CN101248773B - Breeding method of Wubie parent and breeding method thereof - Google Patents
Breeding method of Wubie parent and breeding method thereof Download PDFInfo
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- CN101248773B CN101248773B CN2008100608191A CN200810060819A CN101248773B CN 101248773 B CN101248773 B CN 101248773B CN 2008100608191 A CN2008100608191 A CN 2008100608191A CN 200810060819 A CN200810060819 A CN 200810060819A CN 101248773 B CN101248773 B CN 101248773B
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- 238000009395 breeding Methods 0.000 title claims abstract description 15
- 241001482311 Trionychidae Species 0.000 claims abstract description 109
- 238000000034 method Methods 0.000 claims abstract description 14
- 230000001488 breeding effect Effects 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 19
- 239000004576 sand Substances 0.000 claims description 12
- 230000012447 hatching Effects 0.000 claims description 10
- 235000013601 eggs Nutrition 0.000 claims description 8
- 102000002322 Egg Proteins Human genes 0.000 claims description 7
- 108010000912 Egg Proteins Proteins 0.000 claims description 7
- 210000004681 ovum Anatomy 0.000 claims description 7
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 6
- 241001465754 Metazoa Species 0.000 claims description 5
- 230000037396 body weight Effects 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 230000002265 prevention Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 235000012255 calcium oxide Nutrition 0.000 claims description 3
- 239000000292 calcium oxide Substances 0.000 claims description 3
- 241000411851 herbal medicine Species 0.000 claims description 3
- 238000009396 hybridization Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 3
- 239000012286 potassium permanganate Substances 0.000 claims description 3
- 230000002068 genetic effect Effects 0.000 abstract description 4
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 230000000050 nutritive effect Effects 0.000 abstract description 2
- 241000270666 Testudines Species 0.000 abstract 3
- 238000009402 cross-breeding Methods 0.000 abstract 1
- 235000019659 mouth feeling Nutrition 0.000 abstract 1
- 230000017448 oviposition Effects 0.000 abstract 1
- XLSLFPQAPYONPW-WHUHBCJBSA-N (2s,3s,4s,5r,6r)-6-[(r)-cyano(phenyl)methoxy]-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound O1[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O[C@@H](C#N)C1=CC=CC=C1 XLSLFPQAPYONPW-WHUHBCJBSA-N 0.000 description 4
- UNGXBWFICNLIRX-UHFFFAOYSA-N Laetrile Natural products NCC(OC1OC(C(O)C(O)C1O)C(=O)O)c2ccccc2 UNGXBWFICNLIRX-UHFFFAOYSA-N 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 230000004888 barrier function Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000006266 hibernation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 102000009027 Albumins Human genes 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000007926 Craterellus fallax Nutrition 0.000 description 1
- 102000004420 Creatine Kinase Human genes 0.000 description 1
- 108010042126 Creatine kinase Proteins 0.000 description 1
- 240000007175 Datura inoxia Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 206010017472 Fumbling Diseases 0.000 description 1
- 108020004206 Gamma-glutamyltransferase Proteins 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- 241000442132 Lactarius lactarius Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
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- 235000012000 cholesterol Nutrition 0.000 description 1
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- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 102000006640 gamma-Glutamyltransferase Human genes 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 229910052712 strontium Inorganic materials 0.000 description 1
- CIOAGBVUUVVLOB-UHFFFAOYSA-N strontium atom Chemical compound [Sr] CIOAGBVUUVVLOB-UHFFFAOYSA-N 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to the field of the aquiculture, in particular to methods of the breed selection of the soft-shelled turtle breed-parent and the breeding of the soft-shelled turtle. The breed selection of the soft-shelled turtle includes obtaining F1 by crossbreeding of the soft-shelled turtle individual and the strip-necked turtle; obtaining the soft-shelled turtle breed-parent F4 after breed selection of three generations, the genetic character thereof is stable at above 99%. The invention also discloses the breeding of the soft-shelled turtle, which includes introducing and breeding F4 as the breed-parent; egg-laying, egg taking, incubating, temporarily breeding of young turtles, breeding of baby turtles, stocking in the outer pond and obtaining the soft-shelled turtle. The bred soft-shelled turtle is characterized in good taste, good mouth feeling and high nutritive value.
Description
Technical field
The present invention relates to aquaculture field, relate in particular to black soft-shelled turtle parent's selection and the cultivation cultural method of black soft-shelled turtle.
Background technology
The crow soft-shelled turtle originates in Taihu Lake basin, and the locality claims " watt soft-shelled turtle " again.Its whole body and even belly all are corvinus, and the bodily form is similar to common soft-shelled turtle, also seldom see one in several thousand wild soft-shelled turtles.Interrelated data shows that Tokyo Univ Japan and rock paddy industry company once crossed research and confirm: " soft-shelled turtle has antitumaous effect ".Because contain laetrile in the soft-shelled turtle body, particularly the content in the shirt rim is than horn of plenty, and laetrile is the cancer-resisting substance of the United Nations's health organization approval.In the animal body that has melanin to exist, laetrile content is higher relatively according to inventor's research, so the laetrile content of clean brook crow soft-shelled turtle wants high than the common soft-shelled turtle on the market.
Clean brook, Zhejiang Province soft-shelled turtle industry Co., Ltd is in the stage of fumbling after trying to support black soft-shelled turtle since 1992 always, mainly is that the genetic character of black soft-shelled turtle is stable inadequately.Therefore, the extensive cultivation of black soft-shelled turtle is unsolved technical barrier always.
Summary of the invention
Cultivate the technical barrier of existence on a large scale in order to solve above-mentioned black soft-shelled turtle.An object of the present invention is to provide the black soft-shelled turtle parent's of stabilization characteristics of genetics selection.Another object of the present invention provides the cultivation cultural method of black soft-shelled turtle.
For first purpose of practising above-mentioned, the present invention has adopted following technical scheme:
Crow soft-shelled turtle parent's selection comprises the steps: 1. to select the individual and flower soft-shelled turtle hybridization of the black soft-shelled turtle with the pitch-black proterties of whole body, produces F
12. at F
1In select the entirely black black soft-shelled turtle individuality of proterties and carry out selfing, produce F
23. at F
2In select the entirely black individuality of proterties and carry out selfing again, produce F
34. at F
3In select the entirely black individuality of proterties and proceed selfing again, produce F
4Black soft-shelled turtle parent F through above-mentioned four generations seed selection
4Stabilization characteristics of genetics to 99% more than.
In order to realize second above-mentioned purpose, the present invention has adopted following technical scheme:
The cultivation cultural method of crow soft-shelled turtle, the F that adopts above-mentioned selection to obtain
4As parent culture, lay eggs, adopt that ovum, hatching, young soft-shelled turtle are supported temporarily, the soft-shelled turtle seedling is cultivated, the outer pool is put in a suitable place to breed and obtained described black soft-shelled turtle.As preferably, utilize F
4Parent's male and female ratio is 6: 1 when cultivating as the parent, and breeding density is 2m
2/ only.
As preferably, above-mentioned hatching step: select fertilized egg big, the body circle, the animal end is placed in the incubation plate up, incubation plate bottom is covered with the 3cm fine sand, and lid layer fine sand again after ovum is put well is sent into the hatching house then, hatch, hatching house's temperature remains on about 32 ℃, humidity 70%～75%.
As preferably, the young soft-shelled turtle that hatches must be supported 12～36 hours in the big basin of putting thick fine sand of 8～12cm and clear water temporarily, moved into young soft-shelled turtle again and cultivated in the pond.
As preferably, the soft-shelled turtle seedling is cultivated and is required young soft-shelled turtle to cultivate the pond to require into draining convenient, and the bottom is covered with the thick fine sand of 8～12cm; Be provided with feed table in the pond, by 80～120 young soft-shelled turtle/platform settings; The young soft-shelled turtle cultivation time is 4～8 months.
As preferably, before putting in a suitable place to breed, the outer pool of soft-shelled turtle seedling the soft-shelled turtle body is carried out disinfection with the potassium permanganate immersion of 20～30ppm 3～8 minutes.
As preferably, the outer pool time of putting in a suitable place to breed is 3 years, and breeding density is 1.5m
2/ only, bait feeding divides the upper and lower noon twice, adds Chinese herbal medicine in the bait and carries out diseases prevention, and the bait injected volume accounts for 3～5% of soft-shelled turtle body weight.Carry out water quality management work between the breeding period of the outer pool, when water transparence is lower than 20cm, in time more renew water, quantity of exchanged water is 1/5～1/2 of former Chi Shui.The outer pool is put Chi Shui in a suitable place to breed when pH value is lower than 7, and available quicklime water is splashed.
The present invention is because to have adopted above-mentioned technical scheme, pitch-black this genetic character of black soft-shelled turtle base plate to obtain stable, and the black soft-shelled turtle that institute cultivates breed has the advantages that taste perfume (or spice), mouthfeel are good, be of high nutritive value.
Embodiment
One, black soft-shelled turtle parent system seed selection
1. it is extremely rare to select occurring in nature, has the individual and flower soft-shelled turtle hybridization of black soft-shelled turtle of the pitch-black proterties of whole body, produces F
1
2. at F
1In select the entirely black black soft-shelled turtle individuality of proterties and carry out selfing, produce F
2
3. at F
2In select the entirely black individuality of proterties and carry out selfing again, produce F
3
4. at F
3In select the entirely black individuality of proterties and proceed selfing again, produce F
4Its genetic character of black soft-shelled turtle through the seed selection of four generations is stabilized to more than 99%.
Two, parent culture
Utilize F
4Cultivate as the parent, parent's male and female ratio is 6: 1, and breeding density is 2m
2/ only, fresh and alive animal bait accounts for 60% in the material of throwing something and feeding, and accounts for 40% based on the bait of import white fish meal, and interpolation diseases prevention traditional Chinese medicine powder was thrown something and fed 10 days in every month continuously in the bait, and the bait injected volume is 3～5% of a soft-shelled turtle body weight.
Three, lay eggs, adopt ovum
When spring, water temperature rose to more than 27 ℃, black soft-shelled turtle began to lay eggs, and can adopt ovum after laying eggs 24 hours.
Four, hatching
Select fertilized egg big, the body circle, the animal end is placed in the incubation plate up, and the incubation plate bottom is covered with the 3cm fine sand, and lid layer fine sand again after ovum is put well is sent into the hatching house then, hatches.Hatching house's temperature remains on about 32 ℃, and humidity 70%～75% is hatched 45 days young soft-shelled turtles in the left and right sides and begun shell.
Five, young soft-shelled turtle is supported temporarily
The young soft-shelled turtle that hatches must be supported 24 hours in the big basin that is placed with thick fine sand of 10cm and clear water temporarily, moved into young soft-shelled turtle again and cultivated in the pond.
Six, the soft-shelled turtle seedling is cultivated
Young soft-shelled turtle is cultivated the pond and requires into draining convenient, and the bottom is covered with the thick fine sand of 10cm, with convenient young soft-shelled turtle hibernation.Be provided with feed table in the pond, by 100 young soft-shelled turtle/platform settings.The young soft-shelled turtle cultivation time is about half a year, and put in a suitable place to breed on the pool outside can moving into after hibernation in April next year is revived, and with the potassium permanganate of 20-30ppm immersion 5 minutes the soft-shelled turtle body is carried out disinfection before young soft-shelled turtle is put in a suitable place to breed.
Seven, put in a suitable place to breed on the outer pool
The outer pool time of putting in a suitable place to breed is 3 years, and breeding density is 1.5m
2/ only.Bait feeding divides the upper and lower noon twice, adds Chinese herbal medicine in the bait and carries out diseases prevention, and vegetative period, absolute prohibition used any chemicalses such as antibiotic, hormone, no any medicament residue in the soft-shelled turtle body.The bait injected volume accounts for 3～5% of soft-shelled turtle body weight, during carry out water quality management work, when water transparence is lower than 20cm, in time more renew water, quantity of exchanged water is 1/5～1/2 of former Chi Shui, when pH value was lower than 7, available quicklime water was splashed.
Eight, the soft-shelled turtle pool is risen and is caught, sterilizes
After putting in a suitable place to breed through 3 years ecology, treat that soft-shelled turtle is long when above, can rise and catch list marketing to 0.5kg, and to the sterilization of cleaning up the pond of the soft-shelled turtle pool.By detection to black soft-shelled turtle and common soft-shelled turtle physical and chemical index and serum, find that the nutrition of black soft-shelled turtle is very obvious, see the following form:
| Title | Strontium (mg/kg) | Calcium (mg/kg) | Zinc (mg/kg) | Iron (mg/kg) | Creatine kinase (IU/L) | Lactate dehydrogenase (IU/L) | Glutamyl transpeptidase (IU/L) | Albumin (g/L) | Cholesterol (g/L) |
| The crow soft-shelled turtle | 4.5 | 2600 | 22 | 22.3 | 5328.43 | 8617.39 | 12.67 | 0.83 | 2.84 |
| Common soft-shelled turtle | 0.62 | 245 | 16.3 | 18.2 | 2378.13 | 6480.2 | 9.75 | 0.69 | 3.61 |
| Comparing result | The crow soft-shelled turtle exceeds 7 times | The crow soft-shelled turtle is high more than 10 times | The crow soft-shelled turtle is high more than 30% | The crow soft-shelled turtle is high more than 20% | The crow soft-shelled turtle is high more than 2 times | The crow soft-shelled turtle is high more than 30% | The crow soft-shelled turtle is high by about 30% | The crow soft-shelled turtle is high more than 20% | The crow soft-shelled turtle is low more than 25% |
Claims (10)
1. black soft-shelled turtle parent's selection is characterized in that comprising the steps: 1. to select the individual and flower soft-shelled turtle hybridization of the black soft-shelled turtle with the pitch-black proterties of whole body, produces F
12. at F
1In select the entirely black black soft-shelled turtle individuality of proterties and carry out selfing, produce F
23. at F
2In select the entirely black individuality of proterties and carry out selfing again, produce F
34. at F
3In select the entirely black individuality of proterties and proceed selfing again, produce F
4
2. the cultivation cultural method of black soft-shelled turtle is characterized in that: the F that adopts selection as claimed in claim 1 to obtain
4As parent culture, lay eggs, adopt that ovum, hatching, young soft-shelled turtle are supported temporarily, the soft-shelled turtle seedling is cultivated, the outer pool is put in a suitable place to breed and obtained described black soft-shelled turtle.
3. the cultivation cultural method of black soft-shelled turtle according to claim 2, it is characterized in that: parent's male and female ratio is 6: 1 when utilizing F4 to cultivate as the parent, and breeding density is 2m
2/ only.
4. the cultivation cultural method of black soft-shelled turtle according to claim 2, it is characterized in that: select fertilized egg big, the body circle, the animal end is placed in the incubation plate up, the incubation plate bottom is covered with the thick fine sand of 3cm, lid layer fine sand again after ovum is put well is sent into the hatching house then, hatches, hatching house's temperature remains on about 32 ℃, humidity 70%～75%.
5. the cultivation cultural method of black soft-shelled turtle according to claim 2, it is characterized in that: the young soft-shelled turtle that hatches must be supported 12～36 hours in the big basin of putting thick fine sand of 8～12cm and clear water temporarily, moved into young soft-shelled turtle again and cultivated in the pond.
6. the cultivation cultural method of black soft-shelled turtle according to claim 2 is characterized in that: the soft-shelled turtle seedling cultivate require young soft-shelled turtle to cultivate the pond to advance draining convenient, the bottom is covered with the thick fine sand of 8～12cm; Be provided with feed table in the pond, by 80～120 young soft-shelled turtle/platform settings; The soft-shelled turtle seedling cultivation time is 4～8 months.
7. the cultivation cultural method of black soft-shelled turtle according to claim 2 is characterized in that: with the potassium permanganate immersion of 20～30ppm 3～8 minutes the soft-shelled turtle body is carried out disinfection before put in a suitable place to breed on the outer pool of soft-shelled turtle seedling.
8. the cultivation cultural method of black soft-shelled turtle according to claim 2, it is characterized in that: the outer pool time of putting in a suitable place to breed is 3 years, breeding density is 1.5m
2/ only, bait feeding divides the upper and lower noon twice, adds Chinese herbal medicine in the bait and carries out diseases prevention, and the bait injected volume accounts for 3～5% of soft-shelled turtle body weight.
9. the cultivation cultural method of black soft-shelled turtle according to claim 8, it is characterized in that: carry out water quality management work between the breeding period of the outer pool, when water transparence is lower than 20cm, in time more renew water, quantity of exchanged water is 1/5～1/2 of former Chi Shui.
10. according to Claim 8 or the cultivation cultural method of 9 described black soft-shelled turtles, it is characterized in that: the outer pool is put Chi Shui in a suitable place to breed when pH value is lower than 7, splashes with quicklime water.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2008100608191A CN101248773B (en) | 2008-03-19 | 2008-03-19 | Breeding method of Wubie parent and breeding method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100608191A CN101248773B (en) | 2008-03-19 | 2008-03-19 | Breeding method of Wubie parent and breeding method thereof |
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| Publication Number | Publication Date |
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| CN101248773A CN101248773A (en) | 2008-08-27 |
| CN101248773B true CN101248773B (en) | 2011-06-08 |
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| CN2008100608191A Expired - Fee Related CN101248773B (en) | 2008-03-19 | 2008-03-19 | Breeding method of Wubie parent and breeding method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103734082A (en) * | 2013-12-20 | 2014-04-23 | 德清县吴越水产养殖有限公司 | Artificial cultivation method of three-way-cross soft-shelled turtle species |
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| CN102172229B (en) * | 2011-01-28 | 2012-11-07 | 绍兴中亚工贸园有限公司 | Process for germplasm breeding of soft-shelled turtles |
| CN102669013A (en) * | 2011-03-07 | 2012-09-19 | 宜兴市中亚淡水生态养殖专业合作社 | Method for ecologically culturing Chinese black turtle |
| CN102524138A (en) * | 2012-02-22 | 2012-07-04 | 福建省水产研究所 | Breeding method for selfing prior to hybridizing of whiteleg shrimp populations |
| CN104430065A (en) * | 2013-11-25 | 2015-03-25 | 融安县渔业技术推广站 | Breeding method of Chinese soft-shelled turtles |
| CN104381159B (en) * | 2014-09-30 | 2022-12-20 | 湖北宏旺生态农业科技股份有限公司 | Fine breed culture and breeding method for Chinese softshell turtles |
| CN106172186A (en) * | 2016-07-11 | 2016-12-07 | 全椒县志恒庄园 | A kind of artificial breeding method of Trionyx sinensis Wiegmann |
| CN107743922A (en) * | 2017-10-31 | 2018-03-02 | 广西平南县平原农牧有限公司 | A kind of yellow sand soft-shelled turtle and the hybridization breeding method of black bottom soft-shelled turtle |
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| CN103734082A (en) * | 2013-12-20 | 2014-04-23 | 德清县吴越水产养殖有限公司 | Artificial cultivation method of three-way-cross soft-shelled turtle species |
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| CN101248773A (en) | 2008-08-27 |
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