CN101332255A - Traditional Chinese medicine extract for preventing avian influenza and preparation method - Google Patents
Traditional Chinese medicine extract for preventing avian influenza and preparation method Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine extract for preventing bird flu and a preparation method thereof. The traditional Chinese medicine extract contains honeysuckle, forsythia, Baikal skullcap root, radix isatidis, thick wood-fern rhizome, heartleaf houtluynia, radix astragali and Bupleurum and has the characteristics of obvious medical effect and safe and convenient use for preventing and curing the bird flu. The preparation method of the invention is that: a. the ingredients are respectively crushed into coarse powders; b. the coarse powders are added with solvent and the extracted liquid is collected; c. the obtained solution is concentrated; d. the clarifying agent is added, fully mixed and stewed; e. the sediments of the clarified liquid medicine are removed by an industrial centrifuge to obtain the liquid medicine that meets the standard. The healing effect of the preparation achieved due to using the preparation method of the invention is exact and the quality is reliable.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition and preparation method thereof, particularly a kind of Chinese medicine extract of birds flu-preventing and preparation method.
Background technology
Bird flu is a kind of acute height lethal infectious disease of the caused birds of A type bird flu virus.Poultry such as chicken, turkey, duck and Carnis Coturnicis japonicae and wild bird, aquatic bird, seabird etc. all can infect, but based on chicken and turkey.Multiple animal and human's classes such as pig, cat, monkey also can infect.
Bird flu occurred in Italy early than 1878, once was called as fowl plague or fowl plague in history.Over more than 100 year, bird flu is constantly being attacked the aviculture of All Around The World, and directly threatens human beings'health and life.1997, the bird flu of Hong Kong outburst caused poultry dead on a large scale, and successively has 18 people to infect bird flu virus, wherein 6 people's death; In December, 2003 to 2004 year March, the H5N1 type bird flu in the Southeast Asia outburst involved nearly all Southeast Asian countries and regions, cause the death of catching an illness of tens million of poultry, and the people infected bird flu virus and death surplus in the of 20.
Though bird flu is found the history in existing more than 100 year, human so far prevention in full force and effect, treatment and the control technology do not grasped as yet.Avian influenza vaccine is the main means of birds flu-preventing outburst.If the vaccine that uses is consistent with the hypotype of the virus of invasion and attack, then vaccine can reach more than 90% the protective rate of poultry.But isolated at present bird flu virus has 15 kinds of H antigens and 9 kinds of N antigens, two kinds of antigenic various combinations can form up to a hundred blood serum subtypes, and popular virus subtype instability, producing " drift ", this brings very big difficulty for the development of vaccine and the diagnosis and the control of bird flu.
Summary of the invention
In view of this, main purpose of the present invention is to provide the Chinese medicine extract and the preparation method of birds flu-preventing, have drug effect characteristics remarkable and safe and convenient to use for the control bird flu, with the preparation determined curative effect that preparation method of the present invention realizes, reliable in quality.
The crude drug of pharmaceutical composition of the present invention is formed and proportioning following (by weight):
Flos Lonicerae 10-100 weight portion Fructus Forsythiae 10-100 weight portion Radix Scutellariae 10-100 weight portion
Radix Isatidis 10-100 weight portion Rhizoma Dryopteris Crassirhizomatis 10-100 weight portion Herba Houttuyniae 10-100 weight portion
Radix Astragali 10-100 weight portion Radix Bupleuri 10-100 weight portion
Preferred side
Flos Lonicerae 25 weight portion Fructus Forsythiaes 25 weight portion Radix Scutellariaes 50 weight portions
Radix Isatidis 25 weight portion Rhizoma Dryopteris Crassirhizomatiss 50 weight portion Herba Houttuyniae 30 weight portions
The Radix Astragali 75 weight portion Radix Bupleuri 30 weight portions
More preferably square
Flos Lonicerae 50 weight portion Fructus Forsythiaes 100 weight portion Radix Scutellariaes 50 weight portions
Radix Isatidis 50 weight portion Rhizoma Dryopteris Crassirhizomatiss 50 weight portion Herba Houttuyniae 30 weight portions
The Radix Astragali 75 weight portion Radix Bupleuri 50 weight portions
The preparation method of this Chinese medicine composition:
A, above eight flavors become coarse powder standby Flos Lonicerae, Fructus Forsythiae, Radix Scutellariae, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Herba Houttuyniae, the Radix Astragali, Radix Bupleuri pulverize separately;
B, coarse powder is put into multi-function extractor, add drinking water and heat dynamic reflux, extract,, decompression cryoconcentration;
C, the solution that obtains squeezed in the economic benefits and social benefits distillations concentration tank concentrate, relative density is not less than 1.05;
D, standard compliant concentrated medicament is added clarifier, clarifier can be chitosan, 101 fruit juice clarifiers or AB clarifier, and can mix use, and adding proportion is 0.01%-5%, fully stirs and leaves standstill after adding clarifier;
E, the medicinal liquid after will clarifying remove precipitate by industrial centrifugal machine and obtain standard compliant medicinal liquid, directly squeeze into 100,000 grades of districts, the volatile oil and 0.1% tween 80 that add to reclaim, adding 0.1% sodium benzoate and 0.05% nipalgin is that the antiseptic direct packaging promptly gets mixture.
In the preparation method of above-mentioned Chinese medicine composition:
The b method is: described coarse powder is put into ultrasonic countercurrent extraction jar extract as solvent with the ethanol water mixed liquid, collect extracting solution;
The e method obtains standard compliant medicinal liquid for the medicinal liquid after will clarifying removes precipitate by industrial centrifugal machine, directly squeezes into 100,000 grades of districts, and adding 0.1% sodium benzoate and 0.05% nipalgin is that the antiseptic direct packaging promptly gets mixture.
In the preparation method of above-mentioned Chinese medicine composition:
The e method is: the medicinal liquid after will clarifying removes precipitate by industrial centrifugal machine and obtains standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, again by 7 parts in medicated powder, white carbon black or add 3 parts of mixing of beneficial powder, spray into volatile oil during total mixing, direct packaging promptly gets soluble powder then.
In the preparation method of above-mentioned Chinese medicine composition:
The e method is: the medicinal liquid after will clarifying removes precipitate by industrial centrifugal machine and obtains standard compliant medicinal liquid, squeezes into the atomization drying workshop, crosses atomization drying equipment and obtains xeraphium, sprays into volatile oil during mixing, and direct packaging promptly gets the concentrated extract powder then.
In the preparation method of above-mentioned Chinese medicine composition:
The e method is: the medicinal liquid after will clarifying removes precipitate by industrial centrifugal machine and obtains standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, press 7 parts in medicine dry powder, 2 parts of sucrose, granulation granulation of 1 part of usefulness of starch then, dry then granulate, spray into volatile oil during total the mixing, direct packaging promptly gets granule then.
The application of described Chinese medicine extract in the medicine of preparation birds flu-preventing.
Present China has completely forbidden antiviral drugs such as amantadine, ribavirin in 2005 and has used in livestock breeding industry, and this has just brought new good development opportunity to Chinese medicine.The Chinese medicine pestilence comprises that disease of viral infection has had the history of two thousand years, has accumulated abundant treatment experience, has summed up many effective side's medicines, as Lonicerae and Forsythiae Powder, SANGJU YIN etc.A large amount of clinical practice and experimentatioies show that antiviral side's medicine is used the treatment of disease of viral infection, has not only received good clinical effectiveness, has also showed the modern science intension of Chinese traditional treatment pestilence to people.According to relevant documents and materials, the application of anti virus herb in clinical, almost spreaded all over domestic present common viral infection disease, as influenza, infectious hepatitis, epidemic encephalitis type B, epidemic hemorrhagic fever, viral myocarditis, viral enteritis, mumps etc.At the beginning of last month of spring in winter in 1998, the northern area influenza is popular, relevant traditional medical unit is dialectical according to theory of Chinese medical science to be exterior cold and interior heat, promptly so-called " fire wrapped by cold ", and drafted Chinese medicine preparation " flu mixture " with certain resisiting influenza virus effect with this, be used to prevent and treat Beijing and surrounding area patient 170,000 people, obtained good result.
The antiviral characteristic and advantage of Chinese medicine is that the treatment by Chinese herbs disease of viral infection is not only evident in efficacy, and because the medicinal property of himself, must show in some characteristics and the advantage that are different from Western medicine aspect the treatment disease of viral infection, mainly show as: the one, at antiviral simultaneously, many medicines have concurrently analgesic, effects such as antiinflammatory, the infection that virus is caused has multiple action, as shorten time of heating, the diffusion that controls inflammation, promote the absorption of inflammation etc., promptly to virus and the viral pathological reaction energy multipath that causes, multi-faceted working is as Flos Lonicerae, Fructus Forsythiae, Herba Houttuyniae etc.; The 2nd, at antiviral simultaneously, the part medicine has the enhancing human body immunity function concurrently, can stop virus to enter cell tissue, as the Radix Astragali, Ganoderma, Radix Panacis Quinquefolii etc.; The 3rd, at antiviral simultaneously, generally seldom injure normal tissue cell, toxic and side effects is less, as Flos Chrysanthemi, Radix Angelicae Sinensis, Radix Polygoni Multiflori etc.; The 4th, be the treatment of individuation because Chinese medicine takes, the state of an illness is had more specific aim, more can accomplish to shoot the arrow at the target.In addition, should see also that because the diversification of middle pharmaceutically active ingredient, virus also seldom develops immunity to drugs to it.Obviously, Chinese medicine has remarkable advantages aspect the treatment disease of viral infection, and has broad application prospects.Chinese medicine has been the inarguable fact to the good efficacy of disease of viral infection, and its antiviral advantage also is obvious to all.
Along with developing rapidly of China's livestock and poultry breeding industry and deepening continuously of veterinary drug research, the application of Chinese medicine on livestock and poultry disease control and prevention is also more and more general, and survival rate and the health level that improves poultry has been played positive role.But as a whole, at present the herbal medicine of China is in also that compatibility of drugs lacks systematic study, product forms is coarse, effective ingredient is imprecise, the low technical level of unstable product quality.Use the research of Chinese medicinal formulae control poultry bird flu and report that seldom do not see the research report that has system deep, the herbal medicine product that bird flu is had fine prevention effect is not seen in veterinary drug market yet.
The present invention at first utilizes the Chinese medical discrimination determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs characteristics that combine with differential diagnosis of diseases, also be the marrow that the traditional Chinese medical science is cured the disease, overall process and various aspects that it is cured the disease through the traditional Chinese medical science, clinical use anti virus herb also must be dialectical, also wanting differential diagnosis of diseases, clearly is to belong to which kind of viral disease, promptly selects medicine with card, with sick medication, organically combine with the differential diagnosis of diseases medication dialectical.
Use antiviral and the integrally-regulated organic conception that combines, specific practice is, antiviral and integrally-regulated combining, using antiviral drugs and using other related drugs to combine.This Therapeutic Method that is conceived to organic conception had both helped disease controlling, also helped the radical cure to viral disease, had both helped the performance of antiviral curative effect, also helped transferring the intrinsic various active factors of body, thus the curative effect of significantly improving.
The mechanism of action of anti virus herb mainly contains the following aspects: the one, directly suppress kill virus; The 2nd, suppress duplicating of virus, check virus multiplication; The 3rd, delay viral caused cytopathy; The 4th, improve the host body immunity function, strengthen the opposing virus capable.The 5th, inducement interferon and the interferon-induced effect of reinforcement.Because the diversification of chemical composition of Chinese materia medica, its antiviral-mechanism may be the result of comprehensive function.
The present invention utilizes above-mentioned differential diagnosis in tcm theory to carry out prescription, antiviral is mainly based on heat-clearing and toxic substances removing, Fang Zhongqian 5 flavor medicine Flos Loniceraes, Fructus Forsythiae, Radix Scutellariae, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis are the key medicine of heat-clearing and toxic substances removing, and the documents and materials proof all has strong inhibitory action to bird flu virus; Each medicine synergism of distinguishing the flavor of, not only strengthened inhibitory action to virus, antibacterial, and the toxicity of single medicinal material and the generation of virus drug resistance have been reduced, in addition according to the administration of suiting the medicine to the illness of the characteristics of influenza morbidity, as alleviating adding Herba Houttuyniae, the Radix Astragali, Radix Bupleuri such as respiratory symptom, hyperpyrexia symptom, human body immunity improving power.Wherein, the Flos Lonicerae master contains chlorogenic acid; The Fructus Forsythiae master contains the forsythin compounds; The Radix Scutellariae master contains baicalin, wogonin; The Radix Isatidis master contains indigo, indirubin; The Rhizoma Dryopteris Crassirhizomatis master contains pannol, the trident phenol of fainting; These compositions are to antibacterial and the viral inhibition activity that all has in various degree.Herba Houttuyniae contains houttuynine sodium bisulfite (volatile ingredient), can reconcile the body nonspecific immunity, can obviously strengthen the phagocytic function of macrophage; The Radix Astragali contains astragalus polysaccharides, and astragalus polysaccharides has significant facilitation to the immunocompetence of humans and animals, can obviously strengthen the phagocytic function of macrophage, increases the TNF secretion, promotes the generation of antiviral antibody; Radix Bupleuri, it is former to contain saikoside, globulariacitrin, Saponin, has the analgesic effect of Heyang.
Specific embodiment
Embodiment 1
Chinese medicine extract of the present invention is made by following raw material medicaments:
Flos Lonicerae 25g Fructus Forsythiae 25g Radix Scutellariae 50g
Radix Isatidis 25g Rhizoma Dryopteris Crassirhizomatis 50g Herba Houttuyniae 30g
Radix Astragali 75g Radix Bupleuri 30g
More than eight flavors be ground into the coarse powder mix homogeneously.
Embodiment 2
Pharmaceutical composition of the present invention is made by following raw material medicaments:
Flos Lonicerae 50g Fructus Forsythiae 100g Radix Scutellariae 50g
Radix Isatidis 50g Rhizoma Dryopteris Crassirhizomatis 50g Herba Houttuyniae 30g
Radix Astragali 75g Radix Bupleuri 50g
More than eight flavors be ground into the coarse powder mix homogeneously.
Embodiment 3
A, take by weighing Flos Lonicerae 25g, Fructus Forsythiae 25g, Radix Scutellariae 50g, Radix Isatidis 25g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 30g are ground into coarse powder;
B, coarse powder are put into multi-function extractor, add drinking water and heat dynamic reflux, extract,, decompression cryoconcentration, collect extracting solution and volatile oil;
C, the solution that obtains squeezed in the economic benefits and social benefits distillations concentration tank concentrate, relative density is not less than 1.05
D, standard compliant concentrated medicament is added clarifier, clarifier can be chitosan, 101 fruit juice clarifiers or AB clarifier, and can mix use, and adding proportion is 0.01%-5%, fully stirs and leaves standstill after adding clarifier;
E, the medicinal liquid after will clarifying remove precipitate by industrial centrifugal machine and obtain standard compliant medicinal liquid, directly squeeze into 100,000 grades of districts, the volatile oil and 0.1% tween 80 that add to reclaim add 0.1% sodium benzoate and 0.05% nipalgin and are the antiseptic direct packaging promptly.
Embodiment 4
The another kind of preparation method of mixture of the present invention comprises the steps:
A, take by weighing Flos Lonicerae 25g, Fructus Forsythiae 25g, Radix Scutellariae 50g, Radix Isatidis 25g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 30g are ground into coarse powder;
B, coarse powder are put into ultrasonic countercurrent extraction jar and are extracted as solvent with the ethanol water mixed liquid, collect extracting solution;
C, the extraction solution that will obtain are squeezed in the economic benefits and social benefits distillation concentration tank and are concentrated, and relative density is not less than 1.05;
D, standard compliant concentrated medicament is added clarifier, clarifier can be chitosan, 101 fruit juice clarifiers or AB clarifier, and can mix use, and adding proportion is 0.01%-5%, fully stirs and leaves standstill after adding clarifier;
E, the medicinal liquid after will clarifying remove precipitate by industrial centrifugal machine and obtain standard compliant medicinal liquid, directly squeeze into 100,000 grades of districts, add 0.1% sodium benzoate and 0.05% nipalgin and be the antiseptic direct packaging promptly.
Embodiment 5
According to embodiment 3 described methods, difference is to select following raw materials in weight for use: Flos Lonicerae 50g, Fructus Forsythiae 100g, Radix Scutellariae 50g, Radix Isatidis 50g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 50g.
Embodiment 6
According to embodiment 4 described methods, difference is to select following raw materials in weight for use: Flos Lonicerae 50g, Fructus Forsythiae 100g, Radix Scutellariae 50g, Radix Isatidis 50g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 50g.
Embodiment 7
The preparation method of soluble powder of the present invention comprises the steps:
A, take by weighing Flos Lonicerae 25g, Fructus Forsythiae 25g, Radix Scutellariae 50g, Radix Isatidis 25g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 30g are ground into coarse powder;
B, coarse powder are put into multi-function extractor, add drinking water and heat dynamic reflux, extract,, decompression cryoconcentration, collect extracting solution and volatile oil;
C, the solution that obtains squeezed in the economic benefits and social benefits distillations concentration tank concentrate, relative density is not less than 1.05;
D, standard compliant concentrated medicament is added clarifier, clarifier can be chitosan, 101 fruit juice clarifiers or AB clarifier, and can mix use, and adding proportion is 0.01%-5%, fully stirs and leaves standstill after adding clarifier;
E, the medicinal liquid after will clarifying remove precipitate by industrial centrifugal machine and obtain standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, again by 7 parts in medicated powder, white carbon black or add 3 parts of mixing of beneficial powder, spray into volatile oil during total mixing, direct packaging promptly then.
Embodiment 8
The another kind of preparation method of soluble powder of the present invention comprises the steps:
A, take by weighing Flos Lonicerae 25g, Fructus Forsythiae 25g, Radix Scutellariae 50g, Radix Isatidis 25g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 30g are ground into coarse powder;
B, coarse powder are put into ultrasonic countercurrent extraction jar and are extracted as solvent with the ethanol water mixed liquid, collect extracting solution;
C, the solution that obtains squeezed in the economic benefits and social benefits distillations concentration tank concentrate, relative density is not less than 1.05;
D, standard compliant concentrated medicament is added clarifier, clarifier can be chitosan, 101 fruit juice clarifiers or AB clarifier, and can mix use, and adding proportion is 0.01%-5%, fully stirs and leaves standstill after adding clarifier;
E, the medicinal liquid after will clarifying remove precipitate by industrial centrifugal machine and obtain standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, and again by 7 parts in medicated powder, white carbon black or add 3 parts of mixing of beneficial powder, direct packaging promptly then.
Embodiment 9
According to embodiment 7 described methods, difference is to select following raw materials in weight for use: Flos Lonicerae 50g, Fructus Forsythiae 100g, Radix Scutellariae 50g, Radix Isatidis 50g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 50g.
Embodiment 10
According to embodiment 8 described methods, difference is to select following raw materials in weight for use: Flos Lonicerae 50g, Fructus Forsythiae 100g, Radix Scutellariae 50g, Radix Isatidis 50g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 50g.
Embodiment 11
The preparation method of concentrated extract powder of the present invention comprises the steps:
A, take by weighing Flos Lonicerae 25g, Fructus Forsythiae 25g, Radix Scutellariae 50g, Radix Isatidis 25g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 30g are ground into coarse powder;
B, coarse powder are put into multi-function extractor, add drinking water and heat dynamic reflux, extract,, decompression cryoconcentration, collect extracting solution and volatile oil;
C, the solution that obtains squeezed in the economic benefits and social benefits distillations concentration tank concentrate, relative density is not less than 1.05;
D, standard compliant concentrated medicament is added clarifier, clarifier can be chitosan, 101 fruit juice clarifiers or AB clarifier, and can mix use, and adding proportion is 0.01%-5%, fully stirs and leaves standstill after adding clarifier;
E, the medicinal liquid after will clarifying remove precipitate by industrial centrifugal machine and obtain standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, spray into volatile oil during mixing, and direct packaging promptly then.
Embodiment 12
The another kind of preparation method of concentrated extract powder of the present invention comprises the steps:
A, take by weighing Flos Lonicerae 25g, Fructus Forsythiae 25g, Radix Scutellariae 50g, Radix Isatidis 25g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 30g are ground into coarse powder;
B, coarse powder are put into ultrasonic countercurrent extraction jar and are extracted as solvent with the ethanol water mixed liquid, collect extracting solution;
C, the solution that obtains squeezed in the economic benefits and social benefits distillations concentration tank concentrate, relative density is not less than 1.05;
D, standard compliant concentrated medicament is added clarifier, clarifier can be chitosan, 101 fruit juice clarifiers or AB clarifier, and can mix use, and adding proportion is 0.01%-5%, fully stirs and leaves standstill after adding clarifier;
E, the medicinal liquid after will clarifying remove precipitate by industrial centrifugal machine and obtain standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, and direct packaging promptly then.
Embodiment 13
According to embodiment 11 described methods, difference is to select following raw materials in weight Flos Lonicerae 50g, Fructus Forsythiae 100g, Radix Scutellariae 50g, Radix Isatidis 50g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 50g for use.
Embodiment 14
According to embodiment 12 described methods, difference is to select following raw materials in weight for use: Flos Lonicerae 50g, Fructus Forsythiae 100g, Radix Scutellariae 50g, Radix Isatidis 50g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 50g.
Embodiment 15
The preparation method of granule of the present invention comprises the steps:
A, take by weighing Flos Lonicerae 25g, Fructus Forsythiae 25g, Radix Scutellariae 50g, Radix Isatidis 25g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 30g are ground into coarse powder;
B, coarse powder are put into multi-function extractor, add drinking water and heat dynamic reflux, extract,, decompression cryoconcentration, collect extracting solution and volatile oil;
C, the solution that obtains squeezed in the economic benefits and social benefits distillations concentration tank concentrate, relative density is not less than 1.05;
D, standard compliant concentrated medicament is added clarifier, clarifier can be chitosan, 101 fruit juice clarifiers or AB clarifier, and can mix use, and adding proportion is 0.01%-5%, fully stirs and leaves standstill after adding clarifier;
E, the medicinal liquid after will clarifying remove precipitate by industrial centrifugal machine and obtain standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, press 7 parts in medicine dry powder, 2 parts of sucrose, granulation granulation of 1 part of usefulness of starch then, dry then granulate, spray into volatile oil during total the mixing, direct packaging promptly then.
Embodiment 16
The another kind of preparation method of granule of the present invention comprises the steps:
A, take by weighing Flos Lonicerae 25g, Fructus Forsythiae 25g, Radix Scutellariae 50g, Radix Isatidis 25g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 30g are ground into coarse powder;
B, coarse powder are put into ultrasonic countercurrent extraction jar and are extracted as solvent with the ethanol water mixed liquid, collect extracting solution;
C, the solution that obtains squeezed in the economic benefits and social benefits distillations concentration tank concentrate, relative density is not less than 1.05;
D, standard compliant concentrated medicament is added clarifier, clarifier can be chitosan, 101 fruit juice clarifiers or AB clarifier, and can mix use, and adding proportion is 0.01%-5%, fully stirs and leaves standstill after adding clarifier;
E, the medicinal liquid after will clarifying remove precipitate by industrial centrifugal machine and obtain standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, press 7 parts in medicine dry powder, 2 parts of sucrose, granulation granulation of 1 part of usefulness of starch then, dry, granulate, total contaminated product, direct packaging promptly then.
Embodiment 17
According to embodiment 15 described methods, difference is to select following raw materials in weight for use: Flos Lonicerae 50g, Fructus Forsythiae 100g, Radix Scutellariae 50g, Radix Isatidis 50g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 50g.
Embodiment 18
According to embodiment 16 described methods, difference is to select following raw materials in weight for use: Flos Lonicerae 50g, Fructus Forsythiae 100g, Radix Scutellariae 50g, Radix Isatidis 50g, Rhizoma Dryopteris Crassirhizomatis 50g, Herba Houttuyniae 30g, Radix Astragali 75g, Radix Bupleuri 50g.
The consumption of Chinese medicine extract birds flu-preventing of the present invention is respectively:
(1) mixture is used to prevent chicken, duck bird flu, mixes drink (calculating with 24 hours amounts of drinking water of poultry), and every 1L water adds 4-6ml; Be used for the treatment of chicken, duck bird flu, mix drink: every 1L water adds 6-12ml, logotype 3-5 day, also whole day amount medicine can be divided sooner or later and feed for twice.
(2) soluble powder is used to prevent chicken, duck bird flu, mixes drink (calculating with 24 hours amounts of drinking water of poultry), and every 1L water adds 4-6g; Be used for the treatment of chicken, duck bird flu, mix drink: every 1L water adds 6-12g, logotype 3-5 day, also whole day amount medicine can be divided sooner or later and feed for twice.
(3) the concentrated extract powder is used to prevent chicken, duck bird flu, mixes drink (calculating with 24 hours amounts of drinking water of poultry), and every 1L water adds 2-3g; Be used for the treatment of chicken, duck bird flu, mix drink: every 1L water adds 3-6g, logotype 3-5 day, also whole day amount medicine can be divided sooner or later and feed for twice
(4) dissolved granule is used for chicken, duck bird flu, mixes drink (calculating with 24 hours amounts of drinking water of poultry), and every 1L water adds 4-6g; Be used for the treatment of chicken, duck bird flu, mix drink: every 1L water adds 6-12g, logotype 3-5 day, also whole day amount medicine can be divided sooner or later and feed for twice
Below further specify the Chinese medicine extract of birds flu-preventing of the present invention in clinical prevention effect by the animal experiment example to the bird flu chicken.
Test: the bird flu test is prevented, controlled to the Chinese medicine extraction compositions
1.1, materials and methods
1.1 experimental animal and grouping
1.1.1 test material: SPF chicken SPF Embryo Gallus domesticus is respectively available from laboratory animal research center, Beijing and China Veterinary Drugs Supervisory Inst.; Used Chinese crude drug raw material is bought from medical material company; Baoding Jizhong Pharmaceutical Co., Ltd. extracts and preparation Chinese medicine composition drinking agent (oral liquid).1 milliliter of oral liquid is equivalent to the 1g crude drug; The counteracting toxic substances strain: (half infects concentration 1.0*10 to H9 blood serum subtype bird flu virus
-6), Agricultural University Of Hebei veterinary college fowl diseases research department preserves.
1.1.2 grouping: prevent 1 group to prevent 2 groups to treat 1 group and treat 2 groups of matched groups
1.2 experimental design and method
1.1.1 laboratory animal is selected and environment control
Select the SPF chicken of 49 age in days health for use, the group observes 48h to chicken, and the chicken group mental status, feces, feed intake and drinking-water all just often begin test.In advance test is carried out fumigation with room, incubator, cage tool etc., indoor being controlled at about 20 ℃ keeps good ventilation, and it is negative to adopt hemagglutination inhibition test (HI test) (H) to measure bird flu (AIV).
1.1.2 experimental technique
Get 20 pieces of 8 age in days SPF Embryo Gallus domesticus and float egg transportation, 9 ages in days inoculation bird flu H9 (blood serum subtype) virus, it is standby to gather in the crops ℃ preservation of the 48h～96h allantoic fluid-20.
The chick embryo allantoic liquid thin liquid that will contain bird flu virus is 0.5*10
-5Concentration, every chicken abdominal muscles injection 0.2ml inoculation.
Laboratory animal is divided into 5 groups (seeing Table 1) at random: prevent 1 group for low dose of prevention, carry out oral administration medicine supplying by the 0.5ml/kg body weight; Prevent 2 groups for requiring dosage, carry out oral administration medicine supplying by the 1.0ml/kg body weight; Two prevention groups were all offerd medicine 3 days continuously.After 48 hours four groups of chickens are inoculated every chicken abdominal muscles injection 0.2ml inoculation simultaneously at interval, according to the protective rate of two prevention groups of incidence statistics; Begin after the treatment group morbidity the dispensing of treatment group oral administration, treat 1 group and undertaken, treat 2 groups of 1ml/kg by carrying out by body weight 1ml/kg.Treatment time is 5 days.
1.1.3 test data sheet and data statistics:
Table 1 test grouping
Annotate: ∨ represents to carry out this operation, ▲ expression counteracting toxic substances
The evaluating drug effect standard: observe death condition and the clinical manifestation of chicken every day, the chicken that dies of illness carries out necropsy, and every chicken is weighed before and after the test.By following standard drug effect is advanced to estimate: (1) death: all at duration of test, bird flu classical symptom and dead appears, and it is dead that necropsy has promptly being judged to be of typical cytopathic feature to infect.(2) effective: after the off-test, all after administration still chickens of survival all be judged to be effectively, the percentage rate that every group of chicken number of surviving accounts for the test chicken number during off-test is an effective percentage.(3) weightening finish: the body weight of every chicken during according to on-test and during off-test, calculate the average weight and the standard deviation of the weightening finish of every chicken, every group of test chicken.The relative weight gain rate is to calculate by the ratio of each medication treatment group with the average weight gain of normal healthy controls group, and wherein the normal healthy controls group is made as 100%.
2. result and analysis
2.1 prevention group
1 group of preventive dosage and 2 groups from dispensing next day visible spirit show excited slightly, other no abnormality seen.After the counteracting toxic substances: 24h observes does not have new the variation; Concrete outcome sees the following form 2.
2.2 treatment
After the counteracting toxic substances: 24h observes does not have new the variation; Matched group was dead 1 in the 48th hour, and other most performance spirit are tired, and green loose stool is arranged, then dispensing treatment immediately, concrete outcome spare following table 2.
The record of table 2 survival number scale
3. discuss
Comprehensive above experimental result, Chinese medicine extract of the present invention has good protection effect to the chicken of artificial challenge's bird flu virus, and the survival rate that infects chicken reaches 95%, and non-high pathogenic avian influenza has the excellent prevention effect; Strengthen using dosage for the chicken group who has fallen ill, still have excellent curative.
The above is preferred embodiment of the present invention only, is not to be used to limit protection scope of the present invention.
Claims (9)
1, a kind of Chinese medicine extract of birds flu-preventing is characterized in that this Chinese medicine extract made by following raw material medicaments:
Flos Lonicerae 10-100 weight portion Fructus Forsythiae 10-100 weight portion Radix Scutellariae 10-100 weight portion
Radix Isatidis 10-100 weight portion Rhizoma Dryopteris Crassirhizomatis 10-100 weight portion Herba Houttuyniae 10-100 weight portion
Radix Astragali 10-100 weight portion Radix Bupleuri 10-100 weight portion.
2, Chinese medicine extract as claimed in claim 1 is characterized in that this Chinese medicine extract made by following raw material:
Flos Lonicerae 25 weight portion Fructus Forsythiaes 25 weight portion Radix Scutellariaes 50 weight portions
Radix Isatidis 25 weight portion Rhizoma Dryopteris Crassirhizomatiss 50 weight portion Herba Houttuyniae 30 weight portions
The Radix Astragali 75 weight portion Radix Bupleuri 30 weight portions.
3, Chinese medicine extract as claimed in claim 1 is characterized in that this Chinese medicine extract made by following raw material:
Flos Lonicerae 50 weight portion Fructus Forsythiaes 100 weight portion Radix Scutellariaes 50 weight portions
Radix Isatidis 50 weight portion Rhizoma Dryopteris Crassirhizomatiss 50 weight portion Herba Houttuyniae 30 weight portions
The Radix Astragali 75 weight portion Radix Bupleuri 50 weight portions.
4, a kind of preparation method that is used for the Chinese medicine extract of birds flu-preventing is characterized in that this method is:
Flos Lonicerae 10-100 weight portion Fructus Forsythiae 10-100 weight portion Radix Scutellariae 10-100 weight portion
Radix Isatidis 10-100 weight portion Rhizoma Dryopteris Crassirhizomatis 10-100 weight portion Herba Houttuyniae 10-100 weight portion
Radix Astragali 10-100 weight portion Radix Bupleuri 10-100 weight portion
A, above eight flavors become direct packaging behind the standby or mixing of coarse powder with Flos Lonicerae, Fructus Forsythiae, Radix Scutellariae, Radix Isatidis, Rhizoma Dryopteris Crassirhizomatis, Herba Houttuyniae, the Radix Astragali, Radix Bupleuri pulverize separately;
B, get described coarse powder and put into multi-function extractor, add drinking water and heat dynamic reflux, extract,, the decompression cryoconcentration is collected extracting solution and volatile oil;
C, the solution that obtains squeezed in the economic benefits and social benefits distillations concentration tank concentrate, relative density is not less than 1.05;
D, standard compliant concentrated medicament is added clarifier, clarifier is chitosan, 101 fruit juice clarifiers or AB clarifier, and can mix use, and adding proportion is 0.01%-5%, fully stirs and leaves standstill after adding clarifier;
E, the medicinal liquid after will clarifying remove precipitate by industrial centrifugal machine and obtain standard compliant medicinal liquid, directly squeeze into 100,000 grades of districts, the volatile oil and 0.1% tween 80 that add to reclaim, adding 0.1% sodium benzoate and 0.05% nipalgin is that the antiseptic direct packaging promptly gets mixture.
5, preparation method according to claim 4 is characterized in that the b method in this method is:
Described coarse powder is put into ultrasonic countercurrent extraction jar extract as solvent, collect extracting solution with the ethanol water mixed liquid;
The e method is: the medicinal liquid after will clarifying removes precipitate by industrial centrifugal machine and obtains standard compliant medicinal liquid.Directly squeeze into 100,000 grades of districts, adding 0.1% sodium benzoate and 0.05% nipalgin is that the antiseptic direct packaging promptly gets mixture.
6,, it is characterized in that the e method in this method is according to claim 4 or 5 described preparation methoies:
Medicinal liquid after the clarification is removed precipitate by industrial centrifugal machine obtain standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, again by 7 parts in medicated powder, white carbon black or add 3 parts of mixing of beneficial powder, spray into volatile oil during total mixing, direct packaging promptly gets soluble powder then.
7,, it is characterized in that the e method in this method is according to claim 4 or 5 described preparation methoies:
Medicinal liquid after the clarification is removed precipitate by industrial centrifugal machine obtain standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, spray into volatile oil during mixing, direct packaging promptly gets the concentrated extract powder then.
8,, it is characterized in that the e method in this method is according to claim 4 or 5 described preparation methoies:
Medicinal liquid after the clarification is removed precipitate by industrial centrifugal machine obtain standard compliant medicinal liquid, squeeze into the atomization drying workshop, cross atomization drying equipment and obtain xeraphium, press 7 parts in medicine dry powder, 2 parts of sucrose, granulation granulation of 1 part of usefulness of starch then, dry then granulate, spray into volatile oil during total the mixing, direct packaging promptly gets granule then.
9, as the application of any one described Chinese medicine extract among the claim 1-3 in the medicine of preparation birds flu-preventing.
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| CN108576427A (en) * | 2018-03-02 | 2018-09-28 | 安徽宏亮饲料科技有限公司 | A kind of additive and preparation method thereof preventing small swine flu |
| CN108713641A (en) * | 2018-05-08 | 2018-10-30 | 广东海洋大学 | A kind of chitosan quaternary ammonium salt and Chinese herbal composite feed additive and its preparation method and application |
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