CN101398353B - Gel dyeing apparatus and method - Google Patents
Gel dyeing apparatus and method Download PDFInfo
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- CN101398353B CN101398353B CN2008102024217A CN200810202421A CN101398353B CN 101398353 B CN101398353 B CN 101398353B CN 2008102024217 A CN2008102024217 A CN 2008102024217A CN 200810202421 A CN200810202421 A CN 200810202421A CN 101398353 B CN101398353 B CN 101398353B
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Abstract
The invention relates to a gel dyeing device and a method, wherein, the device comprises a gel dyeing container; the gel dyeing device consists of a hollow chassis (1) where hollow holes are uniformly distributed, a dismountable hollow frame (2) which is matched with the hollow chassis, and a mainbody device which is composed of the hollow chassis and the hollow frame; the hollow chassis is provided with a layer of corresponding replaceable hollow film (3); a degumming container and a sealable dyeing container as well as the mainbody device form a whole set of gel dyeing device in a matching way; during the whole process, hands are almost not directly contacted with the gel, thus ensuring no-damage of the gel and extremely facilitating the subsequent photographing, observing and glue-cutting operation; the application of the device greatly saves the time, provides extremely great convenience for the subsequent gel observation and glue-cutting operation and the like, provides guarantee for obtaining the gel images with good integration and high quality, reduces unnecessary pollutions during the operation process and ensures the safety of the operators more.
Description
Technical field
The invention belongs to biological technical field, particularly a kind of gel dyeing apparatus and method are applicable to the polyacrylamide gel dying operation about thick 1mm.
Background technology
By gel electrophoresis (Ago-Gel, polyacrylamide gel) separating bio macromolecular substances (nucleic acid, protein etc.) is technology commonly used in the biological study, along with biology and the interdisciplinary synthesis and ubiquit of environment, these biology techniques in the application in environmentology field also more and more widely and frequent.The laboratory normally uses enamel tray, plastics square box etc. as gel-colored container both at home and abroad at present, in use there is inconvenience in these containers: will directly glue be stripped down from electrophoresis tank or glass plate with hand before the dyeing, be put in the dyeing container, operation one is that consumed time is long like this, the 2nd, the situation of damaged glue is usually arranged, especially very thin polyacrylamide gel usually is difficult to keep its integrality in the process of peeling off; To glue be got in the colouration box with hand or auxiliary thin plate after dyeing finishes and send to observation, doing one like this is operating personnel's too much the contacting with poisonous and hazardous coloring agent material of hand, unfavorable safety, the 2nd, easier damage glue, the 3rd, glue usually stacks or (as thin but also soft polyacrylamide gel not only) in a huddle, observe and cut glue operation etc. for the dyeing of back and all bring very big inconvenience, even sometimes, for glue is launched and badly damaged glue fully, cause experimental result to observe, and merit thanks to one is burst.Present most of experimenter carries out gel-colored operation with skill level by rule of thumb, and middle not by what special equipment consumed time is long, can not guarantee the integrality of glue in dyeing and observation operating process simultaneously fully.According to the domestic and international relevant device device data of look into, only there is the fourth clear spring of Wuhan Virology Institute,Chinan academy of Sciences to invent gel-colored box in 1996, apply for and obtained the utility model patent of invention.But, this gel-colored box mainly is saving dyeing liquor and destainer, can adds, remove dyeing liquor or destainer easily, at object mainly is thickness and all bigger Ago-Gel of hardness, and it is not only terminate in this single operation of dyeing of bubble glue, and strong for not only thin but also soft polyacrylamide gel operability.This patent of invention stops legal effect November 21 calendar year 2001 because of expense.
Summary of the invention
Technical matters to be solved by this invention provides and a kind ofly makes whole gel-colored operating process become simple, a large amount of time can be saved, and can obtain complete beautiful gel-colored figure as a result at last, simultaneously, hand is used gel dyeing apparatus and the method that directly contacts with gel hardly in the operating process.
The technical solution adopted for the present invention to solve the technical problems is: a kind of gel dyeing apparatus is provided, comprise gel-colored container, described gel dyeing apparatus is by the blank chassis in the blank hole that evenly distributes, the detachable blank frame that matches with described blank chassis, the agent set that blank chassis and blank frame constitute, there is the removable corresponding blank film of one deck on blank chassis, and come unstuck container and sealable dyeing container match with agent set and constitutes a whole set of gel dyeing apparatus.
The material of described blank chassis and detachable blank frame is common plastics or organic glass or metal or PVP material; The two blank empty magnitude range is diameter 0.1cm-3.0cm.
Described blank chassis and detachable blank frame are circular or square, circular blank chassis and blank frame range of size are diameter 10cm-30cm, the thick 0.5-3cm of circular base plate, the thick 0.1-1.5cm of blank frame, blank frame altitude range is 0.5cm-4.5cm; Square blank chassis and blank frame range of size length are 10cm-30cm, and width is 10cm-30cm, the square thick 0.5-3cm in blank chassis, and the thick 0.1-1.5cm of square blank frame, square blank frame altitude range is 0.5cm-4.5cm.
Between described blank chassis and the detachable blank frame is to be connected with draw-in groove, and the draw-in groove width range is 0.1-1.5cm, perhaps connects with spiral lamination.
Described corresponding blank film with the chassis, circular film dimensions scope is diameter 12cm-32cm, square film dimensions scope length is 12-32cm, width is 12-32cm, the cavity size that blank cavity is big or small and the chassis is blank is consistent, and the position is corresponding one by one, and membraneous material is as thin as a wafer and transparent plastic material.
The described gel container that comes unstuck adopts organic glass or plastics or PVP plate or enamel or metal material, and container cavity range of size length is 15cm-50cm, and width is 15cm-50cm, highly is 1.0cm-8.0cm.
Described gel-colored container is made up of sealable lid and container cavity, make with lighttight organic glass or plastics or PVP plate or enamel or metal material, its cavity size scope length is 15cm-40cm, and width is 15cm-40cm, highly is 1.0cm-8.0cm.
A kind of gel-colored method comprises the following steps:
(1) adorn an amount of water in the container that comes unstuck, blank film is covered on the blank chassis, make the cavity corresponding one by one good, load onto blank frame, film is fixed on the blank chassis, put into the container that comes unstuck then, water logging does not have;
(2) will contain the electrophoresis tank of glue or glass plate is putting down and is being put into the film top, water logging does not have, slowly rock, the impulse force that utilizes water is peeled off gel on the electrophoresis tank that sticking or glass plate at leisure, take electrophoresis tank or glass plate away from the bottom, gel drops on the film that is covering on the chassis with regard to open and flat, the chassis end is played water spill from the cavity of cavity, chassis and frame;
(3) chassis is being contained glue and be put into together in the dyeing container, behind dyeing 10 ~ 20min, the chassis end is being got up, allowing dyeing liquor spill, the excellent and maintenance deployed condition of glue from the cavity of cavity, chassis and frame;
(4) deliver to gel imaging system and observe and take pictures,, the film of containing glue is connected glue be dragged in the imaging system instrument from the chassis together and get final product as long as frame is taken off.
Beneficial effect
In the whole process, hand has guaranteed that hardly with directly touching gel gel can stand intact, and utilizes the stretching of boehmite gel in liquid, makes it to keep the state that launches always, greatly facilitates taking pictures of back and observes and cut glue work.The application of this device, can save the usual spent time of gel-colored operation widely, can observe and cut work such as glue for follow-up gel great convenience is provided, provide guarantee for obtaining the high gel picture of integrality good quality, operating process has reduced some unnecessary pollutions, more can guarantee operating personnel's safety.
Description of drawings
Fig. 1 is agent set overlay film figure of the present invention.
Fig. 2 is the STRUCTURE DECOMPOSITION synoptic diagram of Fig. 1.
Fig. 3 is the container figure that comes unstuck.
Fig. 4 is dyeing container figure.
Among the figure: the blank chassis of 1-(marginal belt draw-in groove seam), the blank frame of 2-, the blank film of 3-, 4-dyeing container cavity, 5-dyeing container lid.
Embodiment
Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.Should be understood that in addition those skilled in the art can make various changes or modifications the present invention after the content of having read the present invention's instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
As shown in Figure 1, 2, circular blank chassis standard size is diameter 25cm, and detachable blank frame standard size is diameter 25cm, highly is 2.0cm, and is to be connected with draw-in groove between the chassis.Manufacturing materials adopts common plastics or organic glass or metal or PVP material.Do blank membraneous material with preservative film, square, size is long 27cm, wide 27cm.
As shown in Figure 3, the container standard of coming unstuck size is long 40cm, wide 35cm, and height is 4cm.Adopt common organic glass or plastics or PVP plate or enamel or metal material to make.Not with cover.
As shown in Figure 4, gel-colored container, with cover, all be to adopt lighttight organic glass or plastics or PVP plate or enamel or metal material making, standard dimensions is long 35cm, wide 35cm, height is 4cm.
Above each several part plant bulk size all can be made according to gel size specification commonly used.
Its method is: at first, adorn an amount of water in the container that comes unstuck, blank film is covered on the blank chassis, make the cavity corresponding one by one good, load onto blank frame, so just film is fixed on the blank chassis, put into the container that comes unstuck then, water logging does not have; To contain the electrophoresis tank of glue or glass plate is then putting down and is being put into the film top, water logging does not have, slowly rock, the impulse force that utilizes water is peeled off gel on the electrophoresis tank that sticking or glass plate at leisure, take electrophoresis tank or glass plate away from the bottom, gel drops on the film that is covering on the chassis with regard to open and flat, and the end water that gets up in chassis is spilt from the cavity of cavity, chassis and frame; Glue is being contained on the chassis be put into together in the dyeing container, behind dyeing 10 ~ 20min, the chassis end is being got up, allowing dyeing spill from the cavity of cavity, chassis and frame night, glue is excellent and keep deployed condition; Deliver to gel imaging system and observe and take pictures,, the film of containing glue is connected glue be dragged in the imaging system instrument just passable together from the chassis as long as frame is taken off.
Claims (8)
1. gel dyeing apparatus, comprise gel-colored container, it is characterized in that: described gel dyeing apparatus is by the blank chassis (1) in the blank hole that evenly distributes and detachable blank frame (2) the formation agent set that matches with described blank chassis, there is the removable corresponding blank film of one deck (3) on blank chassis, and come unstuck container and sealable gel-colored container match with agent set and constitutes described gel dyeing apparatus.
2. a kind of gel dyeing apparatus according to claim 1 is characterized in that: the material of described blank chassis and detachable blank frame is common plastics or organic glass or metal or PVP material; The two blank empty magnitude range is diameter 0.1cm-3.0cm.
3. a kind of gel dyeing apparatus according to claim 1 and 2, it is characterized in that: described blank chassis and detachable blank frame are for circular or square, circular blank chassis and blank frame range of size are diameter 10cm-30cm, the thick 0.5-3cm of circular base plate, the thick 0.1-1.5cm of blank frame, blank frame altitude range is 0.5cm-4.5cm; Square blank chassis and blank frame range of size length are 10cm-30cm, and width is 10cm-30cm, the square thick 0.5-3cm in blank chassis, and the thick 0.1-1.5cm of square blank frame, square blank frame altitude range is 0.5cm-4.5cm.
4. a kind of gel dyeing apparatus according to claim 3 is characterized in that: between described blank chassis and the detachable blank frame is to be connected with draw-in groove, and the draw-in groove width range is 0.1-1.5cm, perhaps connects with spiral lamination.
5. a kind of gel dyeing apparatus according to claim 1, it is characterized in that: described corresponding blank film with the chassis is for circular or square, circular film dimensions scope is diameter 12cm-32cm, square film dimensions scope length is 12-32cm, width is 12-32cm, the blank cavity size in blank cavity size and chassis is consistent, and position correspondence one by one, and membraneous material is as thin as a wafer and transparent plastic material.
6. a kind of gel dyeing apparatus according to claim 1, it is characterized in that: the described gel container that comes unstuck adopts organic glass or plastics or PVP plate or enamel or metal material, container cavity range of size length is 15cm-50cm, and width is 15cm-50cm, highly is 1.0cm-8.0cm.
7. a kind of gel dyeing apparatus according to claim 1, it is characterized in that: described gel-colored container is made up of sealable lid (5) and container cavity (4), make with lighttight organic glass or plastics or PVP plate or enamel or metal material, its cavity size scope length is 15cm-40cm, width is 15cm-40cm, highly is 1.0cm-8.0cm.
8. a gel-colored method of using the described gel dyeing apparatus of claim 1 comprises the following steps:
(1) adorn an amount of water in the container that comes unstuck, blank film is covered on the blank chassis, make the cavity corresponding one by one good, load onto blank frame, film is fixed on the blank chassis, put into the container that comes unstuck then, water logging does not have;
(2) will contain the electrophoresis tank of glue or glass plate is putting down and is being put into the film top, water logging does not have, slowly rock, the impulse force that utilizes water is peeled off gel on the electrophoresis tank that sticking or glass plate at leisure, take electrophoresis tank or glass plate away from the bottom, gel drops on the film that is covering on the chassis with regard to open and flat, the chassis end is played water spill from the cavity of cavity, chassis and frame;
(3) chassis is being contained glue and be put into together in the dyeing container, behind dyeing 10～20min, the chassis end is being got up, allowing dyeing liquor spill, the excellent and maintenance deployed condition of glue from the cavity of cavity, chassis and frame;
(4) deliver to gel imaging system and observe and take pictures,, the film of containing glue is connected glue be dragged in the imaging system instrument from the chassis together and get final product as long as frame is taken off.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102024217A CN101398353B (en) | 2008-11-07 | 2008-11-07 | Gel dyeing apparatus and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008102024217A CN101398353B (en) | 2008-11-07 | 2008-11-07 | Gel dyeing apparatus and method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101398353A CN101398353A (en) | 2009-04-01 |
| CN101398353B true CN101398353B (en) | 2011-02-09 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008102024217A Expired - Fee Related CN101398353B (en) | 2008-11-07 | 2008-11-07 | Gel dyeing apparatus and method |
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| Country | Link |
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| CN (1) | CN101398353B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103076220A (en) * | 2012-12-29 | 2013-05-01 | 安徽科技学院 | Staining technique for lining film at basin bottom in SRAP molecular markers |
-
2008
- 2008-11-07 CN CN2008102024217A patent/CN101398353B/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103076220A (en) * | 2012-12-29 | 2013-05-01 | 安徽科技学院 | Staining technique for lining film at basin bottom in SRAP molecular markers |
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| Publication number | Publication date |
|---|---|
| CN101398353A (en) | 2009-04-01 |
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Granted publication date: 20110209 Termination date: 20131107 |