CN101314755B - Partioning hybridization reaction device for biological chip - Google Patents
Partioning hybridization reaction device for biological chip Download PDFInfo
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- CN101314755B CN101314755B CN 200710028282 CN200710028282A CN101314755B CN 101314755 B CN101314755 B CN 101314755B CN 200710028282 CN200710028282 CN 200710028282 CN 200710028282 A CN200710028282 A CN 200710028282A CN 101314755 B CN101314755 B CN 101314755B
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Abstract
The invention relates to a biochip hybridization sectioning device which is a fence special for the biochip, particularly a sectioning device which is arranged on a fence so as to facilitate scanning and high-flux sample-application detecting. The device is suitable for the hybridization reaction sectioning arrangement of all the biochips that adopt a slide as the carrier; and the biochip hybridization sectioning device has the remarkable advantages over the conventional design and device in scanning simplicity and rapidness, the flux of the hybridization reaction operation and the automation.
Description
Affiliated technical field
The present invention relates to a kind of biochip hybridization partitioning device, particularly may command biochip hybridization conversion zone and the partitioning device being convenient to scan.
Technical background
Biochip is meant the microarray of highdensity tissue, cell, protein, nucleic acid, carbohydrate and other biological components on the solid supports such as being coated on slide, silicon chip, nylon membrane.The sample of chip and mark is hybridized, and can realize the analysis to biological sample through detecting hybridization signal.Biochip technology through adopting technology such as microelectronics, micromechanics, special chemical treatment, bio operon and process integration in chip, makes it serialization, integrated and microminiaturized with many discontinuous analytic processes (like specimen preparation, chemical reaction and analyzing and testing).Biochip appear as the mankind provide can carry out at a high speed individual bioinformation, the strong technique means of parallel acquisition and analysis, so will become important information collection and the processing platform in the following information biology research.
At present, biochip the most commonly used is to be the biochip of carrier with the slide glass.In actual the use, liquid is not excessive when guaranteeing hybridization, perhaps guarantees between a plurality of hybridization regions on the same carrier slide separately, add the ready-formed fence in slide surface.Yet, from present commonly used be that the constitutional features of the biochip of carrier sees that there is disadvantage in said fence with the slide glass:
After hybridization is accomplished, need remove fence and could chip be put on the scanner and scan because fence has certain thickness and retractility, and chip contact the back variation in thickness with its screens indefinite, so be difficult to definite focal length that scans, cause scanning the image distortion that obtains.In this case, only remove fence, screens is directly contacted with the rigidity slide, could accurately set the scanning focal length.Yet the operation of removing fence is complicated, and the operation of palpus craftization, thereby has influenced the robotization of instrument.In addition, also residual because of the viscous adhesive pledge usually causes the background of scan image to cross strong or dirty.On the other hand, because the existence of the more random fence in position,, cause application of sample speed slowly and be dissolved in and go wrong so can only use single head pipettor application of sample during operation.These factors undoubtedly the day of one's doom simple and directization, robotization and the high-throughput of restriction biochip on using.
Therefore, be necessary the structure of existing chip apparatus is carried out necessary improvement.
Summary of the invention
In order to have solved the problems referred to above, the design people has designed the special-purpose fence of a kind of biochip, and this design can directly scan reacted hybridization sample not removing under the fence condition.Simultaneously, the design of the hybridization region of fence is fit to 8 road pipettor application of samples of standard, thereby makes the hybridization and simple to operationization of signal scanning of biochip, so as to improving the automatization level and the high-throughput performance of biochip test.
Therefore, an object of the present invention is to provide a kind of is the biochip private partition hybridization device of carrier with the slide glass, and this device is provided with scanner screens point of suppon on fence, and on fence, is provided with the hybridization district of 8 row hollow outs.
According to a preferred embodiment of the invention, wherein scanner screens point of suppon is three vacancy sections that are triangle arrangement, and the position of vacancy section is just in time corresponding with the point of contact of scanner slide screens.
According to a preferred embodiment of the invention; The special-purpose hybridization of wherein said biochip partitioning device; It is characterized in that scanner screens point of suppon is three vacancy sections, the shape of each vacancy section can be Any shape such as circular, oval, square, trilateral.Area can be 1mm
2Or more than, make the screens point of contact of scanner just in time be positioned at vacancy section;
According to another preferred embodiment of the present invention; 8 row vacancy section hybridization districts wherein; Spacing is corresponding between the road of spacing and the standard 8 road pipettors of 8 row reaction zones; Can want corresponding in the hybridization district but each road suction nozzle of 8 road pipettors is sharp over against should also certain skew being arranged at the center;
According to another preferred embodiment of the present invention, wherein can there be 8 row, 2 row, multiple columns such as 8 row, 3 row in the hybridization district.Each reaction zone can be multiple shapes such as rectangle, circle, ellipse.
According to another preferred embodiment of the present invention; The material of wherein said fence has the single face non-setting adhesive; Can seamlessly stick on the surface of slide glass, the material of fence can be plastics, rubber, the various materials of polymkeric substance, and thickness can change at 0.05～5mm;
Describe static structure, principle of work and the dynamic structure of apparatus of the present invention in detail below in conjunction with accompanying drawing.
Shown in accompanying drawing 1, said fence is designed to the scanner screens point of suppon of three vacancy sections (1), and vacancy section is triangle arrangement, and the position is just in time corresponding with the point of contact of scanner slide screens.Wherein each vacancy section can be Any shape such as circular, oval, square, trilateral.Its area is approximately 1mm
2Or 1mm
2More than, can make the screens point of contact of scanner just in time be positioned at vacancy section like this.Based on this structure; The biochip that has fence is when scanning, and the point of contact of instrument screens is in direct contact with the slide rigid surface, rather than the fence surface; Thereby can make scanning that stable focusing is arranged, avoid manual the throw off trouble of fence film and the left local residual trace behind the striping simultaneously.
Can be found out that by accompanying drawing 2 described fence is designed with 8 row vacancy sections (being the hybridization district) (2), spacing is corresponding just between the road of spacing and the standard 8 road pipettors (3) of 8 row reaction zones, can be over against should be at the center.Though also certain skew can be arranged sometimes, each road suction nozzle point of 8 road pipettors wants corresponding in the hybridization district.Because the diplopore spacing of the pitch of holes of standard 8 road pipettor track pitches and 96 orifice plates, the tube spacing of 8 connecting legs and 384 orifice plates is all corresponding, therefore, 8 row reaction intervals distances of said fence are also corresponding with above-mentioned hole tube spacing.With 8 row's pipettors is instrument, can between above-mentioned vessel and biochip, carry out 8 road bulk liquid and move.Can there be 8 row, 2 row, multiple columns such as 8 row, 3 row in the hybridization district.Each reaction zone can be multiple shapes such as rectangle, circle, ellipse.Based on this constitutional features, can make of the integral way operation of biochip hybridization operation with 8 passages, improved 8 times than traditional single pipettor single passage operation detection flux.Simultaneously,, also make application of sample be adapted at moving automatically on the liquid workplatform and carry out, thereby improved the level of automation of biochip hybridization reaction because the stdn reaction zone designs.
The material of said fence has the single face non-setting adhesive, can seamlessly stick on the surface of slide glass.The material of fence can be any material that is selected from plastics, rubber, polymkeric substance, and its thickness can change between 0.05～5mm.
Biochip hybridization partitioning device of the present invention is applicable to that all are the biochip of carrier with the slide glass, comprise the hybridization subregion of gene chip, protein chip, cell chip, organization chip, carbohydrate chip etc.This device makes the hybridization and simple to operationization of signal scanning of biochip, thereby has improved the automatization level and the high-throughput performance of biochip test greatly.Aspect the flux and robotization of the simple and direct property that scans, hybridization operation, compare with device with traditional design, the present invention has significant advantage and technical progress.
Description of drawings
Fig. 1 fence hollow out screens point of suppon synoptic diagram
Fig. 2 fence 8 row hybridization subregions and and 8 road pipettor suction nozzles between the spacing corresponding relation
Embodiment
Embodiment 1, space-sharing hybridization reaction device for biological chip
Shown in Fig. 1-2; The present invention is that a kind of specializing in the slide glass is the space-sharing hybridization reaction fence that the biochip of carrier is used; The scanner screens point of suppon (1) of three hollow outs of fence design, the shape of each vacancy section is circular, oval, square, leg-of-mutton, area is 1mm greatly
2Or 1mm
2More than.During operation, should make the screens point of contact of scanner just in time aim at vacancy section (1).The biochip that has a hollow out point of suppon fence just can directly scan under the condition that does not remove film, and can obtain stable focusing when scanning, has avoided the manual problem that leaves residual trace after taking off the trouble of film and taking off film simultaneously.
Fence also has 8 row hollow out hybridization districts (2); The diplopore spacing of the pitch of holes of spacing and standard 8 road pipettors (3), 96 orifice plates of 8 row reaction zones, the tube spacing of 8 connecting legs and 384 orifice plates is all corresponding; Can be instrument so just with 8 row's pipettors; Between above-mentioned vessel and biochip, carry out 8 road bulk liquid and move, and can move on the liquid workplatform in robotization and operate.Can there be 8 row, 2 row, multiple columns such as 8 row, 3 row in the hybridization district.Reaction zone can be rectangle, circle or elliptical shape.So design can make the biochip hybridization operation carry out with the integral way of 8 passages, improves 8 times than traditional single pipettor single passage operation detection flux.
The material of fence has the single face non-setting adhesive, the seamless surface that sticks on slide glass, and the material of fence can be various materials such as plastics, rubber, polymkeric substance, thickness can change at 0.05～5mm.
The method of use of embodiment 2, space-sharing hybridization reaction device for biological chip
The use of this reaction unit is following:
On through the slide glass after the chemical conversion treatment, paste subregion fence of the present invention, and compress and do not stay the slit.Then, use spotting robot point in each hybridization region to add micro probe array, and room temperature placed 12 hours, so that probe and slide Chemical bond.Dry up subsequent use behind the unconjugated probe of use washings flush away.Prepare hybridization sample template, 8 one group, template is placed in 96 orifice plates or 384 orifice plates or 8 connecting legs.Use 8 road pipettors, 8 one group of application of samples of masterplate in the chip hybridization district, are added hybridization solution by 8 one group more in batches.Chip is placed on the automatic hybridization instrument, sets hybridization temperature, hybridization time, washing times, washing time, beginning hybridization and washing.Behind the EO, take out chip, directly be placed on together with fence in the screens of scanner, it is corresponding good with the screens vacancy section of fence with the point of contact of screens to note.After setting sweep parameter, scan, read data and analytical results.
Claims (1)
1. one kind is the hybridization subregion fence device of the biochip of carrier with the slide glass; It is characterized in that this device is provided with scanner screens point of suppon on fence; And on fence, be provided with the hybridization district of 8 row hollow outs; Scanner screens point of suppon is three vacancy sections that are triangle arrangement; And the position of vacancy section is just in time corresponding with the point of contact of scanner slide screens, and point of suppon vacancy section and hybridization district are selected from circle, ellipse, square, triangular shaped, and the area of point of suppon vacancy section is approximately 1mm
2
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710028282 CN101314755B (en) | 2007-05-30 | 2007-05-30 | Partioning hybridization reaction device for biological chip |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710028282 CN101314755B (en) | 2007-05-30 | 2007-05-30 | Partioning hybridization reaction device for biological chip |
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| CN101314755A CN101314755A (en) | 2008-12-03 |
| CN101314755B true CN101314755B (en) | 2012-05-30 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101942385B (en) * | 2009-07-06 | 2013-08-14 | 中山大学达安基因股份有限公司 | Full-automatic hybridization oven and implementation method and application thereof |
| CN104614517B (en) * | 2014-01-09 | 2016-03-02 | 南通大学 | The using method of dismountable microslide couveuse |
| CN107632384A (en) * | 2017-10-26 | 2018-01-26 | 桂林优利特医疗电子有限公司 | Flange slide and anti-dry cover glass and wire frame preparation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580753A (en) * | 2003-08-01 | 2005-02-16 | 北京博奥生物芯片有限责任公司 | Micro array reaction unit and its use |
| JP2005218439A (en) * | 2004-02-06 | 2005-08-18 | Dnaform:Kk | Chip for detecting nucleic acid |
| CN2762136Y (en) * | 2005-01-18 | 2006-03-01 | 中国人民解放军军事医学科学院放射医学研究所 | Electrochemical array sensing chip |
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2007
- 2007-05-30 CN CN 200710028282 patent/CN101314755B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580753A (en) * | 2003-08-01 | 2005-02-16 | 北京博奥生物芯片有限责任公司 | Micro array reaction unit and its use |
| JP2005218439A (en) * | 2004-02-06 | 2005-08-18 | Dnaform:Kk | Chip for detecting nucleic acid |
| CN2762136Y (en) * | 2005-01-18 | 2006-03-01 | 中国人民解放军军事医学科学院放射医学研究所 | Electrochemical array sensing chip |
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Address after: 510665 No. 19 incense Hill Road, hi tech Industrial Development Zone, Guangdong, Guangzhou Patentee after: Guangzhou Da'an gene Co.,Ltd. Address before: 510665 No. 19 incense Hill Road, hi tech Industrial Development Zone, Guangdong, Guangzhou Patentee before: DA AN GENE CO., LTD. OF SUN YAT-SEN University |
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