CN101595816A - Application process for trichodermin preventer - Google Patents
Application process for trichodermin preventer Download PDFInfo
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- CN101595816A CN101595816A CNA2009101011765A CN200910101176A CN101595816A CN 101595816 A CN101595816 A CN 101595816A CN A2009101011765 A CNA2009101011765 A CN A2009101011765A CN 200910101176 A CN200910101176 A CN 200910101176A CN 101595816 A CN101595816 A CN 101595816A
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Abstract
The invention discloses a kind of application process for trichodermin preventer, belong to application of microorganism.The application process recruitment of existing trichoderma as biological control product is big, spore germination is poor, grows undesirable surely.Application process for trichodermin preventer of the present invention is that the trichoderma as biological control agent that comprises wooden mould fungal spore agent and nutrition base is spread fertilizer over the fields on the damp soil surface, makes wooden mould fungi grow, expand numerous fast.The enforcement of the inventive method: pour water in advance, spray water, watering or drip irrigation, infiltrating irrigation, make ground moistening, spread fertilizer over the fields trichoderma as biological control agent to the damp soil surface then, method is simple, and recruitment economizes, and allows the user accept easily.Implement this method and use trichoderma as biological control agent, supply with wooden mould mycotrophy at soil surface by the nutrition base, make wooden mould fungi grow, expand numerous fast, not only can prevent the fungal disease of plant root and basal part of stem, can prevent the fungal disease at axis, leaf, flower, fruit position simultaneously.
Description
Technical field
The invention belongs to the microorganism application, be specifically related to application process for trichodermin preventer.
Background technology
Now, fungal diseases of plants has restricted high yield, stable yields and the high-quality of crop.The raising day by day that pollution-free, nuisanceless green organic food is required along with people, expert, scholar are devoted to not have the residual biological control research of chemical pesticide and do one's utmost, and some have antagonism disease fungi fungus Trichoderma function, that can be used for biological control to have filtered out, koning trichoderma mould as Trichoderma harzianum, Trichoderma viride, long shoot wood, mould, the yellow green trichoderma of hook-shaped wood and tangerine green trichoderma etc.What develop in recent years, register both at home and abroad is that the trichoderma as biological control agent quantity of biological control bacterial classification also has certain scale with the mould fungi of wood, but fails so far to be widely used, and studying carefully in reason is that application process haves much room for improvement.
Existing application process mainly is to mix soil to spread fertilizer over the fields, directly admix plow layer, plant plant hole, irritate root or spray.Such application process not only recruitment is big, and because of being subjected to the influence of the antibacterial factor and residue of pesticide in the soil, and spore germination, mycelial growth are poor, grow undesirable surely.Spray in this way, limit to because of being subjected to weather, environmental influence and spraying technology own, wooden mould fungal spore will be sprouted, grow surely more difficult at stem, leaf, flower, the fruit position of crop.Because the spore of wooden mould fungi becomes current shorter, in the transportation of commodity preparation with in storing the part inactivation is arranged, under the situation that the spore amount of living reduces, above-mentioned application process influences sprouting, the growth of wooden mould fungi especially and grows surely.
Summary of the invention
The technical assignment of the technical problem to be solved in the present invention and proposition be overcome trichoderma as biological control agent product that prior art exists use that recruitment is big, the sprouting of spore is poor, grow, grow shortcomings such as undesirable surely, a kind of application process for trichodermin preventer is provided.For this reason, below the present invention adopts
Technical scheme:
Application process for trichodermin preventer is characterized in that the trichoderma as biological control agent that comprises wooden mould fungal spore agent and nutrition base is spread fertilizer over the fields on the damp soil material of planting of various materials (or for) surface, makes that wooden mould fungi is quick, raised growth, expansion be numerous.
The invention has the beneficial effects as follows:
One, the former method of using trichoderma as biological control agent are to mix native hundreds of times to spread fertilizer over the fields or admix plow layer, plant plant hole with the centesimal ratio of number, and such application process recruitment is big, and practical application is had any problem.
Of the present inventionly use trichoderma as biological control agent method as long as spread fertilizer over the fields to the damp soil material of planting of various materials (or for) surface, method is simple, and recruitment province allows the user accept easily.
Two, former amount of application is excessive, and by plow layer, plant plant hole and mix and excute a law, every mu of the consumption of solid powder is more than double centner, and cost is higher.
Use the present invention, every mu only needs with 1.5 kilograms to 10 kilograms, and cost alleviates tens of times.
Three, effect aspect, trichoderma as biological control agent is mixed soil and is spread fertilizer over the fields, admixes plow layer, plants plant hole, method such as irritate root or spray, be subjected to the influence of the antibacterial factor in the soil, spore germination is poor, be difficult to reach general effect of comprehensively growing surely, adding commodity preparation wooden mould spore in the storage transportation has the part inactivation, influences protection effect especially.
Implementing advantage of the present invention is, by the field proliferating effect, promptly prevent and treat agent and be sprinkling upon damp soil (or being the material of planting of various materials) surface when trichoderma as biological, the very fast moisture absorption of trichoderma as biological control agent, under suitable temperature, endospore sprouting in 6 hours, mycelial growth just can grow into bacterium colony in 12 hours, and bacterium colony can produce spore in 24 hours.Trichoderma as biological control agent was spread fertilizer over the fields in one week of back, and every gram can produce spore more than 1,000,000,000.These fresh and alive spores are with flow air between plant, are diffused into each corner of soil surface and axis, leaf, flower, really, sprout in suitable place, growth and growing surely.
In the said process, Trichoderma falls the wooden mould hypha,hyphae of portion because of obtaining the abundant nutrition of nutrition base, can grow in soil fast, grows in soil surely.
Trichoderma as biological control agent dispensing can obtain sufficient oxygen, and not be subjected to the influence of the antibacterial factor in the soil on soil (or being the material of planting of various materials) surface, and growth rate is than fast several times in soil, and this is of the present invention one big characteristics.
Implementing the present invention prevents the mechanism of plant root, basal part of stem fungal disease to be, trichoderma as biological control agent is discharged in soil (or being the material of planting of various materials) back, surface and is grown fast, breeds and grow surely in soil, suppress the sprouting of disease fungal spore in the soil (or being the material of planting of various materials) and the growth of disease hypha,hyphae, inhibition disease fungi is infected plant, reaches the purpose that the prevention fungal diseases of plants takes place.
Implement the present invention and prevent axis, leaf, flower if really the mechanism of fungus diseases is, the mould fungi of wood grows fast, breeds on soil (or being the material of planting of various materials) surface, at first suppress to endanger axis, leaf, flower, the sprouting of disease fungal spore really and the growth of mycelia in the soil (or being the material of planting of various materials), reduce the dynamics that infects of disease fungi.The mould fungi of wood produces a large amount of spores and discharges, disperses in the field in the surperficial reproductive process of soil (or being the material of planting of various materials), the part spore can land may be played a protective role by the position that the disease fungi encroaches in axis, leaf, flower, fruit; The mould fungi of wood can discharge a large amount of volatility antibiotic in growth, reproductive process, can suppress in the soil (or being the material of planting of various materials) and axis, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.This is another big characteristics of the present invention.
The effect of implementing the inventive method prevention plant roots, basal part of stem fungal disease is sustainable more than two months; Prevention axis, leaf, flower, fruit position fungal disease effect are sustainable more than 25 days.
Embodiment
Below specify application process for trichodermin preventer of the present invention.
Application process for trichodermin preventer, be that the trichoderma as biological control agent that will comprise wooden mould fungal spore agent and nutrition base is spread fertilizer over the fields on damp soil (or being the material of planting of various materials) surface, superficial layer is quick, raised growth, produces spore at soil (or being the material of planting of various materials) to make wooden mould fungi.
Concrete may further comprise the steps: (1) to the soil of land for growing field crops, booth, greenhouse, cultivated plant such as the potted plant material of planting of various materials (or for) pour water in advance, spray water, watering or drip irrigation, infiltrating irrigation, make soil (or being the material of planting of various materials) moistening; Descend as the moistening situation of soil behind rain (or for various materials plant material), promptly needn't pour water again, spray water, watering or drip irrigation, infiltrating irrigation.
(2) trichoderma as biological control agent is evenly spread fertilizer over the fields on damp soil (or being the material of planting of various materials) surface.
At the different stage of crop, should distinguish to some extent and spread fertilizer over the fields:
With after planting and before the seedling sowing, trichoderma as biological control agent is evenly spread fertilizer over the fields on the soil material of planting of various materials (or for) surface before the A, planting seed;
B, seedling after planting and the plant growing stage evenly spread fertilizer over the fields soil between the plant material of planting of various materials (or for) surface to trichoderma as biological control agent, avoid being sprinkling upon the blade face and directly are sprinkling upon the foundation place;
C, to potted plant, surface at the basin limit soil material of planting of various materials (or for) is evenly spread fertilizer over the fields in trichoderma as biological control agent.
Trichoderma as biological control agent is sprinkling upon the blade face can influence its effect, is sprinkling upon the foundation place crop of can burning in a large number, therefore will avoid spreading fertilizer over the fields like this.
Spreading fertilizer over the fields trichoderma as biological control agent should be afternoon to carrying out at dusk.To before the planting seed with after planting and before the seedling sowing, the less field of plant spreads fertilizer over the fields trichoderma as biological control agent and should carry out behind not direct projection of sun soil face.
The amount of application of trichoderma as biological control agent is that per hectare is with 22.5 kilograms～150 kilograms.
Trichoderma as biological control agent of the present invention is the homogeneous mixture of wooden mould fungal spore agent and nutrition base.The mould fungal spore agent of described wood is the solid or liquid or solid and the mixtures of liquids that contain the mould fungal spore of a large amount of wood and a small amount of wooden mould fungi viable bacteria filament to the mould fungal culture gained of wood.Described nutrition base is for growing, produce the nutriment that spore provides carbon, nitrogen and mineral matter and trace element fast for the mould fungi of wood.Before mixing with wooden mould fungal spore agent, the nutrition base under 120 ± 5 ℃ of conditions, sterilized 30 ± 5 minutes.
The advantage of application process for trichodermin preventer:
The application process of one, former wooden mould fungus biocontrol product is to mix that hundreds of times in soil spreads fertilizer over the fields or admix plow layer, plant plant hole with the centesimal ratios of number, and such application process recruitment is big, and practical application is had any problem.
Implement this application process for trichodermin preventer as long as spread fertilizer over the fields to moistening plow layer surface, method is simple, allows the user accept easily.
Two, former amount of application is excessive, and by plow layer, plant plant hole and mix and excute a law, every mu of the consumption of solid biological and ecological methods to prevent plant disease, pests, and erosion pulvis is more than double centner, and cost is higher.
Implementing this application process for trichodermin preventer only needs with 1.5 kilograms～10.0 kilograms for every mu, and cost alleviates tens of times.
It three is effect aspects, the biocontrol fungi conidial powder is mixed soil and is spread fertilizer over the fields, admixes plow layer, plants plant hole, method such as irritate root or spray, be subjected to the influence of the antibacterial factor in the soil, the mould spore germination of wood is poor, be difficult to reach the effect that wooden mould fungi is generally grown comprehensively surely, add commodity preparation part fungal spore inactivation in the storage transportation, influence protection effect.
The advantage of implementing this application process for trichodermin preventer is: when trichoderma as biological control agent is sprinkling upon the damp soil surface, the very fast moisture absorption of trichoderma as biological control agent, under suitable temperature, endospore sprouting in 6 hours, mycelial growth, just can grow into bacterium colony in 12 hours, bacterium colony can produce spore in 24 hours.Trichoderma as biological control agent was spread fertilizer over the fields behind the field in the week, and every gram can produce spore more than 1,000,000,000.These fresh and alive spores are with flow air between crop, are diffused into each corner of soil surface and stem of plant, leaf, flower, really, sprout growth and growing surely in suitable place.
In the said process, the hypha,hyphae of bacterium colony bottom can be grown in soil fast because of obtaining the abundant nutrition of nutrition base, grows in soil surely.
Implement this method and spread fertilizer over the fields trichoderma as biological control agent in soil surface, wooden mould fungi can obtain sufficient oxygen, and is not subjected to the influence of the antibacterial factor in the soil, and growth rate is than fast several times in soil, and this is of the present invention one big characteristics.
The mechanism of implementing this method prevention plant root, basal part of stem fungal disease is, trichoderma as biological control agent dispensing is grown fast, is bred behind the damp soil surface and grows surely in soil, suppress the sprouting of disease fungal spore in the soil and the growth of disease hypha,hyphae, thereby inhibition disease fungi is infected crop, reaches the purpose that the prevention fungal diseases of plants takes place.
Implement this method prevention axis, leaf, flower if really the mechanism of fungus diseases is, the mould fungi of wood grows at soil surface, breeding at first suppresses soil surface and may endanger axis, leaf, flower, the sprouting of disease fungal spore really and the growth of mycelia, reduces the dynamics that infects of disease fungi.The mould fungi of wood produces a large amount of spores and discharges, disperses in the field in soil surface growth, reproductive process, the part spore can land may be played a protective role by the position that the disease fungi encroaches in axis stem of plant, leaf, flower, fruit; The mould fungi of wood can discharge a large amount of volatility antibiotic in growth, reproductive process, can suppress soil surface and axis, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.
Implement this method, use simple, cost is low, protection effect is obvious, and non agricultural chemical residuum is specially adapted to the pollution-less agriculture field.
Implement the diseases prevention example of this application process for trichodermin preventer:
The prevention of example one, vegetables, grey mould fruit rot of strawberry
The bacterial classification that this example uses is fungus Trichoderma Trichoderma harzianum two strain bacterial strains (numbering T25 and T96 certainly).
The prescription of conidial powder medium: wheat bran 600 grams, rapeseed cake 380 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 600 grams, rapeseed cake 380 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 5mm.
The weight proportion of conidial powder and nutrition base is 25: 75, is 120 kilograms of per hectares at the morbidity season crop premorbid amount of application first time, and executing the applied once amount again in 1 month at interval is 45 kilograms of per hectares, and this example is executed secondary altogether, and protection effect reaches 91%.
The prevention of example two, cotton seedling blight (claiming the cotton seedling blight again)
The bacterial classification that this example uses is fungus Trichoderma Trichoderma harzianum one a strain bacterial classification (numbering T96 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 380 grams, corn flour 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 380 grams, rice meal 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.5mm.
The weight proportion of conidial powder and nutrition base is 30: 70, and per hectare is executed 45 kilograms before seedling is sowed, and executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 93%.
The prevention of example three, peanut damping off
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum one strain bacterial classification (numbering T96 certainly) and Trichoderma viride one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 400 grams, corn stalk powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 400 grams, corn stalk powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder and nutrition base is 35: 65, uses before sowing, and per hectare is executed 45 kilograms, executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 95%.
The prevention of example four, pimento and Phytophthora capsici disease
The bacterial classification that this example uses is fungus Trichoderma Trichoderma viride one a strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 400 grams, assorted leaf powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 400 grams, assorted leaf powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder and nutrition base is 40: 60, uses after sowing, and per hectare is executed 45 kilograms, executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 90%.
The prevention of example five, jasmine southern blight
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum two strain bacterial classifications (numbering T25, T96 certainly) and Trichoderma viride one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 580 grams, rapeseed cake 400 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The prescription of spore liquid medium: potato 200 grams, sucrose 20 grams, potassium dihydrogen phosphate 1.5 grams, magnesium sulfate 0.5 gram add 1000 milliliters in water.The nutrition based formulas: wheat bran 580 grams, rapeseed cake 400 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder, spore liquid and nutrition base is 25: 20: 55, and 45 kilograms of per hectare consumptions are used at morbidity season crop premorbid, executes once in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 94%.
The prevention of example six, orchid fusarium wilt (claiming stem rot again)
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum two strain bacterial classifications (numbering T25, T96 certainly) and Trichoderma viride one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 430 grams, corn flour 50 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The prescription of spore liquid medium: potato 200 grams, sucrose 20 grams, potassium dihydrogen phosphate 1.5 grams, magnesium sulfate 0.5 gram add 1000 milliliters in water.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 430 grams, corn flour 50 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 3.0mm.
Claims (6)
1, application process for trichodermin preventer is characterized in that the trichoderma as biological control agent that comprises wooden mould fungal spore agent and nutrition base is spread fertilizer over the fields on damp soil (or being the material of planting of various materials) surface, makes wooden mould fungi grow, expand numerous fast.
2, application process for trichodermin preventer according to claim 1, its feature may further comprise the steps:
(1) to the soil of land for growing field crops, booth, greenhouse, cultivated plant such as the potted plant material of planting of various materials (or for) pour water in advance, spray water, watering or drip irrigation, infiltrating irrigation, make soil (or being the material of planting of various materials) moistening; Descend as the moistening situation of soil behind rain (or for various materials plant material), promptly needn't pour water again, spray water, watering or drip irrigation, infiltrating irrigation.
(2) trichoderma as biological control agent is evenly spread fertilizer over the fields on damp soil (or being the material of planting of various materials) surface.
3, application process for trichodermin preventer according to claim 1 and 2 is characterized in that:
With after planting and before the seedling sowing, trichoderma as biological control agent is evenly spread fertilizer over the fields on the soil material of planting of various materials (or for) surface before the A, planting seed;
B, seedling after planting and the plant growing stage evenly spread fertilizer over the fields soil between the plant material of planting of various materials (or for) surface to trichoderma as biological control agent, avoid being sprinkling upon the blade face and directly are sprinkling upon the foundation place;
C, to the using of pot plant, should prevent and treat agent to trichoderma as biological and evenly spread fertilizer over the fields surface at the basin limit soil material of planting of various materials (or for).
4, trichoderma as biological control agent according to claim 1 is characterized in that described trichoderma as biological control agent is the homogeneous mixture of wooden mould fungal spore agent and nutrition base; The mould fungal spore agent of described wood is the solid or liquid or solid and the mixtures of liquids that contain the mould fungal spore of a large amount of wood and a small amount of wooden mould fungi viable bacteria filament to the mould fungal culture gained of wood.Described nutrition base is for growing, produce the nutriment that spore provides carbon, nitrogen and mineral matter and trace element fast for the mould fungi of wood.
5, trichoderma as biological according to claim 1 control agent is characterized in that the mould fungal spore of described wood is the combination of a kind of in the conidium of fungus Trichoderma or the chlamydospore or two kinds.
6, application process for trichodermin preventer according to claim 1, it is characterized in that spreading fertilizer over the fields trichoderma as biological control agent should be afternoon to carrying out at dusk.
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| CNA2009101011765A CN101595816A (en) | 2009-07-28 | 2009-07-28 | Application process for trichodermin preventer |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102219602A (en) * | 2011-04-22 | 2011-10-19 | 浙江科技学院 | Novel biocontrol and plant vigor complexing agent and preparation method thereof |
| CN102726256A (en) * | 2011-04-07 | 2012-10-17 | 何松庆 | New application method of trichoderma biocontrol agent |
| CN103004546A (en) * | 2011-09-22 | 2013-04-03 | 庞占琴 | Nectarine planting method |
| CN103329720A (en) * | 2013-07-10 | 2013-10-02 | 山东大学 | Application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion |
| CN103875717A (en) * | 2014-04-02 | 2014-06-25 | 湖南省植物保护研究所 | Method for promoting orchid to grow and preventing diseases |
-
2009
- 2009-07-28 CN CNA2009101011765A patent/CN101595816A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102726256A (en) * | 2011-04-07 | 2012-10-17 | 何松庆 | New application method of trichoderma biocontrol agent |
| CN102219602A (en) * | 2011-04-22 | 2011-10-19 | 浙江科技学院 | Novel biocontrol and plant vigor complexing agent and preparation method thereof |
| CN102219602B (en) * | 2011-04-22 | 2013-10-16 | 浙江科技学院 | Novel biocontrol and plant vigor complexing agent and preparation method thereof |
| CN103004546A (en) * | 2011-09-22 | 2013-04-03 | 庞占琴 | Nectarine planting method |
| CN103329720A (en) * | 2013-07-10 | 2013-10-02 | 山东大学 | Application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion |
| CN103329720B (en) * | 2013-07-10 | 2015-06-17 | 山东大学 | Application of trichoderma pseudokoningii SMF2 bacterial strains in plant growth promotion |
| CN103875717A (en) * | 2014-04-02 | 2014-06-25 | 湖南省植物保护研究所 | Method for promoting orchid to grow and preventing diseases |
| CN103875717B (en) * | 2014-04-02 | 2015-05-20 | 湖南省植物保护研究所 | Method for promoting orchid to grow and preventing diseases |
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Open date: 20091209 |