CL2013003596A1 - Metodo para la purificacion de anticuerpos a partir de una mezcla de proteínas producidas en un biorreactor que comprende una cromatografia de intercambio iónico; unidad operativa que puede usarse con el metodo antes indicado. - Google Patents

Metodo para la purificacion de anticuerpos a partir de una mezcla de proteínas producidas en un biorreactor que comprende una cromatografia de intercambio iónico; unidad operativa que puede usarse con el metodo antes indicado.

Info

Publication number
CL2013003596A1
CL2013003596A1 CL2013003596A CL2013003596A CL2013003596A1 CL 2013003596 A1 CL2013003596 A1 CL 2013003596A1 CL 2013003596 A CL2013003596 A CL 2013003596A CL 2013003596 A CL2013003596 A CL 2013003596A CL 2013003596 A1 CL2013003596 A1 CL 2013003596A1
Authority
CL
Chile
Prior art keywords
bioreactor
antibodies
purification
mixture
ion exchange
Prior art date
Application number
CL2013003596A
Other languages
English (en)
Inventor
Henderik E Veenstra
Diderik R Kremer
Islas Maria Perlasca
Mark K Doeven
Original Assignee
Dpx Holdings Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dpx Holdings Bv filed Critical Dpx Holdings Bv
Publication of CL2013003596A1 publication Critical patent/CL2013003596A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
CL2013003596A 2011-06-16 2013-12-16 Metodo para la purificacion de anticuerpos a partir de una mezcla de proteínas producidas en un biorreactor que comprende una cromatografia de intercambio iónico; unidad operativa que puede usarse con el metodo antes indicado. CL2013003596A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP11170239 2011-06-16

Publications (1)

Publication Number Publication Date
CL2013003596A1 true CL2013003596A1 (es) 2014-06-06

Family

ID=45406929

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2013003596A CL2013003596A1 (es) 2011-06-16 2013-12-16 Metodo para la purificacion de anticuerpos a partir de una mezcla de proteínas producidas en un biorreactor que comprende una cromatografia de intercambio iónico; unidad operativa que puede usarse con el metodo antes indicado.

Country Status (14)

Country Link
US (1) US20140187749A1 (es)
EP (1) EP2721052A1 (es)
JP (1) JP2014529330A (es)
KR (1) KR20140033126A (es)
CN (1) CN103619868A (es)
AR (1) AR086938A1 (es)
AU (1) AU2012269240B2 (es)
BR (1) BR112013031906A2 (es)
CA (1) CA2838476A1 (es)
CL (1) CL2013003596A1 (es)
EA (1) EA201400024A1 (es)
IL (1) IL229763A0 (es)
MX (1) MX2013014615A (es)
WO (1) WO2012171853A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9994609B2 (en) 2013-03-15 2018-06-12 Biogen Ma Inc. Hydrophobic interaction protein chromatography under no-salt conditions
KR102140531B1 (ko) * 2018-08-07 2020-08-04 (주)휴온스 Gly-Tβ4의 제조방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2272870T3 (da) * 1998-06-09 2013-08-05 Csl Behring Ag Fremgangsmåde til fremstilling af immunoglobuliner med henblik på intravenøs indgivelse og andre immunoglobulin-lignende produkter.
WO2005044856A2 (en) 2003-10-27 2005-05-19 Wyeth Removal of high molecular weight aggregates using hydroxyapatite chromatography
DE10355251A1 (de) * 2003-11-26 2005-06-23 Merck Patent Gmbh Pharmazeutische Zubereitung enthaltend einen Antikörper gegen den EGF-Rezeptor
NZ548126A (en) 2004-02-27 2009-10-30 Ge Healthcare Bio Sciences Ab A process for the purification of antibodies involving addition of a second resin
US20110257378A1 (en) 2008-11-25 2011-10-20 Ge Healthcare Bio-Sciences Ab Aqueous two phase extraction augmented precipitation process for purification of therapeutic proteins
JP5791602B2 (ja) * 2009-08-07 2015-10-07 イー・エム・デイー・ミリポア・コーポレイシヨン 試料中の１つ以上の不純物から標的タンパク質を精製するための方法
US9527010B2 (en) * 2009-09-25 2016-12-27 Ge Healthcare Bio-Sciences Corp. Separation system and method

Also Published As

Publication number Publication date
US20140187749A1 (en) 2014-07-03
AR086938A1 (es) 2014-02-05
JP2014529330A (ja) 2014-11-06
IL229763A0 (en) 2014-01-30
CN103619868A (zh) 2014-03-05
EP2721052A1 (en) 2014-04-23
AU2012269240A1 (en) 2013-05-02
KR20140033126A (ko) 2014-03-17
EA201400024A1 (ru) 2014-04-30
AU2012269240B2 (en) 2016-01-21
BR112013031906A2 (pt) 2016-12-13
MX2013014615A (es) 2014-02-17
CA2838476A1 (en) 2012-12-20
WO2012171853A1 (en) 2012-12-20

Similar Documents

Publication Publication Date Title
CL2014000183A1 (es) Formulación farmacéutica que contienen anticuerpos anti-pcsk9; metodo para preparar dicha formulación.
CL2013003031A1 (es) Metodo para estratificacion a base de bcma en pacientes con mieloma multiple; anticuerpo anti-bcma.
CL2012002125A1 (es) Metodo para la purificacion de anticuerpos de una mezcla de proteina en un biorreactor; unidad operacional que se utiliza en el metodo antes descrito.
CL2013003792A1 (es) Metodo para detener el crecimiento celular en un cultivo celular de mamíferos que expresan una proteina recombinante.
DK3370055T3 (da) Frem- og tilbagegående mikrotomdrivsystem
BR112014011900A2 (pt) um método de purificação de anticorpo
CL2014001123A1 (es) Anticuerpos anti-il 36r; composicion farmaceutica que lo contiene; polinucleotido aislado que codifica el anticuerpo; metodo para la produccion del anticuerpo; kit de diagnostico que lo contiene.
CR20140169A (es) Proteinas de union al antigeno cd27l
CL2014000888A1 (es) Anticuerpos anti-htra1; acido nucleico que codifica dicho anticuerpo; célula anfitriona; metodo para producir dicho anticuerpo; uso para tratar degeneración macular.
CO6801647A2 (es) Formulación para anticuerpo anti-α4β7
CL2014001073A1 (es) Anticuerpos humanizados que reconocen alfa-sinucleina ; metodo para detectar cuerpos de lewy; metodo para inhibir la agregacion de sinucleina o reducir los cuerpos de lewy.
CO6801648A2 (es) Formulación para anticuerpo anti-α4β7
IL232558B (en) A method for antibody purification
BR112013024520A2 (pt) método para preparar uma composição adsorvente,em, dispositivo de purificação de àgua
CL2013001883A1 (es) Metodo de inmunoensayo para vitamina d; inmunoensayo para detectar vitamina 25-oh d en sangre.
EP2729482A4 (en) PROCESS FOR PURIFYING FC FUSION PROTEIN
BR112013025749A2 (pt) sistemas e métodos para fracionamento de vinhaça.
BR112014013034A2 (pt) método para produzir um anticorpo
BR112013021464A2 (pt) método para reduzir a demanda química de oxigênio recalcitrante de um líquido em um sistema de água.
BR112014029107A2 (pt) método para determinar a concentração de uma espécie de polissorbato em uma mistura.
SMT201400145B (it) Metodo per la rivelazione di anticorpi aBeta-specifici in un campione biologico
EP2727930A4 (en) PROCESS FOR CLEANING PROTEINS
BR112014012519A2 (pt) método para purificar 1,3-batadieno
BR112013021775A2 (pt) bactéria corineforme, e, método para produzir um l-aminoácido
BR112013007237A2 (pt) ''método para produzir um anticorpo anti-ig-1r''