CA2790952C - Compounds as bradykinin b1 antagonists - Google Patents
Compounds as bradykinin b1 antagonists Download PDFInfo
- Publication number
- CA2790952C CA2790952C CA2790952A CA2790952A CA2790952C CA 2790952 C CA2790952 C CA 2790952C CA 2790952 A CA2790952 A CA 2790952A CA 2790952 A CA2790952 A CA 2790952A CA 2790952 C CA2790952 C CA 2790952C
- Authority
- CA
- Canada
- Prior art keywords
- denotes
- compounds
- compound
- general formula
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
- C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
- C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
- C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
- C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/495—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with two or more nitrogen atoms as the only ring heteroatoms, e.g. piperazine or tetrazines
- A61K31/50—Pyridazines; Hydrogenated pyridazines
- A61K31/501—Pyridazines; Hydrogenated pyridazines not condensed and containing further heterocyclic rings
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P19/00—Drugs for skeletal disorders
- A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/04—Centrally acting analgesics, e.g. opioids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/06—Antimigraine agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C211/00—Compounds containing amino groups bound to a carbon skeleton
- C07C211/43—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton
- C07C211/54—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton having amino groups bound to two or three six-membered aromatic rings
- C07C211/56—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to carbon atoms of six-membered aromatic rings of the carbon skeleton having amino groups bound to two or three six-membered aromatic rings the carbon skeleton being further substituted by halogen atoms or by nitro or nitroso groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D213/00—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members
- C07D213/02—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members
- C07D213/04—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom
- C07D213/60—Heterocyclic compounds containing six-membered rings, not condensed with other rings, with one nitrogen atom as the only ring hetero atom and three or more double bonds between ring members or between ring members and non-ring members having three double bonds between ring members or between ring members and non-ring members having no bond between the ring nitrogen atom and a non-ring member or having only hydrogen or carbon atoms directly attached to the ring nitrogen atom with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D213/72—Nitrogen atoms
- C07D213/74—Amino or imino radicals substituted by hydrocarbon or substituted hydrocarbon radicals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D237/00—Heterocyclic compounds containing 1,2-diazine or hydrogenated 1,2-diazine rings
- C07D237/02—Heterocyclic compounds containing 1,2-diazine or hydrogenated 1,2-diazine rings not condensed with other rings
- C07D237/06—Heterocyclic compounds containing 1,2-diazine or hydrogenated 1,2-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
- C07D237/10—Heterocyclic compounds containing 1,2-diazine or hydrogenated 1,2-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D237/24—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D307/00—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom
- C07D307/02—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings
- C07D307/04—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having no double bonds between ring members or between ring members and non-ring members
- C07D307/18—Heterocyclic compounds containing five-membered rings having one oxygen atom as the only ring hetero atom not condensed with other rings having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D307/24—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Pain & Pain Management (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Biomedical Technology (AREA)
- Rheumatology (AREA)
- Immunology (AREA)
- Orthopedic Medicine & Surgery (AREA)
- Physical Education & Sports Medicine (AREA)
- Epidemiology (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Plural Heterocyclic Compounds (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
Abstract
The present invention relates to the compounds of general formula I
(see formula I) wherein n, R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11 and X are defined as described hereinafter, the enantiomers, the diastereomers, the mixtures and the salts thereof, particularly the physiologically acceptable salts thereof with organic or inorganic acids or bases, which have valuable properties, the preparation thereof, the pharmaceutical formulations containing the pharmacologically effective compounds, the preparation thereof and the use thereof.
(see formula I) wherein n, R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, R11 and X are defined as described hereinafter, the enantiomers, the diastereomers, the mixtures and the salts thereof, particularly the physiologically acceptable salts thereof with organic or inorganic acids or bases, which have valuable properties, the preparation thereof, the pharmaceutical formulations containing the pharmacologically effective compounds, the preparation thereof and the use thereof.
Description
The present invention relates to the compounds of general formula I
RI2 0 Rs Rs RtNX
R7 el Rs o R3/ \R42 , (I) wherein n, R1, R2, R3, R4, R5, R6, R7, R8, R9, K=-=10, R" and X are as defined hereinafter, the enantiomers, the diastereomers, the mixtures and the salts thereof, particularly the physiologically acceptable salts thereof with organic or inorganic acids or bases, which have io valuable properties, the preparation thereof, the pharmaceutical formulations containing the pharmacologically effective compounds, the preparation thereof and the use thereof.
BACKGROUND TO THE INVENTION
TECHNICAL FIELD
The present invention relates to 3-oxo-pyridazine compounds and their use as B1-receptor antagonists, and pharmaceutical formulations containing these compounds.
PRIOR ART
Compounds with a 81-antagonistic activity have already been described in International Patent Application PCT/EP2010/052232 or in the priority application on which it is based.
One aim of the present invention was to provide new compounds which may be suitable in particular as pharmaceutical active substances that can be used for the treatment of diseases at least partly mediated by the B1 receptor, such as pain or inflammation (see "Bradykinin antagonists: new opportunities", Bock M. G. and Longmou J., Carr.
Opin. Chem.
Biol. 200 Aug; 464: 401-6).
RI2 0 Rs Rs RtNX
R7 el Rs o R3/ \R42 , (I) wherein n, R1, R2, R3, R4, R5, R6, R7, R8, R9, K=-=10, R" and X are as defined hereinafter, the enantiomers, the diastereomers, the mixtures and the salts thereof, particularly the physiologically acceptable salts thereof with organic or inorganic acids or bases, which have io valuable properties, the preparation thereof, the pharmaceutical formulations containing the pharmacologically effective compounds, the preparation thereof and the use thereof.
BACKGROUND TO THE INVENTION
TECHNICAL FIELD
The present invention relates to 3-oxo-pyridazine compounds and their use as B1-receptor antagonists, and pharmaceutical formulations containing these compounds.
PRIOR ART
Compounds with a 81-antagonistic activity have already been described in International Patent Application PCT/EP2010/052232 or in the priority application on which it is based.
One aim of the present invention was to provide new compounds which may be suitable in particular as pharmaceutical active substances that can be used for the treatment of diseases at least partly mediated by the B1 receptor, such as pain or inflammation (see "Bradykinin antagonists: new opportunities", Bock M. G. and Longmou J., Carr.
Opin. Chem.
Biol. 200 Aug; 464: 401-6).
- 2 -An essential structural feature of the new compounds is the 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid amide group that is present in tautomeric equilibrium with the 6-hydroxy-pyridazine-4-carboxylic acid amide group:
,rµt, N H
FIN H
Compared with the compounds from the prior art, the new substances are characterised in that they exhibit a strong BI-receptor blocking activity and at the same time have improved metabolic stability.
DETAILED DESCRIPTION OF THE INVENTION
In the above general formula I in one embodiment 1 HN
R1 denotes the group R2 denotes H or CH3, R3 and R4 together with the carbon atom to which they are bound denote a Cm-cycloalkylene group wherein a -CH2 unit may be replaced by an oxygen atom, Rs denotes H or CH3, R6 denotes H, F, Cl or methyl, R7 denotes H, F, Cl, Br, -CN, C1.4-alkyl, CF3, CHF2, R8 denotes H, R9 denotes F, Cl, Br, C1_4-alkyl, -0-C1_4-alkyl, -S-C1_4-alkyl,
,rµt, N H
FIN H
Compared with the compounds from the prior art, the new substances are characterised in that they exhibit a strong BI-receptor blocking activity and at the same time have improved metabolic stability.
DETAILED DESCRIPTION OF THE INVENTION
In the above general formula I in one embodiment 1 HN
R1 denotes the group R2 denotes H or CH3, R3 and R4 together with the carbon atom to which they are bound denote a Cm-cycloalkylene group wherein a -CH2 unit may be replaced by an oxygen atom, Rs denotes H or CH3, R6 denotes H, F, Cl or methyl, R7 denotes H, F, Cl, Br, -CN, C1.4-alkyl, CF3, CHF2, R8 denotes H, R9 denotes F, Cl, Br, C1_4-alkyl, -0-C1_4-alkyl, -S-C1_4-alkyl,
- 3 -R10 denotes H, R11 denotes F, Cl, Br, -ON, C1.4-alkyl, CF3, CHF2, and X denotes CH or N, the enantiomers, the diastereomers, the mixtures and the salts thereof, particularly the physiologically acceptable salts thereof with organic or inorganic acids or bases.
An embodiment 2 of the present invention comprises the compounds of general formula I, wherein n, R1, R3, R4, Rs, R6, R7, R8, R9, R10, R11 and X are defined as described hereinbefore in embodiment 1 and R2 denotes H, the enantiomers, the diastereomers, the mixtures and the salts thereof, particularly the physiologically acceptable salts thereof with organic or inorganic acids or bases.
The following are mentioned for example as most particularly preferred compounds of the above general formula I:
No. Structure ,N, io(1) 0 H I
F F
ao(2) 0 H
F F
An embodiment 2 of the present invention comprises the compounds of general formula I, wherein n, R1, R3, R4, Rs, R6, R7, R8, R9, R10, R11 and X are defined as described hereinbefore in embodiment 1 and R2 denotes H, the enantiomers, the diastereomers, the mixtures and the salts thereof, particularly the physiologically acceptable salts thereof with organic or inorganic acids or bases.
The following are mentioned for example as most particularly preferred compounds of the above general formula I:
No. Structure ,N, io(1) 0 H I
F F
ao(2) 0 H
F F
- 4 -No. Structure J.....)1, * :-,0 0 ' NJ
(3) N* F
o H r F F
(4) = -1i, EN1o;l,N O 0 CI
H r F F
HN
õN.,
(3) N* F
o H r F F
(4) = -1i, EN1o;l,N O 0 CI
H r F F
HN
õN.,
(5) o H
F F
F
,N, HN --z- H 0 F
O ' N *
F F
F
,N, HN --z- H 0 F
O ' N *
(6) 0 H 0 H
F F
F
,N, HN -, w 0 ji-Ndilk CI
O ' N
F F
F
,N, HN -, w 0 ji-Ndilk CI
O ' N
(7) 0 o H * *
H
F F
F
HN,N,, ..,,,,..yo=li, Br 0 = N *N 0
H
F F
F
HN,N,, ..,,,,..yo=li, Br 0 = N *N 0
(8) 0 H
H
F F
F
HN,Nrl 0 / INI,
H
F F
F
HN,Nrl 0 / INI,
(9) o i H
F F F
the enantiomers, the diastereomers, the mixtures and the salts thereof, particularly the physiologically acceptable salts thereof with organic or inorganic acids or bases.
In another aspect of the invention, a pharmaceutical formulation comprising a compound as described herein and one or more inert carriers and/or diluents is provided.
TERMS AND DEFINITIONS USED
Unless otherwise stated, all the substituents are independent of one another.
If for example io there are a plurality of C1_4-alkyl groups as substituents in one group, in the case of three substituents C1_4-alkyl, one may represent methyl, one n-propyl and one tert-butyl.
Within the scope of this application, in the definition of possible substituents, these may also be represented in the form of a structural formula. If present, an asterisk (*) in the structural formula of the substituent is to be understood as being the linking point to the rest of the molecule.
Also included in the subject matter of this invention are the compounds according to the invention, including the salts thereof, in which one or more hydrogen atoms, for example one, two, three, four or five hydrogen atoms, are replaced by deuterium.
By the term "C1_4-alkyl" (including those that are part of other groups) are meant alkyl groups with 1 to 4 carbon atoms. Examples include: methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl and tert-butyl. The abbreviations Me, Et, n-Pr, i-Pr, n-Bu, i-Bu, t-Bu, etc. may optionally also be used for the above-mentioned groups. Unless stated otherwise, the definitions propyl and butyl include all the possible isomeric forms of the groups in question.
Thus, for example, propyl includes n-propyl and iso-propyl, butyl includes iso-butyl, sec-butyl and tert-butyl.
Moreover the definitions mentioned previously also include those groups wherein each methylene group may be substituted by up to two and each methyl group may be substituted by up to three fluorine atoms.
By the term "Cm-cycloalkyl" (including those that are part of other groups) are meant cyclic alkyl groups with 3 to 6 carbon atoms. Examples include: cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl.
If they contain suitable basic functions, for example amino groups, compounds of general formula I may be converted, particularly for pharmaceutical use, into the physiologically acceptable salts thereof with inorganic or organic acids. Examples of inorganic acids for this purpose include hydrobromic acid, phosphoric acid, nitric acid, hydrochloric acid, sulphuric acid, methanesulphonic acid, ethanesulphonic acid, benzenesulphonic acid or p-toluenesulphonic acid, while organic acids that may be used include malic acid, succinic acid, acetic acid, fumaric acid, maleic acid, mandelic acid, lactic acid, tartaric acid or citric acid.
In addition, the compounds of general formula I, if they contain suitable carboxylic acid functions, may be converted into the physiologically acceptable salts thereof with inorganic or organic bases, particularly for pharmaceutical applications. Examples of inorganic bases include alkali or alkaline earth metal hydroxides, e.g. sodium hydroxide or potassium hydroxide, or carbonates, ammonia, zinc or ammonium hydroxides; examples of organic amines include diethylamine, triethylamine, ethanolamine, diethanolamine, triethanolamine, cyclohexylamine or dicyclohexylamine.
The compounds according to the invention may be present as racemates, provided that they have only one chiral element, but may also be obtained as pure enantiomers, i.e. In the (R) Or (S) form.
However, the application also includes the individual diastereomeric pairs of antipodes or mixtures thereof, which are obtained if there is more than one chiral element in the compounds of general formula I, as well as the individual optically active enantiomers of which the above-mentioned racemates are made up.
Compounds with a carbon double bond may be present in both the E and Z form.
=
If a compound is present in different tautomeric forms, the compound prepared is not limited to one tautomeric form but includes all the tautomeric forms. This also applies particularly to nitrogen-containing heteroaryls:
-N OH _____________________________________ N-N 0 PREPARATION METHODS
According to the invention the compounds of general formula I are obtained by methods io known per se, for example by the following methods:
(A) amide coupling:
RIO
( I )0 R3 R4 (R6)õ Ir R11 (II) (III) The linking of carboxylic acids of general formula II as shown, wherein all the groups are as hereinbefore defined, with amines of general formula III, wherein all the groups are as hereinbefore defined, to form carboxylic acid amides of general formula I
wherein all the groups are as hereinbefore defined, may be carried out by conventional methods of amide formation.
The coupling is preferably carried out using methods known from peptide chemistry (cf. e.g.
Houben-Weyl, Methoden der Organischen Chemie, Vol. 15/2), for example using carbodiimides such as e.g. dicyclohexylcarbodiimide (DCC), diisopropyl carbodiimide (DIC) or ethyl-(3-dimethylaminopropyI)-carbodiimide, 0-(1H-benzotriazol-1-y1)- N,N-N',N'-tetramethyluronium hexafluorophosphate (HBTU) or tetrafluoroborate (TBTU) or 1H-benzotriazol-1-yl-oxy-tris-(dimethylamino)-phosphonium hexafluorophosphate (BOP). By adding 1-hydroxybenzotriazole (HOBt) or 3-hydroxy-4-oxo-3,4-dihydro-1,2,3-benzotriazine (H0Obt) the reaction speed can be increased. The couplings are normally carried out with equimolar amounts of the coupling components as well as the coupling reagent in solvents such as dichloromethane, tetrahydrofuran (THF), acetonitrile, dimethyl formamide (DMF), dimethyl acetamide (DMA), N-methylpyrrolidone (NMP) or mixtures. If necessary, an auxiliary base such as diisopropylethylamine (DIPEA, HOnig base) is additionally used.
B) Amide coupling:
x R9 R7 el R3 R4 R2x/(( ) (R6)õ R2 R11 (IV) (V) An alternative method of preparing compounds of general formula I consists in linking carboxylic acids of general formula V, wherein all the groups are as hereinbefore defined, with amines of general formula IV, wherein all the groups are as hereinbefore defined.
The compounds of general formula V are either commercially obtainable or may be prepared by methods known from the literature It is also possible to convert the carboxylic acids of general formula V into carboxylic acid chlorides and then react these with amines of general formula IV. Carboxylic acid chlorides are synthesised by methods known from the literature (cf. e.g. Houben-Weyl, Methoden der Organischen Chemie, vol. E5/1).
-9..
(C) Reduction of the nitrile group:
Re Re Ra R7 R9 411 HN X., R7 R9 1 I I
N R"
N R"
(R6)n R2 R11 (R6) 2n R R11 (VI) (III) The reduction of a nitrile of general formula Vito an amine of general formula III, wherein the group R2 at the amine nitrogen denotes hydrogen and all the other groups are as hereinbefore defined, may be carried out under standard conditions of catalytic hydrogenolysis with a catalyst such as Raney nickel, for example, in a solvent such as ammoniacal methanol or ethanol or with a reducing agent such as lithium aluminium hydride or sodium borohydride in a solvent such as tetrahydrofuran, optionally in the presence of a io Lewis acid such as aluminium chloride.
Compounds of general formula III, wherein the group R2 at the amine nitrogen denotes not hydrogen but an alkyl group, for example, may also be prepared from compounds of general formula VI. Thus, for example, the reaction of a nitrile of general formula VI
with an alkyl Grignard reagent produces ketones which can be converted by reductive amination into the compounds of general formula III. The reductive amination is carried out using known methods, for example with a reducing agent such as sodium triacetoxyborohydride, sodium borohydride or sodium cyanoborohydride, conveniently in a solvent such as tetrahydrofuran or dichloromethane optionally substituted by the addition of acetic acid.
Alternatively the ketones obtained may also be converted into oximes. The subsequent reduction of the oximes then yields compounds of general formula III.
(D) nucleophilic aromatic substitution or transition-metal-catalysed coupling:
F F F
the enantiomers, the diastereomers, the mixtures and the salts thereof, particularly the physiologically acceptable salts thereof with organic or inorganic acids or bases.
In another aspect of the invention, a pharmaceutical formulation comprising a compound as described herein and one or more inert carriers and/or diluents is provided.
TERMS AND DEFINITIONS USED
Unless otherwise stated, all the substituents are independent of one another.
If for example io there are a plurality of C1_4-alkyl groups as substituents in one group, in the case of three substituents C1_4-alkyl, one may represent methyl, one n-propyl and one tert-butyl.
Within the scope of this application, in the definition of possible substituents, these may also be represented in the form of a structural formula. If present, an asterisk (*) in the structural formula of the substituent is to be understood as being the linking point to the rest of the molecule.
Also included in the subject matter of this invention are the compounds according to the invention, including the salts thereof, in which one or more hydrogen atoms, for example one, two, three, four or five hydrogen atoms, are replaced by deuterium.
By the term "C1_4-alkyl" (including those that are part of other groups) are meant alkyl groups with 1 to 4 carbon atoms. Examples include: methyl, ethyl, n-propyl, iso-propyl, n-butyl, iso-butyl, sec-butyl and tert-butyl. The abbreviations Me, Et, n-Pr, i-Pr, n-Bu, i-Bu, t-Bu, etc. may optionally also be used for the above-mentioned groups. Unless stated otherwise, the definitions propyl and butyl include all the possible isomeric forms of the groups in question.
Thus, for example, propyl includes n-propyl and iso-propyl, butyl includes iso-butyl, sec-butyl and tert-butyl.
Moreover the definitions mentioned previously also include those groups wherein each methylene group may be substituted by up to two and each methyl group may be substituted by up to three fluorine atoms.
By the term "Cm-cycloalkyl" (including those that are part of other groups) are meant cyclic alkyl groups with 3 to 6 carbon atoms. Examples include: cyclopropyl, cyclobutyl, cyclopentyl or cyclohexyl.
If they contain suitable basic functions, for example amino groups, compounds of general formula I may be converted, particularly for pharmaceutical use, into the physiologically acceptable salts thereof with inorganic or organic acids. Examples of inorganic acids for this purpose include hydrobromic acid, phosphoric acid, nitric acid, hydrochloric acid, sulphuric acid, methanesulphonic acid, ethanesulphonic acid, benzenesulphonic acid or p-toluenesulphonic acid, while organic acids that may be used include malic acid, succinic acid, acetic acid, fumaric acid, maleic acid, mandelic acid, lactic acid, tartaric acid or citric acid.
In addition, the compounds of general formula I, if they contain suitable carboxylic acid functions, may be converted into the physiologically acceptable salts thereof with inorganic or organic bases, particularly for pharmaceutical applications. Examples of inorganic bases include alkali or alkaline earth metal hydroxides, e.g. sodium hydroxide or potassium hydroxide, or carbonates, ammonia, zinc or ammonium hydroxides; examples of organic amines include diethylamine, triethylamine, ethanolamine, diethanolamine, triethanolamine, cyclohexylamine or dicyclohexylamine.
The compounds according to the invention may be present as racemates, provided that they have only one chiral element, but may also be obtained as pure enantiomers, i.e. In the (R) Or (S) form.
However, the application also includes the individual diastereomeric pairs of antipodes or mixtures thereof, which are obtained if there is more than one chiral element in the compounds of general formula I, as well as the individual optically active enantiomers of which the above-mentioned racemates are made up.
Compounds with a carbon double bond may be present in both the E and Z form.
=
If a compound is present in different tautomeric forms, the compound prepared is not limited to one tautomeric form but includes all the tautomeric forms. This also applies particularly to nitrogen-containing heteroaryls:
-N OH _____________________________________ N-N 0 PREPARATION METHODS
According to the invention the compounds of general formula I are obtained by methods io known per se, for example by the following methods:
(A) amide coupling:
RIO
( I )0 R3 R4 (R6)õ Ir R11 (II) (III) The linking of carboxylic acids of general formula II as shown, wherein all the groups are as hereinbefore defined, with amines of general formula III, wherein all the groups are as hereinbefore defined, to form carboxylic acid amides of general formula I
wherein all the groups are as hereinbefore defined, may be carried out by conventional methods of amide formation.
The coupling is preferably carried out using methods known from peptide chemistry (cf. e.g.
Houben-Weyl, Methoden der Organischen Chemie, Vol. 15/2), for example using carbodiimides such as e.g. dicyclohexylcarbodiimide (DCC), diisopropyl carbodiimide (DIC) or ethyl-(3-dimethylaminopropyI)-carbodiimide, 0-(1H-benzotriazol-1-y1)- N,N-N',N'-tetramethyluronium hexafluorophosphate (HBTU) or tetrafluoroborate (TBTU) or 1H-benzotriazol-1-yl-oxy-tris-(dimethylamino)-phosphonium hexafluorophosphate (BOP). By adding 1-hydroxybenzotriazole (HOBt) or 3-hydroxy-4-oxo-3,4-dihydro-1,2,3-benzotriazine (H0Obt) the reaction speed can be increased. The couplings are normally carried out with equimolar amounts of the coupling components as well as the coupling reagent in solvents such as dichloromethane, tetrahydrofuran (THF), acetonitrile, dimethyl formamide (DMF), dimethyl acetamide (DMA), N-methylpyrrolidone (NMP) or mixtures. If necessary, an auxiliary base such as diisopropylethylamine (DIPEA, HOnig base) is additionally used.
B) Amide coupling:
x R9 R7 el R3 R4 R2x/(( ) (R6)õ R2 R11 (IV) (V) An alternative method of preparing compounds of general formula I consists in linking carboxylic acids of general formula V, wherein all the groups are as hereinbefore defined, with amines of general formula IV, wherein all the groups are as hereinbefore defined.
The compounds of general formula V are either commercially obtainable or may be prepared by methods known from the literature It is also possible to convert the carboxylic acids of general formula V into carboxylic acid chlorides and then react these with amines of general formula IV. Carboxylic acid chlorides are synthesised by methods known from the literature (cf. e.g. Houben-Weyl, Methoden der Organischen Chemie, vol. E5/1).
-9..
(C) Reduction of the nitrile group:
Re Re Ra R7 R9 411 HN X., R7 R9 1 I I
N R"
N R"
(R6)n R2 R11 (R6) 2n R R11 (VI) (III) The reduction of a nitrile of general formula Vito an amine of general formula III, wherein the group R2 at the amine nitrogen denotes hydrogen and all the other groups are as hereinbefore defined, may be carried out under standard conditions of catalytic hydrogenolysis with a catalyst such as Raney nickel, for example, in a solvent such as ammoniacal methanol or ethanol or with a reducing agent such as lithium aluminium hydride or sodium borohydride in a solvent such as tetrahydrofuran, optionally in the presence of a io Lewis acid such as aluminium chloride.
Compounds of general formula III, wherein the group R2 at the amine nitrogen denotes not hydrogen but an alkyl group, for example, may also be prepared from compounds of general formula VI. Thus, for example, the reaction of a nitrile of general formula VI
with an alkyl Grignard reagent produces ketones which can be converted by reductive amination into the compounds of general formula III. The reductive amination is carried out using known methods, for example with a reducing agent such as sodium triacetoxyborohydride, sodium borohydride or sodium cyanoborohydride, conveniently in a solvent such as tetrahydrofuran or dichloromethane optionally substituted by the addition of acetic acid.
Alternatively the ketones obtained may also be converted into oximes. The subsequent reduction of the oximes then yields compounds of general formula III.
(D) nucleophilic aromatic substitution or transition-metal-catalysed coupling:
- 10 -N
X
Hal HN o N R"
(R6), I 2 R
R 11 (R6) Rõ I 2 11 R
(VII) (VIII) (VI) The reaction of an aniline of general formula VIII, wherein all the groups are as hereinbefore defined, with a nitrile of general formula VII, wherein X, R6 and n are as hereinbefore defined, and Hal denotes a fluorine, chlorine or bromine atom, is carried out using known methods, for example in a solvent such as tetrahydrofuran, dimethylformamide or dimethylsulphoxide and conveniently in the presence of a base such as triethylamine, sodium hydroxide solution or potassium carbonate at a temperature of 20 C to 160 C. If the aniline of general formula VIII is liquid, the reaction may also be carried out without a solvent io and additional base.
An alternative method of preparing compounds of general formula VI is the palladium-catalysed reaction of a nitrile of general formula VII, wherein Hal denotes bromine or chlorine, with an aniline of general formula VIII. Reaction conditions for this reaction, which is also known as a Buchwald-Hartwig reaction, are known from the literature.
Description of the method of binding the cynoBK1-receptor CHO cells that express the cynomolgus SKI-receptor are cultivated in "HAM'S F-Medium÷. The medium is removed from confluent cultures, the cells are washed with PBS
buffer, scraped off or detached using Versene and isolated by centrifuging.
Then the cells are homogenised in suspension, the homogenate is centrifuged and resuspended.
After the protein content has been determined 200 pl of the homogenate (50 to 250 pg protein/assay) are incubated for 60-180 minutes at ambient temperature with 0.5 to 5.0 nM
kallidine (DesArg10,Leu9), [3,4-ProlyI-3,43H(N)] and increasing concentrations of the test substance in a total volume of 250 pl. The incubation is stopped by rapid filtration through GF/B glass fibre filters that have been pre-treated with polyethyleneimine (0.3%). The radioactivity bound to the protein is measured with a TopCount NXT. The radioactivity bound in the
X
Hal HN o N R"
(R6), I 2 R
R 11 (R6) Rõ I 2 11 R
(VII) (VIII) (VI) The reaction of an aniline of general formula VIII, wherein all the groups are as hereinbefore defined, with a nitrile of general formula VII, wherein X, R6 and n are as hereinbefore defined, and Hal denotes a fluorine, chlorine or bromine atom, is carried out using known methods, for example in a solvent such as tetrahydrofuran, dimethylformamide or dimethylsulphoxide and conveniently in the presence of a base such as triethylamine, sodium hydroxide solution or potassium carbonate at a temperature of 20 C to 160 C. If the aniline of general formula VIII is liquid, the reaction may also be carried out without a solvent io and additional base.
An alternative method of preparing compounds of general formula VI is the palladium-catalysed reaction of a nitrile of general formula VII, wherein Hal denotes bromine or chlorine, with an aniline of general formula VIII. Reaction conditions for this reaction, which is also known as a Buchwald-Hartwig reaction, are known from the literature.
Description of the method of binding the cynoBK1-receptor CHO cells that express the cynomolgus SKI-receptor are cultivated in "HAM'S F-Medium÷. The medium is removed from confluent cultures, the cells are washed with PBS
buffer, scraped off or detached using Versene and isolated by centrifuging.
Then the cells are homogenised in suspension, the homogenate is centrifuged and resuspended.
After the protein content has been determined 200 pl of the homogenate (50 to 250 pg protein/assay) are incubated for 60-180 minutes at ambient temperature with 0.5 to 5.0 nM
kallidine (DesArg10,Leu9), [3,4-ProlyI-3,43H(N)] and increasing concentrations of the test substance in a total volume of 250 pl. The incubation is stopped by rapid filtration through GF/B glass fibre filters that have been pre-treated with polyethyleneimine (0.3%). The radioactivity bound to the protein is measured with a TopCount NXT. The radioactivity bound in the
- 11 -presence of 1.0 pM kallidine (DesArg10) is defined as non-specific binding.
The concentration binding curve may be analysed using computer-aided non-linear curve fitting to determine the corresponding K1 value for the test substance.
Test results of the cynoBK1-receptor binding assay:
Ki Example No.
[nM]
(1) 3.5 (2) 5.1 (3) 12 (4) 27 (5) 11 (6) 3.2 (7) 30 (8) 37 (9) 6.6 An essential structural feature of the new compounds is the 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid amide group which is contained in all the compounds according to the io invention and is in tautomeric equilibrium with the 6-hydroxy-pyridazine-4-carboxylic acid amide group:
HN H N H
Compared with the compounds from the prior art the new substances are characterised in that they exhibit a very strong 61-receptor blocking activity and at the same time have much better metabolic stability. The metabolic stability can be measured by determining the decomposition in human hepatocytes and using the rate of decomposition to calculate the clearance, which in turn is expressed as a percentage of the human hepatic blood flow (%Qh). A substance with a high metabolic clearance (e.g. >70%Qh) will presumably exhibit
The concentration binding curve may be analysed using computer-aided non-linear curve fitting to determine the corresponding K1 value for the test substance.
Test results of the cynoBK1-receptor binding assay:
Ki Example No.
[nM]
(1) 3.5 (2) 5.1 (3) 12 (4) 27 (5) 11 (6) 3.2 (7) 30 (8) 37 (9) 6.6 An essential structural feature of the new compounds is the 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid amide group which is contained in all the compounds according to the io invention and is in tautomeric equilibrium with the 6-hydroxy-pyridazine-4-carboxylic acid amide group:
HN H N H
Compared with the compounds from the prior art the new substances are characterised in that they exhibit a very strong 61-receptor blocking activity and at the same time have much better metabolic stability. The metabolic stability can be measured by determining the decomposition in human hepatocytes and using the rate of decomposition to calculate the clearance, which in turn is expressed as a percentage of the human hepatic blood flow (%Qh). A substance with a high metabolic clearance (e.g. >70%Qh) will presumably exhibit
- 12 -a shorter duration of activity in the human body than a substance that is metabolically more stable and thus has a lower clearance (e.g. <30%Qh). Thus, in the interests of achieving a long duration of activity it is highly advantageous if the active substance has a high metabolic stability (low clearance). Surprisingly the new substances exhibit a low clearance in human hepatocytes, as is apparent from the following Table:
Ki Clearance Example No.
[nM] [%Qh]
(1) 3.5 1 (3) 12 7 (5) 11 22 (6) 3.2 6 Description of method for determining metabolic clearance in human hepatocytes io The metabolic breakdown of the test substance is determined in a hepatocyte suspension.
Cryopreserved primary human hepatocytes are incubated in a suitable incubation medium (e.g. Dulbecco's modified eagle medium, DMEM) which contains 5% human serum.
After 30 minutes' pre-incubation in the incubator (37 C, 10% carbon dioxide) 5 pL of the test compound (80 pM, prepared from a 2 mM stock solution in dimethylsulphoxide and diluted 1:25 with incubation medium) are added to 395 pL of hepatocyte suspension (cell density in the range from 0.25 - 1 million cells/mL, typically 1 million cells/mL; final concentration of the test compound 1pM). The cells are incubated for 6 hours in an incubator with an orbital agitator. At times 0, 0.5, 1, 2, 4 and 6 h, 25 pL of the medium are removed in each case.
The medium removed is mixed with an excess of acetonitrile and centrifuged for 5 minutes.
The supernatant is removed, evaporated to dryness under nitrogen and taken up in a mixture of 25% methanol and 0.1% formic acid. The reduction in the concentration of the test substance in the incubation medium is determined by coupling liquid chromatography with electrospray mass spectrometry. The linear phase of the decrease in the concentration of the test substance in the medium is used for the calculation. The intrinsic clearance is calculated as follows: CL_INTRINSIC = dose / AUC = (CO/CD) / (AUD + clast/k) x 1000/60.
CO: initial concentration in the incubation [pin CD: cell density of the vital cells [10e6cells/mL], AUD:
Ki Clearance Example No.
[nM] [%Qh]
(1) 3.5 1 (3) 12 7 (5) 11 22 (6) 3.2 6 Description of method for determining metabolic clearance in human hepatocytes io The metabolic breakdown of the test substance is determined in a hepatocyte suspension.
Cryopreserved primary human hepatocytes are incubated in a suitable incubation medium (e.g. Dulbecco's modified eagle medium, DMEM) which contains 5% human serum.
After 30 minutes' pre-incubation in the incubator (37 C, 10% carbon dioxide) 5 pL of the test compound (80 pM, prepared from a 2 mM stock solution in dimethylsulphoxide and diluted 1:25 with incubation medium) are added to 395 pL of hepatocyte suspension (cell density in the range from 0.25 - 1 million cells/mL, typically 1 million cells/mL; final concentration of the test compound 1pM). The cells are incubated for 6 hours in an incubator with an orbital agitator. At times 0, 0.5, 1, 2, 4 and 6 h, 25 pL of the medium are removed in each case.
The medium removed is mixed with an excess of acetonitrile and centrifuged for 5 minutes.
The supernatant is removed, evaporated to dryness under nitrogen and taken up in a mixture of 25% methanol and 0.1% formic acid. The reduction in the concentration of the test substance in the incubation medium is determined by coupling liquid chromatography with electrospray mass spectrometry. The linear phase of the decrease in the concentration of the test substance in the medium is used for the calculation. The intrinsic clearance is calculated as follows: CL_INTRINSIC = dose / AUC = (CO/CD) / (AUD + clast/k) x 1000/60.
CO: initial concentration in the incubation [pin CD: cell density of the vital cells [10e6cells/mL], AUD:
- 13 -area under the curve [pM x h], clast: concentration of the last data point [pM], k: increase in the regression lines for the reduction in the test substance [h-1]. The intrinsic in vitro clearance calculated is now converted into the intrinsic in vivo clearance:
CL_INTRINSIC INVIVO [ml/min/kg] = (CL_INTRINSIC [pL/min/10e6cells] x hepatocellularity [10e6 cells/g liver] x liver factor [g/kg body weight]) / 1000 and the estimated human clearance is calculated using the well-stirred model:
CL [ml/min/kg] = CL_INTRINSIC_INVIVO [ml/min/kg] x hepatic blood flow [ml/mm/kg] /
(CL_INTRINSIC_INVIVO [ml/min/kg] + hepatic blood flow [ml/min/kg]).
The following parameters are used for the calculation: hepatocellularity, human: 120x10e6 cells / g liver; liver factor, human: 25.7 g liver / kg body weight; hepatic blood flow, human:
21 ml/(min x kg).
EXPERIMENTAL SECTION
Generally, there are mass spectra and 1H NMR spectra for the compounds that have been prepared. The ratios given for the eluants are in volume units of the solvents in question.
For ammonia, the given volume units are based on a concentrated solution of ammonia in water.
Unless indicated otherwise, the acid, base and salt solutions used for working up the reaction solutions are aqueous systems having the stated concentrations.
For chromatographic purification, silica gel from Millipore (MATREXTu, 35 to 70 pm) or Alox (E. Merck, Darmstadt, Alumina 90 standardized, 63 to 200 pm, article No.
1.01097.9050) is used.
In the descriptions of the experiments, the following abbreviations are used;
TLC thin layer chromatograph DMSO dimethylsulphoxide RP reverse phase
CL_INTRINSIC INVIVO [ml/min/kg] = (CL_INTRINSIC [pL/min/10e6cells] x hepatocellularity [10e6 cells/g liver] x liver factor [g/kg body weight]) / 1000 and the estimated human clearance is calculated using the well-stirred model:
CL [ml/min/kg] = CL_INTRINSIC_INVIVO [ml/min/kg] x hepatic blood flow [ml/mm/kg] /
(CL_INTRINSIC_INVIVO [ml/min/kg] + hepatic blood flow [ml/min/kg]).
The following parameters are used for the calculation: hepatocellularity, human: 120x10e6 cells / g liver; liver factor, human: 25.7 g liver / kg body weight; hepatic blood flow, human:
21 ml/(min x kg).
EXPERIMENTAL SECTION
Generally, there are mass spectra and 1H NMR spectra for the compounds that have been prepared. The ratios given for the eluants are in volume units of the solvents in question.
For ammonia, the given volume units are based on a concentrated solution of ammonia in water.
Unless indicated otherwise, the acid, base and salt solutions used for working up the reaction solutions are aqueous systems having the stated concentrations.
For chromatographic purification, silica gel from Millipore (MATREXTu, 35 to 70 pm) or Alox (E. Merck, Darmstadt, Alumina 90 standardized, 63 to 200 pm, article No.
1.01097.9050) is used.
In the descriptions of the experiments, the following abbreviations are used;
TLC thin layer chromatograph DMSO dimethylsulphoxide RP reverse phase
- 14 -Rt retention time tert tertiary TBTU 2-(1H-benzotriazol-1-y1)-1,1,3,3-tetramethyluronium-tetrafluoroborate THF tetrahydrofuran The following analytical HPLC methods were used:
Method 1: Column: Interchim Strategy C18, 5 pM, 4.6 x 50 mm Detection: 220 - 320 nnn Eluant A: water/ 0.1% acetic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 3.0 0.3 95.0 5.0 3.0 2.0 2.0 98.0 3.0 2.4 2.0 98.0 3.0 2.45 95.0 5.0 3.0 2.8 95.0 5.0 3.0 Method 2: Column: Merck Cromolith Flash RP18e, 4.6 x 25 mm Eluant A: water / 0.1% formic acid Eluant B: acetonitrile / 0.1% formic acid Gradient:
time in min %A %B flow rate in mL/min 0.0 90.0 10.0 1.6 2.7 10.0 90.0 1.6 3.0 10.0 90.0 1.6 3.3 90.0 10.0 1.6
Method 1: Column: Interchim Strategy C18, 5 pM, 4.6 x 50 mm Detection: 220 - 320 nnn Eluant A: water/ 0.1% acetic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 3.0 0.3 95.0 5.0 3.0 2.0 2.0 98.0 3.0 2.4 2.0 98.0 3.0 2.45 95.0 5.0 3.0 2.8 95.0 5.0 3.0 Method 2: Column: Merck Cromolith Flash RP18e, 4.6 x 25 mm Eluant A: water / 0.1% formic acid Eluant B: acetonitrile / 0.1% formic acid Gradient:
time in min %A %B flow rate in mL/min 0.0 90.0 10.0 1.6 2.7 10.0 90.0 1.6 3.0 10.0 90.0 1.6 3.3 90.0 10.0 1.6
- 15 -Method 3: Column: YMC-Pack ODS-AQ, 3 pM, 4.6 x 75 mm Eluant A: water! 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 2.0 10.0 90.0 1.6 5.0 10.0 90.0 1.6 5.5 90.0 10.0 1.6 Method 4: Column: Zorbax Stable Bond C18, 1.8 pM, 3 x 30 mm Eluant A: water /0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 1.0 10.0 90.0 1.6 2.5 10.0 90.0 1.6 2.75 95.0 5.0 1.6
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 2.0 10.0 90.0 1.6 5.0 10.0 90.0 1.6 5.5 90.0 10.0 1.6 Method 4: Column: Zorbax Stable Bond C18, 1.8 pM, 3 x 30 mm Eluant A: water /0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 1.0 10.0 90.0 1.6 2.5 10.0 90.0 1.6 2.75 95.0 5.0 1.6
- 16 -Method 5: Column: Sunfire 018, 3.5 pM, 4.6 x 50 mm Detection: 180 - 820 nm Eluant A: water / 0.1% trifluoroacetic acid Eluant B: acetonitrile / 0.1% trifluoroacetic acid Temperature: 40 C
Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.5 2.0 0.0 100.0 1.5 2.5 0.0 100.0 1.5 2.6 95.0 5.0 1.5 Method 6: Column: Sunfire C18, 3.5 pM, 4.6 x 50 mm Detection: 180 - 820 rim Eluant A: water /0.1% trifluoroacetic acid Eluant B: acetonitrile /0.1% trifluoroacetic acid Temperature: 40 C
Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.5 2.0 0.0 100.0 1.5 3.0 0.0 100.0 1.5 3.4 95.0 5.0 1.5 Method 7: Column: YMC-Pack ODS-AQ, 3 pM, 4.6 x 75 mm Eluant A: water / 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 4.5 10.0 90.0 1.6
Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.5 2.0 0.0 100.0 1.5 2.5 0.0 100.0 1.5 2.6 95.0 5.0 1.5 Method 6: Column: Sunfire C18, 3.5 pM, 4.6 x 50 mm Detection: 180 - 820 rim Eluant A: water /0.1% trifluoroacetic acid Eluant B: acetonitrile /0.1% trifluoroacetic acid Temperature: 40 C
Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.5 2.0 0.0 100.0 1.5 3.0 0.0 100.0 1.5 3.4 95.0 5.0 1.5 Method 7: Column: YMC-Pack ODS-AQ, 3 pM, 4.6 x 75 mm Eluant A: water / 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 4.5 10.0 90.0 1.6
- 17 -time in min %A %B flow rate in mL/min 5.0 10.0 90.0 1.6 5.50 90.0 10.0 1.6 Method 8: Column: Zorbax Stable Bond C18, 1.8 pM, 3 x 30 mm Eluant A: water! 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 2.00 50.0 50.0 1.6 2.25 10.0 90.0 1.6 2.50 10.0 90.0 1.6 2.75 95.0 5.0 1.6 Method 9: Column: Zorbax Stable Bond C18, 1.8 pM, 3 x 30 mm Eluant A: water! 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 2.25 10.0 90_0 1.6 2.50 10.0 90.0 1.6 2.75 95.0 5.0 1.6 Method 10: Column: Zorbax Stable Bond C18, 3.5 pM, 4.6 x 75 mm Eluant A: water / 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 2.00 50.0 50.0 1.6 2.25 10.0 90.0 1.6 2.50 10.0 90.0 1.6 2.75 95.0 5.0 1.6 Method 9: Column: Zorbax Stable Bond C18, 1.8 pM, 3 x 30 mm Eluant A: water! 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 2.25 10.0 90_0 1.6 2.50 10.0 90.0 1.6 2.75 95.0 5.0 1.6 Method 10: Column: Zorbax Stable Bond C18, 3.5 pM, 4.6 x 75 mm Eluant A: water / 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6
- 18 -time in min %A %B flow rate in mL/min 4.5 10.0 90.0 1.6 5.0 10.0 90.0 1.6 5.50 90.0 10.0 1.6 Method 11: Column: X Terra C18, 3.5 pM, 4.6 x 50 mm Detection: 180 - 820 nm Eluant A: water / 0.1% trifluoroacetic acid Eluant B: acetonitrile / 0.1% trifluoroacetic acid Temperature: 40 C
Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.5 2.0 0.0 100.0 1.5 3.0 0.0 100.0 1.5 3.4 95.0 5.0 1.5 Method 12: Column: Merck Cromolith Flash RP18e, 4.6 x 25 mm Eluant A: water / 0.1% formic acid Eluant B: acetonitrile /0.1% formic acid Gradient:
time in min %A %B flow rate in mL/min 0.0 90.0 10.0 1.6 2.7 10.0 90.0 1.6 3.0 10.0 90.0 1.6 3.3 95.0 5.0 1.6 Method 13: Column: Merck Cromolith SpeedROD RP-18e, 4.6 x 50 mm Eluant A: water /0.1% formic acid Eluant B: acetonitrile /0.1% formic acid Gradient:
Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.5 2.0 0.0 100.0 1.5 3.0 0.0 100.0 1.5 3.4 95.0 5.0 1.5 Method 12: Column: Merck Cromolith Flash RP18e, 4.6 x 25 mm Eluant A: water / 0.1% formic acid Eluant B: acetonitrile /0.1% formic acid Gradient:
time in min %A %B flow rate in mL/min 0.0 90.0 10.0 1.6 2.7 10.0 90.0 1.6 3.0 10.0 90.0 1.6 3.3 95.0 5.0 1.6 Method 13: Column: Merck Cromolith SpeedROD RP-18e, 4.6 x 50 mm Eluant A: water /0.1% formic acid Eluant B: acetonitrile /0.1% formic acid Gradient:
- 19 -time in min %A %B flow rate in mL/min 0.0 90.0 10.0 1.5 4.5 10.0 90.0 1.5 5.0 10.0 90.0 1.5 5.5 95.0 5.0 1.5 Method 14: Column: Zorbax Stable Bond C18, 3.5 pM, 4.6 x 75 mm Eluant A: water! 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 2.0 10.0 90.0 1.6 5.0 10.0 90.0 1.6 5.5 90.0 10.0 1.6 The following preparative methods were used for the reversed-phase chromatography:
Method 1: Column: Atlantis C18, 5 pM, 100 x 30 mm Detection: 210 - 500 nm Eluant A: water / 0.1% trifluoroacetic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 5 0.5 95.0 5.0 50 8.0 5.0 95.0 50 9.0 5.0 95.0 50 9.5 95.0 5.0 50 10.0 95.0 5.0 50 10.1 95.0 5.0 5
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 1.6 2.0 10.0 90.0 1.6 5.0 10.0 90.0 1.6 5.5 90.0 10.0 1.6 The following preparative methods were used for the reversed-phase chromatography:
Method 1: Column: Atlantis C18, 5 pM, 100 x 30 mm Detection: 210 - 500 nm Eluant A: water / 0.1% trifluoroacetic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 5 0.5 95.0 5.0 50 8.0 5.0 95.0 50 9.0 5.0 95.0 50 9.5 95.0 5.0 50 10.0 95.0 5.0 50 10.1 95.0 5.0 5
- 20 -Method 2: Column: Varian Pursuit 5 pM, 50 x 200 mm Eluant A: water! 0.1% trifluoroacetic acid Eluant B: acetonitrile 10.1% trifluoroacetic acid Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 180 1.15 95.0 5.0 180 12.4 2.0 98.0 180 14.0 2.0 98.0 180 15.3 95.0 5.0 180 15.3 95.0 5.5 180 Method 3: Column: YMC-Pack ODS-AQ 5 pM, 30 x 100 mm Eluant A: water! 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 50 2.0 95.0 5.0 50 6.0 10.0 90.0 50 12.0 10.0 90.0 50 13 90.0 10.0 50
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 180 1.15 95.0 5.0 180 12.4 2.0 98.0 180 14.0 2.0 98.0 180 15.3 95.0 5.0 180 15.3 95.0 5.5 180 Method 3: Column: YMC-Pack ODS-AQ 5 pM, 30 x 100 mm Eluant A: water! 0.15% formic acid Eluant B: acetonitrile Gradient:
time in min %A %B flow rate in mL/min 0.0 95.0 5.0 50 2.0 95.0 5.0 50 6.0 10.0 90.0 50 12.0 10.0 90.0 50 13 90.0 10.0 50
- 21 -Preparation of the starting compounds:
The compounds of general formula I may be prepared from the following intermediates A, B
and C:
R7 el Rs oR R R2 (R6) 2, =
A
The compounds of general formula I may be prepared from the following intermediates A, B
and C:
R7 el Rs oR R R2 (R6) 2, =
A
- 22 -AAV1 + AAV2 _________ R
NH
3'7\--- 1'21 i AAV1 + AAV3 R3R4 R s N Rio (R6)õ 2 Rii R9 )( R7 ah R9 3R 4 I '--Thf 3R 4 I :I'll' *
R R R2 x_ oR R R
R10 Xr\J 4411 R1 (R6) R2 R11 (R6) 2 Rii R2 0 Rs R2 0R9 , I
R
HN
+I 00 o R3 R4 42 )>/--N
(R6)9 42 R11 (R6), R2 R11 A
AAV 1: amide coupling A solution of the carboxylic acid component (1 mol-equivalent), triethylamine (2.5 mol-equivalents) and TBTU (1.1 mol-equivalents) in THE was stirred for 30 minutes at ambient temperature. Then the amine component (1.1 mol-equivalent as hydrochloride) was added and stirring was continued overnight. Then the mixture was evaporated down, mixed with water, made alkaline with dilute potassium carbonate solution and extracted with ethyl acetate. The product was isolated and purified by column chromatography (either silica gel or io reversed phase chromatography).
AAV 2: Ester hydrolysis 2N sodium hydroxide solution (2 mol-equivalents) was added to a solution of the ester (1 mol-equivalent) in methanol and the mixture was stirred for 1 to 5 hours at ambient =
NH
3'7\--- 1'21 i AAV1 + AAV3 R3R4 R s N Rio (R6)õ 2 Rii R9 )( R7 ah R9 3R 4 I '--Thf 3R 4 I :I'll' *
R R R2 x_ oR R R
R10 Xr\J 4411 R1 (R6) R2 R11 (R6) 2 Rii R2 0 Rs R2 0R9 , I
R
HN
+I 00 o R3 R4 42 )>/--N
(R6)9 42 R11 (R6), R2 R11 A
AAV 1: amide coupling A solution of the carboxylic acid component (1 mol-equivalent), triethylamine (2.5 mol-equivalents) and TBTU (1.1 mol-equivalents) in THE was stirred for 30 minutes at ambient temperature. Then the amine component (1.1 mol-equivalent as hydrochloride) was added and stirring was continued overnight. Then the mixture was evaporated down, mixed with water, made alkaline with dilute potassium carbonate solution and extracted with ethyl acetate. The product was isolated and purified by column chromatography (either silica gel or io reversed phase chromatography).
AAV 2: Ester hydrolysis 2N sodium hydroxide solution (2 mol-equivalents) was added to a solution of the ester (1 mol-equivalent) in methanol and the mixture was stirred for 1 to 5 hours at ambient =
- 23 -temperature. Then it was acidified with acetic acid and the mixture was evaporated to dryness in vacuo. The crude product thus obtained was purified in the normal way by column chromatography on silica gel.
AAV 3: Cleaving the tert-butyloxycarbonyl protective group A solution of the tert-butoxycarbonyl-amino compound (1 mol-equivalent) in dichloromethane was combined with trifluoroacetic acid (3 to 10 mol-equivalents) and stirred at ambient temperature until the protective group had been cleaved completely. The reaction mixture was then evaporated to dryness and the crude product thus obtained was purified by is chromatography.
AAV 4:Preparation of the intermediate A
R
(R6)n R11 R7 ei R9R7 9 R
R
H,N , N " yõ/--I N = 0 Ri (R6)n H R11 nH R11 Alkyl RB Alkyl R9 R7 R9 HOX, R7 R9 avik N R10 R"
(R6)n H R11 (R6)n H R11 Alkyl R8 X, R7 40 (R6)n H R11
AAV 3: Cleaving the tert-butyloxycarbonyl protective group A solution of the tert-butoxycarbonyl-amino compound (1 mol-equivalent) in dichloromethane was combined with trifluoroacetic acid (3 to 10 mol-equivalents) and stirred at ambient temperature until the protective group had been cleaved completely. The reaction mixture was then evaporated to dryness and the crude product thus obtained was purified by is chromatography.
AAV 4:Preparation of the intermediate A
R
(R6)n R11 R7 ei R9R7 9 R
R
H,N , N " yõ/--I N = 0 Ri (R6)n H R11 nH R11 Alkyl RB Alkyl R9 R7 R9 HOX, R7 R9 avik N R10 R"
(R6)n H R11 (R6)n H R11 Alkyl R8 X, R7 40 (R6)n H R11
- 24 -A solution of the aniline component (1 mol-equivalent) and a strong base such as e.g.
potassium-tert-butoxide (1 mol-equivalent) in DMSO was stirred for one hour at ambient temperature, then combined with the 4-fluoro-benzonitrile component (1 mol-equivalent) and stirred overnight at approx. 80 C. For working up the mixture was filtered through Alox and evaporated to dryness in vacuo.
The nitrile group of the diphenylamine intermediate product thus obtained was then reduced to the aminomethyl group with the addition of Raney nickel at 55 C and 3 bar excess hydrogen pressure and the product obtained was purified by chromatography.
In order to prepare the intermediate A with an alpha-alkylbenzyl group (e.g.
Al, A4, A5) the nitrile derivative (1 mol-equivalent) was dissolved in diethyl ether and at 0 to 5 C it was added with stirring to a solution of alkylnnagnesium bromide (4 mol-equivalents) in diethyl ether and then stirred for another 30 minutes approx. The reaction mixture was then stirred into 1M hydrochloric acid at -5 C and the alkylketone thus obtained was isolated and purified by chromatography in the usual way.
A solution of the ketone thus obtained (1 mol-equivalent) in acetonitrile was combined with triethylamine (2 mol-equivalents) and hydroxylannine-hydrochloride (1.3 mol-equivalents) and refluxed for 4 hours. Then water was added and the mixture was extracted with dichloromethane. The resulting oxime was isolated from the organic phase and purified by conventional methods.
A solution of the oxime (1 mol-equivalent) in methanol was combined with methanolic hydrochloric acid (6.6 mol-equivalents). After the addition of zinc powder (1.4 mol-equivalents) the mixture was refluxed for 3 hours with stirring. After cooling the mixture was combined with water and extracted with dichloromethane. If necessary, the amine thus obtained was purified by chromatography.
Another possible way of reducing the oxime to the corresponding amine is by catalytic hydrogenation. For this, the oxime was hydrogenated in methanolic ammonia solution after the addition of Raney nickel at 50 C and at an excess hydrogen pressure of 50 psi until the uptake of hydrogen had ended. If necessary, the amine thus obtained was purified by chromatography.
potassium-tert-butoxide (1 mol-equivalent) in DMSO was stirred for one hour at ambient temperature, then combined with the 4-fluoro-benzonitrile component (1 mol-equivalent) and stirred overnight at approx. 80 C. For working up the mixture was filtered through Alox and evaporated to dryness in vacuo.
The nitrile group of the diphenylamine intermediate product thus obtained was then reduced to the aminomethyl group with the addition of Raney nickel at 55 C and 3 bar excess hydrogen pressure and the product obtained was purified by chromatography.
In order to prepare the intermediate A with an alpha-alkylbenzyl group (e.g.
Al, A4, A5) the nitrile derivative (1 mol-equivalent) was dissolved in diethyl ether and at 0 to 5 C it was added with stirring to a solution of alkylnnagnesium bromide (4 mol-equivalents) in diethyl ether and then stirred for another 30 minutes approx. The reaction mixture was then stirred into 1M hydrochloric acid at -5 C and the alkylketone thus obtained was isolated and purified by chromatography in the usual way.
A solution of the ketone thus obtained (1 mol-equivalent) in acetonitrile was combined with triethylamine (2 mol-equivalents) and hydroxylannine-hydrochloride (1.3 mol-equivalents) and refluxed for 4 hours. Then water was added and the mixture was extracted with dichloromethane. The resulting oxime was isolated from the organic phase and purified by conventional methods.
A solution of the oxime (1 mol-equivalent) in methanol was combined with methanolic hydrochloric acid (6.6 mol-equivalents). After the addition of zinc powder (1.4 mol-equivalents) the mixture was refluxed for 3 hours with stirring. After cooling the mixture was combined with water and extracted with dichloromethane. If necessary, the amine thus obtained was purified by chromatography.
Another possible way of reducing the oxime to the corresponding amine is by catalytic hydrogenation. For this, the oxime was hydrogenated in methanolic ammonia solution after the addition of Raney nickel at 50 C and at an excess hydrogen pressure of 50 psi until the uptake of hydrogen had ended. If necessary, the amine thus obtained was purified by chromatography.
- 25 -Preparation of the Intermediates A
HN R7 so R9 (R6)n R2 R11 The following intermediates Al to A31 were prepared according to general working method AAV4:
intermediate Al: (6-aminomethyl-pyridin-3-y1)-(4-chloro-2-trifluoromethyl-pheny1)-amine isH2N CI , F F
HPLC: R = 1.74 minutes (method 13) io Mass spectrum (ES1): [M+H]+ = 302 intermediate A2: (4-aminomethyl-phenyl)-(4-fluoro-2-trifluoromethyl-pheny1)-amine F F F
Mass spectrum (ES1): [M+H]+ = 285 thin layer chromatogram (silica gel, CH2C12/ethanol 19:1): 1=if = 0.16 intermediate A3: (6-aminomethyl-pyridin-3-y1)-(4-fluoro-2-trifluoromethyl-pheny1)-amine F F
HN R7 so R9 (R6)n R2 R11 The following intermediates Al to A31 were prepared according to general working method AAV4:
intermediate Al: (6-aminomethyl-pyridin-3-y1)-(4-chloro-2-trifluoromethyl-pheny1)-amine isH2N CI , F F
HPLC: R = 1.74 minutes (method 13) io Mass spectrum (ES1): [M+H]+ = 302 intermediate A2: (4-aminomethyl-phenyl)-(4-fluoro-2-trifluoromethyl-pheny1)-amine F F F
Mass spectrum (ES1): [M+H]+ = 285 thin layer chromatogram (silica gel, CH2C12/ethanol 19:1): 1=if = 0.16 intermediate A3: (6-aminomethyl-pyridin-3-y1)-(4-fluoro-2-trifluoromethyl-pheny1)-amine F F
- 26 -HPLC: Rt = 2.06 minutes (method 3) Mass spectrum (ES!): [M+H]+ = 286; [M-H]- = 284 intermediate A4: (4-aminomethy1-3-fluoro-pheny1)-(4-fluoro-2-trifluoromethyl-pheny1)-amine F I
FN
F F
Mass spectrum (ES!): [M+H]+ = 303 thin layer chromatogram (silica gel, CH2C12/ethanol 19:1): Rf = 0.08 intermediate A5: (4-aminomethy1-3-fluoro-pheny1)-(2-trifluoromethyl-pheny1)-amine H2N lel F F
Mass spectrum (ES1): EM-H]- = 283 thin layer chromatogram (silica gel, CH2C12/ethanol 19:1): Rf= 0.09 intermediate A6: (4-aminomethyl-phenyl)-(2-trifluoromethyl-phenyl)-amine F F
HPLC: Rt = 1.36 minutes (method 1) Mass spectrum (ES!): [M+H-NH3]+ = 250 intermediate A7: (4-aminomethyl-pheny1)-(4-chloro-2-trifluoromethyl-pheny1)-amine
FN
F F
Mass spectrum (ES!): [M+H]+ = 303 thin layer chromatogram (silica gel, CH2C12/ethanol 19:1): Rf = 0.08 intermediate A5: (4-aminomethy1-3-fluoro-pheny1)-(2-trifluoromethyl-pheny1)-amine H2N lel F F
Mass spectrum (ES1): EM-H]- = 283 thin layer chromatogram (silica gel, CH2C12/ethanol 19:1): Rf= 0.09 intermediate A6: (4-aminomethyl-phenyl)-(2-trifluoromethyl-phenyl)-amine F F
HPLC: Rt = 1.36 minutes (method 1) Mass spectrum (ES!): [M+H-NH3]+ = 250 intermediate A7: (4-aminomethyl-pheny1)-(4-chloro-2-trifluoromethyl-pheny1)-amine
- 27 -F F
Mass spectrum (ESI): [M+H-NH3]+ = 284/286 intermediate A8: (4-aminomethy1-3-fluoro-pheny1)-(4-chloro-2-trifluoromethyl-pheny1)-amine F F
HPLC: Rt= 1.83 minutes (method 2) intermediate A9: (4-aminomethyl-phenyl)-(4-bromo-2-trifluoromethyl-phenyl)-amine H2N Br el F F
HPLC: R = 1.81 minutes (method 2) Preparation of the Intermediates B
12 o OH
The following Intermediate B1 was prepared by amide coupling according to general working method AAV1 and subsequent ester saponification according to general working method AAV2:
intermediate B1: (S)-3-[(6-oxo-1,6-dihydro-pyridazine-4-carbony1)-amino]-tetrahydro-furan-3-carboxylic acid
Mass spectrum (ESI): [M+H-NH3]+ = 284/286 intermediate A8: (4-aminomethy1-3-fluoro-pheny1)-(4-chloro-2-trifluoromethyl-pheny1)-amine F F
HPLC: Rt= 1.83 minutes (method 2) intermediate A9: (4-aminomethyl-phenyl)-(4-bromo-2-trifluoromethyl-phenyl)-amine H2N Br el F F
HPLC: R = 1.81 minutes (method 2) Preparation of the Intermediates B
12 o OH
The following Intermediate B1 was prepared by amide coupling according to general working method AAV1 and subsequent ester saponification according to general working method AAV2:
intermediate B1: (S)-3-[(6-oxo-1,6-dihydro-pyridazine-4-carbony1)-amino]-tetrahydro-furan-3-carboxylic acid
- 28 -,N
HN
HPLC: R = 0.33 minutes (method 2) Mass spectrum (ESI): [M+H]+ = 254 The following Intermediate B2 may be prepared analogously:
intermediate B2: 1-[(6-oxo-1,6-dihydro-pyridazine-4-carbonyl)-amino]-cyclopropanecarboxylic acid ,N
HN
H
0 __ Preparation of the Intermediates C
1-11µ1X. R7 it& R9 "II RI
(R6), R2 R11 The following Intermediates Cl to C6 were prepared by amide coupling according to general working method AAV1 and subsequent cleaving of the tert-butyloxycarbonyl-protective group according to general working method AAV3:
intermediate Cl: 1-amino-cyclopropanecarboxylic acid45-(4-chloro-2-trifluoronnethyl-phenylamino)-pyridin-2-ylmethyI]-amide
HN
HPLC: R = 0.33 minutes (method 2) Mass spectrum (ESI): [M+H]+ = 254 The following Intermediate B2 may be prepared analogously:
intermediate B2: 1-[(6-oxo-1,6-dihydro-pyridazine-4-carbonyl)-amino]-cyclopropanecarboxylic acid ,N
HN
H
0 __ Preparation of the Intermediates C
1-11µ1X. R7 it& R9 "II RI
(R6), R2 R11 The following Intermediates Cl to C6 were prepared by amide coupling according to general working method AAV1 and subsequent cleaving of the tert-butyloxycarbonyl-protective group according to general working method AAV3:
intermediate Cl: 1-amino-cyclopropanecarboxylic acid45-(4-chloro-2-trifluoronnethyl-phenylamino)-pyridin-2-ylmethyI]-amide
- 29 -H2Nck, N Cl N
H
F F
F
HPLC: Rt = 1.55 minutes (method 13) Mass spectrum (ES1): [M-H]- = 383 intermediate C2: 1-amino-cyclopropanecarboxylic acid-[5-(4-fluoro-2-trifluoromethyl-phenylamino)-pyridin-2-ylmethy1]-amide o F
___________________________________ hi I
el ri F F
F
HPLC: Rt = 2.33 minutes (method 7) Mass spectrum (ES1): [M+H]+ = 369; [M-H]- = 367 lo intermediate C3: (S)-3-amino-tetrahydrofuran-3-carboxlic acid-2-fluoro-4-(4-fluoro-2-tri-fluoromethyl-phenylamino)-benzylamide o oi,, H2N õ, N
H SI el F
H
F F
F
Mass spectrum (ES1): [M+H]+ = 416 intermediate C4: (S)-3-amino-tetrahydrofuran-3-carboxylic acid-4-(4-fluoro-trifluoromethyl-phenylamino)-benzylamide
H
F F
F
HPLC: Rt = 1.55 minutes (method 13) Mass spectrum (ES1): [M-H]- = 383 intermediate C2: 1-amino-cyclopropanecarboxylic acid-[5-(4-fluoro-2-trifluoromethyl-phenylamino)-pyridin-2-ylmethy1]-amide o F
___________________________________ hi I
el ri F F
F
HPLC: Rt = 2.33 minutes (method 7) Mass spectrum (ES1): [M+H]+ = 369; [M-H]- = 367 lo intermediate C3: (S)-3-amino-tetrahydrofuran-3-carboxlic acid-2-fluoro-4-(4-fluoro-2-tri-fluoromethyl-phenylamino)-benzylamide o oi,, H2N õ, N
H SI el F
H
F F
F
Mass spectrum (ES1): [M+H]+ = 416 intermediate C4: (S)-3-amino-tetrahydrofuran-3-carboxylic acid-4-(4-fluoro-trifluoromethyl-phenylamino)-benzylamide
- 30 -F
F F
HPLC: Rt = 1.99 minutes (method 2) Mass spectrum (ESI): [M+H]+ = 398 intermediate C5: (S)-3-amino-tetrahydrofuran-3-carboxylic acid-4-(4-chloro-trifluoromethyl-phenylamino)-benzylamide F F
HPLC: Rt = 2.41 minutes (method 2) intermediate C6: (S)-3-amino-tetrahydrofuran-3-carboxylic acid 2-fluoro-4-(2-trifluoromethyl-phenylamino)-benzylamide H
F F
Mass spectrum (ESI): [M+H]+ = 398 Preparation of the End Compounds:
F F
HPLC: Rt = 1.99 minutes (method 2) Mass spectrum (ESI): [M+H]+ = 398 intermediate C5: (S)-3-amino-tetrahydrofuran-3-carboxylic acid-4-(4-chloro-trifluoromethyl-phenylamino)-benzylamide F F
HPLC: Rt = 2.41 minutes (method 2) intermediate C6: (S)-3-amino-tetrahydrofuran-3-carboxylic acid 2-fluoro-4-(2-trifluoromethyl-phenylamino)-benzylamide H
F F
Mass spectrum (ESI): [M+H]+ = 398 Preparation of the End Compounds:
- 31 -Example 1: 6-oxo-5,6-dihydro-pyridazine-4-carboxylic acid-(14[5-(4-fluoro-trifluoromethyl-phenylamino)-pyridin-2-ylmethy1]-carbamoy1}-cyclopropy1)-amide ,N
F
0 ________________________________ F FE
Prepared from intermediate C2 and 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid according to AAV1.
C22H15F4N603 (490.42) Rt = 2.80 minutes (method 7) Example 2: 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-(14[5-(4-chloro-2-trifluoromethyl-phenylamino)-pyridin-2-ylmethyll-carbannoy1}-cyclopropy1)-amide oxii Ai Cl H
0 ______________________________ F F
Prepared from intermediate Cl and 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid according tO AAV1.
C22H18C1F3N603 (506.87) Rt = 2.13 minutes (method 2) Example 3: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-(3-[4-(4-fluoro-2-trifluoromethyl-phenylamino)-benzylcarbamoyl]-tetrahydrofuran-3-yll-amide
F
0 ________________________________ F FE
Prepared from intermediate C2 and 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid according to AAV1.
C22H15F4N603 (490.42) Rt = 2.80 minutes (method 7) Example 2: 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-(14[5-(4-chloro-2-trifluoromethyl-phenylamino)-pyridin-2-ylmethyll-carbannoy1}-cyclopropy1)-amide oxii Ai Cl H
0 ______________________________ F F
Prepared from intermediate Cl and 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid according tO AAV1.
C22H18C1F3N603 (506.87) Rt = 2.13 minutes (method 2) Example 3: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-(3-[4-(4-fluoro-2-trifluoromethyl-phenylamino)-benzylcarbamoyl]-tetrahydrofuran-3-yll-amide
- 32 F F F
Prepared from intermediate C4 and 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid according to AAV1.
C24H21F4N504 (519.45) Rt = 2.39 minutes (method 2) Example 4: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-{344-(4-chloro-2-trifluoromethyl-phenylamino)-benzylcarbamoylHetrahydrofuran-3-yll-amide F F F
Prepared from intermediates A7 and B1 according to AAV1.
C24H21CIF3N504 (535.91) Rt = 2.28 minutes (method 2) Example 5: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-{314-(2-trifluoromethyl-phenylarnino)-benzylcarbarnoyI]-tetrahydrofuran-3-yll-amide Op F F
Prepared from intermediates A6 and B1 according to AAV1.
C24H22F3N504 (501.46)
Prepared from intermediate C4 and 6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid according to AAV1.
C24H21F4N504 (519.45) Rt = 2.39 minutes (method 2) Example 4: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-{344-(4-chloro-2-trifluoromethyl-phenylamino)-benzylcarbamoylHetrahydrofuran-3-yll-amide F F F
Prepared from intermediates A7 and B1 according to AAV1.
C24H21CIF3N504 (535.91) Rt = 2.28 minutes (method 2) Example 5: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-{314-(2-trifluoromethyl-phenylarnino)-benzylcarbarnoyI]-tetrahydrofuran-3-yll-amide Op F F
Prepared from intermediates A6 and B1 according to AAV1.
C24H22F3N504 (501.46)
- 33 -Rt = 2.09 minutes (method 2) Example 6: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-{342-fluoro-4-(4-fluoro-2-tri-fluoromethyl-phenylamino)-benzylcarbamoylHetrahydrofuran-3-yll-amide H el F F
Prepared from intermediates A4 and B1 according to AAV1.
C24.H20F5N504 (537.44) Rt = 2.15 minutes (method 2) Example 7: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-{344-(4-chloro-2-trifluoromethyl-phenylamino)-2-fluoro-benzylcarbamoylHetrahydrofuran-3-y1}-amide CI
0 Lci) F
F FE
Prepared from intermediates A8 and B1 according to AAV1.
C241-120C1F4N504 (553.90) Rt = 2.31 minutes (method 2) Example 8: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-{314-(4-bromo-2-trifluoromethyl-phenylamino)-benzylcarbamoylHetrahydrofuran-3-yll-amide
Prepared from intermediates A4 and B1 according to AAV1.
C24.H20F5N504 (537.44) Rt = 2.15 minutes (method 2) Example 7: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-{344-(4-chloro-2-trifluoromethyl-phenylamino)-2-fluoro-benzylcarbamoylHetrahydrofuran-3-y1}-amide CI
0 Lci) F
F FE
Prepared from intermediates A8 and B1 according to AAV1.
C241-120C1F4N504 (553.90) Rt = 2.31 minutes (method 2) Example 8: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid-{314-(4-bromo-2-trifluoromethyl-phenylamino)-benzylcarbamoylHetrahydrofuran-3-yll-amide
- 34 -N 00 Br F F
Prepared from intermediates A9 and B1 according to AAV1.
C24H BrF3N504 (580.35) Rt = 2.32 minutes (method 2) Example 9: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid {342-fluoro-4-(2-trifluoromethyl-phenylamino)-benzylcarbamoylpetrahydro-furan-3-y1}-amide 1\1, ON
110=
F F
Prepared from intermediates A5 and B1 according to AAV1.
C24H21F4N50.4 (519.45) Rt = 1.35 minutes (method 7) mass spectroscopy (ESI): [M+FI] = 520 = 518 The following Examples describe pharmaceutical formulations which contain as active substance any desired compound of general formula I, without restricting the scope of the present invention thereto:
Prepared from intermediates A9 and B1 according to AAV1.
C24H BrF3N504 (580.35) Rt = 2.32 minutes (method 2) Example 9: (S)-6-oxo-1,6-dihydro-pyridazine-4-carboxylic acid {342-fluoro-4-(2-trifluoromethyl-phenylamino)-benzylcarbamoylpetrahydro-furan-3-y1}-amide 1\1, ON
110=
F F
Prepared from intermediates A5 and B1 according to AAV1.
C24H21F4N50.4 (519.45) Rt = 1.35 minutes (method 7) mass spectroscopy (ESI): [M+FI] = 520 = 518 The following Examples describe pharmaceutical formulations which contain as active substance any desired compound of general formula I, without restricting the scope of the present invention thereto:
- 35 -Example I
Dry ampoule with 75 mq of active compound per 10 ml Composition:
Active compound 75.0 mg Mannitol 500 mg Water for injection ad 10.0 ml Production:
Active compound and mannitol are dissolved in water. The charged ampoules are freeze dried. Water for injection is used to dissolve to give the solution ready for use.
Example II
Tablet with 50 mg of active compound Composition:
(1) Active compound 50.0 mg (2) Lactose 98.0 mg (3) Maize starch 50.0 mg (4) Polyvinylpyrrolidone 15.0 mg (5) Magnesium stearate 2.0 mq 215.0 mg Production:
(1), (2) and (3) are mixed and granulated with an aqueous solution of (4). (5) is admixed to the dry granules. Tablets are compressed from this mixture, biplanar with a bevel on both sides and dividing groove on one side.
Diameter of the tablets: 9 mm.
Dry ampoule with 75 mq of active compound per 10 ml Composition:
Active compound 75.0 mg Mannitol 500 mg Water for injection ad 10.0 ml Production:
Active compound and mannitol are dissolved in water. The charged ampoules are freeze dried. Water for injection is used to dissolve to give the solution ready for use.
Example II
Tablet with 50 mg of active compound Composition:
(1) Active compound 50.0 mg (2) Lactose 98.0 mg (3) Maize starch 50.0 mg (4) Polyvinylpyrrolidone 15.0 mg (5) Magnesium stearate 2.0 mq 215.0 mg Production:
(1), (2) and (3) are mixed and granulated with an aqueous solution of (4). (5) is admixed to the dry granules. Tablets are compressed from this mixture, biplanar with a bevel on both sides and dividing groove on one side.
Diameter of the tablets: 9 mm.
- 36 -Example III
Tablet with 350 mq of active compound Composition:
(1) Active compound 350.0 mg (2) Lactose 136.0 mg (3) Maize starch 80.0 mg (4) Polyvinylpyrrolidone 30.0 mg (5) Magnesium stearate 4.0 mq 600.0 mg Production:
(1), (2) and (3) are mixed and granulated with an aqueous solution of (4). (5) is admixed to the dry granules. Tablets are compressed from this mixture, biplanar with a bevel on both sides and dividing groove on one side.
Diameter of the tablets: 12 mm.
Example IV
Capsule with 50 mq of active compound Composition:
(1) Active compound 50.0 mg (2) Maize starch dried 58.0 mg (3) Lactose powdered 50.0 mg (4) Magnesium stearate 2.0 mg 160.0 mg Production:
(1) is triturated with (3). This trituration is added to the mixture of (2) and (4) with vigorous mixing.
This powder mixture is packed into hard gelatine two-piece capsules of size 3 in a capsule-filling machine.
Tablet with 350 mq of active compound Composition:
(1) Active compound 350.0 mg (2) Lactose 136.0 mg (3) Maize starch 80.0 mg (4) Polyvinylpyrrolidone 30.0 mg (5) Magnesium stearate 4.0 mq 600.0 mg Production:
(1), (2) and (3) are mixed and granulated with an aqueous solution of (4). (5) is admixed to the dry granules. Tablets are compressed from this mixture, biplanar with a bevel on both sides and dividing groove on one side.
Diameter of the tablets: 12 mm.
Example IV
Capsule with 50 mq of active compound Composition:
(1) Active compound 50.0 mg (2) Maize starch dried 58.0 mg (3) Lactose powdered 50.0 mg (4) Magnesium stearate 2.0 mg 160.0 mg Production:
(1) is triturated with (3). This trituration is added to the mixture of (2) and (4) with vigorous mixing.
This powder mixture is packed into hard gelatine two-piece capsules of size 3 in a capsule-filling machine.
- 37 -Example V
Capsules with 350 mci of active compound Composition:
(1) Active compound 350.0 mg (2) Maize starch dried 46.0 mg (3) Lactose powdered 30.0 mg (4) Magnesium stearate 4.0 mg 430.0 mg Production:
(1) is triturated with (3). This trituration is added to the mixture of (2) and (4) with vigorous stirring.
This powder mixture is packed into hard gelatine two-piece capsules of size 0 in a capsule-filling machine.
Example VI
Suppositories with 100 mg of active compound 1 suppository comprises:
Active compound 100.0 mg Polyethylene glycol (M.W. 1500) 600.0 mg Polyethylene glycol (M.W. 6000) 460.0 mg Polyethylene sorbitan monostearate 840.0 mg 2000.0 mg
Capsules with 350 mci of active compound Composition:
(1) Active compound 350.0 mg (2) Maize starch dried 46.0 mg (3) Lactose powdered 30.0 mg (4) Magnesium stearate 4.0 mg 430.0 mg Production:
(1) is triturated with (3). This trituration is added to the mixture of (2) and (4) with vigorous stirring.
This powder mixture is packed into hard gelatine two-piece capsules of size 0 in a capsule-filling machine.
Example VI
Suppositories with 100 mg of active compound 1 suppository comprises:
Active compound 100.0 mg Polyethylene glycol (M.W. 1500) 600.0 mg Polyethylene glycol (M.W. 6000) 460.0 mg Polyethylene sorbitan monostearate 840.0 mg 2000.0 mg
Claims (13)
1. Compounds of general formula I
wherein R1 denotes the group R2 denotes H or CH3, R3 and R4 together with the carbon atom to which they are bound denote a C3-6-cycloalkylene group wherein a -CH2 unit may be replaced by an oxygen atom, R6 denotes H or CH3, R6 denotes H, F, CI or methyl, R7 denotes H, F, CI, Br, -CN, C1-4-alkyl, CF3, CHF2, R8 denotes H, R9 denotes F, CI, Br, C1-4-alkyl, -O-C1-4-alkyl, -S-C1-4-alkyl, R10 denotes H, R11 denotes F, CI, Br, -CN, C1-4-alkyl, CF3, CHF2, and X denotes CH or N, the enantiomers, the diastereomers, the mixtures and the salts thereof.
wherein R1 denotes the group R2 denotes H or CH3, R3 and R4 together with the carbon atom to which they are bound denote a C3-6-cycloalkylene group wherein a -CH2 unit may be replaced by an oxygen atom, R6 denotes H or CH3, R6 denotes H, F, CI or methyl, R7 denotes H, F, CI, Br, -CN, C1-4-alkyl, CF3, CHF2, R8 denotes H, R9 denotes F, CI, Br, C1-4-alkyl, -O-C1-4-alkyl, -S-C1-4-alkyl, R10 denotes H, R11 denotes F, CI, Br, -CN, C1-4-alkyl, CF3, CHF2, and X denotes CH or N, the enantiomers, the diastereomers, the mixtures and the salts thereof.
2. Compounds of general formula I according to claim 1, wherein R1, R3, R4, R5, R6, R7, R8, R9, R10, R11, n and X are defined as in claim 1 and R2 denotes H, the enantiomers, the diastereomers, the mixtures and the salts thereof.
3. The following compound of general formula I according to claim 1:
4. The compound:
5. The compound:
6. The compound:
7. The compound:
8. The compound:
9. The compound:
10. The compound:
11. Physiologically acceptable salt of a compound as defined in claim 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 with an inorganic or organic acid or base.
12. Pharmaceutical formulation comprising a compound as defined in claim 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 or a physiologically acceptable salt according to claim 11 together with one or more inert carriers and/or diluents.
13. Use of a compound as defined in claim 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 as a bradykinin B1 antagonist.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/EP2010/052232 WO2010097372A1 (en) | 2009-02-26 | 2010-02-23 | Compounds as bradykinin b1 antagonists |
EPPCT/EP2010/052232 | 2010-02-23 | ||
PCT/EP2011/052512 WO2011104203A1 (en) | 2010-02-23 | 2011-02-21 | Compounds as bradykinin b1 antagonists |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2790952A1 CA2790952A1 (en) | 2011-09-01 |
CA2790952C true CA2790952C (en) | 2017-07-04 |
Family
ID=43981434
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2790952A Expired - Fee Related CA2790952C (en) | 2010-02-23 | 2011-02-21 | Compounds as bradykinin b1 antagonists |
Country Status (23)
Country | Link |
---|---|
EP (1) | EP2539323B3 (en) |
JP (1) | JP5603955B2 (en) |
KR (1) | KR101843341B1 (en) |
CN (2) | CN102781916B (en) |
AP (1) | AP3218A (en) |
AR (1) | AR080249A1 (en) |
AU (1) | AU2011219885C1 (en) |
CA (1) | CA2790952C (en) |
CY (1) | CY1116182T1 (en) |
DK (1) | DK2539323T6 (en) |
ES (1) | ES2531663T7 (en) |
HK (1) | HK1175171A1 (en) |
IL (1) | IL220680A (en) |
MA (1) | MA34008B1 (en) |
MX (1) | MX2012009224A (en) |
NZ (1) | NZ601194A (en) |
PE (1) | PE20121805A1 (en) |
PL (1) | PL2539323T6 (en) |
PT (1) | PT2539323E (en) |
SG (1) | SG183336A1 (en) |
SI (1) | SI2539323T1 (en) |
TW (1) | TWI499589B (en) |
WO (1) | WO2011104203A1 (en) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE102007034620A1 (en) | 2007-07-25 | 2009-01-29 | Boehringer Ingelheim Pharma Gmbh & Co. Kg | New B1 antagonists |
ATE550321T1 (en) | 2007-08-29 | 2012-04-15 | Boehringer Ingelheim Int | NEW BRADYKININ B1 ANTAGONISTS |
KR101843341B1 (en) | 2010-02-23 | 2018-03-30 | 베링거 인겔하임 인터내셔날 게엠베하 | Compounds as bradykinin b1 antagonists |
US8937073B2 (en) | 2010-08-20 | 2015-01-20 | Boehringer Ingelheim International Gmbh | Disubstituted tetrahydrofuranyl compounds and their use as B1-receptor antagonists |
WO2012022794A1 (en) * | 2010-08-20 | 2012-02-23 | Boehringer Ingelheim International Gmbh | Pyridazin derivatives as antagonists of the bradykinin b1 receptor |
JP7376582B2 (en) * | 2018-10-22 | 2023-11-08 | イーオーイー オレオ ゲーエムベーハー | Additive for powder materials for compression into compacts |
WO2021198534A1 (en) | 2020-04-04 | 2021-10-07 | Oxurion NV | Plasma kallikrein inhibitors for use in the treatment of coronaviral disease |
WO2023148016A1 (en) | 2022-02-04 | 2023-08-10 | Oxurion NV | Biomarker for plasma kallikrein inhibitor therapy response |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6919343B2 (en) * | 2002-02-08 | 2005-07-19 | Merck & Co., Inc. | N-biphenyl(substituted methyl) aminocycloalkane-carboxamide derivatives |
CA2495914A1 (en) * | 2002-08-29 | 2004-03-11 | Merck & Co., Inc. | N-biarylmethyl aminocycloalkanecarboxamide derivatives |
EP1654232A4 (en) * | 2003-08-07 | 2008-11-12 | Merck & Co Inc | Sulfonyl substituted n-(biarylmethyl) aminocyclopropanecarboxamides |
JP2007526311A (en) | 2004-03-02 | 2007-09-13 | メルク エンド カムパニー インコーポレーテッド | Aminocyclopropanecarboxamide derivatives as bradykinin antagonists |
AU2006245770A1 (en) | 2005-05-11 | 2006-11-16 | Nycomed Gmbh | Combination of the PDE4 inhibitor roflumilast and a tetrahydrobiopterin derivative |
HUP0600809A3 (en) * | 2006-10-27 | 2008-09-29 | Richter Gedeon Nyrt | New phenylsulfamoyl-benzamide derivatives as bradykinin antagonists, process and intermediates for their preparation and pharmaceutical compositions containing them |
WO2010097372A1 (en) * | 2009-02-26 | 2010-09-02 | Boehringer Ingelheim International Gmbh | Compounds as bradykinin b1 antagonists |
KR101843341B1 (en) | 2010-02-23 | 2018-03-30 | 베링거 인겔하임 인터내셔날 게엠베하 | Compounds as bradykinin b1 antagonists |
-
2011
- 2011-02-21 KR KR1020127021929A patent/KR101843341B1/en active IP Right Grant
- 2011-02-21 NZ NZ601194A patent/NZ601194A/en not_active IP Right Cessation
- 2011-02-21 PL PL11705858T patent/PL2539323T6/en unknown
- 2011-02-21 SI SI201130402T patent/SI2539323T1/en unknown
- 2011-02-21 WO PCT/EP2011/052512 patent/WO2011104203A1/en active Application Filing
- 2011-02-21 MA MA35153A patent/MA34008B1/en unknown
- 2011-02-21 ES ES11705858.6T patent/ES2531663T7/en active Active
- 2011-02-21 AP AP2012006330A patent/AP3218A/en active
- 2011-02-21 AU AU2011219885A patent/AU2011219885C1/en not_active Ceased
- 2011-02-21 PT PT117058586T patent/PT2539323E/en unknown
- 2011-02-21 MX MX2012009224A patent/MX2012009224A/en active IP Right Grant
- 2011-02-21 SG SG2012060620A patent/SG183336A1/en unknown
- 2011-02-21 CN CN201180009563.1A patent/CN102781916B/en not_active Expired - Fee Related
- 2011-02-21 DK DK11705858.6T patent/DK2539323T6/en active
- 2011-02-21 CA CA2790952A patent/CA2790952C/en not_active Expired - Fee Related
- 2011-02-21 CN CN201410206948.2A patent/CN103980258B/en not_active Expired - Fee Related
- 2011-02-21 EP EP11705858.6A patent/EP2539323B3/en active Active
- 2011-02-21 JP JP2012553341A patent/JP5603955B2/en active Active
- 2011-02-21 PE PE2012001250A patent/PE20121805A1/en not_active Application Discontinuation
- 2011-02-22 TW TW100105851A patent/TWI499589B/en active
- 2011-02-22 AR ARP110100542A patent/AR080249A1/en not_active Application Discontinuation
-
2012
- 2012-06-28 IL IL220680A patent/IL220680A/en not_active IP Right Cessation
-
2013
- 2013-02-26 HK HK13102358.7A patent/HK1175171A1/en not_active IP Right Cessation
-
2015
- 2015-03-02 CY CY20151100211T patent/CY1116182T1/en unknown
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CA2790952C (en) | Compounds as bradykinin b1 antagonists | |
CA2994688C (en) | 1,3,4-oxadiazole derivative compounds as histone deacetylase 6 inhibitor, and the pharmaceutical composition comprising the same | |
JP6117816B2 (en) | Aminopyrimidine derivatives as LRRK2 modulators | |
CA2125236C (en) | Fungicidal optically active 2-imidazolin-5-one and 2-imidazoline-5-thione derivatives | |
ES2394126T3 (en) | Pyrimidine derivatives useful for the treatment of inflammatory or allergic conditions | |
JP6820282B2 (en) | Sulfonamide derivative and pharmaceutical composition containing it | |
EP3725787B1 (en) | Piperidine derivatives as hdac1/2 inhibitors | |
CN101098852A (en) | Glycogen phosphorylase inhibitor compounds and pharmaceutical compositions thereof | |
AU2013326429B2 (en) | N-prop-2-ynyl carboxamide derivatives and their use as TRPA1 antagonists | |
CA2737038A1 (en) | Amide compounds, compositions and uses thereof | |
CA2700051A1 (en) | Small molecule bradykinin b1 receptor antagonists | |
JP6591698B2 (en) | Novel N-[(pyrimidinylamino) propanyl]-and N-[(pyrazinylamino) propanyl] arylcarboxamides | |
EP1976851A2 (en) | Phenoxy-substituted pyrimidines as adenosine receptor antagonists | |
WO2011024001A1 (en) | Np-1 antagonists and their therapeutic use | |
CA2741400A1 (en) | Sulfonamide derivative metabotropic glutamate r4 ligands | |
AU2006237365A1 (en) | 2,3 substituted pyrazine sulfonamides as inhibitors of CRTH2 | |
MX2007015606A (en) | New azetidine derivatives as neurokinin receptor antagonists for the treatment of gastrointestinal diseases. | |
EP3297992B1 (en) | Heterocyclicalkyl derivative compounds as selective histone deacetylase inhibitors and pharmaceutical compositions comprising the same | |
JP2023036991A (en) | Amine-substituted heterocyclic compounds as ehmt2 inhibitors, salts thereof, and methods of synthesis thereof | |
JP2007513181A (en) | Novel M3 muscarinic acetylcholine receptor antagonist | |
ES2373335T3 (en) | DERIVATIVES OF 1 - [(4- [BENZOIL (METHYL) AMINO] -3- (PHENYL) BUTIL] AZETIDINE FOR THE TREATMENT OF GASTROINTESTINAL DISORDERS 1. | |
CA2446365A1 (en) | Sulfonamides | |
JP2014501266A (en) | New biarylamide derivatives | |
Yu et al. | Structure optimization and preliminary bioactivity evaluation of N-hydroxybenzamide-based HDAC inhibitors with Y-shaped cap | |
WO2011071840A1 (en) | Inhibitors of diacylglycerol acyltransferase |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |
Effective date: 20160218 |
|
MKLA | Lapsed |
Effective date: 20200221 |