CA3144955A1 - Detection electronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur - Google Patents

Detection electronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur Download PDF

Info

Publication number
CA3144955A1
CA3144955A1 CA3144955A CA3144955A CA3144955A1 CA 3144955 A1 CA3144955 A1 CA 3144955A1 CA 3144955 A CA3144955 A CA 3144955A CA 3144955 A CA3144955 A CA 3144955A CA 3144955 A1 CA3144955 A1 CA 3144955A1
Authority
CA
Canada
Prior art keywords
biologically
channel
reporter
enzyme
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3144955A
Other languages
English (en)
Inventor
Kiana ARAN
Alexander Kane
Brett Goldsmith
Regis Paytavi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Paragraf USA
Original Assignee
Ammr Joint Venture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ammr Joint Venture filed Critical Ammr Joint Venture
Priority claimed from PCT/US2020/039678 external-priority patent/WO2020264204A1/fr
Publication of CA3144955A1 publication Critical patent/CA3144955A1/fr
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6825—Nucleic acid detection involving sensors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/301—Endonuclease

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

L'invention concerne des appareils, des systèmes et des procédés de détection de cible sur la base d'un clivage collatéral d'un rapporteur (730) par une enzyme (720). L'invention porte notamment sur un transistor à grille biologique (106) pouvant comprendre un canal (710) et un fragment rapporteur (730) immobilisé sur le canal (710). L'état de la fraction rapporteur (730) affecte des signaux de sortie provenant du transistor à grille biologique (106) lorsque des conditions d'excitation sont appliquées au transistor à grille biologique (106) et un fluide échantillon (110) est appliqué en contact avec le canal (710). Un fluide échantillon (110) peut comprendre une enzyme (720) configurée pour s'activer en réponse à un acide nucléique cible (722) pour cliver la fraction rapporteur (730). Un circuit d'excitation (1502) applique les conditions d'excitation, et un circuit de mesure (1504) mesure des signaux de sortie provenant du transistor à grille biologique (106). Un module d'analyse (116) détermine un paramètre relatif à la présence de l'acide nucléique cible (722), sur la base de la ou des mesures.
CA3144955A 2019-06-25 2020-06-25 Detection electronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur Pending CA3144955A1 (fr)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201962866312P 2019-06-25 2019-06-25
US62/866,312 2019-06-25
US201916459298A 2019-07-01 2019-07-01
US16/459,298 2019-07-01
US202063036772P 2020-06-09 2020-06-09
US63/036,772 2020-06-09
PCT/US2020/039678 WO2020264204A1 (fr) 2019-06-25 2020-06-25 Détection électronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur

Publications (1)

Publication Number Publication Date
CA3144955A1 true CA3144955A1 (fr) 2020-12-30

Family

ID=79689640

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3144955A Pending CA3144955A1 (fr) 2019-06-25 2020-06-25 Detection electronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur

Country Status (4)

Country Link
EP (1) EP3990630A4 (fr)
KR (1) KR20220024917A (fr)
CN (1) CN114341339A (fr)
CA (1) CA3144955A1 (fr)

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11905552B2 (en) * 2017-08-04 2024-02-20 Keck Graduate Institute Of Applied Life Sciences Immobilized RNPs for sequence-specific nucleic acid capture and digital detection

Also Published As

Publication number Publication date
KR20220024917A (ko) 2022-03-03
EP3990630A4 (fr) 2022-08-17
CN114341339A (zh) 2022-04-12
EP3990630A1 (fr) 2022-05-04

Similar Documents

Publication Publication Date Title
US11561197B2 (en) Electronic detection of a target based on enzymatic cleavage of a reporter moiety
US10345296B2 (en) Device and method for biomarker detection
CN111474365B (zh) 一种生物传感器及制备方法和病毒检测***及方法
CN107690582B (zh) 用于样品分析的装置和方法
Gao et al. Signal-to-noise ratio enhancement of silicon nanowires biosensor with rolling circle amplification
JP6280632B2 (ja) 透明なマイクロアレイで正確にｐＨをモニターするためのデバイスおよび方法
Yang et al. Highly sensitive detection of Staphylococcus aureus by a THz metamaterial biosensor based on gold nanoparticles and rolling circle amplification
US20210382045A1 (en) Dynamic excitation and measurement of biochemical interactions
Cheng et al. A PCR-free point-of-care capacitive immunoassay for influenza A virus
Arya et al. Effects of the electrode size and modification protocol on a label-free electrochemical biosensor
Xu et al. Trigging isothermal circular amplification-based tuning of rigorous fluorescence quenching into complete restoration on a multivalent aptamer probe enables ultrasensitive detection of Salmonella
Wang et al. Aptamer-antibody complementation on multiwalled carbon nanotube-gold transduced dielectrode surfaces to detect pandemic swine influenza virus
US20230107004A1 (en) Reagentless electrochemical biosensor
WO2020264204A1 (fr) Détection électronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur
CA3144955A1 (fr) Detection electronique d'une cible sur la base d'un clivage enzymatique d'une fraction rapporteur
US20210041429A1 (en) Transportation and detection of analytes
US20220365024A1 (en) Integrated circuit with 2d fets for direct and indirect target signal measurement
TWI638049B (zh) 兩階段操作核酸反應檢測管
WO2022246261A2 (fr) Circuit intégré à transistors à effet de champ 2d de mesure directe et indirecte de signal cible
US20190170681A1 (en) Systems and methods for electrical sensing of biomolecular targets
JP2012211819A (ja) バイオセンサ
JP2017209049A (ja) 標的生体分子の特定方法、標的生体分子特定用ビーズ、ビーズのセット、及び標的生体分子特定装置
CN114634974A (zh) 核酸检测***、核酸检测***阵列、核酸检测方法以及候选引导核酸的筛选方法
Esfandyarpour et al. Rapid, label free, high throughput, miniaturized, and inexpensive nanoelectronic array as a cancer diagnosis tool
WO2023091119A1 (fr) Immunocapteur impédimétrique pour le diagnostic de la covid-19