CN108359646A - 使植物具有除草剂抗性的ACCase突变型蛋白及其应用 - Google Patents

使植物具有除草剂抗性的ACCase突变型蛋白及其应用 Download PDF

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CN108359646A
CN108359646A CN201810030101.1A CN201810030101A CN108359646A CN 108359646 A CN108359646 A CN 108359646A CN 201810030101 A CN201810030101 A CN 201810030101A CN 108359646 A CN108359646 A CN 108359646A
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张保龙
王金彦
凌溪铁
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Jiangsu Academy of Agricultural Sciences
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Abstract

本发明公开了一种水稻ACCase突变型蛋白，所述ACCase突变型蛋白的氨基酸序列存在以下任意一种或几种突变：其对应于水稻ACCase的氨基酸序列的第1792位、第2015位、第2038位、第2052位、第2089位发生突变。本发明还公开了核酸或基因，其编码所述的蛋白。本发明表达该突变蛋白的植物可以抗（耐）乙酰辅酶A羧化酶类除草剂。本发明的水稻ACCase蛋白的水稻三叶期幼苗在施用3mL喹禾灵/L水（2倍推荐使用浓度）后，植株仍然正常生长发育和结实。

Description

使植物具有除草剂抗性的ACCase突变型蛋白及其应用
技术领域
本发明属于植物蛋白和植物抗除草剂领域，涉及使植物具有除草剂抗性的ACCase突变型蛋白及其应用；具体而言，本发明涉及水稻的乙酰辅酶A羧化酶(ACCase)突变蛋白，该突变蛋白能赋予植物尤其水稻抗乙酰辅酶A羧化酶抑制剂类除草剂的特性。本发明公开了该突变蛋白的核苷酸序列和氨基酸序列，以及它们在植物抗除草剂领域中的应用。
背景技术
农田杂草是导致农作物减产的最主要原因之一。与传统依靠栽培措施、人工除草和机械除草等方法相比，化学除草剂的使用是人们公认的防除农田杂草最高效的、简便的和经济的治理方法。
乙酰辅酶A羧化酶(Acetyl-CoA Carboxylase，简称ACCase)是植物代谢过程中催化植物脂肪酸合成的关键酶，是1958年发现的除草剂的一个作用靶标。ACCase属于生物素包含酶(Biotincontaining enzyme)的类型I(typeI)，存在于大多数的生物体中，主要包含细菌、酵母、真菌、植物、动物和人类中。目前已经确定在植物体内有两种同工型的ACCase，主要位于质体和细胞溶胶中，它们的功能区域在结构上有很大的差别。大多数植物质体中的ACCase有4个亚基：一个生物素羧基载体蛋白亚基(biotincarboxylcarrier-protein，BCCP)，一个生物素羧化酶亚基(biotincarboxylase，BC)，羧基转移酶(carboxyltransferase，CT)的两个亚基CTα和CTβ，二者之间以共价键相连。大多数植物细胞溶胶中的ACCase于酵母、大鼠和人的属于同一类型，它的一条多肽链包含着原核型ACCase所有的4个亚基，排列顺序是BC、BCCP、CTβ、CTα，形成了3个功能域(CTβ和CTα算一个功能域)。除草剂对ACCase的抑制主要是通过抑制CT，造成酰基脂类生物合成受到抑制，影响细胞膜渗透性，造成代谢产物渗漏，致使植物死亡，从而达到防除禾本科杂草的目的。
ACCase类除草剂包括芳氧苯氧丙酸酯类(Aryloxyphenoxypropanoates，APP)、肟醚类环己二酮(Cyclohexanedione oximes，CHD)和苯基吡唑啉类(Phenylpyrazoline,DEN)三类。由于其选择性高，能够苗后防除一年或多年生禾本科杂草，且在植物体内传导，具有高效、低毒、施用期长、对后茬作物安全等优点，此类除草剂迅速发展、品种不断增多、使用面积迅速扩大，已成为世界第三类重要除草剂。
ACCase催化乙酰-CoA生成丙二酰-CoA，该反应是脂肪酸全合成的第一个关键步骤，也是限速步骤，这步由ACCase催化的反应实际上是由3个亚基组成的两步反应。第一步，首先由ATP提供能量给重碳酸，产生活化的羧基(羧酸、磷酸复合物)，然后在BC作用下将羧基连接到BCCP中生物素的脲环N1上，形成羧化的生物素羧基载体蛋白，该过程中由Mg2+或者Mn2+等的参与，碳酸氢盐为羧基供体。第二步，在CT作用下，将生物素脲环N1上的羧基转移至乙酰-CoA的甲基，产生丙二酰-CoA，该过程不需要其他的辅助能量。合成的丙二酰-CoA参与脂肪酸从头合成等生物过程。
ACCase有两类主要的抑制剂类型，包括芳氧笨氧丙酸酯类(APP)和环己二酮类(CHD)，已开发出多个除草剂品种，如禾草灵、盖草能、炔草酯、本草酮、烯草酮等等。早在20世纪60-70年代，APP类和CHD类除草剂就被广泛用于农田禾本科杂草，但是随着长期和大面积的使用，杂草很快对这两种除草剂产生了抗性，目前，已在26个国家发现了高达35种杂草对这两类抑制剂产生了抗性。
但目前，ACCase抑制剂类除草剂的作用机理尚未确定，很难***ACCase蛋白其它氨基酸位点的突变是否会产生除草剂抗性，只能依赖于科研人员长期、艰苦的实践探索，并凭借一些运气才可能发现ACCase蛋白新的除草剂抗性位点。
发明内容
发明目的：本发明所要解决的技术问题是提供一种水稻ACCase突变型蛋白。
本发明还要解决的技术问题是提供了编码水稻ACCase突变型蛋白的核酸或基因。
本发明还要解决的技术问题是提供了含有上述的水稻ACCase突变型基因的表达盒、重组载体或细胞。
本发明还要解决的技术问题是提供了获得具有除草剂抗性植物的方法。具体地，用于增强植物、植物组织、植物细胞对于至少一种除草剂的耐受性的方法，所述除草剂干扰ACCase酶的活性。因此，本文中除草剂抗性指的是抗(耐)乙酰辅酶A羧化酶类除草剂。
本发明所述的增强植物、植物组织或者植物细胞对除草剂的抗性方法可以通过转化或者杂交、自交和无性繁殖的方法实施，也可通过化学诱变或基因定点突变的方式获得，是改变的植株具有本发明的核苷酸序列SEQ ID No:1、SEQ ID No:3、SEQ ID No:5、SEQ IDNo:7或SEQ ID No:9中的任意一种，所述的突变后的植株具有一种或者一种以上的上述突变后的核苷酸序列。
本发明人通过长期、艰苦的研究，对野生型水稻品种镇糯19(从江苏丘陵地区镇江农业科学研究所获得)进行EMS诱变处理，对这些的EMS突变植株进行长期、不断地筛选，发现了一系列ACCase突变蛋白，包括前文所述的某些已知ACCase突变蛋白和新ACCase突变蛋白，其对ACCase抑制剂类除草剂不敏感，从而使得植物具有ACCase抑制剂类除草剂抗性。本发明在植物育种中的应用，可用于培育具有除草剂抗性的植物，尤其是农作物，还开发了这些蛋白及其编码基因在转基因或者非转基因如水稻等作物中的应用。
技术方案：为了解决上述技术问题，本发明采用的技术方案如下：一种水稻ACCase突变型蛋白，所述ACCase突变型蛋白的氨基酸序列存在以下任意一种或几种突变：其对应于水稻ACCase的氨基酸序列的第1792位、第2015位、第2038位、第2052位、第2089位发生突变。
作为优选，本发明在水稻ACCase的氨基酸序列的第1792位氨基酸由异亮氨酸突变为亮氨酸、第2015位基酸由丙氨酸突变为缬氨酸、第2038位氨基酸由色氨酸变为半胱氨酸、第2052位氨基酸由异亮氨酸突变为天冬酰胺、第2089位氨基酸由天冬氨酸变为甘氨酸。
本发明的ACCase突变型蛋白发生突变的氨基酸包括但不仅限于以下几种突变，在以下位点突变成其他氨基酸的情况也在本发明的保护范围之内例如：第1792位氨基酸由异亮氨酸突变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、缬氨酸、丙氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸；第2015位基酸由丙氨酸突变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸；第2038位氨基酸由色氨酸变为苯丙氨酸、丝氨酸、酪氨酸、丙氨酸、脯氨酸、苏氨酸、缬氨酸、异亮氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、赖氨酸、组氨酸、甲硫氨酸、亮氨酸；第2052位氨基酸由异亮氨酸突变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、缬氨酸、天冬氨酸、甘氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、丙氨酸、第2089位氨基酸由天冬氨酸变为苯丙氨酸、丝氨酸、酪氨酸、半胱氨酸、脯氨酸、苏氨酸、缬氨酸、异亮氨酸、精氨酸、谷氨酰胺、色氨酸、赖氨酸、组氨酸、甲硫氨酸、亮氨酸、丙氨酸。
本发明的内容还包括一种水稻ACCase突变型蛋白，包括：
(a)其氨基酸序列如SEQ ID NO.2或SEQ ID NO.4或SEQ ID NO.6或SEQ ID NO.8或SEQ ID NO.10所示；或
(b)在(a)中的氨基酸序列经过取代和/或缺失和/或添加一个或几个氨基酸且具有乙酰辅酶A羧化酶活性的由(a)衍生的蛋白质。
本发明的内容还包括核酸或基因，其编码所述的蛋白。
本发明所述的核酸或基因，包括：
(a)其编码所述的蛋白；或
(b)在严格条件下与(a)限定的核苷酸序列杂交且编码具有乙酰辅酶A羧化酶活性的蛋白质的核苷酸序列；或
(c)其核苷酸序列如SEQ ID NO:1或SEQ ID NO:3或SEQ ID NO:5或SEQ ID NO:7或SEQ ID NO:9所示。
所述严格条件为：在0.1×SSC、0.1％SDS的溶液中，65℃洗膜15分钟的高度严谨的杂交条件。
本发明的ACCase突变型基因发生突变的核苷酸包括但不仅限于以下几种突变，在以下位点突变成其他核苷酸情况也在本发明的保护范围之内例如：A突变为T、C、G；或C突变为T、A、G；或T突变为A、C、G；或G突变为T、C、A等等均在本发明的保护范围之内。
进一步地，本发明的水稻ACCase突变型基因，所述水稻ACCase基因存在以下一种或几种突变：在水稻ACCase基因的第5374位核苷酸、在水稻ACCase基因的第6044位核苷酸、在水稻ACCase基因的第6114位核苷酸、在水稻ACCase基因的第6155位核苷酸、在水稻ACCase基因的第6266位核苷酸发生突变。
作为优选，本发明的水稻ACCase突变型基因，所述水稻ACCase基因存在以下一种或几种突变：在水稻ACCase基因的第5374位核苷酸由A突变为T，在水稻ACCase基因的第6044位核苷酸由C突变为T，在水稻ACCase基因的第6114位核苷酸由G突变为T，在水稻ACCase基因的第6155位核苷酸由T突变为A，在水稻ACCase基因的第6266位核苷酸由A突变为G。
本发明内容还包括表达盒、重组载体或细胞，其含有所述的核酸或基因。
本发明内容还包括所述的水稻ACCase突变型蛋白、核酸或基因，表达盒、重组载体或细胞在植物抗除草剂方面的应用。
其中，上述除草剂是喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或几种。
本发明内容还包括获得具有除草剂抗性植物的方法，包括如下步骤：
1)使植物包含所述的核酸或基因；或
2)使植物表达所述的水稻ACCase突变型蛋白。
所述的方法，其包括crispr基因编辑、转基因、杂交、回交或无性繁殖步骤。
本发明内容还包括鉴定植物的方法，其中植物是包含所述的核酸或基因的植物、表达所述的蛋白的植物或所述的方法获得的植物，包括以下步骤：
1)测定所述植物是否包含所述的核酸或基因；或，
2)测定所述植物是否表达所述的蛋白。
本发明内容还包括一种除草剂的防护剂，该防护剂由所述的水稻ACCase突变型蛋白制成。
本发明内容还包括一种控制杂草的方法，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含所述的核酸或基因或所述的表达盒、重组载体或细胞，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或几种。
本发明内容还包括一种用于保护植物免受由除草剂引起的损伤的方法，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含所述的核酸或基因或所述的表达盒、重组载体导入植物，导入后的植物产生除草剂抗性蛋白，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或几种。
进一步地，本发明的野生型基因来源于水稻的镇糯19。
进一步地，本发明的镇糯19的突变植株具有喹禾灵除草剂抗性，本发明的镇糯19突变植株的突变型基因的核苷酸序列或者氨基酸序列在植物中表达时能赋予水稻植物增加对乙酰辅酶A羧化酶类除草剂的耐受性。
进一步地，本发明通过对镇糯19进行突变分别获得了具备除草剂抗性的水稻植株ACCase-5374突变体、ACCase-6044突变体、ACCase-6114突变体、ACCase-6155突变体、ACCase-6266突变体，该五个植株分别保藏于中国典型培养物保藏中心(CCTCC)。
其中，本发明的镇糯19的突变型基因序列的编号及其对应的核苷酸和氨基酸序列分别是：将在镇糯19的ACCase基因的第5374位核苷酸由A突变为T的突变体命名为ACCase-5374突变体，ACCase-5374突变体的ACCase基因其核苷酸序列如SEQ ID No:1所示，其氨基酸序列如SEQ ID No:2所示；
其中，将在镇糯19的ACCase基因的第6044位核苷酸由C突变为T的突变体命名为ACCase-6044突变体，ACCase-6044突变体的ACCase基因其核苷酸序列如SEQ ID No:3所示，其氨基酸序列如SEQ ID No:4所示；
其中，将在镇糯19的水稻ACCase基因的第6114位核苷酸由G突变为T的突变体命名为ACCase-6114突变体，ACCase-6114突变体ACCase基因其核苷酸序列如SEQ ID No:5所示，其氨基酸序列如SEQ ID No:6所示；
其中，将在镇糯19的水稻ACCase基因的第6155位核苷酸由T突变为A的突变体命名为ACCase-6155突变体，ACCase-6155突变体ACCase基因其核苷酸序列如SEQ ID No:7所示，其氨基酸序列如SEQ ID No:8所示；
其中，将在镇糯19的水稻ACCase基因的第6266位核苷酸由A突变为G的突变体命名为，ACCase-6266突变体，ACCase-6266突变体的ACCase基因其核苷酸序列如SEQ ID No:9所示，其氨基酸序列如SEQ ID No:10所示；
其中，镇糯19野生型ACCase基因核苷酸序列如SEQ ID No:11所示，其氨基酸序列如SEQ ID No:12所示。
有益效果：相对于现有技术，本发明具备以下优点：
1)本发明首次通过随机突变的方式获得了具备除草剂抗性的水稻植株ACCase-5374突变体、ACCase-6044突变体、ACCase-6114突变体、ACCase-6155突变体、ACCase-6266突变体，该植株分别保藏于中国典型培养物保藏中心(CCTCC)。
2)在田间喷施ACCase抑制剂类除草剂喹禾灵的实验结果表明，含有本发明的水稻ACCase突变蛋白的水稻三叶期幼苗在施用2倍推荐使用浓度的乙酰辅酶A羧化酶抑制剂类除草剂后，植株仍然正常生长发育和结实，而野生型水稻幼苗在施用1倍推荐使用浓度的乙酰辅酶A羧化酶抑制剂类除草剂后，植株生长逐渐停止，叶片逐渐失绿、变浅黄色，植株不能拔节，最终整株死亡。
附图说明
图1喹禾灵除草剂筛选获得的抗性水稻突变体；
图2PCR扩增5个水稻抗除草剂突变体的ACCase基因全长结果图；第1泳道为Marker；Marker分子量从上到下依次为15kb、8kb、5kb、3kb、1kb、1.5kb、1kb、750bp、500bp，第2泳道为野生型镇糯19的DNA，第3泳道为抗除草剂突变植株(ACCase-5374突变体)的DNA，第4泳道为抗除草剂突变植株(ACCase-6044突变体)的DNA，第5泳道为抗除草剂突变植株(ACCase-6114突变体)的DNA，第6泳道为抗除草剂突变植株(ACCase-6155突变体)的DNA，第7泳道为抗除草剂突变植株(ACCase-6266突变体)的DNA。
具体实施方式
下面将结合实施例对本发明的实施方案进行详细描述，但是本领域技术人员将会理解，下列实施例仅用于说明本发明，而不应视为限定本发明的范围。实施例中未注明具体条件者，按照常规条件或制造商建议的条件进行。所用试剂或仪器未注明生产厂商者，均为可以通过市购获得的常规产品。
实施例1：水稻抗喹禾灵除草剂突变体获取过程
将镇糯19号(江苏丘陵地区镇江农业科学研究所惠赠)(此为M0，用清水浸泡2小时)120kg分6次用0.4-0.6％(w/w)甲磺酸乙酯(EMS)室温下浸泡6-9小时，期间每1小时摇动一次种子；弃去EMS溶液，自来水翻动浸泡种子5次，每次5分钟，然后用自来水冲洗种子过夜，次日进行田间播种，并进行常规肥水管理(此为M1)。植株成熟后，种子混收、晾干，过冬保存。次年播种田间。待水稻(此为M2)幼苗长至三叶期时，喷施为3mL喹禾灵/L水(推荐最低使用浓度为1.5mL喹禾灵/1L水)，1个月后还呈正常绿色、且能正常生长的植株即为抗喹禾灵除草剂的水稻突变植株(图1)。共计获得抗除草剂的M2单株28株，这些抗性单株进行常规肥水管理，均可正常结实，种子成熟后，单株收种、晾干，过冬保存。我们对上述获得的28株M2进行单株收获，并进行种植M3种子。
实施例2：水稻镇糯19号野生型Accase全长基因的获得
选取镇糯19号野生型植株的叶片，提取基因组DNA，根据NCBI中日本晴ACCase基因(NCBI：XM_015783727)设计引物进行扩增，采用KOD DNA polymerase聚合酶(购自Toyobo公司)扩增ACCase基因，其反应体系如下：
10×Buffer5.0μl
10μM正向引物(GTCAGATTTCACACATCTGGGGAT)1.0μl
10μM反向引物(ACTTGCACTTTCATCTGGCAGAAC)1.0μl
20-30ng/μL水稻镇糯19号基因组DNA 1.0μl
2mMdNTP 2.0μl
25mM MgSO42.0μl
KOD DNApolymerase聚合酶(1U/μl)1.0μl
补加无菌水至总体积50μl
PCR扩增反应程序采用两步法，退火和延伸合为一起，采用68度。
程序如下：预变性：94℃3min；35个循环：变性94℃10sec；延伸68℃10min；保温：72℃10min。
取2μl PCR产物经1％琼脂糖凝胶电泳检测，发现有预期大小的片段后(图2)，剩余的PCR产物经PCR清洁试剂盒(购自Axygen公司)清洁回收后，克隆至pMD19-T载体(购自Takara公司)，然后转化大肠杆菌。每个转化随机挑取12个大肠杆菌单克隆进行PCR检测，取PCR结果呈阳性的6个单克隆，送南京一道生物科技有限公司测序，获得突变ACCase基因序列，该基因片段长度为13127bp，通过去除内含子后获得基因全长6984bp，镇糯19野生型ACCase序列全长基因片段的测序结果如下：
ATGACATCCACACATGTGGCGACATTGGGAGTTGGTGCCCAGGCACCTCCTCGTCACCAGAAAAAGTCAGCTGGCACTGCATTTGTATCATCTGGGTCATCAAGACCCTCATACCGAAAGAATGGTCAGCGTACTCGGTCACTTAGGGAAGAAAGCAATGGAGGAGTGTCTGATTCCAAAAAGCTTAACCACTCTATTCGCCAAGGTCTTGCTGGCATCATTGACCTCCCAAATGACGCAGCTTCAGAAGTTGATATTTCACATGGTTCCGAAGATCCCAGGGGGCCTACGGTCCCAGGTTCCTACCAAATGAATGGGATTATCAATGAAACACATAATGGGAGGCATGCTTCAGTCTCCAAGGTTGTTGAGTTTTGTACGGCACTTGGTGGCAAAACACCAATTCACAGTGTATTAGTGGCCAACAATGGAATGGCAGCAGCTAAGTTCATGCGGAGTGTCCGAACATGGGCTAATGATACTTTTGGATCAGAGAAGGCAATTCAGCTGATAGCTATGGCAACTCCGGAGGATCTGAGGATAAATGCAGAGCACATCAGAATTGCCGATCAATTTGTAGAGGTACCTGGTGGAACAAACAACAACAACTATGCAAATGTCCAACTCATAGTGGAGATAGCAGAGAGAACAGGTGTTTCTGCTGTTTGGCCTGGTTGGGGTCATGCATCTGAGAATCCTGAACTTCCAGATGCGCTGACTGCAAAAGGAATTGTTTTTCTTGGGCCACCAGCATCATCAATGCATGCATTAGGAGACAAGGTTGGCTCAGCTCTCATTGCTCAAGCAGCTGGAGTTCCAACACTTGCTTGGAGTGGATCACATGTGGAAGTTCCTCTGGAGTGTTGCTTGGACTCAATACCTGATGAGATGTATAGAAAAGCTTGTGTTACTACCACAGAGGAAGCAGTTGCAAGTTGTCAGGTGGTTGGTTATCCTGCCATGATTAAGGCATCTTGGGGTGGTGGTGGTAAAGGAATAAGGAAGGTTCATAATGATGATGAGGTTAGGACATTATTTAAGCAAGTTCAAGGCGAAGTACCTGGTTCCCCAATATTTATCATGAGGCTAGCTGCTCAGAGTCGACATCTTGAAGTTCAGTTGCTTTGTGATCAATATGGCAACGTAGCAGCACTTCACAGTCGAGATTGCAGTGTACAACGGCGACACCAAAAGATAATCGAGGAAGGACCAGTTACTGTTGCTCCTCGTGAGACTGTGAAAGAGCTTGAGCAGGCAGCACGGAGGCTTGCTAAAGCTGTGGGTTATGTTGGTGCTGCTACTGTTGAATACCTTTACAGCATGGAAACTGGTGAATATTATTTTCTGGAACTTAATCCACGGCTACAGGTTGAGCATCCTGTCACTGAGTGGATAGCTGAAGTAAATTTGCCTGCGGCTCAAGTTGCTGTTGGAATGGGTATACCCCTTTGGCAGATTCCAGAGATCAGGCGCTTCTACGGAATGAACCATGGAGGAGGCTATGACCTTTGGAGGAAAACAGCAGCTCTAGCGACTCCATTTAACTTTGATGAAGTAGATTCTAAATGGCCAAAAGGCCACTGCGTAGCTGTTAGAATAACTAGCGAGGATCCAGATGATGGGTTTAAGCCTACTGGTGGAAAAGTAAAGGAGATAAGTTTCAAGAGTAAACCAAATGTTTGGGCCTATTTCTCAGTAAAGTCTGGTGGAGGCATCCATGAATTCGCTGATTCTCAGTTCGGACATGTTTTTGCGTATGGAACTACTAGATCGGCAGCAATAACTACCATGGCTCTTGCACTAAAAGAGGTTCAAATTCGTGGAGAAATTCATTCAAACGTAGACTACACAGTTGACCTATTAAATGCCTCAGATTTTAGAGAAAATAAGATTCATACTGGTTGGCTGGATACCAGGATAGCCATGCGTGTTCAAGCTGAGAGGCCTCCATGGTATATTTCAGTCGTTGGAGGGGCTTTATATAAAACAGTAACTGCCAACACGGCCACTGTTTCTGATTATGTTGGTTATCTTACCAAGGGCCAGATTCCACCAAAGCATATATCCCTTGTCTATACGACTGTTGCTTTGAATATAGATGGGAAAAAATATACAATCGATACTGTGAGGAGTGGACATGGTAGCTACAGATTGCGAATGAATGGATCAACGGTTGACGCAAATGTACAAATATTATGTGATGGTGGGCTTTTAATGCAGCTGGATGGAAACAGCCATGTAATTTATGCTGAAGAAGAGGCCAGTGGTACACGACTTCTTATTGATGGAAAGACATGCATGTTACAGAATGACCATGACCCATCAAAGTTATTAGCTGAGACACCATGCAAACTTCTTCGTTTCTTGGTTGCTGATGGTGCTCATGTTGATGCTGATGTACCATATGCGGAAGTTGAGGTTATGAAGATGTGCATGCCCCTCTTATCACCCGCTTCTGGTGTCATACATGTTGTAATGTCTGAGGGCCAAGCAATGCAGGCTGGTGATCTTATAGCTAGGCTGGATCTTGATGACCCTTCTGCTGTTAAGAGAGCTGAGCCGTTCGAAGATACTTTTCCACAAATGGGTCTCCCTATTGCTGCTTCTGGCCAAGTTCACAAATTATGTGCTGCAAGTCTGAATGCTTGTCGAATGATCCTTGCGGGGTATGAGCATGATATTGACAAGGTTGTGCCAGAGTTGGTATACTGCCTAGACACTCCGGAGCTTCCTTTCCTGCAGTGGGAGGAGCTTATGTCTGTTTTAGCAACTAGACTTCCAAGAAATCTTAAAAGTGAGTTGGAGGGCAAATATGAGGAATACAAAGTAAAATTTGACTCTGGGATAATCAATGATTTCCCTGCCAATATGCTACGAGTGATAATTGAGGAAAATCTTGCATGTGGTTCTGAGAAGGAGAAGGCTACAAATGAGAGGCTTGTTGAGCCTCTTATGAGCCTACTGAAGTCATATGAGGGTGGGAGAGAAAGTCATGCTCACTTTGTTGTCAAGTCCCTTTTTGAGGAGTATCTCTATGTTGAAGAATTGTTCAGTGATGGAATTCAGTCTGATGTGATTGAGCGTCTGCGCCTTCAACATAGTAAAGACCTACAGAAGGTCGTAGACATTGTGTTGTCCCACCAGAGTGTTAGAAATAAAACTAAGCTGATACTAAAACTCATGGAGAGTCTGGTCTATCCAAATCCTGCTGCCTACAGGGATCAATTGATTCGCTTTTCTTCCCTTAATCACAAAGCGTATTACAAGTTGGCACTTAAAGCTAGTGAACTTCTTGAACAAACAAAACTTAGTGAGCTCCGTGCAAGAATAGCAAGGAGCCTTTCAGAGCTGGAGATGTTTACTGAGGAAAGCAAGGGTCTCTCCATGCATAAGCGAGAAATTGCCATTAAGGAGAGCATGGAAGATTTAGTCACTGCTCCACTGCCAGTTGAAGATGCGCTCATTTCTTTATTTGATTGTAGTGATACAACTGTTCAACAGAGAGTGATTGAGACTTATATAGCTCGATTATACCAGCCTCATCTTGTAAAGGACAGTATCAAAATGAAATGGATAGAATCGGGTGTTATTGCTTTATGGGAATTTCCTGAAGGGCATTTTGATGCAAGAAATGGAGGAGCGGTTCTTGGTGACAAAAGATGGGGTGCCATGGTCATTGTCAAGTCTCTTGAATCACTTTCAATGGCCATTAGATTTGCACTAAAGGAGACATCACACTACACTAGCTCTGAGGGCAATATGATGCATATTGCTTTGTTGGGTGCTGATAATAAGATGCATATAATTCAAGAAAGTGGTGATGATGCTGACAGAATAGCCAAACTTCCCTTGATACTAAAGGATAATGTAACCGATCTGCATGCCTCTGGTGTGAAAACAATAAGTTTCATTGTTCAAAGAGATGAAGCACGGATGACAATGCGTCGTACCTTCCTTTGGTCTGATGAAAAGCTTTCTTATGAGGAAGAGCCAATTCTCCGGCATGTGGAACCTCCTCTTTCTGCACTTCTTGAGTTGGACAAGTTGAAAGTGAAAGGATACAATGAAATGAAGTATACCCCATCACGGGATCGTCAATGGCATATCTACACACTTAGAAATACTGAAAACCCCAAAATGTTGCACCGGGTATTTTTCCGAACCCTTGTCAGGCAACCCAGTGTATCCAACAAGTTTTCTTCGGGCCAGATTGGTGACATGGAAGTTGGGAGTGCTGAAGAACCTCTGTCATTTACATCAACCAGCATATTAAGATCTTTGATGACTGCTATAGAGGAATTGGAGCTTCACGCAATTAGAACTGGCCATTCACACATGTATTTGCATGTATTGAAAGAACAAAAGCTTCTTGATCTTGTTCCAGTTTCAGGGAATACAGTTTTGGATGTTGGTCAAGATGAAGCTACTGCATATTCACTTTTAAAAGAAATGGCTATGAAGATACATGAACTTGTTGGTGCAAGAATGCACCATCTTTCTGTATGCCAATGGGAAGTGAAACTTAAGTTGGACTGCGATGGTCCTGCCAGTGGTACCTGGAGGATTGTAACAACCAATGTTACTAGTCACACTTGCACTGTGGATATCTACCGTGAGATGGAAGATAAAGAATCACGGAAGTTAGTATACCATCCCGCCACTCCGGCGGCTGGTCCTCTGCATGGTGTGGCACTGAATAATCCATATCAGCCTTTGAGTGTCATTGATCTCAAACGCTGTTCTGCTAGGAATAATAGAACTACATACTGCTATGATTTTCCACTGGCATTTGAAACTGCAGTGAGGAAGTCATGGTCCTCTAGTACCTCTGGTGCTTCTAAAGGTGTTGAAAATGCCCAATGTTATGTTAAAGCTACAGAGTTGGTATTTGCGGACAAACATGGGTCATGGGGCACTCCTTTAGTTCAAATGGACCGGCCTGCTGGGCTCAATGACATTGGTATGGTAGCTTGGACCTTGAAGATGTCCACTCCTGAATTTCCTAGTGGTAGGGAGATTATTGTTGTTGCAAATGATATTACGTTCAGAGCTGGATCATTTGGCCCAAGGGAAGATGCATTTTTTGAAGCTGTTACCAACCTAGCCTGTGAGAAGAAACTTCCTCTTATTTATTTGGCAGCAAATTCTGGTGCTCGAATTGGCATAGCAGATGAAGTGAAATCTTGCTTCCGTGTTGGGTGGTCTGATGATGGCAGCCCTGAACGTGGGTTTCAGTACATTTATCTAAGCGAAGAAGACTATGCTCGTATTGGCACTTCTGTCATAGCACATAAGATGCAGCTAGACAGTGGTGAAATTAGGTGGGTTATTGATTCTGTTGTGGGCAAGGAAGATGGACTTGGTGTGGAGAATATACATGGAAGTGCTGCTATTGCCAGTGCTTATTCTAGGGCATATAAGGAGACATTTACACTTACATTTGTGACTGGAAGAACTGTTGGAATAGGAGCTTATCTTGCTCGACTTGGCATCCGGTGCATACAGCGTCTTGACCAGCCTATTATTCTTACAGGCTATTCTGCACTGAACAAGCTTCTTGGGCGGGAAGTGTACAGCTCCCACATGCAGTTGGGTGGTCCCAAAATCATGGCAACTAATGGTGTTGTCCATCTTACTGTTTCAGATGACCTTGAAGGCGTTTCTAATATATTGAGGTGGCTCAGTTATGTTCCTGCCTACATTGGTGGACCACTTCCAGTAACAACACCGTTGGACCCACCGGACAGACCTGTTGCATACATTCCTGAGAACTCGTGTGATCCTCGAGCGGCTATCCGTGGTGTTGATGACAGCCAAGGGAAATGGTTAGGTGGTATGTTTGATAAAGACAGCTTTGTGGAAACATTTGAAGGTTGGGCTAAGACAGTGGTTACTGGCAGAGCAAAGCTTGGTGGAATTCCAGTGGGTGTGATAGCTGTGGAGACTCAGACCATGATGCAAACTATCCCTGCTGACCCTGGTCAGCTTGATTCCCGTGAGCAATCTGTTCCTCGTGCTGGACAAGTGTGGTTTCCAGATTCTGCAACCAAGACTGCGCAGGCATTGCTGGACTTCAACCGTGAAGGATTACCTCTGTTCATCCTCGCTAACTGGAGAGGCTTCTCTGGTGGACAAAGAGATCTTTTTGAAGGAATTCTTCAGGCTGGCTCGACTATTGTTGAGAACCTTAGGACATACAATCAGCCTGCCTTTGTCTACATTCCCATGGCTGCAGAGCTACGAGGAGGGGCTTGGGTTGTGGTTGATAGCAAGATAAACCCAGACCGCATTGAGTGCTATGCTGAGAGGACTGCAAAAGGCAATGTTCTGGAACCGCAAGGGTTAATTGAGATCAAGTTCAGGTCAGAGGAACTCCAGGATTGCATGAGTCGGCTTGACCCAACATTAATTGATCTGAAAGCAAAACTCGAAGTAGCAAATAAAAATGGAAGTGCTGACACAAAATCGCTTCAAGAAAATATAGAAGCTCGAACAAAACAGTTGATGCCTCTATATACTCAGATTGCGATACGGTTTGCTGAATTGCATGATACATCCCTCAGAATGGCTGCGAAAGGTGTGATTAAGAAAGTTGTGGACTGGGAAGAATCACGATCTTTCTTCTATAAGAGATTACGGAGGAGGATCTCTGAGGATGTTCTTGCAAAAGAAATTAGAGCTGTAGCAGGTGAGCAGTTTTCCCACCAACCAGCAATCGAGCTGATCAAGAAATGGTATTCAGCTTCACATGCAGCTGAATGGGATGATGACGATGCTTTTGTTGCTTGGATGGATAACCCTGAAAACTACAAGGATTATATTCAATATCTTAAGGCTCAAAGAGTATCCCAATCCCTCTCAAGTCTTTCAGATTCCAGCTCAGATTTGCAAGCCCTGCCACAGGGTCTTTCCATGTTACTAGATAAGATGGATCCCTCTAGAAGAGCTCAACTTGTTGAAGAAATCAGGAAGGTCCTTGGTTGA
实施例3：抗喹禾灵除草剂水稻突变体分子水平鉴定
上述实施例1获得的所有抗除草剂水稻突变植株中，选取多个突变植株的叶片和镇糯19号野生型植株的叶片，分别提取基因组DNA，并用ACCase-F(GTCAGATTTCACACATCTGGGGAT)和ACCase-R(ACTTGCACTTTCATCTGGCAGAAC)分别进行PCR扩增。PCR扩增条件与扩增体系同实施例2，测序结果发现：与实施例2的野生型植株相比，我们总共获得了五个水稻突变体，上述抗除草剂的水稻突变体在ACCase基因序列上都发生突变。具体的突变内容如下：
ACCase-5374突变体的ACCase基因序列，其核苷酸序列的第5374位核苷酸由A突变为T，导致其编码的氨基酸序列的第1792位氨基酸由异亮氨酸突变为亮氨酸，其核苷酸序列如SEQ ID No:1所示，其编码的氨基酸序列如SEQ ID No:2所示；
ACCase-6044突变体的ACCase基因序列，其核苷酸序列的第6044位核苷酸由C突变为T，导致其编码的氨基酸序列的第2015位氨基酸由丙氨酸变为缬氨酸，其核苷酸序列如SEQ ID No:3所示，其编码的氨基酸序列如SEQ ID No:4所示；
ACCase-6114突变体的ACCase基因序列，其核苷酸序列的第6114位核苷酸由G突变为T，导致其编码的氨基酸序列的第2038位氨基酸由色氨酸变为半胱氨酸，其核苷酸序列如SEQ ID No:5所示，其编码的氨基酸序列如SEQ ID No:6所示；
ACCase-6155突变体的ACCase基因序列，其核苷酸序列第6155位由T突变为A，导致其编码的氨基酸序列的第2052位氨基酸由异亮氨酸突变为天冬酰胺，其核苷酸序列如SEQID No:7所示，其编码的氨基酸序列如SEQ ID No:8所示；
ACCase-6266突变体的ACCase基因序列，其核苷酸序列第6266位由A突变为G，导致其编码的氨基酸序列第2089位氨基酸由天冬氨酸变为甘氨酸，其核苷酸序列如SEQID No:9所示，其编码的氨基酸序列如SEQ ID No:10所示。
本发明的ACCase-5374突变体其分类命名为水稻种子zhen19-21(Oryza sativaJaponica Group zhen19-21)，该材料已于2017年12月20日保藏于中国典型培养物保藏中心(CCTCC)，地址：湖北省武汉市武昌区武汉大学保藏中心(武汉大学第一附属小学对面)，邮编：430072，保藏编号为CCTCC No：P201803。
本发明的ACCase-6044突变体其分类命名为水稻种子zhen19-60(Oryza sativaJaponica Group zhen19-60)，该材料已于2017年12月20日保藏于中国典型培养物保藏中心(CCTCC)，地址：湖北省武汉市武昌区武汉大学保藏中心(武汉大学第一附属小学对面)，邮编：430072，保藏编号为CCTCC No：P201804。
本发明的ACCase-6114突变体其分类命名为水稻种子zhen19-26(Oryza sativaJaponica Group zhen19-26)，该材料已于2017年12月20日保藏于中国典型培养物保藏中心(CCTCC)，地址：湖北省武汉市武昌区武汉大学保藏中心(武汉大学第一附属小学对面)，邮编：430072，保藏编号为CCTCC No：P201805。
本发明的ACCase-6155突变体其分类命名为水稻种子zhen19-38(Oryza sativaJaponica Group zhen19-38)，该材料已于2017年12月20日保藏于中国典型培养物保藏中心(CCTCC)，地址：湖北省武汉市武昌区武汉大学保藏中心(武汉大学第一附属小学对面)，邮编：430072，保藏编号为CCTCC No：P201806。
本发明的ACCase-6266突变体其分类命名为水稻种子zhen19-7(Oryza sativaJaponica Group zhen19-7)，该材料已于2017年12月20日保藏于中国典型培养物保藏中心(CCTCC)，地址：湖北省武汉市武昌区武汉大学保藏中心(武汉大学第一附属小学对面)，邮编：430072，保藏编号为CCTCC No：P201807。
实施例4抗喹禾灵除草剂水稻突变体后代乙酰辅酶A羧化酶抑制剂类除草剂的抗性
将实施例3中鉴定为纯合突变体的五种突变体水稻株系收种，并播种，待水稻长至三叶期时，分别喷施乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵(喷施浓度为施用1.5mL喹禾灵/L水(1倍推荐使用浓度))、吡氟氯禾灵(喷施浓度为施用1.5mL吡氟氯禾灵/L水(1倍推荐使用浓度))、烯草酮(喷施浓度为施用1.5mL烯草酮/L水(1倍推荐使用浓度))、吡氟禾草灵(喷施浓度为施用1.5mL吡氟禾草灵/L水(1倍推荐使用浓度))，喷施一个月后调查枯死情况，发现野生型对照在喷施和以上同样浓度的乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵后，几乎停止生长，新长出来的叶片完全枯黄，并逐渐枯死。而我们筛选到的不同位点的抗除草剂突变体阳性苗则全部对乙酰辅酶A 羧化酶抑制剂类除草剂表现出抗的表型，其正常的生长发育几乎没有受到影响，最终正常拔节抽穗。表1中显示本发明五种突变体水稻对ACCase抑制性除草剂的抗性比野生型要高，伤害百分比要低60％以上。这表明我们筛选到的抗除草剂突变体确实能够稳定遗传的获得抗乙酰辅酶A羧化酶抑制剂类除草剂的抗性。
表1乙酰辅酶A羧化酶类除草剂对水稻野生型和突变体的伤害比率
同时，我们将鉴定为纯合突变体的五种突变体水稻株系收种，并播种，待水稻长至三叶期时，喷施乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵(喷施浓度为施用3mL喹禾灵/L水(2倍推荐使用浓度))、吡氟氯禾灵(喷施浓度为3mL吡氟氯禾灵/L水(2倍推荐使用浓度))、烯草酮(喷施浓度为3mL烯草酮/L水(2倍推荐使用浓度))、吡氟禾草灵(喷施浓度3m吡氟禾草灵/L水(2倍推荐使用浓度))，植株仍然正常生长发育和结实，而野生型水稻幼苗在施用乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵(喷施浓度为施用1.5mL喹禾灵/L水(1倍推荐使用浓度))、吡氟氯禾灵(喷施浓度为1.5mL吡氟氯禾灵/L水(1倍推荐使用浓度))、烯草酮(喷施浓度为1.5mL烯草酮/L水(1倍推荐使用浓度))、吡氟禾草灵(喷施浓度1.5mL吡氟禾草灵/L水(1倍推荐使用浓度))，后，植株生长逐渐停止，叶片逐渐失绿、变浅黄色，植株不能拔节，最终整株死亡。
实施例5抗性材料的两两杂交
为了检测是否具有双重突变位点的抗性植株会比具有单位点抗性植株的除草剂耐受能力更强，我们将实施例3中获得的具有不同突变位点的抗性植株进行两两杂交。选择ACCase-5374突变体株系M021(序列对应SEQ ID No:1)、ACCase-6044突变体株系M098(序列对应SEQ ID No:3)和ACCase-6144突变体株系M138(序列对应SEQ ID No:5)，将这三个株系在温室中种植，开花期将M021与M098、M021与M138、M098与M138分别进行杂交。具体方法是：将母本人工去雄并套袋，父本植株开花的时候，将父本花药中的花粉抖出，授粉到母本柱头上，重复两次，带种子成熟后收种，为F1带杂交种。收获的F1代杂交种繁殖一代，收获F2代群体，M021与M098杂交F2代群体中鉴定带有纯合的ACCase-5374和ACCase-6044突变的植株；M021和M138杂交的F2群体中鉴定带有纯合ACCase-5374和ACCase-6144突变的植株；M098和M138杂交的F2群体中鉴定带有纯合ACCase6044和ACCase-6144突变的植株。待以上杂交水稻植株长至三叶期时，喷施乙酰辅酶A羧化酶抑制剂类除草剂喹禾灵(喷施浓度为施用1.5mL喹禾灵/L水(1倍推荐使用浓度))、吡氟氯禾灵(喷施浓度为施用1.5mL吡氟氯禾灵/L水(1倍推荐使用浓度))、烯草酮(喷施浓度为施用1.5mL烯草酮/L水(1倍推荐使用浓度))、吡氟禾草灵(喷施浓度为施用1.5mL吡氟禾草灵/L水(1倍推荐使用浓度))，结果表明，同时突变两个位点的植株相对于只有一个突变位点的植株，对除草剂的抗性得到了明显的提高(表2)。
表2乙酰辅酶A羧化酶类除草剂对水稻野生型和突变体的伤害比率
尽管本发明的具体实施方式已经得到详细描述，本领域技术人员将会理解。根据已经公开的所有教导，可以对那些细节进行各种修改和替换，这些均在本发明的保护范围内。本发明的全部范围由所附专利要求极其任何等同物给出。
序列表
<110> 江苏省农业科学院
<120> 使植物具有除草剂抗性的ACCase突变型蛋白及其应用
<160> 14
<170> SIPOSequenceListing 1.0
<210> 1
<211> 6984
<212> DNA
<213> 水稻种子zhen19-21(Oryza sativa Japonica Group zhen19-21)
<400> 1
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag aatttacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 2
<211> 2327
<212> PRT
<213> 水稻种子zhen19-21(Oryza sativa Japonica Group zhen19-21)
<400> 2
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Leu
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 3
<211> 6984
<212> DNA
<213> 水稻种子zhen19-60(Oryza sativa Japonica Group zhen19-60)
<400> 3
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgtaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 4
<211> 2327
<212> PRT
<213> 水稻种子zhen19-60(Oryza sativa Japonica Group zhen19-60)
<400> 4
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Val Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 5
<211> 6984
<212> DNA
<213> 水稻种子zhen19-26(Oryza sativa Japonica Group zhen19-26)
<400> 5
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctgtagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 6
<211> 2327
<212> PRT
<213> 水稻种子zhen19-26(Oryza sativa Japonica Group zhen19-26)
<400> 6
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Cys Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 7
<211> 6984
<212> DNA
<213> 水稻种子zhen19-38(Oryza sativa Japonica Group zhen19-38)
<400> 7
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaaatcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 8
<211> 2327
<212> PRT
<213> 水稻种子zhen19-38(Oryza sativa Japonica Group zhen19-38)
<400> 8
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Asn Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 9
<211> 6984
<212> DNA
<213> 水稻种子zhen19-7(Oryza sativa Japonica Group zhen19-7)
<400> 9
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttggtagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 10
<211> 2327
<212> PRT
<213> 水稻种子zhen19-7(Oryza sativa Japonica Group zhen19-7)
<400> 10
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Gly Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 11
<211> 6984
<212> DNA
<213> 水稻镇糯19野生型基因(Oryza sativa Japonica Group zhen19)
<400> 11
atgacatcca cacatgtggc gacattggga gttggtgccc aggcacctcc tcgtcaccag 60
aaaaagtcag ctggcactgc atttgtatca tctgggtcat caagaccctc ataccgaaag 120
aatggtcagc gtactcggtc acttagggaa gaaagcaatg gaggagtgtc tgattccaaa 180
aagcttaacc actctattcg ccaaggtctt gctggcatca ttgacctccc aaatgacgca 240
gcttcagaag ttgatatttc acatggttcc gaagatccca gggggcctac ggtcccaggt 300
tcctaccaaa tgaatgggat tatcaatgaa acacataatg ggaggcatgc ttcagtctcc 360
aaggttgttg agttttgtac ggcacttggt ggcaaaacac caattcacag tgtattagtg 420
gccaacaatg gaatggcagc agctaagttc atgcggagtg tccgaacatg ggctaatgat 480
acttttggat cagagaaggc aattcagctg atagctatgg caactccgga ggatctgagg 540
ataaatgcag agcacatcag aattgccgat caatttgtag aggtacctgg tggaacaaac 600
aacaacaact atgcaaatgt ccaactcata gtggagatag cagagagaac aggtgtttct 660
gctgtttggc ctggttgggg tcatgcatct gagaatcctg aacttccaga tgcgctgact 720
gcaaaaggaa ttgtttttct tgggccacca gcatcatcaa tgcatgcatt aggagacaag 780
gttggctcag ctctcattgc tcaagcagct ggagttccaa cacttgcttg gagtggatca 840
catgtggaag ttcctctgga gtgttgcttg gactcaatac ctgatgagat gtatagaaaa 900
gcttgtgtta ctaccacaga ggaagcagtt gcaagttgtc aggtggttgg ttatcctgcc 960
atgattaagg catcttgggg tggtggtggt aaaggaataa ggaaggttca taatgatgat 1020
gaggttagga cattatttaa gcaagttcaa ggcgaagtac ctggttcccc aatatttatc 1080
atgaggctag ctgctcagag tcgacatctt gaagttcagt tgctttgtga tcaatatggc 1140
aacgtagcag cacttcacag tcgagattgc agtgtacaac ggcgacacca aaagataatc 1200
gaggaaggac cagttactgt tgctcctcgt gagactgtga aagagcttga gcaggcagca 1260
cggaggcttg ctaaagctgt gggttatgtt ggtgctgcta ctgttgaata cctttacagc 1320
atggaaactg gtgaatatta ttttctggaa cttaatccac ggctacaggt tgagcatcct 1380
gtcactgagt ggatagctga agtaaatttg cctgcggctc aagttgctgt tggaatgggt 1440
ataccccttt ggcagattcc agagatcagg cgcttctacg gaatgaacca tggaggaggc 1500
tatgaccttt ggaggaaaac agcagctcta gcgactccat ttaactttga tgaagtagat 1560
tctaaatggc caaaaggcca ctgcgtagct gttagaataa ctagcgagga tccagatgat 1620
gggtttaagc ctactggtgg aaaagtaaag gagataagtt tcaagagtaa accaaatgtt 1680
tgggcctatt tctcagtaaa gtctggtgga ggcatccatg aattcgctga ttctcagttc 1740
ggacatgttt ttgcgtatgg aactactaga tcggcagcaa taactaccat ggctcttgca 1800
ctaaaagagg ttcaaattcg tggagaaatt cattcaaacg tagactacac agttgaccta 1860
ttaaatgcct cagattttag agaaaataag attcatactg gttggctgga taccaggata 1920
gccatgcgtg ttcaagctga gaggcctcca tggtatattt cagtcgttgg aggggcttta 1980
tataaaacag taactgccaa cacggccact gtttctgatt atgttggtta tcttaccaag 2040
ggccagattc caccaaagca tatatccctt gtctatacga ctgttgcttt gaatatagat 2100
gggaaaaaat atacaatcga tactgtgagg agtggacatg gtagctacag attgcgaatg 2160
aatggatcaa cggttgacgc aaatgtacaa atattatgtg atggtgggct tttaatgcag 2220
ctggatggaa acagccatgt aatttatgct gaagaagagg ccagtggtac acgacttctt 2280
attgatggaa agacatgcat gttacagaat gaccatgacc catcaaagtt attagctgag 2340
acaccatgca aacttcttcg tttcttggtt gctgatggtg ctcatgttga tgctgatgta 2400
ccatatgcgg aagttgaggt tatgaagatg tgcatgcccc tcttatcacc cgcttctggt 2460
gtcatacatg ttgtaatgtc tgagggccaa gcaatgcagg ctggtgatct tatagctagg 2520
ctggatcttg atgacccttc tgctgttaag agagctgagc cgttcgaaga tacttttcca 2580
caaatgggtc tccctattgc tgcttctggc caagttcaca aattatgtgc tgcaagtctg 2640
aatgcttgtc gaatgatcct tgcggggtat gagcatgata ttgacaaggt tgtgccagag 2700
ttggtatact gcctagacac tccggagctt cctttcctgc agtgggagga gcttatgtct 2760
gttttagcaa ctagacttcc aagaaatctt aaaagtgagt tggagggcaa atatgaggaa 2820
tacaaagtaa aatttgactc tgggataatc aatgatttcc ctgccaatat gctacgagtg 2880
ataattgagg aaaatcttgc atgtggttct gagaaggaga aggctacaaa tgagaggctt 2940
gttgagcctc ttatgagcct actgaagtca tatgagggtg ggagagaaag tcatgctcac 3000
tttgttgtca agtccctttt tgaggagtat ctctatgttg aagaattgtt cagtgatgga 3060
attcagtctg atgtgattga gcgtctgcgc cttcaacata gtaaagacct acagaaggtc 3120
gtagacattg tgttgtccca ccagagtgtt agaaataaaa ctaagctgat actaaaactc 3180
atggagagtc tggtctatcc aaatcctgct gcctacaggg atcaattgat tcgcttttct 3240
tcccttaatc acaaagcgta ttacaagttg gcacttaaag ctagtgaact tcttgaacaa 3300
acaaaactta gtgagctccg tgcaagaata gcaaggagcc tttcagagct ggagatgttt 3360
actgaggaaa gcaagggtct ctccatgcat aagcgagaaa ttgccattaa ggagagcatg 3420
gaagatttag tcactgctcc actgccagtt gaagatgcgc tcatttcttt atttgattgt 3480
agtgatacaa ctgttcaaca gagagtgatt gagacttata tagctcgatt ataccagcct 3540
catcttgtaa aggacagtat caaaatgaaa tggatagaat cgggtgttat tgctttatgg 3600
gaatttcctg aagggcattt tgatgcaaga aatggaggag cggttcttgg tgacaaaaga 3660
tggggtgcca tggtcattgt caagtctctt gaatcacttt caatggccat tagatttgca 3720
ctaaaggaga catcacacta cactagctct gagggcaata tgatgcatat tgctttgttg 3780
ggtgctgata ataagatgca tataattcaa gaaagtggtg atgatgctga cagaatagcc 3840
aaacttccct tgatactaaa ggataatgta accgatctgc atgcctctgg tgtgaaaaca 3900
ataagtttca ttgttcaaag agatgaagca cggatgacaa tgcgtcgtac cttcctttgg 3960
tctgatgaaa agctttctta tgaggaagag ccaattctcc ggcatgtgga acctcctctt 4020
tctgcacttc ttgagttgga caagttgaaa gtgaaaggat acaatgaaat gaagtatacc 4080
ccatcacggg atcgtcaatg gcatatctac acacttagaa atactgaaaa ccccaaaatg 4140
ttgcaccggg tatttttccg aacccttgtc aggcaaccca gtgtatccaa caagttttct 4200
tcgggccaga ttggtgacat ggaagttggg agtgctgaag aacctctgtc atttacatca 4260
accagcatat taagatcttt gatgactgct atagaggaat tggagcttca cgcaattaga 4320
actggccatt cacacatgta tttgcatgta ttgaaagaac aaaagcttct tgatcttgtt 4380
ccagtttcag ggaatacagt tttggatgtt ggtcaagatg aagctactgc atattcactt 4440
ttaaaagaaa tggctatgaa gatacatgaa cttgttggtg caagaatgca ccatctttct 4500
gtatgccaat gggaagtgaa acttaagttg gactgcgatg gtcctgccag tggtacctgg 4560
aggattgtaa caaccaatgt tactagtcac acttgcactg tggatatcta ccgtgagatg 4620
gaagataaag aatcacggaa gttagtatac catcccgcca ctccggcggc tggtcctctg 4680
catggtgtgg cactgaataa tccatatcag cctttgagtg tcattgatct caaacgctgt 4740
tctgctagga ataatagaac tacatactgc tatgattttc cactggcatt tgaaactgca 4800
gtgaggaagt catggtcctc tagtacctct ggtgcttcta aaggtgttga aaatgcccaa 4860
tgttatgtta aagctacaga gttggtattt gcggacaaac atgggtcatg gggcactcct 4920
ttagttcaaa tggaccggcc tgctgggctc aatgacattg gtatggtagc ttggaccttg 4980
aagatgtcca ctcctgaatt tcctagtggt agggagatta ttgttgttgc aaatgatatt 5040
acgttcagag ctggatcatt tggcccaagg gaagatgcat tttttgaagc tgttaccaac 5100
ctagcctgtg agaagaaact tcctcttatt tatttggcag caaattctgg tgctcgaatt 5160
ggcatagcag atgaagtgaa atcttgcttc cgtgttgggt ggtctgatga tggcagccct 5220
gaacgtgggt ttcagtacat ttatctaagc gaagaagact atgctcgtat tggcacttct 5280
gtcatagcac ataagatgca gctagacagt ggtgaaatta ggtgggttat tgattctgtt 5340
gtgggcaagg aagatggact tggtgtggag aatatacatg gaagtgctgc tattgccagt 5400
gcttattcta gggcatataa ggagacattt acacttacat ttgtgactgg aagaactgtt 5460
ggaataggag cttatcttgc tcgacttggc atccggtgca tacagcgtct tgaccagcct 5520
attattctta caggctattc tgcactgaac aagcttcttg ggcgggaagt gtacagctcc 5580
cacatgcagt tgggtggtcc caaaatcatg gcaactaatg gtgttgtcca tcttactgtt 5640
tcagatgacc ttgaaggcgt ttctaatata ttgaggtggc tcagttatgt tcctgcctac 5700
attggtggac cacttccagt aacaacaccg ttggacccac cggacagacc tgttgcatac 5760
attcctgaga actcgtgtga tcctcgagcg gctatccgtg gtgttgatga cagccaaggg 5820
aaatggttag gtggtatgtt tgataaagac agctttgtgg aaacatttga aggttgggct 5880
aagacagtgg ttactggcag agcaaagctt ggtggaattc cagtgggtgt gatagctgtg 5940
gagactcaga ccatgatgca aactatccct gctgaccctg gtcagcttga ttcccgtgag 6000
caatctgttc ctcgtgctgg acaagtgtgg tttccagatt ctgcaaccaa gactgcgcag 6060
gcattgctgg acttcaaccg tgaaggatta cctctgttca tcctcgctaa ctggagaggc 6120
ttctctggtg gacaaagaga tctttttgaa ggaattcttc aggctggctc gactattgtt 6180
gagaacctta ggacatacaa tcagcctgcc tttgtctaca ttcccatggc tgcagagcta 6240
cgaggagggg cttgggttgt ggttgatagc aagataaacc cagaccgcat tgagtgctat 6300
gctgagagga ctgcaaaagg caatgttctg gaaccgcaag ggttaattga gatcaagttc 6360
aggtcagagg aactccagga ttgcatgagt cggcttgacc caacattaat tgatctgaaa 6420
gcaaaactcg aagtagcaaa taaaaatgga agtgctgaca caaaatcgct tcaagaaaat 6480
atagaagctc gaacaaaaca gttgatgcct ctatatactc agattgcgat acggtttgct 6540
gaattgcatg atacatccct cagaatggct gcgaaaggtg tgattaagaa agttgtggac 6600
tgggaagaat cacgatcttt cttctataag agattacgga ggaggatctc tgaggatgtt 6660
cttgcaaaag aaattagagc tgtagcaggt gagcagtttt cccaccaacc agcaatcgag 6720
ctgatcaaga aatggtattc agcttcacat gcagctgaat gggatgatga cgatgctttt 6780
gttgcttgga tggataaccc tgaaaactac aaggattata ttcaatatct taaggctcaa 6840
agagtatccc aatccctctc aagtctttca gattccagct cagatttgca agccctgcca 6900
cagggtcttt ccatgttact agataagatg gatccctcta gaagagctca acttgttgaa 6960
gaaatcagga aggtccttgg ttga 6984
<210> 12
<211> 2327
<212> PRT
<213> 水稻镇糯19野生型基因(Oryza sativa Japonica Group zhen19)
<400> 12
Met Thr Ser Thr His Val Ala Thr Leu Gly Val Gly Ala Gln Ala Pro
1 5 10 15
Pro Arg His Gln Lys Lys Ser Ala Gly Thr Ala Phe Val Ser Ser Gly
20 25 30
Ser Ser Arg Pro Ser Tyr Arg Lys Asn Gly Gln Arg Thr Arg Ser Leu
35 40 45
Arg Glu Glu Ser Asn Gly Gly Val Ser Asp Ser Lys Lys Leu Asn His
50 55 60
Ser Ile Arg Gln Gly Leu Ala Gly Ile Ile Asp Leu Pro Asn Asp Ala
65 70 75 80
Ala Ser Glu Val Asp Ile Ser His Gly Ser Glu Asp Pro Arg Gly Pro
85 90 95
Thr Val Pro Gly Ser Tyr Gln Met Asn Gly Ile Ile Asn Glu Thr His
100 105 110
Asn Gly Arg His Ala Ser Val Ser Lys Val Val Glu Phe Cys Thr Ala
115 120 125
Leu Gly Gly Lys Thr Pro Ile His Ser Val Leu Val Ala Asn Asn Gly
130 135 140
Met Ala Ala Ala Lys Phe Met Arg Ser Val Arg Thr Trp Ala Asn Asp
145 150 155 160
Thr Phe Gly Ser Glu Lys Ala Ile Gln Leu Ile Ala Met Ala Thr Pro
165 170 175
Glu Asp Leu Arg Ile Asn Ala Glu His Ile Arg Ile Ala Asp Gln Phe
180 185 190
Val Glu Val Pro Gly Gly Thr Asn Asn Asn Asn Tyr Ala Asn Val Gln
195 200 205
Leu Ile Val Glu Ile Ala Glu Arg Thr Gly Val Ser Ala Val Trp Pro
210 215 220
Gly Trp Gly His Ala Ser Glu Asn Pro Glu Leu Pro Asp Ala Leu Thr
225 230 235 240
Ala Lys Gly Ile Val Phe Leu Gly Pro Pro Ala Ser Ser Met His Ala
245 250 255
Leu Gly Asp Lys Val Gly Ser Ala Leu Ile Ala Gln Ala Ala Gly Val
260 265 270
Pro Thr Leu Ala Trp Ser Gly Ser His Val Glu Val Pro Leu Glu Cys
275 280 285
Cys Leu Asp Ser Ile Pro Asp Glu Met Tyr Arg Lys Ala Cys Val Thr
290 295 300
Thr Thr Glu Glu Ala Val Ala Ser Cys Gln Val Val Gly Tyr Pro Ala
305 310 315 320
Met Ile Lys Ala Ser Trp Gly Gly Gly Gly Lys Gly Ile Arg Lys Val
325 330 335
His Asn Asp Asp Glu Val Arg Thr Leu Phe Lys Gln Val Gln Gly Glu
340 345 350
Val Pro Gly Ser Pro Ile Phe Ile Met Arg Leu Ala Ala Gln Ser Arg
355 360 365
His Leu Glu Val Gln Leu Leu Cys Asp Gln Tyr Gly Asn Val Ala Ala
370 375 380
Leu His Ser Arg Asp Cys Ser Val Gln Arg Arg His Gln Lys Ile Ile
385 390 395 400
Glu Glu Gly Pro Val Thr Val Ala Pro Arg Glu Thr Val Lys Glu Leu
405 410 415
Glu Gln Ala Ala Arg Arg Leu Ala Lys Ala Val Gly Tyr Val Gly Ala
420 425 430
Ala Thr Val Glu Tyr Leu Tyr Ser Met Glu Thr Gly Glu Tyr Tyr Phe
435 440 445
Leu Glu Leu Asn Pro Arg Leu Gln Val Glu His Pro Val Thr Glu Trp
450 455 460
Ile Ala Glu Val Asn Leu Pro Ala Ala Gln Val Ala Val Gly Met Gly
465 470 475 480
Ile Pro Leu Trp Gln Ile Pro Glu Ile Arg Arg Phe Tyr Gly Met Asn
485 490 495
His Gly Gly Gly Tyr Asp Leu Trp Arg Lys Thr Ala Ala Leu Ala Thr
500 505 510
Pro Phe Asn Phe Asp Glu Val Asp Ser Lys Trp Pro Lys Gly His Cys
515 520 525
Val Ala Val Arg Ile Thr Ser Glu Asp Pro Asp Asp Gly Phe Lys Pro
530 535 540
Thr Gly Gly Lys Val Lys Glu Ile Ser Phe Lys Ser Lys Pro Asn Val
545 550 555 560
Trp Ala Tyr Phe Ser Val Lys Ser Gly Gly Gly Ile His Glu Phe Ala
565 570 575
Asp Ser Gln Phe Gly His Val Phe Ala Tyr Gly Thr Thr Arg Ser Ala
580 585 590
Ala Ile Thr Thr Met Ala Leu Ala Leu Lys Glu Val Gln Ile Arg Gly
595 600 605
Glu Ile His Ser Asn Val Asp Tyr Thr Val Asp Leu Leu Asn Ala Ser
610 615 620
Asp Phe Arg Glu Asn Lys Ile His Thr Gly Trp Leu Asp Thr Arg Ile
625 630 635 640
Ala Met Arg Val Gln Ala Glu Arg Pro Pro Trp Tyr Ile Ser Val Val
645 650 655
Gly Gly Ala Leu Tyr Lys Thr Val Thr Ala Asn Thr Ala Thr Val Ser
660 665 670
Asp Tyr Val Gly Tyr Leu Thr Lys Gly Gln Ile Pro Pro Lys His Ile
675 680 685
Ser Leu Val Tyr Thr Thr Val Ala Leu Asn Ile Asp Gly Lys Lys Tyr
690 695 700
Thr Ile Asp Thr Val Arg Ser Gly His Gly Ser Tyr Arg Leu Arg Met
705 710 715 720
Asn Gly Ser Thr Val Asp Ala Asn Val Gln Ile Leu Cys Asp Gly Gly
725 730 735
Leu Leu Met Gln Leu Asp Gly Asn Ser His Val Ile Tyr Ala Glu Glu
740 745 750
Glu Ala Ser Gly Thr Arg Leu Leu Ile Asp Gly Lys Thr Cys Met Leu
755 760 765
Gln Asn Asp His Asp Pro Ser Lys Leu Leu Ala Glu Thr Pro Cys Lys
770 775 780
Leu Leu Arg Phe Leu Val Ala Asp Gly Ala His Val Asp Ala Asp Val
785 790 795 800
Pro Tyr Ala Glu Val Glu Val Met Lys Met Cys Met Pro Leu Leu Ser
805 810 815
Pro Ala Ser Gly Val Ile His Val Val Met Ser Glu Gly Gln Ala Met
820 825 830
Gln Ala Gly Asp Leu Ile Ala Arg Leu Asp Leu Asp Asp Pro Ser Ala
835 840 845
Val Lys Arg Ala Glu Pro Phe Glu Asp Thr Phe Pro Gln Met Gly Leu
850 855 860
Pro Ile Ala Ala Ser Gly Gln Val His Lys Leu Cys Ala Ala Ser Leu
865 870 875 880
Asn Ala Cys Arg Met Ile Leu Ala Gly Tyr Glu His Asp Ile Asp Lys
885 890 895
Val Val Pro Glu Leu Val Tyr Cys Leu Asp Thr Pro Glu Leu Pro Phe
900 905 910
Leu Gln Trp Glu Glu Leu Met Ser Val Leu Ala Thr Arg Leu Pro Arg
915 920 925
Asn Leu Lys Ser Glu Leu Glu Gly Lys Tyr Glu Glu Tyr Lys Val Lys
930 935 940
Phe Asp Ser Gly Ile Ile Asn Asp Phe Pro Ala Asn Met Leu Arg Val
945 950 955 960
Ile Ile Glu Glu Asn Leu Ala Cys Gly Ser Glu Lys Glu Lys Ala Thr
965 970 975
Asn Glu Arg Leu Val Glu Pro Leu Met Ser Leu Leu Lys Ser Tyr Glu
980 985 990
Gly Gly Arg Glu Ser His Ala His Phe Val Val Lys Ser Leu Phe Glu
995 1000 1005
Glu Tyr Leu Tyr Val Glu Glu Leu Phe Ser Asp Gly Ile Gln Ser Asp
1010 1015 1020
Val Ile Glu Arg Leu Arg Leu Gln His Ser Lys Asp Leu Gln Lys Val
1025 1030 1035 1040
Val Asp Ile Val Leu Ser His Gln Ser Val Arg Asn Lys Thr Lys Leu
1045 1050 1055
Ile Leu Lys Leu Met Glu Ser Leu Val Tyr Pro Asn Pro Ala Ala Tyr
1060 1065 1070
Arg Asp Gln Leu Ile Arg Phe Ser Ser Leu Asn His Lys Ala Tyr Tyr
1075 1080 1085
Lys Leu Ala Leu Lys Ala Ser Glu Leu Leu Glu Gln Thr Lys Leu Ser
1090 1095 1100
Glu Leu Arg Ala Arg Ile Ala Arg Ser Leu Ser Glu Leu Glu Met Phe
1105 1110 1115 1120
Thr Glu Glu Ser Lys Gly Leu Ser Met His Lys Arg Glu Ile Ala Ile
1125 1130 1135
Lys Glu Ser Met Glu Asp Leu Val Thr Ala Pro Leu Pro Val Glu Asp
1140 1145 1150
Ala Leu Ile Ser Leu Phe Asp Cys Ser Asp Thr Thr Val Gln Gln Arg
1155 1160 1165
Val Ile Glu Thr Tyr Ile Ala Arg Leu Tyr Gln Pro His Leu Val Lys
1170 1175 1180
Asp Ser Ile Lys Met Lys Trp Ile Glu Ser Gly Val Ile Ala Leu Trp
1185 1190 1195 1200
Glu Phe Pro Glu Gly His Phe Asp Ala Arg Asn Gly Gly Ala Val Leu
1205 1210 1215
Gly Asp Lys Arg Trp Gly Ala Met Val Ile Val Lys Ser Leu Glu Ser
1220 1225 1230
Leu Ser Met Ala Ile Arg Phe Ala Leu Lys Glu Thr Ser His Tyr Thr
1235 1240 1245
Ser Ser Glu Gly Asn Met Met His Ile Ala Leu Leu Gly Ala Asp Asn
1250 1255 1260
Lys Met His Ile Ile Gln Glu Ser Gly Asp Asp Ala Asp Arg Ile Ala
1265 1270 1275 1280
Lys Leu Pro Leu Ile Leu Lys Asp Asn Val Thr Asp Leu His Ala Ser
1285 1290 1295
Gly Val Lys Thr Ile Ser Phe Ile Val Gln Arg Asp Glu Ala Arg Met
1300 1305 1310
Thr Met Arg Arg Thr Phe Leu Trp Ser Asp Glu Lys Leu Ser Tyr Glu
1315 1320 1325
Glu Glu Pro Ile Leu Arg His Val Glu Pro Pro Leu Ser Ala Leu Leu
1330 1335 1340
Glu Leu Asp Lys Leu Lys Val Lys Gly Tyr Asn Glu Met Lys Tyr Thr
1345 1350 1355 1360
Pro Ser Arg Asp Arg Gln Trp His Ile Tyr Thr Leu Arg Asn Thr Glu
1365 1370 1375
Asn Pro Lys Met Leu His Arg Val Phe Phe Arg Thr Leu Val Arg Gln
1380 1385 1390
Pro Ser Val Ser Asn Lys Phe Ser Ser Gly Gln Ile Gly Asp Met Glu
1395 1400 1405
Val Gly Ser Ala Glu Glu Pro Leu Ser Phe Thr Ser Thr Ser Ile Leu
1410 1415 1420
Arg Ser Leu Met Thr Ala Ile Glu Glu Leu Glu Leu His Ala Ile Arg
1425 1430 1435 1440
Thr Gly His Ser His Met Tyr Leu His Val Leu Lys Glu Gln Lys Leu
1445 1450 1455
Leu Asp Leu Val Pro Val Ser Gly Asn Thr Val Leu Asp Val Gly Gln
1460 1465 1470
Asp Glu Ala Thr Ala Tyr Ser Leu Leu Lys Glu Met Ala Met Lys Ile
1475 1480 1485
His Glu Leu Val Gly Ala Arg Met His His Leu Ser Val Cys Gln Trp
1490 1495 1500
Glu Val Lys Leu Lys Leu Asp Cys Asp Gly Pro Ala Ser Gly Thr Trp
1505 1510 1515 1520
Arg Ile Val Thr Thr Asn Val Thr Ser His Thr Cys Thr Val Asp Ile
1525 1530 1535
Tyr Arg Glu Met Glu Asp Lys Glu Ser Arg Lys Leu Val Tyr His Pro
1540 1545 1550
Ala Thr Pro Ala Ala Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro
1555 1560 1565
Tyr Gln Pro Leu Ser Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn
1570 1575 1580
Asn Arg Thr Thr Tyr Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala
1585 1590 1595 1600
Val Arg Lys Ser Trp Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val
1605 1610 1615
Glu Asn Ala Gln Cys Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp
1620 1625 1630
Lys His Gly Ser Trp Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala
1635 1640 1645
Gly Leu Asn Asp Ile Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr
1650 1655 1660
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1665 1670 1675 1680
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
1685 1690 1695
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
1700 1705 1710
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
1715 1720 1725
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
1730 1735 1740
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
1745 1750 1755 1760
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
1765 1770 1775
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile
1780 1785 1790
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
1795 1800 1805
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
1810 1815 1820
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
1825 1830 1835 1840
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
1845 1850 1855
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
1860 1865 1870
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
1875 1880 1885
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
1890 1895 1900
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
1905 1910 1915 1920
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
1925 1930 1935
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
1940 1945 1950
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
1955 1960 1965
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
1970 1975 1980
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
1985 1990 1995 2000
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
2005 2010 2015
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
2020 2025 2030
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
2035 2040 2045
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
2050 2055 2060
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
2065 2070 2075 2080
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
2085 2090 2095
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
2100 2105 2110
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
2115 2120 2125
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
2130 2135 2140
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
2145 2150 2155 2160
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
2165 2170 2175
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
2180 2185 2190
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
2195 2200 2205
Tyr Lys Arg Leu Arg Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu
2210 2215 2220
Ile Arg Ala Val Ala Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu
2225 2230 2235 2240
Leu Ile Lys Lys Trp Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp
2245 2250 2255
Asp Asp Ala Phe Val Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp
2260 2265 2270
Tyr Ile Gln Tyr Leu Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser
2275 2280 2285
Leu Ser Asp Ser Ser Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser
2290 2295 2300
Met Leu Leu Asp Lys Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu
2305 2310 2315 2320
Glu Ile Arg Lys Val Leu Gly
2325
<210> 13
<211> 24
<212> DNA
<213> ACCase正向引物(Artificial Sequence)
<400> 13
gtcagatttc acacatctgg ggat 24
<210> 14
<211> 24
<212> DNA
<213> ACCase反向引物(Artificial Sequence)
<400> 14
acttgcactt tcatctggca gaac 24

Claims (12)

1.一种水稻ACCase突变型蛋白，所述ACCase突变型蛋白的氨基酸序列存在以下任意一种或几种突变：其对应于水稻ACCase的氨基酸序列的第1792位、第2015位、第2038位、第2052位、第2089位发生突变。
2.一种水稻ACCase突变型蛋白，包括：
（a）其氨基酸序列如SEQ ID NO. 2或SEQ ID NO. 4或SEQ ID NO. 6或SEQ ID NO. 8或SEQ ID NO. 10所示；或
（b）在（a）中的氨基酸序列经过取代和/或缺失和/或添加一个或几个氨基酸且具有乙酰辅酶A羧化酶活性的由（a）衍生的蛋白质。
3.核酸或基因，其编码权利要求1~2任一项所述的蛋白。
4.根据权利要求3所述的核酸或基因，包括：
（a）其编码权利要求1~2任一项所述的蛋白；或
（b）在严格条件下与（a）限定的核苷酸序列杂交且编码具有乙酰辅酶A羧化酶活性的蛋白质的核苷酸序列；或
（c）其核苷酸序列如SEQ ID NO:1或SEQ ID NO:3或SEQ ID NO:5或SEQ ID NO:7或SEQID NO:9所示。
5.表达盒、重组载体或细胞，其含有权利要求3或4所述的核酸或基因。
6.权利要求1或2所述的水稻ACCase突变型蛋白、权利要求3或4所述的核酸或基因，权利要求5所述的表达盒、重组载体或细胞在植物抗除草剂方面的应用。
7.根据权利要求6所述的应用，其特征在于，所述除草剂是喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或几种。
8.获得具有除草剂抗性植物的方法，其特征在于，包括如下步骤：
1）使植物包含权利要求3或4所述的核酸或基因；或
2）使植物表达权利要求1或2所述的水稻ACCase突变型蛋白。
9.根据权利要求8所述的方法，其特征在于，其包括crispr基因编辑、转基因、杂交、回交或无性繁殖步骤。
10.鉴定植物的方法，其中植物是包含权利要求3或4所述的核酸或基因的植物、表达权利要求1~2之任一所述的蛋白的植物或权利要求8~9之任一所述的方法获得的植物，其特征在于，包括以下步骤：
1) 测定所述植物是否包含权利要3或4所述的核酸或基因；或，
2) 测定所述植物是否表达权利要求1~2之任一所述的蛋白。
11.一种控制杂草的方法，其特征在于，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含权利要求3或4所述的核酸或基因或权利要求5所述的表达盒、重组载体或细胞，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或几种。
12.一种用于保护植物免受由除草剂引起的损伤的方法，其特征在于，包括：对种植作物的大田施用有效剂量的除草剂，所述作物包含权利要求3或4所述的核酸或基因或权利要求5所述的表达盒、重组载体导入植物，导入后的植物产生除草剂抗性蛋白，所述除草剂为喹禾灵、吡氟氯禾灵、烯草酮、吡氟禾草灵中的一种或几种。
CN201810030101.1A 2018-01-12 2018-01-12 使植物具有除草剂抗性的ACCase突变型蛋白及其应用 Pending CN108359646A (zh)

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PCT/CN2018/093006 WO2019136938A1 (zh) 2018-01-12 2018-06-27 使植物具有除草剂抗性的ACCase突变型蛋白及其应用

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109082416A (zh) * 2018-08-22 2018-12-25 江苏省农业科学院 具有除草剂抗性的ACCase突变型基因和蛋白及其应用
CN109371000A (zh) * 2018-10-10 2019-02-22 江苏省农业科学院 一种水稻ACCase突变型基因及其在植物抗除草剂中的应用
CN112410308A (zh) * 2020-11-20 2021-02-26 江苏省农业科学院 水稻ACCase突变型基因及其蛋白在植物抗除草剂中的应用
CN112813064A (zh) * 2019-11-18 2021-05-18 安徽省农业科学院水稻研究所 一种创制高抗稳定的内源抗除草剂水稻的方法
CN112824526A (zh) * 2019-11-18 2021-05-21 安徽省农业科学院水稻研究所 一种水稻ACCase突变型蛋白及相应基因
CN113005103A (zh) * 2019-12-21 2021-06-22 科稷达隆生物技术有限公司 一组抗除草剂蛋白及其在植物育种中的应用
CN113201557A (zh) * 2021-05-10 2021-08-03 安徽省农业科学院水稻研究所 一种引导编辑***介导作物产生内源除草剂抗性的方法
CN114032218A (zh) * 2021-02-08 2022-02-11 山东舜丰生物科技有限公司 新型抗除草剂乙酰辅酶a羧化酶突变体及其应用
WO2022063136A1 (zh) * 2020-09-23 2022-03-31 山东舜丰生物科技有限公司 抗除草剂乙酰辅酶a羧化酶突变体及其应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103796507A (zh) * 2011-02-01 2014-05-14 科罗拉多小麦研究基金会公司 乙酰辅酶a羧化酶除草剂抗性植物
CN106455514A (zh) * 2014-03-14 2017-02-22 希博斯美国有限公司 采用寡核苷酸介导的基因修复提高靶向基因修饰的效率的方法和组合物

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20100048405A1 (en) * 2008-06-20 2010-02-25 University Of Georgia Research Foundation Development of herbicide-resistant grass species
TW201113376A (en) * 2009-09-01 2011-04-16 Basf Agrochemical Products Bv Herbicide-tolerant plants
BR112014001434A2 (pt) * 2011-07-22 2018-07-31 Ricetec Ag métodos e composições para produzir arroz resistente a inibidores da accase
CN103882121A (zh) * 2014-02-27 2014-06-25 南京农业大学 基于dCAPS技术对日本看麦娘抗药性相关突变的分子检测方法

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103796507A (zh) * 2011-02-01 2014-05-14 科罗拉多小麦研究基金会公司 乙酰辅酶a羧化酶除草剂抗性植物
CN106455514A (zh) * 2014-03-14 2017-02-22 希博斯美国有限公司 采用寡核苷酸介导的基因修复提高靶向基因修饰的效率的方法和组合物

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
朱晓磊等: "乙酰辅酶A羧化酶除草剂的抗性研究进展", 《华中师范大学学报（自然科学版）》 *

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109082416B (zh) * 2018-08-22 2021-10-26 江苏省农业科学院 具有除草剂抗性的ACCase突变型基因和蛋白及其应用
CN109082416A (zh) * 2018-08-22 2018-12-25 江苏省农业科学院 具有除草剂抗性的ACCase突变型基因和蛋白及其应用
CN109371000A (zh) * 2018-10-10 2019-02-22 江苏省农业科学院 一种水稻ACCase突变型基因及其在植物抗除草剂中的应用
CN109371000B (zh) * 2018-10-10 2021-10-26 江苏省农业科学院 一种水稻ACCase突变型基因及其在植物抗除草剂中的应用
CN112813064A (zh) * 2019-11-18 2021-05-18 安徽省农业科学院水稻研究所 一种创制高抗稳定的内源抗除草剂水稻的方法
CN112824526A (zh) * 2019-11-18 2021-05-21 安徽省农业科学院水稻研究所 一种水稻ACCase突变型蛋白及相应基因
CN113005103A (zh) * 2019-12-21 2021-06-22 科稷达隆生物技术有限公司 一组抗除草剂蛋白及其在植物育种中的应用
WO2022063136A1 (zh) * 2020-09-23 2022-03-31 山东舜丰生物科技有限公司 抗除草剂乙酰辅酶a羧化酶突变体及其应用
CN112410308A (zh) * 2020-11-20 2021-02-26 江苏省农业科学院 水稻ACCase突变型基因及其蛋白在植物抗除草剂中的应用
CN112410308B (zh) * 2020-11-20 2023-11-10 江苏省农业科学院 水稻ACCase突变型基因及其蛋白在植物抗除草剂中的应用
CN114032218A (zh) * 2021-02-08 2022-02-11 山东舜丰生物科技有限公司 新型抗除草剂乙酰辅酶a羧化酶突变体及其应用
WO2022166955A1 (zh) * 2021-02-08 2022-08-11 山东舜丰生物科技有限公司 新型抗除草剂乙酰辅酶a羧化酶突变体及其应用
CN113201557A (zh) * 2021-05-10 2021-08-03 安徽省农业科学院水稻研究所 一种引导编辑***介导作物产生内源除草剂抗性的方法

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