CN101091789B - Application of ocean collagen peptide in preparing medicine or foodstuff for adjusting immunity - Google Patents

Application of ocean collagen peptide in preparing medicine or foodstuff for adjusting immunity Download PDF

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CN101091789B
CN101091789B CN2006100868128A CN200610086812A CN101091789B CN 101091789 B CN101091789 B CN 101091789B CN 2006100868128 A CN2006100868128 A CN 2006100868128A CN 200610086812 A CN200610086812 A CN 200610086812A CN 101091789 B CN101091789 B CN 101091789B
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marine collagen
collagen peptide
marine
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food
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CN101091789A (en
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李勇
蔡木易
王军波
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Jilin Peptide Valley Bioengineering Co Ltd
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Abstract

The present invention relates to an application of marine collagen peptide in preparation of medicine or food with the function of regulating immunological function of human body. Said marine collagen peptide is obtained by hydrolyzing fishskin of marine fishes (such as dog salmon and tilapia mossambica, etc.), and said marine collagen peptide is a micromolecular peptide whose molecular weight is200-1000D. The above-mentioned marine collagen peptide has the regulation action for cellular immunological function and humoral immunological function.

Description

The purposes of marine collagen in preparation immunoregulation medicament or food
Technical field
The present invention relates to the new purposes of marine collagen, is the purposes of marine collagen in preparation immunoregulation medicament or food specifically.Belong to health care of food and field of medicaments.
Background technology
In recent years, immunologic hypofunction, comprise constitutional and Secondary cases immunodeficiency, shortage or impaired, be subjected to domestic and international physicians' extensive concern,, particularly infect because they itself reach the various complication of being brought out, often make being in a bad way of patient, be difficult to treatment, and can cause serious consequence, even threat to life.If, improve body's immunity by the exploitation health food, economical and practical, will bring very big prospect to the health-care industry of China.
Some peptide that the plant and animal proteolysis produces has the immunocompetence effect.Meisel etc. (1996) confirm to have different biological activities in the casein sequence, can obtain following four kinds of bioactive peptide behind enzymolysis: 1. opioid agonists opiates agonist.2. opiiod anregonists opioid antagonist.3. immunostinulants (immunopeptides) immunostimulant.4. mineral Carriers (Caseinphosphopeptided) metallic carrier.The kyrine L eu-Leu-Tyr that the beta-casein hydrolysis generates and the N-terminal Thr-Thy-Met-Pro-Leu-Phe of alpha-casein can promote in test the phagocytosis of people and sheep intraperitoneal macrophage.Berthoa etc. (1981), Parker etc. separate two kinds of bioactive peptide that obtain from human milk casein enzymolysis, these two kinds of peptide concentrations of Val-Gln-Pro-Ile-Pro-tyr and Gly-Leu-Phe just can activate the phagocytosis of peritoneal macrophages to sheep red blood cell (SRBC) when 0.1umol/L.Polypeptide in Cosre etc. (1992) report beta-casein pepsin-Chymotrypsin digestion product can promote the propagation of the sophisticated lymphocytic immature splenocyte of rat.The Toplink that Azuma etc. (1989) extract from ovalbumin promotes the growth of cell and synthesizing of DNA.Scholars such as Chen Dongliang use the isotopic tracing zoopery and have confirmed that also albumin polypeptide and nucleic acid are edible jointly, can promote the absorption of nucleotide and utilize the abundance that promotes immune organ nucleic acid, it is synthetic to be of value to remedying of the interior nucleic acid metabolism of immunocyte, improves cell immune function of human body.
Marine collagen is one group of molecular weight being produced through hydrolysis by the fish skin of Oncorhynchi or tilapia small-molecular peptides at 200~1000D.Have easy absorption, absorb fast, low viscosity, characteristics such as good water solubility.There are some researches show that marine collagen has the effect of blood lipid regulation effect, bone density improving.
Summary of the invention
The present invention is intended to by further research, and the purposes of marine collagen in preparation immunoregulation medicament or food is provided, and provides a kind of new way for improve body's immunity by diet.
Wherein said medicine is powder, granule, capsule, tablet, pill.
Wherein said food is starch-based product, milk and milk products, bean product, baking goods.
Specific embodiments
Below illustrate marine collagen of the present invention at the action effect aspect the immunomodulating by zoopery.
1. material and method
1.1 given the test agent: marine collagen is prepared by Beijing crapulous syncope Hai Shi Bioisystech Co., Ltd.
1.2 laboratory animal: 192 of the female ICR mices of cleaning level, in 6~8 ages in week, 18~22 grams are provided by Department Of Medicine, Peking University's Experimental Animal Center, and approval number is SCXK11-00-0004, and cleaning level Animal House is raised 25 ℃ of feeding environment temperature, humidity 60%RH.
1.3 test rating: mouse spleen and thymus are heavy; Antibody forming cell's (IgM-PFC/ spleen) measures, and adopts Jerne improvement slide method; Serum antibody titer adopts serum half hemolysis value HC50; Delayed allergy (DTH) adopts the pedal swelling method to measure; The mice spleen lymphocytes proliferation function that ConA stimulates adopts mtt assay; The detection of macrophage phagocytic function is adopted carbon clearance method and peritoneal macrophage to engulf chicken red blood cell (half intracorporal method) and is measured; The NK cytoactive is measured, and adopts lactic acid dehydrogenase (LDH) algoscopy.
1.4 experimental technique: animal is divided into solvent control group, low dose group, middle dosage group and high dose group, and 48 every group, every group is further divided into 4 subgroups, 12 of each subgroups.Marine collagen is made into basic, normal, high three concentration, be 0.22gl/kg, 0.45g/kg and 1.35g/kg body weight, be equivalent to 5,10 and 30 times of people's consumption every day (2.7g/d) respectively, each experimental mice is irritated stomach once every day, irritate the stomach amount and press the 0.1ml/10g weighing machine, continuous irrigation stomach 30 days carries out These parameters according to Ministry of Public Health " the health food function assessment assessment process and the method for inspection " when experiment finishes and detects.
2. result
2.1 marine collagen is to mouse immune organ weight's influence: the result shows, gives marine collagen 30 days, and absolute weight and the relative weight to mouse spleen and thymus produces obviously influence.
2.2 marine collagen is to the mouse cell Immune Effects: by in the table 1 as seen, with matched group relatively, 0.45 and 1.35g/kgbw group mice pedal swelling degree significantly increase (P<0.05); Table 2 shows that each dosage group mouse lymphocyte propagation is significantly higher than matched group (P<0.05).The above results shows that marine collagen has tangible potentiation to the T lymphopoiesis of tardy paraphilia reaction (DTH) in the mouse cell immunologic function and ConA stimulation.
Table 1. marine collagen is to the influence of the tardy paraphilia reaction of mice (DTH)
Figure GSB00000129761200031
Figure GSB00000129761200032
*: significance is arranged, P<0.05 with matched group (0g/kgBW) comparing difference
The influence that the mouse T lymphocyte that table 2. marine collagen stimulates ConA is bred (DTH)
Figure GSB00000129761200034
*: significance is arranged, P<0.05 with matched group (0g/kgBW) comparing difference
2.3 marine collagen is to the influence of mouse humoral immune function: by in the table 3 as seen, with matched group relatively, 0.45 and 1.35g/kgbw group mouse antibodies cellulation quantity significantly increase (P<0.05); Each dosage group mice half hemolysis value of table 4 demonstration is compared with matched group and is not seen significant change (P>0.05).The above results shows that marine collagen can obviously improve the quantity of mouse antibodies cellulation, to the not obviously influence of mice serum antibody titer; The results suggest marine collagen has enhanced effect to the mouse humoral immune function.
Table 3. marine collagen is to the influence of mouse antibodies cellulation (IgM-PFC)
Figure GSB00000129761200035
Figure GSB00000129761200036
*: significance is arranged, P<0.05 with matched group (0g/kgBW) comparing difference
Table 4. marine collagen is to mice half hemolysis value (HC 50) influence
Figure GSB00000129761200037
Figure GSB00000129761200038
2.4 marine collagen is to the influence of macrophage phagocytosis of mice: by in the table 5 as seen, with matched group relatively, 0.45 and 1.35g/kgbw group mice carbon clearance index significantly increase (P<0.05); Each dosage group Turnover of Mouse Peritoneal Macrophages of table 6 demonstration is engulfed the phagocytic rate (PP) of chicken red blood cell and is compared all significantly raise (P<0.05) with matched group with phagocytic index (PI).The above results shows that marine collagen can obviously improve the quantity of mouse antibodies cellulation, to the not obviously influence of mice serum antibody titer; The results suggest marine collagen has enhanced effect to the mouse humoral immune function.
Table 5. marine collagen is to the influence of mice carbon clearance ability
Figure GSB00000129761200041
Figure GSB00000129761200042
*: significance is arranged, P<0.05 with matched group (0g/kgBw) comparing difference
Table 6. marine collagen is to the influence of mouse macrophage phagocytic rate (PP) and phagocytic index (PI)
*: significance is arranged, P<0.05 with matched group (0g/kg/BW) comparing difference
2.5 marine collagen is to the influence of mice NKT (NK) cytoactive: the result shows, with matched group relatively, give marine collagen 30 days and produce obviously influence NK cells in mice is active.
3. conclusion
Its mouse oral gave marine collagen after 30 days, and its testing result shows that the T lymphopoiesis that tardy paraphilia reaction (DTH) and ConA stimulate in the marine collagen pair cell immunologic function has tangible potentiation; Antibody-producting cell quantity in the humoral immune function had tangible rising effect; Has obvious facilitation to macrophage carbon clearance ability with to the phagocytic activity of chicken red blood cell; To sum up the result shows that marine collagen has immunoregulation effect.

Claims (3)

1. the purposes of marine collagen in preparation immunoregulation medicament or food, described marine collagen are one group of molecular weight producing through hydrolysis with the fish skin of Oncorhynchi or tilapia small-molecular peptides at 200-1000D.
2. by the described purposes of claim 1, wherein said medicine is powder, granule, capsule, tablet, pill.
3. by the described purposes of claim 1, wherein said food is starch-based product, milk and milk products, bean product, baking goods.
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CN102077942B (en) * 2010-09-20 2013-04-24 王强 Marine special dietary food special for patient with bone disease
CN102140467B (en) * 2010-12-30 2013-05-15 苏州吉玛基因股份有限公司 Human miR-365 antisense nucleic acid and application thereof
CN102356899B (en) * 2011-09-07 2012-11-28 中国食品发酵工业研究院 Natural fruit and vegetable ferment beverage, and preparation method thereof
CN106722999B (en) * 2016-12-31 2020-08-11 刘芙伽 Collagen peptide preparation method, low-sugar collagen beauty cake containing collagen peptide preparation method and preparation method
CN109350731A (en) * 2018-12-18 2019-02-19 汕尾市维明生物科技股份有限公司 A kind of Rofe fish protein peptide is preparing the application in immune formulation
CN111227259A (en) * 2020-03-10 2020-06-05 张家界(中国)金驰大鲵生物科技有限公司 Marine fish oligopeptide oyster oral liquid and preparation method thereof
CN117653562A (en) * 2023-11-27 2024-03-08 广州亚丽化妆品有限公司 Collagen peptide composition with anti-aging and immunity improving effects and preparation method thereof

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