CN101034065A - 乳粉蛋白质含量快速检测试剂盒及其制备方法 - Google Patents
乳粉蛋白质含量快速检测试剂盒及其制备方法 Download PDFInfo
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- CN101034065A CN101034065A CN 200610056997 CN200610056997A CN101034065A CN 101034065 A CN101034065 A CN 101034065A CN 200610056997 CN200610056997 CN 200610056997 CN 200610056997 A CN200610056997 A CN 200610056997A CN 101034065 A CN101034065 A CN 101034065A
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- 238000012360 testing method Methods 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 36
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 32
- OHJMTUPIZMNBFR-UHFFFAOYSA-N biuret Chemical compound NC(=O)NC(N)=O OHJMTUPIZMNBFR-UHFFFAOYSA-N 0.000 claims abstract description 63
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 40
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 36
- 239000012460 protein solution Substances 0.000 claims abstract description 30
- 238000001514 detection method Methods 0.000 claims abstract description 24
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- 239000000523 sample Substances 0.000 description 53
- 239000000047 product Substances 0.000 description 21
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- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 16
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- 238000002835 absorbance Methods 0.000 description 6
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- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
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Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
样品编号 | A | B | C | D | E |
蛋白质含量(%) | 20 | 25 | 15-20 | 10-15 | 20 |
样品编号 | A | B | C | D | E |
蛋白质含量(%) | 20 | 20-30 | 15-20 | 10-15 | 20 |
样品编号 | A | B | C | D | E |
蛋白质含量(%) | 19.67 | 25.08 | 17.92 | 12.70 | 19.20 |
样品编号 | A | B | C | D | E |
蛋白质含量(%) | 20.2 | 24.0 | 17.2 | 13.2 | 19.7 |
样品编号 | 蛋白质含量(%) | ||||
产品标称值 | 实施例3 | 实施例4 | 对比例1 | 对比例2 | |
A | ≥18.5 | 20 | 20 | 19.67 | 20.2 |
B | 24 | 25 | 20-30 | 25.08 | 24.0 |
C | 17 | 15-20 | 15-20 | 17.92 | 17.2 |
D | 12.6 | 10-15 | 10-15 | 12.7 | 13.2 |
E | ≥18.5 | 20 | 20 | 19.2 | 19.7 |
方法名称 | 试剂盒法 | 凯氏定氮法 | 常规双缩脲法 |
仪器设备 | 少 | 多 | 较多 |
试剂 | 一种 | 多 | 较多 |
操作步骤 | 简单 | 复杂繁琐 | 较繁琐 |
所需时间 | 5-10分钟 | 3.5-4.5小时 | 1.5-2小时 |
人员要求 | 无特殊要求 | 专业人员 | 专业人员 |
场地要求 | 无特殊要求 | 实验室中 | 实验室中 |
结果 | 半定量 | 准确 | 较准确 |
现场检测 | 适合 | 不适合 | 不适合 |
Claims (10)
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CN200610056997A CN100593114C (zh) | 2006-03-07 | 2006-03-07 | 乳粉蛋白质含量快速检测试剂盒及其制备方法 |
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CN200610056997A CN100593114C (zh) | 2006-03-07 | 2006-03-07 | 乳粉蛋白质含量快速检测试剂盒及其制备方法 |
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CN101034065A true CN101034065A (zh) | 2007-09-12 |
CN100593114C CN100593114C (zh) | 2010-03-03 |
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN102288597A (zh) * | 2011-05-12 | 2011-12-21 | 周逸明 | 一种快速简便的多肽残留检测方法 |
CN102879538A (zh) * | 2012-08-26 | 2013-01-16 | 大连理工大学 | 一种测定乳粉中蛋白质含量的方法 |
CN102944550A (zh) * | 2012-10-15 | 2013-02-27 | 上海谱尼测试技术有限公司 | 面粉中过氧化钙的检测试剂盒及过氧化钙检测方法 |
CN103278469A (zh) * | 2013-05-24 | 2013-09-04 | 宁波美康生物科技股份有限公司 | 一种总蛋白检测试剂 |
CN104155292A (zh) * | 2014-06-19 | 2014-11-19 | 东力(南通)化工有限公司 | 一种米屈肼中间体3-(2,2,2-三甲基肼)丙酸甲酯甲基硫酸盐含量的检测方法 |
CN105486682A (zh) * | 2015-12-28 | 2016-04-13 | 东北农业大学 | 一种蛋白质快速检测试剂盒及其检测方法和应用 |
CN107179316A (zh) * | 2017-07-19 | 2017-09-19 | 山东大学 | 一种海参口服液快速鉴定试剂盒 |
CN108489972A (zh) * | 2018-03-02 | 2018-09-04 | 成都虹波钼业有限责任公司 | 一种快速测定溶液中低含量钼的方法 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2021089707A1 (en) * | 2019-11-07 | 2021-05-14 | Nanonord A/S | A method of and a system for determining protein concentration in a selected material by nuclear magnetic resonance relaxometry |
-
2006
- 2006-03-07 CN CN200610056997A patent/CN100593114C/zh not_active Expired - Fee Related
Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102288597A (zh) * | 2011-05-12 | 2011-12-21 | 周逸明 | 一种快速简便的多肽残留检测方法 |
CN102288597B (zh) * | 2011-05-12 | 2016-01-13 | 上海苏豪逸明制药有限公司 | 一种快速简便的多肽残留检测方法 |
CN102879538A (zh) * | 2012-08-26 | 2013-01-16 | 大连理工大学 | 一种测定乳粉中蛋白质含量的方法 |
CN102944550A (zh) * | 2012-10-15 | 2013-02-27 | 上海谱尼测试技术有限公司 | 面粉中过氧化钙的检测试剂盒及过氧化钙检测方法 |
CN102944550B (zh) * | 2012-10-15 | 2015-07-15 | 上海谱尼测试技术有限公司 | 面粉中过氧化钙的检测试剂盒及过氧化钙检测方法 |
CN103278469A (zh) * | 2013-05-24 | 2013-09-04 | 宁波美康生物科技股份有限公司 | 一种总蛋白检测试剂 |
CN103278469B (zh) * | 2013-05-24 | 2015-08-12 | 宁波美康生物科技股份有限公司 | 一种总蛋白检测试剂 |
CN104155292A (zh) * | 2014-06-19 | 2014-11-19 | 东力(南通)化工有限公司 | 一种米屈肼中间体3-(2,2,2-三甲基肼)丙酸甲酯甲基硫酸盐含量的检测方法 |
CN105486682A (zh) * | 2015-12-28 | 2016-04-13 | 东北农业大学 | 一种蛋白质快速检测试剂盒及其检测方法和应用 |
CN107179316A (zh) * | 2017-07-19 | 2017-09-19 | 山东大学 | 一种海参口服液快速鉴定试剂盒 |
CN108489972A (zh) * | 2018-03-02 | 2018-09-04 | 成都虹波钼业有限责任公司 | 一种快速测定溶液中低含量钼的方法 |
CN108489972B (zh) * | 2018-03-02 | 2021-03-02 | 成都虹波钼业有限责任公司 | 一种快速测定溶液中低含量钼的方法 |
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Denomination of invention: Reagent kit for quickly testing protein content of milk powder and method for making same Effective date of registration: 20180228 Granted publication date: 20100303 Pledgee: Huaxia Bank Beijing branch Wanliu Limited by Share Ltd. Pledgor: BEIJING WISDOM VENTA TECHNOLOGY CO.,CORP Registration number: 2018990000152 |
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Date of cancellation: 20191224 Granted publication date: 20100303 Pledgee: Huaxia Bank Beijing branch Wanliu Limited by Share Ltd. Pledgor: BEIJING WISDOM VENTA TECHNOLOGY CO.,CORP Registration number: 2018990000152 |
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