CN100490826C - 多联活性双歧杆菌产品的制备方法 - Google Patents

多联活性双歧杆菌产品的制备方法 Download PDF

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CN100490826C
CN100490826C CNB2005100355847A CN200510035584A CN100490826C CN 100490826 C CN100490826 C CN 100490826C CN B2005100355847 A CNB2005100355847 A CN B2005100355847A CN 200510035584 A CN200510035584 A CN 200510035584A CN 100490826 C CN100490826 C CN 100490826C
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bifidobacterium
dry powder
cfu
bifidobacteria
product
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CN1891236A (zh
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夏枫耿
李运南
谭颖嫦
彭中健
杜少平
王丽霞
张学智
彭维
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Guangzhou Gaoxinguang Microbial Technology Co ltd
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GUANGZHOU CITY INSTITUTE OF MICROBIOLOGY
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Abstract

本发明是一种多联活性双歧杆菌产品的制备方法。双歧杆菌是与人类健康关系极为密切的一种正常生理性细菌。该产品的制备是根据不同人群的需要，用不同种类的双歧杆菌活菌粉制备成多联双歧杆菌活菌干粉，在这些多联双歧杆菌活菌干粉中加入促进增殖的低聚糖类，以及对人体有重要意义的氨基酸等物质所组成。本发明之产品具有常温贮存期长、双歧杆菌活菌数高和耐胃酸性强等优点。

Description

多联活性双歧杆菌产品的制备方法
技术领域
本发明涉及一种生理性细菌的制备方法，特别是一种多联活性双歧杆菌的制备方法。
背景技术
双歧杆菌是与人类健康关系极为密切的一种正常生理性细菌，但是随着环境的污染及抗生素的滥用，使得正常人体该菌群含量大大降低，严重影响人们的身体健康。为了造福人民，研制能常温贮存期长、双歧杆菌活菌数高和耐胃酸性强的双歧杆菌产品就显得尤为需要。
经过多年的研究和探索，我们利用全新的发酵工艺培养出不同种类的双歧杆菌，并经冻干的活菌粉，包括婴儿双歧杆菌(Bifidobacterium infantis)、短双歧杆菌(Bifidobacterium breve)、两歧双歧杆菌(Bifidobacteriumbifidum)、青春双歧杆菌(Bifidobacterium adolescentis)、长双歧杆菌(Bifidobacterium longum)、乳杆菌(L bulgaricus)、嗜热链球菌(Sthermophilus)等。
发明内容
根据不同人的需要用不同种类的双歧杆菌活菌粉制备成多联双歧杆菌活菌干粉，同时在这些多联双歧杆菌活菌干粉中加入促进该类菌群增殖的低聚糖类，以及对人体有重要意义的氨基酸等物质组成为本发明之多联活性双歧杆菌产品。
本发明之配方由：
多联双歧杆菌活菌干粉       X％
脱脂奶粉                   30％
低聚果糖                   10％
乳糖                       40-X％
麦芽糊精                   15％
丙氨酸                     1.5％
缬氨酸                     1.5％
苏氨酸                     1.5％
维生素B2                   0.5％组成，产品指标：活菌数≥1.0×106cfu/g，
制备时必须在洁净度为100级的环境条件中操作。
本发明产品具有常温贮存期长、双歧杆菌活菌数高和耐胃酸性强等优点。
具体实施方式
下面结合实施例详述。
实例：
制备1000g多联活性双歧杆菌产品，活菌数为1.0×108cfu/g，且由短双歧杆菌、婴儿双歧杆菌、长双歧杆菌和乳杆菌四联菌组成，含菌比例基本为1:1:1:1。
设定短双歧杆菌干粉活菌数为2.3×109cfu/g，婴儿双歧杆菌干粉活菌数为1.3×109cfu/g，长双歧杆菌干粉活菌数为2.03×1012cfu/g，乳杆菌干粉活菌数为1.63×108cfu/g。
根据产品配方，在洁净度为100级的环境条件中并经灭菌后分别称取：
短双歧杆菌干粉：1.0×108÷4÷(2.3×109)×1000＝10.9g
婴儿双歧杆菌干粉：1.0×108÷4÷(1.3×109)×1000＝19.2g
长双歧杆菌干粉：1.0×108÷4÷(2.03×1012)×1000＝0.02g
乳杆菌干粉：1.0×108÷4÷(1.63×108)×1000＝24.3g
即多联双歧杆菌干粉为：10.9g+19.2g+0.02g+24.3g＝54.42g；因此得：
多联双歧杆菌活菌干粉X＝54.42÷1000×100＝5.44，那么，
乳糖：                       (40-5.44)％×1000＝345.6g；
脱脂奶粉：                   30％×1000＝300g；
低聚果糖：                   10％×1000＝100g；
麦芽糊精：                   15％×1000＝150g；
丙氨酸、缬氨酸、苏氨酸各为： 1.5％×1000＝15g；
维生素B2：                   0.5％×1000＝5g。
将上述配方之物质混合均匀，无菌包装即得1000g多联活性双歧杆菌产品。

Claims (2)

1、一种多联活性双歧杆菌产品的制备方法，其特征在于：该产品配方由
多联双歧杆菌活菌干粉                X％
脱脂奶粉                            30％
低聚果糖                            10％
乳糖                                40-X％
麦芽糊精                            15％
丙氨酸                              1.5％
缬氨酸                              1.5％
苏氨酸                              1.5％
维生素B1                            0.5％组成，配方中>0；
配方中多联双歧杆菌活菌干粉由冻干的短双歧杆菌、婴儿双歧杆菌、长双歧杆菌和乳杆菌四联菌组成，含菌比例为1:1:1:1；
多联双歧杆菌活菌干粉中设定短双歧杆菌干粉活菌数为2.3×109cfu/g，婴儿双歧杆菌干粉活菌数为1.3×109cfu/g，长双歧杆菌干粉活菌数为2.03×1012cfu/g，乳杆菌干粉活菌数为1.63×108cfu/g。
2、根据权利要求1所述的一种制备方法，其特征在于：多联活性双歧杆菌产品制备时必须在洁净度为100级的环境条件中操作，活菌数为1.0×108cfu/g。
CNB2005100355847A 2005-07-05 2005-07-05 多联活性双歧杆菌产品的制备方法 Active CN100490826C (zh)

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EP2481299B1 (en) * 2011-01-31 2016-12-07 Dr. Fischer Gesundheitsprodukte GmbH Bifidobacterium bifidum strains for application in gastrointestinal diseases
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