CN101085994A - Ferment method of producing fuel ethanol using stalk material - Google Patents
Ferment method of producing fuel ethanol using stalk material Download PDFInfo
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- CN101085994A CN101085994A CNA2006100179040A CN200610017904A CN101085994A CN 101085994 A CN101085994 A CN 101085994A CN A2006100179040 A CNA2006100179040 A CN A2006100179040A CN 200610017904 A CN200610017904 A CN 200610017904A CN 101085994 A CN101085994 A CN 101085994A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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Abstract
The invention relates to a method for preparing fuel ethanol by taking straw as raw material. It comprises following steps: (1) selecting crop straw as raw material; (2) disintegrating: disintegrating straw by using hammer mill, sifting and getting straw powder of 20- 40 order; (3) pre- treating: lignin degrading raw material by way of culturing white-rot fungi in solid culture medium and liquid enzyme; (4) saccharifying two enzymes, saccharifying straw by using cellulase and xylanase at 45 Deg. C, pH 4.5, 150 rmp and ratio between liquid and solid 15, getting reduced sugar; (5) fermenting; (6) distilling. The fuel ethanol productivity is 16.7% in this invention, that is 16.7 ton waterless ethanol with every 100 ton straw, the production efficiency is high and economic benefit and social benefit is pretty good. Compared with current process, this invention is characterized in that the fermentation is thorough and the fuel ethanol production cost is low.
Description
Technical field
The present invention relates to a kind of fermentation process of producing alcohol fuel, relate in particular to a kind of fermentation process of producing fuel ethanol using stalk material.
Background technology
China is a large agricultural country, the vegetable fibre resource is very abundant, only the crop stalk annual output just reaches 700,000,000 tons, the present mode of utilizing mainly is that countryside kitchen work is used, part is the field also, makes energy usefulness by gasification, curing on a small quantity, utilizes level low, serious waste of resources, the environmental pollution that causes is serious.Have serious crisis and cause under the background of serious environmental pollution at traditional fossil energy such as coal, oil etc., alcohol fuel is renewable with it, non-environmental-pollution, use technology advantage such as reach a standard enjoys government and society to pay attention in motor vehicle.
Summary of the invention
The object of the present invention is to provide a kind of fermentation process of producing fuel ethanol using stalk material,, reduce the production of fuel ethanol cost greatly to effectively utilize stalk class resource.
To achieve these goals, technical program of the present invention lies in adopting a kind of fermentation process of producing fuel ethanol using stalk material, may further comprise the steps:
(1) chooses raw material: agricultural crop straw;
(2) pulverize: 20-40 order straw powder is pulverized, is screened into to the stalk straw through beater grinder;
(3) pre-treatment: raw material carries out lignin degradation through white-rot fungi under solid-state cultivation and liquid thick enzyme mode handles;
(4) double enzymes saccharification: adopting cellulase and zytase is under 15 the condition stalk to be carried out saccharification at 45 ℃, pH4.5,150rpm, liquid-solid ratio, obtains reducing sugar;
(5) fermentation: stalk saccharification liquid is carried out pentose to employing pachysolen tannophilus (Pachysolen tannophilus) As2.1585 and hexose is total to producing fuel ethyl alcohol by ferment, wherein fermentation system is configured to: add by following material and consumption thereof in stalk saccharification liquid, yeast decoction 0.2--0.4g/L, peptone 0.4--0.6g/L, urea 0.01--0.03g/L, Secondary ammonium phosphate 0.01--0.015g/L, polyvinyl alcohol is 0.005g/400mL-0.015g/400mL, tween 80 is 0.05ml/400mL--0.15g/400mL, L-glutaminate is 0.20g/400mL--0.26g/400mL, and adjusting medium pH is 5.0～5.5; Fermentation condition wherein: with substratum by 30% the liquid amount fermenting container of packing into, 121 ℃ of sterilization 30min, standby, at room temperature pachysolen tannophilus P-01 is inserted in the stalk saccharification liquid after the adjusted by 10% amount, under 30 ℃, carried out little aerobic fermentation 72 hours;
(6) distillation.
Agricultural crop straw in the step (1) can be any or its arbitrary combination in wheat stalk, maize straw, the straw.
Distillation in the step (6) can be adopted tower distillation or pillar distillation.
The bacterial classification that fermentation in the method for the present invention is adopted: pachysolen tannophilus (Pachysolentannophilus) As2.1585, Chinese Academy of Sciences's microbial strains preservation center preservation bacterial classification, this bacterial classification has significantly different with traditional ethanol fermentation bacterial strain, traditional ethanol fermentation bacterial strain only can transform hexoses such as glucose and form ethanol, form ethanol and pentoses such as wood sugar can not be transformed, this bacterial strain can transform pentose and form ethanol, and can simultaneously hexose and pentose be transformed formation ethanol.Because contain hexose about 70% and about 30% pentose in the stalk saccharification liquid, utilizing traditional ethanol fermentation bacterial strain to transform stalk, to form the alcoholic acid ratio just low, and to form alcoholic acid ratio higher and this bacterial strain transforms stalk.Adopt the special bacterium culture medium that is equipped with, make every gram mixing sugar to transform and form 0.36-0.39 gram dehydrated alcohol, improved the transformation efficiency of mixing sugar greatly, adopt the alcohol fuel productive rate of fermentation process production of the present invention to reach 16.7%, be that per 100 tons of stalks can be produced 16.7 tons of dehydrated alcohols, the production efficiency height has good economic worth and social value, is easy to apply.
The present invention compares with existing production alcoholic acid fermentation process: bacterial strain of the present invention available total sugar-fermenting under specific fermentation system is thorough, and the alcohol fuel cost is low.
Specific as follows:
| Technology | Transform and form alcoholic acid sugar | Total reducing sugar changes into alcoholic acid ratio (%) | Product ton cost (unit) |
| The present invention | Pentose and hexose | 38-40 | 3100 |
| Tradition ethanol fermentation technology (as Nanyang distillery yeast 1308) | Hexose | 32-35 | 3400 |
| Adopt pentose fermentation bacterial strain (as pichia) | Pentose | 12-15 | 3600 |
Embodiment
Embodiment 1
The fermentation process of the producing fuel ethanol using stalk material in the present embodiment may further comprise the steps:
(1) chooses raw material: agricultural crop straw, wheat stalk, maize straw, straw;
(2) pulverize: 20-40 order straw powder is pulverized, is screened into to the stalk straw through beater grinder;
(3) pre-treatment: raw material carries out lignin degradation through white-rot fungi under solid-state cultivation and liquid thick enzyme mode handles;
(4) double enzymes saccharification: adopting cellulase and zytase is under 15 the condition stalk to be carried out saccharification at 45 ℃, pH4.5,150rpm, liquid-solid ratio, obtains reducing sugar;
(5) fermentation: stalk saccharification liquid is carried out pentose to employing pachysolen tannophilus (Pachysolen tannophilus) As2.1585 and hexose is total to producing fuel ethyl alcohol by ferment, wherein fermentation system is configured to: add by following material and consumption thereof in stalk saccharification liquid, yeast decoction 0.2g/L, peptone 0.4g/L, urea 0.01g/L, Secondary ammonium phosphate 0.01g/L, polyvinyl alcohol is 0.005g/400mL, tween 80 is 0.05ml/400mL, L-glutaminate is 0.20g/400mL, and adjusting medium pH is 5.0; Fermentation condition wherein: with substratum by 30% the liquid amount fermenting container of packing into, 121 ℃ of sterilization 30min, standby, at room temperature pachysolen tannophilus P-01 is inserted in the stalk saccharification liquid after the adjusted by 10% amount, under 30 ℃, carried out little aerobic fermentation 72 hours;
(6) distillation can be adopted the distillation of tower distillation or pillar.
Embodiment 2
The fermentation process of the producing fuel ethanol using stalk material in the present embodiment may further comprise the steps:
(1) chooses raw material: agricultural crop straw, wheat stalk, maize straw, straw;
(2) pulverize: 20-40 order straw powder is pulverized, is screened into to the stalk straw through beater grinder;
(3) pre-treatment: raw material carries out lignin degradation through white-rot fungi under solid-state cultivation and liquid thick enzyme mode handles;
(4) double enzymes saccharification: adopting cellulase and zytase is under 15 the condition stalk to be carried out saccharification at 45 ℃, pH4.5,150rpm, liquid-solid ratio, obtains reducing sugar;
(5) fermentation: stalk saccharification liquid is carried out pentose to employing pachysolen tannophilus (Pachysolen tannophilus) As2.1585 and hexose is total to producing fuel ethyl alcohol by ferment, wherein fermentation system is configured to: add by following material and consumption thereof in stalk saccharification liquid, yeast decoction 0.3g/L, peptone 0.5g/L, urea 0.02g/L, Secondary ammonium phosphate 0.02g/L, polyvinyl alcohol is 0.01g/400mL, tween 80 is 0.1ml/400mL, L-glutaminate is 0.25g/400mL, and adjusting medium pH is 5.0; Fermentation condition wherein: with substratum by 30% the liquid amount fermenting container of packing into, 121 ℃ of sterilization 30min, standby, at room temperature pachysolen tannophilus P-01 is inserted in the stalk saccharification liquid after the adjusted by 10% amount, under 30 ℃, carried out little aerobic fermentation 72 hours;
(6) distillation can be adopted the distillation of tower distillation or pillar.
Embodiment 3
The fermentation process of the producing fuel ethanol using stalk material in the present embodiment may further comprise the steps:
(1) chooses raw material: agricultural crop straw, wheat stalk, maize straw, straw;
(2) pulverize: 20-40 order straw powder is pulverized, is screened into to the stalk straw through beater grinder;
(3) pre-treatment: raw material carries out lignin degradation through white-rot fungi under solid-state cultivation and liquid thick enzyme mode handles;
(4) double enzymes saccharification: adopting cellulase and zytase is under 15 the condition stalk to be carried out saccharification at 45 ℃, pH4.5,150rpm, liquid-solid ratio, obtains reducing sugar;
(5) fermentation: stalk saccharification liquid is carried out pentose to employing pachysolen tannophilus (Pachysolen tannophilus) As2.1585 and hexose is total to producing fuel ethyl alcohol by ferment, wherein fermentation system is configured to: add by following material and consumption thereof in stalk saccharification liquid, yeast decoction 0.6g/L, peptone 0.6g/L, urea 0.05g/L, Secondary ammonium phosphate 0.05g/L, polyvinyl alcohol is 0.015g/400mL, tween 80 is 0.15ml/400mL, L-glutaminate is 0.26g/400mL, and adjusting medium pH is 5.0; Fermentation condition wherein: with substratum by 30% the liquid amount fermenting container of packing into, 121 ℃ of sterilization 30min, standby, at room temperature pachysolen tannophilus P-01 is inserted in the stalk saccharification liquid after the adjusted by 10% amount, under 30 ℃, carried out little aerobic fermentation 72 hours;
(6) distillation can be adopted the distillation of tower distillation or pillar.
It should be noted last that: above embodiment is the unrestricted technical scheme of the present invention in order to explanation only, although the present invention is had been described in detail with reference to the foregoing description, those of ordinary skill in the art is to be understood that: still can make amendment or be equal to replacement the present invention, and not breaking away from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (3)
1, a kind of fermentation process of producing fuel ethanol using stalk material is characterized in that: may further comprise the steps:
(1) chooses raw material: agricultural crop straw;
(2) pulverize: 20-40 order straw powder is pulverized, is screened into to the stalk straw through beater grinder;
(3) pre-treatment: raw material carries out lignin degradation through white-rot fungi under solid-state cultivation and liquid thick enzyme mode handles;
(4) double enzymes saccharification: adopting cellulase and zytase is under 15 the condition stalk to be carried out saccharification at 45 ℃, pH4.5,150rpm, liquid-solid ratio, obtains reducing sugar;
(5) fermentation: stalk saccharification liquid is carried out pentose to employing pachysolen tannophilus (Pachysolen tannophilus) As2.1585 and hexose is total to producing fuel ethyl alcohol by ferment, wherein fermentation system is configured to: add by following material and consumption thereof in stalk saccharification liquid, yeast decoction 0.2--0.4g/L, peptone 0.4--0.6g/L, urea 0.01--0.03g/L, Secondary ammonium phosphate 0.01--0.015g/L, polyvinyl alcohol is 0.005g/400mL--0.015g/400mL, tween 80 is 0.05ml/400mL--0.15g/400mL, L-glutaminate is 0.20g/400mL--0.26g/400mL, and adjusting medium pH is 5.0～5.5; Fermentation condition wherein: with substratum by 30% the liquid amount fermenting container of packing into, 121 ℃ of sterilization 30min, standby, at room temperature pachysolen tannophilus P-01 is inserted in the stalk saccharification liquid after the adjusted by 10% amount, under 30 ℃, carried out little aerobic fermentation 72 hours;
(6) distillation.
2, the fermentation process of producing fuel ethanol using stalk material according to claim 1 is characterized in that: the agricultural crop straw in the step (1) can be any or its arbitrary combination in wheat stalk, maize straw, the straw.
3, the fermentation process of producing fuel ethanol using stalk material according to claim 1 is characterized in that: the distillation in the step (6) can be adopted tower distillation or pillar distillation.
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| CNA2006100179040A CN101085994A (en) | 2006-06-06 | 2006-06-06 | Ferment method of producing fuel ethanol using stalk material |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102146211A (en) * | 2011-01-04 | 2011-08-10 | 聊城大学 | Cellulose composition capable of improving saccharifying efficiency and sugar processing method |
| CN102181510A (en) * | 2011-03-07 | 2011-09-14 | 合肥工业大学 | Technological process for producing ethanol through steam explosion and fermentation of trunks and branches of poplar trees |
| CN102191281A (en) * | 2011-03-15 | 2011-09-21 | 黑龙江省轻工科学研究院 | Method for manufacturing fuel alcohol through fermenting crop straw |
| CN102703519A (en) * | 2012-06-26 | 2012-10-03 | 太仓市周氏化学品有限公司 | Method for fermenting and producing cellulosic ethanol by means of pachysolen tannophilus |
| CN102978259A (en) * | 2012-12-05 | 2013-03-20 | 中国农业科学院农产品加工研究所 | Method for improving reducing sugar content of straws |
| CN101831337B (en) * | 2009-03-12 | 2013-03-20 | 尚征 | Process technique for producing crop straw fuel |
| CN104523485A (en) * | 2014-12-12 | 2015-04-22 | 柳州市京阳节能科技研发有限公司 | Technology for refining environment-friendly detergent by natural straws, vanilla and sensitive plants |
| CN104561129A (en) * | 2014-12-31 | 2015-04-29 | 新乡医学院 | Enzymolysis saccharification processing method of straw |
| CN106472818A (en) * | 2016-09-23 | 2017-03-08 | 河南农业大学 | A kind of utilization Coptotermes formosanus Shtrari. body is homogenized the method that steam explosion prepares high glycogen material |
| CN106834359A (en) * | 2009-02-11 | 2017-06-13 | 希乐克公司 | Processing biomass |
| CN107227319A (en) * | 2009-12-21 | 2017-10-03 | 诺维信公司 | Method for producing tunning from lignocellulose-containing materials |
| CN108728500A (en) * | 2018-06-26 | 2018-11-02 | 深圳崀途科技有限公司 | A kind of technique that alcohol fuel is prepared using stalk |
| CN110066833A (en) * | 2019-04-29 | 2019-07-30 | 江苏一鸣生物科技有限公司 | A method of ethyl alcohol is prepared using combinatorial enzymatic catalysis degrading straw |
| CN111334443A (en) * | 2020-01-09 | 2020-06-26 | 河北民族师范学院 | Saccharomyces cerevisiae strain tolerant to vanillic acid, p-hydroxybenzoic acid and syringic acid and application thereof |
| CN113403347A (en) * | 2021-08-05 | 2021-09-17 | 苏州迈博汇生物科技有限公司 | Alcohol production process with high alcohol yield |
-
2006
- 2006-06-06 CN CNA2006100179040A patent/CN101085994A/en active Pending
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106834359A (en) * | 2009-02-11 | 2017-06-13 | 希乐克公司 | Processing biomass |
| CN101831337B (en) * | 2009-03-12 | 2013-03-20 | 尚征 | Process technique for producing crop straw fuel |
| CN107227319A (en) * | 2009-12-21 | 2017-10-03 | 诺维信公司 | Method for producing tunning from lignocellulose-containing materials |
| CN102146211B (en) * | 2011-01-04 | 2012-11-07 | 聊城大学 | Cellulose composition capable of improving saccharifying efficiency and sugar processing method |
| CN102146211A (en) * | 2011-01-04 | 2011-08-10 | 聊城大学 | Cellulose composition capable of improving saccharifying efficiency and sugar processing method |
| CN102181510A (en) * | 2011-03-07 | 2011-09-14 | 合肥工业大学 | Technological process for producing ethanol through steam explosion and fermentation of trunks and branches of poplar trees |
| CN102181510B (en) * | 2011-03-07 | 2013-04-17 | 合肥工业大学 | Technological process for producing ethanol through steam explosion and fermentation of trunks and branches of poplar trees |
| CN102191281A (en) * | 2011-03-15 | 2011-09-21 | 黑龙江省轻工科学研究院 | Method for manufacturing fuel alcohol through fermenting crop straw |
| CN102703519A (en) * | 2012-06-26 | 2012-10-03 | 太仓市周氏化学品有限公司 | Method for fermenting and producing cellulosic ethanol by means of pachysolen tannophilus |
| CN102978259A (en) * | 2012-12-05 | 2013-03-20 | 中国农业科学院农产品加工研究所 | Method for improving reducing sugar content of straws |
| CN104523485A (en) * | 2014-12-12 | 2015-04-22 | 柳州市京阳节能科技研发有限公司 | Technology for refining environment-friendly detergent by natural straws, vanilla and sensitive plants |
| CN104561129A (en) * | 2014-12-31 | 2015-04-29 | 新乡医学院 | Enzymolysis saccharification processing method of straw |
| CN106472818A (en) * | 2016-09-23 | 2017-03-08 | 河南农业大学 | A kind of utilization Coptotermes formosanus Shtrari. body is homogenized the method that steam explosion prepares high glycogen material |
| CN106472818B (en) * | 2016-09-23 | 2019-08-20 | 河南农业大学 | A method of high glycogen material is prepared using termite body homogenate steam blasting |
| CN108728500A (en) * | 2018-06-26 | 2018-11-02 | 深圳崀途科技有限公司 | A kind of technique that alcohol fuel is prepared using stalk |
| CN110066833A (en) * | 2019-04-29 | 2019-07-30 | 江苏一鸣生物科技有限公司 | A method of ethyl alcohol is prepared using combinatorial enzymatic catalysis degrading straw |
| CN111334443A (en) * | 2020-01-09 | 2020-06-26 | 河北民族师范学院 | Saccharomyces cerevisiae strain tolerant to vanillic acid, p-hydroxybenzoic acid and syringic acid and application thereof |
| CN111334443B (en) * | 2020-01-09 | 2022-09-23 | 河北民族师范学院 | Saccharomyces cerevisiae strain tolerant to vanillic acid, p-hydroxybenzoic acid and syringic acid and application thereof |
| CN113403347A (en) * | 2021-08-05 | 2021-09-17 | 苏州迈博汇生物科技有限公司 | Alcohol production process with high alcohol yield |
| CN113403347B (en) * | 2021-08-05 | 2023-08-15 | 苏州迈博汇生物科技有限公司 | Alcohol production process with high alcohol yield |
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