transformed into carbonic acid gas and water, thereby has removed stink.Then fermenting enteromorpha liquid is filtered, the fermented supernatant fluid of handling well can directly use as seaweed fertilizer.
C, the preparation of Enteromorpha fertilizer
Enteromorpha foliage fertilizer: in the fermenting enteromorpha liquid of handling well, add humic acids, urea, potassium primary phosphate, trace element (being selected from copper, iron, manganese, zinc, boron, molybdenum) respectively, through (80-120 ℃ of heat inactivation, 20-40 minute), make high reactivity Enteromorpha foliage fertilizer; Weight proportion is as follows:
Fermenting enteromorpha liquid: 10%-50%, humic acids: 10%-30%, urea: 10%-30%, potassium primary phosphate: 5%-20%, micro-3%-10%.
Trace element in the foregoing description is the salt that adopts several elements in cupric, iron, manganese, zinc, boron, the molybdenum.
Certainly, above-mentioned explanation is not to be limitation of the present invention, and the present invention is not limited to above-mentioned giving an example, those skilled in the art, and in essential scope of the present invention, the variation of making, remodeling, interpolation or replacement all should belong to protection scope of the present invention.
Claims (6)
1, a kind of method of producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation is characterized in that, comprising:
The preparation of A, Enteromorpha seed liquor
Microbial strains is through the composite bacteria after screening and the domestication, is made up of bacterium, actinomycetes and fungi, all adapts to the higher environment of salinity in the fermenting enteromorpha; Sugar, protein, Mierocrystalline cellulose and organism all there is very strong capacity of decomposition;
Bacterium, actinomycetes, fungi are carried out submerged fermentation respectively to be cultivated, fermented liquid is formed according to bacterium: actinomycetes: fungi=1:1:1 mixes, in mixed fermentation liquid, add the necessary nutrition source of Enteromorpha biological degradation simultaneously as the biodegradable seed liquor of Enteromorpha, nutrition source is according to mixed fermentation liquid weight percent: nitrogen 0.2%-5%, phosphorus 2.5%-10%, potassium 1%-6% and micro-0.3-5%;
B, large scale fermentation are handled Enteromorpha
In fresh Enteromorpha, add the Enteromorpha biological degradation seed liquor of 1%-5% according to fresh Enteromorpha gross weight, cover by plastic film then and the mud sealing, cause the anaerobic environment of fermenting enteromorpha, utilize anaerobic bacterium in the bacterial classification Enteromorpha of degrading, whole anaerobic treatment process need time 20-30 days, wherein, 50-60 ℃ pliotherm period 1-10 days, 35-50 ℃ 3-10 days middle temperature phase, 28-35 ℃ hypothermic phase 10-15 days; After anaerobically fermenting finishes, open the sealing on top, carry out 3-15 days facultative aerobic fermentation, this process is utilized actinomycetes and the fungi in the bacterial classification, to further decompose less than the Enteromorpha that degraded is good in anaerobic fermentation process, and can remove foul smell in the anaerobic fermentation process, the effective constituent in the Enteromorpha is discharged fully, then fermenting enteromorpha liquid is filtered, as all kinds of seaweed fertilizer of feedstock production;
C, the preparation of Enteromorpha fertilizer
With fermenting enteromorpha liquid is at least a compatibility in base fluid and humic acids, urea, potassium primary phosphate, the trace element, stirs, and has just made seaweed fertilizer.
2, according to the method for the described producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation of claim 1, it is characterized in that: fermenting enteromorpha liquid: 10%-80%, humic acids: 5-30%, urea: 5-30%, potassium primary phosphate: 5-20%, trace element 2-10%, trace element is selected from copper, iron, manganese, zinc, boron, molybdenum.
3, according to the method for claim 1 or 2 described producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation, it is characterized in that: in the B step, want earlier the level land ditching, cover the end or build the cement pond with plastic film, fresh Enteromorpha is stacked in the fermentation vat, the first layer thickness is 10-50 centimetre, the second layer and threeply degree all are 10-40 centimetre, spray fermented bacterium to the fresh Enteromorpha of first, second and third layer respectively, the sprinkling amount of each layer fermented bacterium is the 3%-5% of the fresh Enteromorpha total weight percent of this layer; The fermentation vat top covers with plastic film and the yellow mud sealing then, causes the anaerobically fermenting environment.
4, according to the method for the described producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation of claim 3, it is characterized in that: in the B step, 55 ℃ pliotherm period 5-6 days, 45 ℃ 5-6 days middle temperature phase, 35 ℃ hypothermic phase 12-15 days.
5, according to the method for claim 1 or 2 described producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation, it is characterized in that: in the C step, in the fermenting enteromorpha liquid of handling well, add humic acids, urea, potassium primary phosphate, trace element respectively, through 80-100 ℃ of heat inactivation 20-40 minute, make high reactivity Enteromorpha foliage fertilizer; Weight proportion is as follows:
Fermenting enteromorpha liquid: 10%-50%, humic acids: 10%-30%, urea: 10%-30%, potassium primary phosphate: 5%-20%, micro-3%-10%.
6, according to the method for the described producing seaweed fertilizer by processing Entermorpha prolifera by microbial fermentation of claim 4, it is characterized in that: in the C step, in the fermenting enteromorpha liquid of handling well, add humic acids, urea, potassium primary phosphate, trace element respectively, through 80-100 ℃ of heat inactivation 20-40 minute, make high reactivity Enteromorpha foliage fertilizer; Weight proportion is as follows:
Fermenting enteromorpha liquid: 10%-50%, humic acids: 10%-30%, urea: 10%-30%, potassium primary phosphate: 5%-20%, micro-3%-10%.
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| CN101767921A (en) * | 2010-02-11 | 2010-07-07 | 中国水产科学研究院渔业机械仪器研究所 | Method for processing slurry with enteromorpha as coagulant aid |
| CN102173907A (en) * | 2011-01-30 | 2011-09-07 | 山东金利丰生物科技股份有限公司 | Preparation method of algae amino acid microbial fertilizer |
| CN102351577A (en) * | 2011-07-07 | 2012-02-15 | 青岛市环境卫生科研所 | Entermorpha aerobic composting processing method and system |
| CN102357501A (en) * | 2011-07-07 | 2012-02-22 | 青岛市环境卫生科研所 | Rapid harmless emergency treatment method of enteromorpha |
| CN102391030A (en) * | 2011-08-15 | 2012-03-28 | 上海理工大学 | Organic seaweed fertilizer and application thereof as efficient plant growth regulator |
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