CN100587067C - Method for producing keratinase by liquid deep fermentation - Google Patents
Method for producing keratinase by liquid deep fermentation Download PDFInfo
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- CN100587067C CN100587067C CN200810060639A CN200810060639A CN100587067C CN 100587067 C CN100587067 C CN 100587067C CN 200810060639 A CN200810060639 A CN 200810060639A CN 200810060639 A CN200810060639 A CN 200810060639A CN 100587067 C CN100587067 C CN 100587067C
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- 239000007788 liquid Substances 0.000 title claims abstract description 25
- 238000000855 fermentation Methods 0.000 title claims abstract description 18
- 230000004151 fermentation Effects 0.000 title claims abstract description 18
- 108010059345 keratinase Proteins 0.000 title claims abstract description 13
- 238000004519 manufacturing process Methods 0.000 title abstract description 7
- 239000000843 powder Substances 0.000 claims abstract description 32
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 24
- 238000000034 method Methods 0.000 claims abstract description 22
- 239000001963 growth medium Substances 0.000 claims abstract description 17
- 229940041514 candida albicans extract Drugs 0.000 claims abstract description 11
- 239000012138 yeast extract Substances 0.000 claims abstract description 11
- 239000002609 medium Substances 0.000 claims description 24
- 230000003716 rejuvenation Effects 0.000 claims description 24
- 239000001888 Peptone Substances 0.000 claims description 23
- 108010080698 Peptones Proteins 0.000 claims description 23
- 235000019319 peptone Nutrition 0.000 claims description 23
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 23
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 17
- 239000000203 mixture Substances 0.000 claims description 17
- 238000011218 seed culture Methods 0.000 claims description 17
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 claims description 14
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 14
- 239000003292 glue Substances 0.000 claims description 9
- 229920001817 Agar Polymers 0.000 claims description 8
- 239000008272 agar Substances 0.000 claims description 8
- 238000011534 incubation Methods 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 8
- 241000611330 Chryseobacterium Species 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 102000011782 Keratins Human genes 0.000 abstract description 14
- 108010076876 Keratins Proteins 0.000 abstract description 14
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 abstract description 8
- 235000021240 caseins Nutrition 0.000 abstract description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 abstract description 6
- 239000008103 glucose Substances 0.000 abstract description 6
- 230000000050 nutritive effect Effects 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 241000589593 Chryseobacterium gleum Species 0.000 abstract 3
- 102000011632 Caseins Human genes 0.000 abstract 1
- 108010076119 Caseins Proteins 0.000 abstract 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract 1
- GEHJBWKLJVFKPS-UHFFFAOYSA-N bromochloroacetic acid Chemical compound OC(=O)C(Cl)Br GEHJBWKLJVFKPS-UHFFFAOYSA-N 0.000 abstract 1
- 229940021722 caseins Drugs 0.000 abstract 1
- 239000007857 degradation product Substances 0.000 abstract 1
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 159000000003 magnesium salts Chemical class 0.000 abstract 1
- 159000000000 sodium salts Chemical class 0.000 abstract 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 11
- 229920002472 Starch Polymers 0.000 description 7
- 229930006000 Sucrose Natural products 0.000 description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 7
- 239000005018 casein Substances 0.000 description 7
- 239000008107 starch Substances 0.000 description 7
- 235000019698 starch Nutrition 0.000 description 7
- 239000005720 sucrose Substances 0.000 description 7
- 239000013028 medium composition Substances 0.000 description 6
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 3
- 210000003746 feather Anatomy 0.000 description 3
- 230000007062 hydrolysis Effects 0.000 description 3
- 238000006460 hydrolysis reaction Methods 0.000 description 3
- 210000002268 wool Anatomy 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 102100037157 Keratin, type I cytoskeletal 40 Human genes 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 238000010564 aerobic fermentation Methods 0.000 description 2
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 101710200191 Feather keratin Proteins 0.000 description 1
- 101710183583 Keratin, type I cytoskeletal 40 Proteins 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000006180 nutrition needs Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a method for liquid submerged fermentation production of keratinase by utilization of Chryseobacterium Gleum, aiming at large-scale liquid submerged fermentation production ofthe keratinase. The Chrvseobacterium L99, CGMCC No.2295 pure strain separated by the laboratory is used as an initial strain, and liquid submerged fermentation production of the keratinase is realizedthrough slant cultivation, shaking seed cultivation and fermenter cultivation. A liquid fermentation culture medium disclosed in the invention respectively consists of tragantine, sugar, glucose, caseins, yeast powder, yeast extract paste, pepetone, keratins, magnesium salts, sylvin and sodium salts. The method is suitable for liquid submerged aerated culture of the Chryseobacterium Gleum, reasonably meets the nutritive materials required by different fermentation periods of the Chryseobacterium Gleum, and improves the activity of the keratinase in fermentation broth. The method can degrade the keratin which causes environmental pollution when the keratinase is produced simultaneously, and keratin degradation products obtained have high economic value.
Description
Technical field
The present invention relates to the microbial engineering field, particularly a kind of method of producing keratinase by liquid deep fermentation.
Background technology
M-Zyme can gentleness Keratin sulfate waste such as hydrolysis feather efficiently, produce feed, fertilizer.The feed saving energy, the cost that this method is produced is low, quality good, can be utilized by animal preferably.In containing keratic feed, the interpolation M-Zyme also can improve animal and absorb keratic, increases economic efficiency.Keratin sulfate can the selective hydrolysis wool, the Keratin sulfate of leather surface, is expected to replace traditional wool stripping squama and tanning process, realizes the environmental protection transformation of conventional industries.M-Zyme can improve makeup, the externally applied medicine permeability in skin, also all has a wide range of applications in industry such as medicine, makeup.The M-Zyme production technology of exploitation Cheap highly effective is protection environment and the inevitable requirement of increasing economic efficiency.
This will glue production that golden yellow bacillus liquid fermentation technology is applied to M-Zyme and still belong to the firstly at home, and result of study shows that this scheme is feasible.Sticking golden yellow bacillus is Chryseobacterium L99, is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.2295.
Summary of the invention
The objective of the invention is relation, a kind of method of producing keratinase by liquid deep fermentation is provided at nutritional needs in the liquid fermenting process and thalli growth.
The method of producing keratinase by liquid deep fermentation comprises the steps:
1) adopts the method that is coated with or rules to glue golden yellow bacillus and be inoculated on the slant medium, cultivated 18~36 hours, obtain the rejuvenation thalline in 18~37 ℃ of rejuvenation;
2) the rejuvenation thalline is inoculated in the liquid seed culture medium,, cultivated under 100~300rpm condition 18~36 hours, obtain ferment-seeded in 18~37 ℃;
3) ferment-seeded is pressed 5~15% of fermention medium volume and inserted in the fermentor tank, jar temperature 18~37 ℃, tank pressure 0.2~0.8kg/cm
2, stir speed (S.S.) 100~300rpm, air flow are 1: 0.3~2.0, fermented incubation time 18~60 hours.
Described sticking golden yellow bacillus is Chryseobacterium L99, is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.2295.
The composition of described slant medium is: contain 900~1000 milliliters in NaCl5~15 grams, yeast powder or yeast extract paste 5~10 grams, peptone 5~15 grams, agar powder 10~20 grams and water in per 1000 milliliters.
The composition of described seed culture medium is: contain 900~1000 milliliters in NaCl5~15 grams, yeast powder or yeast extract paste 5~10 grams, peptone 5~15 grams and water in per 1000 milliliters.
The composition of described fermention medium is: soluble-containing starch 0.1~100 gram, sucrose 0.1~100 gram, glucose 0.1~100 gram, casein 0.1~100 gram, yeast powder or yeast extract paste 0.1~100 gram, peptone 0.1~100 gram, Keratin sulfate 0.1~200 gram, K in per 1000 milliliters
2PO
40.1~10 grams, NaH
2PO
42H
2O1~10 grams, MgCl
26H
2900~1000 milliliters in O 0.1~5 gram and water.
The beneficial effect that the present invention compared with prior art has:
1) seed culture medium provides the thalline required nutritive substance of growing fast in a short time among the present invention, cultivates can carry out submerged fermentation in about 20 hours.
2) liquid submerged aerobic fermentation base among the present invention, the nutritive ingredient reasonable ratio can guarantee the growth of sticking golden yellow bacillus L99 and produce enzyme require.The substratum material is abundant, only needs once batching in the whole batch fermentation process, once sterilization get final product, have save time, the saving of labor, reduced again because the reinforced midway chance that polluting the bacterium of mixing.
3) adopt the deep liquid aerobic fermentation technology among the present invention to carry out batch fermentation, final enzyme work can reach 100-400U/ml.The enzyme activity unit definition: 8.0,50 ℃ of pH, the 200rpm per hour blue Keratin sulfate of hydrolysis make 595nm place absorbancy increase by 0.001 enzyme amount.
4) the present invention domesticly produces the M-Zyme zymotechnique to sticking golden yellow bacillus first and studies, and experimental study and the suitability for industrialized production later for this bacterium all have very big directive significance.
5) utilized in the fermenting process of the present invention the disadvantageous Keratin sulfate of environmental protection is substrate, cost is low, can solve environmental problem again when producing M-Zyme, and the Keratin sulfate after the degraded is used for industry such as feed can produce higher economic value.
Embodiment
Below in conjunction with specific embodiment the present invention is described in further detail.
Embodiment 1
1) adopts the method that is coated with or rules to glue golden yellow bacillus and be inoculated on the slant medium, cultivated 36 hours, obtain the rejuvenation thalline in 18 ℃ of rejuvenation; The composition of slant medium is: contain 980 milliliters in NaCl10 gram, yeast powder 5 grams, peptone 5 grams, agar powder 15 grams and water in per 1000 milliliters.
2) the rejuvenation thalline is inoculated in the liquid seed culture medium,, cultivated 24 hours under the 200rpm condition, obtain ferment-seeded in 18 ℃; The composition of seed culture medium is: 980 milliliters in NaCl10 in per 1000 milliliters gram, yeast powder 5 grams, peptone 5 grams and water.
3) ferment-seeded is pressed 5% of fermention medium volume and inserted in the fermentor tank, jar temperature 18 ℃, tank pressure 0.2kg/cm
2, stir speed (S.S.) 100rpm, air flow are 1: 0.3, fermented incubation time 60 hours.The fermention medium composition is: soluble-containing starch 0.1 gram, sucrose 0.1 gram, glucose 0.1 gram, casein 0.1 gram, yeast powder 0.1 gram, peptone 0.1 gram, feather meal Keratin sulfate 20 grams, K in per 1000 milliliters
2PO
42 grams, NaH
2PO
42H
2O 4 grams, MgCl
26H
2980 milliliters in O 0.1 gram and water.
Embodiment 2
1) adopts the method that is coated with or rules to glue golden yellow bacillus and be inoculated on the slant medium, cultivated 24 hours, obtain the rejuvenation thalline in 25 ℃ of rejuvenation; The composition of slant medium is: contain 980 milliliters in NaCl10 gram, yeast powder 5 grams, peptone 10 grams, agar powder 20 grams and water in per 1000 milliliters.
2) the rejuvenation thalline is inoculated in the liquid seed culture medium,, cultivated 18 hours under the 200rpm condition, obtain ferment-seeded in 25 ℃; The composition of seed culture medium is: 980 milliliters in NaCl10 in per 1000 milliliters gram, yeast powder 5 grams, peptone 10 grams and water.
3) ferment-seeded is pressed 6% of fermention medium volume and inserted in the fermentor tank, jar temperature 25 ℃, tank pressure 0.3kg/cm
2, stir speed (S.S.) 200rpm, air flow are 1: 0.5, fermented incubation time 36 hours.The fermention medium composition is: soluble-containing starch 0.1 gram, sucrose 60 grams, glucose 0.1 gram, casein 0.1 gram, yeast powder or yeast extract paste 0.1 gram, peptone 0.1 gram, feather meal Keratin sulfate 40 grams, K in per 1000 milliliters
2PO
44 grams, NaH
2PO
42H
2O 2 grams, MgCl
26H
2980 milliliters in O 0.2 gram and water.
Embodiment 3
1) adopts the method that is coated with or rules to glue golden yellow bacillus and be inoculated on the slant medium, cultivated 24 hours, obtain the rejuvenation thalline in 30 ℃ of rejuvenation; The composition of slant medium is: contain 980 milliliters in NaCl12 gram, yeast powder 6 grams, peptone 12 grams, agar powder 15 grams and water in per 1000 milliliters.
2) the rejuvenation thalline is inoculated in the liquid seed culture medium,, cultivated 14 hours under the 200rpm condition, obtain ferment-seeded in 30 ℃; The composition of seed culture medium is: 980 milliliters in NaCl12 in per 1000 milliliters gram, yeast powder 6 grams, peptone 12 grams and water.
3) ferment-seeded is pressed 6% of fermention medium volume and inserted in the fermentor tank, jar temperature 30 ℃, tank pressure 0.5kg/cm
2, stir speed (S.S.) 200rpm, air flow are 1: 0.8, fermented incubation time 30 hours.The fermention medium composition is: soluble-containing starch 0.1 gram, sucrose 0.1 gram, glucose 40 grams, casein 0.1 gram, yeast powder or yeast extract paste 0.1 gram, peptone 0.1 gram, hair Keratin sulfate 40 grams, K in per 1000 milliliters
2PO
40.8 gram, NaH
2PO
42H
2O 6 grams, MgCl
26H
2980 milliliters in O 2 grams and water.
Embodiment 4
1) adopts the method that is coated with or rules to glue golden yellow bacillus and be inoculated on the slant medium, cultivated 18 hours, obtain the rejuvenation thalline in 37 ℃ of rejuvenation; The composition of slant medium is: contain 980 milliliters in NaCl8 gram, yeast powder 5 grams, peptone 6 grams, agar powder 15 grams and water in per 1000 milliliters.
2) the rejuvenation thalline is inoculated in the liquid seed culture medium,, cultivated 12 hours under the 200rpm condition, obtain ferment-seeded in 37 ℃; The composition of seed culture medium is: 980 milliliters in NaCl8 in per 1000 milliliters gram, yeast powder 5 grams, peptone 8 grams and water.
3) ferment-seeded is pressed 6% of fermention medium volume and inserted in the fermentor tank, jar temperature 37 ℃, tank pressure 0.4kg/cm
2, stir speed (S.S.) 200rpm, air flow are 1: 1.5, fermented incubation time 24 hours.The fermention medium composition is: soluble-containing starch 0.1 gram, sucrose 0.1 gram, glucose 40 grams, casein 0.1 gram, yeast powder or yeast extract paste 0.1 gram, peptone 0.1 gram, wool keratin 40 grams, K in per 1000 milliliters
2PO
40.8 gram, NaH
2PO
42H
26 grams, MgCl
26H
2980 milliliters in O 2 grams and water.
Embodiment 5
1) adopts the method that is coated with or rules to glue golden yellow bacillus and be inoculated on the slant medium, cultivated 24 hours, obtain the rejuvenation thalline in 30 ℃ of rejuvenation; The composition of slant medium is: contain 980 milliliters in NaCl10 gram, yeast powder 5 grams, peptone 10 grams, agar powder 15 grams and water in per 1000 milliliters.
2) the rejuvenation thalline is inoculated in the liquid seed culture medium,, cultivated 20 hours under the 200rpm condition, obtain ferment-seeded in 30 ℃; The composition of seed culture medium is: 980 milliliters in NaCl10 in per 1000 milliliters gram, yeast powder 5 grams, peptone 10 grams and water.
3) ferment-seeded is pressed 4% of fermention medium volume and inserted in the fermentor tank, jar temperature 30 ℃, tank pressure 0.5kg/cm
2, stir speed (S.S.) 300rpm, air flow are 1: 1.5, fermented incubation time 30 hours.The fermention medium composition is: soluble-containing starch 0.1 gram, sucrose 0.1 gram, glucose 0.1 gram, casein 0.1 gram, yeast powder or yeast extract paste 0.1 gram, peptone 20 grams, feather keratin 40 grams, K in per 1000 milliliters
2PO
40.8 gram, NaH
2PO
42H
2O 6 grams, MgCl
26H
2980 milliliters in O 2 grams and water.
Embodiment 6
1) adopts the method that is coated with or rules to glue golden yellow bacillus and be inoculated on the slant medium, cultivated 24 hours, obtain the rejuvenation thalline in 30 ℃ of rejuvenation; The composition of slant medium is: contain 980 milliliters in NaCl10 gram, yeast powder 5 grams, peptone 10 grams, agar powder 20 grams and water in per 1000 milliliters.
2) the rejuvenation thalline is inoculated in the liquid seed culture medium,, cultivated 16 hours under the 200rpm condition, obtain ferment-seeded in 30 ℃; The composition of seed culture medium is: 980 milliliters in NaCl10 in per 1000 milliliters gram, yeast powder 5 grams, peptone 10 grams and water.
3) ferment-seeded is pressed 6% of fermention medium volume and inserted in the fermentor tank, jar temperature 30 ℃, tank pressure 0.5kg/cm
2, stir speed (S.S.) 250rpm, air flow are 1: 1.0, fermented incubation time 30 hours.The fermention medium composition is: soluble-containing starch 0.1 gram, sucrose 0.1 gram, glucose 20 grams, casein 0.1 gram, yeast powder or yeast extract paste 0.1 gram, peptone 20 grams, Keratin sulfate 0.1 gram, K in per 1000 milliliters
2PO
44 grams, NaH
2PO
42H
2O 6 grams, MgCl
26H
2980 milliliters in O 2 grams and water.
At last, it is also to be noted that what more than enumerate only is part specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.
The present invention can summarize with other the specific form without prejudice to spirit of the present invention and principal character.Therefore, no matter from which point, above-mentioned embodiment of the present invention all can only be thought can not limit the present invention to explanation of the present invention, claims have been pointed out scope of the present invention, and scope of the present invention is not pointed out in above-mentioned explanation, therefore, in implication suitable and any change in the scope, all should think to be included in the scope of claims with claims of the present invention.
Claims (3)
1, a kind of method of producing keratinase by liquid deep fermentation is characterized in that comprising the steps:
1) adopts the method that is coated with or rules to glue golden yellow bacillus and be inoculated on the slant medium, cultivated 18~36 hours, obtain the rejuvenation thalline in 18~37 ℃ of rejuvenation;
2) the rejuvenation thalline is inoculated in the liquid seed culture medium,, cultivated under 100~300rpm condition 18~36 hours, obtain ferment-seeded in 18~37 ℃;
3) ferment-seeded is pressed 5~15% of fermention medium volume and inserted in the fermentor tank, jar temperature 18~37 ℃, tank pressure 0.2~0.8kg/cm
2, stir speed (S.S.) 100~300rpm, air flow are 1: 0.3~2.0, fermented incubation time 18~60 hours; Described sticking golden yellow bacillus is Chryseobacterium L99, is preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, and preserving number is CGMCC No.2295.
2. the method for a kind of producing keratinase by liquid deep fermentation according to claim 1 is characterized in that the composition of described per 1000 milliliters of slant mediums is: NaCl5~15 grams, yeast powder or yeast extract paste 5~10 grams, peptone 5~15 grams, agar powder 10~20 grams, surplus are water.
3. the method for a kind of producing keratinase by liquid deep fermentation according to claim 1 is characterized in that the composition of described per 1000 milliliters of seed culture mediums is: NaCl5~15 grams, yeast powder or yeast extract paste 5~10 grams, peptone 5~15 grams, surplus are water.
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CN105567665A (en) * | 2016-03-25 | 2016-05-11 | 深圳市先康达生物科技有限公司 | Production method of high-efficiency keratinase |
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Non-Patent Citations (6)
Title |
---|
Characterization of a new keratinolytic bacteriumthatcompletely degrades native feather keratin. Alessandro Riffel et al.Arch Microbiol,Vol.179 . 2003 |
Characterization of a new keratinolytic bacteriumthatcompletely degrades native feather keratin. Alessandro Riffel et al.Arch Microbiol,Vol.179. 2003 * |
Purification and characterization of akeratinolytic metalloprotease from Chryseobacterium sp. kr6. Alessandro Riffel et al.Journal of Biotechnology,Vol.128 No.3. 2007 |
Purification and characterization of akeratinolytic metalloprotease from Chryseobacterium sp. kr6.Alessandro Riffel et al.Journal of Biotechnology,Vol.128 No.3. 2007 * |
Purification and properties of a keratinolytic metalloproteasefrom Microbacterium sp.. R.C.S. Thys and A. Brandelli.Journal of Applied Microbiology,Vol.101 No.6. 2006 |
Purification and properties of a keratinolytic metalloproteasefrom Microbacterium sp.R.C.S. Thys and A.Brandelli.Journal of Applied Microbiology,Vol.101 No.6. 2006 * |
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