CN107632384A - Flange slide and anti-dry cover glass and wire frame preparation method - Google Patents
Flange slide and anti-dry cover glass and wire frame preparation method Download PDFInfo
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- CN107632384A CN107632384A CN201711021345.5A CN201711021345A CN107632384A CN 107632384 A CN107632384 A CN 107632384A CN 201711021345 A CN201711021345 A CN 201711021345A CN 107632384 A CN107632384 A CN 107632384A
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- wire frame
- slide
- cover glass
- lamellar body
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Abstract
The invention discloses a kind of flange slide and prevent dry cover glass and wire frame preparation method.Flange slide includes rectangle lamellar body, rectangle lamellar body front is provided with single or more than two rectangles, circle, obround elements lattice and dyed for placing histotomy, each unit lattice are in the upper raised wire frame in lamellar body front, hydrophobic glass ink can be used to be made by way of forming wire frame after silk-screen printing, baking and curing, also can be made of the mode for the hydrophobic glue-film stickup formation wire frame for being coated with PUR or adhesive sticker being punched, or the mode rule using curable glue dispensing is made；The anti-dry cover glass being used cooperatively with slide, its area can be suitable with slide or suitable with the outer area of maximum raised wire frame on slide, prevent the airtight cavity that one gap of formation is consistent between dry cover glass and flange slide projection wire frame.The present invention can prevent reagent outflow, can save reagent and reduce cost, can prevent tissue dry plate.
Description
Technical field
The present invention relates to biology, medical treatment detection utensil is related to, specially a kind of flange for tissue section strain carries
Slide and anti-dry cover glass and wire frame preparation method.
Background technology
Biology with medical test, for the cell of tissues observed sample, the Histological Study of subcellsular level, often
Need to dye the material (such as human body, animal, plant biological tissue section) being attached on slide.Traditional way
It is that first the histotomy on slide is enclosed to form a ring of encirclement, then with special wax crayon before (after dewaxing) dyeing
By reagent dropwise in this ring of encirclement, after being incubated a period of time, incubation reagent is rinsed well, then a kind of reagent is added dropwise down, then
It is incubated.
The experiment of one dyeing may include multiple reagent dropwise, incubation, flushing process.Some reagents will be incubated longer
Time, in order that reagent is not vaporized dry and formed in the direct exposure air that causes to cut into slices " dry in long-time incubation period
Piece " phenomenon, conventional special wax crayon enclose histotomy to come, and reagent can be prevented to be lost in, and reduce the area of reagent covering, but
Only also dry plate is prevented with special crayon drawing circle, it is necessary to much more extra be added dropwise some reagents, or the slide after reagent will be added dropwise
It is put into wet box and is incubated, the ink of the anti-reagent evaporation of one layer of oil droplet formation is either added dropwise on reagent is incubated or using a kind of
Structure with cover glass neck, whole dyeing, flushing, incubation are all carried out in the gap neck that slide is formed with cover glass.
In SABC test, particularly during full-automatic immunohistochemical staining, pathology that typically all needs are dyed
Section is attached on slide, and reagent is added dropwise using one kind in above-mentioned anti-dry measure, after being incubated, passes through specific dye
Color identifies and distinguishes between specific cell and its pathological change feature.
In automated staining system, if mentioned reagent is completely covered the reagent area of slide, and at 20~30 points
Not dry plate (because reagent evaporation makes pathology sample be directly exposed in air) is kept in the incubation time of clock, according to the big of sample
Small difference, even if preventing dry plate measure, the primary antibody amount of reagent of most expensive using some above-mentioned automatic staining machines are adoptable
Will be between 100~200 microlitres, the reagent cost for completing one piece of pathology piece dyeing is not less than 100 yuan, and many users are caused
Larger cost pressure.
Simultaneously traditional slide is all made of the sheet glass using tabula rasa.
Existing anti-dry plate method shortcoming is at present：The service speed drawn a circle one by one with special wax crayon is slow, is not easily accomplished
Automation mechanized operation, influence checkability.If preventing dry plate using wet box, need that reagent, flushing, incubation process are added dropwise every time
Need to be put into taking-up operation repeatedly, it is difficult to realize automation；Then need to prepare a kind of extra finish using the anti-drying measure of oil dripping,
Oil film can usually cause punching not wash clean, influence last dyeing, film-making effect with easily being adhered to during sample incubation
Fruit.Prevent that dry plate shortcoming from being using cover glass neck method, because reagent, flushing liquor will be from slides and cover glass gap
Penetrate into, developing result is often not so good, influences Color.
Along with the development of medical technology, need to carry out histotomy dye in increasing disease academic research and diagnosis
Color, Color acts on more and more important, traditional tabula rasa slide and cover glass played in pathological examination can not meet
Present clinical and the demand of scientific research.
The content of the invention
In view of the shortcomings of the prior art, the technical problems to be solved by the invention are to propose a kind of convenient operation, save
Reagent, reduce cost, with special cover glass be used cooperatively can reduce reagent overflow, prevent tissue dry plate flange slide and
Prevent dry cover glass and wire frame preparation method.
The flange slide of above mentioned problem is can solve the problem that, its technical scheme includes rectangle lamellar body, except that the square
Shape lamellar body front is provided with single or two or more rectangle or circular or obround elements lattice are used to place histotomy progress
Dyeing, described variously-shaped cell are consistent and equal in the upper raised wire frame in lamellar body front, the height of projection of the wire frame
For 10um~1000um, the width of wire frame is between 0.1mm~10mm.
The height of projection of the wire frame is preferably 20um~400um, and width is preferably 0.5mm~5mm.
The height of projection of the wire frame most preferably 30um~300um, optimum width are 1mm~2mm, can accommodate sufficient amount
Incubation reagent, will not cause to waste again.
Single rectangle or circular or obround elements lattice in the middle part of rectangle lamellar body longitudinal direction or middle and lower part, horizontal centering or
Misalign setting.
Two rectangles or circular or obround elements lattice are set in longitudinal arrangement on rectangle lamellar body.
More than three rectangular unit grids are in horizontal, longitudinal direction or the setting that is staggered on rectangle lamellar body.
To prevent histotomy flake, anticreep coating is coated with the rectangle lamellar body front.
Can before rectangle or circular or obround elements lattice make applying coating, also can be in rectangle or circular or oval
Shape cell coats anticreep coating after making.
For ease of operating personnel's record or adhesive label, the termination of the rectangle lamellar body is left room as writing or attached
Flag.
For the anti-dry plate cover glass of flange slide, its area can be suitable with slide or raised with maximum on slide
The area of wire frame is suitable so that consistent closed in a gap is formed between cover glass and slide and the raised wire frame of covering
Cavity.
Hydrophilicity-imparting treatment can be carried out to the face that cover glass and slide are affixed, cover glass is covered in salient line frame on slide
In incubation liquid level on when can prevent the generation of bubble；The edge printing of the another side of cover glass, some are non-hydrophilic for compound, spot printing
Point or strain line or frame, the point, strain line, frame have 5um~300um height of projection, to stack before preventing use
Cover glass be mutually adhered.
The preparation method of wire frame in flange slide, hydrophobic glass ink can be used to pass through silk-screen printing, baking and curing
After formed；Also can be formed with the hydrophobic glue-film stickup for being coated with adhesive sticker or PUR being punched, can also be with curable
Glue carries out dispensing and formed, and according to glue curing condition used, passes through spontaneous curing, crosslinking curing, heat cure, photocuring, electricity
The modes such as beamlet solidification are formed, and dispensing scheme can be completed manually or using automatic dropping glue equipment.
Beneficial effects of the present invention：
1st, flange slide of the present invention is coordinated fixed different size of group by the slide from different flange patterns
Section is knitted, it is simple to operate.
2nd, the present invention has a variety of partitioned organizations, can position histotomy subregion on slide, can directly use,
System need not be drawn piecewise with special wax crayon.
3rd, the present invention has the various patterns of sizes, can coordinate different size of tissue samples, so can be obvious
Reagent is saved, minimum amount can be reduced to 10ul~40ul.
4th, the raised wire frame of the present invention has certain altitude, can be in droplet-like because reagent table face has certain tension force
Allow pathological section to be immersed in slide reagent inframe, be advantageous to the incubation of histotomy.
5th, when the present invention is with preventing that dry cover glass is used cooperatively, raised wire frame makes flange slide surface with preventing dry cover glass
Between formed a fixed interval (FI) airtight cavity.
6th, the present invention is suitable for manually contaminating piece and instrument dye piece, in manually dye piece, it is not necessary to after wax crayon enclosure again
Reagent is added dropwise, only suitable slide fixed tissue slices need to be selected according to the size of histotomy, be dewaxed, antigen is repaiied
The processes such as multiple, dye piece, it is simple to operate；During instrument dye piece, anti-dry cover glass is covered after reagent is added dropwise, can maximum journey
Degree reduces the dosage of reagent, while prevents dry plate phenomenon from occurring.
Brief description of the drawings
Fig. 1 is the three-dimensional structure diagram of one embodiment of the present invention.
Fig. 2 is A-A sectional views in Fig. 1.
Fig. 3 (a) is the first structure chart of Fig. 1, Fig. 2 flange slide.
Fig. 3 (b) is second of structure chart of Fig. 1, Fig. 2 flange slide.
Fig. 3 (c) is the third structure chart of Fig. 1, Fig. 2 flange slide.
Fig. 3 (d) is the 4th kind of structure chart of Fig. 1, Fig. 2 flange slide.
Fig. 3 (e) is the 5th kind of structure chart of Fig. 1, Fig. 2 flange slide.
Fig. 3 (f) is the 6th kind of structure chart of Fig. 1, Fig. 2 flange slide.
Fig. 3 (g) is the 7th kind of structure chart of Fig. 1, Fig. 2 flange slide.
Fig. 4 (a) is Fig. 3 (a) structure chart.
Fig. 4 (b) is Fig. 4 (a) side view.
Figure number identifies：1st, rectangle lamellar body；2nd, wire frame；3rd, flag；4th, dry cover glass is prevented.
Embodiment
Illustrated embodiment is described further to technical scheme below in conjunction with the accompanying drawings.
Flange slide of the present invention, for the rectangle lamellar body 1 with state's dimensioning, (length direction is longitudinal, width to its main body
Direction is laterally), making on the front of rectangle lamellar body 1 has the rows of rectangle of single or two or more or circular or ellipse
Cell (is dyed) for placing histotomy, and the rectangle or circular or obround elements lattice are on lamellar body front
Raised wire frame 2, the upper end of rectangle lamellar body 1 flow out flag 3 of the room as operating personnel's written record or adhesive label,
Coated with the anticreep coating for preventing that histotomy from departing from the surface of rectangle lamellar body 1, forward and backward coating anticreep can be made in cell and applied
Layer, the height of projection of the wire frame 2 is consistent and is 10um~1000um, width between 0.1mm~10mm, in fact it is highly preferred that
Preferred 0.5mm~the 5mm of 20um~400um, width, height most preferably 30um~300um, width most preferably 1mm~2mm, such as schemes
1st, Fig. 2, Fig. 4 (a), Fig. 4 (b) are shown.
In the case of using single rectangular unit grid, the rectangular unit grid longitudinally located at the middle and lower part of rectangle lamellar body 1 and
Horizontal centering, the top of the rectangle lamellar body 1 is flag 3, as shown in Fig. 3 (a).
In the case of using single rectangular unit grid, the rectangular unit grid is longitudinally located at the middle part of rectangle lamellar body 1 and horizontal stroke
To centering, the top of the rectangle lamellar body 1 is flag 3, as shown in Fig. 3 (b).
In the case of two rectangular unit grids, two rectangular unit grids are in the middle and lower part longitudinal arrangement of rectangle lamellar body 1
Setting and horizontal centering, the top of the rectangle lamellar body 1 is flag 3, as shown in Fig. 3 (c).
In the case of three rectangular unit grids, three rectangular unit grids on the middle and lower part of rectangle lamellar body 1 in longitudinally arranging
Row are set and horizontal centering, and the top of the rectangle lamellar body 1 is flag 3, as shown in Fig. 3 (d).
In the case of using single rectangular unit grid, the rectangular unit grid is longitudinally located at the middle part of rectangle lamellar body 1 and horizontal stroke
To centering, the bottom blank space of rectangle lamellar body 1 adds auxiliary frame to realize the balance of cover glass, the top of the rectangle lamellar body 1
For flag 3, as shown in Fig. 3 (e).
In the case of using single circular cell lattice, the circular cell lattice are located at the middle part or middle and lower part of rectangle lamellar body 1
And horizontal centering, the top of the rectangle lamellar body 1 is flag 3, as shown in Fig. 3 (f).
In the case of two circular cell lattice, two circular cell lattice on the middle and lower part of rectangle lamellar body 1 in longitudinally arranging
Row are set and horizontal centering, and the top of the rectangle lamellar body 1 is flag 3, as shown in Fig. 3 (g).
One of skill in the art can make certain adjustment to pattern magnitude, position, shape on the basis of the present invention,
Protection scope of the present invention is not departed from.
The present invention prevents that dry cover glass 4 is used cooperatively with slide, and its size can be with maximum raised wire frame 2 on slide
The cell area impaled is suitable or slightly larger so that prevents being formed between dry cover glass 4 and slide and the raised wire frame 2 of covering
The consistent airtight cavity in one gap, prevent that on the front that dry cover glass 4 is affixed with slide hydrophilicity-imparting treatment, Fang Gangai can be carried out
The edge printing of the another side of slide 4, the point or strain line or frame of some compound non-hydrophilics, the point, strain line, frame tool
There is 5um~300um height of projection, as shown in Figure 1 and Figure 2.
Prevent that the diascope or transparent plastic sheet or stainless steel substrates of 100um~1000um thickness can be selected in dry cover glass 4
Make, the diascope of preferably 170um~700um thickness is made.
Flanged type slide wire frame preparation method of the present invention：Hydrophobic glass ink can be used to pass through silk-screen printing, drying
Wire frame is formed after solidification, it is also possible to which what is be punched is coated with the hydrophobic glue-film stickup formation wire frame of PUR or adhesive sticker, may be used also
Formed with curable glue (spontaneous curing, heat cure, photocuring, electronic beam curing) spot printing.
Claims (13)
1. flange slide, including rectangle lamellar body (1), it is characterised in that：Rectangle lamellar body (1) front is provided with single or two
Individual above rectangle or circular or obround elements lattice are dyed for placing histotomy, described variously-shaped cell
For in wire frame (2) raised on rectangle lamellar body (1) front, the height of projection of the wire frame (2) it is consistent and be 10um~
1000um, the width of wire frame (2) is between 0.1mm~10mm.
2. flange slide according to claim 1, it is characterised in that：The height of projection of the wire frame (2) be 20um~
400um, width are 0.5mm~5mm.
3. flange slide according to claim 2, it is characterised in that：The height of projection of the wire frame (2) be 30um~
300um, optimum width are 1mm~2mm.
4. the flange slide according to any one in claims 1 to 3, it is characterised in that：Single rectangle or circle,
Or obround elements lattice in the middle part of rectangle lamellar body longitudinal direction or middle and lower part, horizontal centering or misalign setting.
5. the flange slide according to any one in claims 1 to 3, it is characterised in that：Two rectangles or circle,
Or obround elements lattice are set in longitudinal arrangement on rectangle lamellar body (1).
6. the flange slide according to any one in claims 1 to 3, it is characterised in that：More than three rectangles or circle
Shape or obround elements lattice are in horizontal, longitudinal direction or the setting that is staggered on rectangle lamellar body (1).
7. the flange slide according to any one in claims 1 to 3, it is characterised in that：The rectangle lamellar body (1) is just
Coated with the anticreep coating for preventing histotomy flake on face.
8. flange slide according to claim 7, it is characterised in that：Can be in rectangle or circular or obround elements lattice
Coating anticreep coating before or after making.
9. the flange slide according to any one in claims 1 to 3, it is characterised in that：The rectangle lamellar body (1)
Leave room as writing or attach flag (3) in termination.
10. the anti-dry cover glass (4) for the flange slide as described in any one in claims 1 to 3, it is characterised in that：
Its area can be suitable with slide or suitable with the outer area that maximum raised wire frame (2) is enclosed on slide so that cover glass with
The consistent airtight cavity in a gap is formed between slide and the raised wire frame (2) of covering.
11. according to claim 10 prevent dry cover glass (4), it is characterised in that：To preventing dry cover glass (4) and slide phase
The face progress hydrophilicity-imparting treatment of patch, edge printing, point compound, spot printing is non-hydrophilic or the strain line of anti-dry cover glass (4) another side,
Or frame, the point, strain line, frame have 5um~300um height of projection.
12. according to claim 10 prevent dry cover glass, it is characterised in that：Prevent dry cover glass (4) from 100um~
The diascope or transparent plastic sheet or stainless steel substrates of 1000um thickness make.
13. flange slide wire frame preparation method, it is characterised in that：It is solid by silk-screen printing, drying with hydrophobic glass ink
Formed after change；Or formed with the hydrophobic glue-film stickup for being coated with adhesive sticker or PUR being punched；Or with curable glue
It is formed by curing by craft or spot gluing equipment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711021345.5A CN107632384A (en) | 2017-10-26 | 2017-10-26 | Flange slide and anti-dry cover glass and wire frame preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711021345.5A CN107632384A (en) | 2017-10-26 | 2017-10-26 | Flange slide and anti-dry cover glass and wire frame preparation method |
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| Publication Number | Publication Date |
|---|---|
| CN107632384A true CN107632384A (en) | 2018-01-26 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711021345.5A Withdrawn CN107632384A (en) | 2017-10-26 | 2017-10-26 | Flange slide and anti-dry cover glass and wire frame preparation method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110285999A (en) * | 2019-07-08 | 2019-09-27 | 肯维捷斯（武汉）科技有限公司 | A kind of solidliquid mixture sampler and its sampling method |
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| CN101314755A (en) * | 2007-05-30 | 2008-12-03 | 中山大学达安基因股份有限公司 | Space-sharing hybridization reaction device for biological chip |
| CN102206573A (en) * | 2011-03-22 | 2011-10-05 | 博奥生物有限公司 | Automatic sample introduction device for microarray chip and automatic sample introduction hybridization microarray chip |
| CN204848871U (en) * | 2015-06-09 | 2015-12-09 | 上海北昂精密光电仪器股份有限公司 | Sperm count pond |
| CN206208629U (en) * | 2016-11-25 | 2017-05-31 | 上海美吉医学检验有限公司 | A kind of immunofluorescent staining slide |
| CN207336920U (en) * | 2017-10-26 | 2018-05-08 | 桂林优利特医疗电子有限公司 | Flange glass slide and anti-dry coverslip |
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2017
- 2017-10-26 CN CN201711021345.5A patent/CN107632384A/en not_active Withdrawn
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101314755A (en) * | 2007-05-30 | 2008-12-03 | 中山大学达安基因股份有限公司 | Space-sharing hybridization reaction device for biological chip |
| CN102206573A (en) * | 2011-03-22 | 2011-10-05 | 博奥生物有限公司 | Automatic sample introduction device for microarray chip and automatic sample introduction hybridization microarray chip |
| CN204848871U (en) * | 2015-06-09 | 2015-12-09 | 上海北昂精密光电仪器股份有限公司 | Sperm count pond |
| CN206208629U (en) * | 2016-11-25 | 2017-05-31 | 上海美吉医学检验有限公司 | A kind of immunofluorescent staining slide |
| CN207336920U (en) * | 2017-10-26 | 2018-05-08 | 桂林优利特医疗电子有限公司 | Flange glass slide and anti-dry coverslip |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110285999A (en) * | 2019-07-08 | 2019-09-27 | 肯维捷斯（武汉）科技有限公司 | A kind of solidliquid mixture sampler and its sampling method |
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Application publication date: 20180126 |