CA2584576C - Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification - Google Patents

Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification Download PDF

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Publication number
CA2584576C
CA2584576C CA2584576A CA2584576A CA2584576C CA 2584576 C CA2584576 C CA 2584576C CA 2584576 A CA2584576 A CA 2584576A CA 2584576 A CA2584576 A CA 2584576A CA 2584576 C CA2584576 C CA 2584576C
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CA
Canada
Prior art keywords
stem
pcr
reagent
primer
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2584576A
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English (en)
French (fr)
Other versions
CA2584576A1 (en
Inventor
Lawrence J. Wangh
John Rice
J. Aquiles Sanchez
Kenneth Pierce
Jesse Salk
Arthur Reis
Cristina Hartshorn
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Brandeis University
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Brandeis University
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Publication of CA2584576A1 publication Critical patent/CA2584576A1/en
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Publication of CA2584576C publication Critical patent/CA2584576C/en
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/186—Modifications characterised by incorporating a non-extendable or blocking moiety
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/30—Oligonucleotides characterised by their secondary structure
    • C12Q2525/301—Hairpin oligonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/137—Concentration of a component of medium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/107—Probe or oligonucleotide ligation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2584576A 2004-10-18 2005-10-17 Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification Expired - Fee Related CA2584576C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US61967004P 2004-10-18 2004-10-18
US60/619,670 2004-10-18
PCT/US2005/037668 WO2006044995A1 (en) 2004-10-18 2005-10-17 Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification

Publications (2)

Publication Number Publication Date
CA2584576A1 CA2584576A1 (en) 2006-04-27
CA2584576C true CA2584576C (en) 2015-12-08

Family

ID=36203307

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2584576A Expired - Fee Related CA2584576C (en) 2004-10-18 2005-10-17 Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification

Country Status (14)

Country Link
US (2) US7517977B2 (no)
EP (1) EP1802774B1 (no)
JP (2) JP5165376B2 (no)
KR (1) KR20070090158A (no)
CN (1) CN101076608B (no)
AT (1) ATE541055T1 (no)
AU (1) AU2005295299B2 (no)
BR (1) BRPI0516619B1 (no)
CA (1) CA2584576C (no)
ES (1) ES2380775T3 (no)
NO (1) NO341716B1 (no)
NZ (1) NZ554701A (no)
RU (1) RU2495046C2 (no)
WO (1) WO2006044995A1 (no)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1802774B1 (en) * 2004-10-18 2012-01-11 Brandeis University Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification
EP1943348B1 (en) * 2005-10-03 2013-01-02 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids
US7833716B2 (en) 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
US7972786B2 (en) * 2006-07-07 2011-07-05 Brandeis University Detection and analysis of influenza virus
CN102421918B (zh) 2009-03-12 2016-01-20 布兰代斯大学 用于pcr的试剂和方法
EP2412718B1 (en) 2009-03-26 2016-10-12 Xiamen Amoy Diagnostics Co., Ltd Loop-shaped primer employed in nucleic acid amplification and the use thereof
US8815514B2 (en) * 2009-07-02 2014-08-26 Co-Diagnostics, Inc. Detection primers for nucleic acid extension or amplification reactions
US9353407B2 (en) 2009-10-21 2016-05-31 Brandeis University Methods, kits and reaction mixtures for analyzing single-stranded nucleic acid sequences
US9169514B2 (en) 2010-12-03 2015-10-27 Brandeis University Detecting nucleic acid variations within populations of genomes
WO2013154898A1 (en) * 2012-04-12 2013-10-17 Ong, Jennifer Synthetic nucleic acids for polymerization reactions
ES2730675T3 (es) * 2013-01-23 2019-11-12 Univ Brandeis Reactivos para mejorar la precisión de la PCR
US20160355881A1 (en) * 2013-07-24 2016-12-08 Brandeis University DNA Methylation Analysis
WO2015038869A1 (en) * 2013-09-13 2015-03-19 Brandeis University Modified polymerase compositions, methods and kits
JP2017515484A (ja) * 2014-05-19 2017-06-15 ウィリアム マーシュ ライス ユニバーシティＷｉｌｌｉａｍ Ｍａｒｓｈ Ｒｉｃｅ Ｕｎｉｖｅｒｓｉｔｙ 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
US9477198B2 (en) 2014-09-03 2016-10-25 Samsung Electronics Co., Ltd. Cleaning device and electrophotographic image forming apparatus having the same
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
JP2017537648A (ja) 2014-12-19 2017-12-21 ブランデイズ ユニバーシティー ミスプライミング防止試薬
WO2016138080A1 (en) 2015-02-24 2016-09-01 Trustees Of Boston University Protection of barcodes during dna amplification using molecular hairpins
US20190309349A1 (en) * 2016-05-18 2019-10-10 Integrated Nano-Technologies, Inc. Method for detection of a pcr product
KR102461735B1 (ko) 2016-09-23 2022-11-01 알베오 테크놀로지스 인크. 분석물을 검출하기 위한 방법 및 조성물
MX2021007311A (es) * 2018-12-20 2021-09-21 Alveo Tech Inc Metodos y composiciones para reducir la amplificacion no especifica en reacciones de amplificacion isotermica.
KR102275038B1 (ko) * 2019-12-11 2021-07-08 (주) 제노텍 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트

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US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5066584A (en) * 1988-09-23 1991-11-19 Cetus Corporation Methods for generating single stranded dna by the polymerase chain reaction
US5693502A (en) * 1990-06-11 1997-12-02 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5874557A (en) * 1990-06-11 1999-02-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5811300A (en) * 1992-12-07 1998-09-22 Ribozyme Pharmaceuticals, Inc. TNF-α ribozymes
US5925517A (en) * 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
ATE420202T1 (de) * 1995-06-07 2009-01-15 Gilead Sciences Inc Nukleinsäure liganden die dna-polymerase binden und inhibieren
US6183967B1 (en) * 1995-06-07 2001-02-06 Nexstar Pharmaceuticals Nucleic acid ligand inhibitors to DNA polymerases
US5773258A (en) * 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
CA2252048C (en) * 1996-04-12 2008-03-11 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
US6117635A (en) * 1996-07-16 2000-09-12 Intergen Company Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US6787308B2 (en) * 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
EP1196559B1 (en) * 1999-07-02 2006-07-05 Invitrogen Corporation Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis
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EP1802774B1 (en) * 2004-10-18 2012-01-11 Brandeis University Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification
EP1943348B1 (en) * 2005-10-03 2013-01-02 Life Technologies Corporation Compositions, methods, and kits for amplifying nucleic acids

Also Published As

Publication number Publication date
JP2013063070A (ja) 2013-04-11
NO20072507L (no) 2007-07-06
BRPI0516619B1 (pt) 2016-02-10
US20090226973A1 (en) 2009-09-10
AU2005295299B2 (en) 2010-10-21
AU2005295299A1 (en) 2006-04-27
JP2008516613A (ja) 2008-05-22
EP1802774B1 (en) 2012-01-11
CA2584576A1 (en) 2006-04-27
JP5165376B2 (ja) 2013-03-21
CN101076608B (zh) 2013-04-17
RU2007118546A (ru) 2008-11-27
KR20070090158A (ko) 2007-09-05
EP1802774A1 (en) 2007-07-04
US7517977B2 (en) 2009-04-14
ATE541055T1 (de) 2012-01-15
NZ554701A (en) 2010-03-26
US20060177842A1 (en) 2006-08-10
WO2006044995A1 (en) 2006-04-27
RU2495046C2 (ru) 2013-10-10
EP1802774A4 (en) 2008-10-01
NO341716B1 (no) 2018-01-08
BRPI0516619A (pt) 2008-09-16
ES2380775T3 (es) 2012-05-18
CN101076608A (zh) 2007-11-21

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