CN101480156A - Method for using fungal biocontrol agents - Google Patents
Method for using fungal biocontrol agents Download PDFInfo
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- CN101480156A CN101480156A CNA2009100958753A CN200910095875A CN101480156A CN 101480156 A CN101480156 A CN 101480156A CN A2009100958753 A CNA2009100958753 A CN A2009100958753A CN 200910095875 A CN200910095875 A CN 200910095875A CN 101480156 A CN101480156 A CN 101480156A
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Abstract
The invention discloses a biocontrol fungi implementing method which belongs to the application of microorganisms. The prior implementing method of biocontrol fungus products needs too much labor and has poor spore germination and non-ideal colonization. In the biocontrol fungi implementing method, biocontrol fungus agents comprising biocontrol fungus spores are scattered on wet soil surface, and biocontrol fungi naturally disperse and propagate. Only the scattering of the biocontrol fungus agents on the wet soil surface is needed, thus the method is simple and easy to implement, saves labor and is easy to be accepted by users, and each acre of soil only needs 1.5 kg to 10 kg of biocontrol fungus so as to reduce the cost by several ten times. After the method is implemented, a nutrient substrate supplies nutrition to the biocontrol fungi so that the biocontrol fungi can grow and propagate on the soil surface for preventing the fungi of the roots and the stem bases of plants and also preventing the fungi of the stems, the leaves, the flowers and the fruits of the plants. After implementation, the biocontrol fungi implementing method also has a certain yield-increasing effect.
Description
Technical field
The invention belongs to the microorganism application, be specifically related to the application process of biocontrol fungi agent.
Background technology
Now, fungal diseases of plants has restricted high yield, stable yields and the high-quality of crop.The raising day by day that pollution-free, nuisanceless green organic food is required along with people, expert, scholar are devoted to not have the residual biological control research of chemical pesticide and do one's utmost, and filtered out that wooden mould, sticking broom is mould, the shield shell is mould, chactomium globosum, Paecilomyces lilacinus etc. some have antagonism disease fungi fungi function, that can be used for biological control and (be called for short: biocontrol fungi).What develop in recent years, register both at home and abroad is that the biocontrol fungi agent quantity of bacterial classification also has certain scale with the biocontrol fungi, but fails so far to be widely used on agricultural, and studying carefully in reason has two, first preparation form; It two is application processes, and these two is to be mutually related.
Aspect the preparation form, existing most biocontrol fungi product is the spore product, on application process mainly is to mix soil to spread fertilizer over the fields, directly admix plow layer, plant plant hole or spray.Because the spore of biocontrol fungi becomes current shorter, in the transportation of commodity preparation with in storing the part inactivation is arranged, under the situation that the spore amount of living reduces, above-mentioned application process influences sprouting, the growth of biocontrol fungi and grows surely.
On application process, as mix that soil spreads fertilizer over the fields, directly admixes plow layer, not only recruitment is too big to plant plant hole, and because of being subjected to the influence of the antibacterial factor and residue of pesticide in the soil, spore germination, mycelial growth are poor, grow undesirable surely.Spray in this way, limit to because of being subjected to weather, environmental influence and spraying technology own, the biocontrol fungi spore will be sprouted, grow surely more difficult at stem, leaf, flower, the fruit position of crop.
Summary of the invention
The technical assignment of the technical problem to be solved in the present invention and proposition be overcome biocontrol fungi product that prior art exists to use recruitment sprouting too big, spore poor, growth, grow shortcomings such as undesirable surely, a kind of application process of biocontrol fungi agent is provided.For this reason, the present invention is by the following technical solutions:
The application process of biocontrol fungi agent is characterized in that the biocontrol fungi agent that comprises the biocontrol fungi spore is spread fertilizer over the fields on the damp soil surface, leave biocontrol fungi naturally fast, raised growth, expansion be numerous.
The invention has the beneficial effects as follows:
One, the former method of using the biocontrol fungi agent are to mix native hundreds of times to spread fertilizer over the fields or admix plow layer, plant plant hole with several ratio of hundreds of branches, and such application process recruitment is too big, and practical application is had any problem.
The biocontrol fungi agent method of using of the present invention is as long as spread fertilizer over the fields to the plow layer surface of humidity, and method is simple, and recruitment province allows the user accept easily.
Two, former amount of application is excessive, and by plow layer, plant plant hole and mix and excute a law, every mu of the consumption of solid powder is more than double centner, and cost is higher.
Use the present invention, every mu only needs with 1.5 kilograms to 10 kilograms, and cost alleviates tens of times.
Three, effect aspect, the biocontrol fungi agent is mixed soil and is spread fertilizer over the fields, admixes plow layer, plants plant hole, method such as spray, be subjected to the influence of the antibacterial factor in the soil, spore germination is inconsistent, be difficult to reach general effect of comprehensively growing surely, add commodity preparation fungal spore part inactivation in the storage transportation, influence protection effect.
Implementing advantage of the present invention is, by expanding numerous effect, promptly works as the biocontrol fungi agent and is sprinkling upon the damp soil surface, the very fast moisture absorption of biocontrol fungi agent, under suitable temperature, endospore sprouting in 6 hours, mycelial growth, just can grow into bacterium colony in 12 hours, bacterium colony can produce spore in 24 hours.The biocontrol fungi agent was spread fertilizer over the fields in one week of back, and every gram can produce spore more than 1,000,000,000.These fresh and alive spores are with flow air between crop, are diffused into each corner of soil surface and stem of plant, leaf, flower, really, sprout in suitable place, growth and growing surely.
In the said process, the hypha,hyphae of bacterium colony bottom can be grown in soil fast because of obtaining the abundant nutrition of nutrition base, grows in soil surely.
Biocontrol fungi agent dispensing can obtain sufficient oxygen, and not be subjected to the influence of the antibacterial factor in the soil at soil surface, and growth rate is than fast several times in soil, and this is of the present invention one big characteristics.
Implementing the present invention prevents the mechanism of crop root, basal part of stem fungal disease to be, biocontrol fungi agent dispensing is being grown fast behind the soil surface, is being bred and growing surely in soil, suppress the sprouting of disease fungal spore in the soil and the growth of disease hypha,hyphae, inhibition disease fungi is infected crop, reaches the purpose that the prevention crop fungal disease takes place.
Implement the present invention and prevent stem of plant, leaf, flower if really the mechanism of fungus diseases is, biocontrol fungi is grown fast, is bred at soil surface, at first suppress soil surface possibility damage to crops stem, leaf, flower, the sprouting of disease fungal spore really and the growth of mycelia, reduce the dynamics that infects of disease fungi.Biocontrol fungi produces a large amount of spores and discharges, disperses in the field in the soil surface reproductive process, the part spore can land may be played a protective role by the position that the disease fungi encroaches in stem of plant stem of plant, leaf, flower, fruit; Biocontrol fungi can discharge a large amount of volatility antibiotic in growth, reproductive process, can suppress soil surface and stem of plant, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.This is of the present invention one big characteristics.
The effect of implementing the inventive method prevention root of the crop, basal part of stem fungal disease is sustainable more than two months; Anti-stem of plant, leaf, flower, fruit position fungal disease effect are sustainable more than 25 days.
Embodiment
Below specify the application process of biocontrol fungi agent of the present invention.
The application process of biocontrol fungi agent is that the biocontrol fungi agent that comprises the biocontrol fungi spore is spread fertilizer over the fields on the damp soil surface, make biocontrol fungi the soil surface layer fast, raised growth, produce spore.
Described biocontrol fungi spore is a kind of or two kinds combination or several the combination in the fungal spore kind (as conidium, chlamydospore, ascospore etc.).
Concrete may further comprise the steps: (1) is poured water in advance, is sprayed water or sprinkle water the soil of land for growing field crops, booth, greenhouse, cultivated plant such as potted plant, makes ground moistening; (2) the biocontrol fungi agent is evenly spread fertilizer over the fields on the damp soil surface.When by chance behind rain, promptly needn't pour water again or sprinkle water.
At the different stage of crop, should distinguish to some extent and spread fertilizer over the fields:
With after planting and before the seedling sowing, the biocontrol fungi agent is evenly spread fertilizer over the fields at soil surface before the A, planting seed;
B, seedling after planting and the plant growth stage evenly spread fertilizer over the fields soil surface between seedling to the biocontrol fungi agent, avoid being sprinkling upon the blade face and directly are sprinkling upon the foundation place;
C, to potted plant, surface at basin limit soil is evenly spread fertilizer over the fields in the biocontrol fungi agent.
The biocontrol fungi agent is sprinkling upon the blade face can be influenced it and expand numerous effect, is sprinkling upon the foundation place crop of can burning in a large number, therefore will avoid spreading fertilizer over the fields like this.
Spreading fertilizer over the fields the biocontrol fungi agent should carry out in the afternoon.
The amount of spreading fertilizer over the fields of biocontrol fungi agent is that per hectare is with 22.5 kilograms～150 kilograms.
The biocontrol fungi agent is the homogeneous mixture of agent of biological and ecological methods to prevent plant disease, pests, and erosion fungal spore and nutrition base, sterilizes 30 ± 5 minutes under 120 ± 5 ℃ of conditions before the nutrition base mixes with the agent of biocontrol fungi spore.The agent of described biocontrol fungi spore is for cultivating the solid or liquid or the solid and the mixtures of liquids that contain a large amount of biocontrol fungi spores and a small amount of viable bacteria filament of gained to biocontrol fungi.The biocontrol fungi spore agent that will comprise the biocontrol fungi spore becomes biocontrol fungi agent of the present invention with the even combined group of nutrition base.Its advantage is: carry the nutrition base.The advantage that carries the nutrition base has two aspects.The first, supply with biocontrol fungi with abundant nutrition make its mycelia fast, raised growth, grow in soil surely, and produce a large amount of spores, make the spore amount of supplying with the field can enlarge tens of times to hundreds of times.It two is, when the biocontrol fungi agent has under the situation of part spore inactivation in transportation with in storing, as long as the nutrition base that has the small part spore germination just can rely on to carry is quick, raised growth, product spore.
The proportioning of agent of biocontrol fungi spore and nutrition base is:
A, when the solid biocontrol fungi spore agent drying through cultivating gained is crushed into conidial powder, the percentage by weight of conidial powder is 25%～75%, all the other are the nutrition base of drying pulverizing;
B, when the liquid biocontrol fungi spore agent through cultivating gained is spore liquid, the percentage by weight of spore liquid is 30%～70%, all the other are the nutrition base of drying pulverizing;
C, when conidial powder and spore liquid are mixed into the agent of biocontrol fungi spore, the percentage by weight of conidial powder and spore liquid is 25%～75%, all the other are the nutrition base that drying is pulverized;
Wherein, but spore liquid and nutrition basigamy than preceding elimination moisture, the granularity of the nutrition base that drying is pulverized is below the 5mm.
The agent of described biocontrol fungi spore is as medium biocontrol fungi to be cultivated gained with following material:
A, grain (as rice, broomcorn millet, millet, wheat, bean, corn etc.) seed are or/and its by product; Perhaps
The branch of B, plant, leaf, root, flower or fruit, perhaps its mixture; Perhaps
The skin of C, animal, hair or meat, perhaps its mixture; Perhaps
Two kinds combination among three kinds of A, B, the C or several combination.
As required, can in medium, add mineral matter.
Cultivation, expansion to biocontrol fungi is numerous, processing method can be conventional solid culture, liquid culture or other cultural method, also can be that solid culture, the liquid cultivating method to aforementioned routine carries out improved cultural method.
Described nutrition base is:
A, grain (as rice, broomcorn millet, millet, wheat, bean, corn etc.) seed are or/and its by product; Perhaps
The branch of B, plant, leaf, root, flower or fruit, perhaps its mixture; Perhaps
The skin of C, animal, hair or meat, perhaps its mixture; Perhaps
Two kinds combination among three kinds of A, B, the C or several combination.
As required, can in the nutrition base, add mineral matter.
The advantage of the application process of biocontrol fungi agent:
The application process of one, former biocontrol fungi product is to mix that hundreds of times in soil spreads fertilizer over the fields or admix plow layer, plant plant hole with the centesimal ratios of number, and such application process recruitment is too big, and practical application is had any problem.
Implement the application process of this biocontrol fungi agent as long as spread fertilizer over the fields to moistening plow layer surface, method is simple, allows the user accept easily.
Two, former amount of application is excessive, and by plow layer, plant plant hole and mix and excute a law, every mu of the consumption of solid biological and ecological methods to prevent plant disease, pests, and erosion pulvis is more than double centner, and cost is higher.
The application process of implementing the agent of this biocontrol fungi only needs with 1.5 kilograms～10.0 kilograms for every mu, and cost alleviates tens of times.
It three is effect aspects, the biocontrol fungi conidial powder is mixed soil and is spread fertilizer over the fields, admixes plow layer, plants plant hole, method such as spray, be subjected to the influence of the antibacterial factor in the soil, the biological and ecological methods to prevent plant disease, pests, and erosion spore germination is inconsistent, be difficult to reach the effect that biocontrol fungi is generally grown comprehensively surely, add commodity biocontrol fungi conidial powder part inactivation in the storage transportation, influence protection effect.
The advantage of implementing the application process of this biocontrol fungi agent is: carry the nutrition base, and when the biocontrol fungi agent is sprinkling upon on the damp soil, the very fast moisture absorption of biocontrol fungi agent, under suitable temperature, endospore sprouting in 6 hours, mycelial growth just can grow into bacterium colony in 12 hours, and bacterium colony can produce spore in 24 hours.The field application process of biocontrol fungi agent was spread fertilizer over the fields in one week of back, and every gram can produce spore more than 1,000,000,000.These fresh and alive spores are with flow air between crop, are diffused into each corner of soil surface and stem of plant, leaf, flower, really, sprout growth and growing surely in suitable place.
In the said process, the hypha,hyphae of bacterium colony bottom can be grown in soil fast because of obtaining the abundant nutrition of nutrition base, grows in soil surely.
Implement this method and spread fertilizer over the fields the biocontrol fungi agent in soil surface, biocontrol fungi can obtain sufficient oxygen, and is not subjected to the influence of the antibacterial factor in the soil, and growth rate is than fast several times in soil, and this is big characteristics of this product.
The mechanism of implementing this method prevention crop root, basal part of stem fungal disease is, biocontrol fungi agent dispensing is grown fast, is bred behind the damp soil surface and grows surely in soil, suppress the sprouting of disease fungal spore in the soil and the growth of disease hypha,hyphae, thereby inhibition disease fungi is infected crop, reaches the purpose that the prevention crop fungal disease takes place.
Implement this method prevention stem of plant, leaf, flower if really the mechanism of fungus diseases is, the sprouting of the disease fungal spore that biocontrol fungi is grown at soil surface, breeding at first suppresses soil surface possibility damage to crops stem, leaf, flower, fruit and the growth of mycelia, the dynamics that infects of minimizing disease fungi.Biocontrol fungi produces a large amount of spores and discharges, disperses in the field in soil surface growth, reproductive process, the part spore can land may be played a protective role by the position that the disease fungi encroaches in stem of plant stem of plant, leaf, flower, fruit; Biocontrol fungi can discharge a large amount of volatility antibiotic in growth, reproductive process, can suppress soil surface and stem of plant, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.
The effect of implementing this method prevention root of the crop, basic stem fungal disease is sustainable more than two months; Prevention stem of plant, leaf, flower, fruit position fungal disease effect are sustainable more than 25 days.
Implement this method, enforcement is simple, cost is low, protection effect is obvious, and non agricultural chemical residuum, is specially adapted to the pollution-less agriculture field.
Implement the application process diseases prevention example of this biocontrol fungi agent:
The prevention of example one, vegetables, grey mould fruit rot of strawberry
The bacterial classification that this example uses is fungus Trichoderma Trichoderma harzianum two strain bacterial strains (numbering T25 and T96 certainly).
The prescription of conidial powder medium: wheat bran 600 grams, rapeseed cake 380 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 600 grams, rapeseed cake 380 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 5mm.
The weight proportion of conidial powder and nutrition base is 25:75, is 120 kilograms of per hectares at the morbidity season crop premorbid amount of application first time, and executing the applied once amount again in 1 month at interval is 45 kilograms of per hectares, and this example is executed secondary altogether, and protection effect reaches 91%.
The prevention of example two, cotton seedling blight (claiming the cotton seedling blight again)
The bacterial classification that this example uses is fungus Trichoderma Trichoderma harzianum one a strain bacterial classification (numbering T96 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 380 grams, corn flour 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 380 grams, rice meal 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.5mm.
The weight proportion of conidial powder and nutrition base is 30:70, and per hectare is executed 45 kilograms before seedling is sowed, and executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 93%.
The prevention of example three, peanut damping off
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum one strain bacterial classification (numbering T96 certainly) and Trichoderma viride one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 400 grams, corn stalk powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 400 grams, corn stalk powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder and nutrition base is 35:65, uses before sowing, and per hectare is executed 45 kilograms, executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 95%.
The prevention of example four, pimento and Phytophthora capsici disease
The bacterial classification that this example uses is fungus Trichoderma Trichoderma viride one a strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 400 grams, assorted leaf powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 400 grams, assorted leaf powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder and nutrition base is 40:60, uses after sowing, and per hectare is executed 45 kilograms, executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 90%.
The prevention of example five, jasmine southern blight
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum two strain bacterial classifications (numbering T25, T96 certainly) and Trichoderma viride one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 580 grams, rapeseed cake 400 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The prescription of spore liquid medium: potato 200 grams, sucrose 20 grams, potassium dihydrogen phosphate 1.5 grams, magnesium sulfate 0.5 gram add 1000 milliliters in water.The nutrition based formulas: wheat bran 580 grams, rapeseed cake 400 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder, spore liquid and nutrition base is 25:20:55, and 45 kilograms of per hectare consumptions are used at morbidity season crop premorbid, executes once in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 94%.
The prevention of example six, orchid fusarium wilt (claiming stem rot again)
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum two strain bacterial classifications (numbering T25, T96 certainly) and Trichoderma viride one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 430 grams, corn flour 50 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The prescription of spore liquid medium: potato 200 grams, sucrose 20 grams, potassium dihydrogen phosphate 1.5 grams, magnesium sulfate 0.5 gram add 1000 milliliters in water.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 430 grams, corn flour 50 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 3.0mm.
The weight proportion of conidial powder, spore liquid and nutrition base is 50:20:30, and 60 kilograms of per hectare consumptions are used at morbidity season crop premorbid, executes once in 45 days at interval, and this example is Shi Sici altogether, and protection effect reaches 96%.
The prevention of example seven, sclerotinia rot of colza
The bacterial classification that this example uses is Gliocladium fungi one a strain bacterial strain (numbering G21 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 380 grams, rice meal 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 600 grams, rapeseed cake 380 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.5mm.
The weight proportion of conidial powder and nutrition base is 35:65,, use according to the inventive method at morbidity season crop premorbid, the per hectare consumption is 60 kilograms for the first time, executes once 30 kilograms of per hectare consumptions in 1 month at interval later on, this example is executed 3 times altogether, and protection effect reaches 89%.
The prevention of example eight, eggplant damping off and southern blight
The bacterial classification that this example uses is Coniothyrium fungi one a strain bacterial strain (numbering C36 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 380 grams, corn stalk powder 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 380 grams, corn stalk powder 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.5mm.
The weight proportion of conidial powder and nutrition base is 30:70, use according to the inventive method at morbidity season crop premorbid, for the first time the per hectare consumption is 60 kilograms, executes 30 kilograms of per hectare consumptions later at interval in 1 month, this example is executed 3 times altogether, and protection effect reaches 92%.
The prevention of example nine, tomato wilt
The bacterial classification that this example uses is Chaetomium fungi one a strain bacterial strain (numbering C16 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 380 grams, corn flour 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 380 grams, corn flour 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 3.0mm.
The weight proportion of conidial powder and nutrition base is 35:65, use according to the inventive method at morbidity season crop premorbid, for the first time the per hectare consumption is 60 kilograms, executes 30 kilograms of per hectare consumptions later at interval in 2 months, this example is executed 2 times altogether, and protection effect reaches 85%.
Remarks:
Expand numerous, i.e. expanding propagation; Field proliferating is promptly carried out expanding propagation in the field.
The expansion of biocontrol fungi in the past is numerous all to be finished in factory or laboratory, and the biocontrol fungi spore preparation that people will expand after numerous is directly discharged the field, and to reach the purpose of preventing and treating crop pest, its application process mainly contains two kinds:
One is the biocontrol fungi spore preparation is mixed that soil spreads fertilizer over the fields or directly to admix soil or admix kind of a plant hole, makes the biocontrol fungi spore sprout in soil, grow and grow in soil surely.
Its two, be with the biocontrol fungi spore preparation with dusting or converting water sprays, the biocontrol fungi spore is sprayed directly on to stem, leaf, flower, the fruit position of plant, make that it is sprouted at these positions, growth and growing surely.
Biocontrol epiphyte field propagation agent then is to form with biocontrol fungi spore and nutrition base, will expand numerous dose of dispensing in the field, and biocontrol fungi is grown, bred in the field, enlarges the spore amount.The spore that these expanding propagation are come out can be diffused in the soil that does not spread spore growth and grow surely, reaches the purpose of comprehensive protection.The spore that expanding propagation is come out can discharge, disperse, and the part spore can land may be sprouted, grow, grow surely in stem of plant, leaf, flower, fruit by the position that the disease fungi encroaches on, and plays a protective role.Biocontrol fungi discharges a large amount of volatility antibiotic in field growing, reproductive process, can suppress soil surface and stem of plant, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.
Claims (10)
1, the application process of biocontrol fungi agent is characterized in that the biocontrol fungi agent that comprises the biocontrol fungi spore is spread fertilizer over the fields on the damp soil surface, and the biocontrol fungi of leaving expands numerous naturally.
2, the application process of biocontrol fungi agent according to claim 1 is characterized in that may further comprise the steps:
(1) soil of land for growing field crops, booth, greenhouse, cultivated plant such as potted plant is poured water in advance, sprayed water or sprinkles water, make ground moistening;
(2) the biocontrol fungi agent is evenly spread fertilizer over the fields on the damp soil surface.
3, the application process of biocontrol fungi agent according to claim 1 and 2 is characterized in that:
With after planting and before the seedling sowing, the biocontrol fungi agent is evenly spread fertilizer over the fields at soil surface before the A, planting seed;
B, seedling after planting and the plant growth stage evenly spread fertilizer over the fields soil surface between seedling to the biocontrol fungi agent, avoid being sprinkling upon the blade face and directly are sprinkling upon the foundation place;
C, to potted plant, surface at basin limit soil is evenly spread fertilizer over the fields in the biocontrol fungi agent.
4, the application process of biocontrol fungi agent according to claim 1 is characterized in that 22.5 kilograms～150 kilograms of biocontrol fungi agent of per hectare.
5, the application process of biocontrol fungi agent according to claim 1, it is characterized in that described biocontrol fungi agent is the homogeneous mixture of agent of biological and ecological methods to prevent plant disease, pests, and erosion fungal spore and nutrition base, the agent of described biocontrol fungi spore is for cultivating the solid or liquid or the solid and the mixtures of liquids that contain a large amount of biocontrol fungi spores and a small amount of viable bacteria filament of gained to biocontrol fungi.
6, the application process of biocontrol fungi agent according to claim 5 is characterized in that the proportioning of agent of described biocontrol fungi spore and nutrition base is:
A, when the solid fungal spore agent drying through cultivating gained is crushed into conidial powder, the percentage by weight of conidial powder is 25%～75%, all the other are the nutrition base of drying pulverizing;
B, when the liquid fungal spore agent through cultivating gained is spore liquid, the percentage by weight of spore liquid is 30%～70%, all the other are the nutrition base of drying pulverizing;
C, when conidial powder and spore liquid are mixed into the agent of biocontrol fungi spore, the percentage by weight of conidial powder and spore liquid is 25%～75%, all the other are the nutrition base that drying is pulverized.
7, the application process of biocontrol fungi agent according to claim 6 is characterized in that described spore liquid and nutrition basigamy are than preceding elimination moisture.
8, the application process of biocontrol fungi agent according to claim 6 is characterized in that the granularity of the nutrition base that described drying is pulverized is below the 5mm.
9, the application process of biocontrol fungi agent according to claim 1 is characterized in that described biocontrol fungi spore is a kind of or two kinds combination or several the combination in the fungal spore kind.
10, the application process of biocontrol fungi agent according to claim 1, it is characterized in that spreading fertilizer over the fields the biocontrol fungi agent should carry out in the afternoon.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100958753A CN101480156A (en) | 2009-02-16 | 2009-02-16 | Method for using fungal biocontrol agents |
| CN200910164188A CN101642036A (en) | 2009-02-16 | 2009-08-05 | Application process of fungus biocontrol agent |
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| CNA2009100958753A CN101480156A (en) | 2009-02-16 | 2009-02-16 | Method for using fungal biocontrol agents |
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| CN101480156A true CN101480156A (en) | 2009-07-15 |
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| CNA2009100958753A Pending CN101480156A (en) | 2009-02-16 | 2009-02-16 | Method for using fungal biocontrol agents |
| CN200910164188A Pending CN101642036A (en) | 2009-02-16 | 2009-08-05 | Application process of fungus biocontrol agent |
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| CN200910164188A Pending CN101642036A (en) | 2009-02-16 | 2009-08-05 | Application process of fungus biocontrol agent |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103875717A (en) * | 2014-04-02 | 2014-06-25 | 湖南省植物保护研究所 | Method for promoting orchid to grow and preventing diseases |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103004546A (en) * | 2011-09-22 | 2013-04-03 | 庞占琴 | Nectarine planting method |
| CN105660256A (en) * | 2015-11-18 | 2016-06-15 | 昆明保腾生化技术有限公司 | Biological control method for tomato root knot nematodes and biocontrol agent thereof |
| CN105660255A (en) * | 2015-11-18 | 2016-06-15 | 昆明保腾生化技术有限公司 | Biological control method for tobacco rot-not nematodes diseases and biocontrol agent thereof |
| CN105660254A (en) * | 2015-11-18 | 2016-06-15 | 昆明保腾生化技术有限公司 | Biological control method and biocontrol agent for banana vasicular wilt |
-
2009
- 2009-02-16 CN CNA2009100958753A patent/CN101480156A/en active Pending
- 2009-08-05 CN CN200910164188A patent/CN101642036A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103875717A (en) * | 2014-04-02 | 2014-06-25 | 湖南省植物保护研究所 | Method for promoting orchid to grow and preventing diseases |
| CN103875717B (en) * | 2014-04-02 | 2015-05-20 | 湖南省植物保护研究所 | Method for promoting orchid to grow and preventing diseases |
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| CN101642036A (en) | 2010-02-10 |
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