CA2799883C - Procede ameliorant la separation d'une solution organique hydrophobe d'un milieu de culture aqueux - Google Patents
Procede ameliorant la separation d'une solution organique hydrophobe d'un milieu de culture aqueux Download PDFInfo
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- CA2799883C CA2799883C CA2799883A CA2799883A CA2799883C CA 2799883 C CA2799883 C CA 2799883C CA 2799883 A CA2799883 A CA 2799883A CA 2799883 A CA2799883 A CA 2799883A CA 2799883 C CA2799883 C CA 2799883C
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6436—Fatty acid esters
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/001—Oxidoreductases (1.) acting on the CH-CH group of donors (1.3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y103/00—Oxidoreductases acting on the CH-CH group of donors (1.3)
- C12Y103/99—Oxidoreductases acting on the CH-CH group of donors (1.3) with other acceptors (1.3.99)
- C12Y103/99003—Acyl-CoA dehydrogenase (1.3.99.3)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
La présente invention concerne un procédé de séparation améliorée dune solution organique hydrophobe dun milieu de culture aqueux, le procédé comprenant les étapes suivantes : préparation dun milieu de culture aqueux comprenant une cellule métaboliquement active, mise en contact du milieu de culture aqueux avec une solution organique hydrophobe, et séparation de la solution organique hydrophobe du milieu de culture aqueux, la cellule présentant une activité, réduite par rapport à son type sauvage, dau moins un enzyme qui catalyse une des réactions de ß oxydation dacides gras. Linvention concerne également lutilisation dune cellule métaboliquement active qui présente une activité, réduite par rapport à son type sauvage, dun enzyme qui catalyse une des réactions de ß oxydation dacides gras, de préférence dun enzyme sélectionné dans le groupe consistant en FadA, FadB, FadD, FadL et FadE ainsi que des variantes de ceux-ci, de préférence FadE ou une variante de celui-ci, pour la séparation améliorée dune solution organique hydrophobe dun milieu de culture aqueux comprenant la cellule métaboliquement active.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP11195221.4A EP2607490A1 (fr) | 2011-12-22 | 2011-12-22 | Procédé de séparation améliorée d'une solution organique hydrophobe et d'un milieu de culture aqueux |
| EP11195221 | 2011-12-22 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2799883A1 CA2799883A1 (fr) | 2013-06-22 |
| CA2799883C true CA2799883C (fr) | 2020-09-22 |
Family
ID=47351527
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2799883A Active CA2799883C (fr) | 2011-12-22 | 2012-12-20 | Procede ameliorant la separation d'une solution organique hydrophobe d'un milieu de culture aqueux |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9249435B2 (fr) |
| EP (3) | EP2607490A1 (fr) |
| JP (1) | JP6312117B2 (fr) |
| KR (1) | KR102000317B1 (fr) |
| CN (2) | CN103254089A (fr) |
| BR (1) | BR112014015202B1 (fr) |
| CA (1) | CA2799883C (fr) |
| ES (1) | ES2688295T3 (fr) |
| MX (1) | MX339910B (fr) |
| SG (1) | SG11201403495QA (fr) |
| WO (1) | WO2013092547A1 (fr) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102006025821A1 (de) | 2006-06-02 | 2007-12-06 | Degussa Gmbh | Ein Enzym zur Herstellung von Mehylmalonatsemialdehyd oder Malonatsemialdehyd |
| UA112980C2 (uk) | 2011-02-16 | 2016-11-25 | Евонік Дегусса Гмбх | Рідкі катіоніти |
| CA2832624A1 (fr) | 2011-04-12 | 2012-10-18 | Evonik Degussa Gmbh | Procede pouvant etre mis en oeuvre en continu, pour la production de composes carbonyle, au moyen d'un catalyseur contenant des radicaux nitrosyle |
| EP2540170A1 (fr) | 2011-06-29 | 2013-01-02 | Evonik Degussa GmbH | Extrait de levure à usage dermatologique |
| BR112014000947A2 (pt) | 2011-07-20 | 2017-06-13 | Evonik Degussa Gmbh | oxidação e aminação de álcoois primários |
| EP2602328A1 (fr) | 2011-12-05 | 2013-06-12 | Evonik Industries AG | Procédé d'oxydation d'alcanes en utilisant d'une alcane 1-mono-oxygénase AlkB |
| EP2607479A1 (fr) | 2011-12-22 | 2013-06-26 | Evonik Industries AG | Production bio-technologique d'alcools et dérivés associés |
| EP2631298A1 (fr) | 2012-02-22 | 2013-08-28 | Evonik Industries AG | Procédé biotechnologique et procédé de production de butanol et d'acide butyrique |
| EP2639308A1 (fr) | 2012-03-12 | 2013-09-18 | Evonik Industries AG | Omega-oxydation et -amination enzymatique d'acides gras |
| EP2647696A1 (fr) | 2012-04-02 | 2013-10-09 | Evonik Degussa GmbH | Procédé de fabrication aérobie d'alanine ou d'une liaison établie sous consommation d'alanine |
| DE102012207921A1 (de) | 2012-05-11 | 2013-11-14 | Evonik Industries Ag | Mehrstufiges Syntheseverfahren mit Synthesegas |
| EP2700448A1 (fr) | 2012-08-21 | 2014-02-26 | Evonik Industries AG | Acides gras ramifiés comme échangeurs de cations liquides |
| EP2706076B1 (fr) | 2012-09-07 | 2014-12-17 | Evonik Industries AG | Compositions durcissables à base de résines époxy sans alcool benzylique |
| EP2730655A1 (fr) | 2012-11-12 | 2014-05-14 | Evonik Industries AG | Procédé de conversion d'un ester d'acide carboxylique en utilisant des cellules déficientes en BioH |
| EP2746400A1 (fr) | 2012-12-21 | 2014-06-25 | Evonik Industries AG | Fabrication d'amines et de diamines à partir d'acide carbonique ou d'acide de dicarbone ou un mono-ester composé de ceux-ci |
| EP2746397A1 (fr) | 2012-12-21 | 2014-06-25 | Evonik Industries AG | Fabrication d'acides gras oméga-aminés |
| EP2759598A1 (fr) | 2013-01-24 | 2014-07-30 | Evonik Industries AG | Procédé de fabrication d'alpha, oméga-alcane-diol |
| EP2944697A1 (fr) | 2014-05-13 | 2015-11-18 | Evonik Degussa GmbH | Procédé de production de nylon |
| CN108473967B (zh) | 2015-12-17 | 2022-03-29 | 赢创运营有限公司 | 基因修饰的产乙酸细胞 |
| EP3336180A1 (fr) | 2016-07-04 | 2018-06-20 | Evonik Degussa GmbH | Gène alkb mutante |
| WO2018019867A1 (fr) | 2016-07-27 | 2018-02-01 | Evonik Degussa Gmbh | N-acétyl homosérine |
| CN114174309A (zh) * | 2019-07-29 | 2022-03-11 | 赢创运营有限公司 | 从水性培养基中萃取碳酸、脂族酸、酯和醇的方法 |
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| US6245214B1 (en) | 1998-09-18 | 2001-06-12 | Alliedsignal Inc. | Electro-catalytic oxidation (ECO) device to remove CO from reformate for fuel cell application |
| DE10054347A1 (de) | 2000-11-02 | 2002-05-08 | Degussa | Verfahren zur katalytischen Hydrierung organischer Verbindungen und Trägerkatalysatoren hierfür |
| DE10142621A1 (de) | 2001-08-31 | 2003-03-20 | Degussa | Aufarbeitung der Ammoximationsprodukte von Ketonen durch Flüssig-Flüssig-Extraktion in einem ternären Lösemittelsystem |
| DE10142620A1 (de) | 2001-08-31 | 2003-03-20 | Degussa | Ammoximation von Ketonen und Aufarbeitung durch Pervaporation/Dampfpermeation |
| ES2322033T3 (es) | 2002-05-31 | 2009-06-16 | Evonik Degussa Gmbh | Catalizador de rutenio soportado y procedimiento para la hidrogenacion de una amina aromatica en presencia de este catalizador. |
| DE10231119A1 (de) | 2002-07-10 | 2004-02-05 | Degussa Ag | Verfahren zur Selektivitätserhöhung der Hydrierung von 4,4'-Diaminodiphenylmethan zu 4,4'-Diaminodicyclohexylmethan in Gegenwart eines N-Alkyl-4,4'-Diaminodiphenylmethans |
| DE10247495A1 (de) | 2002-10-11 | 2004-04-22 | Degussa Ag | Verfahren zur Epoxidierung cyclischer Alkene |
| EP1424332A1 (fr) | 2002-11-26 | 2004-06-02 | Degussa AG | Procédé de purification de l'oxyde de propène brut |
| US6878836B2 (en) | 2003-06-18 | 2005-04-12 | Degussa Ag | Process for the epoxidation of propene |
| DE102006017760A1 (de) | 2006-03-24 | 2007-09-27 | Ufz-Umweltforschungszentrum Leipzig-Halle Gmbh | Verfahren zur enzymatischen Herstellung von 2-Hydroxy-2-methylcarbonsäuren |
| EP2024501B1 (fr) | 2006-05-11 | 2016-11-16 | Evonik Degussa GmbH | Production améliorée de bases sphingoides à l'aide de souches microbiennes modifiées par voie génétique |
| DE102006025821A1 (de) | 2006-06-02 | 2007-12-06 | Degussa Gmbh | Ein Enzym zur Herstellung von Mehylmalonatsemialdehyd oder Malonatsemialdehyd |
| DE102007021199B4 (de) | 2006-07-17 | 2016-02-11 | Evonik Degussa Gmbh | Zusammensetzungen aus organischem Polymer als Matrix und anorganischen Partikeln als Füllstoff, Verfahren zu deren Herstellung sowie deren Verwendung und damit hergestellte Formkörper |
| DE102007027006A1 (de) | 2007-06-08 | 2008-12-11 | Evonik Degussa Gmbh | Mikrobiologische Herstellung von Aldehyden, insbesondere von 3-Hydroxypropionaldehyd |
| DE102007052463A1 (de) | 2007-11-02 | 2009-05-07 | Evonik Degussa Gmbh | Fermentative Gewinnung von Aceton aus erneuerbaren Rohstoffen mittels neuen Stoffwechselweges |
| DE102007060705A1 (de) | 2007-12-17 | 2009-06-18 | Evonik Degussa Gmbh | ω-Aminocarbonsäuren oder ihre Lactame, herstellende, rekombinante Zellen |
| US8445221B2 (en) * | 2007-12-28 | 2013-05-21 | Ikeda Food Research Co., Ltd. | Modified glucose dehydrogenase gene |
| DE102008002090A1 (de) | 2008-05-30 | 2009-12-03 | Evonik Degussa Gmbh | Ungesättigte Dicarbonsäuren aus ungesättigten cyclischen Kohlenwasserstoffen und Acrylsäure mittels Metathese, deren Verwendung als Monomere für Polyamide, Polyester, Polyurethane sowie weitere Umsetzung zu DIolen und Diaminen |
| DE102008002715A1 (de) | 2008-06-27 | 2009-12-31 | Evonik Röhm Gmbh | 2-Hydroxyisobuttersäure produzierende rekombinante Zelle |
| DE102008040193A1 (de) | 2008-07-04 | 2010-01-07 | Evonik Röhm Gmbh | Verfahren zur Herstellung freier Carbonsäuren |
| DE102008040415A1 (de) | 2008-07-15 | 2010-01-21 | Evonik Röhm Gmbh | Thermisches Salzspalten von Ammoniumcarboxylaten |
| WO2010021711A1 (fr) * | 2008-08-18 | 2010-02-25 | Ls9, Inc. | Systèmes et procédés de production d'esters gras mixtes |
| DE102009000592A1 (de) | 2009-02-04 | 2010-08-05 | Evonik Degussa Gmbh | Verfahren zur Herstellung von Aminogruppen tragenden, multizyklischen Ringsystemen |
| DE102009000661A1 (de) | 2009-02-06 | 2010-08-12 | Evonik Degussa Gmbh | Verfahren zur Herstellung von 2,6-Dioxabicyclo-(3.3.0)-octan-4,8-dion[1S,5S] |
| DE102009000662A1 (de) | 2009-02-06 | 2010-08-12 | Evonik Degussa Gmbh | Verfahren zur Herstellung von Aldehyden und Ketonen aus primären und sekundären Alkoholen |
| DE102009009580A1 (de) | 2009-02-19 | 2010-08-26 | Evonik Degussa Gmbh | Verfahren zur Herstellung freier Säuren aus ihren Salzen |
| DE102009002371A1 (de) | 2009-04-15 | 2010-10-21 | Evonik Goldschmidt Gmbh | Verfahren zur Herstellung von geruchlosen Polyetheralkoholen mittels DMC-Katalysatoren und deren Verwendung in kosmetischen und/oder dermatologischen Zubereitungen |
| BRPI1015313B1 (pt) * | 2009-04-27 | 2021-06-01 | Genomatica, Inc. | Método fermentativo para produzir um éster de ácido graxo empregando um microorganismo geneticamente modificado e método para a produção de ésteres de ácidos graxos |
| DE102009002811A1 (de) | 2009-05-05 | 2010-11-11 | Evonik Degussa Gmbh | Enzymatisches Verfahren zur Herstellung von Aldehyden |
| DE102009046626A1 (de) | 2009-11-11 | 2011-05-12 | Evonik Degussa Gmbh | Candida tropicalis Zellen und deren Verwendung |
| DE102009046910A1 (de) | 2009-11-20 | 2011-05-26 | Evonik Degussa Gmbh | Verfahren zur Aufarbeitung eines Laurinlactam enthaltenen Stoffstroms für die Rückgewinnung aller enthaltene Wertstoffkomponenten durch Kombination von Kristallisation mit nachgeschalteter Destillation |
| AU2010335313C1 (en) | 2009-12-23 | 2015-07-02 | Evonik Operations Gmbh | Sweetener and method for the production thereof |
| DE102010015807A1 (de) | 2010-04-20 | 2011-10-20 | Evonik Degussa Gmbh | Biokatalytisches Oxidationsverfahren mit alkL-Genprodukt |
| WO2011157573A2 (fr) | 2010-06-14 | 2011-12-22 | Evonik Röhm Gmbh | Enzyme permettant de produire du méthylmalonate-semi-aldéhyde |
| DE102010023749A1 (de) | 2010-06-14 | 2011-12-15 | Evonik Degussa Gmbh | Zelle und Verfahren zur Herstellung von Resveratrol |
| DE102010026196A1 (de) | 2010-06-25 | 2011-12-29 | Evonik Degussa Gmbh | Synthese von omega-Aminocarbonsäuren und deren Estern aus ungesättigten Fettsäurederivaten |
| UA112980C2 (uk) | 2011-02-16 | 2016-11-25 | Евонік Дегусса Гмбх | Рідкі катіоніти |
| DE102011015150A1 (de) | 2011-03-25 | 2012-09-27 | Evonik Degussa Gmbh | Syntese von alpha, omega-Dicarbonsäuren und deren Estern aus ungesättigten Fettsäurederivaten |
| CA2832624A1 (fr) | 2011-04-12 | 2012-10-18 | Evonik Degussa Gmbh | Procede pouvant etre mis en oeuvre en continu, pour la production de composes carbonyle, au moyen d'un catalyseur contenant des radicaux nitrosyle |
| EP2557176A1 (fr) | 2011-06-15 | 2013-02-13 | Evonik Degussa GmbH | Amination enzymatique |
| BR112014000947A2 (pt) | 2011-07-20 | 2017-06-13 | Evonik Degussa Gmbh | oxidação e aminação de álcoois primários |
| CN107034247A (zh) | 2011-08-05 | 2017-08-11 | 赢创德固赛有限公司 | 仲醇的氧化和胺化 |
| DE102011110946A1 (de) | 2011-08-15 | 2016-01-21 | Evonik Degussa Gmbh | Biotechnologisches Syntheseverfahren von omegafunktionalisierten Carbonsäuren und Carbonsäure-Estern aus einfachen Kohlenstoffquellen |
| DE102011110945A1 (de) | 2011-08-15 | 2013-02-21 | Evonik Degussa Gmbh | Biotechnologisches Syntheseverfahren von organischen Verbindungen mit alkIL-Genprodukt |
| EP2730655A1 (fr) | 2012-11-12 | 2014-05-14 | Evonik Industries AG | Procédé de conversion d'un ester d'acide carboxylique en utilisant des cellules déficientes en BioH |
| EP2746397A1 (fr) | 2012-12-21 | 2014-06-25 | Evonik Industries AG | Fabrication d'acides gras oméga-aminés |
| DE102013203470A1 (de) | 2013-03-01 | 2014-09-04 | Evonik Industries Ag | Verfahren zur Herstellung von Ketonen aus Epoxiden |
-
2011
- 2011-12-22 EP EP11195221.4A patent/EP2607490A1/fr not_active Withdrawn
-
2012
- 2012-12-18 ES ES12806038.1T patent/ES2688295T3/es active Active
- 2012-12-18 SG SG11201403495QA patent/SG11201403495QA/en unknown
- 2012-12-18 EP EP12806038.1A patent/EP2794898B1/fr active Active
- 2012-12-18 MX MX2012015064A patent/MX339910B/es active IP Right Grant
- 2012-12-18 WO PCT/EP2012/075889 patent/WO2013092547A1/fr active Application Filing
- 2012-12-18 EP EP12197644.3A patent/EP2607491A1/fr not_active Withdrawn
- 2012-12-18 KR KR1020147016716A patent/KR102000317B1/ko active IP Right Grant
- 2012-12-18 BR BR112014015202-0A patent/BR112014015202B1/pt active IP Right Grant
- 2012-12-20 CA CA2799883A patent/CA2799883C/fr active Active
- 2012-12-20 US US13/721,481 patent/US9249435B2/en active Active
- 2012-12-21 CN CN2012105994118A patent/CN103254089A/zh active Pending
- 2012-12-21 CN CN201810198137.0A patent/CN108358800B/zh active Active
- 2012-12-25 JP JP2012281367A patent/JP6312117B2/ja active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN108358800B (zh) | 2023-02-17 |
| EP2794898A1 (fr) | 2014-10-29 |
| CN108358800A (zh) | 2018-08-03 |
| EP2607491A1 (fr) | 2013-06-26 |
| US9249435B2 (en) | 2016-02-02 |
| BR112014015202A8 (pt) | 2017-06-13 |
| CA2799883A1 (fr) | 2013-06-22 |
| BR112014015202A2 (pt) | 2017-06-13 |
| JP2013132295A (ja) | 2013-07-08 |
| EP2607490A1 (fr) | 2013-06-26 |
| WO2013092547A1 (fr) | 2013-06-27 |
| EP2794898B1 (fr) | 2018-08-01 |
| KR20140111653A (ko) | 2014-09-19 |
| CN103254089A (zh) | 2013-08-21 |
| JP6312117B2 (ja) | 2018-04-18 |
| US20130164797A1 (en) | 2013-06-27 |
| MX339910B (es) | 2016-06-15 |
| SG11201403495QA (en) | 2014-09-26 |
| ES2688295T3 (es) | 2018-10-31 |
| MX2012015064A (es) | 2013-10-16 |
| BR112014015202B1 (pt) | 2021-11-03 |
| KR102000317B1 (ko) | 2019-07-15 |
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| EEER | Examination request |
Effective date: 20171201 |