CN101194715B - Strong nutrient solution - Google Patents
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- CN101194715B CN101194715B CN200710156348XA CN200710156348A CN101194715B CN 101194715 B CN101194715 B CN 101194715B CN 200710156348X A CN200710156348X A CN 200710156348XA CN 200710156348 A CN200710156348 A CN 200710156348A CN 101194715 B CN101194715 B CN 101194715B
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Abstract
The invention belongs to the category of nutritional health food, in particular to a strong nutrient solution which has rich nourishment, easy absorption, and all-around effect, and the invention is characterized in that the nutrient solution is made form the following animal and vegetable raw materials: (part by weight) Gynostemma pentaphylla 35-52, quail 24-38, red date 6-10, wolfberry fruit 4-7, haw 3-5, Oplopanax elatus 2-4 and Arrilus longan 2-5. The nutrient solution of the invention carries out the enzymolysis to the Oplopanax elatus and quail through adopting bioengineering technology, which can obtain effective animal protein and various amino acids and reserve the nourishment and effective elements at a maximum limit. The invention has the health function of fatigue resistance if taking for a long time.
Description
Technical field
The invention belongs to the nutritional health food category, particularly relate to a kind of strong nutrient solution.
Background technology
In the prior art, nutritional health food often with simple utilization directly extract, common process such as preparation processes, loss of effective components is big, it is slow to absorb, effect is single, is difficult to adapt to people's consumption requirement.
Summary of the invention
The object of the present invention is to provide a kind of nutritious, be easy to absorb, the comprehensive effects strong nutrient solution.
Solution of the present invention is based on understanding and the nourishing principle of motherland's medical science to physique strengthening and intelligence benefiting, nourishing healthy mechanism; With reference to modern nourishing healthy achievement in research; From motherland medicine treasure-house, filter out spleen benefiting and stimulating the appetite, phlegm reduction of blood circulation promoting, fill blood, set upright reinforcing, the natural edible animals and plants of herbal cuisine homology are raw material, according to the theory of traditional Chinese medical science prescription; Skim the cream off milk, make that its performance is built up health, the health-care effect of antifatigue.
Nutrient solution of the present invention is to adopt the animal-plant material of following component to process: (weight portion):
Gynostemma pentaphylla: 35-52; Quail: 24-38; Red date: 6-10; The fruit of Chinese wolfberry: 4-7;
Hawthorn: 3-5; Stichopus japonicus: 2-4; Arillus longan: 2-5.
Gynostemma pentaphylla among the present invention [gynostemma pentaphyllum (thunb) mak] is dicotyledonous guiding principle cucurbitaceae genus gynostemma.Chinese medicine is called seven leaf courages.Include multiple saponin, vitamin and amino acid to the human body beneficial.Research shows, the gynostemma pentaphylla active ingredient has reducing blood lipid, hypoglycemic, antitumor, anti-ageing, protection liver and strengthens effects such as body immune function.Illnesss such as Cardiovascular system disorders such as hyperlipidemia, hypertension, coronary heart disease, diabetes, tumour had good prevention effect.
Quail among the present invention is the meat of Phasianidae animal quail, and nature and flavor are sweet, flat, have beneficial gas qi-restoratives; Thick intestines end dysentery; The clearing damp of dispeling the wind, a surname's lung dampness removing is cut the long-pending function of removing infantile malnutrition due to digestive disturbances or intestinalparasites; Normal food, to neurasthenia, vascular sclerosis, pulmonary tuberculosis, malnutrition, bronchitis, limbs fatigue, etc. better curative effect all arranged.
Red date among the present invention is for belonging to the drying and ripening dry fruit of Li Ke plant jujube Ziziphus jujuba Mill.; The little perfume (or spice) of its gas, it is sweet to distinguish the flavor of; Nature and flavor with return through: sweet, temperature; Return spleen, stomach warp; Have tonifying middle-Jiao and Qi, the function of nourishing blood and tranquilization, it is few to be used to treat insufficiency of the spleen food, weak loose stool, hysteria of womam's symptom.
The fruit of Chinese wolfberry among the present invention is the dry mature fruit of plant of Solanaceae lycium barbarum Lycium barbarum L., and summer, two season of autumn gather when fruit is orange red, dry in the air to rhicnosis, tan by the sun again stiff to crust, pulp is soft, removes carpopodium; Or the hot blast low temperature drying, remove the fruit body of carpopodium; Its proterties is an odorless, sweet, little acid of distinguishing the flavor of; Nature and flavor with return through: sweet, flat; Return liver, kidney channel.Have nourishing liver and kidney, the effect of benefiting shrewd head; It is thanks to smart to be used for consumptive disease, soreness of waist and knee joint, and dizzy tinnitus, interior heat is quenched one's thirst, and the deficiency of blood is sallow, the treatment that blurred vision is not clear.
Hawthorn among the present invention has another name called the azalea fruit, and large-fruited Chinese hawthorn is the fruit of rosaceous plant hawthorn; China's most of areas all has distribution, and hawthorn does medicinal with fruit, slightly warm in nature; Flavor acid is sweet, goes into spleen, stomach, Liver Channel, food digesting stomach fortifying is arranged, activate blood circulation and disperse blood clots, restrain the only function of dysentery.To the long-pending phlegm and retained fluid of meat, the full acid regurgitation of ruffian, rush down dysentery intestines wind, pain in the back hernia, postpartum postpartum abdominal pain, lochiorrhagia, children's's breast food and stagnate etc., all effective in cure.
Stichopus japonicus among the present invention is a kind of of sea cucumber.Its nutritive value is high, and stichopus japonicus is classified as one of " eight delicacies " as a kind of seafood delights of preciousness.According to putting down in writing in the supplementary Amplifications of the Compendium of Materia Medica, " sea cucumber of east area of the Liao River, coextensive with eastern and southern Liaoning Province product, body colour is dark brown, and the tender thorniness of meat is referred to as the Liao Dynasty's ginseng or sea cucumber, quality is splendid, and the sweet temperature of the property of medicine is nontoxic, has tonifying kidney-yin, gives birth to arteries and veins blood, controls the inferior effects such as dysentery and ulcer ".Because of its property of medicine temperature compensation, so sufficient enemy's ginseng is the name sea cucumber.
Arillus longan among the present invention has another name called dried longan pulp, and it is nutritious, contains various carbohydrates and vitamin.It is warm in nature, flavor is sweet.Go into the heart, the spleen channel.Have the bushing spleen, control splenasthenic diarrhea, puffiness; Fill blood; Calm the nerves, control insomnia, forgetful, the palpitation with fear effect; The people who is suitable for the elderly, postpartum weakness, insufficiency of vital energy and blood or malnutrition, anaemia is edible; Also be fit to the treatment of neurasthenia and symptoms such as forgetful, the decrease of memory that causes, insomnia thereof.
Easy to carry, advantages of easy digesting, nutritious that nutrient solution of the present invention has; Particularly adopt biotechnology that stichopus japonicus and quail are carried out enzymolysis; Can obtain effective animal protein and several amino acids composition; Preserved nutrition and functional component to greatest extent, taken health-care effect for a long time with antifatigue.
The specific embodiment
Nutrient solution of the present invention is that component is processed by following raw materials according: (weight portion)
Gynostemma pentaphylla: 35-52; Quail: 24-38; Red date: 6-10; The fruit of Chinese wolfberry: 4-7;
Hawthorn: 3-5; Stichopus japonicus: 2-4; Arillus longan: 2-5.
Prescription preferred weight (part) ratio range of preparation nutrient solution of the present invention is:
Gynostemma pentaphylla: 40-48; Quail: 27-35; Red date: 7-9; The fruit of Chinese wolfberry: 4-7;
Hawthorn: 3-5; Stichopus japonicus: 3-4; Arillus longan: 2-4.
The optimum weight of this nutrient solution (part) proportioning is:
Gynostemma pentaphylla: 44; Quail: 32; Red date: 8; The fruit of Chinese wolfberry: 6; Hawthorn: 3;
Stichopus japonicus: 3; Arillus longan: 4.
It is following that said components is that raw material is processed the production method of nutrient solution of the present invention:
1, will sting participation quail enzymolysis earlier; Get stichopus japonicus and soak bloatedly, put into the enzymolysis pot with the quail of cleaning chopping then, adds an amount of water, and add a certain amount of papain, in 40-60 Celsius ℃ temperature, be incubated enzymolysis 6-8 hour, can obtain hydrolyzed protein liquid;
2, extract the active ingredient of other plant material again: get hawthorn, arillus longan, the fruit of Chinese wolfberry; Red date, Gynostemma pentaphylla is put into multi-functional extraction pot after the cleaning; Carry out second extraction active ingredient according to the Chinese medicine preparation rule, 1.5 hours for the first time, 1 hour for the second time; Temperature is controlled at 60-100 ℃, can obtain the extract of above-mentioned plant material;
3, hydrolyzed protein liquid that obtains and plant material extract are got final product by common process filter just, mixing, constant volume, smart filter, can, sterilization, lamp inspection, packing.
Every oral liquid loading amount is 10ml, and the dosage that is used to keep healthy is: be grown up every day twice, each 1, one month is a course of treatment, can take throughout the year.
Below further specify the beneficial effect of nutrient solution of the present invention through test, Test Example has comprised the toxicological experiment and the long term toxicity test of nutrient solution of the present invention.
Experimental example 1: toxicological experiment
Experiment purpose: observe nutrient solution of the present invention and give acute toxicity reflection and the death condition that mouse is taken the back generation.
Experiment material: animal used as test is the ICR mouse, is provided by Zhejiang Province's Experimental Animal Center.
Experimental technique and result:
1, LD
50Measure: get 40 of body weight 19-21gICR mouse, male and female half and half are divided into 4 groups immediately, and 10 every group, mouse supplies drinking-water, fasting 16 hours, and oral liquid dosage of the present invention is 8-40ml/kg, the agent distance is 10ml.The administration capacity is the 0.4ml/10g body weight, behind the gastric infusion, observes animal immediately and reports situations successively, and observe once every day, continuous 7 days, and write down toxicity day by day and report situations and the animal dead number, the result sees table 1.
Table 1. nutrient solution of the present invention is to the acute toxicity test of mouse
| Group | Dosage ml/kg | Number of animals (only) | Death toll (only) | The death rate (%) |
| 1 | 40 | 10 | 0 | 0 |
| 2 | 30 | 10 | 0 | 0 |
| 3 | 20 | 10 | 0 | 0 |
| 4 | 8 | 10 | 0 | 0 |
Four dose groups mouse of nutrient solution administration of the present invention all do not have death, also do not have poisoning symptom and occur, and 40ml/kg is nutrient solution Cmax of the present invention and heap(ed) capacity, no animal dead, LD
50Can't record, therefore carry out the maximal tolerated dose test.
2. maximal tolerated dose test
Get 48 of ICR mouse, body weight 19-21g, male and female half and half, mouse supplies drinking-water; Fasting 16 hours is divided into two groups immediately, once irritates stomach 40ml/kg for one group, one group with same dose give secondary (the upper and lower noon each once; 4 hours at interval), accumulated dose is 80ml/kg, is equivalent to 200 times of people's consumption.Observed 7 days continuously, the record mouse toxicity report situations (comprise excitement, uneasiness, tremble, convulsions, abnormal gait, shed tears, hydrostomia, breathing, ight soil) and death toll.Not observing any bad reaction and animal after the administration occurs dead.The maximal tolerance dose that shows nutrient solution of the present invention is greater than 40ml/kg (be equivalent to people's consumption 100 times).
Brief summary: nutrient solution of the present invention once irritates stomach for mouse, can not survey median lethal dose.Irritate the stomach maximal tolerance dose greater than 40ml/kg, this dosage is equivalent to 100 times of people's clinical dosage (10ml/ props up, 2/day calculating).
Experimental example 2: to the long term toxicity test of dog
Experiment purpose: observe the toxicity and the order of severity thereof that Beagle dog continuous irrigation stomach nutrient solution of the present invention was produced in 90 days, understand target organ and recovery and development that this nutrient solution causes toxic reaction, to confirm
The safe range of clinical this nutrient solution of use and dosage.
Experiment material: animal used as test is the Beagle dog, the 6-7 monthly age, and the male 8.2 ± 1.0kg of body weight, female body weight 6.6 ± 0.5kg is provided by Zhejiang Province's Experimental Animal Center.
Experimental technique:
(1) dosage and grouping
Two dose groups of experimental design and a control group, low dose group is 8ml/kg, high dose group is 40ml/kg, is equivalent to 20 times and 100 times of clinical people's consumption respectively.Nutrient solution of the present invention is regularly being fed every day
Once mix in food orally before the food, successively administration is 90 days.Dog is raised and train a week before the experiment, every dog one cage, random packet, 4 of every group of dogs, male and female half and half, every day quantitatively, circadian feeding, weekly by body weight adjustment dosage.
(2) inspection item
1. overview: (1) food ration and body weight: weigh weekly once, observe the variation of ingesting; (2) outward appearance sign and behavioral activity: observe the behavioral activity that dog has situation of change such as what is felt sick, vomiting, hydrostomia, breathing, ight soil and dog every day.
2. test item:
(1) electrocardiographic examination: the II ECG that leads.
(2) hematological indices: red blood cell, reticulocyte count, hemoglobin, total white blood cells and classification thereof, blood platelet and 2 blood times.
(3) blood parameters: the variation of measure asparatate aminotransferase (AST), ALT (ALT), sealing acid phosphatase (ALP), urea ammonia (BUN), total protein (TP), albumin (ALB), blood sugar (GLU), total bilirubin (T-BIL), flesh liver (Grea), T-CHOL (T-CHO) etc.
(4) system becomes celestial and histopathologic examination: in take medicine 90 days with drug withdrawal January after, respectively get 1/2 dog at random, after yellow Jackets anesthesia; Put to death respectively, become celestial as system, each internal organs situation of change is substantially observed by the comprehensive system of naked eyes; The tissue that notes abnormalities, emphasis carries out histological examination.Getting brain, the heart, liver, kidney, adrenal gland, testis, prostate, ovary, uterus takes by weighing weight in wet base, calculates organ coefficient.Above-mentioned internal organs and stomach, thymus gland and the epididymises etc. organized are through formaldehyde fixed, HE dyeing.Carry out histopathologic examination.
(3) the index review time: (1) normal value is measured: before the experiment beginning, carry out the detection of electrocardio, hematology, blood biochemical each item index.(2) experimental data is measured: carry out the mensuration that electrocardio, hematology and blood biochemical are learned each item index after the A. experimental session was taken medicine 45 days.B. 30 days (making invertibity observes) after experiment end and the drug withdrawal.Carry out that electrocardio, hematology, blood biochemical are learned, system becomes celestial and the detection of Histopathology index.
(4) experimental result
1. overview: the Beagle dog gives continuous 90 days of nutrient solution 8ml/kg of the present invention, 40ml/kg every day, do not observe dog take in after the nutrient solution of the present invention due to any bad projection.Behavioral activity is also normal, does not see phenomenons such as nauseating, vomiting, hydrostomia, apocleisis, ingests every day normally, and excreta (urine, excrement) is also no abnormal.Body weight all has growth in various degree, compares with control group, does not have tangible difference, sees table 2.
2. Electrocardioscopy (ECG): experimental applications standard lead II; Promptly connect right upper extremity and left lower extremity and be second and lead (II), before the medication, each group (control group, small dose group, heavy dose of group) dog all detects ECG once; Respectively detected ECG once in 45 days and 90 days during the medication; 30 days last dogs make ECG again once after the drug withdrawal, and each wave band of dog ECG does not all have obvious change, and the S-T section all belongs to normal range (NR).
3. hematological indices: dog gives nutrient solution of the present invention (8ml/kg-40ml/kg) 45 days, 90 days continuous every day, and the hematological examination index all fluctuates in normal range (NR), finds to take the influence of nutrient solution of the present invention to Beagle dog hematology measured value.See table 3.But need point out the clotting time when taking in 45 days, it is bigger fluctuation to occur, and all the other are compared with control group in measuring, and difference is not obvious.
4. blood parameters: compare in range of normal value before and after self of dog blood parameters measured value and fluctuate, compare with control group and do not see and take the influence of nutrient solution of the present invention, see table 4 to blood parameters.
LD
50Measure: get 40 of body weight 19-21gICR mouse, male and female half and half are divided into 4 groups immediately, and 10 every group, mouse supplies drinking-water, fasting 16 hours, and nutrient solution dosage of the present invention is 8-40ml/kg, the agent distance is 10ml.The administration capacity is the 0.4ml/10g body weight, behind the gastric infusion, observes animal immediately and reports situations successively, and observe once every day, continuous 7 days, and write down toxicity day by day and report situations and the animal dead number, the result sees table 1.
5. system's detection with the Histopathology index that becomes celestial
(1) become celestial: the naked eyes system is observed, and each treated animal main organs size, color and luster, quality etc. are not all seen obvious change.
(2) organ weights and organ coefficient are taken nutrient solution of the present invention 90 days and drug withdrawal 30 days, dog organ weights and organ coefficient, and compare nothing with control group significantly different, see table 5,6.
(3) check pathological section:
A. after taking medicine 90 days, all microscopy tissues such as each internal organs section microscopy brain, the heart, liver,spleen,kidney except that heavy dose of group (40ml/kg) thyroid gland, are not all seen obviously unusual.Heavy dose of group has 1 dog the atrophy of locality folliculus to occur, and colloid disappears.
B. invertibity was observed after drug withdrawal January, and inspection thyroid gland in section border respectively has a dog thyroid gland that focal folliculus atrophy is arranged in 8ml/kg group and 40ml/kg group, the colloid minimizing.Equal Non Apparent Abnormality is organized in all the other border inspections such as brain, the heart, kidney.
(5) conclusion
Nutrient solution 8ml/kg of the present invention and 40ml/kg (be equivalent to clinical dosage 20 times and 100 times); Beagle dog continuous oral 90 days; To body weight, ingest, each item index such as sign observation, electrocardio, hematology, blood biochemical all do not have tangible influence, do not see tangible poisoning symptom.Histopathologic examination, the perusal that becomes celestial, each organs and tissues has not been found unusually.Internal organs are weighed and are compared indifference with organ coefficient with control group, and microscopy is observed except that thyroid gland, and each is organized the equal no abnormality seen of histotomy that dog is examined and changes.Nutrient solution 8ml/kg group of the present invention has 2 dogs, 40ml/kg group to have 1 dog thyroid gland to observe focal folliculus atrophy, and the glue look reduces.
Embodiment 1: the production method of nutrient solution of the present invention is following:
The optimum weight of nutrient solution of the present invention (part) proportioning is:
Gynostemma pentaphylla: 44; Quail: 32; Red date: 8; The fruit of Chinese wolfberry: 6; Hawthorn: 3; Stichopus japonicus: 3; Arillus longan: 4.
1, will sting participation quail enzymolysis earlier; Get 3 parts of stichopus japonicus and soak bloatedly, put into the enzymolysis pot for 32 parts with the quail of cleaning chopping then, adds an amount of water, and add a certain amount of papain, in 50-60 Celsius ℃ temperature, be incubated enzymolysis 7-8 hour, can obtain hydrolyzed protein liquid;
2, extract the active ingredient of other plant material again: get 3 parts of hawthorn, 4 parts of arillus longans, 6 parts of the fruits of Chinese wolfberry; 8 parts in date, 44 parts of Gynostemma pentaphyllas are put into multi-functional extraction pot after the cleaning; Carry out second extraction active ingredient according to the Chinese medicine preparation rule, 1.5 hours for the first time, 1 hour for the second time; Temperature is controlled at 80-95 ℃, can obtain the extract of above-mentioned plant material;
3, hydrolyzed protein liquid that obtains and plant material extract are got final product by common process filter just, mixing, constant volume, smart filter, can, sterilization, lamp inspection, packing.
Claims (3)
1. a nutrient solution is characterized in that adopting the animal-plant material of following weight portion to process: gynostemma pentaphylla: 35-52; Quail: 24-38; Red date: 6-10; The fruit of Chinese wolfberry: 4-7; Hawthorn: 3-5; Stichopus japonicus: 2-4; Arillus longan: 2-5;
The production technology that above-mentioned nutrient solution adopts is: (1) will be stung at present and participate in the quail enzymolysis; Get stichopus japonicus and soak bloatedly, put into the enzymolysis pot with the quail of cleaning chopping then, adds an amount of water, and add a certain amount of papain, in 40-60 ℃ of temperature, be incubated enzymolysis 6-8 hour, can obtain hydrolyzed protein liquid;
(2) extract the active ingredient of other plant material again: get hawthorn, arillus longan, the fruit of Chinese wolfberry; Red date, gynostemma pentaphylla is put into multi-functional extraction pot after the cleaning; Carry out second extraction active ingredient according to the Chinese medicine preparation rule, 1.5 hours for the first time, 1 hour for the second time; Temperature is controlled at 60-100 ℃, can obtain the extract of above-mentioned plant material;
(3) hydrolyzed protein liquid that obtains and plant material extract are got final product by common process filter just, mixing, constant volume, smart filter, can, sterilization, lamp inspection, packing.
2. a kind of nutrient solution according to claim 1 is characterized in that adopting the animal-plant material of following component to process: gynostemma pentaphylla: 40-48; Quail: 27-35; Red date: 7-9; The fruit of Chinese wolfberry: 4-7; Hawthorn: 3-5; Stichopus japonicus: 3-4; Arillus longan: 2-4.
3. a kind of nutrient solution according to claim 1 is characterized in that adopting the animal-plant material of following component to process: gynostemma pentaphylla: 44; Quail: 32; Red date: 8; The fruit of Chinese wolfberry: 6; Hawthorn: 3; Stichopus japonicus: 3; Arillus longan: 4.
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|---|---|---|---|
| CN200710156348XA CN101194715B (en) | 2007-10-24 | 2007-10-24 | Strong nutrient solution |
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| CN200710156348XA CN101194715B (en) | 2007-10-24 | 2007-10-24 | Strong nutrient solution |
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| CN101194715A CN101194715A (en) | 2008-06-11 |
| CN101194715B true CN101194715B (en) | 2012-07-04 |
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| CN200710156348XA Active CN101194715B (en) | 2007-10-24 | 2007-10-24 | Strong nutrient solution |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1156004A (en) * | 1995-12-09 | 1997-08-06 | 史雄飞 | Drink and producing method |
| CN1494588A (en) * | 2001-03-08 | 2004-05-05 | Ĭ��ר������˾ | Use of protein histidine phosphatase |
| CN1860899A (en) * | 2006-06-13 | 2006-11-15 | 计建科 | Selenium-riched tea with fruit of Chinese wolfberry, and its prepn. art |
-
2007
- 2007-10-24 CN CN200710156348XA patent/CN101194715B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1156004A (en) * | 1995-12-09 | 1997-08-06 | 史雄飞 | Drink and producing method |
| CN1494588A (en) * | 2001-03-08 | 2004-05-05 | Ĭ��ר������˾ | Use of protein histidine phosphatase |
| CN1860899A (en) * | 2006-06-13 | 2006-11-15 | 计建科 | Selenium-riched tea with fruit of Chinese wolfberry, and its prepn. art |
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| CN101194715A (en) | 2008-06-11 |
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