CN101449708B - Infant milk powder added with biostime and preparation method thereof - Google Patents
Infant milk powder added with biostime and preparation method thereof Download PDFInfo
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- CN101449708B CN101449708B CN2008102498088A CN200810249808A CN101449708B CN 101449708 B CN101449708 B CN 101449708B CN 2008102498088 A CN2008102498088 A CN 2008102498088A CN 200810249808 A CN200810249808 A CN 200810249808A CN 101449708 B CN101449708 B CN 101449708B
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Abstract
The invention discloses an infant milk power added with synbiotics, comprising the following components: fresh milk 40-68 gram; demineralized whey powder 15-34 gram; granulated sugar3-6 graml; vegetable oil 4-8 gram; compound prebiotics 0.4-20 gram; compound probiotic 1.0*10<5>-1.0*10<11> cfu. The inventive infant milk power can be ysed for building advantages of beneficial bacterium when a newborn infant starts colony valuation. Through adding probiotic, the number of probiotic is advanced to increase in a short time, through adding prebiotics having selectively increasing effect on probiotic, the added probiotic is performed with fast field setting, thereby maintaining advantages of probiotic in intestinal flora. The invention also markedly relieves diarrhoetic symptoms, and has definite treatment effect on diarrhea caused by rotavirus.
Description
Technical field
The present invention relates to a kind of add symphysis unit be suitable for milk powder of infant and preparation method thereof, belong to technical field of processing dairy products.
Background technology
As everyone knows, the baby is from being born by 3 years old, it is the most important stage of growing in life, really all babies being used breast milk is best nurture mode, yet often exist owing to breast milk not enough or other reason can not breastfeeding situation in the reality, at this moment just can only select high-quality baby formula milk powder.Existing baby formula milk powder is a lot, yet for the infant better grows, need continually develop new formula milk.
The life that breeds in mother uterus is integral asepsis, yet is born back first day, just can find the existence of bacterium in baby's ight soil, and being born back three days total bacteria counts can be up to 10
11Cfu/g is so the activity of bacterium in human body is very noticeable.
According to the interaction property of gut flora, enteric bacteria can be divided into beneficial bacterium, harmful bacterium and two-way bacterium three major types to body.Probio has the gut flora of improvement structure, suppresses pathogen, eliminates carcinogen, improves the immunity of organism factor, reduces physiological functions such as cholesterol.Usually, flora in the enteron aisle is to maintain certain balance, yet, situations such as antibiotic, diarrhoea constipation are drunk, are used in disadvantaged group (the elderly, infant), irregular, the frequent smoking of living and diet, the quantity of probio is destroyed, thereby need take measures, recover the superiority of beneficial bacterium.
Prebiotics is meant not the food component that can be digested by digestive juice, and it can enter large intestine, is absorbed by beneficial bacterium specific in the large intestine, promotes the growth of beneficial bacterium, thereby promotes human beings'health.Present prebiotics typically refers to compound sugar, mainly contains soyabean oligosaccharides, FOS, galactooligosaccharide, xylo-oligosaccharide, inulin etc.
Symphysis unit becomes Synbiotics again, is meant the combination preparation of probio and prebiotics, both can bring into play the physiological bacterial activity of probio, optionally increases the quantity of probio again fast, makes the effect of probio more remarkable and lasting.
The seventies in 20th century, Australian scientist has found very easily to cause " rotavirus " of infantile diarrhea.In China, children below 5 years old on average suffer from diarrhoea 11 times for each person every year, and wherein the infantile diarrhea that is caused by rotavirus is the most common, also the most serious.Annual nearly 900,000 infants in the whole world die from rotavirus infection diarrhoea.It is reported, the intestinal flora imbalance is the one of the main reasons that causes diarrhoea, and by observing, breastfeeding infantile diarrhea incidence is starkly lower than the infant of NBF, its main cause be exactly in breastfeeding infant's enteron aisle the quantity of Bifidobacterium more than the infant of NBF, thereby alleviated the infringement of rotavirus to body.Therefore, can predict, be chosen in the baby milk powder add an amount of, effectively probio will obviously alleviate the infringement of rotavirus to the infant.
No matter in sum, provide a kind of baby milk powder that adds symphysis unit, be to recovering, keep the superiority of profitable strain in the enteron aisle, still to the prevention rotavirus, all having important function and significance.In the prior art, add prebiotics or probio in the dairy produce bibliographical information though have, its effect is all general, and specific aim is not strong.
Summary of the invention
At above-mentioned prior art, the invention provides a kind of baby milk powder that adds symphysis unit and preparation method thereof.
A kind of baby milk powder that adds symphysis unit comprises following component: fresh milk, 40~68 grams; Desalted whey powder, 15~37 grams; Granulated sugar, 3~6 grams; Vegetable oil, 4~8 grams; Complex prebiotics, 0.4~20 gram; Compound probiotic 1.0 * 10
5~1.0 * 10
14Cfu.
Described fresh milk can be milk, goat milk; The form of fresh milk can be a liquid milk, also can be the milk powder that is directly dusted and obtained by liquid milk for convenience of transportation.The preferred peanut oil of described vegetable oil, olive oil, corn wet goods are rich in the vegetable oil of unrighted acid.
The baby milk powder of described interpolation symphysis unit also comprises following component: B B-complex, 0.023 gram; Composite mineral matter, 0.5 gram; Nucleotides, 0.05 gram; Taurine, 0.04 gram; DHA, 0.15 gram; Arachidonic acid, 0.1 gram; Lecithin, 0.2 gram; Beta carotene, 0.14 gram.
The content of described complex prebiotics is 2~10 grams.
Described complex prebiotics is a kind of or several arbitrary proportions combination in oligoisomaltose, FOS, xylo-oligosaccharide, galactooligosaccharide, chitosan oligomer, soyabean oligosaccharides, inulin, the milk ketose; The preferred combination of compound sugar more than three kinds.
Described compound probiotic is a kind of or several arbitrary proportions combination in bifidobacterium strains, lactobacillus strains, streptococcus bacterial strain, lactococcus bacterial strain, the Bacillus strain.
Described compound probiotic is the combination of bifidobacterium strains and lactobacillus strains.
Described bifidobacterium strains is bifidobacterium infantis, bifidobacterium adolescentis, bifidobacterium longum or bifidobacterium breve; Described lactobacillus strains is lactobacillus bulgaricus, lactobacillus acidophilus, lactobacillus thermophilus or Lactobacillus casei.
Described B B-complex is composed of the following components: vitamin A, 4 grams; Vitamin D, 2 grams; Vitamin E, 0.1 gram; Vitamin K
1, 0.035 gram; Cobastab
1, 0.6 gram; Cobastab
2, 0.8 gram; Cobastab
6, 0.35 gram; Cobastab
12, 0.015 gram; Vitamin C, 2 grams; Niacinamide usp, 8 grams; Folic acid, 0.08 gram; Calcium pantothenate, 5 grams; Biotin, 0.02 gram.
Described composite mineral matter is composed of the following components: ferrous lactate, 50 grams; Zinc lactate, 10 grams; Copper sulphate, 2 grams; Potassiumiodate, 0.13 gram; Calcium carbonate, 437.7 grams; Manganese sulfate, 0.17 gram.
A kind of preparation method who adds the baby milk powder of symphysis unit, step is: mix other components except that compound probiotic, B B-complex, get mixed solution, use high pressure homogenizer under 10~20MPa, to carry out homogeneous then, carry out spray-drying or freeze drying then, get powder product, add compound probiotic, B B-complex then, mixing, vacuum packaging promptly gets milk powder.
Milk powder of the present invention also can adopt other any suitable methods to process.
Baby formula milk powder of the present invention, when can be used for newborn infants and beginning the bacterium colony definite value, the foundation of beneficial bacterium advantage.By adding probio, increase that can short time internal stimulus beneficial bacterium quantity, and, can make the quick field planting of probio of interpolation by adding prebiotics to the selective value-added effect of probio, keep the advantage of beneficial bacterium at gut flora.
After the infant taking symphysis unit prescription milk powder of diarrhoea takes place, by observing, the obvious symptom of alleviating diarrhoea, and the diarrhoea that rotavirus causes had certain result of treatment.
At present, life usually needs usually to kill pathogenic bacteria in the body by taking antibiosis after being ill, still, after administration of antibiotics, because antibiotic does not have identity, can kill harmful bacterium in the enteron aisle and one of beneficial bacterium, the quantity of the flora in the enteron aisle is sharply reduced.In the infant of administration of antibiotics, take symphysis unit, can recover the balance of flora in the enteron aisle, and beneficial bacterium is had more obvious value-added effect, can strengthen beneficial bacterium superiority in the flora in enteron aisle.
The symphysis unit that adds among the present invention is the best of breed that draws by behind the verification experimental verification repeatedly for the organic assembling of compound probiotic and complex prebiotics, and its effect that plays not is the simple superposition of each single material, but organically combination.Also added B B-complex, composite mineral matter and other nutriment in the milk powder of the present invention, can provide comprehensive nutrition to the infant.Added the vegetable oil that peanut oil, olive oil, corn wet goods are rich in unrighted acid, can increase the content of unrighted acid and essential fatty acids linoleic in the product, infant's brain development has been had good effect.Added essential fatty acids such as DHA, ARA, also had nutriments such as lecithin, nucleotides, be beneficial to the growth of infant's brain cell, very big benefit has been arranged improving baby's memory.
The specific embodiment
The present invention is further illustrated with the test example below in conjunction with embodiment:
Embodiment 1: prepare a kind of baby milk powder that adds symphysis unit, wherein, the prescription of milk powder is as follows:
Milk (material of giving money as a gift) 47g
Desalted whey powder 36g
Granulated sugar 5g
Peanut oil 4g
Complex prebiotics 10g
Compound probiotic 1.0 * 10
8Cfu
B B-complex 0.023g
Composite mineral matter 0.5g
Nucleotides 0.05g
Taurine 0.04g
DHA 0.15g
ARA 0.1g
Choline 0.1g
Lecithin 0.2g
Beta carotene 0.14g.
Wherein, the composition weight proportioning of complex prebiotics is: oligoisomaltose: xylo-oligosaccharide: gossypose=10: 3: 5.
The composition weight proportioning of compound probiotic is: bifidobacterium infantis: bifidobacterium adolescentis: lactobacillus acidophilus=3: 2: 2.
The prescription of B B-complex is: vitamin A, 4g; Vitamin D, 2g; Vitamin E, 0.1g; Vitamin K
1, 0.035g; Cobastab
1, 0.6g; Cobastab
2, 0.8g; Cobastab
6, 0.35g; Cobastab
12, 0.015g; Vitamin C, 2g; Niacinamide usp, 8g; Folic acid, 0.08g; Calcium pantothenate, 5g; Biotin, 0.02g.
The prescription of composite mineral matter is: ferrous lactate, 50g; Zinc lactate, 10g; Copper sulphate, 2g; Potassiumiodate, 0.13g; Calcium carbonate, 437.7g; Manganese sulfate, 0.17g.
Preparation technology is: mix other components except that compound probiotic, B B-complex, get mixed solution, use high pressure homogenizer to carry out homogeneous then under 10~20MPa, carry out spray-drying then, get powder product, add compound probiotic, B B-complex then, mixing, vacuum packaging.
Embodiment 2: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is as follows:
Milk (material of giving money as a gift) 62g
Desalted whey powder 16g
Granulated sugar 6g
Olive oil 7g
Complex prebiotics 4g
Compound probiotic 1.0 * 10
12Cfu
B B-complex 0.023g
Composite mineral matter 0.5g
Nucleotides 0.05g
Taurine 0.04g
DHA 0.15g
ARA 0.1g
Choline 0.1g
Lecithin 0.2g
Beta carotene 0.14g.
Wherein, the composition weight proportioning of complex prebiotics is: soyabean oligosaccharides: xylo-oligosaccharide: gossypose=10: 2: 5.
The composition weight proportioning of compound probiotic is: bifidobacterium infantis: bifidobacterium adolescentis: lactobacillus acidophilus: lactobacillus thermophilus=3: 3: 2: 1.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 3: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is as follows:
Milk (material of giving money as a gift) 55g
Desalted whey powder 24g
Granulated sugar 5g
Corn oil 6g
Complex prebiotics 17g
Compound probiotic 1.0 * 10
10Cfu
B B-complex 0.023g
Composite mineral matter 0.5g
Nucleotides 0.05g
Taurine 0.04g
DHA 0.15g
ARA 0.1g
Choline 0.1g
Lecithin 0.2g
Beta carotene 0.14g.
Wherein, the composition weight proportioning of complex prebiotics is: soyabean oligosaccharides: FOS: xylo-oligosaccharide: gossypose=8: 5: 2: 5.
The composition weight proportioning of compound probiotic is: bifidobacterium infantis: bifidobacterium longum: lactobacillus acidophilus=3: 2: 2.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 4: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is as follows:
Milk (material of giving money as a gift) 50g
Desalted whey powder 25g
Granulated sugar 5g
Peanut oil 4g
Complex prebiotics 10g
Compound probiotic 1.0 * 10
6Cfu
B B-complex 0.023g
Composite mineral matter 0.5g
Nucleotides 0.05g
Taurine 0.04g
DHA 0.15g
ARA 0.1g
Choline 0.1g
Lecithin 0.2g.
Beta carotene 0.14g.
Wherein, the composition weight proportioning of complex prebiotics is: soyabean oligosaccharides: FOS: xylo-oligosaccharide: gossypose=10: 5: 2: 5.
The composition weight proportioning of compound probiotic is: bifidobacterium infantis: bifidobacterium adolescentis: lactobacillus acidophilus=3: 2: 2.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 5: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is as follows:
Goat milk (material of giving money as a gift) 40g
Desalted whey powder 15g
Granulated sugar 3g
Peanut oil 4g
Complex prebiotics 0.4g
Compound probiotic 1.0 * 10
5Cfu
B B-complex 0.023g
Composite mineral matter 0.5g
Nucleotides 0.05g
Taurine 0.04g
DHA 0.15g
ARA 0.1g
Choline 0.1g
Lecithin 0.2g
Beta carotene 0.14g.
Wherein, the composition weight proportioning of complex prebiotics is: oligoisomaltose: FOS: xylo-oligosaccharide=5: 10: 5.
The composition weight proportioning of compound probiotic is: bifidobacterium longum: bifidobacterium breve: lactobacillus bulgaricus=2: 4: 3.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 6: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is as follows:
Milk (material of giving money as a gift) 68g
Desalted whey powder 37g
Granulated sugar 6g
Peanut oil 8g
Complex prebiotics 20g
Compound probiotic 1.0 * 10
11Cfu
B B-complex 0.023g
Composite mineral matter 0.5g
Nucleotides 0.05g
Taurine 0.04g
DHA 0.15g
ARA 0.1g
Choline 0.1g
Lecithin 0.2g
Beta carotene 0.14g.
Wherein, the composition weight proportioning of complex prebiotics is: chitosan oligomer: soyabean oligosaccharides: inulin: milk ketose=2: 4: 2: 10.
The composition weight proportioning of compound probiotic is: baby's fork bacillus: bifidobacterium breve: Lactobacillus casei=1: 5: 2.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 7: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is as follows:
Milk (material of giving money as a gift) 54g
Desalted whey powder 26g
Granulated sugar 4g
Peanut oil 6g
Complex prebiotics 2g
Compound probiotic 1.0 * 10
8Cfu
B B-complex 0.023g
Composite mineral matter 0.5g
Nucleotides 0.05g
Taurine 0.04g
DHA 0.15g
ARA 0.1g
Choline 0.1g
Lecithin 0.2g
Beta carotene 0.14g.
Wherein, the composition weight proportioning of complex prebiotics is: oligoisomaltose: xylo-oligosaccharide: inulin: chitosan oligomer=2: 2: 8: 10.
The composition weight proportioning of compound probiotic is: baby's fork bacillus: bifidobacterium longum: lactobacillus acidophilus=5: 1: 3.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Embodiment 8: prepare a kind of milk powder that adds symphysis unit, wherein, the prescription of milk powder is as follows:
Milk (material of giving money as a gift) 60g
Desalted whey powder 20g
Granulated sugar 5g
Peanut oil 5g
Complex prebiotics 10g
Compound probiotic 1.0 * 10
14Cfu
B B-complex 0.023g
Composite mineral matter 0.5g
Nucleotides 0.05g
Taurine 0.04g
DHA 0.15g
ARA 0.1g
Choline 0.1g
Lecithin 0.2g
Beta carotene 0.14g.
Wherein, the composition weight proportioning of complex prebiotics is: FOS: soyabean oligosaccharides: xylo-oligosaccharide: milk ketose=5: 2: 8: 10.
The composition weight proportioning of compound probiotic is: bifidobacterium adolescentis: bifidobacterium longum: lactobacillus thermophilus=1: 8: 4.
The prescription of B B-complex is with embodiment 1.
The prescription of composite mineral matter is with embodiment 1.
Preparation technology is with embodiment 1.
Test example 1: following animal experiment has been described the comparison that complex prebiotics among the present invention improves the result to improvement and the single prebiotics of rat gut flora.
1 materials and methods:
1.1 animal used as test: 64 of SPF level SD male rats (Jinan Ge Run zoopery scientific and technological development Co., Ltd provides), body weight 100 ± 10g is divided into 8 groups at random, 8 every group.
1.2 grouping:
Group 1: control group: the basal feed of feeding.
Group 2: single prebiotics: basal feed adds 4% oligoisomaltose.
Group 3: single prebiotics: basal feed adds 4% galactooligosaccharide.
Group 4: single prebiotics: basal feed adds 4% soyabean oligosaccharides.
Group 5: complex prebiotics: (weight proportion of each component of prebiotics is the complex prebiotics of basal feed interpolation 4%: xylo-oligosaccharide 2+ galactooligosaccharide 10+ inulin 15).
Group 6: complex prebiotics: (weight proportion of each component of prebiotics is the complex prebiotics of basal feed interpolation 4%: oligoisomaltose 15+ xylo-oligosaccharide 2+ gossypose 5).
Group 7: complex prebiotics: (weight proportion of each component of prebiotics is the complex prebiotics of basal feed interpolation 4%: soyabean oligosaccharides 10+ FOS 5+ xylo-oligosaccharide 2+ inulin 15).
Group 8: complex prebiotics: (weight proportion of each component of prebiotics is the complex prebiotics of basal feed interpolation 4%: oligoisomaltose 15+ gossypose 5+ milk ketose 10+ inulin 15).
Each group is raised free diet separately.The feed of feeding all is SPF level feeds, drinks water to be pure water.
1.3 sample collection: feeding time respectively was 3 weeks, forced method get the rat fresh excreta respectively at the 0th day, 3 days, 7 days, 10 days, 14 days, 21 days after the experiment beginning, be loaded on and weigh and be equipped with in the sterile test tube of physiological saline and bead, test tube behind the dress sample is weighed once more, tries to achieve sample weight.Build the pipe post-games and be placed on the liquid quick oscillation device and mix,, become series with physiological saline with 10 times ratio doubling dilution then to make even suspension.
1.4 medium preparation: adopt the aerobic culture medium EMB of selectivity, EC, selectivity anaerobic culture medium KV, TPY and MRS are according to the requirement preparation of " Clinical Anaerobic Bacteria inspection manual ".
1.5 inoculated and cultured: the sample series dilution 0.01ml that gets above-mentioned processing is inoculated on the culture medium, places anaerobism and aerobic environment to cultivate respectively.37 ℃ of aerobic cultivations, 24h observed result, observed result behind the anaerobism 48h.The record clump count is also made identification of strains.
1.6 calculate and data statistics: the result represents with logarithm value, computing formula: the viable bacteria colony in every gram ight soil is counted logarithm value=log{[sample weight (g)+dilution liquid measure (ml)]/sample weight * dilution factor * bacterium colony number * 100}.All data are all analyzed with the SPSS statistical software.
2 results:
Adopt the aerobic culture medium EMB of selectivity, EC respectively, selectivity anaerobic culture medium KV, TPY and MRS detect and respectively organize flora in the rat stool sample 5: enterobacteria, enterococcus, bacteroid, Bifidobacterium and lactobacillus, testing result sees Table 1-5.
Table 1 is respectively organized rat stool sample enterobacteria group testing result [x ± s, x=log (CFU/g), n=8]
Annotate: a1, b1, c1, d1, e1 represent to have compared significant difference between two groups, P<0.05; A2, b2, c2 represent to have compared between two groups utmost point significant difference, P<0.01.
Table 2 is respectively organized rat stool sample enterococcus group testing result [x ± s, x=log (CFU/g), n=8]
Annotate: a1, b1, c1, d1, e1 represent to have compared significant difference between two groups, P<0.05; A2, b2, c2 represent to have compared between two groups utmost point significant difference, P<0.01.
Table 3 is respectively organized rat stool sample bacteroid group testing result [x ± s, x=log (CFU/g), n=8]
Annotate: a1, b1 represent to have compared significant difference between two groups, P<0.05.
Table 4 is respectively organized rat stool sample Bifidobacteria population testing result [x ± s, x=log (CFU/g), n=8]
Annotate: a1, b1, c1, d1, e1, f1, g1, h1 represent to have compared significant difference between two groups, P<0.05; A2, b2, c2 represent to have compared between two groups utmost point significant difference, P<0.01.
Table 5 is respectively organized rat stool sample lactobacillus group testing result [x ± s, x=log (CFU/g), n=8]
Annotate: a1, b1 represent to have compared significant difference between two groups, P<0.05; A2 represents to have compared between two groups utmost point significant difference, P<0.01.
Table 1, table 2 data all show, single prebiotics makes enterobacteria, enterococcus that the trend of minimizing be arranged, and that complex prebiotics reduces is more; Though table 3 shows the bacteroid of adding complex prebiotics the trend of minimizing is arranged, variation tendency is not obvious.Table 4, table 5 data show that compare with single prebiotics, complex prebiotics can make beneficial bacterium that good growth trend is arranged, but relative Bifidobacterium, the increase trend of lactobacillus is not clearly.
By above-mentioned test as can be seen, different prebioticses has very big difference to the influence of rat gut flora, and the complex prebiotics of various combination never is simple overlaying relation to the influence of rat gut flora, therefore, to add the complex prebiotics in the milk powder to be the complex prebiotics combination with optimum efficiency that obtains by test in the present invention.
Test example 2: following animal experiment described symphysis unit among the present invention to administration of antibiotics after rat recover the beneficial effect of beneficial bacterium advantage in the gut flora.
1 materials and methods:
1.1 animal used as test: 24 of SPF level SD male rats (Jinan Ge Run zoopery scientific and technological development Co., Ltd provides), body weight 100 ± 10g is divided into 3 groups at random, 8 every group.
1.2 grouping:
Group 1: control group: the basal feed of feeding
Group 2: experiment contrast group: the basal feed of feeding, 0 day administration of antibiotics (Lincomycin Hydrochloride, 200mg/Kg/ day, per os was irritated stomach) once
Group 3: experimental group: the basal feed of feeding, and administration of antibiotics, after 3 days, feeding, (weight proportion of each component of symphysis unit is the feed that adds 4% symphysis unit: xylo-oligosaccharide 2+ galactooligosaccharide 10+ soyabean oligosaccharides 10+ gossypose 5+ bifidobacterium infantis 10
3Cfu/g+ bifidobacterium adolescentis 10
2Cfu/g+ lactobacillus acidophilus 10
2Cfu/g)
1.3 sample collection: feeding time was 3 weeks, forced method get the rat fresh excreta respectively at the 0th, 3,7,14,21 day after the experiment beginning, be loaded on and weigh and be equipped with in the sterile test tube of physiological saline and bead, the test tube behind the dress sample is weighed once more, tries to achieve sample weight.Build the pipe post-games and be placed on the liquid quick oscillation device and mix,, become series with physiological saline with 10 times ratio doubling dilution then to make even suspension.
1.4 medium preparation: adopt the aerobic culture medium EMB of selectivity, EC, selectivity anaerobic culture medium KV, TPY and MRS are according to the requirement preparation of " Clinical Anaerobic Bacteria inspection manual ".
1.5 inoculated and cultured: the sample series dilution 0.01ml that gets above-mentioned processing is inoculated on the culture medium, places anaerobism and aerobic environment to cultivate respectively.37 ℃ of aerobic cultivations, 24h observed result, observed result behind the anaerobism 48h.The record clump count is also made identification of strains.
1.6 calculate and analyze: the result represents with various bacterium proportions
Computing formula:
Viable bacteria colony number in every gram ight soil=[sample weight (g)+dilution liquid measure (ml)]/sample weight * dilution factor * bacterium colony number
2 results:
Adopt the aerobic culture medium EMB of selectivity, EC respectively, selectivity anaerobic culture medium KV, TPY and MRS detect and respectively organize flora in the rat stool sample 5: enterobacteria, enterococcus, bacteroid, Bifidobacterium and lactobacillus, and calculate, testing result sees Table 6.
Table 6 symphysis unit is to the experimental result that influences of big gut flora
From experimental data as can be seen, the recovery of the rat gut flora of symphysis unit after for administration of antibiotics and beneficial bacterium advantage establishes good benefit.
Test example 3: following test has been described the baby formula milk powder of interpolation symphysis of the present invention unit to diarrhoea infant's clinical effectiveness takes place.
1 data and method
1.1 Object Selection: select in August, 2008-2008 year September, certain hospital-based outpatient pediatic clinic infantile diarrhea 120 example, 7 months-3 years old age.Clinical manifestation meets following characteristics: (1) sick first have slight upper respiratory tract infection symptoms (runny nose, idol are coughed), heating, vomiting; (2) stool is rare water sample or egg soup sample, do not have and smell as of rotten fish, and no purulence blood, number of times 24h surpasses more than 3 times; (3) the light or moderate dehydration of companion.
1.2 method: whole cases are divided into treatment at random organize 60 examples, control group 60 examples, the state of an illness, the course of disease are learned the processing no significant difference by statistics between two groups.
The treatment group: the baby milk powder among the edible embodiment 1, every day consumption 100g
Control group: dioctahedral smectite treatment, dosage: age<1 year old is 1g, and 1~2 years old is 1~1.5g, and 2~3 years old is 2g, serve on 3d every day three times, normal diet (preventing to bring in the diet any probio or prebiotics composition)
1.3 the result judges: by the curative effect determinate standard that the sick meeting of national baby diarrhea is formulated, produce effects: refer to medication 24h-48h, times of defecation is less than 2 times/d or recovers normal number of times, and the stool proterties recovers normal, clinical symptom disappearance; Effectively: refer to medication 24h-72h, times of defecation is less than 2 times/d, and proterties takes a turn for the better, and moisture reduces, and clinical symptoms disappears substantially; Invalid: refer to 72h after the medication, stool does not have more than 2 times/d or proterties improves even the person that increases the weight of to change the medicine.Take effect slowly because of symphysis unit, its treatment time extended to for 1 week.
2 results: according to criterion, two groups result of treatment sees Table 7
Table 7 baby formula milk powder is to the result of treatment of infantile diarrhea
See that from result of the test the baby formula milk powder among the present invention has the curative effect the same with dioctahedral smectite to infantile diarrhea to find out.Baby milk powder is adjusted the balance of infant's gut flora by adding symphysis unit, suppresses the breeding of pathogenic bacteria, thereby reaches the therapeutic action to infantile diarrhea.Because this product is to come diarrhoea is prevented by diet, can not bring the side effect of chemicals such as dioctahedral smectite, has bigger advantage.
Claims (8)
1. a baby milk powder that adds symphysis unit is characterized in that, comprises following component: the fresh milk material of giving money as a gift, 40~68 grams; Desalted whey powder, 15~37 grams; Granulated sugar, 3~6 grams; Vegetable oil, 4~8 grams; Complex prebiotics, 0.4~20 gram; Compound probiotic 1.0 * 10
5~1.0 * 10
14Cfu;
Described complex prebiotics is one of following combination:
1. 3 parts+gossypose of 10 parts+xylo-oligosaccharide of oligoisomaltose is 5 parts;
2. 10 parts+inulin of 2 parts+galactooligosaccharide of xylo-oligosaccharide is 15 parts;
3. 2 parts+gossypose of 15 parts+xylo-oligosaccharide of oligoisomaltose is 5 parts;
4. 2 parts+inulin of 5 parts+xylo-oligosaccharide of 10 parts+FOS of soyabean oligosaccharides is 15 parts;
5. 10 parts+inulin of 5 parts+milk ketose of 15 parts+gossypose of oligoisomaltose is 15 parts;
Described umber is a weight portion;
Described compound probiotic is a kind of or several arbitrary proportions combination in bifidobacterium strains, lactobacillus strains, streptococcus bacterial strain, lactococcus bacterial strain, the Bacillus strain.
2. a kind of baby milk powder that adds symphysis unit according to claim 1 is characterized in that, also comprises following component: B B-complex, 0.023 gram; Composite mineral matter, 0.5 gram; Nucleotides, 0.05 gram; Taurine, 0.04 gram; DHA, 0.15 gram; Arachidonic acid, 0.1 gram; Lecithin, 0.2 gram; Beta carotene, 0.14 gram.
3. a kind of baby milk powder that adds symphysis unit according to claim 1 is characterized in that, the content of described complex prebiotics is 2~10 grams.
4. a kind of baby milk powder that adds symphysis unit according to claim 1 is characterized in that: described compound probiotic is the combination of bifidobacterium strains and lactobacillus strains.
5. a kind of baby milk powder that adds symphysis unit according to claim 4 is characterized in that: described bifidobacterium strains is bifidobacterium infantis or bifidobacterium adolescentis or bifidobacterium longum or bifidobacterium breve; Described lactobacillus strains is lactobacillus bulgaricus or lactobacillus acidophilus or lactobacillus thermophilus or Lactobacillus casei.
6. a kind of baby milk powder that adds symphysis unit according to claim 2 is characterized in that described B B-complex is composed of the following components: vitamin A, 4 grams; Vitamin D, 2 grams; Vitamin E, 0.1 gram; Vitamin K
1, 0.035 gram; Cobastab
1, 0.6 gram; Cobastab
2, 0.8 gram; Cobastab
6, 0.35 gram; Cobastab
12, 0.015 gram; Vitamin C, 2 grams; Niacinamide usp, 8 grams; Folic acid, 0.08 gram; Calcium pantothenate, 5 grams; Biotin, 0.02 gram.
7. a kind of baby milk powder that adds symphysis unit according to claim 2 is characterized in that described composite mineral matter is composed of the following components: ferrous lactate, 50 grams; Zinc lactate, 10 grams; Copper sulphate, 2 grams; Potassiumiodate, 0.13 gram; Calcium carbonate, 437.7 grams; Manganese sulfate, 0.17 gram.
8. the described a kind of preparation method who adds the baby milk powder of symphysis unit of claim 2, it is characterized in that, step is: mix other components except that compound probiotic, B B-complex, get mixed solution, use high pressure homogenizer under 10~20MPa, to carry out homogeneous then, carry out spray-drying or freeze drying then, get powder product, add compound probiotic, B B-complex then, mixing, vacuum packaging.
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