CN101485287A - Method for production of maize hybrid seeds - Google Patents
Method for production of maize hybrid seeds Download PDFInfo
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- CN101485287A CN101485287A CN 200910079033 CN200910079033A CN101485287A CN 101485287 A CN101485287 A CN 101485287A CN 200910079033 CN200910079033 CN 200910079033 CN 200910079033 A CN200910079033 A CN 200910079033A CN 101485287 A CN101485287 A CN 101485287A
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Abstract
The invention belongs to the field of heterosis utilization, and particularly relates to a seed preparation method for corn hybrid species. The method takes inbred M56 as a female parent and takes inbred S121 as a male parent to prepare a corn hybrid species, Nonghua 101, wherein the inbred M56 is obtained through continuous inbred 8-generation seed selection after equivalent dosages of pollen of the US 1160R, 1140D and X1132X are mixed and the mixed pollen is pollenized to a Germany high quality hybrid species 72A16 so as to acquire an F1 generation, and the inbred S121 is obtained through the continuous 6-generation inbred of good individual strains selected from an implanting P2 group after the pollen of Dan 340, H201 and H204 is mixed for obtaining the filial generation and the open pollination among an implant small group is carried out. The hybrid species is characterized by high yield, stable yield, high disease resistance, and high biological output.
Description
Technical field
The present invention relates to a kind of hybrid maize seed production method, it belongs to crops corn hybrid use of advantage field.
Background technology
Corn germ plasm resource is narrow, the development and the progress of China's corn breeding and production have seriously been hindered, China is the crossbreed of institute's seed selection in the period of 20 in the past, its parental inbred line derives from Lancaster, the yellow gingival cyst of mucous gland in the newborn of auspicious moral, the red bone in Luda and Tang Si tack four systems mostly, this not only exists hereditary fragility problem, and the inbred line of seed selection and hybrid combination are difficult to big breakthrough still all not having characteristic on output on the proterties.For the bigger food needs that satisfy the growth in the Chinese people, and the needs of corn deep processing product, breeding high-yield, many anti-, wide suitable corn varieties are the top priorities of present China corn breeding circle.
China's corn breeding historys teaches that: " introducing of external excellent resources and application are two China's corn breeding and production developments and one of progressive major reason during the last ten years ", so should pay attention to very much the introduction of use of external germplasm.More domestic corn breeding units successively directly and the external germplasm of indirect utilization select a collection of elite hybrid, as middle list 2, red beautiful 13, red beautiful 15, tuck in single series, agricultural university's series, Zheng's single series, China's germ plasm resource is not only enriched in the introduction of external germplasm and utilization, also can create new hybrid vigour pattern, select breakthrough hybrid combination.
Summary of the invention
The objective of the invention is to utilize the hybrid vigour theory, select high-combining ability, economical character is good, genotype is isozygotied, parents' affiliation is far away hybridization between selfed lines for use, it is good to select quality, the output height, and dehydration is fast, the corn hybrid seed disease-resistant, that lodging resistance is good.
The invention provides a kind of method of hybrid maize seed production, comprise the seed selection inbred line, safety is isolated, and regulates date of seeding, suitable row ratio, roguing, the maternal castration rooted out male parent, results utilize self bred inbred line M56 to be female parent in good time, and drawing S121 outward is paternal hybrid, the preparation corn hybrid seed.
Wherein said self bred inbred line M56 mixes U.S. 1160R, 1140D, X1132X (all drawing from the U.S. in calendar year 2001) equivalent pollen, and pollen mixture is invested German precocious elite hybrid 72A16, and 8 generations of selfing are bred continuously behind the results seed;
Draw outward be S121 be by red 340 with the mixed powder filial generation of H201 and H204, the open pollination of plantation microcommunity, plantation P2 colony selects 6 generations of the continuous selfing of fine individual plant to breed.
Biomaterial M56, the S121 that the present invention relates to is all open in non-patent literature, and above-mentioned biomaterial all has preservation at the applicant place, and the applicant guarantees to provide to the public in 20 years present patent application days.
The 26th page of " Ministry of Agriculture's new variety of plant protection bulletin " that M56 has published on May 1st, 2009 3 phase in 2009, put down in writing it and be applied to breeding as parent material.
" Ministry of Agriculture's new variety of plant protection bulletin " the 3rd phase in 2009 that S121 has published on March 1st, 2009, put down in writing it for the 20th page and be applied in the breeding as parent material.
The present invention utilizes self bred inbred line M56 to be female parent, and drawing outward is that S121 is the feature of the corn hybrid seed farming China 101 of paternal hybrid preparation: breeding time: 110-125 days (summer sowing district-spring sowing district), plant height 2.8m-3.1m; fringe position 0.9m-1.1m, fruit ear cartridge type, spike length 18.2cm; the thick 4.9cm of fringe; 16-18 is capable, seed yellow, horse flute profile; the cob purple; the flower pesticide pink, 100-grain weight 29.8-33.2g, seed-producing rate 84.8-86.5g.
The feature of parent's self bred inbred line M56: 124 days breeding times, plant height 2.4m, fringe position 0.8m; the long cartridge type of fruit ear, spike length 19.5cm, head progeny row 12-14 row; the yellow horse flute profile of seed, cob purple, filigree redness; the flower pesticide yellow; plant type is delicate and pretty, and internode is long, 6-7 of fringe blades; 100-grain weight 35.2g, seed-producing rate 86.8g.
It is S121 that the parent draws outward: 126 days breeding times, plant height 1.6m, fringe position 0.7m, the thick cartridge type of fruit ear, spike length 12.5cm; tassel row number 16-18 row, seed yellow, horse flute profile, cob white, filigree green; the flower pesticide pink colour, the compact upper punch of plant type, 100-grain weight 36g, seed-producing rate 84.5g.
Advantage of the present invention and good effect are:
1. output height: 2005 through Dandong, Liaoning Province, Changtu, Siping City, Jilin Province, sub-district, Changchun variety comparative test: four some average yield (666.7m
2) 728.38 kilograms, comparison is according to kind of red beautiful No. 39 volume increase 6.58%; Than Zheng Dan 958 volume increase 8.66%, than stepping on No. 9 volume increase 11.69% in sea.
2006 through Dandong, Liaoning Province, Tieling, Changtu, Siping City, Jilin Province, Gongzhuling, agricultural peace, elm, Baicheng, Shunyi, Beijing, 10 points in Laizhou, Shandong Province time sub-district variety comparative test, 758.8 kilograms of (666.7m of average yield
2), comparison is according to planting: red beautiful No. 39 (3 points) on average increase production 5.89%; On average increase production 7.28% than Zheng Dan 958 (8 points); (3 points) on average increases production 10.37% than stepping on the sea No. 9, on average increases production 3.84% than beautiful 335 (3 points) of elder generation.
2. best in quality: the outward appearance commodity is good, cutin content height, the grain of selling well.
3. resistance.
4. dehydration is fast: energy fast dewatering, threshing after the results.
5. wide adaptability: be fit to the northwest, eastern North China, ground such as the Yellow River and Huai He River sea.
Description of drawings
Fig. 1 is a hybrid seeding family tree of the present invention.
Embodiment
Following examples are used to illustrate the present invention, but are not used for limiting the scope of the invention.
Embodiment 1: the concrete seed selection process of crossbreed farming magnificent 101 of the present invention
Agricultural China 101 is that Beijing agricultural Hua Zhongye of gold company was maternal with self bred inbred line M56 in 2005, and drawing outward is that S121 is a male parent, and the assembly of assorted effect is bred.Wherein M56 mixes U.S. 1160R, 1140D, X1132X (all drawing from the U.S. in calendar year 2001) equivalent pollen, and pollen mixture is invested German precocious elite hybrid 72A16, and 8 generations of selfing are bred continuously behind the results seed; Male parent S121 by red 340 with the mixed powder filial generation of H201 and H204, the open pollination of plantation microcommunity, plantation P2 colony selects 6 generations of the continuous selfing of fine individual plant to breed.
1) output performance: farming China 101,2006 years through Dandong, Liaoning Province, Tieling, Changtu, Siping City, Jilin Province, Gongzhuling, agricultural peace, elm, Baicheng, Shunyi, Beijing, 10 points in Laizhou, Shandong Province time sub-district variety comparative test, 758.8 kilograms of (666.7m of average yield
2), comparison is according to planting: red beautiful No. 39 (3 points) on average increase production 5.89%; On average increase production 7.28% than Zheng Dan 958 (8 points); (3 points) on average increases production 10.37% than stepping on the sea No. 9, on average increases production 3.84% than beautiful 335 (3 points) of elder generation.Rate schedule sees Table 1,2.
Table 1.2008 year eastern North China preliminary experiment farming magnificent 101 yield result units: KG
| Hold examination unit | Per mu yield | Contrast red beautiful output | Than CK ± |
| Academy of agricultural sciences, Liaoning Province | 768.8 | 715 | 7.45 |
| The academy of agricultural sciences, Tieling | 912.1 | 800.6 | 13.92 |
| The academy of agricultural sciences, Jinzhou, Liaoning | 718.9 | 650.9 | 10.44 |
| The academy of agricultural sciences, Dalian | 525.9 | 471.8 | 11.47 |
| The Jilin milky way | 757.8 | 679.0 | 11.60 |
| Jilin agricultural university | 996.2 | 913.1 | 9.10 |
| The Jiutai | 777.3 | 608.7 | 27.70 |
| The Hebei honorization | 728.7 | 713.7 | 2.10 |
| The Chengde | 846.3 | 791.9 | 6.87 |
| The village, Shanxi is beautiful | 816.8 | 805.5 | 1.40 |
| Fenyang, Shanxi | 610.8 | 564.5 | 8.20 |
| The Tongliao | 596 | 659.4 | -9.61 |
| The Chifeng | 994.8 | 910.2 | 9.30 |
| The yan-an | 795.4 | 771.4 | 3.11 |
| Overall average | 774.7 | 716.7 | 8.1 |
Table 2.2008 year the Yellow River and Huai He River sea preliminary experiment farming magnificent 101 yield result units: KG
| Hold examination unit | Per mu yield | Contrast Zheng Dan 958 output | Than CK ± |
| Shijiazhuang, Hebei | 680.4 | 635.4 | 7.09 |
| Baofeng, Henan | 578.7 | 585.9 | -1.23 |
| Zhengzhou, Henan | 714.6 | 707.6 | 0.99 |
| Yuncheng, Shanxi | 930.2 | 848.5 | 9.63 |
| Luoyang, Henan | 559.0 | 595.3 | -6.09 |
| The Changqing, Shandong | 639.6 | 488.9 | 30.84 |
| Cangzhou, Hebei | 729.3 | 671.4 | 8.63 |
| The Pingyi, Shandong | 701.3 | 680.2 | 3.10 |
| Distributions in Liaocheng of Shandong Province | 919.8 | 878.9 | 4.66 |
| Dezhou, Shandong | 651.7 | 554.1 | 17.61 |
| Zaozhuang, Shandong | 555.4 | 577.8 | -3.88 |
| On average | 589.2 | 558.5 | 5.49 |
2), resistance
The disease-resistant worm of Institute of Crop Science, Chinese Academy of Agricultural Science was identified summarized results in 2008: 1 grade of leaf blight, 7 grades of helminthosporium maydis, 5 grades of Curvularia lunata leaf spots, high anti-head-smut, high-resistance corn snout moth's larva.Resistance the results are shown in Table 3,4,5.
Table 3.2008 year Dong Huabeiqu is through Institute of Crop Science, Chinese Academy of Agricultural Science's disease resistance qualification result:
Table 4.2008 year Dong Huabeiqu is through Institute of Crop Science, Chinese Academy of Agricultural Science's disease resistance qualification result:
The southwestern Qu Jing of table 5 2008 Institute of Crop Science, Chinese Academy of Agricultural Science disease resistance qualification result:
Embodiment 2: the seeding technique of crossbreed farming magnificent 101 of the present invention
1, guarantees that safety isolates: can not plant other any corn crop with interior for 300 meters around the seed farm.
2, the sowing same period: M56 does female parent, and S121 does male parent, simultaneously sowing.
3, suitable row ratio: father, maternal going than adopting 1:5,1 row male parent is planted at the maternal interval of promptly per 5 row, the maternal 6000 strain/mus that keep, male parent keeps 1200 strain/mus, and its output is more than 500 kilograms, and the high-yield field piece can reach 600 kilograms.
4, removal of impurities is rogued: before father, female parent are weaved silk, and all the assorted strains in Ex-all father, the maternal row, variation is planted.
5, the maternal castration rooted out male parent.The maternal castration is the key link of production of hybrid seeds work, is the necessary means that obtains pure hybrid seed, must employing take off luxuriant emasculating method in advance, accomplishes thorough, clean.After the female parent pollination in production of hybrid seeds district finishes, male parent is all rooted out, help ventilation and penetrating light, improve output, prevent to mix, ensure the quality of products.
6, gather in the crops the airing keeping: in time gather in the crops after the production of hybrid seeds district corn maturation, avoid meeting germination of rain fruit ear or mould grain, after drying, register warehouse-in and take care of in good time.Results when ripe " black layer " appears in bract flavescence, seed.
7, diseases prevention worm: plant clothing agent (Zhuozhou, Beijing (Hai Li) kind clothing agent Co., Ltd) with 16% good fortune gram azoles alcohol (black Mi Jing) and carry out Cotton seeds, the typhon mouth phase can add 0.7% urea (east, Chengdu aurification test agent Co., Ltd) control of maize snout moth's larva and male parent aphid with 0.4% potassium dihydrogen phosphate.
Embodiment 3: the culture technique of crossbreed farming magnificent 101 of the present invention
1, Suitable Density: sowing in spring in 1. eastern North China is 4000 strain/mus, and 2. the Yellow River and Huai He River sea summer sowing is 4500 strain/mus.
2, Fertilization Level: this kind moderate growth duration, spring, summer sowing all can, it is earlier beautiful 335 that disease-resistant, lodging resistance all is better than, yielding ability is better than Zheng Dan 958, anti-ridge is stronger, under conditions of high density, notes enriching farmyard manure, strengthens water, fertile management.
3, the corn lobus cardiacus will carry out the control of maize borer latter stage.
Claims (9)
1, a kind of hybrid maize seed production method comprises the seed selection inbred line, and safety is isolated, and regulates date of seeding, suitable row ratio, roguing, the maternal castration rooted out male parent, gather in the crops, it is characterized in that: utilize self bred inbred line M56 to be female parent, drawing outward is that S121 is a paternal hybrid in good time, the preparation corn hybrid seed.
2, the method for claim 1 is characterized in that, described self bred inbred line M56 is that continuous selfing 8 generation seed selection becomes after mixing powder with U.S. crossbreed 1160R, 1140D, X1132X, German crossbreed 72A163.
3, the method for claim 1 is characterized in that, described outer draw be S121 be by red 340 with the mixed powder of H201 and H204 after choosing be to breed in 6 generations of selfing.
4, the method for claim 1, it is characterized in that described safety isolation, adopt distance isolation or time to isolate, adopt distance isolation to require not plant other corn more than 300 meters around the production of hybrid seeds district, adopt in the time insulation request production of hybrid seeds district sowing time will with other corn mistake phase more than 20 days.
5, the method for claim 1 is characterized in that father and mother sow this same period.
6, the method for claim 1 is characterized in that described suitable row than being father, maternal going than adopting 1:5.
7, the method for claim 1 is characterized in that described roguing adopts in seedling stage, shooting stage, takes out male early stage and all do not pull out meeting the assorted strain of parent's characteristic feature characteristic, different strain, lopsided strain, diseased plant and weak strain.
8, the method for claim 1 is characterized in that described maternal the castration, adopts and takes off luxuriant emasculating method in advance, thoroughly extracts maternal tassel.
9, the method for claim 1 is characterized in that the described male parent of rooting out is after the female parent pollination in production of hybrid seeds district finishes, and male parent is all rooted out.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106254A (en) * | 2010-12-21 | 2011-06-29 | 中国农业大学 | Hybrid seed production method for nitrogen efficient maize |
| CN104885910A (en) * | 2014-09-29 | 2015-09-09 | 安徽盛创农业科技有限公司 | High-yield hybrid maize seed production method |
| CN115623982A (en) * | 2022-09-09 | 2023-01-20 | 邯郸市农业科学院 | Breeding method of drought-tolerant and disease-resistant corn |
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2009
- 2009-03-04 CN CN 200910079033 patent/CN101485287B/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102106254A (en) * | 2010-12-21 | 2011-06-29 | 中国农业大学 | Hybrid seed production method for nitrogen efficient maize |
| CN102106254B (en) * | 2010-12-21 | 2012-07-18 | 中国农业大学 | Hybrid seed production method for nitrogen efficient maize |
| CN104885910A (en) * | 2014-09-29 | 2015-09-09 | 安徽盛创农业科技有限公司 | High-yield hybrid maize seed production method |
| CN115623982A (en) * | 2022-09-09 | 2023-01-20 | 邯郸市农业科学院 | Breeding method of drought-tolerant and disease-resistant corn |
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Address after: 100080, Zhongguancun building, No. 27 Zhongguancun street, Beijing, Haidian District, China, 14 floor Patentee after: BEIJING KINGS NOWER SEED S&T CO.,LTD. Address before: 100080, Zhongguancun building, No. 27 Zhongguancun street, Beijing, Haidian District, China, 14 floor Patentee before: BEIJING JINSE NONGHUA SEED SCIENCE & TECHNOLOGY Co.,Ltd. |
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