CN101230106B - 香蕉多糖的制备方法 - Google Patents
香蕉多糖的制备方法 Download PDFInfo
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- CN101230106B CN101230106B CN200810009334XA CN200810009334A CN101230106B CN 101230106 B CN101230106 B CN 101230106B CN 200810009334X A CN200810009334X A CN 200810009334XA CN 200810009334 A CN200810009334 A CN 200810009334A CN 101230106 B CN101230106 B CN 101230106B
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- CN
- China
- Prior art keywords
- banana
- preparation
- banana polysaccharide
- precipitation
- polysaccharide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 235000018290 Musa x paradisiaca Nutrition 0.000 title claims abstract description 57
- 150000004676 glycans Chemical class 0.000 title claims abstract description 48
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 48
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 240000005561 Musa balbisiana Species 0.000 title 1
- 241000234295 Musa Species 0.000 claims abstract description 57
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 36
- 230000007062 hydrolysis Effects 0.000 claims abstract description 20
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 20
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000000034 method Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 12
- 239000012043 crude product Substances 0.000 claims abstract description 8
- 239000012530 fluid Substances 0.000 claims abstract description 8
- 239000002253 acid Substances 0.000 claims abstract description 7
- 238000004587 chromatography analysis Methods 0.000 claims abstract description 7
- 239000000706 filtrate Substances 0.000 claims abstract description 7
- 239000012528 membrane Substances 0.000 claims abstract description 7
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 claims abstract description 6
- 238000001556 precipitation Methods 0.000 claims description 26
- 239000000243 solution Substances 0.000 claims description 16
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 9
- 239000000872 buffer Substances 0.000 claims description 7
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 claims description 7
- 239000002953 phosphate buffered saline Substances 0.000 claims description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 6
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- 239000000284 extract Substances 0.000 claims description 6
- 239000002002 slurry Substances 0.000 claims description 6
- 238000001291 vacuum drying Methods 0.000 claims description 6
- 108090000526 Papain Proteins 0.000 claims description 5
- 239000004365 Protease Substances 0.000 claims description 5
- 229940055729 papain Drugs 0.000 claims description 5
- 235000019834 papain Nutrition 0.000 claims description 5
- 238000012546 transfer Methods 0.000 claims description 5
- 108010019160 Pancreatin Proteins 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 4
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 4
- 239000003513 alkali Substances 0.000 claims description 4
- 238000013375 chromatographic separation Methods 0.000 claims description 4
- 230000014759 maintenance of location Effects 0.000 claims description 4
- 229940055695 pancreatin Drugs 0.000 claims description 4
- 206010033546 Pallor Diseases 0.000 claims description 3
- 238000010009 beating Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 239000004744 fabric Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 3
- 235000011121 sodium hydroxide Nutrition 0.000 claims description 3
- 239000006228 supernatant Substances 0.000 claims description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 2
- AXCZMVOFGPJBDE-UHFFFAOYSA-L calcium dihydroxide Chemical compound [OH-].[OH-].[Ca+2] AXCZMVOFGPJBDE-UHFFFAOYSA-L 0.000 claims description 2
- 239000000920 calcium hydroxide Substances 0.000 claims description 2
- 229910001861 calcium hydroxide Inorganic materials 0.000 claims description 2
- 235000011116 calcium hydroxide Nutrition 0.000 claims description 2
- 235000013372 meat Nutrition 0.000 claims description 2
- 235000011118 potassium hydroxide Nutrition 0.000 claims description 2
- 229910000030 sodium bicarbonate Inorganic materials 0.000 claims description 2
- 235000017557 sodium bicarbonate Nutrition 0.000 claims description 2
- 238000000967 suction filtration Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims 3
- 238000007710 freezing Methods 0.000 claims 2
- 230000008014 freezing Effects 0.000 claims 2
- 238000005406 washing Methods 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 abstract description 6
- 102000004190 Enzymes Human genes 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000000605 extraction Methods 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 abstract description 4
- 235000019441 ethanol Nutrition 0.000 abstract 2
- 230000002779 inactivation Effects 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 description 5
- 238000000746 purification Methods 0.000 description 3
- 208000030507 AIDS Diseases 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 102000035101 Aspartic proteases Human genes 0.000 description 1
- 108091005502 Aspartic proteases Proteins 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 241001529936 Murinae Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012445 acidic reagent Substances 0.000 description 1
- 235000021015 bananas Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- AZHSSKPUVBVXLK-UHFFFAOYSA-N ethane-1,1-diol Chemical compound CC(O)O AZHSSKPUVBVXLK-UHFFFAOYSA-N 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 238000009776 industrial production Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000002547 new drug Substances 0.000 description 1
- 235000011837 pasties Nutrition 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 239000008215 water for injection Substances 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Claims (11)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN200810009334XA CN101230106B (zh) | 2008-02-21 | 2008-02-21 | 香蕉多糖的制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN200810009334XA CN101230106B (zh) | 2008-02-21 | 2008-02-21 | 香蕉多糖的制备方法 |
Publications (2)
Publication Number | Publication Date |
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CN101230106A CN101230106A (zh) | 2008-07-30 |
CN101230106B true CN101230106B (zh) | 2010-06-09 |
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Family Applications (1)
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CN200810009334XA Expired - Fee Related CN101230106B (zh) | 2008-02-21 | 2008-02-21 | 香蕉多糖的制备方法 |
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CN (1) | CN101230106B (zh) |
Families Citing this family (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103360506B (zh) * | 2013-07-18 | 2015-10-28 | 华南理工大学 | 一种香蕉低聚糖的提取方法 |
JP6523647B2 (ja) * | 2013-11-14 | 2019-06-05 | 大谷 勝 | バナナ由来組成物の製造方法 |
CN103947968A (zh) * | 2014-04-30 | 2014-07-30 | 桂林军供生化技术开发有限公司 | 一种香蕉提取物及其制备方法及应用 |
CN105924541B (zh) * | 2016-05-04 | 2018-04-17 | 中国科学院华南植物园 | 一种制备α‑1,6‑葡聚糖的方法 |
CN108142825A (zh) * | 2017-12-22 | 2018-06-12 | 广西兴业百谷米业有限公司 | 一种高含量的膳食纤维米及其制备方法 |
CN110464872B (zh) * | 2019-09-26 | 2020-09-04 | 海南医学院 | 一种医用水凝胶及其制备方法和应用 |
CN114230682B (zh) * | 2022-02-08 | 2022-08-19 | 中国热带农业科学院分析测试中心 | 一种功能性香蕉多糖bpf2及其制备与应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1337410A (zh) * | 2001-09-03 | 2002-02-27 | 王维新 | 从水果中提取水果多糖产品的生产方法 |
CN101015336A (zh) * | 2007-03-09 | 2007-08-15 | 伍曾利 | 椰子提取物的制备方法 |
-
2008
- 2008-02-21 CN CN200810009334XA patent/CN101230106B/zh not_active Expired - Fee Related
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1337410A (zh) * | 2001-09-03 | 2002-02-27 | 王维新 | 从水果中提取水果多糖产品的生产方法 |
CN101015336A (zh) * | 2007-03-09 | 2007-08-15 | 伍曾利 | 椰子提取物的制备方法 |
Non-Patent Citations (2)
Title |
---|
沈建林等.香蕉多糖的分离纯化及其性质研究.食品科学第25卷 第8期.2004,第25卷(第8期),第73-75页. |
沈建林等.香蕉多糖的分离纯化及其性质研究.食品科学第25卷 第8期.2004,第25卷(第8期),第73-75页. * |
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Publication number | Publication date |
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CN101230106A (zh) | 2008-07-30 |
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Owner name: HAINAN YETAI BIOLOGY ENGINEERING CO., LTD. Free format text: FORMER OWNER: WU ZENGLI Effective date: 20091218 |
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Effective date of registration: 20091218 Address after: North Building No. 3 Hainan province Haikou DeMax Rhine Wenhua Road, room 702, post encoding: 570203 Applicant after: Hainan Yietai Bio-Engineering Co., Ltd. Address before: Xinhai town Xixiu Xiuying District village of Hainan Province, Haikou City, No. 053 post encoding: 570312 Applicant before: Wu Zengli |
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Inventor after: Han Jinguang Inventor after: Wu Zengli Inventor before: Wu Zengli |
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Effective date of registration: 20100414 Address after: 570000 Hainan city in Haikou Province, the national science and technology road high tech Zone Yuke Building Room 518 Applicant after: Hainan Stand Bio-Technology Co., Ltd. Address before: No. 3 North Rhine 570203 Hainan city of Haikou Province Yu Wenhua Road Room 702 Applicant before: Hainan Yietai Bio-Engineering Co., Ltd. |
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Address after: Trough of Haikou national hi tech Industrial Development Zone, two cross road 570311 Hainan city of Haikou province No. 10 Patentee after: Hainan standard biological Polytron Technologies Inc Address before: 570000 Hainan city in Haikou Province, the national science and technology road high tech Zone Yuke Building Room 518 Patentee before: Hainan Stand Bio-Technology Co., Ltd. |
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Denomination of invention: Preparation method of banana polysaccharide Effective date of registration: 20171122 Granted publication date: 20100609 Pledgee: Wenchang Hainan rural commercial bank Limited by Share Ltd Pledgor: Hainan standard biological Polytron Technologies Inc Registration number: 2017460000010 |
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