CA2949697A1 - Procede d'edition d'une sequence genetique - Google Patents
Procede d'edition d'une sequence genetique Download PDFInfo
- Publication number
- CA2949697A1 CA2949697A1 CA2949697A CA2949697A CA2949697A1 CA 2949697 A1 CA2949697 A1 CA 2949697A1 CA 2949697 A CA2949697 A CA 2949697A CA 2949697 A CA2949697 A CA 2949697A CA 2949697 A1 CA2949697 A1 CA 2949697A1
- Authority
- CA
- Canada
- Prior art keywords
- sequence
- cell
- fancc
- cells
- donor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/28—Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/06—Antianaemics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
- C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K2035/124—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells the cells being hematopoietic, bone marrow derived or blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Hematology (AREA)
- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Crystallography & Structural Chemistry (AREA)
- Virology (AREA)
- Mycology (AREA)
- Epidemiology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Diabetes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
La présente invention concerne des procédés, des polynucléotides, des cellules, des compositions et des méthodes de traitement qui consistent à modifier une séquence nucléotidique génomique. Généralement, le procédé consiste à introduire, dans une cellule possédant une séquence génomique devant être éditée, le polynucléotide d'un donneur et un nucléotide codant pour une enzyme capable de découper au moins un brin d'ADN, à laisser l'enzyme découper au moins un brin de la séquence génomique et à laisser la séquence du donneur remplacer la séquence génomique devant être éditée.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201462000590P | 2014-05-20 | 2014-05-20 | |
US62/000,590 | 2014-05-20 | ||
PCT/US2015/031807 WO2015179540A1 (fr) | 2014-05-20 | 2015-05-20 | Procédé d'édition d'une séquence génétique |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2949697A1 true CA2949697A1 (fr) | 2015-11-26 |
Family
ID=54554709
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2949697A Abandoned CA2949697A1 (fr) | 2014-05-20 | 2015-05-20 | Procede d'edition d'une sequence genetique |
Country Status (5)
Country | Link |
---|---|
US (1) | US20170175143A1 (fr) |
EP (1) | EP3152221A4 (fr) |
JP (1) | JP2017517256A (fr) |
CA (1) | CA2949697A1 (fr) |
WO (1) | WO2015179540A1 (fr) |
Families Citing this family (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP3613852A3 (fr) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Évaluation et amélioration de la spécificité de clivage des nucléases |
US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
US9068179B1 (en) | 2013-12-12 | 2015-06-30 | President And Fellows Of Harvard College | Methods for correcting presenilin point mutations |
MX2016010285A (es) | 2014-02-11 | 2017-01-11 | Univ Colorado Regents | Ingenieria genetica multiplexada habilitada por crispr. |
AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
EP3294896A1 (fr) * | 2015-05-11 | 2018-03-21 | Editas Medicine, Inc. | Systèmes crispr/cas9 optimisés et procédés d'édition de gènes dans des cellules souches |
AU2016276702B2 (en) | 2015-06-09 | 2022-07-28 | Editas Medicine, Inc. | CRISPR/CAS-related methods and compositions for improving transplantation |
CN108513575A (zh) | 2015-10-23 | 2018-09-07 | 哈佛大学的校长及成员们 | 核碱基编辑器及其用途 |
AU2017280353B2 (en) | 2016-06-24 | 2021-11-11 | Inscripta, Inc. | Methods for generating barcoded combinatorial libraries |
WO2018027078A1 (fr) | 2016-08-03 | 2018-02-08 | President And Fellows Of Harard College | Éditeurs de nucléobases d'adénosine et utilisations associées |
CA3033327A1 (fr) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Proteines de fusion cas9-recombinase programmables et utilisations associees |
WO2018030457A1 (fr) * | 2016-08-10 | 2018-02-15 | 武田薬品工業株式会社 | Procédé de modification d'un site cible dans le génome d'une cellule eucaryote, et procédé de détection de la présence ou de l'absence d'une séquence d'acide nucléique à détecter au niveau d'un site cible |
WO2018039438A1 (fr) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation d'acides aminés non naturels dans des protéines au moyen de l'édition de bases |
KR20240007715A (ko) | 2016-10-14 | 2024-01-16 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
KR101997116B1 (ko) | 2016-10-14 | 2019-07-05 | 연세대학교 산학협력단 | Kras 유전자에 상보적인 가이드 rna 및 이의 용도 |
WO2018070850A1 (fr) * | 2016-10-14 | 2018-04-19 | 연세대학교 산학협력단 | Arn guide complémentaire du gène kras, et son utilisation |
US10745677B2 (en) | 2016-12-23 | 2020-08-18 | President And Fellows Of Harvard College | Editing of CCR5 receptor gene to protect against HIV infection |
TW201839136A (zh) | 2017-02-06 | 2018-11-01 | 瑞士商諾華公司 | 治療血色素異常症之組合物及方法 |
EP3592853A1 (fr) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression de la douleur par édition de gène |
JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
SG11201908658TA (en) | 2017-03-23 | 2019-10-30 | Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
EP3652312A1 (fr) | 2017-07-14 | 2020-05-20 | Editas Medicine, Inc. | Systèmes et procédés d'intégration ciblée et d'édition du génome et détection de celle-ci à l'aide de sites d'amorçage intégrés |
US11732274B2 (en) | 2017-07-28 | 2023-08-22 | President And Fellows Of Harvard College | Methods and compositions for evolving base editors using phage-assisted continuous evolution (PACE) |
MX2020001177A (es) | 2017-07-31 | 2020-09-25 | Regeneron Pharma | Animales no humanos reporteros de crispr y usos de los mismos. |
SG11201912235PA (en) | 2017-07-31 | 2020-01-30 | Regeneron Pharma | Cas-transgenic mouse embryonic stem cells and mice and uses thereof |
US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
CN111757937A (zh) | 2017-10-16 | 2020-10-09 | 布罗德研究所股份有限公司 | 腺苷碱基编辑器的用途 |
BR112020020841A2 (pt) * | 2018-04-11 | 2021-01-19 | Fundacion Para La Investigacion Biomedica Del Hospital Infantil Universitario Nino Jesus | Composições e métodos para transplante de célulastronco |
CN113454235A (zh) * | 2019-02-21 | 2021-09-28 | 豪夫迈·罗氏有限公司 | 经改进的核酸靶标富集和相关方法 |
DE112020001342T5 (de) | 2019-03-19 | 2022-01-13 | President and Fellows of Harvard College | Verfahren und Zusammensetzungen zum Editing von Nukleotidsequenzen |
EP4146804A1 (fr) | 2020-05-08 | 2023-03-15 | The Broad Institute Inc. | Méthodes et compositions d'édition simultanée des deux brins d'une séquence nucléotidique double brin cible |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20060228798A1 (en) * | 2002-11-27 | 2006-10-12 | Catherine Verfaillie | Homologous recombination in multipotent adult progenitor cells |
DK3401400T3 (da) * | 2012-05-25 | 2019-06-03 | Univ California | Fremgangsmåder og sammensætninger til rna-styret mål-dna-modifikation og til rna-styret transskriptionsmodulering |
EP3763810A3 (fr) * | 2012-10-10 | 2021-07-14 | Sangamo Therapeutics, Inc. | Composés de modification de cellules t et leurs utilisations |
CN110643600A (zh) * | 2012-10-23 | 2020-01-03 | 基因工具股份有限公司 | 用于切割靶dna的***及其用途 |
-
2015
- 2015-05-20 EP EP15796528.6A patent/EP3152221A4/fr not_active Withdrawn
- 2015-05-20 CA CA2949697A patent/CA2949697A1/fr not_active Abandoned
- 2015-05-20 US US15/311,685 patent/US20170175143A1/en not_active Abandoned
- 2015-05-20 JP JP2016568592A patent/JP2017517256A/ja active Pending
- 2015-05-20 WO PCT/US2015/031807 patent/WO2015179540A1/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
JP2017517256A (ja) | 2017-06-29 |
US20170175143A1 (en) | 2017-06-22 |
WO2015179540A1 (fr) | 2015-11-26 |
EP3152221A1 (fr) | 2017-04-12 |
EP3152221A4 (fr) | 2018-01-24 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FZDE | Discontinued |
Effective date: 20211123 |
|
FZDE | Discontinued |
Effective date: 20211123 |