CN101245248A - Production of desertification land improvement material with steam shatter stalk solid-state fermentation - Google Patents

Production of desertification land improvement material with steam shatter stalk solid-state fermentation Download PDF

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Publication number
CN101245248A
CN101245248A CNA200810102982XA CN200810102982A CN101245248A CN 101245248 A CN101245248 A CN 101245248A CN A200810102982X A CNA200810102982X A CN A200810102982XA CN 200810102982 A CN200810102982 A CN 200810102982A CN 101245248 A CN101245248 A CN 101245248A
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solid state
state fermentation
steam
seed liquor
stalk
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CN100580054C (en
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陈洪章
宋永民
张建兴
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Jiangsu Senmao Ecological Technology Co., Ltd.
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Institute of Process Engineering of CAS
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Abstract

The invention relates to a preparation method of desertification land modified materials through steam shattering straws and solid state fermentation, which consists of the following steps, first, pretreatment is given to straws, thus obtaining the straw raw materials shattered by steam, after being shattered, straws are put together with wheat bran for solid state fermentation so as to produce functional materials with biological fertilizer and plant disease prevention and treatment, then the materials are remixed with degradable bacterial cellulose with strong water absorption. The products can improve the microstructure of soil, and reduce the using amount of active water retaining agent, the great amount of soil beneficial microorganism enriched has the functions of water storing, soil moisture conservation, and nutrient element slow releasing, etc., so the products are suitable for the mass application of the prevention and treatment of sand land, wherein, the organic content of the products is more than 60 percent, the content of humus is more than 15 percent, and the total number of effective living bacteria is more than 0.3 billion per gram.

Description

The steam explosive-crushing straw solid state fermentation prepares sandy land improvement material
Technical field
The present invention relates to a kind of method for preparing sandy land improvement material, particularly a kind of method for preparing sandy land improvement material with the steam explosive-crushing straw solid state fermentation
Background technology
Desertification of land is one of current the most serious ecological environment problem of China, the sandy land area is 173.97 ten thousand square kilometres, account for 18.12% of area, administer and the dynamics that improves, still be in improvement now and desertify the stage at a stalemate though China recent years has strengthened sandy land.Adopting stalk to carry out sandy land improvement begins to use from the nineties in last century in China, the main way at initial stage is to similar abroad, many employing stalks bury to be made anti-sand-protecting barrier or covers sand prevention, but owing to can not tackle the problem at its root, this application mode can only be very limited assist measure.
Because drought and water shortage, vegetation deterioration, soil texture difference are the main drives of desertification of land, therefore, Chinese scholars is recognized moisture self-restraint ability is improved, improved to the soil texture and Vegetation Construction is the essential measure of control desertification of land, and carried out big quantity research, the focus of applied chemistry material improvement once becoming research around these aspects.But because cost is too high, and plant is limited to the specific absorption of a large amount of chemical improvement agents, and long-term application not only can polluted underground water, and can cause soil compaction, destroys ecotope.Therefore, stalk is administered with the effect that improves in desertification of land and is much accounted of gradually again.Studying from existing, is that the sandy land of main raw material is improved the test effect of material and is not less than chemical material with the stalk, compares with simple chemical material to have advantages such as starting material are easy to get, cost is low, the lasting period is long, multiple functional.
At present, most of sandy land improvement materials of China or modifying agent often simply adopt the physical method of mechanical disintegration when utilizing stalk as additive, because pre-treatment is simpler, the stack retting cycle is longer when carrying out compost, product quality is relatively poor, and also fertilizer efficiency is low behind the field.The method that has (CN1534006A) is though from improving the angle of soil fertility, do not have obviously to improve the water-retaining capacity of soil, and the product processing energy consumption is higher; Adopt straw to prepare the report (CN1498941A) of biological activity water conservation modifying agent in addition, but the preparation of its active water-absorbing material needs chemical acid alkali to handle, can produce to a certain degree pollution environment.In addition, because straw nutrition component content imbalance often needs additionally to add a certain proportion of mineral manure, organic nitrogen source, humic acid and scarce metallic compound (CN1257855A) when being used for land reclamation, fundamentally do not reduce product cost.
Summary of the invention
[purpose of the present invention] to utilize stalk to prepare sandy land improvement material pretreatment technology undesirable at existing, there is environmental pollution, and the course of processing needs the extra too high problem of application cost that compositions such as inorganic fertilizer, humic acid, nutrition cause of adding, provide a kind of steam explosive-crushing straw solid state fermentation to prepare the method for sandy land improvement material, handle by steam explosion and solid state fermentation, improve the quality and the characteristic of stalk, the effect that increases bio-feritlizer reduces the technical costs of desertification of land control to reduce the consumption and the environmental pollution of chemical fertilizer.
[thinking of the present invention] steam explosive-crushing straw solid state fermentation provided by the invention prepares the method for sandy land improvement material, utilize the stalk fibre characteristic to make the functional product that is similar to the chemical modifying material, utilize physics and biological process that stalk is handled, adopt steam explosion and solid state fermentation to improve the water-absorbent of material respectively, the content of humify and nutritive element, then with degradable strong absorptive material---bacteria cellulose forms preparation under certain processing condition, not only can improve soil microstructure, obviously improve the soil water storage ability, reduce the consumption of bioactive water retaining agent, and a large amount of soil beneficial microorganisms of enrichment have and retain and conserve soil moisture behind the solid state fermentation, functions such as nutritive element slowly-releasing are suitable for the large-scale application that sandy land is prevented and treated.
[technological line of the present invention]
Provided by the inventionly prepare the method for sandy land improvement material with the steam explosive-crushing straw solid state fermentation, its technological line is:
1. the preparation of steam explosive-crushing straw raw material
To put in order strain stalk rough lumber to 3~5 centimetres, added water infiltration 10~20 minutes according to raw material weight in 1: 1~2 ratio, raw material is added in the steam-explosion jar, feed high pressure steam, at 1.5~1.7MPa, pressure was held time 4~8 minutes with the steam-explosion jar pressure-controlling, opened pressure in the bleeder valve venting jar then rapidly, stalk makes the stalk that steam explosion is handled from discharge port ejection and quick-fried broken;
2. composite microbial system solid state fermentation
A. the preparation of single strain seed liquor
The liquid nutrient medium of preparation viride, aztobacter sp, azotobacter vinelandii, phosphorus decomposing bacillus megaterium, frozen glue sample genus bacillus, trichoderma harziarum, get a ring lawn from each bacterial strain inclined-plane, insert liquid nutrient medium respectively, cultivate in 120 rev/mins of joltings, 25~28 ℃ of culture temperature, cultivated 2~3 days, and promptly got seed liquor;
B. composite microbial system solid state fermentation
With the steam explosive-crushing straw raw material pulverizing, cross 20 mesh sieves, add the wheat bran of its weight 15~25%, mixing and stirring promptly gets solid state fermentation matrix.
In solid state fermentation matrix, add water and stir, adjusting solid-to-liquid ratio is 1: 4~6, high pressure steam sterilization 20 minutes, insert the viride seed liquor of solid state fermentation matrix weight 4~6% then, place constant incubator, culture temperature is 25~28 ℃, cultivated 2~3 days, finish the aztobacter sp seed liquor that solid state fermentation matrix weight 5~7% is inserted in the back, 5~7% azotobacter vinelandii seed liquor, 4~6% phosphorus decomposing bacillus megaterium seed liquor, 4~6% frozen glue sample genus bacillus seed liquor, 5~7% trichoderma harziarum seed liquor is cultivated, culture temperature is 25~28 ℃, cultivates 4~6 days;
3. bacteria cellulose fermentation
Acetobacter xylinum is inserted the acetic bacteria liquid nutrient medium, inoculum size 2~4%, 28~30 ℃ leave standstill cultivation 7~9 days, but the proper extension fermentation period is to improve bacteria cellulose output.
4. after the fermentation ends solid state fermentation material is mixed by 1: 0.03~0.05 with bacteria cellulose, dry at normal temperatures, promptly make sandy land improvement material.
Described whole strain stalk is maize straw, wheat stalk, rice straw or rape stalk.
Described steam explosive-crushing straw is compared with the stalk that simple mechanical is pulverized, and its water-intake rate exceeds 32~40%, and water retention exceeds 80~100%.
After the described fermentation ends solid state fermentation material and bacteria cellulose being mixed by 1: 0.03~0.05 is dry weight basis with both.
Sandy land improvement material with the preparation of steam explosive-crushing straw solid state fermentation provided by the invention, organic content>60%, content of humic acid>15%, effective total viable count>300,000,000/gram, nitrogen, phosphorus, potassium content are all>5%.
Embodiment
Embodiment 1
1. the preparation of steam explosion maize straw
To put in order strain maize straw rough lumber to 3~5 centimetres, added water infiltration 15 minutes according to raw material weight in 1: 2 ratio, raw material is added in the steam-explosion jar, feed high pressure steam, at 1.5MPa, pressure was held time 7 minutes with the steam-explosion jar pressure-controlling, opened pressure in the bleeder valve venting jar then rapidly, stalk makes the maize straw raw material that steam explosion is handled from discharge port ejection and quick-fried broken;
2. composite microbial system solid state fermentation
A. the preparation of single strain seed liquor
The liquid nutrient medium of preparation viride, aztobacter sp, azotobacter vinelandii, phosphorus decomposing bacillus megaterium, frozen glue sample genus bacillus, trichoderma harziarum, get a ring lawn from each bacterial strain inclined-plane, insert liquid nutrient medium respectively, cultivate in 120 rev/mins of joltings, 28 ℃ of culture temperature, cultivated 2 days, and got seed liquor;
B. composite microbial system solid state fermentation
The steam explosion maize straw is pulverized, crossed 20 mesh sieves, add the wheat bran of its weight 15%, mixing and stirring promptly gets solid state fermentation matrix.
Add water and stir in solid state fermentation matrix, adjusting solid-to-liquid ratio is 1: 6, and high pressure steam sterilization 20 minutes inserts the viride seed liquor of solid state fermentation matrix weight 6% then, places constant incubator to cultivate 2 days for 28 ℃; The aztobacter sp seed liquor of access solid state fermentation matrix weight 7%, 7% azotobacter vinelandii seed liquor, 6% phosphorus decomposing bacillus megaterium seed liquor, 6% frozen glue sample genus bacillus seed liquor, 7% trichoderma harziarum seed liquor are cultivated after finishing, culture temperature is 28 ℃, cultivates 4 days;
3. bacteria cellulose fermentation
Acetobacter xylinum is inserted the acetic bacteria liquid nutrient medium, and inoculum size leaves standstill for 4%, 30 ℃ cultivated 7 days, but the proper extension fermentation period is to improve bacteria cellulose output.
4. after the fermentation ends solid state fermentation material is mixed by 1: 0.05 with bacteria cellulose, dry at normal temperatures, promptly make steam explosion maize straw sandy land improvement material.
With the sandy land improvement material of steam explosion maize straw preparation, organic content is 62%, and content of humic acid is 17%, and effectively total viable count is about 3.22 hundred million/gram, and nitrogen, phosphorus, potassium content are respectively 6.4%, 5.8%, 5.9%.
Embodiment 2
1. the preparation of steam explosion wheat stalk
To put in order strain wheat stalk rough lumber to 3~5 centimetres, added water infiltration 20 minutes according to raw material weight in 1: 1 ratio, raw material is added in the steam-explosion jar, feed high pressure steam, at 1.5MPa, pressure was held time 6 minutes with the steam-explosion jar pressure-controlling, opened pressure in the bleeder valve venting jar then rapidly, stalk makes the wheat stalk raw material that steam explosion is handled from discharge port ejection and quick-fried broken;
2. composite microbial system solid state fermentation
A. the preparation of single strain seed liquor
The liquid nutrient medium of preparation viride, aztobacter sp, azotobacter vinelandii, phosphorus decomposing bacillus megaterium, frozen glue sample genus bacillus, trichoderma harziarum, get a ring lawn from each bacterial strain inclined-plane, insert liquid nutrient medium respectively, cultivate in 120 rev/mins of joltings, 25 ℃ of culture temperature, cultivated 3 days, and got seed liquor;
B. composite microbial system solid state fermentation
The steam explosion wheat stalk is pulverized, crossed 20 mesh sieves, add the wheat bran of its weight 20%, mixing and stirring promptly gets solid state fermentation matrix.
Add water and stir in solid state fermentation matrix, adjusting solid-to-liquid ratio is 1: 5, and high pressure steam sterilization 20 minutes inserts the viride seed liquor of solid state fermentation matrix weight 5% then, places constant incubator to cultivate 3 days for 28 ℃; The aztobacter sp seed liquor of access solid state fermentation matrix weight 5%, 5% azotobacter vinelandii seed liquor, 5% phosphorus decomposing bacillus megaterium seed liquor, 5% frozen glue sample genus bacillus seed liquor, 6% trichoderma harziarum seed liquor are cultivated after finishing, culture temperature is 25 ℃, cultivates 6 days;
3. bacteria cellulose fermentation
Acetobacter xylinum is inserted the acetic bacteria liquid nutrient medium, and inoculum size leaves standstill for 2%, 30 ℃ cultivated 9 days, but the proper extension fermentation period is to improve bacteria cellulose output.
4. after the fermentation ends solid state fermentation material is mixed by 1: 0.03 with bacteria cellulose, dry at normal temperatures, promptly make steam explosion wheat stalk sandy land improvement material.
With the sandy land improvement material of steam explosion wheat stalk preparation, organic content is 61%, and content of humic acid is 16%, and effectively total viable count is about 3.36 hundred million/gram, and nitrogen, phosphorus, potassium content are respectively 6.1%, 5.6%, 5.5%.
Embodiment 3
1. the preparation of steam explosion rice straw
To put in order strain rice straw rough lumber to 3~5 centimetres, added water infiltration 20 minutes according to raw material weight in 1: 2 ratio, raw material is added in the steam-explosion jar, feed high pressure steam, at 1.7MPa, pressure was held time 5 minutes with the steam-explosion jar pressure-controlling, opened pressure in the bleeder valve venting jar then rapidly, stalk makes the rice straw raw material that steam explosion is handled from discharge port ejection and quick-fried broken;
2. composite microbial system solid state fermentation
A. the preparation of single strain seed liquor
The liquid nutrient medium of preparation viride, aztobacter sp, azotobacter vinelandii, phosphorus decomposing bacillus megaterium, frozen glue sample genus bacillus, trichoderma harziarum, get a ring lawn from each bacterial strain inclined-plane, insert liquid nutrient medium respectively, cultivate in 120 rev/mins of joltings, 26 ℃ of culture temperature, cultivated 3 days, and got seed liquor;
B. composite microbial system solid state fermentation
The steam explosion rice straw is pulverized, crossed 20 mesh sieves, add the wheat bran of its weight 25%, mixing and stirring promptly gets solid state fermentation matrix.
Add water and stir in solid state fermentation matrix, adjusting solid-to-liquid ratio is 1: 4, and high pressure steam sterilization 20 minutes inserts the viride seed liquor of solid state fermentation matrix weight 5% then, places constant incubator to cultivate 3 days for 28 ℃; The aztobacter sp seed liquor of access solid state fermentation matrix weight 5%, 7% azotobacter vinelandii seed liquor, 4% phosphorus decomposing bacillus megaterium seed liquor, 6% frozen glue sample genus bacillus seed liquor, 5% trichoderma harziarum seed liquor are cultivated after finishing, culture temperature is 26 ℃, cultivates 6 days;
3. bacteria cellulose fermentation
Acetobacter xylinum is inserted the acetic bacteria liquid nutrient medium, and inoculum size leaves standstill for 3%, 30 ℃ cultivated 8 days, but the proper extension fermentation period is to improve bacteria cellulose output.
4. after the fermentation ends solid state fermentation material is mixed by 1: 0.04 with bacteria cellulose, dry at normal temperatures, promptly make steam explosion rice straw sandy land improvement material.
With the sandy land improvement material of steam explosion rice straw preparation, organic content is 64%, and content of humic acid is 15%, and effectively total viable count is about 3.17 hundred million/gram, and nitrogen, phosphorus, potassium content are respectively 6.1%, 5.4%, 5.9%.
Embodiment 4
1. the preparation of steam explosion rape stalk
To put in order strain rape stalk rough lumber to 3~5 centimetres, added water infiltration 15 minutes according to raw material weight in 1: 1 ratio, raw material is added in the steam-explosion jar, feed high pressure steam, at 1.5MPa, pressure was held time 8 minutes with the steam-explosion jar pressure-controlling, opened pressure in the bleeder valve venting jar then rapidly, stalk makes the rape stalk raw material that steam explosion is handled from discharge port ejection and quick-fried broken;
2. composite microbial system solid state fermentation
A. the preparation of single strain seed liquor
The liquid nutrient medium of preparation viride, aztobacter sp, azotobacter vinelandii, phosphorus decomposing bacillus megaterium, frozen glue sample genus bacillus, trichoderma harziarum, get a ring lawn from each bacterial strain inclined-plane, insert liquid nutrient medium respectively, cultivate in 120 rev/mins of joltings, 27 ℃ of culture temperature, cultivated 2 days, and got seed liquor;
B. composite microbial system solid state fermentation
The steam explosion rape stalk is pulverized, crossed 20 mesh sieves, add the wheat bran of its weight 20%, mixing and stirring promptly gets solid state fermentation matrix.
Add water and stir in solid state fermentation matrix, adjusting solid-to-liquid ratio is 1: 6, and high pressure steam sterilization 20 minutes inserts the viride seed liquor of solid state fermentation matrix weight 5% then, places constant incubator to cultivate 2 days for 28 ℃; The aztobacter sp seed liquor of access solid state fermentation matrix weight 6%, 6% azotobacter vinelandii seed liquor, 4% phosphorus decomposing bacillus megaterium seed liquor, 4% frozen glue sample genus bacillus seed liquor, 6% trichoderma harziarum seed liquor are cultivated after finishing, culture temperature is 27 ℃, cultivates 5 days;
3. bacteria cellulose fermentation
Acetobacter xylinum is inserted the acetic bacteria liquid nutrient medium, and inoculum size leaves standstill for 4%, 30 ℃ cultivated 8 days, but the proper extension fermentation period is to improve bacteria cellulose output.
4. after the fermentation ends solid state fermentation material is mixed by 1: 0.03 with bacteria cellulose, dry at normal temperatures, promptly make steam explosion rape stalk sandy land improvement material.
With the sandy land improvement material of steam explosion rape stalk preparation, organic content is 62%, and content of humic acid is 18%, and effectively total viable count is about 3.41 hundred million/gram, and nitrogen, phosphorus, potassium content are respectively 5.8%, 5.3%, 5.5%.

Claims (4)

1. the steam explosive-crushing straw solid state fermentation prepares sandy land improvement material, and its step is as follows:
1) preparation of steam explosive-crushing straw raw material
To put in order strain stalk rough lumber to 3~5 centimetres, added water infiltration 10~20 minutes according to raw material weight in 1: 1~2 ratio, raw material is added in the steam-explosion jar, feed high pressure steam, at 1.5~1.7MPa, pressure was held time 4~8 minutes with the steam-explosion jar pressure-controlling, opened pressure in the bleeder valve venting jar then rapidly, stalk makes the stalk that steam explosion is handled from discharge port ejection and quick-fried broken;
2) composite microbial system solid state fermentation
A. the preparation of single strain seed liquor
The liquid nutrient medium of preparation viride, aztobacter sp, azotobacter vinelandii, phosphorus decomposing bacillus megaterium, frozen glue sample genus bacillus, trichoderma harziarum, get a ring lawn from each bacterial strain inclined-plane, insert liquid nutrient medium respectively, cultivate in 120 rev/mins of joltings, 25~28 ℃ of culture temperature, cultivated 2~3 days, and promptly got seed liquor;
B. composite microbial system solid state fermentation
With the steam explosive-crushing straw raw material pulverizing, cross 20 mesh sieves, add the wheat bran of its weight 15~25%, mixing and stirring promptly gets solid state fermentation matrix.
In solid state fermentation matrix, add water and stir, adjusting solid-to-liquid ratio is 1: 4~6, high pressure steam sterilization 20 minutes, insert the viride seed liquor of solid state fermentation matrix weight 4~6% then, place constant incubator, culture temperature is 25~28 ℃, cultivated 2~3 days, finish the aztobacter sp seed liquor that solid state fermentation matrix weight 5~7% is inserted in the back, 5~7% azotobacter vinelandii seed liquor, 4~6% phosphorus decomposing bacillus megaterium seed liquor, 4~6% frozen glue sample genus bacillus seed liquor, 5~7% trichoderma harziarum seed liquor is cultivated, culture temperature is 25~28 ℃, cultivates 4~6 days;
3) bacteria cellulose fermentation
Acetobacter xylinum is inserted the acetic bacteria liquid nutrient medium, inoculum size 2~4%, 28~30 ℃ leave standstill cultivation 7~9 days, but the proper extension fermentation period is to improve bacteria cellulose output.
4) after the fermentation ends solid state fermentation material is mixed by 1: 0.03~0.05 with bacteria cellulose, dry at normal temperatures, promptly make steam explosive-crushing straw sandy land improvement material.
2. steam explosive-crushing straw solid state fermentation as claimed in claim 1 prepares sandy land improvement material, and it is characterized in that: described whole strain stalk is maize straw, wheat stalk, rice straw or rape stalk.
3. steam explosive-crushing straw solid state fermentation as claimed in claim 1 prepares sandy land improvement material, it is characterized in that: described steam explosive-crushing straw is compared with the stalk that simple mechanical is pulverized, and its water-intake rate exceeds 32~40%, and water retention exceeds 80~100%.
4. steam explosive-crushing straw solid state fermentation as claimed in claim 1 prepares sandy land improvement material, it is characterized in that: after the described fermentation ends solid state fermentation material and bacteria cellulose being mixed by 1: 0.03~0.05 is dry weight basis with both.
CN200810102982A 2008-03-28 2008-03-28 Production of desertification land improvement material with steam shatter stalk solid-state fermentation Expired - Fee Related CN100580054C (en)

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101851125A (en) * 2009-03-30 2010-10-06 杨春和 Agricultural waste-composting treatment method
CN101743790B (en) * 2009-12-10 2011-05-11 四川大学 Soil water ecological effectiveness control method
CN102219605A (en) * 2011-04-29 2011-10-19 强光初 Plant culture medium
CN102226030A (en) * 2011-05-25 2011-10-26 无锡凯恩科特再生能源科技有限公司 Preparation method of crop straw based high power water absorbent
CN102234670A (en) * 2010-04-20 2011-11-09 中国科学院过程工程研究所 Method for producing bacterial cellulose through solid state fermentation by using inert adsorption carrier
CN112028714A (en) * 2020-09-15 2020-12-04 辽宁工程技术大学 Active nutrient restoration agent for desertification soil and preparation method thereof
CN112537990A (en) * 2020-12-21 2021-03-23 西藏自治区农牧科学院农业资源与环境研究所 Green manure for dry land and preparation method thereof
CN112609489A (en) * 2020-11-24 2021-04-06 湖南垒土农业科技发展有限公司 Wood crude fiber modified based on fulvic acid and/or humic acid and preparation method and application thereof

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CN1504104A (en) * 2002-11-28 2004-06-16 中国科学院过程工程研究所 Preparation method of plant stalk feed and organic feed by fixing nitrogen in stalk
CN101066092A (en) * 2007-05-23 2007-11-07 张培举 Stalk microbe feed additive and its prepn
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101851125A (en) * 2009-03-30 2010-10-06 杨春和 Agricultural waste-composting treatment method
CN101743790B (en) * 2009-12-10 2011-05-11 四川大学 Soil water ecological effectiveness control method
CN102234670A (en) * 2010-04-20 2011-11-09 中国科学院过程工程研究所 Method for producing bacterial cellulose through solid state fermentation by using inert adsorption carrier
CN102234670B (en) * 2010-04-20 2014-06-04 中国科学院过程工程研究所 Method for producing bacterial cellulose through solid state fermentation by using inert adsorption carrier
CN102219605A (en) * 2011-04-29 2011-10-19 强光初 Plant culture medium
CN102226030A (en) * 2011-05-25 2011-10-26 无锡凯恩科特再生能源科技有限公司 Preparation method of crop straw based high power water absorbent
CN102226030B (en) * 2011-05-25 2012-11-07 无锡凯恩科特再生能源科技有限公司 Preparation method of crop straw based high power water absorbent
CN112028714A (en) * 2020-09-15 2020-12-04 辽宁工程技术大学 Active nutrient restoration agent for desertification soil and preparation method thereof
CN112609489A (en) * 2020-11-24 2021-04-06 湖南垒土农业科技发展有限公司 Wood crude fiber modified based on fulvic acid and/or humic acid and preparation method and application thereof
CN112537990A (en) * 2020-12-21 2021-03-23 西藏自治区农牧科学院农业资源与环境研究所 Green manure for dry land and preparation method thereof

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Granted publication date: 20100113

Termination date: 20140328