CH617963A5 - - Google Patents

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Info

Publication number: CH617963A5
Authority: CH; Switzerland
Prior art keywords: process according; proteolytic enzyme; inhibitor; protease inhibitor; filtrate
Prior art date: 1973-10-05

Application number

CH1426573A

Other languages

English (en)

Italian (it)

Inventor

Gianfranco Fedeli

Luigi De Ambrosi

Original Assignee

Derivati Organici Lab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1973-10-05

Filing date

1973-10-05

Publication date

1980-06-30

1973-10-05 Application filed by Derivati Organici Lab filed Critical Derivati Organici Lab

1973-10-05 Priority to CH1426573A priority Critical patent/CH617963A5/it

1974-04-25 Priority to JP49046059A priority patent/JPS619286B2/ja

1974-10-01 Priority to DD181437A priority patent/DD118654A1/xx

1974-10-02 Priority to DE2447050A priority patent/DE2447050C2/de

1974-10-04 Priority to SU742068151A priority patent/SU611595A3/ru

1974-10-04 Priority to AR255931A priority patent/AR200699A1/es

1974-10-04 Priority to ES430685A priority patent/ES430685A1/es

1974-10-04 Priority to CA210,749A priority patent/CA1034495A/fr

1974-10-04 Priority to NL7413127A priority patent/NL7413127A/xx

1974-10-04 Priority to DK522174AA priority patent/DK139913B/da

1974-10-04 Priority to FR7433572A priority patent/FR2246567B1/fr

1978-06-19 Priority to US05/916,536 priority patent/US4216293A/en

1980-06-30 Publication of CH617963A5 publication Critical patent/CH617963A5/it

Links

238000000034 method Methods 0.000 claims description 27
210000000056 organ Anatomy 0.000 claims description 18
229940124158 Protease/peptidase inhibitor Drugs 0.000 claims description 16
239000003112 inhibitor Substances 0.000 claims description 16
239000000137 peptide hydrolase inhibitor Substances 0.000 claims description 16
102000035195 Peptidases Human genes 0.000 claims description 14
108091005804 Peptidases Proteins 0.000 claims description 14
239000004365 Protease Substances 0.000 claims description 14
HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 claims description 13
238000000605 extraction Methods 0.000 claims description 13
229920000669 heparin Polymers 0.000 claims description 13
229960002897 heparin Drugs 0.000 claims description 13
210000004072 lung Anatomy 0.000 claims description 13
210000001557 animal structure Anatomy 0.000 claims description 9
239000000706 filtrate Substances 0.000 claims description 9
241000283690 Bos taurus Species 0.000 claims description 7
108090000526 Papain Proteins 0.000 claims description 7
235000019834 papain Nutrition 0.000 claims description 7
229940055729 papain Drugs 0.000 claims description 7
239000000243 solution Substances 0.000 claims description 6
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims description 6
108010004032 Bromelains Proteins 0.000 claims description 5
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 5
229910052921 ammonium sulfate Inorganic materials 0.000 claims description 5
239000001166 ammonium sulphate Substances 0.000 claims description 5
235000011130 ammonium sulphate Nutrition 0.000 claims description 5
235000019835 bromelain Nutrition 0.000 claims description 5
238000001914 filtration Methods 0.000 claims description 5
125000001453 quaternary ammonium group Chemical group 0.000 claims description 5
239000008135 aqueous vehicle Substances 0.000 claims description 4
238000010438 heat treatment Methods 0.000 claims description 4
239000006166 lysate Substances 0.000 claims description 4
238000001556 precipitation Methods 0.000 claims description 4
108060005987 Kallikrein Proteins 0.000 claims description 3
102000001399 Kallikrein Human genes 0.000 claims description 3
108090000631 Trypsin Proteins 0.000 claims description 3
102000004142 Trypsin Human genes 0.000 claims description 3
NEUSVAOJNUQRTM-UHFFFAOYSA-N cetylpyridinium Chemical group CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 NEUSVAOJNUQRTM-UHFFFAOYSA-N 0.000 claims description 3
229960004830 cetylpyridinium Drugs 0.000 claims description 3
239000012588 trypsin Substances 0.000 claims description 3
239000007864 aqueous solution Substances 0.000 claims description 2
238000000227 grinding Methods 0.000 claims description 2
230000037452 priming Effects 0.000 claims 1
AISMNBXOJRHCIA-UHFFFAOYSA-N trimethylazanium;bromide Chemical compound Br.CN(C)C AISMNBXOJRHCIA-UHFFFAOYSA-N 0.000 claims 1
230000000694 effects Effects 0.000 description 9
238000000746 purification Methods 0.000 description 9
239000007788 liquid Substances 0.000 description 8
239000002244 precipitate Substances 0.000 description 7
239000012264 purified product Substances 0.000 description 7
238000004519 manufacturing process Methods 0.000 description 6
239000012043 crude product Substances 0.000 description 4
230000009089 cytolysis Effects 0.000 description 4
238000005194 fractionation Methods 0.000 description 4
239000000843 powder Substances 0.000 description 4
229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 4
239000000126 substance Substances 0.000 description 4
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
239000001110 calcium chloride Substances 0.000 description 3
229910001628 calcium chloride Inorganic materials 0.000 description 3
239000003599 detergent Substances 0.000 description 3
239000012153 distilled water Substances 0.000 description 3
239000000047 product Substances 0.000 description 3
102000004169 proteins and genes Human genes 0.000 description 3
108090000623 proteins and genes Proteins 0.000 description 3
238000000926 separation method Methods 0.000 description 3
239000000725 suspension Substances 0.000 description 3
210000001519 tissue Anatomy 0.000 description 3
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
102000004190 Enzymes Human genes 0.000 description 2
108090000790 Enzymes Proteins 0.000 description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
108090000270 Ficain Proteins 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
108090000284 Pepsin A Proteins 0.000 description 2
102000057297 Pepsin A Human genes 0.000 description 2
GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 2
239000000872 buffer Substances 0.000 description 2
125000002091 cationic group Chemical group 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
239000000356 contaminant Substances 0.000 description 2
229940088598 enzyme Drugs 0.000 description 2
239000000284 extract Substances 0.000 description 2
235000019836 ficin Nutrition 0.000 description 2
POTUGHMKJGOKRI-UHFFFAOYSA-N ficin Chemical compound FI=CI=N POTUGHMKJGOKRI-UHFFFAOYSA-N 0.000 description 2
239000000463 material Substances 0.000 description 2
239000000203 mixture Substances 0.000 description 2
210000000496 pancreas Anatomy 0.000 description 2
229940111202 pepsin Drugs 0.000 description 2
238000003756 stirring Methods 0.000 description 2
229960001322 trypsin Drugs 0.000 description 2
238000005406 washing Methods 0.000 description 2
206010002198 Anaphylactic reaction Diseases 0.000 description 1
CPELXLSAUQHCOX-UHFFFAOYSA-M Bromide Chemical compound [Br-] CPELXLSAUQHCOX-UHFFFAOYSA-M 0.000 description 1
102000003670 Carboxypeptidase B Human genes 0.000 description 1
108090000087 Carboxypeptidase B Proteins 0.000 description 1
102000000496 Carboxypeptidases A Human genes 0.000 description 1
108010080937 Carboxypeptidases A Proteins 0.000 description 1
LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
108090000317 Chymotrypsin Proteins 0.000 description 1
102000029816 Collagenase Human genes 0.000 description 1
108060005980 Collagenase Proteins 0.000 description 1
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
229920002683 Glycosaminoglycan Polymers 0.000 description 1
108010067372 Pancreatic elastase Proteins 0.000 description 1
102000016387 Pancreatic elastase Human genes 0.000 description 1
108010019160 Pancreatin Proteins 0.000 description 1
206010033645 Pancreatitis Diseases 0.000 description 1
230000002378 acidificating effect Effects 0.000 description 1
230000001476 alcoholic effect Effects 0.000 description 1
229910021529 ammonia Inorganic materials 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
230000002052 anaphylactic effect Effects 0.000 description 1
230000036783 anaphylactic response Effects 0.000 description 1
208000003455 anaphylaxis Diseases 0.000 description 1
150000001450 anions Chemical class 0.000 description 1
239000008365 aqueous carrier Substances 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000008280 blood Substances 0.000 description 1
235000010633 broth Nutrition 0.000 description 1
238000001311 chemical methods and process Methods 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
229960002376 chymotrypsin Drugs 0.000 description 1
239000007979 citrate buffer Substances 0.000 description 1
229960002424 collagenase Drugs 0.000 description 1
238000007796 conventional method Methods 0.000 description 1
238000001816 cooling Methods 0.000 description 1
239000012154 double-distilled water Substances 0.000 description 1
230000008030 elimination Effects 0.000 description 1
238000003379 elimination reaction Methods 0.000 description 1
235000019441 ethanol Nutrition 0.000 description 1
239000004744 fabric Substances 0.000 description 1
239000012467 final product Substances 0.000 description 1
238000005189 flocculation Methods 0.000 description 1
230000016615 flocculation Effects 0.000 description 1
238000001641 gel filtration chromatography Methods 0.000 description 1
238000005227 gel permeation chromatography Methods 0.000 description 1
210000004907 gland Anatomy 0.000 description 1
239000002045 hydroalcoholic fraction Substances 0.000 description 1
238000009776 industrial production Methods 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
238000002955 isolation Methods 0.000 description 1
210000004185 liver Anatomy 0.000 description 1
238000005461 lubrication Methods 0.000 description 1
210000001165 lymph node Anatomy 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
230000020477 pH reduction Effects 0.000 description 1
229940055695 pancreatin Drugs 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
-1 plasmine Proteins 0.000 description 1
229920000447 polyanionic polymer Polymers 0.000 description 1
108090000765 processed proteins & peptides Proteins 0.000 description 1
239000002510 pyrogen Substances 0.000 description 1
230000001698 pyrogenic effect Effects 0.000 description 1
239000002994 raw material Substances 0.000 description 1
238000009938 salting Methods 0.000 description 1
238000005063 solubilization Methods 0.000 description 1
230000007928 solubilization Effects 0.000 description 1
230000003381 solubilizing effect Effects 0.000 description 1
210000000952 spleen Anatomy 0.000 description 1
230000001225 therapeutic effect Effects 0.000 description 1
239000002699 waste material Substances 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
- C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
- C08B37/0066—Isolation or extraction of proteoglycans from organs
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8114—Kunitz type inhibitors
- C07K14/8117—Bovine/basic pancreatic trypsin inhibitor (BPTI, aprotinin)

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Medicinal Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Molecular Biology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Polymers & Plastics (AREA)
Biophysics (AREA)
Gastroenterology & Hepatology (AREA)
Engineering & Computer Science (AREA)
Materials Engineering (AREA)
Chemical Kinetics & Catalysis (AREA)
Genetics & Genomics (AREA)
Peptides Or Proteins (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Enzymes And Modification Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Polysaccharides And Polysaccharide Derivatives (AREA)
Compounds Of Unknown Constitution (AREA)

CH1426573A 1973-10-05 1973-10-05 CH617963A5 (fr)

Priority Applications (12)

Application Number	Priority Date	Filing Date	Title
CH1426573A CH617963A5 (fr)	1973-10-05	1973-10-05
JP49046059A JPS619286B2 (fr)	1973-10-05	1974-04-25
DD181437A DD118654A1 (fr)	1973-10-05	1974-10-01
DE2447050A DE2447050C2 (de)	1973-10-05	1974-10-02	Verfahren zur Gewinnung von Proteasenhemmstoff
SU742068151A SU611595A3 (ru)	1973-10-05	1974-10-04	Способ одновременного получени ингибитора протеазы и гепарина
AR255931A AR200699A1 (es)	1973-10-05	1974-10-04	Metodo para la produccion de inhibidor de proteasa
ES430685A ES430685A1 (es)	1973-10-05	1974-10-04	Metodo para la produccion de inhibidor de proteasa, hepari-na y otros mucopolisacaridos.
CA210,749A CA1034495A (fr)	1973-10-05	1974-10-04	Methode pour la preparation d'un inhibiteur de protease
NL7413127A NL7413127A (nl)	1973-10-05	1974-10-04	Werkwijze voor het bereiden van een remmer roteasen.
DK522174AA DK139913B (da)	1973-10-05	1974-10-04	Fremgangsmåde til udvinding af kallikrein-trypsin-inhibitor og eventuelt heparin.
FR7433572A FR2246567B1 (fr)	1973-10-05	1974-10-04
US05/916,536 US4216293A (en)	1973-10-05	1978-06-19	Extracting protease-inhibitor from animal tissue containing same

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CH1426573A CH617963A5 (fr)	1973-10-05	1973-10-05

Publications (1)

Publication Number	Publication Date
CH617963A5 true CH617963A5 (fr)	1980-06-30

Family

ID=4399389

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CH1426573A CH617963A5 (fr)	1973-10-05	1973-10-05

Country Status (12)

Country	Link
US (1)	US4216293A (fr)
JP (1)	JPS619286B2 (fr)
AR (1)	AR200699A1 (fr)
CA (1)	CA1034495A (fr)
CH (1)	CH617963A5 (fr)
DD (1)	DD118654A1 (fr)
DE (1)	DE2447050C2 (fr)
DK (1)	DK139913B (fr)
ES (1)	ES430685A1 (fr)
FR (1)	FR2246567B1 (fr)
NL (1)	NL7413127A (fr)
SU (1)	SU611595A3 (fr)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE2509482C2 (de) *	1975-03-05	1985-11-21	Bayer Ag, 5090 Leverkusen	Verfahren zur Herstellung des Kallikrein-Trypsin-Inhibitors aus Rinderlunge
JPS5334911A (en) *	1976-09-01	1978-03-31	Mochida Pharm Co Ltd	Production of proteolytic enzyme inhibitor
IT1190313B (it) *	1986-04-17	1988-02-16	Crinos Industria Farmaco	Procedimento per l'ottenimento di polidesossiribonucleotidi chimicamente definiti e riproducibili e prodotto farmacologicamente attivo risultante
ATE315588T1 (de) *	1997-07-16	2006-02-15	Akzo Nobel Nv	Verfahren zur herstellung von heparin
EP1877447B1 (fr)	2005-05-05	2016-12-21	Sensient Flavors Inc.	Production de bêta-glucanes et de mannanes

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3451996A (en) *	1968-02-12	1969-06-24	Thompson Farms Co	Method for the preparation of heparin
SU377159A1 (ru)	1970-12-31	1973-04-17		Способ получения ингибитора протеаз

1973
- 1973-10-05 CH CH1426573A patent/CH617963A5/it not_active IP Right Cessation
1974
- 1974-04-25 JP JP49046059A patent/JPS619286B2/ja not_active Expired
- 1974-10-01 DD DD181437A patent/DD118654A1/xx unknown
- 1974-10-02 DE DE2447050A patent/DE2447050C2/de not_active Expired
- 1974-10-04 CA CA210,749A patent/CA1034495A/fr not_active Expired
- 1974-10-04 SU SU742068151A patent/SU611595A3/ru active
- 1974-10-04 ES ES430685A patent/ES430685A1/es not_active Expired
- 1974-10-04 DK DK522174AA patent/DK139913B/da not_active IP Right Cessation
- 1974-10-04 AR AR255931A patent/AR200699A1/es active
- 1974-10-04 NL NL7413127A patent/NL7413127A/xx not_active Application Discontinuation
- 1974-10-04 FR FR7433572A patent/FR2246567B1/fr not_active Expired
1978
- 1978-06-19 US US05/916,536 patent/US4216293A/en not_active Expired - Lifetime

Also Published As

Publication number	Publication date
AR200699A1 (es)	1974-11-29
DK139913B (da)	1979-05-14
DK139913C (fr)	1979-10-15
ES430685A1 (es)	1976-11-01
DK522174A (fr)	1975-06-09
DE2447050A1 (de)	1975-04-10
DD118654A1 (fr)	1976-03-12
FR2246567A1 (fr)	1975-05-02
DE2447050C2 (de)	1983-07-28
US4216293A (en)	1980-08-05
JPS619286B2 (fr)	1986-03-22
FR2246567B1 (fr)	1978-03-24
JPS5063186A (fr)	1975-05-29
CA1034495A (fr)	1978-07-11
NL7413127A (nl)	1975-04-08
SU611595A3 (ru)	1978-06-15

Legal Events

Date	Code	Title	Description
1990-06-29	PL	Patent ceased

Publication	Publication Date	Title
Skeggs Jr et al.	1957	The preparation, purification, and amino acid sequence of a polypeptide renin substrate
Fernandez-Tomas et al.	1973	Morphogenesis of poliovirus II. Demonstration of a new intermediate, the proviron
Hadidian et al.	1948	The preparation and some properties of hyaluronic acid from human umbilical cord
ATE175589T1 (de)	1999-01-15	Verfahren zur formung von partikeln unter verwendung einer überkritischen flüssigkeit so hergestellte aerogelpartikel und aerogelpartikel enthaltenden antiperspirantien
ATE72121T1 (de)	1992-02-15	Verfahren zur herstellung von apolipoproteinen aus blutplasma- oder serumfraktionen.
DE3688310D1 (de)	1993-05-27	Verfahren zur trennung von zucker und konzentrierter schwefelsaeure.
ATE136935T1 (de)	1996-05-15	Verfahren zur gleichzeitigen herstellung von xylit und ethanol
DE60229283D1 (de)	2008-11-20	Kontinuierliches verfahren zur herstellung von ölsaatproteinisolat
SE8204400D0 (sv)	1982-07-20	Forfaringssett for framstellning av porosa cellulosaperlor
PT95830A (pt)	1991-09-30	Processo para o isolamento do factor viii sob a forma de uma fraccao pura directamente de plasma mediante filtracao em gel
SE8402861L (sv)	1985-11-29	Rening av biologiskt material
Senphan et al.	2014	Use of the combined phase partitioning systems for recovery of proteases from hepatopancreas of Pacific white shrimp
CA2314608C (fr)	2013-01-22	Procede d'extraction de fluide d'eclosion produit par du saumon de l'atlantique et d'utilisation dudit fluide
CH617963A5 (fr)	1980-06-30
KR880000465A (ko)	1988-03-26	순수한 알부민의 제조방법
FI922271A0 (fi)	1992-05-19	Foerfarande och komposition foer fram- staellning och anvaendning av ett herpesproteas.
ATE303811T1 (de)	2005-09-15	Herstellung von polygalakturoniden und ihre verwendung als lebensmittelzusatzstoffe
Kunz	1978	Resolution and properties of the proteinases in the larva of the mosquito, Aedes aegypti
KR850003166A (ko)	1985-06-13	B형 간염 비루스 표면항원(HBs 항원)의 정제방법
US9593158B2 (en)	2017-03-14	Process for the preparation of gelatin
DE69841553D1 (de)	2010-04-22	Verfahren zur herstellung von entölten phosphtiden
US3936351A (en)	1976-02-03	Method for preparing glucoronyl-glucosamino-glycan sulphates exhibiting antilipasaemic activity
ATE522544T1 (de)	2011-09-15	Verfahren zur gewinnung und reinigung von rekombinantem protein aus zellen
ATE72756T1 (de)	1992-03-15	Verfahren zur herstellung eines biologischen stoffes.
Graziadei III et al.	1973	Translation of reovirus messenger ribonucleic acids synthesized in vitro into reovirus proteins in a mouse L cell extract