CN101422422A - Sheep placental extract paste and manufacture method thereof - Google Patents
Sheep placental extract paste and manufacture method thereof Download PDFInfo
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- CN101422422A CN101422422A CNA2008102201550A CN200810220155A CN101422422A CN 101422422 A CN101422422 A CN 101422422A CN A2008102201550 A CNA2008102201550 A CN A2008102201550A CN 200810220155 A CN200810220155 A CN 200810220155A CN 101422422 A CN101422422 A CN 101422422A
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Abstract
The invention relates to a placenta paste and a manufacture method thereof. The placenta paste is used for a skin care product and discloses a substrate which comprises 2 to 6 percent of the compound additive of panaxoside concrete exacted by supercritical carbon dioxide and a sheep placenta extract by hydrolyzing as well as water accounting for the rest percent. The invention can effectively and safely extract the effective components in plants, can effectively change the physical property of an animal extract, is beneficial to the absorption of a human skin and has remarkable crease-resistant effects to the human skin.
Description
Technical field
The present invention relates to the skin care item in people's daily life, specifically a kind of crease-resistant skin care item.
Background technology
Skin care item are of a great variety, but its function can reduce skin moistening, whitens, and are wrinkle resistant, and these function skin care item all are to add a certain amount of functional additive to form in skin care item substrate.With regard to its functional additive plant extract additive, animal extracts additive are arranged, mineral extract additive.As many now the methods of plant extract additive with water extraction, organic solvent extraction.This two quasi-traditions extracting method all has the drawback part, the one, Yin Wendu is too high and destroyed the effective ingredient of plant, the 2nd, organic solvent residual, destroyed product quality and result of use to a certain extent, the method extraction effect that simultaneously this tradition is extracted is poor, in extract, effective ingredients in plant content is low, in the Radix Ginseng extractive solution, content of ginsenoside is lower than 3%, causes the weak effect of skin care item.The situation of animal extracts additive is also very similar, causes content low with traditional extracting method, adds in the skin care item and is used for the human body weak effect.A lot of such as the deutero-product of Placenta caprae seu ovis, Chinese patent application number 02160340.5 has the Foetus Caprae seu Ovis lyophilized powder in the compositions in a kind of Foetus Caprae seu Ovis peptide capsule, capsule.Its method for making is that pretreated Placenta caprae seu ovis homogenate is carried out micronized pulverization with colloid mill, broken liquid precipitates through 4000 to 6000 rev/mins centrifugal going, supernatant is under-25 ℃～30 ℃ cryogenic conditions, pre-freeze 6～8 hours, in vacuum freeze drier, carry out drying then, promptly get the Foetus Caprae seu Ovis lyophilized powder.This Foetus Caprae seu Ovis lyophilized powder is directly joined in the skin care item, and the prolonged and repeated use of people is after on the skin, and what new effect is not seen has.
Summary of the invention
One of purpose of the present invention provides a kind of sheep placental extract paste, and it has pair human body skin that tangible anti-wrinkle effect is arranged.
Another purpose of the present invention provides the preparation method of crease-resistant additive, and the effective ingredient in the extraction plant that can be effective, safe can change the physical attribute of animal extracts effectively, helps human body skin and absorbs.
A kind of sheep placental extract paste technical solution of the present invention is, percent calculates by weight, comprises the ginsenoside's extractum through supercritical carbon dioxide extraction, and is that additive is 2%～6% through the compositions of the Placenta caprae seu ovis extract of hydrolysis, and surplus is a skin care item substrate.
The above ginsenoside's extractum through supercritical carbon dioxide extraction is 2%～5%.
The compositions of the Placenta caprae seu ovis extract through hydrolysis of the present invention is 2%～6%.
More than Placenta caprae seu ovis extract of the present invention be frozen dry powder of sheep placenta.
Skin care item substrate of the present invention comprises composes fat agent 5%～15%, emulsifying agent 0.8%～4.5%, wetting agent 5%～8%, antiseptic 0.05～0.20%, odorant, chelating agent, antioxidant, deionized water surplus.
The manufacture method of the additive of a kind of sheep placental extract paste of the present invention:
The manufacture method of ginsenoside's extractum of A, supercritical carbon dioxide extraction,
The powder of exsiccant Radix Ginseng through pulverizing is dropped in the extraction kettle, and the extraction time is 100min; Extraction temperature is 40 ℃; Extracting pressure is 15Mpa; CO
2Flow velocity is 12kg/kgh; Separating pressure is: detached dowel is 6MPa, and parsing I is that 10Mpa, temperature are 44 ℃, and parsing II is that 5.6MPa, temperature are 25 ℃; The entrainer that adopts is an ethanol, and extract promptly is ginsenoside's extractum.Its standard is as follows:
| Project | Index |
| Outward appearance and organoleptic attribute | This product is yellow to the yellowish-brown paste, has the Radix Ginseng characteristic odor. |
| Ginsenoside's total content (μ g/ml) | 〉=30 |
| Moisture, % | ≤ 10.0 |
| Acid number, mgKOH/g | ≤ 20 |
| Content of beary metal | Plumbous (Pb) content (mg/kg):＜1; Arsenic (As) content (mg/kg):＜1; Hydrargyrum (Hg) content (mg/kg):＜0.1 |
| Micro biological Tests | Total plate count (cfu/g):＜1000; Mycete (cfu/g):＜100 |
B, through the manufacture process of the Placenta caprae seu ovis extract of hydrolysis: the composition and the content difference of the Placenta Hominis active substance in different gestation stages are very big, and the effect of Placenta caprae seu ovis element is the synergism of various active material, and its effect depends on the content of active substance in the finished product.Studies show that the contained bioactive ingredients of Placenta Hominis in 14 weeks of trimester of pregnancy is the highest.So adopting the Healthy Sheep Placenta Hominis and the embryo thereof in quarantine qualified hundred day age of trimester of pregnancy less than is raw material, adopts the cryogenic vacuum Freeze Drying Technique to extract, and concentrates and purifies, by scientific process forms, and bioactive substance in the lyophilized powder and nutrition are 25 times of bright Placenta Hominis.The present invention is again with frozen dry powder of sheep placenta
Soak
Enzymolysis
Decolouring
Sterilization
Filter
Finished product.
(1), soak: the ratio in frozen dry powder of sheep placenta: Purified Water=8:100 is soaked,
(2), enzymolysis: at 50 ℃, add protease amount 2%, pH8.0, the hydrolysis result of enzymolysis 8h is best, chromatography finds, it is more comprehensive that hydrolysis temperature is that 50 ℃ extracting solution contains aminoacid, and the aminoacid automatic analyzer is measured and shown, amino acid whose content is greater than hot water extraction method's content in the Placenta caprae seu ovis extracting solution of protease hydrolyzed method
(3), boil: room temperature, 400MPa, pressurization 20min alternation 2 times (pressurization 5min is 2min at interval) helps reducing amino acid whose loss,
(4), decolouring: granular active carbon is obvious to the decolorizing effect of Placenta caprae seu ovis extracting solution; at ratio of height to diameter is 4; during flow velocity 1.8mL/min; help the decolouring of extracting solution, treating capacity is 42mL/g, and decolouring back extracting solution transmittance is more than 75%; simultaneously can be by mass concentration 7%NaOH+2%HCl desorption and regeneration at an easy rate; decolouring and desorption effect are best in the time of 80 ℃, can satisfy the requirement of the transparent Placenta caprae seu ovis extracting solution of preparation
(5), sterilization: room temperature, 400MPa, pressurization 20min alternation 4 times (pressurization 5min is 2min at interval) helps reducing amino acid whose loss,
(6), filter: use vacuum pumping rate machine, the aperture is that 0.2 μ m filter stick filters.
Specific targets are as follows:
| Project | Index |
| Outward appearance | Amber transparency liquid |
| Amino acid nitrogen content | ≥0.4％ |
| PH value | 5.5～6.5 |
| The working substance total solids content | ≥4.0％ |
| Ash | ≤0.05％ |
| Mean molecule quantity | 200～2000 |
C, in proportion with ginsenoside's extractum with through the first before use mix homogeneously of the Placenta caprae seu ovis extract of hydrolysis, by the time 55 ℃ the time, scattered mixed material is dropped in the mastic.
The various materials of the present invention:
1) wetting agent: the water absorbing properties of glycerol is 39% of a weight, has stronger absorbability.Use simultaneously with sorbitol, the potentiation synergism is arranged.Propylene glycol can be used as wetting agent with glycerol or sorbitol in cosmetics as the succedaneum of glycerol, and total amount generally maintains 5-15% between the two.
2) compose the fat agent:
The agent of liquid tax fat: sad/certain herbaceous plants with big flowers acid glycerol three esters and isopropyl palmitate can be used for O/W or w/o type product in cosmetics, two kinds of light-weight oils and fatss, and the skin sense is comparatively salubrious, and use suitable summer, but price is higher.And lanoline, Dormant oils greasy feeling are planted, and are fit to use winter, and price is comparatively cheap simultaneously.In general, both cost performances of arranging in pairs or groups are comparatively reasonable.Be applicable to southern product, consumption generally maintains 5-10% between the two, and at northern product sold, consumption is generally at 10-20%.
Solid is composed the fat agent: the contrast liquid fat, and both have obvious thickening capabilities spermol, spermol cetylate, but that the shop is coated with ability is poor than liquid, so consumption is generally 1-3%.
3) emulsifying agent:
Sodium lauryl sulfate: anion emulsifier, emulsifying capacity is stronger, but bigger to the zest of skin; Comparatively few using.
The PEG-60 Span60, polysorbate-60: nonionic emulsifier, when pairing was used, the particle diameter of mastic was more tiny and even, and stablizing effect is better; Consumption is according to the decision of the HLB value of system, consumption and be 3-4% in this prescription
Ceteareth-6, ceteareth-25: nonionic emulsifier, when pairing was used, the particle diameter of mastic was more tiny and even, and stablizing effect is better; Consumption also can use in this prescription according to the decision of the HLB value of system, consumption and be 3.5-4.5%
4) additive:
Ginsenoside: because of it contains various nutrients, can increase the vigor of cell, and the effect that enhances metabolism with the peripheral vessel circulation is arranged.Be used for skin care item, can make skin smooth, soft flexible, slow down aging also can suppress melanin and produce.Consumption is generally 2-5%
Hydrolysis intacellin (sheep): owing to contain multiple physiologically active ingredient in the Placenta Hominis, evidence is lived and metabolic effect has facilitation to the tax of Skin Cell.Wherein material such as mucopolysaccharide can suppress scattering and disappearing of horny layer moisture, prevents skin aging and wrinkle resistant effect thereby reach.Consumption is generally 2-6%
Tocopherol (vitamin E): skin is worked the effect of moistening, and content is to reduce dehydration 19% after 5% cream frost is embrocated 30min, reduces dehydration 24% after twice, four day every day, and it is to tell on by its osmosis.Consumption is generally 2-5%
5) antiseptic:
The oxybenzene ester is that first is applied to the antiseptic in the medicine, and they have antibacterial activity widely, the addition that uses methyl ester more be 0.2% and the amount of adding propylparaben be 0.1% mixture.
Two imidazolidinyl urea: be the donor of formaldehyde, it is to have antiyeast, mycete and bacterial action, and its antibacterial activity is better than imidazolidinyl urea.Highly stable in pH3～9 scopes, and compatible with the basic ingredient in the cosmetics.Consumption is generally 0.2-0.5%
Methylisothiazolinone: it is 0.05%～0.1% o'clock in concentration, has the activity of very strong antiyeast, mycete and antibacterial.
6) chelating agent: as ethylenediaminetetraacetic acid, be used for the metal ion of complex system, consumption is generally 0.05-0.2%
7) antioxidant: as Butylated hydroxyanisole, be the good antioxidant additive of various oil and fat products, consumption is generally 0.1-1%.
In a kind of sheep placental extract paste of the present invention because of adding additive in ginsenoside's extractum of supercritical carbon dioxide extraction, content of ginsenoside is more than 7030%, changed the physical attribute of Placenta caprae seu ovis extract through the hydrolysis Placenta caprae seu ovis extract, and ginsenoside's extractum of the present invention with through the mutual synergism of the Placenta caprae seu ovis extract of hydrolysis, to the crease-resistant positive effect that played of human body skin.
Description of drawings
Fig. 1 is that two groups of experimenters use the aqueous variation diagram of product 8 week back skin;
Fig. 2 is the variation diagram that two groups of experimenters use 8 week of product back skin elasticity index;
Fig. 3 is the variation diagram that two groups of experimenters use 8 week of product back skin coarseness parameter.
The specific embodiment
Manufacture method of the present invention
The manufacture method of ginsenoside's extractum of A, supercritical carbon dioxide extraction,
The powder of exsiccant Radix Ginseng through pulverizing is dropped in the extraction kettle, and the extraction time is 100min; Extraction temperature is 40 ℃; Extracting pressure is 15Mpa; CO
2Flow velocity is 12kg/kgh; Separating pressure is: detached dowel is 6MPa, and parsing I is that 10Mpa, temperature are 44 ℃, and parsing II is that 5.6MPa, temperature are 25 ℃; The entrainer that adopts is an ethanol, and extract promptly is ginsenoside's extractum.
B, through the manufacture method of the Placenta caprae seu ovis extract of hydrolysis, (1), soak: the ratio in frozen dry powder of sheep placenta: Purified Water=8:100 is soaked,
(2), enzymolysis: at 50 ℃, add protease amount 2%, pH8.0, the hydrolysis result of enzymolysis 8h is best, chromatography finds, it is more comprehensive that hydrolysis temperature is that 50 ℃ extracting solution contains aminoacid, and the aminoacid automatic analyzer is measured and shown, amino acid whose content is greater than hot water extraction method's content in the Placenta caprae seu ovis extracting solution of protease hydrolyzed method
(3), boil: room temperature, 400MPa, pressurization 20min alternation 2 times (pressurization 5min is 2min at interval) helps reducing amino acid whose loss,
(4), decolouring: granular active carbon is obvious to the decolorizing effect of Placenta caprae seu ovis extracting solution; at ratio of height to diameter is 4; during flow velocity 1.8mL/min; help the decolouring of extracting solution, treating capacity is 42mL/g, and decolouring back extracting solution transmittance is more than 75%; simultaneously can be by mass concentration 7%NaOH+2%HCl desorption and regeneration at an easy rate; decolouring and desorption effect are best in the time of 80 ℃, can satisfy the requirement of the transparent Placenta caprae seu ovis extracting solution of preparation
(5), sterilization: room temperature, 400MPa, pressurization 20min alternation 4 times (pressurization 5min is 2min at interval) helps reducing amino acid whose loss,
(6), filter: use vacuum pumping rate machine, the aperture is that 0.2 μ m filter stick filters.
C, in proportion with ginsenoside's extractum with through the first before use mix homogeneously of the Placenta caprae seu ovis extract of hydrolysis, by the time 55 ℃ the time, scattered mixed material is dropped in the mastic.
| Embodiment one | Embodiment two | Embodiment three | |
| The A group | |||
| Spermol | 2 | 2.5 | 3 |
| Isopropyl palmitate | 4 | 5 | 6 |
| Glyceryl monostearate | 1 | 1 | 1 |
| Sad/certain herbaceous plants with big flowers acid glycerol three esters | 5 | 6 | 7 |
| Ceteareth-6 | 2 | 2 | 2 |
| Butylated hydroxyanisole | 0.1 | 0.1 | 0.1 |
| Methyl parahydroxybenzoate | 0.1 | 0.1 | 0.1 |
| Propyl p-hydroxybenzoate | 0.1 | 0.1 | 0.1 |
| The B group | |||
| Sorbitol | 5 | 6 | 7 |
| Glycerol | 9 | 8 | 7 |
| Ethylenediaminetetraacetic acid | 0.05 | 0.05 | 0.05 |
| Ceteareth-25 | 2 | 2.2 | 2.5 |
| Deionized water | Surplus | Surplus | Surplus |
| The C group | |||
| The ginsenoside | 2 | 3 | 5 |
| The hydrolysis intacellin | 2 | 4 | 6 |
| The D group | |||
| Two imidazolidinyl urea | 0.3 | 0.3 | 0.3 |
| Tocopherol (vitamin E) | 2 | 2.2 | 2.5 |
| The E group | |||
| Essence | 0.15 | 0.15 | 0.15 |
Test method of the present invention and design
Test method and number adopt open, at random, the test of parallel control clinical efficacy, test number 40 people use 3 every day, observe 2 months, be divided into " skin lotion 1 (B1) group of no additive of the present invention " and " sheep placental extract paste 2 of the present invention (B2) group " at random, every group of each 20 people.
2. the experimenter selects
2.1.1 the age 25 was to 55 years old healthy women;
2.1.2 skin photo bio type is II to an IV type skin;
2.1.3 the Guangzhou life is lived more than 1 year;
2.1.4 the fine compatibility test person of energy can keep regularity in one's life and can avoid the daylight ultraviolet radiation during studying;
Problems such as 2.1.5 facial area skin has tiny wrinkle, elasticity is relatively poor and gloomy;
2.2 exclusion standard is all to have one of following condition person and must get rid of and enter this research:
2.2.1 face is associated with bacterial infection, virus or fungal infection person;
2.2.2 the person that has the allergic constitution;
2.2.3 intentionally, liver, renal function injury and immunologic hypofunction person;
2.2.4 spiritedness disease and endocrinopathy person or gestation and oral contraceptive person;
2.2.5 face has glucocorticoid dermatitis person;
2.2.6 the facial patient that eczema or seborrheic dermatitis, acne are arranged;
2.2.7 participated in clinical drug trial or other experimenter or oral in 30 days to the influential hormone medicine of result;
2.2.8 there is the oral and external may be in 2 weeks to the influential cosmetics of result of the test;
2.2.9 can not compatibility test person.
2.3 withdraw from the standard volunteer as gestation, adverse events during studying, occurring, or violate research approach and researcher and think that the experimenter will withdraw from this clinical research when being not suitable for continuing finishing other situations of this research, writes exactly simultaneously and withdraws from reason.
3. be subjected to trial product, product using method and pot life
3.1 be subjected to trial product be subjected to trial product be " seven days fragrant tire plain beauty cream 1 " and " the plain beauty cream 2 of fragrant tire on the seven " (Main Ingredients and Appearance be respectively blank and added " ginsenoside 2%, hydrolysis intacellin 2%)
3..2 the apparatus measures measurement index has moisture content of skin, skin elasticity, the analysis of skin microgroove.
3.3.1 doctor's assessment uses front and back experimenter's skin index of correlation to change the degree of judging that skin improves by observing product.Evaluation index comprises texture, skin moisturizing, skin elasticity, skin gloss, the bright property of skin, the tiny wrinkle of skin and skin smooth degree.Standards of grading: 0=does not have improvement or variation, and 1=has slight improvement, and the 2=moderate is improved, and 3=obviously improves, and 4=obviously improves or improves fully.
3.3.2 experimenter's self assessment adopts the form of questionnaire table to carry out, and assesses product voluntarily by the experimenter and uses front and back skin index of correlation to change the degree of judging that skin improves.Evaluation item comprises: the skin lightness, make skin look/feel more soft, make skin look/sensuously more smooth, make skin feel to moisture with moisten and can see that your skin changes when looking in the mirror.Standards of grading: selected by 0-3 minutes, 0=does not have improvement substantially, and 1=slightly improves, and the 2=moderate is improved, and 3=obviously improves or improves fully.
3.4 the apparatus measures measurement index has moisture content of skin, skin elasticity, the analysis of skin microgroove.
3.4.1 the skin moisture measuring instrument (Corneometer that the mensuration of moisture content of skin uses German COURAGE+KHAZAKA (CK) company to produce
CM825), measure experimenter's moisture content of skin; The moisture-keeping efficacy (, evaluation criterion: front and back difference 0～5 is for there being improvement, 5～10 have more obviously and improve, and be obvious improvement greater than 10) of this product is assessed in the variation of moisture content of skin numerical value before and after using by comparative product.
3.4.2 the skin elasticity measuring instrument that the mensuration of skin elasticity index uses German COURAGE+KHAZAKA (CK) company to produce is measured experimenter's skin elasticity index; The variation of skin elasticity index value is assessed this product to the influence of skin elasticity (, evaluation criterion: front and back difference 0～0.05 is for there being improvement, 0.05～0.1 has more obviously and improves, and be obvious improvement greater than 0.1) before and after using by comparative product.
3.4.3 the wrinkle of skin measuring instrument (SV600) that the mensuration of skin microgroove uses German COURAGE+KHAZAKA (CK) company to produce is measured experimenter's skin coarseness parameter; The variation of skin coarseness parameter values is assessed this product to the influence of skin microgroove (, evaluation criterion: front and back difference 0～5 is for there being improvement, 5～10 have more obviously and improve, and be obvious improvement greater than 10) before and after using by comparative product.
4.4 apparatus measures result
4.4.1 the variation of moisture content of skin: two groups of experimenters are using product 1 all backs moisture content of skin all than obviously increasing (P＜0.05) before using, reach higher level during 2 weeks, after this along with the product prolongation of service time, experimenter's moisture content of skin reduces to some extent, but still maintains higher level (seeing Fig. 1 for details).But from chart, using product to begin after 2 weeks, B2 group experimenter skin moisture-keeping effect is better than the B1 group." B1 " and " B2 " has 85% and 80% experimenter using 2 all rear face moisture content of skin to begin to increase respectively, after using for 8 weeks, have experimenter's skin of face water content of 75% and 84.2% that improvement is arranged respectively, wherein 45% and 57.9% experimenter improves apparent in view.
The change of skin elasticity: as shown in Figure 2, using product after 4 weeks, having clear improvement (P＜0.05) before B2 group experimenter skin elasticity index is used, and B1 group experimenter skin elasticity index use before nothing obviously improve (P〉0.05).After product uses 2 weeks, 4 weeks, 6 weeks and 8 weeks, comparison there was no significant difference between each time period skin elasticity index improvement degree of two groups of experimenters (P〉0.05), but from chart, using product to begin after 2 weeks, the degree that B2 group experimenter skin elasticity index is improved is better than the B1 group, statistics goes up there was no significant difference between two groups, may be not enough with observing time and to observe routine number relevant inadequately." B1 " and " B2 " has 65% and 52.6% tester using 2 all rear face skin elasticity indexs to begin to increase respectively, after using for 8 weeks, have experimenter's skin of face elasticity of 65% and 78.9% that improvement is arranged respectively, wherein 30% and 42.1% experimenter improves apparent in view.
The change of skin microgroove: using product after 8 weeks, B1 group and B2 group experimenter's skin coarseness parameter is all than (P＜0.05) (the seeing chart 3) that improve significantly before using.After product uses 2 weeks, 4 weeks, 6 weeks and 8 weeks, comparison there was no significant difference between the degree that each time period skin coarseness parameter reduces of two groups of experimenters (P〉0.05), but from chart, using product to begin after 2 weeks, the degree that B2 group experimenter skin coarseness parameter is improved is better than the B1 group, statistics goes up there was no significant difference between two groups, may be not enough with observing time and to observe routine number relevant inadequately." B1 " and " B " has 60% and 73.7% tester using 2 all rear face skin coarseness to begin to reduce respectively, after using for 8 weeks, have experimenter's skin of face roughness of 65% and 68.4% that improvement is arranged respectively, wherein 40% and 42.1% experimenter use 8 week the back improve apparent in view.
Claims (6)
1, a kind of sheep placental extract paste is characterized in that by weight percent calculates, and comprises the ginsenoside's extractum through supercritical carbon dioxide extraction, and is 2%～6% through the compositions additive of the Placenta caprae seu ovis extract of hydrolysis, and surplus is a skin care item substrate.
2, a kind of sheep placental extract paste according to claim 1 is characterized in that the ginsenoside's extractum through supercritical carbon dioxide extraction is 2%～5%.
3, a kind of sheep placental extract paste according to claim 1 is characterized in that the Placenta caprae seu ovis extract through hydrolysis is 2%～6%.
4, according to claim 1 or 3 described a kind of sheep placental extract pastes, it is characterized in that described Placenta caprae seu ovis extract is a frozen dry powder of sheep placenta.
5, a kind of sheep placental extract paste according to claim 1, it is characterized in that skin care item substrate comprises tax fat agent 5%～15%, emulsifying agent 0.8%～4.5%, wetting agent 5%～8%, antiseptic 0.05～0.20%, odorant, chelating agent, antioxidant, deionized water surplus.
6, a kind of manufacture method of additive of sheep placental extract paste is characterized in that:
The manufacture method of ginsenoside's extractum of A, supercritical carbon dioxide extraction,
The powder of exsiccant Radix Ginseng through pulverizing is dropped in the extraction kettle, and the extraction time is 100min; Extraction temperature is 40 ℃; Extracting pressure is 15Mpa; CO
2Flow velocity is 12kg/kgh; Separating pressure is: detached dowel is 6MPa, and parsing I is that 10Mpa, temperature are 44 ℃, and parsing II is that 5.6MPa, temperature are 25 ℃; The entrainer that adopts is an ethanol, and extract promptly is ginsenoside's extractum;
B, through the manufacture method of the Placenta caprae seu ovis extract of hydrolysis: with frozen dry powder of sheep placenta according to the following steps: immersion, enzymolysis, decolouring, sterilization, filtration, finished product;
(1), soak: the ratio in frozen dry powder of sheep placenta: Purified Water=8:100 is soaked,
(2), enzymolysis: at 50 ℃, add protease amount 2%, pH8.0, the hydrolysis result of enzymolysis 8h is best, chromatography finds, it is more comprehensive that hydrolysis temperature is that 50 ℃ extracting solution contains aminoacid, and the aminoacid automatic analyzer is measured and shown, amino acid whose content is greater than hot water extraction method's content in the Placenta caprae seu ovis extracting solution of protease hydrolyzed method
(3), boil: room temperature, 400MPa, pressurization 20min alternation 2 times (pressurization 5min is 2min at interval) helps reducing amino acid whose loss,
(4), decolouring: granular active carbon is obvious to the decolorizing effect of Placenta caprae seu ovis extracting solution; at ratio of height to diameter is 4; during flow velocity 1.8mL/min; help the decolouring of extracting solution, treating capacity is 42mL/g, and decolouring back extracting solution transmittance is more than 75%; simultaneously can be by mass concentration 7%NaOH+2%HCl desorption and regeneration at an easy rate; decolouring and desorption effect are best in the time of 80 ℃, can satisfy the requirement of the transparent Placenta caprae seu ovis extracting solution of preparation
(5), sterilization: room temperature, 400MPa, pressurization 20min alternation 4 times (pressurization 5min is 2min at interval) helps reducing amino acid whose loss,
(6), filter: use vacuum pumping rate machine, the aperture is that 0.2 μ m filter stick filters,
C, in proportion with ginsenoside's extractum with through the first before use mix homogeneously of the Placenta caprae seu ovis extract of hydrolysis, by the time 55 ℃ the time, scattered mixed material is dropped in the mastic.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108771638A (en) * | 2018-07-22 | 2018-11-09 | 张晓南 | A kind of biomass placenta freeze-dried powder preparation and preparation method thereof |
| CN109731025A (en) * | 2018-11-08 | 2019-05-10 | 袁海龙 | It is a kind of for treating compound skin cream, preparation method and the application of wrinkle of skin |
-
2008
- 2008-12-19 CN CNA2008102201550A patent/CN101422422A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108771638A (en) * | 2018-07-22 | 2018-11-09 | 张晓南 | A kind of biomass placenta freeze-dried powder preparation and preparation method thereof |
| CN109731025A (en) * | 2018-11-08 | 2019-05-10 | 袁海龙 | It is a kind of for treating compound skin cream, preparation method and the application of wrinkle of skin |
| CN109731025B (en) * | 2018-11-08 | 2021-10-08 | 中国人民解放军空军特色医学中心 | Compound skin cream for treating skin wrinkles, preparation method and application thereof |
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