CN101288380A - Good quality, high yield, disease-resistant and wide adaptation ultra-sweet corn breeding method - Google Patents
Good quality, high yield, disease-resistant and wide adaptation ultra-sweet corn breeding method Download PDFInfo
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- CN101288380A CN101288380A CNA2008100268734A CN200810026873A CN101288380A CN 101288380 A CN101288380 A CN 101288380A CN A2008100268734 A CNA2008100268734 A CN A2008100268734A CN 200810026873 A CN200810026873 A CN 200810026873A CN 101288380 A CN101288380 A CN 101288380A
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Abstract
The invention pertains to a crop breeding field, and discloses a breeding method of super sweet corn with good quality, high yield, disease resistance and wide adaptability. the method adopts polycross method and integrates the good properties of tropical and temperate sweet corns and common corn to breed a male parent inbred line with high yield, disease resistance, wide adaptability, good quality and strong association; the method also adopts pedigree selection method to breed an excellent female parent inbred line so as to breed the super sweet corn with good quality, high yield, disease resistance and wide adaptability. The corn has the advantages of thin peel, high content of sugar, high yield, disease resistance and wide adaptability, etc.
Description
Technical field
The invention belongs to the crop breeding field, particularly a kind of selection of cultivating high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn.
Background technology
The inbred line of sweet corn selection generally has three kinds: one is the selection cross method: adopt inbred line of sweet corn and conventional corn hybridization between selfed lines, select inbred line of sweet corn then from filial generation; Two are the backcross breeding method: with the inbred line of sweet corn with objective trait is non-recurrent parent, and the conventional corn selfing is a recurrent parent, adopts and backcrosses the transformation of sweet corn objective trait in conventional corn, selects inbred line of sweet corn.Three is that directly choosing is a method: select inbred line of sweet corn by pedigree method from sweet corn landrace and single cross hybrid.The inbred line that selects with above-mentioned three kinds of methods is hybridized combo and is bred the sweet corn kind.Selection cross method and backcross breeding method are significantly to the resistance of improving sweet corn and output effect, but the sweet corn kind of breeding the thus wide and inferior quality of adaptability often.Though directly choosing is that method is easier to guarantee the inbred line of sweet corn quality,, therefore be difficult to select good inbred line of comprehensive proterties or kind because most of sweet corn landrace heredity genetic background is approaching and resistance is low, bad adaptability.
Summary of the invention
In order to solve the but deficiency of strong, the bad adaptability of high-quality or high-quality but low yield, resistance not of inbred line that above-mentioned inbred line breeding method selects or high yield, resistance, the object of the present invention is to provide the breeding method of a kind of high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn, this method is improved the selection of inbred line of sweet corn, selects the good sweet corn of comprehensive proterties.
Purpose of the present invention is achieved through the following technical solutions: the breeding method of a kind of high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn comprises the steps:
(1) adopts polycross method seed selection male parent inbred line
Hot belt type sweet corn and temperate zone type sweet corn are hybridized, after recurrent selection, select the inbred line of wide adaptability and high-quality, then with this inbred line and conventional corn hybridization between selfed lines, use the pedigree selection-breeding method, from its offspring, select high yield, disease-resistant, wide suitable, the more excellent inbred line of quality is made male parent.
(2) cultivate the maternal inbred line of high-quality by the pedigree back-and-forth method
From the high-quality sweet corn,, adopt the pedigree back-and-forth method to select sugariness height, mouthfeel is good, skin is thin maternal inbred line through inbreeding of more generation.
(3) with above-mentioned two inbred lines preparation combination, cultivate high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn kind.
The breeding method of above-mentioned high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn specifically comprises the steps:
(1) adopts polycross method seed selection male parent inbred line: the hot belt type sweet corn is done maternal hybridization with temperate zone type sweet corn to obtain F1 for seed; The adaptability that begins to carry out plant from F2 generation is selected, selecting the inbred line of wide adaptability and high-quality after 10 generations were selected, is maternal with this selfing then, and conventional corn ' yellow gingival cyst of mucous gland in the newborn ' is hybridized for male parent, use the pedigree selection-breeding method, from its offspring, select the male parent inbred line.
(2) cultivate the maternal inbred line of high-quality by the pedigree back-and-forth method: with the high-quality sweet corn serves as to select colony, adopts the pedigree back-and-forth method, obtains sugariness height, mouthfeel is good, skin is thin maternal inbred line through the selfing of 6 generations; The selection criteria in described pedigree back-and-forth method per generation is for the grain look yellowish, sugariness is high, skin is thin, fringe does not have bald.
(3) with male parent inbred line and maternal inbred line two inbred lines preparation combination, cultivate high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn.
In order to realize the present invention better, hot belt type sweet corn described in the step (1) is Thailand hot belt type sweet corn SC011, and described temperate zone type sweet corn is U.S. temperate zone type sweet corn SC056.
Plant selfing or hybridization that the selection of adaptability described in the step (1) promptly is chosen in more than 32 ℃ or can normally blossoms and has seeds below 17 ℃, and select the maximum single bud of solid number to reserve seed for planting.
The high-quality sweet corn is U.S. high-quality sweet corn POP4 in the step (2).
When the adaptability that begins to carry out plant from F2 generation in the step (1) was selected, the stubble sowing time in spring of male parent seed selection was May 15, on October 15 stubble sowing time in autumn, selected more than 32 ℃ respectively or the plant of 17 ℃ of pollinations of normally blooming down carry out selfing or hybridization.Select to be higher than 32 ℃ preferred especially spring, and the plant of selecting to be lower than 17 ℃ autumn carries out selfing or hybridization.
The present invention is by polycross, and male parent has been integrated the sweet corn gene and the conventional corn gene of hot belt type, temperate zone type, and is maternal then the high-quality gene of U.S.'s sweet corn is arranged, so first generation of hybrid sweet corn has high-quality, high yield, disease-resistant, wide merit such as suitable.
The present invention compared with prior art, have following outstanding advantage and beneficial effect: the inventive method adopts polycross method, integrate hot belt type, temperate zone type sweet corn and conventional corn merit, cultivated high yield, disease-resistant, wide suitable, high-quality and the high male parent inbred line of coordinate force; Cultivate the maternal inbred line of high-quality by the pedigree back-and-forth method, select high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn.Advantages such as this super-sweet corn kind has that pericarp is thin, sugar content is high, high yield, disease-resistant, wide adaptability.
Description of drawings
Fig. 1 is a male parent seed selection schematic flow sheet.
Fig. 2 is maternal seed selection schematic flow sheet.
Embodiment
The present invention is described in further detail below in conjunction with embodiment and accompanying drawing, but embodiments of the present invention are not limited thereto.
Embodiment 1
1, the seed selection of high yield, disease-resistant, wide suitable, male parent inbred line ' 013 ' of fine quality, the seed selection of male parent sees Fig. 1 for details.
In order to select adaptable male parent inbred line, be May 15 from F2 for the beginning stubble sowing time in spring, the plant that pollinates of selecting can normally bloom more than 32 ℃ carries out selfing or hybridization; On October 15 stubble sowing time in autumn, select the plant selfing or the hybridization of 17 ℃ of pollinations of normally blooming down.
(1) first year spring done maternal hybridization with U.S. temperate zone sweet corn resource SC056 to obtain the F1 seed with Thailand hot belt type sweet corn resource SC011.Plant filial generation 20 strains autumn, select 3 strain robust growth, sweet, the fringe of flavor not to have bald sharp individual plant selfing, obtain F2 for seed.
F2 generation 723 strains are planted in spring sowing in (2) second years.Select 10 many strains of pollen amount to carry out pollination self, and select 6 maximum in single bud of solid number to reserve seed for planting acquisition F3 generation.In planted in fall F3 generation,, select normally to bloom loose powder and colory not homophyletic are that plant hybridizes mutually, obtain F4 generation totally 4 strains systems.
40 strains were planted by the every strain of spring and autumn system in (3) the 3rd years, and continuing to select the many not homophyletics of pollen amount is good plant hybridization, and the selection fringe does not have bald point and 1 high in single bud of seed-producing rate is reserved seed for planting, and obtains F6 generation 2 strain systems then autumn.
Selected strain system carried out continuous selfing in (4) the 4th years~the 5th year, per generation selection criteria the same, screening obtains the inbred line of high-quality, wide adaptability, and ' Guangdong section is from 99 '.
(5) the 6th year spring is so that ' Guangdong section is from 99 ' being female parent, and conventional corn ' yellow gingival cyst of mucous gland in the newborn ' is a paternal hybrid, obtains F1 for crossbreed.20 strains and the selfing of pollinating are planted in autumn planting in the 6th year, select 3 single buds to mix to reserve seed for planting after roguing and obtain F2 for crossbreed.
Spring sowing in (6) the 7th years is planted F2 for crossbreed 50 strains, selects behind the pollination self that bud is thick, fringe do not have 5 bald single buds and reserve seed for planting and obtain F3 generation 5 strains systems.Plantation 40 strains of every strain of the 7th year autumn system.Select respectively to select 10 strains to carry out selfing from 3 strain systems according to the growth performance, every strain is to select one in sugariness height, no bald single bud, obtains F4 for 3 strain systems.
Kind of F4 sowed in spring in (7) the 8th years for crossbreed, and so far existing 2 strains are to show basically identical between offspring's individuality, select output height, disease-resistant 1 strain system strong, that product are of fine quality to mix to collect seed and obtain male parent inbred line ' 013 '.
2, the cultivation of the maternal inbred line ' 096 ' of high-quality, maternal seed selection sees Fig. 2 for details.
In (1) first year spring, plantation U.S. high-quality sweet corn resource POP420 strain is selected fringe not have 1 bald single bud and is reserved seed for planting acquisition F2 generation behind the pollination self.In 1 year autumn, plantation F2 generation 50 strains select behind the pollination self that the grain look yellowish, sugariness is high, skin is thin, fringe do not have 3 bald single buds and collect seed, acquisition F3 generation.
Spring sowing in (2) second years is planted F3 generation, and every strain is 50 strains and carries out pollination self, continues to select that the grain look yellowish, sugariness is high, skin is thin, fringe do not have bald single bud and collect seed, and obtains 4 strains of F4 generation to be.Plantation 50 strains of every strain of 1 year autumn system.Select 2 strains system to carry out pollination self according to qualifications according to the progeny population general performance, select that the grain look yellowish, sugariness is high, skin is thin equally, fringe do not have bald single bud and collect seed, acquisition F5 generation.
Sowed in spring in (3) the 3rd years and plant F5 generation.This F5 generation existing 3 strains system individual performance basically identical is selected the fringe straight barrel type, and leaf is bud green, and 1 strain that boot leaf is arranged is to mix behind the pollination self to collect seed, and obtains inbred line ' 096 '.
3, the seed selection of high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn
Make male parent with inbred line ' 013 ', do maternal assembly, through kind relatively, select high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn with the inbred line ' 096 ' of seed high-quality.
4, high-quality of the present invention, high yield, disease-resistant, wide adaptation ultra-sweet corn characteristic:
The plant type hemicompact is gathered, and the plant growing situation is energetic, about 185~195 centimetres of plant heights, about 62~67 centimetres of fringe positions; about 20 centimetres of spike length, thick about 4.6 centimetres of fringe, about single fringe fresh weight 280 grams, the seed yellow; soluble sugar content 18.37%, pericarp is thinner, and palatability is better, and product are of fine quality.The long cartridge type of fruit ear, tassel row number 14~16 row, marshalling, wide adaptability, more anti-banded sclerotial blight, the Chinese People's Anti-Japanese Military and Political College, helminthosporium maydis, resistant to lodging.To results, sowed in spring about 85 days in Guangdong from sowing, and autumn sowing is about 75 days, about 1000 kilograms of every per mu yield.
Compare with the main breed ' sweet No. 1 of fringe ' on producing, high-quality of the present invention, high yield, advantage disease-resistant, wide adaptation ultra-sweet corn are:
(1) high-quality: contrast ' sweet No. 1 of fringe ' soluble sugar content is 17.2%, thick 3.2 grades of pericarp (tasting 1~4 grade of rank, down together).The soluble sugar content 18.37% of high-quality of the present invention, high yield, disease-resistant, wide adaptation ultra-sweet corn, thick 2.9 grades of pericarp, this kind has obtained breakthrough aspect peel thickness.
(2) high yield: high-quality of the present invention, high yield, disease-resistant, wide adaptation ultra-sweet corn average yield per mu 865.69kg, ' sweet No. 1 of fringe ' average yield per mu is 771.49kg, than ' sweet No. 1 of fringe ' volume increase 12.05%.
(3) disease-resistant: high-quality of the present invention, high yield, disease-resistant, wide adaptation ultra-sweet corn to banded sclerotial blight, northern and southern leaf blight reach respectively anti-, in anti-level, its resistance level to the main disease of sweet corn meets or exceeds ' sweet No. 1 of fringe '.
(4) wide suitable: wide adaptability, can be in the torrid zone, subtropics etc. the zone cultivate.
(5) drought-enduring: the drought resistance result of the test that carry out autumn Guangdong Province academy of agricultural sciences crop institute corn chamber in 2006 shows, high-quality of the present invention, high yield, disease-resistant, the per mu yield of wide adaptation ultra-sweet corn under drought condition are 723.4kg, ' sweet No. 3 of Guangdong ' (national sweet corn district examination check variety) per mu yield 631.4kg, comparison reaches more than the significance level according to kind of a volume increase 14.5%.High-quality of the present invention, high yield, disease-resistant, wide adaptation ultra-sweet corn under drought stress under main proterties and the normal moisture condition difference not obvious, prove absolutely that this kind drought resistance is strong, high yield, stable yields.
(6) the easily production of hybrid seeds: male parent has been integrated the merit of the torrid zone, temperate zone and domestic conventional corn, and disease resistance is strong, wide adaptability; Therefore woody ripening rate height has improved the hybrid seed yield of high-quality of the present invention, high yield, disease-resistant, wide adaptation ultra-sweet corn.
The foregoing description is a preferred implementation of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spiritual essence of the present invention and the principle, substitutes, combination, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (5)
1, the breeding method of a kind of high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn is characterized in that comprising the steps:
(1) adopts polycross method seed selection male parent inbred line: the hot belt type sweet corn is done maternal hybridization with temperate zone type sweet corn to obtain F1 for seed; The adaptability that begins to carry out plant from F2 generation is selected, selecting the inbred line of wide adaptability and high-quality after 10 generations were selected, is maternal with this selfing then, and conventional corn ' yellow gingival cyst of mucous gland in the newborn ' is hybridized for male parent, use the pedigree selection-breeding method, from its offspring, select the male parent inbred line;
(2) cultivate the maternal inbred line of high-quality by the pedigree back-and-forth method: with the high-quality sweet corn serves as to select colony, adopts the pedigree back-and-forth method, obtains sugariness height, mouthfeel is good, skin is thin maternal inbred line through the selfing of 6 generations; The selection criteria in described pedigree back-and-forth method per generation is for the grain look yellowish, sugariness is high, skin is thin, fringe does not have bald;
(3) with male parent inbred line and maternal inbred line two inbred lines preparation combination, cultivate high-quality, high yield, disease-resistant, wide adaptation ultra-sweet corn.
2, the breeding method of a kind of high-quality according to claim 1, high yield, disease-resistant, wide adaptation ultra-sweet corn, it is characterized in that: hot belt type sweet corn described in the step (1) is Thailand hot belt type sweet corn SC011, and described temperate zone type sweet corn is U.S. temperate zone type sweet corn SC056.
3, the breeding method of a kind of high-quality according to claim 1, high yield, disease-resistant, wide adaptation ultra-sweet corn, it is characterized in that: plant selfing or hybridization that the selection of adaptability described in the step (1) promptly is chosen in more than 32 ℃ or can normally blossoms and has seeds below 17 ℃, and select the maximum single bud of solid number to reserve seed for planting.
4, the breeding method of a kind of high-quality according to claim 1, high yield, disease-resistant, wide adaptation ultra-sweet corn, it is characterized in that: the high-quality sweet corn is U.S. high-quality sweet corn POP4 in the step (2).
5, the breeding method of a kind of high-quality according to claim 1, high yield, disease-resistant, wide adaptation ultra-sweet corn, it is characterized in that: when the adaptability that begins to carry out plant from F2 generation in the step (1) is selected, the stubble sowing time in spring of male parent seed selection is May 15, on October 15 stubble sowing time in autumn, select to be higher than 32 ℃ or select to be lower than 17 ℃ plant autumn and carry out selfing or hybridization spring.
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Cited By (11)
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CN103026963A (en) * | 2012-12-28 | 2013-04-10 | 四川雅玉科技开发有限公司 | Breeding method for temperate corn employing tropical germplasm |
CN105850712A (en) * | 2016-04-05 | 2016-08-17 | 广东省农业科学院作物研究所 | Breeding method of strong-superiority hybrid seeds of super-sweet corn |
CN106212266A (en) * | 2016-07-30 | 2016-12-14 | 南京市蔬菜科学研究所 | A kind of selection of the double-colored super-sweet corn of HUANGBAI(sic) |
CN106472302A (en) * | 2016-10-28 | 2017-03-08 | 福建农林大学 | A kind of selection of extensively anti-Sweet corn variety |
CN106508664A (en) * | 2016-10-28 | 2017-03-22 | 福建农林大学 | Breeding method of high-stress-resistance sweet corn variety |
CN106718831A (en) * | 2016-11-30 | 2017-05-31 | 广西青青农业科技有限公司 | A kind of strong advantage warms the selection of corn hybrid seed |
CN107372100A (en) * | 2017-08-23 | 2017-11-24 | 四川省农业科学院水稻高粱研究所 | A kind of method of Sorghum Hybrid breeding |
CN107787830A (en) * | 2016-08-31 | 2018-03-13 | 辽宁东亚农业发展有限公司 | A kind of method for improving Temperate maize variety and quality and resistance |
CN109287476A (en) * | 2018-08-23 | 2019-02-01 | 温州科技职业学院 | A kind of screening technique of the compatible corn new varieties of high yield and high quality |
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CN111670799A (en) * | 2020-06-16 | 2020-09-18 | 温州科技职业学院 | Sweet corn breeding method |
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CN103026963A (en) * | 2012-12-28 | 2013-04-10 | 四川雅玉科技开发有限公司 | Breeding method for temperate corn employing tropical germplasm |
CN105850712A (en) * | 2016-04-05 | 2016-08-17 | 广东省农业科学院作物研究所 | Breeding method of strong-superiority hybrid seeds of super-sweet corn |
CN106212266A (en) * | 2016-07-30 | 2016-12-14 | 南京市蔬菜科学研究所 | A kind of selection of the double-colored super-sweet corn of HUANGBAI(sic) |
CN106212266B (en) * | 2016-07-30 | 2018-10-19 | 南京市蔬菜科学研究所 | A kind of selection of yellowish-white double-colored super-sweet corn |
CN107787830A (en) * | 2016-08-31 | 2018-03-13 | 辽宁东亚农业发展有限公司 | A kind of method for improving Temperate maize variety and quality and resistance |
CN106472302A (en) * | 2016-10-28 | 2017-03-08 | 福建农林大学 | A kind of selection of extensively anti-Sweet corn variety |
CN106508664A (en) * | 2016-10-28 | 2017-03-22 | 福建农林大学 | Breeding method of high-stress-resistance sweet corn variety |
CN106718831A (en) * | 2016-11-30 | 2017-05-31 | 广西青青农业科技有限公司 | A kind of strong advantage warms the selection of corn hybrid seed |
CN107372100A (en) * | 2017-08-23 | 2017-11-24 | 四川省农业科学院水稻高粱研究所 | A kind of method of Sorghum Hybrid breeding |
CN109287476A (en) * | 2018-08-23 | 2019-02-01 | 温州科技职业学院 | A kind of screening technique of the compatible corn new varieties of high yield and high quality |
CN110063224A (en) * | 2019-05-30 | 2019-07-30 | 湖南省作物研究所 | A kind of In Cadmium Polluted Area spring open country watermelon-autumn corn safety in production rotation system |
CN111670799A (en) * | 2020-06-16 | 2020-09-18 | 温州科技职业学院 | Sweet corn breeding method |
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