CN100494122C - Multielement compound microbe bacterial fertilizer - Google Patents
Multielement compound microbe bacterial fertilizer Download PDFInfo
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- CN100494122C CN100494122C CNB2006101345419A CN200610134541A CN100494122C CN 100494122 C CN100494122 C CN 100494122C CN B2006101345419 A CNB2006101345419 A CN B2006101345419A CN 200610134541 A CN200610134541 A CN 200610134541A CN 100494122 C CN100494122 C CN 100494122C
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Abstract
The invention discloses a making method of polyvalent composite microbe fertilizer, which comprises the following steps: culturing bacteria under each fitful culture medium to prepare; allocating ferment culture medium; setting constant volume of ferment tank when the pH value is 7.4; sterilizing; weighing each 10% gel-shaped bacillus and blown-ball Azotobacter to inoculate at 30 deg.c with the ventilation quantity at 1: 0.5; stirring to culture for 12h; weighing 10% thin yellow streptomycete to culture for 10h under the same condition after detecting; culturing 7% huge bacillus and bacillus subtilis for 10h; culturing 10% brewery saccharomycete for 10h; weighing 30% composite bacteria of red coccus, capsula Rhodopseudomonas and marsh capsula Rhodopseudomonas and prunosus red coccus to culture at 30 deg.c for 10h without stirring; being first choice fertilizer to manufacture green pollution-free crop.
Description
Technical field
The present invention relates to a kind of organic fertilizer that adds cultivation bacterial classification, mycelia or other similar microorganisms, particularly a kind of multielement compound microbe bacterial fertilizer of containing.Be the first-selected fertilizer that vegetables, fruit tree, cash crop and food crop etc. are improved output, quality and resistance against diseases.
Background technology
The evolution of fertilizer is the chemical fertilizer from initial farm manure to elements such as nitrogenous, phosphorus, potassium, calcium, magnesium, five, trace element fertilizer appears in the sixties, occurred plant-growth regulator the 1970s and 1980s again.However, still general a large amount of chemical fertilizer, agricultural chemicals of using improve output and disease resistance on the agricultural for a long time, promote agriculture production.Yet chemical pesticide has been a well known fact to the pollution of environment, to the destruction of ecotope.A large amount of uses of chemical pesticide are except that direct or indirect poisoning that can directly cause people and animals even death, also because its residual on soil and crop, and soil, underground water, river, lake etc. are polluted, bring harm especially for descendants's living environment and health.Desertify owing to the life-time service chemical fertilizer not only makes soil compaction in addition, but also can cause the barren soil fertility of soil property to descend, based on above-mentioned situation, the Chinese government prohibites use or restriction use to some toxicity and the big chemical pesticide of residual quantity.Also Just because of this, also come out one after another such as multiple-effect plant source pesticide, bacterial manure more in recent years, owing to itself be organism, a large amount of trace elements are arranged in the substratum, most of bacterial manure has fixed nitrogen or phosphorus decomposing, potassium decomposing effect.But present bacterial manure mainly contains two aspect shortcomings: one is that bacterial classification is single as vinelandii, phosphorus bacteria or potassium bacterium, and these single bacterium are poor to the deleterious ion of crop such as nitrite anions, sulfate radical receptivity, cause soil acidification; Phosphorus bacteria, potassium bacterium are because after being manured into soil on the other hand, phosphorus in the soil, potassium component are seldom, cause phosphorus bacteria, potassium bacterium can't give full play to the effect of phosphorus decomposing, potassium decomposing, and then cause the DeGrain of the little and quality improving of the amount of increase in production of crop, limited to and it is applied.
Summary of the invention
The present invention is directed to the problem that prior art exists, a kind of multielement compound microbe bacterial fertilizer is provided, vegetables, fruit tree, cash crop and food crop etc. are had tangible volume increase and improve the quality effect, good to environment compatibility; And can promote crop growth, ripen in advance, fertilizer efficiency period is long; The acid-basicity of activating soil, adjusting soil, the remaining agriculture chemical in the soil of can also degrading, inhibition disease and environment purification; Improve utilization rate of fertilizer, phosphorus decomposing, potassium decomposing and fixed nitrogen, environmental protection simultaneously is nuisanceless.
The selected bacterial classification of the present invention all derives from Chinese agriculture microbial strains preservation administrative center (hereinafter to be referred as ACCC), and provides and purchase bacterial classification and prove.
Bacillus megaterium (Bacillus megaterium de Bary) ACCC10011, subtilis Bacillus subtilis (Ehrenberg) Cohn ACCC10619, bacillusmusilaginosiengineering (Bacillus mucilaginosus Krasilnikov) ACCC10007, blown-ball Azotobacter (Azotobacterchrooccum Bei jerinck) ACCC10015, rhodococcus (Rhodococcus codochrous) ACCC11827, pod membrane Rhodopseudomonas (Rps.capsulatus), swamp Rhodopseudomonas (Rhodosedoseudomouas padustris) ACCC10649, prunosus red coccus (Rhodococcusrhodochrous) ACCC11828, streptomyces microflavus Streptomyces microflavus (krainsky) Wsksman et Henrici) ACCC40027, S. cervisiae (Saccharomyces cervisiae Meyenet Hansen) ACCC20063
Cultivate each selected bacterial classification
1. the cultivation of bacillus megaterium (ACCC1011)
Culture medium preparation: get 50g Semen Maydis powder water furnishing pasty state by proportioning, after the heated and boiled with Nacl2g, peptone 1g, sucrose 10g phased soln, transfer PH=7.2, the back inoculation was cooled off in the 121 ℃ of sterilizations of triangular flask of packing in 30 minutes, last shaking table culture temperature 30 ℃/200 is changeed cultivation 18 hours, after inspection, strain liquid is standby as producing;
2. the cultivation of subtilis (ACCC10619)
Culture medium preparation: get sucrose 8g, Nacl 3g, peptone 3g, extractum carnis 1g adjusting soluble in water PH=7.2, go into 121 ℃ of sterilizations of triangular flask cooling back inoculation in 30 minutes, last shaking table 200 changes/30 ℃, 16-18 hour, before the inspection thalline is born in the gemma growth, there is no contaminated bacteria, it is standby to do seeding liquid;
3. the cultivation of bacillusmusilaginosiengineering (ACCC10007)
Culture medium preparation: get sucrose 10g, Nacl 1g, dipotassium hydrogen phosphate 2g, sal epsom 0.5g, ammonium sulfate 1g, CaCO
33g is soluble in water, and PH=7.2 goes into 121 ℃ of triangular flasks, sterilization in 30 minutes, cooling back inoculation, last shaking table 28 ℃/200 changes to be cultivated 48 hours, through inspection do not have the bacterium of mixing, thalli growth is good, qualified standby;
4. the cultivation of blown-ball Azotobacter (ACCC10015)
Substratum preparation: get dipotassium hydrogen phosphate 1g, CaCO
32g, iron trichloride 0.1g, sal epsom 0.2g, Nacl 0.2g, Sodium orthomolybdate 0.005g, sucrose 10g are water-soluble, transfer PH=7.0, and sterilization, shaking table 30 ℃/200 is gone up in the inoculation of cooling back changeed 48 hours, and is qualified standby through examining;
Rhodococcus (ACCC11827), capsula Rhodopseudomonas, Rhodopseudomonas palustris (ACCC10649),
And prunosus red coccus (ACCC11828) mixed culture
Substratum preparation: get ammonium sulfate 1g, Nacl 2g, dipotassium hydrogen phosphate 0.5g, sal epsom 0.2g, iron trichloride 0.05g, copper sulfate 0.05mg, sal epsom 0.05mg, zinc sulfate 1mg, peptone 2g, yeast extract paste 0.1g. are soluble in water, PH=7.5 goes into 115 ℃ of bottle sterilizations, be cooled to 35 ℃ of inoculations in 15 minutes, illumination 30-35 ℃, 72-120 hour, get wine-colored mixed bacteria liquid, qualified standby through examining;
6. the cultivation of streptomyces microflavus (ACCC40027)
The substratum preparation; Get Semen Maydis powder 60g and be heated into pasty state with the less water dissolving, again it is dissolved in the culture medium solution of sal epsom 0.2g, dipotassium hydrogen phosphate 0.25g, Sodium phosphate dibasic 1g, transfer PH=7.4 to go into 121 ℃ of triangular flasks, sterilization in 30 minutes, 28 ℃/90-200 of shaking table is gone up in cooling back inoculation to be changeed, and 72 hours standby through examining after qualified;
Above culture medium preparation all is the consumption with the liquid medium of preparation 1000ml.
7. the cultivation of S. cervisiae (ACCC20063)
Substratum preparation: the soybean sprout that 100g cleans is gone into to boil in the 1000ml water 30 minutes, get in the liquid medium that 100ml bean sprouts juice is dissolved in yeast extract paste 4g, sucrose 15g, Nacl2g, peptone 1g, PH=6.5-6.7, sterilize 121 ℃, cooling back inoculation in 30 minutes is gone up shaking table and is spread cultivation and 28-30 ℃/200 change, and it is standby to detect qualified back in 16-20 hour; Finishing pure strain cultivates in order to producing.
Nutrition source to above various substratum there is no special regulation, can make to contain carbon source and other nutrition sources that is usually used in microorganism culturing in the substratum.Wherein carbon source can be starch, dextrin, glycerine, glucose, sucrose etc., and nitrogenous source can be peptone, soyflour, meat extract, rice sugar, wheat skin, corn steep liquor, ammonium salt, nitrate and other organic or inorganic nitrogenous compounds.Then can suitably add some inorganic salts as for other nutrition source.The metal-salt of salt, phosphoric acid salt and potassium, calcium, zinc, sodium, iron and so on for example.
Culture condition such as temperature, time be there is no strict restriction, being suitable for the using growth of bacterium to be as the criterion, and to select using the highest condition of bacterium output for well.PH scope as substratum can be 5-9, especially with near neutral for well, culture temperature should be at 25-35 ℃, certain described condition also should be according to the kind of use bacterial strain, reaches external conditions etc. and carries out suitable adjusting, to obtain best effect.
By the culture of above-mentioned gained,, just can make the purpose product of composite multi-component bio-bacterial manure by some appropriate means.And contain biological bacteria more than 200,000,000/ml or 200,000,000/g.
Embodiment
Below further be illustrated with regard to manufacturing of the present invention, experimental example.
Make example
With Semen Maydis powder 10 ‰, sucrose 15 ‰, dipotassium hydrogen phosphate 0.02 ‰, CaCo
30.3% ‰, the ratio of sal epsom 0.02 ‰ is made fermention medium, and PH=7.4 before the sterilization moves in the fermentor tank and carries out constant volume, and carry out reality and disappear, 121 ℃, sterilized in 30 minutes, to be cooledly inoculate the above-mentioned polynary bacterial classification for preparing during to 30 ℃ successively.Getting constant volume each bacillusmusilaginosiengineering (ACCC10007) and blown-ball Azotobacter (ACCC10015) of 10% inoculates, 30 ℃ of culture condition temperature, molten foster 1:0.5 (V/V) air flow, 220 rev/mins of stirrings, cultivate 12 hours qualified after testing after, inoculating streptomyces microflavus (ACCC40027) inoculum size is 10% of constant volume, and with the same terms cultivation 10 hours, after qualified after testing, regulating PH=7.0 inoculates with the same terms cultivation 18 hours with each 7% bacillus megaterium (ACCC10011) of constant volume and subtilis (ACCC10619) again, after qualified after testing, the S. cervisiae of getting constant volume 10% amount again inoculates, with above-mentioned the same terms cultivate 10 hours through examine qualified after, get the rhodococcus (ACCC11827) of constant volume 30% inoculum size again, capsula Rhodopseudomonas, the mixed culture bacterial classification of swamp Rhodopseudomonas (ACCC10649) and prunosus red coccus (ACCC11828) is inoculated, its culture condition is 30 ℃ of temperature, do not support and do not cultivate 10 hours under the agitation condition to molten, detect every kind of viable count 〉=200,000,000/ml, PH=5.0-7.0 is finished product, and meets the Ny227-1994 standard code.
If product adding solid phase of the present invention such as careless carbon, zeolite and so on carrier guarantees that its every gram product contains 200,000,000 above viable counts and both had been the solid phase product.
Experimental example
Multicomponent biological complex bacterial manure of the present invention by the academy of agricultural sciences, Jining City in May, 03 begin on November 30th, 03 to 17 kind of plant such as corn, soybean, cotton, paddy rice, tomato, capsicum, peanut, kidney bean, cauliflower, Chinese cabbage, grape, Radix Isatidis, the living poplar of speed, Triploid of Populus Tomentosa, trifolium, comprise that land for growing field crops farm crop, vegetables, fruit, Chinese medicinal materials, forest, lawn etc. test respectively, its result is as follows:
One, test is handled
1. select the plot of soil fertility, management, growing way unanimity, its area is decided because of crop species, and field crop is more than 10 mu, and vegetables, fruit are not established repetition more than 1 mu.
2. to the processing of sample:
Handle I biological composite bacterial manure foliage-spray, concentration is 300 times, respectively at seedling phase, bud stage, the sprinkling of fruit phase, totally four times, wherein sprays twice seedling stage, 10 days at interval.
Handling II processing I+ seed soaking (seedling) concentration is 200 times, 1 day time or 12 hours.
Handle III contrast (CK), spray equivalent clear water.
Two, investigation method
1. biology investigation is taked diagonal lines 5 point samplings, every some 1M
2(or be equivalent to 1M
2The strain number), record seedling-growing time, growing way, plant height, leaf look, budding time, the disease situation etc. of blooming compare with contrast.
2. output investigation, the sampling method area is investigated with biology, surveys and receives every output, calculates the increase and decrease productive rate, statistics output ratio.
Experimental example 1. kinds: little assorted 56, the summer, Chinese cabbage was in sowing on May 9, by handling I respectively at June 11, June 21 and July 1 totally three times, 5 average output value X=4.38kg foldings of harvest time treatment zone on July 10 per mu yield 2917kg; CK district 5 mean value X=3.69kg foldings per mu yield 2458kg, then
Spray 3 times 6 barrels of bacterium liquid, 9 yuan of 1.5 yuan of (down together) meters of every barrel of expenses of labour; 6 bags of 3 microbial inoculums, 9 yuan of 1.5 yuan of/bag (down with) meters; Add up to 18 yuan/mu, volume increase 459kg/ mu by every kg0.3 unit, adds up to 137.7 yuan, and input-output ratio is 1:7.65.Biology is observed basic indifference.
Example 2. kidney bean Xiayang 50, handle: respectively at June 11, June 21, spraying on July 1 three times, CK sprays the equivalent clear water simultaneously, and July 13, results finished, statistics output, 5 samplings are got 1 meter of duplicate rows at every.5 mean value 2692g of treatment zone, folding per mu yield 1792.9kg; CK district 5 mean value 2524g foldings per mu yield 1681.0kg.
9 yuan of three medications, add up to 18 yuan by artificial 9 yuan; Volume increase 111.9kg, by every kg1.0 unit, 111.9 yuan of input-output ratios of Renminbi are 1:6.22.Biology is observed indifference.
Example 3. capsicums are middle green pepper No. 8, Zhong Wang village, Li Ge town, Yutai, place.The field planting time is June 6, mu 400 piers (0.6 * 0.27-0.28); Preceding crop is a garlic; Handle: spraying 300X, first inferior to June 25, every 10 days once totally four times later on; 0.2/ mu in CK district spray equivalent clear water, 5 samplings of treatment zone, every some 1M
2(6 pier).5 mean yields of treatment zone are 5981.4g folding per mu yield 3990.0kg; 5 mean yields in CK district are 5191.08g, folding per mu yield 3462.4kg.
Calculate input-output ratio: 12 yuan of 4 fertilising labour costs, microbial inoculum takes 12 yuan, adds up to 24 yuan/mu; Volume increase 527.6kg counts 316.56 yuan by every kg0.6 unit, and input-output ratio is 1:13.19.
Biology is observed and shown: treatment zone eqpidemic disease, anthrax obviously are lighter than the CK district.Fruit is big, and is bright-colored, and the receipts phase prolongs 10 days.
The test of example 4. tomatoes, in the village, Shen, Yutai Li Ge town, preceding stubble garlic, density 0.4 * 0.6m.2700 strain/mus.Handle 300 times of I microbial inoculum sprayings, 10 days at interval, totally four times, contrast CK equivalent clear water.5 samplings of diagonal lines are got duplicate rows at every, and totally 6 strains are in the some product.5 average out to 5086.8g of treatment zone, folding per mu yield 2289.1kg; 5 the average out to 4307.6g in CK check plot, folding per mu yield 1938.1kg
Mu volume increase 351kg, mu drops into 24 yuan/mu, presses 0.6 yuan of per kilogram, closes and increases income 210.6 yuan, and input-output ratio is 1:8.78; Biology is observed and is found early blight, and the late blight treatment zone obviously is lighter than the CK district.
Example 5. is at five Li Tunlin, kyoto grape, and the equidistant sampling of parallel lines, totally 5 points, I is handled in every 2 strain, 300 times of sprayings four times, 5 mean yields are that 21.12kg is by every mu 400 strain folding per mu yield 4224kg; 5 mean yields in CK check plot are 18.65kg, folding per mu yield 3730kg
Input-output ratio: four sprayings, mu are with artificial 40 yuan, and microbial inoculum 4 * 6=24 bag adds up to 76 yuan for 36 yuan, and volume increase 494kg adds up to 592.8 yuan by 1.2 yuan/kg, and input-output ratio is 1:7.8.
The biological observation shows that rainwater in 2003 is many, and disease is generally serious, and the disease of treatment zone obviously is lighter than contrast CK district, alleviates 28.0% as anthrachose of grape, and white rot alleviates 23.6%, and the treatment zone fruit is neat, and the leaf look dark green.
Example 6. corn Shandongs list 981, in test farm, academy of agricultural sciences, city, 5 samplings of diagonal lines, every 40 strain are handled the I spraying in the some product, and 5 are all produced is 6.21kg, presses mu 4000 a strains folding per mu yield 627kg; Handle II=and handle the I+ seed soaking, 5 are all produced is that 6.41kg rolls over per mu yield 641kg; Control group CK district, 5 are all produced is 5.73kg, folding per mu yield 573kg.
Stimulation ratio is respectively handles I7.73%; Handling II is 10.61%, input-output ratio: handling I4 spraying is 24 yuan, handle II seed soaking cost and be 3 yuan/mu and add 24 yuan, adds up to 27 yuan, increase production be respectively 48kg, 68kg by 1.2 yuan/kg respectively synergy be that 57.6 yuan, 81.6 yuan, its input-output ratio are respectively 1:2.4 and 1:3.02;
The biological observation: treatment zone was emerged 1 day in advance, and root system is flourishing than the CK district, and the leaf look dark green, and rust is light.
Test farm, academy of agricultural sciences, example 7. city, 5 samplings of Shandong beans 12 diagonal lines, every some 1M
2, handle 5 of I and all produce to 225.6g, roll over per mu yield 170.23kg volume increase 7.7%; Handle 5 of II and all produce 248.2g; Folding per mu yield 165.30kg, volume increase 4.9%, 5 in CK district all produces and is 236g, folding per mu yield 157.18kg.
Input-output ratio: handle 24 yuan of I costs, volume increase 13.05kg/ mu, by 2.8 yuan/kg, 36.54 yuan; Handling II volume increase 8.12kg/ mu, is 22.74 yuan by 2.8 yuan/kg, and input-output ratio is respectively 1:1.52 and 1:0.85.The biological observation do not have obviously difference.
Example 8. is in Yutai Li Ge town, 1.5 mu of Taiwan holy girl's areas, CK0.2 mu.In seeding in nursery bed on May 16, field field planting on June 10,2000 strain/mus sprayed for the first time 300 times of bacterium liquid on June 20, and at interval 10 days once totally four times later on, July 18 began to gather.
5 methods are adopted in sampling, every 4 strain, and to by the end of August, every some mean yield of accumulative total is 6760.2g folding per mu yield 33804.1kg; 5 mean yields in CK district are 6122g, folding per mu yield 3060kg, volume increase 9.44%.
Input-output ratio: 36 yuan of mu costs, 320.1kg is by 2 yuan/kg in the mu volume increase, increases income 638.8 yuan, and input-output ratio is 1:17.72.The biological observation: the treatment zone growth is vigorous, and disease is light, and the receipts phase prolongs 5 days.
Example 9. is at temple in third of the twelve Earthly Branches town, Wenshang County Cui Xinzhuan, and peanut varieties is a white sand.Sow on June 18th, 03, neat June 28 seedling time, wherein handle II promptly soak seed 12 hours+spray four times in tidy seedlings output on June 27.Surveyed September 23 and produce, by five-spot, every some 1M
2By the some product.5 of processing I all produce and are 505.6g, folding per mu yield 337.2kg; Handle II, 5 are all produced is 528.89, folding per mu yield 352.7kg; Contrast CK district, 5 are all produced is 464.6g, folding per mu yield 309.9kg.Stimulation ratio is divided into handles I8.1%, handles II 12.1%; Input-output ratio: handle 24 yuan/mu of I costs; Handle 27 yuan/mu of II costs, volume increase is respectively 27.3kg/ mu, 42.8kg/ mu, by 4.0 yuan/kg, increases income 149.2 yuan and 171.2 yuan/mu respectively, and input-output ratio is respectively 1:6.22,1:6.34; The biological observation: the treatment zone peanut leaf is dark green, and leaf spot reduces by 30.6%, and thousand seed weight increases 36.1g.
Example 10. cauliflower kinds refreshing good 60 in the court vegetables institute were experimental field sowed June 26, field planting on July 24, seeding row spacing 0.4 * 0.55 (M), mu 3000 strains.Handle I, handle II, CK district spray clear water.
5 samplings are fixed 6 strains at every, in the some product.
5 of processing I all produce and are 3499.8g, folding per mu yield 1749.9kg; Handle II, 5 are all produced 3657.8g, folding per mu yield 1828.9kg; 5 in CK district all produces 3106.4g, folding per mu yield 1603.2kg.
Stimulation ratio is respectively handles I8.02%, handles II 12.34%.
Input-output ratio: cost is handled I24 unit/mu, volume increase 146.7kg/ mu; Handle 27 yuan/mu of II costs, volume increase 225.7kg/ mu is scored by 1.5 yuan/kg and is not increased income 220.05 yuan/mu, 338.55 yuan/mu input-output ratios and be respectively 1:9.17 and 1:12.54; The biological observation shows that treatment zone emerges neat strongly, and what wherein seed soaking was handled emerged in advance in 1 day, and root system is flourishing than the CK district, and bouquet is neat, the bead compactness.
Shen Zhuan village, example 11. Yu Lige town, cotton variety grinds 16 for the Shandong cotton, 10 mu of test area, contrast CK1 mu.Sprayed bacterium liquid for the first time on June 25, sprayed once totally four times every 10 days afterwards, by the young ginnings of some statistics.Handle 5 in I district and all produce 265.2g, folding per mu yield 176.9kg; CK district 237.2g, folding per mu yield 158.2kg, stimulation ratio is 10.6%; Input-output ratio: 24 yuan/mu of costs, volume increase 18.7kg/ mu increases income 123.42 yuan/mu by 6.8 yuan/kg, and input-output ratio is 1:5.14.Biological observe except that the treatment zone cotton premature senescence gently, other no significant differences.
Example 12. is tested Leaf of Indigowoad, Radix Isatidis, 30 mu of test sites, 3 mu of CK check plots at Zhang village, Liangshan County Xiao Anshan town.From July 1, spray for the first time every interval 10 days 1 time, totally four times.Leaf, root list are received single meter, 5 samplings of diagonal lines, every some 1M
2, 5 are all produced Leaf of Indigowoad (doing) 121.4g folding per mu yield 80.85kg, Radix Isatidis (aquatic foods) 842.8g.Folding per mu yield 561.3kg; 5 in CK district all produces Leaf of Indigowoad (doing) 106.0g, folding per mu yield 70.60kg; Radix Isatidis (aquatic foods) 790.8g folding per mu yield 525.67kg.The Leaf of Indigowoad stimulation ratio is 12.7%, Radix Isatidis 6.2%; Input-output ratio is 1:3.53.Because this year, rainwater was many, Radix Isatidis ground ponding reaches 3 days, and minimizing throughout the year is nearly 50%, so the test in this year can only show a kind of volume increase trend.
Example 13. rice tests are being explained village Gao Cun, and kind is obstructed No. 6 in advance, and treated I handles II respectively, 24 hours+processing I of 200 times of seed soaking and contrast CK district.
Press diagonal lines 5 point samplings, every some 1M
2, carry out actual measurement, calculate rice output by point.
Handle 5 of I and all produce 805.2g, folding per mu yield 537.1kg; Handle 5 of II and all produce 821g, folding per mu yield 547.6kg; 5 of CK of contrast all produce 700.69, folding per mu yield 467.3kg.
It is 13.0% that stimulation ratio is handled I, and handling II is 14.7%
Input-output ratio: handle I mu and increase 24 yuan of costs, volume increase 69.8kg/ mu; Handle II, mu increases 28 yuan of volume increase of cost 80.3kg/ mu.Do not increase income 104.7 yuan and 120.45 yuan by 1.5 yuan/kg score.Input-output ratio is respectively 1:3.65 and 1:3.74.
Illustrate: this year, rainfall amount increased more than 80% more throughout the year, and rice disease is extra heavy, and particularly banded sclerotial blight, rice blast are very popular, and fell ill evening than the CK district in processing I, II district, and disease is light, and dead ears rate, lodging obviously reduce, and however thousand seed weight also increases 6.2g.
Example 14. Chinese cabbage Shandongs white 16 in academy of agricultural sciences vegetables institutes experimental field, the sowing time is live on August 13rd, 03, density 2200 strain/mus, harvest time on November 20th, 03.Handling I, handling II is seed soaking+processing I, CK spray equivalent clear water.
Five point samplings are got 5 strains at every, and statistics hair dish output is unit with the point when results, handle the I5 point and all produce 15.74kg, folding per mu yield 6925.6kg; Handle 5 of II and all produce 16.8kg, folding per mu yield 7392kg; CK district 13.84kg, folding per mu yield 6089.6kg; Produce gaining rate and be respectively processing I13.0%, handle II17.62%.Input-output ratio: handle spraying 24 yuan of microbial inoculums for I4 time, volume increase 836kg/ mu by 0.3 yuan/kg, increases income 250.8 yuan/mu; Handle 27 yuan/mu of II costs, volume increase 1302.4kg/ mu increases income 390.7 yuan/mu, and input-output ratio is respectively 1:10.45 and 1:14.47.
The biological observation shows that treatment zone leaf look dark green, and it is vigorous to grow, and oidium, soft rot sickness rate reduce 31.2% and 27.8% respectively.
The livings poplars of example 15. speed are tested kinds 107, transplant a cutting life in 1 year, on farm, lake, Nanyang.Handle I, 5 investigation of contrast CK diagonal lines, every 5 strain is handled the thick one meter eminence diameter cm of I stem, 5 average diameter 17.448cm.5 average 287.07cm of the height of tree, contrast CK5 point average diameter 15.492cm, 5 average 266.40cm of the height of tree, 5 on average increase rough and careless is 11.2%, the rate of increasing is 7.21%.The biological observation shows that the treatment zone fallen leaves than 3-5 days evening of control group, have fallen in 90%.
The test of example 16. Triploid of Populus Tomentosa, stump annual (root life in 2 years) farm, lake, Nanyang.Handle I, contrast CK district five point samplings, 51 meters eminences, the thick average 28.37cm of stem.Height of tree average 497.05cm, thick 5 the average 25.02cm of contrast CK district's stem, height of tree average 452.41cm.Increase rough and carelessly 10.82%, increase rate 5.57%; The no significant difference of biological observation.
Example 17. trifolium test kind is not quite clear, in Wenshang County Nan Wang town, handles I, 5 samplings of contrast CK, every some meter fresh weight, CK spray equivalent clear water.5 counterpoise 541.98g of treatment zone, 5 counterpoise 517.94g of contrast CK; Stimulation ratio is 4.44%.The biological difference of observing is not obvious.
Brief summary
1. from the situation of the application products of the present invention of 17 kinds of crops being carried out, different effect of increasing production is arranged all.Stimulation ratio is having 14 kinds of crops such as summer Chinese cabbage, capsicum, tomato, grape, corn, cherry tomato, peanut, cauliflower, cotton, Leaf of Indigowoad, paddy rice, Chinese cabbage, the living poplar of speed, Triploid of Populus Tomentosa more than 10%.
2. see with input-output ratio, surpass or 10 kinds of crops such as summer Chinese cabbage, kidney bean, capsicum, tomato, grape, cherry tomato, peanut, cauliflower, cotton, Chinese cabbage are arranged, wherein surpass 4 kinds of crops such as capsicum, cherry tomato, cauliflower, Chinese cabbage of 1:10 near the kind of 1:5; The living poplar of speed, Triploid of Populus Tomentosa, trifolium etc. can't be calculated input-output ratio temporarily.
3. from crop species, effect of the present invention, cash crop are better than food crop, and are best with the vegetables effect, have the economic benefit of highly significant, and taking second place also has favorable economic benefit for cash crop, fruit tree etc.
4. show over-ground part (Leaf of Indigowoad) stimulation ratio 12.7% with Radix Isatidis, Leaf of Indigowoad test-results, underground part (Radix Isatidis) stimulation ratio only is 6.2%, also may with today rainfall amount big, experimental field be subjected to water logging, the gas permeability of soil is poor, and the underproduction is seriously relevant, remains further to be tested to investigate.
5. analyze from using method, seed soaking adds the processing that the processing effect of increasing production that sprays is better than only spraying 4 times, is respectively 17.6% and 13% as Chinese cabbage; Paddy rice is 14.7% and 13%; Cauliflower is 12.34% and 8.02%; Peanut is 12.1% and 8.1%; Corn is 10.61% and 7.73%; Having only the soybean exception is 4.9% and 7.7%, and may be that soybean seeds is not water-fast soaks, and occurs due to the cracking.
6. from the test result analysis of two kinds of willows, product of the present invention is better than increasing effect for the thick growth effect of willow stem, increases rough and careless 11.21% and increase rate 7.21% as the living poplar 107 of speed; Triploid of Populus Tomentosa is respectively 10.82% and 5.57%, its rising tendency basically identical.
In sum, product of the present invention not only has tangible production-increasing function and high economic benefit to vegetables, fruit tree, cash crop and food crop etc., but also has the waterlogging effect of certain disease resistance, lodging and drought resisting, and is short precocious, prolong harvesting time and improve the quality effect.
Product of the present invention compared with prior art owing to adopted bacterium, actinomyces, saccharomycete and photosynthetic The polynary bacterium such as bacterium is cultivated stage by stage and is composited, and through evidence have pair vegetables, fruit tree, industrial crops and Cereal crops etc. have obvious production-increasing function and higher economic benefit. And has certain disease resistance, anti-Waterlogging, the lodging-prevention ability of drought, short precocious, improve a quality improvement 1-2 grade; And effectively solve the soil plate Knot improves the soil cementation structure and increases beneficial microbe and the content of organic matter in the soil, improves soil physical and chemical property; Effectively decompose multiple curing nutrient in the soil, improve utilization rate of fertilizer and conversion ratio, prevent soil acidification, alkali Change, long-term use can strengthen the soil property fertility, and deteriorating pesticide residue, chemical fertilizer are to produce the green non-pollution agricultural production The available fertilizer of product.
Claims (1)
1. multielement compound microbe bacterial fertilizer is characterized in that being prepared by following method:
A). it is standby to cultivate selected bacterial classification in substratum separately respectively;
B). preparation fermention medium Semen Maydis powder 10 ‰, sucrose 15 ‰, dipotassium hydrogen phosphate 0.02 ‰, CaCO
30.3 ‰, sal epsom 0.02 ‰, transfer PH=7.4 to go into the fermentor tank constant volume, sterilization, getting standby polynary bacterial classification in the time of 30 ℃ successively inoculates, getting constant volume each bacillusmusilaginosiengineering (Bacillusmucilaginosus Krasilnikov ACCC10007) and blown-ball Azotobacter (Azotobacter chrooccumBeijerinck ACCC10015) of 10% inoculates, 30 ℃, the air flow of molten foster 1:0.5, stir down and cultivated 12 hours, get streptomyces microflavus (Streptomyces microflavus (krainsky) the Wsksman et Henrici ACCC40027) inoculation of constant volume 10% after experience is qualified, it was cultivated 10 hours with identical culture condition, use the bacillus megaterium (Bacillus megaterium deBary ACCC10011) and subtilis (Bacillus subtilis (Ehrenberg) Cohn) ACCC10619 of constant volume 7% after experience is qualified more respectively) inoculation, cultivated 10 hours with identical culture condition, after experience is qualified, get S. cervisiae (the Saccharomyces cervisiae Meyen et Hansen ACCC20063) inoculation of constant volume 10% again and cultivated 10 hours with the same terms, after experience is qualified, get the rhodococcus (Rhodococcus codochrous ACCC11827) of 30% constant volume inoculum size, capsula Rhodopseudomonas (Rps.capsulatus), the mixed strains of swamp Rhodopseudomonas (Rhodosedoseudomouas padustris ACCC10649) and prunosus red coccus (Rhodococcus rhodochrous ACCC11828) is inoculated, do not give molten supporting at 300C, agitation condition was not cultivated 10 hours down, became every milliliter of every kind of viable count of inspection to be not less than 200,000,000 for qualified.
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