CN114804960A - Fertilizer compounded by microalgae fermentation liquor and humic acid and preparation method thereof - Google Patents

Fertilizer compounded by microalgae fermentation liquor and humic acid and preparation method thereof Download PDF

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Publication number
CN114804960A
CN114804960A CN202210599003.6A CN202210599003A CN114804960A CN 114804960 A CN114804960 A CN 114804960A CN 202210599003 A CN202210599003 A CN 202210599003A CN 114804960 A CN114804960 A CN 114804960A
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microalgae
humic acid
fertilizer
fermentation liquor
parts
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赵璐
张咏
吴志刚
梁青
张新明
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Rizhao Polytechnic
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Rizhao Polytechnic
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12Unicellular algae; Culture media therefor

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pest Control & Pesticides (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Soil Sciences (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Botany (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to the technical field of bio-organic fertilizers, in particular to a fertilizer compounded by microalgae fermentation liquor and humic acid, which comprises the following components: microalgae fermentation liquor, humic acid, medium trace elements, composite bacteria powder, organic waste and water. The organic fertilizer contains microalgae, has the effect of promoting the growth of crops, and simultaneously improves the soil structure and the soil fertility under the coordination of humic acid; the invention utilizes the action of hypha generated by the propagation of the compound bacterial powder, can 'bind' the active ingredients such as microalgae and the like in soil, prevent the fertility from moving downwards and improve the utilization rate of the fertilizer; in the preparation method, the organic waste has the functions of providing a matrix for the composite bacterial powder to be beneficial to propagation on one hand, and changing the decomposed composite bacterial powder into nutrient components absorbed by crops on the other hand to promote the growth of the crops.

Description

Fertilizer compounded by microalgae fermentation liquor and humic acid and preparation method thereof
Technical Field
The invention relates to the technical field of bio-organic fertilizers, in particular to a fertilizer compounded by microalgae fermentation liquor and humic acid.
Background
The biological fertilizer is a product containing beneficial microorganisms, can promote substance conversion in soil and water through the life activities of the microorganisms, promote the absorption of the crops on nutrients in the environment, stimulate and regulate the growth of the crops, and simultaneously can prevent and control plant diseases and insect pests, thereby achieving the purpose of increasing the yield of the crops, and playing an important role in the aspects of providing nutrients for the crops, regulating the dynamic state of organic matters, enhancing the biological activity of the soil and the like. The biological fertilizer has the characteristics of trace amount, high efficiency and no harm to the environment, and the used microorganisms are environment-friendly, so that various environmental problems of water source pollution, soil quality damage and the like caused by chemical fertilizers can be avoided, and the biological fertilizer is an optimal substitute for the chemical fertilizers.
Microalgae are low autotrophic organisms with small individuals, simple structures and high photosynthetic efficiency, are widely distributed in different habitats such as soil, oceans, rivers, lakes and the like, and even live in some extreme environments (such as north and south poles, drought and saline-alkali soil and the like). There are many kinds of microalgae, and about 3 million different kinds of microalgae on the earth account for about 70% of known algae in the world, including prokaryotes such as cyanophyta, and eukaryotes such as chlorophyta and diatom. The advantages of microalgae as a biofertilizer are the following: (1) the nutrient can prevent nutrient loss by slowly releasing nitrogen, phosphorus and potassium, and meets the growth requirement of plants; (2) besides containing a large amount of nutrient elements, the fertilizer also contains trace elements and substances for promoting plant growth, such as plant hormones, vitamins, carotenoids, amino acids and antifungal substances, which can promote the growth of crops and improve the soil fertility.
The humic substances are produced by dead biological substances such as lignin through microbial degradation and are difficult to further degrade, and are widely applied to the production of organic fertilizers, and CN202110684136.9 discloses a liquid water-soluble fertilizer prepared by compounding microalgae fermentation liquor and humic acid and a production method thereof.
Disclosure of Invention
Aiming at the problems of low utilization rate of the microalgae organic fertilizer and the like in the prior art, the invention provides the fertilizer compounded by the microalgae fermentation liquid and humic acid, which can bind the effective components of the microalgae organic fertilizer, prevent the fertilizer efficiency from moving downwards and improve the utilization rate of the fertilizer.
In a first aspect, the invention provides a fertilizer compounded by microalgae fermentation liquor and humic acid, which comprises the following components: microalgae fermentation liquor, humic acid, medium trace elements, composite bacteria powder, organic waste and water.
Further, the composition comprises the following components in parts by weight: 50-70 parts of microalgae fermentation liquor, 10-20 parts of humic acid, 5-10 parts of medium trace elements, 3-5 parts of composite bacterial powder, 20-30 parts of organic waste and 30-80 parts of water.
Further, the microalgae fermentation liquor is prepared by the following method, microalgae is inoculated on a culture medium and is obtained by heterotrophic fermentation, and the culture medium comprises the following components: glucose, sodium acetate, peptone, beef extract, yeast extract powder, urea and B vitamins.
Further, the microalgae is one of blue algae, cryptophyceae, chrysophyceae, yellow algae and diatoms.
Further, the medium trace elements are one or more of manganese sulfate, magnesium sulfate, zinc sulfate, calcium nitrate and potassium dihydrogen phosphate.
Further, the composite bacterial powder is mixed bacterial powder of bacillus bacterial powder and trichoderma bacterial powder, wherein the mass ratio of the bacillus bacterial powder to the trichoderma bacterial powder is 1-3: 1.
Further, the organic waste comprises cow dung, straws, wheat bran, rice hulls, bone meal and bean dregs.
In a second aspect, the invention provides a preparation method of a fertilizer compounded by microalgae fermentation liquor and humic acid, which comprises the following steps:
(1) carrying out compost fermentation on humic acid, compound bacteria powder and organic waste, and turning over and ventilating in the fermentation process to obtain a compost fermented fertilizer;
(2) and fully mixing and stirring the compost fermented fertilizer, microalgae fermentation liquor, medium trace elements and water to obtain a finished water-soluble fertilizer.
Further, in the step (1), the compost is fermented for 3-5 days, the compost is turned over once every day, and the fermentation temperature is 30-35 ℃.
The invention has the beneficial effects that:
(1) the organic fertilizer contains microalgae, has the effect of promoting the growth of crops, and simultaneously improves the soil structure and the soil fertility under the coordination of humic acid;
(2) the invention utilizes the action of hypha generated by the propagation of the compound bacterial powder, can 'bind' the active ingredients such as microalgae and the like in soil, prevent the fertility from moving downwards and improve the utilization rate of the fertilizer;
(3) in the preparation method, the organic waste has the functions of providing a matrix for the composite bacterial powder to be beneficial to propagation on one hand, and changing the decomposed composite bacterial powder into nutrient components absorbed by crops on the other hand to promote the growth of the crops.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
In examples 1-3, the microalgae fermentation broth is obtained by inoculating cyanobacteria on a culture medium and performing heterotrophic fermentation, wherein the culture medium comprises the following components: glucose, sodium acetate, peptone, beef extract, yeast extract powder, urea and B vitamins.
Example 1
The preparation method of the fertilizer compounded by the microalgae fermentation liquor and the humic acid comprises the following steps:
(1) composting and fermenting 10 parts of humic acid, 3 parts of composite bacteria powder and 20 parts of organic waste for 3-5 days, turning over once every day at the fermentation temperature of 30-35 ℃, and ventilating in the fermentation process to obtain a compost fermented fertilizer, wherein the composite bacteria powder is mixed bacteria powder of bacillus bacteria powder and trichoderma bacteria powder with the mass ratio of 1:1, and the organic waste comprises cow dung, straw, wheat bran, rice hulls, bone meal and bean dregs;
(2) and fully mixing and stirring the compost fermented fertilizer, 50 parts of microalgae fermentation liquor, 5 parts of medium trace elements and 30 parts of water, wherein the medium trace elements are manganese sulfate, magnesium sulfate, zinc sulfate, calcium nitrate and potassium dihydrogen phosphate, and obtaining a finished product of the water-soluble fertilizer.
Example 2
The preparation method of the fertilizer compounded by the microalgae fermentation liquor and the humic acid comprises the following steps:
(1) composting and fermenting 15 parts of humic acid, 4 parts of composite bacterial powder and 25 parts of organic waste for 3-5 days, turning over once every day at the fermentation temperature of 30-35 ℃, and ventilating in the fermentation process to obtain a compost fermented fertilizer, wherein the composite bacterial powder is mixed bacterial powder of bacillus bacterial powder and trichoderma bacterial powder with the mass ratio of 3:1, and the organic waste comprises cow dung, straw, wheat bran, rice hulls, bone meal and bean dregs;
(2) and fully mixing and stirring the compost fermented fertilizer with 60 parts of microalgae fermentation liquor, 7 parts of medium trace elements and 50 parts of water, wherein the medium trace elements are manganese sulfate, magnesium sulfate, zinc sulfate, calcium nitrate and potassium dihydrogen phosphate, and obtaining a finished product of the water-soluble fertilizer.
Example 3
The preparation method of the fertilizer compounded by the microalgae fermentation liquor and the humic acid comprises the following steps:
(1) composting and fermenting 20 parts of humic acid, 5 parts of composite bacterial powder and 30 parts of organic waste for 3-5 days, turning once every day at the fermentation temperature of 30-35 ℃, and ventilating in the fermentation process to obtain a compost fermented fertilizer, wherein the composite bacterial powder is mixed bacterial powder of bacillus bacterial powder and trichoderma bacterial powder with the mass ratio of 2:1, and the organic waste comprises cow dung, straw, wheat bran, rice hulls, bone meal and bean dregs;
(2) and fully mixing and stirring the compost fermented fertilizer with 70 parts of microalgae fermentation liquor, 10 parts of medium trace elements and 80 parts of water, wherein the medium trace elements are manganese sulfate, magnesium sulfate, zinc sulfate, calcium nitrate and potassium dihydrogen phosphate, and obtaining a finished product of the water-soluble fertilizer.
Comparative example 1
Referring to example 1, the difference between comparative example 1 and example 1 is that the fertilizer component of comparative example 1 does not contain compound bacterial powder, the rest components and the proportion thereof are the same, and the finished product is obtained by directly compounding in step (2).
Test examples
Using tomato crops as an example, the organic fertilizer obtained in the examples and the comparative examples is fertilized according to 100 kg/mu, the fertilizer is fertilized twice before the plants bloom, and the fertilizer is fertilized once after the plants bloom, and the growth conditions and the fruiting yield of the plants are shown in Table 1.
Table 1 examples and comparative examples plant growth and fruiting yield
Item Density (plant/mu) Plant height (cm) Stem diameter (cm) Number of results (number/strain) Weight of single melon (g)
Example 1 3500 75.3 1.89 7.8 178
Example 2 3500 74.5 1.90 7.6 163
Example 3 3500 76.3 2.0 8.0 170
Comparative example 1 3500 70.3 1.60 6.0 120
As can be seen from Table 1, the present invention can promote the growth of crops and increase the yield of fruits.
Although the present invention has been described in detail by way of preferred embodiments, the present invention is not limited thereto. Various equivalent modifications or substitutions can be made on the embodiments of the present invention by those skilled in the art without departing from the spirit and scope of the present invention, and these modifications or substitutions are within the scope of the present invention/any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention.

Claims (9)

1. A fertilizer compounded by microalgae fermentation liquor and humic acid is characterized by comprising the following components: microalgae fermentation liquor, humic acid, medium trace elements, composite bacteria powder, organic waste and water.
2. The fertilizer compounded by microalgae fermentation liquor and humic acid as claimed in claim 1, is characterized by comprising the following components in parts by weight: 50-70 parts of microalgae fermentation liquor, 10-20 parts of humic acid, 5-10 parts of medium trace elements, 3-5 parts of composite bacterial powder, 20-30 parts of organic waste and 30-80 parts of water.
3. The fertilizer compounded by microalgae fermentation liquor and humic acid as claimed in claim 1, wherein the microalgae fermentation liquor is prepared by inoculating microalgae on a culture medium and performing heterotrophic fermentation, and the culture medium comprises the following components: glucose, sodium acetate, peptone, beef extract, yeast extract powder, urea and B vitamins.
4. The fertilizer compounded by microalgae fermentation liquor and humic acid as claimed in claim 3, wherein the microalgae is one of blue algae, cryptomonas, chrysophyceae, xanthomonas and diatom.
5. The fertilizer compounded by microalgae fermentation liquor and humic acid as claimed in claim 1, wherein the medium trace element is one or more of manganese sulfate, magnesium sulfate, zinc sulfate, calcium nitrate and potassium dihydrogen phosphate.
6. The fertilizer compounded by microalgae fermentation liquor and humic acid as claimed in claim 1, wherein the compound bacteria powder is mixed bacteria powder of bacillus bacteria powder and trichoderma bacteria powder, wherein the mass ratio of the bacillus bacteria powder to the trichoderma bacteria powder is 1-3: 1.
7. The fertilizer compounded by microalgae fermentation liquor and humic acid as claimed in claim 1, wherein the organic waste comprises cow dung, straw, wheat bran, rice hull, bone meal and bean dregs.
8. The preparation method of the fertilizer compounded by microalgae fermentation liquor and humic acid as claimed in claim 1, is characterized by comprising the following steps:
(1) carrying out compost fermentation on humic acid, compound bacteria powder and organic waste, and turning over and ventilating in the fermentation process to obtain a compost fermented fertilizer;
(2) and fully mixing and stirring the compost fermented fertilizer, microalgae fermentation liquor, medium trace elements and water to obtain a finished water-soluble fertilizer.
9. The preparation method of the fertilizer compounded by the microalgae fermentation liquid and the humic acid according to claim 8, characterized in that in the step (1), the fermentation time of the compost is 3-5 days, the compost is turned over once every day, and the fermentation temperature is 30-35 ℃.
CN202210599003.6A 2022-05-30 2022-05-30 Fertilizer compounded by microalgae fermentation liquor and humic acid and preparation method thereof Pending CN114804960A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115215692A (en) * 2022-08-18 2022-10-21 天津大学 Microbial diatom organic fertilizer and preparation method and application thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102765995A (en) * 2012-08-16 2012-11-07 史丹利化肥股份有限公司 Efficient multifunctional seaweed biological fertilizer
CN104261981A (en) * 2014-09-19 2015-01-07 南京农业大学 Preparation method of seaweed organic fertilizer and organic fertilizer prepared by using method
CN108484263A (en) * 2018-04-12 2018-09-04 罗永城 A kind of preparation method and application method of efficient microalgae foliar fertilizer
CN112409101A (en) * 2020-11-20 2021-02-26 内蒙古丰沃达生物科技有限公司 Biological organic fertilizer and preparation method thereof
CN113307687A (en) * 2021-06-21 2021-08-27 北京耕天下生物科技有限公司 Liquid water-soluble fertilizer compounded by microalgae fermentation liquor and humic acid and production method thereof

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102765995A (en) * 2012-08-16 2012-11-07 史丹利化肥股份有限公司 Efficient multifunctional seaweed biological fertilizer
CN104261981A (en) * 2014-09-19 2015-01-07 南京农业大学 Preparation method of seaweed organic fertilizer and organic fertilizer prepared by using method
CN108484263A (en) * 2018-04-12 2018-09-04 罗永城 A kind of preparation method and application method of efficient microalgae foliar fertilizer
CN112409101A (en) * 2020-11-20 2021-02-26 内蒙古丰沃达生物科技有限公司 Biological organic fertilizer and preparation method thereof
CN113307687A (en) * 2021-06-21 2021-08-27 北京耕天下生物科技有限公司 Liquid water-soluble fertilizer compounded by microalgae fermentation liquor and humic acid and production method thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115215692A (en) * 2022-08-18 2022-10-21 天津大学 Microbial diatom organic fertilizer and preparation method and application thereof

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