CA2322207A1 - Meta-azacyclic amino benzoic acid compounds and derivatives thereof being integrin antagonists - Google Patents

Abstract

The present invention is directed to compounds of formula (1) and pharmaceutically acceptable salts and isomers thereof useful as .alpha.v.beta.3 integrin antagonists.

Description

META-AZACYCLIC AMINO BENZOIC ACID COMPOUNDS AND DERIVATIVES
THEREOF BEING INTGRIN ANTAGONISTS
t~ield of the invention The present invention relates to pharmaceutical agents (compounds) which are useful as a"~3 integrin antagonists and as such are useful in pharmaceutical compositions and in methods for treating conditions mediated by a"~ by inhibiting or antagonizing a"~ integrins.
Background of the Invention is Integrins are a group of cell surface glyooproteins which mediate cell adhesion end therefore are useful mediators of cell adhesion interactions which occur during various biological processes. Integrins are heterodimers composed of noncovaiently linked a and a polypeptide subunits. Currently eleven different a subunits have been ident~ed and 2o six different (i subunits have been identified. The various a subunits can combine with various ~ subunits to form distinct integrins.
The integrin identified as a"~3 (also known as the vitronectin receptor) has been identified as an integrin which plays a role in various conditions or disease states including tumor metastasis, solid tumor growth 2s (neoplasia), osteoporosis, Paget's disease, humoral hypercaicemia of malignancy, angiogenesis, including tumor angiogenesis, retinopathy, including macular degeneration, arthritis, including rheumatoid >~rthritis, periodontal disease, psoriasis and smooth muscle cell migration (e.g.
restenosis). Additionally, it has been found that such agents would be 3o useful as antivirals, antifungals and antimicrobials. Thus, compounds which selectively inhibit or antagonize a"~3 would be beneficial for treating such conditions.

It has been shown that the ayp3 integrin and other a~ containing integrins bind to a number of Arg-Gly-Asp (RGD) containing matrix macromolecules. Compounds containing the RGD sequence mimic extracellular matrix ligands so as to bind to cell surface receptors.
s However, it is also known that RGD peptides in general are non-selective for RGD dependent integrins. For example, most RGD peptides which bind to a"~~ also bind to a"a5, a~~, and a"bp5. Antagonism of platelet apb~9 (also known as the fibrinogen receptor) is known to block platelet aggregation in humans. In order to avoid bleeding side-effects when to treating the conditions or disease states associated with the integrin a"~3, it would be beneficial to develop compounds which are selective antagonists of a"p~ as opposed to a"~~.
Tumor cell invasion occurs by a three step process: 1 ) tumor cell attachment to extracellular matrix; 2) proteolytic dissolution of the matrix;
1s and 3) movement of the cells through the dissolved barrier. This process can occur repeatedly and can result in metastases at sites distant from the original tumor.
Seftor et al. (Proc. Natl. Aced. Sci. USA, Vol. 89 (1992) 1557-1561 ) have shown that the a"p3 integrin has a biological function in melanoma 2o cell invasion. Montgomery et al., (Proc. Natl. Acad. Sci. USA, Vol. 91 (1994) 8856-60) have demonstrated that the integrin a"~3 expressed on human melanoma cells promotes a survival signal, protecting the cells from apoptosis. Mediation of the tumor cell metastatic pathway by interterence with the a"(iy integrin cell adhesion receptor to impede tumor 2s metastasis would be beneficial.
Brooks et al. (Cell, Vol. 79 (1994) 1157-1164) have demonstrated that antagonists of a"p, provide a therapeutic approach for the treatment of neoplasia (inhibition of solid tumor growth) since systemic administration of a"~i3 antagonists causes dramatic regression of various histologically 3o distinct human tumors.
The adhesion receptor integrin a~~i3 was identified as a marker of angiogenic blood vessels in chick and man and therefore such receptor plays a critical role in engiogenesis or neovascularization. Angiogenesis is characterized by the invasion, migration and proliferation of smooth muscle and endothelial cells. Antagonists of a"~ inhibit this process by selectively promoting apoptosis of cells in neovasculature. The growth of s new blood vessels, or angiogenesis, also contributes to pathological conditions such as diabetic retinopathy and macular degeneration (Adonis et al., Amer. J. Ophthal., Vol. 118, (1994) 445-450) and fieumatoid arthritis (Peacock et al., J. Exp. Med., Vol. 175, (1992), 1135-1138).
Therefore, a"~ antagonists would be useful therapeutic targets for treating to such conditions associated with neovascularization (Brooks et al., Science, Vol. 2fi4, (1994), 5fi9-571 ).
It has been reported that the cell surface receptor a"[i~ is the major integrin on osteoclasts responsible for attachment to bone. Osteocfasts cause bone resorption and when such bone resorting activity exceeds is bone forming activity it results in osteoporosis (a loss of bone), which leads to an increased number of bone fractures, incapacitation and increased mortality. Antagonists of a"~3 have been shown to be potent inhibitors of osteoclastic activity both in vitro [Sato et al., J. Cell.
Biol., Vol.
111 (1990) 1713-1723] and in vivo [Fisher et al., Endocrinology, Vol. 132 20 (1993) 1411-1413]. Antagonism of a"[i3 leads to decreased bone resorption and therefore restores a normal balance of bone forming and resoround bottoming activity. Thus it would be beneficial to provide antagonists of osteoclast a"~3 which are effective inhibitors of bone resorption and therefore are useful in the treatment or prevention of 25 osteoporosis.
The role of the a"~~ integrin in smooth muscle cell migration also makes it a therapeutic target for prevention or inhibition of neointimal hyperplasia which is a leading cause of restenosis after vascular procedures (Choi et al., J. Vasc. Surg. Vol. 19(1 ) (1994) 125-34).
3o Prevention or inhibition of neointimal hyperplasia by pharmaceutical agents to prevent or inhibit restenosis would be beneficial.

. .. .... .. .... .. ..
.. .. ~ . . . . . . . .
. . . . . . . . . .. .
. . . . . . . . . . . . .
. . . . . . .. . . .. .
. . 4 . .. . .. .. .. ..
lNhite (Current Biology, Vol. 3(9)(1993) 59fi-599) has reported that adenovirus uses a"~., for entering host cells. The integrin appears to be required for endocytosis of the virus particle and may be required for penetration of the viral genome into the host cell cytoplasm. Thus .compounds which inhibit a"~, would find usefulness as antiviral agents.
V11097108145 discloses meta-guanidine-, urea-, thiourea- and azacyclic aminobenzoic acid derivatives of the formula (I) wherein A is -N'' T~ (b) R~
tc) --N~,~H"'~ ca) R. R, which are usefule as a"(33 integrin antagonists.
J. Med. Ghem. 40, 930 (1997), J. Med. Chem. 40, 920 (1997) and Antiv. Chem. Chemother. 8, 463 (1997) are concerned with the discovery of H(V-1 integrase inhibitors by specific pharmacophore searching.
Exp. Opin. Ther. Patents 8, 633 (1998) has been published after the presently claimed priority and is concerned with the development that integrin antagonists could be used to inhibit metastasis.

' _ ~

w ~

~ 1 ~p f Summary of the Invention The present invention relates to compound of the following general formula .. O
n N
HO

to -- .
wherein X and Y are the same or different halo group and pharmaceutically acceptable salts thereof.
The compounds described above can exist in various isomeric forms and all such isomeric forms are meant to be included. Tautomeric ~s forms are also included as well as pharmaceutically acceptable salts of such isomers and tautomers.
More specifically, the present invention relates to the following compounds:
H H ~ H
.._~ N ~~ ~ " ~ ~ 'OHR~

H

.
0 _ _OH

H H H
( ~ COZR.
H N p , ' pH
H H H
/ I ~'~ Co2R .
~N ~ O ~ pH
off ~ I
Br CI
H H H
NI~ COzR .
N ,~ ~ H O OH ~ M
I, OH C ~~. I
s H ~. O H
N CpZR
HO N O / OH Nn I
Br ~ Br H H O H
NYN , ~N CSR. .
~N ~ ( H p / OH
OH ~ I
t CI
to H I H
H~ C W

HO
Bf OzR
iH ~ (~
s ~R
H
'~ COZR , p / OH ~ C~
CI \' Br .. .. 0 R
C~
H
N NH CSR
H
N O OH , (XV) and HO
s Br W I
NH COZR
HO N O ~ OH
wherein R is H or alkyl;
~o or pharmaceutically acceptable salts thereof.
It is another object of the invention to provide pharmaceutical compositions comprising compounds described above. Such compounds and compositions are useful in selectively inhibiting or antagonizing the integrin and therefore in another embodiment the present invention reiates is to a method of selectively inhibiting or antagonizing the a"~3 integrin.
The __ ..

W.O 99144994 PCT/US99103281 invention further involves treating or inhibiting pathological conditions associated therewith such as osteoporosis, humoral hypercalcemia of malignancy, Paget's disease, tumor metastasis, solid tumor growth (neopiasia), angiogenesis, including tumor angiogenesis, retinopathy s including diabetic retinopathy and macular degeneration, arthritis, including fieumatoid arthritis, periodontal disease, psoriasis, smooth muscle cell migration and restenosis in a mammal in need of such treatment. Additionally, such pharmaceutical agents are useful as antiviral agents, and antimicrobials.
io Detailed Description The present invention relates to a class of compounds represented by Formulae I-XVI, described above.
is Preferred embodiments of the present invention are compounds of the following formulae H I
02H, IH
HO
r .. .. 0 H
'COSH _ OH a CI' ~ CI

~02H
~H

iH
H
~C02H
O OH
C ~ I
.. .. 0 H
N
'C02H
OH
Br' ~ Br H I H

HO N O ~ OH
i ~ CI

.. .. 0 ..

H H H
H~ C02H.
N ~ ~ '0I OH ~ C
H /
OH I ~ Br H
~H .
N pH
HO

H .. 0 ~H
N ~H ~ G~
HO
s I
.. .. 0 H~ _NH C02H.
O ~, OH ~ ~) Ct ~ CI
.. .. O
a~
N'~ C02H
H O / OH
CI ~ Br N~ ~~~ C02H
H 0 . ,OH W
~02H
OH
"02H
..
CH ; (~ and .." I
s H r, O
H~ 'NH C02H
N p / OH
HO
The invention further relates to pharmaceutical compositions to containing therapeutically effective amounts of the compounds described above.
The invention also relates to a method of selectively inhibiting or antagonizing the a"~i3 integrin and more specifically relates to a method of inhibiting bone resorption, periodontal disease, osteoporosis, humoral is hypercalcemia of malignancy, Paget's disease, tumor metastasis, solid n __ .. O
.. .. O

tumor growth (neopiasia), angiogenesis, including tumor angiogenesis, retinopathy including diabetic retinopathy and macular degeneration, arthritis, including rheumatoid arthritis, smooth muscle cell migration and restenosis by administering a therapeutically effective amount of a compound described above to achieve such inhibition together with a pharmaceutically acceptable carrier.
The following is a list of definitions of various terms used herein:
As used herein, the terms "alkyl" or "lower alkyl" refer to a straight chain or branched chain hydrocarbon radicals having from about 9 to to about 10 carbon atoms, and more preferably 1 to about 6 carbon atoms.
Examples of such alkyl radicals are methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, t-butyl, pentyl, neopentyl, hexyi, isohexyl, and the like.
As used herein the term "halo" or "halogen" refers to bromo, chloro, is or iodo.
As used herein the term "haloalkyl" refers to alkyl groups as defined above substituted with one or more of the same or different halo groups at one or more carbon atom. Examples of haloalkyi groups include trifiuoromethyl, dichloroethyl, fiuoropropyl and the like.
2o The term "composition" as used herein means a product which results from the mixing or combining of more than one element or ingredient.
The term "pharmaceutically acceptable carrier", as used herein means a pharmaceutically-acceptable material, composition or vehicle, 2s such as a liquid or solid filler, diluent, excipient, solvent or encapsulating material, involved in carrying or transporting a chemical agent.
The term "therapeutically effective amount" shall mean that amount of drug or pharmaceutical agent that will elicit the biological or medical response of a tissue, system or animal that is being sought by a 3o researcher or clinician.
The following is a list of abbreviations and the corresponding meanings as used interchangeably herein:
t2 'H-NMR = proton nuclear magnetic resonance AcOH = acetic acid Ar = argon CH3CN = acetonitriie s CHN analysis = carbonlhydrogeNnitrogen elemental analysis CHNCI analysis = carbonfiydrogeNnitrogeNchlorine elemental analysis CHNS analysis = carboNhydrogeNnitrogenlsulfur elemental analysis 1o DI water = deionized water DMA = QI,_N-dimethyiacetamide DMAP = 4-(~I,N_-dimethylamino)pyridine DMF = ~l,~J-dimethylformamide EDCI = 1-(3-dimethylaminopropyi)-3-ethylcarbodiimide is hydrochloride EtOAc = ethyl acetate .
EtOH = ethanol FAB MS = fast atom bombardment mass spectroscopy g = grams) 2o HOST =1-hydroxybenzotriazole hydrate HPLC = high performance liquid chromatography IBCF = isobutylchloroformate KSCN = potassium thiocyanate L = liter 2s LiOH = lithium hydroxide MEM = methoxyethoxymethyl MEMCI = methoxyethoxymethyl chloride MeOH = methanol mg = milligram 3o MgSO,, = magnesium sulfate ml = milliliter mL = milliliter MS = mass spectroscopy MTBE = methyl tert-butyl ether 3s N2 = nitrogen NaHCOa = sodium bicarbonate NaOH = sodium hydroxide Na2S0~ = sodium sulfate NMM = N-methylmorpholine 4o NMP = N-methyl pyrrolidinone NMR = nuclear magnetic resonance P20s = phosphorous pentoxide PTSA = para-toluenesulfonic acid RPHPLC = reverse phase high performance liquid 4s chromatography RT = room temperature TFA = trifluoroacetic acid THF = tetrahydrofuran TMS = trimethylsilyl O = heating the reaction mixture The compounds described above can exist in various isomeric forms and all such isomeric fom~s are meant to be included. Tautomeric forms are also included as well as pharmaceutically acceptable salts of such isomers and tautomers.
In the structures and formulas herein, a bond drawn across a bond of a ring can be to any available atom on the ring.
to The term "pharmaceutically acceptable salt" refers to a salt prepared by contacting a compound described above with an acid whose anion is generally considered suitable for human consumption. Examples of pharmacologically acceptable salts include the hydrochloride, hydrobromide, hydroiodide, sulfate, phosphate, acetate, propionate, is lactate, maleate, malate, suxinate, tartrate salts arid the like. All of the pharmacologically acceptable salts may be prepared by conventional means. (See Berge et al.,,~ Pharm. Sci.. fifi(1), 1-19 (1977) for additional examples of pharmaceutically acceptable salts.) For the selective inhibition or antagonism of a"~3 integrins, 2o compounds of the present invention may be administered orally, parenteraliy, or by inhalation spray, or topically in unit dosage formulations containing conventional pharmaceutically acceptable carriers, adjuvants and vehicles, The term parenteral as used herein includes, for example, subcutaneous, intravenous, intramuscular, intrastemal, infusion 25 techniques or intraperitonally.
The compounds of the present invention are administered by any suitable route in the form of a pharmaceutical composition adapted to such a route, and in a dose effective for the treatment intended. Therapeutically effective doses of the compounds required to prevent or arrest the 3o progress of or to treat the medical condition are readily ascertained by one of ordinary skill in the art using preclinical and clinical approaches familiar to the medicinal arts.

WO 99144994 PCTNS99/0328~
Accordingly, the present invention provides a method of treating conditions mediated by selectively inhibiting or antagonizing the a"~3 cell surface receptor which method comprises administering a therapeutically effective amount of a compound selected from the class of compounds s described above, wherein one or more compounds is administered in association with one or more non-toxic, pharmaceutically acceptable carriers andlor diluents andlor adjuvants (collectively referred to herein as "carrier' materials) and if desired other active ingredients. More spec~cally, the present invention provides a method for inhibition of the ~o a"(33 cell surface receptor. Most preferably the present invention provides a method for inhibiting bone resorption, treating osteoporosis, inhibiting humoral hypercalcemia of malignancy, treating Paget's disease, inhibiting tumor metastasis, inhibiting neoplasia (solid tumor growth), inhibiting angiogenesis including tumor angiogenesis, treating diabetic retinopathy 1s and macular degeneration, inhibiting arthritis, psoriasis and periodontal disease, and inhibiting smooth muscle cell migration including restenosis.
Based upon standard laboratory experimental techniques and procedures well known and appreciated by those skilled in the art, as well as comparisons with compounds of known usefulness, the compounds 2o described above can be used in the treatment of patients suffering from the above pathological conditions. One skilled in the art will recognize that selection of the most appropriate compound of the invention is within the ability of one with ordinary skill in the art and will depend on a variety of factors including assessment of results obtained in standard assay and 2s animal models.
Treatment of a patient afflicted with one of the pathological conditions comprises administering to such a patient an amount of compound described above which is therapeutically effective in controlling the condition or in prolonging the survivability of the patient beyond that 3o expected in the absence of such treatment. As used herein, the term "inhibition" of the condition refers to slowing, interrupting, arresting or stopping the condition and does not necessarily indicate a total elimination of the condition. It is believed that prolonging the survivability of a patient, beyond being a sign~cant advantageous effect in and of itself, also indicates that the condition is beneficially controlled to some extent.
As stated previously, the compounds of the invention can be used in a variety of biological; prophylactic or therapeutic areas. It is contemplated that these compounds are useful in prevention or treatment of any disease state or condition wherein the a"p3 integrin plays a role.
The dosage regimen for the compounds andlor compositions containin8 the compounds is based on a variety of factors, including the to type, age, weight, sex and medical condition of the patient; the severity of the condition; the route of administration; and the activity of the particular compound employed. Thus the dosage regimen may vary widely. Dosage levels of the order from about 0.01 mg to about 1000 mg per kilogram of body weight per day are useful in the treatment of the above-indicated is conditions, and more preferably from about 0.01 mg to about 100 mg per kg of body weight per day.
The active ingredient administered by injection is formulated as a composition wherein, for example, saline, dextrose or water may be used as a suitable carrier. A suitable daily dose would typically be about 0.01 to 20 10 mglkg body weight injected per day in multiple doses depending on the factors listed above.
For administration to a mammal in need of such treatment, the compounds in a therapeutically effective amount are ordinarily combined with one or more adjuvants appropriate to the indicated route of 2s administration. The compounds may be admixed with lactose, sucrose, starch powder, cellulose esters of alkanoic acids, cellulose alkyl esters, talc, stearic acid, magnesium stearate, magnesium oxide, sodium and calcium sans of phosphoric and sulphuric acids, gelatin, acacia, sodium alginate, polyvinylpyrrolidone, andlor polyvinyl alcohol, and tableted or 3o encapsulated for convenient administration. Alternatively, the compounds may be dissolved in water, polyethylene glycol, propylene glycol, ethanol, com oil, cottonseed oil, peanut oil, sesame oil, benzyl alcohol, sodium chloride, andlor various buffers. Other adjuvants and modes of administration are well and widely known in the pharmaceutical art.
The pharmaceutical compositions useful in the present invention may be subjected to conventional pharmaceutical operations such as sterilization andlor may contain conventional pharmaceutical adjuvants such as preservatives, stabilizers, wetting agents, emulsifiers, buffers, etc.
The general synthetic sequences for preparing the compounds useful in the present invention are outlined in Schemes I-Ill. Both an explanation of, and the actual procedures for, the various aspects of the 1o present invention are described where appropriate. The following Schemes and Examples are intended to be merely illustrative of the present invention, and not limiting thereof in either scope or spirit. Those with skill in the art will readily understand that known variations of the conditions and processes described in the Schemes and Examples can be is used to synthesize the compounds of the present invention.
Unless otherwise indicated all starting materials and equipment employed were commercially available.

SCHEMEI
~H COZH
O~H
NH~SCN, HC1, H=O ~ ~ S
1. NH~INhfwCl' ~ W
OH ~ HO \ NHy ~ HO H NHZ
HO
D
Me!
H
~NH C02H
HO--( ~~-NH NHI NHz , SMe ~N
COpH ~ HCI ~ ~ ~ ~ HI
O HO W Ni 'NH2 HO 2. dilute HCI
Scheme I illustrates methodology useful for preparing the tetrahydropyrimidinobenzoic acid portion of the present invention which can be coupled to a gly-p-amino acid ester. Briefly, in Scheme I, 3,5-to dihydroxybenzoic acid is converted to 3-amino-5-hydroxy-benzoic acid using the procedure described in Austr. J. hem., 34 (6), 1319-24 (1981 ).
The product is reacted with ammonium thiocyanate in hot dilute hydrochloric acid to give 3-thiourea-5-hydroxybenzoic acid after normal work-up. This thiourea intermediate is converted to the S-methyl is derivative by reaction with methyl iodide in ethanol at reflux. 7,3-diamino-2-hydroxypropane is reacted with this resulting intermediate in hot DMA.
Upon cooling precipitate forms and the zwitterionic product is isolated by filtration. The HCt salt may be obtained by lyophilizing from dilute hydrochloric acid. Alternatively, the product may be isolated from the 20 original reaction mixture by removing volatiies and concentrating. The resulting product is taken up in water and pH adjusted to about 5-7 where zwitterionic product precipitates and is isolated by filtration. The HCI salt Is may be obtained as previously stated or by simply dissolving in dilute hydrochloric acid and concentrating to a solid and drying.

SCHEME IA
NH
HO ~CSZ E ~~ HO ~S --~ HO SMe p NH p NH
N
O~O

1. DMA
/ ~ / p 1 ~. n~-~rN~t,a ~ ~ Z. Ha HO OH p HO NH2 2. FtCUh4Z0 p NH
HO ~>-NH
N
C02H ~ HCt HO
BOGlO CH~aZ, DMA, Et3N
N
HO ~~--SMe Scheme L4 illustrates methodology useful for preparing the tetrahydropyrimidinobenzoic acid portion of the present invention which can be coupled to a gly-p-amino acid ester. Briefly, in Scheme IA 1,3-diamino-2-hydroxypropane is reacted with carbon disulfide in an s appropriate solvent such as ethanol - water, refiuxed, cooled, hydrochloric acid added, refluxed again, cooled and the product, 5-hydroxytetrahydropyrimidine-2-thione harvested by filtration and dried.
This cyclic thiourea intermediate is converted to the S-methyl derivative by reaction of thione and methyl iodide in ethanol at reflux. The desired 2-io methylthioether-5-hydroxypyrimidine hydroiodide is readily isolated by removing volatiles at reduced pressure. Thus, 2-methylthioether-5-hydroxypyrimidine hydroiodide in methylene chloride : DMA (about 10:1 ) and an equivalent of triethylamnine are cooled to about ice-bath temperature and an equivalent of di tert-butyl Bicarbonate (BOC
is anhydride) added. Conventional work-up gives the BOC-2-methylthioether-5-hydroxypyrimidine as an oil.
3,5-dihydroxybenzoic acid is converted to 3-amino-5-hydroxy-benzoic acid using the procedure of Aust. J. Chem., 34 (6), 1319-24 (1981 ).
2o The final desired product, 3-hydroxy-5-((5-hydroxy-1,4,5,6-tetrahydro-2-pyrimidinyl)amino]benzoic acid hydrochloride salt, is prepared by reacting 80C-2-methylthioether-5-hydroxypyrimidine and 3-amino-5-hydroxy-benzoic acid in hot DMA. Upon cooling, a precipitate forms and zwitterionic product isolated by filtration. The HCI salt can be obtained by 2s lyophilizing from dilute hydrochloric acid, for example.

SCHEME Il O
~ AczOlE1'3N I / X TMSzNUITHF~
X
Y
xQ or N-7GSuccinimiab a other souoe otx" EtOH, HCI
O H C02Et NHZ
OH
~ OH
~ HCI
X
O
0,,, O NMM
H
O
O
~ HCI ~~H
dioxane Y and X are halo groups Scheme II illustrates methodology useful for preparing the ethyl N-gly-amino-3-(3,5-dihalo-2-hydroxy) phenyl propionate portion of the present invention which can be coupled to the tetrahydropyrimidinobenzoic acid moiety. Briefly, 3,5-halo substituted salicylatdehydes may be s prepared by direct halogenation as, for example, would be the case where 5-bromosalicylaldehyde is slurried in acetic acid and an equivalent or more of chlorine is added to yield 3-chloro-5-bromo-2-hydroxybenzaldehyde.
Some product precipitates and can be recovered by filtration. The remainder may be recovered by diluting the filtrate with water and isolating to the precipitate. Combining the solids and drying gives 3-chloro-5-bromo-2-hydroxybenzaldehyde. 3-iodo-5-chforosalicylaldehyde may be prepared by reacting 5-chlorosalicylaldehyde with N-iodosuccinimide in DMF and subjecting the reaction mixture to usual work-up conditions. 3-iodo-5-bromosaiicylaldehyde may be prepared by reacting 5-is bromosalicylaldehyde in acetonitrile with potassium iodide and chloramine T. Work-up gives a material that when treated with hexanes gives the desired 3-iodo-5-chlorosalicylaldehyde.
Coumarins are readily prepared from salicylaldehydes using a modified Perkin reaction (e.g., Vogel's Textbook of Practical Organic 2o Chemistry, 5th Ed., 1989, p. 1040, ) . The halo-substituted coumarins are converted to 3-aminohydrocoumarins (see J.G. Rico, Tett. et., 1994, 35, fi599-6602) which are readily opened in acidic alcohol to give 3-amino-3-(3,5-halo-2-hydroxy)phenyl propanoic acid esters.
3-amino-3-(3,5-halo-2-hydroxy)phenyl propanoic acid esters are 2s converted to N-gly-3-amino-3-(3,5-halo-2-hydroxy)phenyl propanoic acid asters by reaction of Boc-N-gly-N-hydroxysuccinimide to give Boc-N-giy- 3-amino-3-(3,5-halo-2-hydroxy)phenyl propanoic acid esters that are converted to HX salts of N-gly- 3-amino-3-(3,5-halo-2-hydroxy)phenyl propanoic acid esters (wherein X is a halo group) for example, by removal 30 of the BOC-protecting group using HCI in ethanol.
The amino acid compounds used in preparing the compounds of the present invention can be prepared according to the procedures set forth herein and below and according to the methodology described and claimed in co-pending USSN Attorney Docket 3076, filed simultaneously herewith and hereby incorporated by reference.

VIiO 99/44994 PCTIUS99/03281 SCHEME III
NH
HO ~>--NH
N 1. IBCF, NMM, DMA, >d~
OZH
~ HCI
HO 2. ~
~ HCI
1. LiOH, H20 Y and X are halo groups Scheme III is illustrative of methodology useful for preparing various compounds of the present invention. 3-Hydroxy-5-((1,4,5,6-tetrahydro-5-hydroxy-2-pyrimidinyl)amino)benzoic acid is activated to s coupling using known methods. Thus, after dissolving in a suitable solvent such as DMA an equivalent of NMM is added. The reaction mixture is cooled to ice-bath temperatures and IBCF added. To the mixed anhydride intermediate is added the gly-p-amino acid ester and NMM. Upon completion of the reaction the product is purified by prep hplc and the to ester hydrolyzed to the acid by treating with a base, such as Li~H in a suitable solvent (dioxanelwater or acetonitrilelwater). Alternatively, a suitable acid, such as TFA can be used. The product is isolated by prep hplc or by isolating the zwitterion at pH 5-7 and converting to the desired salt by standard procedures.

EXAMPLE A
Preparation of HQ
s to Preparation of O

CI

CI

To a 2L round bottom flask fitted with a mechanical stirrer and to condenser was added 3, 5-dichlorosalicylaldehyde (200 g, 1.05 mol, 1 equiv.), acetic anhydride (356 g , 3.49 mot) and triethylamine (95.0 g , 0.94 mol, 0.90 equiv.). The reaction solution was heated at reflux overnight.
The dark brown reaction mixture was cooled to 50°C and water (1 L) added with stirring. After one hour the mixture was filtered and the filtrate is combined with EtOH (1 L). This mixture was heated to 45°C for one hour, cooled to room temperature, filtered and the solid (fraction A) washed with EtOH (0.5 L). The combined EtOH solutions were concentrated by rotary evaporation to an oil (fraction B). The solid from fraction A was dissolved in methyiene chloride (1.5 L) and the resulting solution passed through a 2o pad of silica gel (1300 mL volume). The resulting dark brown solution was concentrated to an oil that was triturated with hexanes (1.3 L) to give a solid that was isolated by filtration and washed (hexanes) to give substantially pure 6,8-dichiorocoumarin (163 g). A further 31 g of product was obtained by treating the oil, fraction B, in a similar fashion; the oil was W~,199/44994 PCTIUS99I03281 dissolved in methylene chloride (0.5 L) passed through a silica pad (0.5 L
volume) and triturated with hexanes. The total isolated yietd was 194 g or 86°~ yield of the brown solid.
MS and NMR were consistent with the desired structure.
s $tea 22 Preparation of H2N C02Et OH ~ HCI
CI CI
1o To a 3-neck 2L round bottom flask fitted with a mechanical stirrer was added 6,8-dichlorocoumarin (160 g, 0.74 mol) (prepared in Step 1 ) and dry THF (375 mL, Aldrich~Sure Seal). The resulting mixture was cooled to - 40°C (dry icelacetone bash) and lithium bis(trimethylsilyt)amide (0.60 mol , 800 mL of 1 M in THF) added while maintaining temperature 1s below -40 C. After the completion of the addition the cooling bath was removed . After 0.5 hour the mixture had warmed to -5°C. The reaction was quenched by addition of a solution of HCI (0.5 L of 4M in dioxane) in EtOH (1.25 L). The temperature was maintained below 0°C overnight.
The reaction mixture was concentrated to about one-half its original volume 2o and partitioned between EtOAc (3 L) and water (2L). The organic layer was washed with aqueous HCI (3 x 1 L 0.5 N HCI). The pH of the combined aqueous layers was adjusted to about 7 by addition of 10°~
aqueous NaOH and extracted with methylene chloride (3 x 2L). The combined organic layers were dried (MgSO,), filtered, and 4M HCI in 2s dioxane (210 mL) added with stirring. Upon completion of precipitation the solid was removed by filtration. The filtrate was concentrated to a small volume and methyl t-butyl ether added. The solid obtained was combined with the initially formed solid and the combined product was washed with methyl t-butyl ether, isolated by filtration and dried (vacuum oven over a weekend) to obtain the desired product (172 g, 74°~ yield).
MS and NMR were consistent with the desired structure.
to Preparation of O
H
~O~N N C Et H
O OH
CI ~ CI
To a flame-dried round bottom flask (0.5 L) equipped with magnetic io stir bar was added N-t-Boc-glycine N-hydroxysuccinimide ester (Sigma, 15.0 g, 0.055 mol), dry DMF (Aldrich Sure Seal, 200 mL) and the product from Step 2 (21.67 g, 0.055 mol) under an inert atmosphere (Ar). The reaction mixture was cooled to approximately 0°C (salt-ice bath) and N-methylmorpholine (5.58 g, 0.056 mole) and a catalytic amount of DMAP
is added and the reaction allowed to proceed overnight. The reaction mixture was concentrated to a slush, and partitioned between EtOAc (0.4L) and aqueous base (2 x 0.2 L, aqueous saturated NaHC03). The organic layer was washed consecutively with aqueous citric acid (2 x 0.2 L, 10°h wlv), again with aqueous sodium bicarbonate (2 x 0.2 L), brine and dried 20 (Na2S04). Volatiles were removed under vacuum at 55°C to give an oil (22.5 g, 92°h yield) that solidified on standing.
MS and NMR were consistent with the desired structure.
to 4 Preparation of H
H2N~N C02Et O , I OH ~ HCI

The product obtained in Step 3 was de-protected to give the amine hydrochloride salt using the following procedure. To the product from Step 3 (14.0 g, 0.032 mole) in a flame-dried round bottom flask (0.1 L) with stir bar was added dry dioxane (40 mL). To this was added 4.0 N HCI in s dioxene (2 equiv., fi:32 mL) at 0°C and the reaction allowed to proceed until gas evolution ceased and the reaction was complete. Volatiles were removed under vacuum and the residue triturated with diethyl ether (50 mL). Solids were collected by filtration and washed with ether and dried to give the desired product (12.5 g).
~o MS and NMR were consistent with the desired structure.

Vv0 99/44994 PCTIUS99/03281 EXAMPLE B
Preparation of H
HZN ~ N C02Et O / ( OH HCl CI '" Br St6D 11 s Preparation of cl To a suspension of 3-bromo-5-chlorosalicy(aldehyde (175.0 g, io 743.2 mmol) in acetic anhydride (280.5 mL, 3.0 mol) was added triethylamine (103.6 mL, 743.2 mmol). The reaction solution was heated at reflux for 4.5 hours. The solution was cooled and concentrated in vacuo.
To the brown residue was added absolute ethanol (730 mL). The mixture was stored at 0°C for 14 hours. The brown solid was collected by filtration is and washed with cold ethanol. The solid was dried in vacuo to give the desired product {123.0 g, 64% yield). 'H NMR was consistent vfrith the proposed structure.
to 2 2o Preparation of HCI
CI or To a suspension of the coumarin (40:0 g, 154.1 mmol) in THF (400 mL) at -76°C was added, dropwise with stirring, lithium bis(trimethylsilyl)-amide {154.1 mL of a 1 M solution in THF). The addition was completed in minutes. The reaction mixture was then stirred for 5 minutes, warmed s to -20°C and stirred for 15 minutes. To this solution was added acetic acid (9.25 g, 154.1 mmol) in THF (28 mL) over 5 minutes. The mixture was warmed to room temperature and volatiies were removed in vacuo. The residue was dissolved in ether (850 mL), washed with saturated aqueous NaHC03 (2 x 100 mL), brine (2 x 40 mL) and dried (MgS04). The ether io solution was concentrated to about 160 mL and cooled to 0 °C. To this suspension was added 4M HCI in dioxane (56.3 mL, 225 mmol) and the mixture was stirred at 0°C for 30 minutes. The suspension was filtered and the filter cake washed with ether. The solid was dried in vacuo to give the desired product as the HCI salt, dioxane solvate, (45.0 g). 'H NMR was is consistent with the proposed structure.
to 3 Preparation of HZN CpZEt OH
HCI
Br CI
To a suspension of the lactone {142.2 g, 354.5 mmol) in absolute ethanol (533 mL) was added 4M HCI in dioxane {157.8 mL, 631.1 mmol) over 10 minutes. The reaction mixture was stirred at room temperature for 2.5 hours. Volatiles were removed in vacuo. The residue was dissolved in 2s ethyl acetate (450 mL) and the solution kept at 0°C for 15 hours.
The tan precipitate was collected by filtration and washed with cold ethyl acetate.
The solid was dried in vacuo to give the desired product as the hydrochloride salt (100.4 g, 79% yield). 'H NMR was consistent with the proposed structure.

Step 4 Preparation of O
N
O H ~' COZEt O / OH
CI er s To a flame-dried round bottom flask (0.1 L) equipped with magnetic stir bar was added N-t-Boc-glycine N-hydroxysuccinimide ester (Sigma, 2.72 g, 0.010 mol), dry THF (Aldrich Sure Seal, 50 mL) and the product from Step 3 (3.10 g, 0.01 mole, vacuum desiccated overnight over P205) under an inert atmosphere (Ar). The reaction mixture was cooled to 1o approximately 0°C (salt-ice bath) and triethylamine (1.01 g, 0.010 mole) was added. The reaction was ~aliowed to proceed overnight. The reaction mixture was concentrated to a semi-solid and worked up in a fashion similar to Example A, Step 3. Volatiies were removed from the organic layer under vacuum at 55°C to give an oil (4 g, 83% yield) that solidified on 1s standing.
MS and NMR were consistent with the desired structure.
Stec 5 Preparation of H
HZN ~'' N C02Et O , I OH HCl 2o CI ~ Br The product obtained in Step 4 was de-protected to give the amine hydrochloride salt using the following procedure. To the product from Step 4 (4.0 g, 0.0084 mole) in a flame-dried round bottom flask (0.1 L) with stir bar was added dry dioxane (20 mL). To this was added 4.0 N HCI in 2s dioxane (20 mL) and the reaction allowed to proceed until gas evolution ceased and the reaction was complete (about one hour). Volatiles were removed under vacuum and the residue triturated with diethyl ether (50 mL). Solids were collected by filtration and washed with ether and dried to give a tight broom solid {2.7 g, 78°r6 yield).
s MS and NMR were consistent with the desired structure.

EXAMPLE C
Preparation of HZN ~ COZEt o , I OH HCl 8r ' Br s Step 1 I
e~ ' er To a suspension of 3,5-dibromosalicylaldehyde (100 g, 357 mmol) to in acetic anhydride (164.8 mL, 1.8 mol) was added triethyiamine (45 mL, 375 mmol). The reaction solution was heated overnight at reflux under argon. The solution was cooled to room temperature and a solid mass formed. The dark brown reaction mixture was washed with hot hexanes (3 x 300 mL) and aqueous saturated sodium bicarbonate. The resulting ~s solid was dissolved in EtOAc (2L) and washed with water. The organic layer was dried (sodium sulfate} and concentrated to give a brown solid that was collected by filtration. ~ The solid was dried in vacuo to give substantially pure 6,8-dibromocoumarin (94.2 g, 8?°~ yield).
MS and'H NMR were consistent with the desired structure.

Steo 2 HZN

o ~HC~'~
I o Br ~' Br To 6,8-dibromocoumariri (20.0 g, 0.066 mo!) (prepared in Step 1 ) in s THF (100 mL} at -78°C was added dropwise with stirring lithium bis(trimethylsilyl}amide (66 mL of a 1 M solution in THF). The addition was completed in 10 minutes. The reaction mixture was then stirred for 5 minutes, warned to 0°C and stirred for 15 minutes. To this solution was added acetic acid (3.95 g) over one minute. The mixture was warmed to io room temperature and volatiles were removed in vacuo. The residue was dissolved in hexanes (500 mL}, washed with saturated aqueous NaHCO3 (2 x 100 mL) and dried (Na2S0,,). The organic solution was concentrated to give an oil that was immediately taken up in diethyl ether (400 mL) and 4M HCI in dioxane (30 mL) was added with stirring at 0°C for 30 minutes.
is Excess HCI was removed in vacuo, the suspension filtered and the filter cake washed with ether. The solid was dried in vacuo to give the desired product as the HCI salt, dioxane solvate (19.9 g).
MS and'H NMR were consistent with the desired structure.
~u to 3 H2N COZEt OH
~ HC1 Br ~ Br The (actone prepared in Step 2 above (15 g) was dissolved in absolute ethanol (400 mL) and anhydrous HCI gas was passed through for 2s one minute. The reaction mixture was stirred at room temperature for 2.5 hours. RPHPLC showed complete reaction. The volatiles were removed in vacuo to give a dark residue. The residue was triturated with diethyl WO 99/44994 PC1'IUS99103281 ether (500 mL) and the mixture stirred overnight. The tan precipitate was collected by filtration and washed with diethyl ether. The solid was dried in vecuo to give the desired product as the hydrochloride salt (15.2 g).
MS and'H NMR were consistent with the desired structure.
~teo 4 O
'~I~ ,! H
~O~ N~ N C Et H
O / OH
Br ~ Br To a flame-dried round bottom flask (0.2 L) equipped with magnetic 1o stir bar was added N-t-Boc-glycine N-hydroxysuccinimide ester (Sigma, 8.1 g, 0.030 mol), dry DMF (Aldrich Sure Seal, 50 mL) and the product of Step 3 (12 g, 0.03 mole, vacuum desiccated overnight over P20s) under an inert atmosphere (Ar). The reaction mixture was cooled to approximately 0°C (salt-ice bath) and N-methyl morpholine (3.03 g, 0.030 mole) and 1s catalytic DMAP added. The reaction was allowed to proceed overnight warming to room temperature. The reaction mixture was concentrated to a semi-solid and worked up in a fashion similar to Example A, Step 3.
Volatiles were removed from the organic layer under vacuum at 55°C
to give an oil (15.7 g, 93% yield) that solidified on standing. .
2o MS and NMR were consistent with the desired structure.

WO 99144994 PCTNS99/032$1 to 5 H
H2N ~ N C02Et O , / ' OH HCl Br 8r The product obtained in Step 4 was deprotected to give the amine s hydrochloride salt using the following procedure. To the product from Step 4 (13.0 g, 0.00134 mole) in a flame-dried round bottom flask (0.1 L) with stir bar was added dry dioxane (40 mL). To this was added 4.0 N HCI in dioxane (30 mL) and the reaction allowed to proceed until gas evolution ceased and the reaction was complete (about one hour). The volatiles 1o were removed under vacuum and the residue triturated with diethyl ether {50 mL). Solids were collected by filtration and washed with ether and dried to give a solid (10.6 g, 93°~ yield).
MS and NMR were consistent with the desired structure.

EXAMPLE D
Preparation of H
HZN ~' N COZEt O / ~ OH , HCl 8r ~ CI
Step 1 Preparation of 3-chloro-5-bromosalicylaldehyde H O
OH
Br ~' CI
To a 5L round bottom flask fitted with a mechanical stirrer and gas addition tube was added 5-bromosalicylaldehyde (495 g, 2.46 mot) and to acetic acid at ambient temperature to form a slurry. To this mixture was added chlorine gas at a moderate rate until a slight molar excess of chlorine (183 g, 1.05 mol) had dissolved. After the addition was stopped the reaction allowed to proceed overnight. The solid formed was recovered by filtration and the filtrate diluted into water (2.5L). The mixture is was stirred vigorously for 20 minutes, the product collected by filtration and washed with water. The combined solids were vacuum dried to give the desired 3-chloro-5-bromosalicylaldehyde (475 g, 82°~ yield).
MS and'H NMR were consistent with the desired structure.
20 to 2 Preparation of 6-bromo-8-chlorocoumarin O

~I
Br cl WO 99/44994 PCT/US99/032$1 To a 5L round bottom flask fitted with a mechanical stirrer and condenser was placed 3-chioro-5-bromosalicylaldehyde (554.1 g, 2.35 mol, 1 equiv.), acetic anhydride (1203g , 11.8 mol, 5 equiv.) and triethylamine (237.4 g , 2.35 mol, 1 equiv.). The reaction solution was s heated at reflux (131-141°C) overnight. The dark brown reaction mixture was cooled to 50°C and ice (2 L) added (ice-bath cooling) with stirring.
After one hour the mixture was filtered and the filtrate combined with EtOH
(1 L). To this mixture was added EtOH (300 mL) and the reaction mixture stirred for one hour. The precipitate that formed was cvliected by filtration ~o and washed with water : EtOH (3 x 1.3 L), vacuum and dried then dried on a fluid-bed drier. The total isolated yield is 563 g or 92°~.
MS and'H NMR were consistent with the desired structure.
Step 3 ~s Preparation of 3-amino-3- (2-hydroxy-3-chioro-5-bromo)phenyl propanoic acid ethyl ester H2N C02Et OH ~ HCI
Br ~ C1 To a 3-neck 5L round bottom flask fitted with a mechanical stirrer 2o was added 6-bromo-8-chlorocoumarin (300 g, 1.16 mol) (prepared in Step 2) and dry THF (900 mL, Aldrich Sure Seal). The resulting mixture was cooled to less than -45°C (dry icelacetone bath) and lithium bis (trimethylsilyl)amide (0.80 rnol , 800 mL of 1 M in THF and 0.6 L in hexanes, 1.2 equivalents) added while maintaining temperature below 2s ~ -45°C for 0.5 hour. In a separate 5L flask EtOH (2.5 L) and HCI (4 N HCI
in dioxane, 1 L) were combined at -15°C. The coumarin reaction was quenched by addition of the cooled HCI I EtOH solution. After 0.5 hour the resulting reaction mixture temperature was -8.3°C. The reaction mixture was kept at 0°C overnight, concentrated to about 2.5 L and partitioned w0 99/44994 PCT/US99/03281 between EtOAc (3 L) and water (4 L}. The organic layer was washed with aqueous HCI (4 x 1.2 L, 0.5 N HCI). The pH of the combined aqueous layers was adjusted to about 8 by addition of 10°.6 aqueous NaOH and extracted with methylene chloride (1x7 L and 3 x 2L). The combined s organic layers were dried (MgSO,,, 900 g}, filtered, and 4M HCI in dioxane (400 mL) added with stirring. Upon completion of precipitation the solid was removed by filtration. The mixture was concentrated to 2.5 L, hexanes added (2.5 L) and the precipitate isolated by filtration. The filter cake was washed with methylene chloridelhexanes (1 : 2), suction dried and vacuum to oven dried at 40°C to obtain the desired product (257 g, fi0%
yield).
MS and'H NMR were consistent with the desired structure.
Step 5 is Preparation of O
H
~O~ N N C02Et H~ OH
i Br ~ CI
The above compound was prepared using essentially the same procedure and relative quantities as specified for its isomer in Example B, Step 4.
2o MS and'H NMR were consistent with the desired structure.
Steh 6 Preparation of H
H2N ~ N C02Et O ~,, ' OH -~ HCI
2s Br \ CI

VlrO 99144994 PCT/US99/03281 This compound was prepared using essentially the same procedure and relative quantities as specified for its isomer in Example B, Step 5.
MS and'H NMR were consistent with the desired structure.

EXAMPLE E
Preparation of H
HZN ~ N C02Et O / I OH , HCI
CI ~ I
Step 1 Preparation of 3-iodo-5-chiorosalicylaldehyde.
H O
OH
CI ~ 1 N-lodosuccinimide (144.0 g, 0.641 mole) was added to a solution of io 5-chlorosaficylaldehyde (100 g, O.fi38 mole) in dimethylformamide (400 mL). The reaction mixture was stirred for 2 days at room temperature.
Additional N-iodosuccinimide (20.0 g) was added and the stirring was continued for an additional 2 days. The reaction mixture was diluted with ethyl acetate (1 L), washed with hydrochloric acid (300 mL, 0.1 N), water is (300 mL), sodium thiosulfate (5%, 300 mL), brine (300 mL), dried (MgSO,) and was concentrated to dryness to afford the desired aldehyde (162 g, 90°~ yield) as a pale yellow solid.
MS and NMR were consistent with the desired structure.
2o to 2 Preparation of 6-chloro-8-iodocoumarin O
O
CI ~ I

A mixture of 3-iodo-5-chlorosalicylaldehyde (100_g, 0.354 mole), acetic anhydride (300 mL) and triethylamine (54 mL) was heated at reflux for 18 hours. Upon cooling, the desired coumarin precipitated as a dark brown crystalline material. This was filtered, washed with hexanelethyl s acetate (4:1, 200 mL); and was air dried. Yield: 60 g (55°~).
MS and'H NMR were consistent with the desired structure.
Preparation of (R,S) -4-amino-3,4-dihydro-6-chloro-8-iodocoumarin to hydrochloride.

O ~ HCI
CI ~ I
Lithium hexamethyldisilazane (21.62 mL, 1 M, 21.62 mmol) was added to a solution of 6-chloro-8-iodocoumarin (6.63 g, 21.62 mmol) in tetrahydrofuran (100 mL) at -78°C. The reaction mixture was stirred at this is temperature for 30 minutes, then at 0°C for 1 hour. Acetic acid (1.3 g, 21.62 mmol) was added to the reaction mixture. The reaction mixture was poured into ethyl acetate (300 mL) and saturated sodium carbonate (200 mL) solution. The organic layer was separated, washed with brine (200 . mL), dried (MgSO,,), and was concentrated to afford a residue. The 2u residue was added-to anhydrous ether (200 mL) followed by dioxanelHCl (4N, 30 mL) at 0°C. The reaction mixture was stirred for 1 hour at room temperature, filtered, and was dried in vacuo to afford the desired product (4.6 g, 59°~ yield) as a powder. (RPHPLC: Rf 6.8 minutes; Gradient 10°~
acetonitrile -90°~ acetonitrile over 15 minutes then to 100°~
acetonitrile 2s over the next 6 minutes. Both water and acetonitrile contain 0.1 °~
TFA.
Vydac C18 protein peptide column, 2 mU minutes flow rate, monitored at 254 nm).
MS and'H NMR were consistent with the desired structure.

WO 99/44994 PC'T/US99/03281 to 4 Preparation of (R, S)-Ethyl 3-amino-3-(5-chloro-2-hydroxy-3-iodo)phenyl propionate hydrochloride.
H2N C02Et OH ~ HCI
C! ~ I
s Hydrogen chloride gas was bubbled into a solution of 4-amino-3,4-dihydro-6-chloro-8-iodocoumarin hydrochloride (22.0 g, 61.09 mmol) in ethanol (250 mL) keeping the reaction mixture at 0-10°C till saturation.
After 6 hours at reffux, most of the solvent was removed by distillation.
The cooled residue was added to anhydrous ether and was stirred for 2 1o hours. The initial gum turned into a crystalline material. The crystalline product was filtered and was dried to afford the desired product (20 g, 81 %
yield) as a off-white crystalline powder. (Rf 7.52 minutes, conditions as Step 3).
MS and'H NMR were. consistent with the desired structure.
is to 5 Preparation of (R,S)-ethyl 3-(N-BOC-gly)-amino-3-(5-chloro-2-hydroxy-3-iodo)phenyl propionate.
O H
~O~N~I,I.. N COZEt H O OH
CI ~ I
A mixture of BOC-gly (2.16 g, 12.31 mmol), HOST (1.67 g, 12.31 yield), EDCI (2.36 g, 12.31 mmol) and OMF (50 mL) was stirred at 0°C
for 1 hour. Ethyl 3-amino-3-(5-chloro-2-hydroxy-3-iodo)propionate hydrochloride (5.0 g, 12.31 mmol) was added to the reaction mixture 2s followed by triethylamine (3.5 mL). The reaction mixture was stirred for 18 hours at room temperature. DMF was removed in vacuo and the residue was partitioned between ethyl acetate (300 mL) and sodium bicarbonate (200 mL). The organic layer was washed with hydrochloric acid (1 N, 100 mL), brine (200 mL), dried (MgS04) and was concentrated to afford the desired product as a solid (fi g, 93°r6 yield).
s MS and'H NMR were consistent with the desired structure.
sceo ss Preparation of (R,S)-ethyl 3-(N-gly)-amino-3-(5-chioro-2-hydroxy-3-iodo)phenyl propionate hydrochloride.
H
H2N ~ N C02Et O / ( OH , IiCI
C1 ~' I
DioxaneIHCI (4N, 20 mL) was added to ethyl 3-(N-BOC-gly)-amino-3-(5-chloro-2-hydroxy-3-iodo)propionate (fi.0 g, 11.39 mmol) at 0°C and was stirred at room temperature for 3 hours. The reaction mixture was is concentrated, and concentrated once more after addition of totuene (100 mL). The residue obtained was suspended in ether and was filtered and dried to afford the desired product as a crystalline powder (5.0 g, 95°~
yield). (RPHPLC : Rf 8.3 minutes, conditions as in Step 3).
MS and'H NMR were consistent with the desired structure.

EXAMPLE F
Preparation of H
HEN ~ N COZEi O / OH , HCI
\l er I
to 1 s Preparation of 3-iodo-5-bromosalicylaldehyde To a solution of 5-bromosalicylaldehyde (20.0 g, 0.1 mole) end potassium iodide (17 g , 0.1 mole) in acetonitrile (150 mL) and water (50 mL) in a 500 mL round bottom flask with magnetic stirrer was added io chloramine T (23 g, 0.1 mole). The mixture was allowed to react for one hour. The reaction mixture was partitioned between hydrochloric acid (10%, 200 mL) and ethyl acetate. The organic layer was dried (Na2S0~), filtered and concentrated in vacuo. To the residue was added hexanes and the reaction mixture heated to 50°C for 15 minutes. The undissolved is material was removed by filtration. The ~Itrate was concentrated in vacuo to. leave canary yellow 3-iodo-5-bromosaiicylaldehyde (26 g).
MS and'H NMR were consistent with the desired structure.
to H
HyN'~ N C02Et O ~, ( OH . HCI
20 Br \ I
The above compound was prepared using essentially the same procedure of Example E, Steps 2-6 where in Step 2, an equivalent amount of product from Step 1, 3-iodo-5-bromo-salicylaldehyde, was substituted 2s for 3-iodo-5-chlorosaiicylaldehyde.
MS and'H NMR were consistent with the desired structure.

W.O 99144994 PCTIUS99/03281 EXAMPLE H
Preparation of H H O
N'~ N ~, I OH
N ~ ~ HCI
HO
OH
Step 1 s Ethanol (375 mL) and deionized water (375 mL) were added to a 2L
3-neck round bottom flask fitted with a mechanical stirrer, Cfaisen adapter, addition funnel, reflux condenser and thermocouple. 1,3- diamino-2-hydroxypropane (125.04 g, 1.39 mol) (Aldrich) was added to the reaction io flask and stirred to dissolve. Carbon disulfide (84 mL , 1.39 mol) was added in a drop-wise fashion via addition funnel at 25-33°C over a 35 minute period to afford a milky-white mixture. The temperature was maintained with an ice bath. The reaction mixture was refluxed at 73.4°C
for two hours to afford a yellow solution. The reaction mixture was cooled is with an ice bath to 25°C and concentrated HCI (84 mL) was added in drop-wise fashion while maintaining the temperature at 25-26°C. The reaction mixture was refluxed for 21 hours at 78.4°C. The reaction solution was doled to 2°C and product collected via vacuum filtration. The white solid was washed 3 times with ice bath chilled ethanol : water (1:1 ) (50 mL) and 2o dried in vacuo at 40°C to afford 5-hydroxytetrahydropyrimidine-2-thione (63.75 g, 34.7°~ yield) as a white solid.
MS and NMR were consistent with the desired structure.
t 2 2s 5-Hydroxytetrahydropyrimidine-2-thione (95 g, 0.72 mol) prepared in Step 1, absolute ethanol (570 mL), and methyl iodide (45 mL , 0.72 rnol) were added to a 2 L round bottom flask fitted with a mechanical strirrer and thermocouple. The reaction mixture was refluxed at 78°C for 5 hours and then cooled to room temperature. The reaction mixture was concentrated V1!O 99144994 PCT/US99103281 in vacuo to afford a white solid (194.72 g). The white solid was triturated 3 times with ethyl ether (500 mL ) and dried in vacuo to afford 2-methylthioether-5-hydroxypryrimidine hydroiodide (188.22 g, 95.4% yield) as a white solid.
s MS and'H NMR were consistent with the desired structure.
Steo 33 2-Methyl thioether-5-hydroxypyrimidine hydroiodide (150.818, 0.55 mol), methylene chloride (530 mL), dimethylacetamide (53 mL) and triethylamine (76.7 mL, 0.55 mol) were added to a 2L 3-neck round bottom flask fitted with reflux condenser, mechanical stirrer and a static atmosphere of nitrogen. The mixture was cooled with an ice bath and di-tert -butyl Bicarbonate (120.12 g, 0.55 mol) was added at 4°C. The 1s reaction mixture was heated at 42.5°C for 18 hours to afford a light yellow . solution. The reaction solution was transferred to a 2L separatory funnel and washed 3 times with DI water (200 mL), dried with MgSOs, filtered and concentrated in vacuo to afford 8oo-2-methylthioether-5-hydroxypyrimidine (134.6 g, 99.35% yield) as a light yellow viscous oil.
2o MS and'H NMR were consistent with the desired structure.
Stea 44 Boc-2-methylthioether-5-hydroxypyrimidine (50.3 g, 0.204 mol), 3-2s amino-5-hydroxybenzoic acid (Aust. J. Chem. (1981 ) 34(6), 1319 24) (25.0 g, 0.1625 mole) and 50 mL anhydrous DMA were heated at 100°C with stirring for 2 days. A slurry precipitate resulted. The reaction was cooled to room temperature and the precipitate was filtered, washed with CH~CN, then ethyl ether and dried. This solid was slur~ied in H20 and acidified 3o with concentrated HCI resulting in a solution. This was frozen and lyophilized to yield the desired product as a white solid (14.4 g).
MS and'H NMR were consistent with the desired structure.

EXAMPLE I
Preparation of C OzEt OH
~ HCI
CI
(S isomer) Step 1 s Preparation of Reformatsky Reagent ~ COz-t-Bu Br-Zn A 4-L flask fitted with a condenser, temperature probe and ~o mechanical stirrer was charged with Zn metal (180.0 g, 2.76 mol, -30 -100 mesh) and THF (1.25 L). While stirring, 1,2-dibromoethane (4.74 mL, 0.05 mol) was added via syringe (alternatively, TMS CI (0.1 equivalent) at room temperature for one hour can be substitutedj. After inert gas purge (3 N~/vacuum cycles) the suspension of zinc in THF was heated to reflux is (65°C} and maintained at this temperature for 1 hour. The mixture was cooled to 50°C before charging tert-butyl bromoacetate (488 g, 369 mL, 2.5 mol) via 50 mL syringe and syringe pump (delivery set to 4.1 mUminutes} over 1.5 hours. Reaction temperature of 50° +I- 5°C
was maintained throughout the addition. The reaction mixture was allowed to 2o stir at 50°C for one hour after the addition was complete.
Subsequently, the mixture was allowed to cool to 25°C and the precipitated product allowed to settle. The THF mother liquor was decanted into a 2-L round 2s bottom flask using a coarse fritted filter stick and partial vacuum transfer (20 mm Hg). This removed about 65% of the THF from the mixture. 1-Methyl-2-pyrrolidinone (NMP, 800 mL) was added and agitation resumed for 5 minutes. The reaction mixture can be filtered to remove any remaining zinc. Analysis indicated a titer of desired Refonnatsky reagent SO

of 1.57 M with a molar yield of 94°~. Alternatively, the solid reagent can be isolated by filtration from the original reaction mixture. The cake can be washed with THF until a white solid is obtained and dried under N~ to obtain the desired product as a mono THF solvate that may be stored at -s 20°C (desiccated) for extended periods. Typical recoveries are 85-90°~.
2A. Preparation of x o i o~o../~oi t CI ~ CI
Potassium carbonate (powder, oven dried at 100°C under vacuum, 8.82 g, 60 mmoles) was added to a solution of 3,5-dichlorosalicylaldehyde (11.46 g, 60 moles) in DMF (40 mL) at room temperature to give a bright Is yellow slung. MEMCI (neat, 7.64 g, 61 mmoles) was then added while maintaining the bath temperature at 20°C. The mixture was then stirred at 22°C for 6 hours and MEMCI (0.3 g, 2.4 mmoles) was added. The mixture was stirred for another 0.5 hour and the reaction mixture poured into cold water (200 ml) to precipitate the product. The slurry was filtered on a pressure filter and the cake was washed with water (2 x 50mL) and was dried under NZlvacuum to afford the product (14.948, 89°~) as a off white solid. 'H NMR (CDC13, TMS) 3.37 (s, 3H), 3.54 to 3.56 (m, 2H), 3.91 to 3.93 (m, 2H), 5.30 (s, 2H), 7.63 (d, 1 H), 7.73 (d, 1 H), 10.30 (s, 1 H); "C
NMR (CDC13, TMS) d (ppm):59.03, 70,11, 99.57, 126.60, 129.57, 130.81, 2s 132.07, 135.36, 154.66, 188.30. DSC: 48.24°C (endo 90.51J1g);~
Microanalytical: calcd for C"H,ZCI20,: C: 47.33%; H: 4.33%; CI: 25.40%;
found: C: 47.15°~; H:4.26°~; CI: 25.16%.
sI

2B. Preparation of ~o~
m The product from Step 2A (35.0 g, 0.125 mol) was charged in a 1-L
s 3-neck round bottom flask fitted with a mechanical stirrer and an addition funnel followed by addition of THF (200 mL).The solution was stirred at 22°C and (S)-phenylglycinol (17.20 g, 0.125 mol) was then added at once.
After 30 minutes at 22°C, MgSO, (20 g) was added. The mixture was stirred for 1 hour at 22°C, and filtered on a coarse fritted filter.
The filtrate 1o was concentrated under reduced pressure. No further purification was performed and the crude imine was used directly in the coupling reaction, Step 2, C.
is 2C. Preparation of OH
O ~
O O O
/ ~ ~ ~O/
CI ~ CI
A 1-L 3-neck round bottom flask fitted with a mechanical stirrer and an addition funnel was charged with the solid reagent produced in Step 1 20 (91.3 g, 0.275 mol) and NMP (200 mL) under nitrogen. The solution was then tooted to -10°C and stirred at 350 rpm. A solution of imine (prepared in Step 2B) in NMP was prepared under nitrogen and then added over 20 minutes to the above reaction mixture while the temperature was maintained at -5°C (jacket temperature -10°C). The mixture was stirred for an additional 1.5 hours at -8°C and one hour at -5°C after the addition was complete., After cooling to -10°C a mixture of concentrated HCllsaturated s solution of NH,CI (8.1 mU 200 mL) was added in 10 minutes. MTBE (200 ml) was added end the mixture was stirred 15 minutes at 23°C at 200 rpm.
Stirring was stopped and the layers separated. The aqueous layer was extracted with MTBE (100 mL). The two organic layers were combined, washed successively with a saturated solution of NH,,CI (100 mL), water (100 mL) and brine (100 mL). The solution was dried with MgSO, (30 g), filtered and concentrated to afford an orange oil (66.3 g) (soiid~es in standing) containing the desired product as a single diastereoisomer (confirmed by proton and carbon nmr). A sample was purified for analysis by recrystallization from heptane to afford the product as an off-white solid.
is Proton and carbon NMR and IR spectra were consistent with the desired structure. [aj°n = + 8.7° (c = 1.057, MeOH).
Microanalytical:
calcd for CisH~CIzNOs:
C: 58.77%; H: 6.47%; N: 2.?2%; Cl: 13.78%
found: C: 58.22%; H: 6.54%; N: 2.70°h; C1:13.66°~.
Preparation of HZN COyEt S03H
OH
CI ~ CI CHa 2s 3A. A solution of the crude ester prepared in Step 2 [17.40 g, 0.033 mole (theory)], and EtOH (250 mL) was charged to a 1-L 3-neck jacketed reactor. The solution was cooled to 0°C and Pb (OAc)4 ( 14.63 g, 0.033 mole) was added at once. After 2 hours a 15% solution of NaOH (30 mL) was added and ethanol was removed under reduced pressure. Another portion of 15°~ NaOH (100 mL) was added and the mixture extracted with MTBE (2 x 100 mL), washed with Hz0 (2 x 100 mL) and brine (50 mL), dried with NazSO,, filtered on celite and concentrated under reduced s pressure to afford an.orange oil (12.46 g). The oil was homogeneous by thin layer chromatography (tlc) and was used without further purification.
3B. The oil from 3A was diluted with EtOH (30 mL) and paratoluene sulfonic acid {1.3 equiv., 0.043 mole, 8.18 g) was added. The solution 1o was heated to reflux for 8 hours, cooled to ambient temperature and concentrated under reduced.pressure. The residue was treated with THF
(20 mL) and heated to reflux to form a solution. The solution was cooled to room temperature and the compound crystallized. Heptane (30 mL) and THF (10 mL) were added to form a fluid slurry which was filtered. The 1s cake was washed with THFlheptane (40 mL, 1l1 ) and vacuum dried for two hours in a pressure filter under nitrogen to afford a white solid (7.40 g).
Proton and carbon NMR and IR spectra were consistent with the desired product as substantially a single enantiomer.
Microanalytical: calcd for C,8H2,CIzNOsS, 0.25 C,HsO:
20 C: 48.73%; H: 4.95%; N: 2.99%; CI: 15.14%
found: C: 48.91 %; H: 4.95%; N: 2.90%; C1:14.95%.
to 4 2s Preparation of COzEt C
OH
CI ~ CI

To a 500 mL round bottom flask equipped with a magnetic stir bar and nitrogen bubbler were charged the free base of the product produced in Step 3 (21.7 g , 0.065 mole), N-t-Boc-glycine N-hydroxysuccinimide ester (17.7 g, 0.065 mole) and DMF (200 mL). The reaction mixture was s stirnad under nitrogen at room temperature for 3.25 hours and a pale orange solution formed. The reaction mixture was poured into ice-cold ethyl acetate (1.2 L). The organic solution was washed with 1 M HCI (250 mL) and then with brine (500 mL), dried (MgSO,) and concentrated under vacuum to near dryness to obtain an oil that was subsequently dried at io 50°C to obtain the product as a colorless oil (28.12 g , 99 °~). Seed crystals were prepared from ethyl acetatelhexanes. The product (about 28 g) was dissolved in ethyl acetate (35 mL) and hexanes (125 mL). The solution was seeded with the seed crystals and precipitate formed. The solids were filtered and dried overnight under vacuum at 55°C to yield a is colorless solid (27.0 g, 95°~6).
MS and'H NMR were consistent with the desired structure.
~teP 5 Preparation of C02Et HZN ~ N
~ HCI
Cl ~' CI
The Boc-protected glycine amide prepared in Step 4 (27.0 g, 0.062 2s mole) was dried overnight over P20s and NaOH pellets. The solid was dissolved in dioxane (40 mL) and the solution cooled to 0°C. An equivalent volume of 4N HCUdioxane (0.062 mole} was added and the reaction was run for 2 hours. At this point the conversion was 80% by RPHPLC. The reaction mixture was allowed to warm to room temperature 5s VSO 99144994 PC"T/US99I03281 over 4 hours. The reaction mixture was concentrated at 40°C to a foam which was triturated with ether (200 mL). The white solid that formed was filtered and dried over P205 to yield the desired glycine beta-amino acid ethyl ester compound, as an HCI salt (20.48, 88.5°~6 isolated yield).
s MS and'H NMR were consistent with the desired structure.

Vk0 99/44994 PCT/US99103281 EXAMPLE J
Preparation of H C02Et N

O OH ~ HCl CI ~ Br to 1 Preparation of MEM protected 3-bromo-5-chlorosalicylaldehyde (129.42 g, 0.4 io mol), prepared according to the procedure of Example I, Step 2A. An equivalent amount of 3-bromo-5-chlorosalicylaldehyde was substituted for 3,5-dichlorosaticylaldehyde, which was charged in a 2-L 3-neck round bottom flask fitted with a mechanical stirrer, followed by addition of THF
(640 ml) and (S)-phenytglycinol (54.86 g, 0.4 mol). After 30 minutes at is 22°C, MgSO~ (80 g) was added. The mixture was stirred for 2 hours at 22°C, and filtered on a coarse fritted filter. The filtrate was concentrated under reduced pressure to afford a pale yellow oil (180.0 g) containing the desired imine. No further purification was performed and the crude product was used directly in the coupling reaction, Step 2.
2o Microanalytical: calcd for C,9HI,BrCINO.:
C: 51.54%; H: 4.78%; N: 3.16%; Br: 18.04%; CI: 8.00%
found: C: 50.22°~; H: 4.94%; N: 2.93%; Br: 17.15%; C1:7.56%.

WO 99/44994 PC'T/US99/03281 to 2 Preparation of C02t-Bu HO . i I O~O~Oi CI ~ Br s In a 5-L 3-neck round bottom flask fitted with a mechanical stirrer, the reagent from Example I, Step 1 (332.0 g, 0.8 mol) was taken up in NMP (660 mL) under nitrogen. The solution was then cooled to -10°C. A
solution of imine from Step 1 in NMP (320 ml) was prepared under nitrogen io and then added over 30 minutes to the above reaction mixture while the temperature was maintained at -5°C. The mixture was stirred for an additional hour at -8°C and at -5°C for 2 hours after addition was complete and then cooled to -10°C. A mixture of concentrated HCl/saturated solution of NH,CI (30mLl720 rnL) was added over 10 minutes. MTBE (760 is ml) was added and the mixture was stirred for 30 minutes at 23°C.
Stirring was stopped and the layers separated. The aqueous layer was extracted with MT8E (320 ml). The organic layers were combined, washed successively with saturated aqueous NH,CI (320 ml), DI water (320 mI) and brine (320 ml). The solution was dried with MgSO, (60 g), filtered and 2o concentrated to afford a yellow oil (221:0 g) containing the desired product as a single diastereoisomer as determined by proton NMR.
DSC: 211.80°C (endo. 72.56 J/g), 228.34°C (98.23 J/g);
Microanalytical: calcd for C~H33BrCINOs:
C: 53.72%; H: 5.95%; N: 2.50%; Br: 14.29%; CI: 6.33%
2s found: C: 52.11 %; H: 6.09%; N: 2.34 %; Br: 12.84%; C1:6.33%.

to Preparation of ~N C4zEt S~H
OH .
CI 'Br A solution of crude ester, prepared in Step 2 (~? 11 g), in ethanol s (1500 mL) was charged under argon atmosphere to a 3-L 3-neck round bottom flask frtted with a mechanical stirrer. The reaction mixture was cooled to 0°C and lead tetraacetate (88.fi7 g, 0.2 mot) was added in one portion. The reaction mixture was stirred for 3 hours at 0°C and then 15%
aqueous NaOH (150 mL) was added to the reaction mixture below 5°C.
1o Methanol was removed under reduced pressure on rotavap. Another 150 mL of 15°~ aqueous NaOH was added and the reaction mixture was extracted with ethyl acetate (3 x 300 mL) and washed with DI water (2 x 100 mL) and brine (2 x 100 mL) and dried aver anhydrous MgSO~ (30 g).
It was then filtered over celite and concentrated under reduced pressure to 1s give the desired product (103 g) as a red oil.
to 4 Preparation of H COzEt H2N ~ N
O OH ° ~iCl ~i C~ ~ Br 20 The above compound was prepared according to the procedure employed for Example I, Step 4 and Step 5 by substituting an equivalent amount of the product from Step 3 in Example I, Step 4. MS and'H NMR
were consistent with the desired structure.

WO 99/44994 PC'TIUS99/03281 EXAMPLE K
Alternate Preparation of the compound of Example J
to Preparation of i w ~ O
s C
To the product of Example B, Step 3, (50.0 g, 139.2 mmol) and NaHCOa (33.5 g, 398.3 mmol) was added CHZCIz (500 mL) and water (335 mL). The mixture was stirred at room temperature for 10 minutes. A
solution of benzyl chloroformate (38.0 g, 222.8 mmol) in CH=CIZ (380 mL) to was added over 20 minutes with rapid stirring. After 50 minutes, the reaction mixture was poured into a separatory funnel and the organic Layer collected. The aqueous phase was washed with CHZCIZ (170 mL). The combined organic layers were dried (MgSO,) and concentrated in vacuo.
The resulting gummy solid was triturated with hexane and collected by is filtration. The tan solid was dried in vacuo to give the desired racemic product (61.2 g, 9fi°~ yield). This material was subjected to reverse phase HPLC using a chiral column to give each pure enantiomer. The column employed was a Whelk-0 (R,R), 10 micron particle size using a 90 : 10 heptane : ethanol mobile phase. Optical purity was determined to be >
20 98°~ using analytical hplc using similar column and solvent conditions. 'H
NMR was consistent with the proposed structure.
to 2 Et w To a solution of the compound obtained in Step 1. (48.5 g, 106.2 mmol) in CH=CI2 (450 mL) was added trimethylsilyl iodide (25.5 g, 127.4 mmol) in GH=CI=(100 mL) via canula. The orange solution was stirred at room temperature for 1 hour. Methanol (20.6 mL, 509.7 mmol) was added s dropwise and the solution stirred for 15 minutes. The reaction solution was concentrated in vacuo to give an orange oil. The residue was dissolved in methyl t-butyl ether (500 mL) and extracted with 1 N HCI (318 mL) and water (1 x 200mL, 1 x 100 mL). The aqueous extracts were back washed with MTBE (100 mL). To the aqueous solution was added solid 1o NaHCO, (40.1 g, 478 mmol) in small portions. The basified aqueous mixture was extracted with MTBE (1 X 1 L, 2 X 200 mL). The combined organic solution was washed with brine and concentrated in vacuo to give the desired product (23.3 g, 68°r6 yield). 'H NMR was consistent with the proposed structure.
is to 3 Preparation of 9ocN~
h Ill C
H
CI y.
To a solution of the product from Step 2 (23.3 g, 72.1 mmol) in DMF
20 (200 mL) was added N-t-Boc-glycine N-hydroxysuccinimide ester (17.9 g, 65.9 mmol). The reaction mixture was stirred at room temperature for 20 hours. The mixture was poured into ethyl acetate (1.2 L) and washed with 1M HCI (2 X 250 mL), saturated aqueous NaHC03solution (2 X 250 mL) and brine (2 X 250 mL). The solution was dried (MgS04) and concentrated 2s to give the desired product (32.0 g, 100 % yield).
Anal. calcd for C,eH=,BrCINZOs: C, 45.06; H, 5.04; N, 5.84.
Found: C, 45.17; H, 5.14; N, 6.12.
'H NMR was consistent with the desired structure.

to 4 HZN

~ HC1 CI
To a solution of the product of Step 3 (31.9 g, 6fi.5 mmol), in s absolute ethanol (205 mL) was added an ethanolic HCI sotutian (111 mL of a 3M solution, 332.4 mmol). The reaction solution was heated at 58°C
for 30 minutes. The solution was cooled and concentrated in vacuo. The residue was dissolved in ethyl acetate (250 mL) and stirred at 0°C for hours. A white precipitate was collected by filtration and washed with cold to ethyl acetate. The solid was dried in vacuo to give. the desired product (23.5 g, 85°~ yield).
Anal. calcd for C,aH,sBrCIN?0, + 1.0 HCI: C, 37.53; H, 4.12; N, fi.73.
Found: C, 37.29; H, 4.06; N, 6.68.
'H NMR was consistent with the desired structure.

EXAMPLE L
Preparation of C02Et ~N~ NH
O / ( OH ~ HCl Br ~ CI
~teD 1 Preparation of H O
Br ~ CI
Potassium carbonate (powder, oven dried at 100°C under vacuum, io 22.1 g, 0.16 moles) was added to a solution of 3-chloro-5-brornosalicyialdehyde (35.0 g, 0.15 moles) in DMF (175 ml) at room temperature to give a bright yellow slurry. MEMCI (neat, 25.0 g, 0.2 moles) was then added while maintaining the bath temperature at 20°C. The mixture was then stirred at 22°C for 6 hours and was poured into DI
water ~s (1200mL) to precipitate the product. The slurry was filtered on a pressure filter and the cake was washed with DI water (2 x 400 mL) and was dried under N2lvacuum to afford the product (46.Og, 95 °~ yield) es an off white solid. 'H NMR (CDCI,, TMS) 3.35 (s, 3H), 3.54 to 3.56 (m, 2H), 3.91 to 3.93 (m, 2H), 5.30 (s, 2H), 7.77 (d, 1 H), 7.85 (d, 1 H), 10.30 (s, 1 H); "C
2o NMR (CDC13, TMS) (ppm):59.05, 70.11, 71.49, 99.50, 117.93, 129.69, 129.78, 132.37, 138.14, 155.12, 188.22. DSC: 48.24°C (endo 90.51JIg);
Microanalyticai: calcd for C"H,zBrClO,:
C: 40.82%; H: 3.74%; CI: 10:95%; Br: 24.fi9%;
found: C: 40.64%; H:3.48°~; CI: 10.99°~; Br 24.67°~.
2s VI~O 99/44994 PGT/US99/03281 Preparation of Hr s The product from Step 1 (32.35 g., 0.1 mol) was charged in a 500 ml 3N round bottom flask fitted with a mechanical stirrer, followed by addition of THF (160 ml) and (S)-phenylglycinol (13.71 g, 0.1 mol). After 30 minutes at 22°C, MgSO, (20 g.) was added. The mixture was stirred for 1 hour at 22°C and filtered on a coarse fritted filter. The fittrate was ><o concentrated under reduce pressure to afford a pale yellow oil (48.0 g) containing the desired imine. No further purification was performed and the crude product was used directly in the next reaction step.
Microanalytical: calcd for C,sH2,BrCINO,:
C: 51.54%; H: 4.78%; N: 3.16%; Br: 18.04°h; CI: 8.00%
1s found: C: 51.52°~; H: 5.02%; N: 2.82%; Br: 16.31 °~; C1:7.61 °~.
to 3 Preparation of OH ~ \
~O NH
O ~ ~ O\/O\/\O/
Br Cl In a 5L 3N round bottom flask fitted with a mechanical stirrer, reagent from Example I, Step 1, (332 g, 0.8 mol) was taken up in NMP

(660 mL) under nitrogen. The solution was then cooled to -10°C. A
solution of imine produced in Step 2, in NMP (320 ml) was prepared under nitrogen and then added over 30 minutes to the above reaction mixture while the temperature was maintained at -5°C. The mixture was stirred for s an additional hour after the addition was complete and cooled to -10°C. A
mixture of concentrated HCl/saturated solution of NH~CI (30mU720 mL) was added over 10 minutes. MTBE (760 ml) was added and the mixture was stirred for 1 hour at 23°C. Stirring was stopped and the layers were separated. The aqueous layer was extracted with MTBE (320 ml). The ~o two organic layers were combined, washed successively with a saturated solution of NH,CI (320 ml), DI water (320 ml) and brine (320 ml). The solution was dried with MgSO~ (60 g), filtered and concentrated to afford a yellow oil (228 g) containing the desired product as a single diastereoisomer. DSC: 227.54°C (endo. 61.63 J/g);
is Microanalytical: calcd for CnH"BrCIN06:
C: 53.72%; H: 5.95%; N: 2.50°h; Br: 14.29%; CI: 6.33%
found: C: 53.80%; H: 6.45%; N: 2.23%; Br: 12.85%; Ci:fi.12%.
to 4 Preparation of i I
a~
A solution of crude ester produced in Step 3 (~-111 g) in ethanol 2s (1500 mL) was charged under nitrogen atmosphere to a 3L 3N round bottom flask fitted with a mechanical stirrer. The reaction mixture was cooled to 0°C and lead tetraacetate (88.67 g, 0.2 mol) was added in one portion. The reaction mixture was stirred for 3 hours at 0°C and then 15°h aqueous NaOH (150 mL) was added to the reaction mixture below 5°C.
The ethanol was removed under reduced pressure on rotavap. Another 600 mL of 15% aqueous NaOH was added and the reaction mixture was extracted with ethyl acetate (2 x 300 mL), MTBE (2 x 200 mL ) and ethyl s acetate {2 x 200 mL). The organic layers were combined and washed with DI water (2 x 200 mL) and brine (2 x 100 mL) and dried over anhydrous MgSO~ {30 g). The solution was then filtered over celite and concentrated under reduced pressure to give the product as an orange oil (96 g) that was used in the next step without further purification.
1o OSC: 233.60°C tondo. 67.85 Jlg);
Microanalytical: calod far C2,H~9BrCIN05:
C: 54.71 %; H: 5.54%; N: 2.65°~; Br: 15.16%; CI: 6.72%
found: C: 52.12°~; H: 5.40°h; N: 2.4?%; Br: 14.77°~;
C1:6.48%.
1s to 5 Preparation of ~N C02Et SDaH
OH , 'i Br CI CH3 2o The crude product from Step 4 {-94 g) was taken up in absolute ethanol (180 mL) and para toluenesuffonic acid monohydrate (50.0 g, 0.26 mot) was added. The reaction mixture was then heated to reflux for 8 hours after which the solvent was removed under reduced pressure. The residual solid was taken up in THF (100 mL) and the THF was then 2s stripped off under reduced pressure. The residue was dissolved in ethyl acetate (500 mL) and cooled to -5°C. The solid was filtered and washed with heptane (2 x 50 mL) to give a white solid. The solid was then air dried to give the desired product as a white solid (38 g) as a single isomer. 'H

NMR (DMSO, TMS) (ppm) 1.12 (t, 3H), 2.29 (s, 3H), 3.0 (m, 2H), 4.05 (q, 2H),4.88 (t, 1 H), 7.11 (d, 2H), 7.48 (d, 2H), 7.55 (d, 1 H), 7.68 (1 H, d), 8.35 (br. s, 3H) ; "C NMR (DMSO, TMS) (ppm):13.82, 20.75, 37.13, 45.59, 60.59, 110.63, 122.47, 125.44, 127.87, 128.06, 129. 51, 131.95, 137.77, S 145.33, 150.14, 168.98; DSC:69.86°C (end., 406.5 JI9), 165.72°C (end.
62.27 J/g), 211.24°C (exo. 20.56 Jlg) (aJ~25= + 4.2° (c =0.960;Me0H); lR
(MlR) (cm-1 ) 2922, 1726, 1621, 1591, 1494, 1471, 1413, 1376, 1324, 1286, 1237, 1207;. MicroanalytiCal: calcd for C,aH2,BrCINOsS:
C: 43.69%; H:4.27%; N: 2.83%; Br: 16.15%, CI: 7.16°I6, S: 6.48%
to found: C: 43.40%; H:4.24%; N: 2.73%; 8r: 16.40%, CI: 7.20%, S: 6.54%.
to 6 Preparation of COZEt HZN~ NH
O ~ I OH ° HC!
15 Br ~ CI
The above compound was prepared according to the procedures outlined in Example I, Step 4 and Step 5 where an equivalent quantity of the intermediate prepared in Step 5 as the free base is substituted fin 2o Example I, Step 4.
MS and'H NMR were consistent with the desired structure.

WO 99!44994 PCTNS99/03281 EXAMPLE M
Preparation of H
H2N~ N COZEt O / OH
~ HC1 CI ~ I
Step 1 s Preparation of 3-lodo-5-chlorosalicylaldehyde N-lodosuccinimide (144.0 g, 0.641 mole) was added to a solution of 5-chlorosalicytaldehyde (100 g, 0.638 mole) in dimethylformamide (400 mL). The reaction mixture was stirred for 2 days at room temperature.
io Additional N-iodosuccinimide (20.0 g) was added and stirring was continued for additional 2 days. The reaction mixture was diluted with ethyl acetate (1 L), washed with hydrochloric acid (300 mL, 0.1 N),~water (300 mL), sodium thiosulfate (5%, 300 mL), brine (300 mL), dried (MgSO,) and was concentrated to dryness to afford the desired aldehyde as a pale 15 yellow solid (162 g, 90°~ yield).
MS and'H NMR were consistent with the desired structure.
to 20 Preparation of 2-O-(MEM)-3-iodo-5-chlorosalicylaldehyde Potassium carbonate (41.4 g, 0.30 mole) was added to a solution of 3-iodo-5-chlorosalicylaldehyde (84.74 g, 0.30 mole) in DMF (200 mL) at 20°C. This resulted in a yellow slurry and MEM-CI (38.2 g, 0.305 mole) was added maintaining the reaction temperature. After 2 hoursa additional 2s MEM-CI (1.5 g) was added. After stirring for 1 hour, the reaction mixture was poured into an ice-water mixture and was stirred. The precipitate formed, was filtered, and was dried in vacuo to afford the desired protected aldehyde. Yield: 95 g (85°,~).
MS and'H NMR were consistent with the desired structure.

Step 3 Preparation of COzt-Bu HO ~ I O~O~pi Ct ~ 1 (S)-Phenyl glycinol (15.37 g, 0.112 mole) was added to a solution of 2-O-(MEM)-3-iodo-5-chlorosalicylaldehyde (41.5 g, 0.112 mole) in THF
(200 mL) at room temperature. After 1 hour of stirring MgSO, (16 g) was added and the stinging was continued for 2 hours. The reaction mixture io was filtered and the filtrate was concentrated and was dried in vacuo for 2 hours to obtain the desired intermediate imine. A 2-neck round bottomed flask was charged with the Reformatsky reagent from Example I, Step 1, (81.8 g, 0.2464 mote) and N-methylpyrrolidone (300 mL) and was stirred at -10°C. A solution of the imine in N-methylpyrrolidone (100 mL) was slowly is added maintaining the temperature at -10°C. The mixture was maintained at this temperature for 2 hours and for 1 hour at -5°C.. After cooling the reaction mixture to -10°C, a solution of concentrated HCI in saturated ammonium chloride (16 mU200 mL) was added. Ethyl ether (500 mL) was added and was stirred for 2 hours at room temperature. The ether layer 2o was separated, and the aqueous layer was further extracted with ether (300 mL). The combined ether layers were washed with saturated ammonium chloride (200 mL), water (200 mL), brine (200 mL), dried (MgSO,) and concentrated to afford an oil (61.0 g, 90°~ yield). '1-1 NMR
indicated that the desired structure was substantially one diastereomer 2s and MS was consistent with the desired structure.

Step 4 Preparation of ..COZEt CI

s A sotution of the cnrde ester produced in Step 3 (48.85 8, 80.61 mmol) was dissolved in ethanol (500 mL) and was cooled to 0°C. Lead tetraacetate (35.71 g, 80.61 mmol) was added. After 3 hours, 15 solution of NaOH (73 mL) was added to the reaction mixture. Most of the ethanol was removed under reduced pressure. To the residue was added 1o a 15°~ solution of NaOH (200 mL) and which was then extracted with ether (400 mL). The ether layer was washed with water (100 mL), brine (100 mL), dried and was concentrated to afford an orange oil. The oil was dissolved in ethanol (100 mL) and para-toiuenesulfonic acid (19.9 g) was added. The solution was heated at reflex far 8 hours and was 1s concentrated under reduced pressure. The residue was diluted with THF
(60 mL) and was heated at reflex and was cooled. The precipitate was filtered, washed with hexanelTHF (300 mL, 1:1 )and dried to afford the desired product.
MS and'H NMR were consistent with the desired structure.
2o to 5 S - Ethyl 3-(N-BOC-giy)-amino-3-(S)-(5-chloro-2-hydroxy-3-iodo)phenyl propionate . .
To a mixture of BOC-gly-OSu (9.4 g, 34.51 mmol), ethyl 3-(S)-2s amino-3-(5-chloro-2-hydroxy-3-iodo) propionate PTSA salt (17.0 g; 31.38 mmol) in DMF (200 mL) was added triethylamine (4.8 mL). The reaction mixture was stirred for 18 hours at room temperature. The DMF was removed in vacuo and the residue was partitioned between ethyl acetate '70 (600 mL) and diluted hydrochloric acid (100 mL). The organic layer was washed with sodium bicarbonate (200 mL), brine (200 mL), dried (MgSO~) and was concentrated to afford of the desired product as a solid (14.2 g, 86°~ yield).
s MS and'H NMR were consistent with the desired structure.
to 6 S - Ethyl 3-(N-gly)-amino-3-(5-chloro-2-hydroxy-3-iodo)phenyl propionate to hydrochloride DioxaneIHCI (4N, 70 mL) was added to ethyl 3-(S)-(N-BOC-gly)-amino-3-(5-chioro-2-hydroxy-3-iodo)phenyl propionate (37.20 g, 70.62 mmol) at 0°C and was stirred at room temperature for 3 hours. The is reaction mixture was concentrated, and concentrated once more after addition of toluene (100 mL). The residue obtained was suspended in ether, was filtered and dried to afford the desired product as a crystalline powder (32.0 g, 98°~ yield).
MS and'H NMR were consistent with the desired structure.

EXAMPLE N
Preparation of NH
N2N~ COZEt OH
~ I ~ HCI
8r ~ Br to 1 s Preparation of H O
O~O~O/
Br ~ Br The above compound was prepared according to Example I, Step 2A, substituting an equivalent quantity of 2-hydroxy-3,5-to dibromobenzaidehyde for 3,5-dichlorosalicylaldehyde.
Yield 88°r6; Pale yellow solid; m. p. 46-47 °C; Rr = 0.6 (EtOAdHexane 1:1 vlv);'H-NMR (CDCh) d 3.37 (s, 3H), 3.56 (m, 2H), 3.92 (m, 2H), 5.29 (s, 2H), 7.91 (d, 1 H, J = 2.4 Hz), 7.94 (d, 1 H, J = 2.4 Hz), 10.27 (s, 1 H); FAR-MS m/z 367 (M') is HR-MS calculated for C"H,ZBrzO, 367.9083 found 367.9077.
MS and'H NMR were consistent with the desired structure.
Step 2 \' OH
O ,,,. NH

2o Br '~ Br The above compound was prepared using the procedure of Example I, Step 2B and Step 2C, substituting an equivalent quantity of the compound of Step 1 in Example I, Step 2B.
Yield 90°~; Yellow solid; m. p. 57-59 °C; Rr = 0.46 (EtOAdHexane 1:1 s vlv); 'H-NMR (CDCI~) d 1.45 (s, 9H); 2.1 (br, 1H, exchangeable), 2.51 (d, 1 H, J, = 9.9 Hz, Js = 15.3 Hz}, 2.66 (d, 1 H, J, = 4.2 Hz, Jz = 15.3 Hz}, 3.02 (br, 1 H, exchangeable), 3.39 (s, 3H), 3.58 - 3.62 (m, 4H), 3.81 (m, 1 H), 3.93 (m, 2H), 4.63 (dd, 1 H, J = 4.2 Hz), 5.15 (s, 2H), 7.17-7.25 (m, 6H), 7.49 (d, 1 H); FAB- MS mfz 602 (M+H) to HR-MS calculated for C~sH~,NBrzOs fi02.0753 found 602.0749.
MS and'H NMR were consistent with the desired structure.
to is Preparation of O ,,,,,, NH2 03H
O OH
8 ~ Br CH3 The above compound (p-toluenesutfonate salt) was prepared 2o according to Example 1, Step 3 by substituting an equivalent quantity of the product prepared in Step 2 in Example I, Step 3A. Yield 62%; white solid;
'H-NMR (DMSO-ds) d 1.09 (t, 3H, J = 7.2 Hz), 2.27 (s, 3H), 2.97(dd, 2H, J, = 3.0 Hz, J2 = 7.2 Hz), 4.02 (q, 2H, J = 7.2 Hz), 4.87 (t, 1 H, J = 7.2 Hz), 7.08 (d, 2H, J = 4.8 Hz), 7.45 (m, 3H), 7.57 (d, 1 H, J = 2.4 Hz), 8.2 (br,3H);
2s FAB-MS mh 365 (M+H) HR-MS calculated for C"H,,,NBr203, 365.9340 found 365.9311.
MS and'H NMR were consistent with the desired structure.

to 4 Preparation of COzEt 'N
HZ ~(N
O nu ~ HC1 Br s The above compound was prepared using the procedure of Example I, Step 4 substituting the compound prepared in Step 3. The resulting BOC protected intermediate, was converted to the desired compound using the procedure of Example I, Step 5.
MS and'H NMR were consistent with the desired structure.

VKO 99/44994 PtrTIUS99/03281 EXAMPLE P
Preparation of H C02Et H2N ~ N
OH
~ HCl 8r 1' I
The above compound is prepared according to the procedure of Example I by substituting an equivalent amount of 3-iodo-5-bromosalicytatdehyde prepared in Example F, Step 1 for 3,5-dichlorosalicytatdehyde in Example I, Step 2 A.

(t) 3-bromo-5-chloro-2-hydroxy-[3-[[2-[j[3-hydroxy-5-[(1,4,5,6-tetrahydro-5-hydroxypyrimidin-2-yl)amino)phenyl)carbonyljamino]-acetyl]aminojbenzenepropanoic acid, trifluroacetate salt Preparation of O
N N CO=H
1C' ~ H'1~
Ho ~ o ~,. off OH
CI ~ 8r io To the product of Example H (0.4 g, 0.0014 mole), the product of Example B (0.58 g, 0.0014 mole), triethylamine (0.142 g, 0.0014 mote), DMAP (17 mg), and anhydrous DMA (4 ml) was added EDCI (0.268 g, 0.0014 mole) at ice bath temperature. The reaction was stirred ovemi8ht at room temperature. The resulting ester intermediate was isolated by ~s reverse phase preparatory HPLC. To this ester in H20 (10 ml) and CH$CN
(5 ml) was added LiOH (580 mg, 0.0138 mole). After stirring at room temperature for 1 hour, the pH was lowered to 2 with TFA and the product was purified by reverse phase preparatory HPLC to yield (after lyophilization) the desired product as a white solid (230 mg).
2o MS and'H NMR were consistent with the desired structure.

Preparation of N
~ ~ ~ cozH
I
N O /", OH
HO I
OH
CI CI
The above compound was prepared according to the methodology of Example 1, substituting an equivalent amount of the product from Example A for the product from Example B.
The yield, after lyophilization was 320 mg of as a white solid.
io MS and'H NMR were consistent with the desired structure.

Preparation of N. N N~ N C02H

N O ~ OH
HO
8r I
The above compound was prepared according to the methodology of Example 1, substituting an equivalent amount of the product from Example F for the product from Example B. The yield (after lyophilization) was 7 80 mg as a white solid.
1o MS and'H NMR were consistent with the desired stnrcture.

Preparation of N N ~ N N CO?H
1' ~ H''~
HO N ~"~ O / OH
OH
Br CI
The above compound was prepared according to the methodology of Example 1, substituting an equivalent amount of the product of Example D for the product of Example B. The yield (after iyophiliZation) was 180 mg as a white solid.
io MS and'H NMR were consistent with the desired structure.

Preparation of i H'1r HO N O ~,. OH
OH
ci i The above compound was prepared according to the methodology of Example 1, substituting an equivalent amount of the product from Example E for the product from Example B. The yield (after lyophilization) was 250 mg as a white solid.
io MS and'H NMR were consistent with the desired structure.
so VItU 99/44994 PCTNS99/03281 Preparation of N N COzH

8r ~ Bf The above compound was prepared according to the methodology of Example 1, substituting an equivalent amount of the product from Example C for the product from Example B. The yield (after iyophifization) was 220 mg as a white solid.
io MS and'H NMR were consistent with the desired product.

~~MPI~.E 7 Preparation of N N NH
H~ CpZH
HO I' / OH
OH
sr ''~ cl s To the product from Example H (7.8 g, 0.027 mole) dissolved in anhydrous DMA (50 mL) in a flame dried flask under N2 and at ice bath temperature was slowly added isobutytchloroformate (3.7 g, 0.027 mole) followed by N-methylmorpholine (2.73 g, 0.027 mole). The solution was ~o stirred at ice bath temperature for 15 minutes. To the reaction mixture was then added the product from Example L (10.0 g, 0.024 mole) at ice bath temperature followed by N-methylmorpholine (2.43 g, 0.024 mole).
The reaction was then stirred at room temperature overnight. The resultinD ester intermediate was isolated by reverse phase prep HPLC. To is the ester in Hz0 (60 mL) and CH,CN (30 mL ) was added LiOH (10 g, 0.238 mole). The reaction mixture was stirred at room temperature for 1 hour. The pH was then lowered to 2 with TFA. The product was purified by reverse phase prep HPLC to yield (after lyophilization) the desired product as a white solid (9.7 g).
2o MS and'H NMR were consistent with the desired structure.

(S) 3,5-dichloro-2-hydroxy-~-[[2-[[[3-hydroxy-5-[(1,4,5,6-tetrahydro-5-hydroxypyrimidin-2-yl)amino]phenyl]carbonyl]amino]acetyl]amino]-benzenepropanoic acid, monohydrochloride monohydrate s Preparation of H H
N H N C~H
HO N O / OH . HC I
CI ~ CI
to A
io To the product from Example H (9.92 g, 0.0345 mole) dissolved in anhydrous DME (200 mL) is added N-methytmorpholine (4.0 mL, 0.03fi2 mole). The reaction mixture was cooled to -5°C (salt-ice bath).
isobutylchioroformate, IBCF (4.48 mL, 4.713 g, 0.0345 mole) was added over one minute and the reaction mixture stirred at ice bath temperature is for 12 minutes. To the reaction mixture was then added the product from Example I ( 11.15 g, 0.030 mole) at ice bath temperature followed by N-methylmorpholine (4.0 mL, 0.0362 mole). The reaction mixture was allowed to warm to room temperature and go to completion then concentrated under vacuum at 50°C to give a dark residue. The residue 2o was dissolved in acetonitrile : H20 (about 50 mL). The pH was made acidic by addition of a small amount of TFA. The residue was placed on a x 500 cm C-18 (50 a particle size) column and the ester of the desired product isolated. (Solvent program: 100% Hz0 + 0.05% TFA t~o_30:70 HZO
+ 0.05°~ TFA : acetonitrile + 0.05% TFA over 1 hour @ 100 mUminute: the 2s solvent program was initiated after the solvent front elutes). Preparatory RPHPLC pur~cation resulted in a white solid (10.5 g) after lyophilization (50°i6).
MS and'H NMR were consistent with the desired structure.

VI!O 99/44994 PCTIUS99/03281 The product produced in Step A (about 11 g) was dissolved in dioxane : water and the pH of the solution adjusted to approximately 11.5 (pH meter) by the addition of 2.5 N NaOH. The reaction mixture was s stirred at room temperature. Periodically, the pH was re-adjusted to > 11 by further addition of base. After 2-3 hours the conversion of ester to acid was deemed complete by RPHPLC. The pH of the reaction mixture was adjusted to about 6 and a viscous oil precipitated from solution. The oil was isolated by decantation and washed with hot water (200 mL). The io resulting aqueous mixture was allowed to coot and the solid was collected by filtration to yield The above compound {2.6 g after lyophitization from HCI solution). The residue, which was a dark viscous oil was treated with hot water to give on cooling a tan powder (4.12 g after lyophilization from HCI solution).
MS and'H NMR were consistent with the desired structure.

(S) 3-bromo-5-chloro-2-hydroxy-~-[[2-[[[3-hydroxy-5-[(1,4,5,6-tetrahydro-5 hydroxypyrimidin-2-yl)amino]phenyljcarbonyljamino]acetyljamino]
benzenepropanoic acid, trifluoroacetate salt s Step 1 - Preparation of H H
~'N C02Et N 0 ~ OH
HO
OH I /
C1 Br To a suspension of the product from Example J (1.0 g, 2.4 mmol), to the product from Example H (0.75 g, 2.6 mmol) and 4-dimethylaminopyridine (40 mg) in N,N-dimethylacetamide (10 mL) was added triethylamine (0.24 g, 2.4 mmol). The mixture was stirred at room temperature for 15 minutes and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (0.60 g, 3.1 mmol). The reaction mixture is was stirred at room temperature overnight. The mixture was concentrated in vacuo and purified by reverse phase HPLC (starting gradient 90:.10 H20lTFA:MeCN, retention time 22 minutes) to give the desired product, (1.6 g, 52% yield).
'H NMR was consistent with the proposed structure.
to 2 H . 0 H
N~ H~N C02H
N O ~ OH
HO
TFA
CI"' ''~'~ ~Br To a solution of the ester produced in Step 1 (800 mg, 1.2 mmol) in a 1:4 MeCN:HzO solution (7 mL) was added lithium hydroxide (148 mg, 6.2 mmol). The reaction mixture was stirred at room temperature for 2 hours.
TFA (0.71 mL, 9.2 mmol) was added and the mixture purified by reverse s phase HPIC (starting gradient 95:5 H~OITFA:MeCN, retention time 24 minutes) to give the desired product (860 mg,~83°~ yield).
Anal. calcd for C~Hz~BrCINsO~ + 1.7 TFA:
C, 39.18; H, 3.20; N, 8.99.
Found: - C, 39.11; H, 3.17; N, 9.07.
to MS and'H NMR were consistent with the desired structure.
to 3 Preparation of the hydrochloride salt is The product of Step 2 was dissolved in a suitable solvent (acetontrile : water) and the solution slowly passed through a Bio-Rad AG2 - 8X (chloride form, 200 - 400 mesh, > 5 equivalents) ion-exchange column. Lyophilization gives the desired product as an HCI salt.

!EXAMPLE 10 Preparation of H H
N N N COZH .

HO Y
OH
B
s The above compound was prepared using the procedure of Example 8 substituting the product of Example N for the product of Example I in Example 8, Step A. The product was isolated by prep RPHPLC and lyophilized to give the desired product as a TFA salt.
to MS and'H NMR were consistent with the desired structure.
s~

F~~ ~ 1 Preparation of H H
N H~N COxH
HO N O , OH
CI ~ 1 The above compound was prepared using essentially the procedures of Example 8 and substituting the product of Example M for the product of Example I in Example 8, Step A. The product was isolated io by preparatory RPHPLC and lyophilized to give the desired product as a TFA salt.
MS and'H NMR were consistent with the desired structure.

Preparation of H
N" H~NH C~H

HO
OH Br ~ t The above compound was prepared using the procedures of Example 8 and substituting the product of Example P for the product of Example I in Example 8, Step A. The product is isolated by prep RPHPLC
io and lyophilized to give the desired product as a TFA raft.
89.

~,~CAMPLE 13 Preparation of N N N
COOH
N O ~ OH
HO
OH I /
''. ~ I
s Preparation of 2-O-(MEM)-3,5-diiodosalicylaldehyde CHO
w 0~./0~0/
I
Potassium carbonate (18.5 g, 0.134 mole) was added to a solution of 3,5-diiodosalicylaldehyde (50.0 g, 0.134 mole) in DMF (150 mL) at 20°C. This resulted in a yellow slurry and MEM-CI (15.8 mL, 0.134 mole) 1o was added maintaining the reaction temperature. After 2 hours, additional MEM-CI .(1.5 g) was added. After stirring for a further 1 hour, the reaction mixture was poured into ice-water and stirred. The precipitate formed, was filtered, and dried in vacuo to afford the desired protected aldehyde. (61 g, 99°~ yield). 'H NMR was consistent with the desired product.
is to Preparation of O~O/
n n (S)-phenyl glycinol (17.9 g, 0.13 mole) was added to a solution of 2-O-(MEM)-3,5-diiodosalicylaldehyde (41.5 g, 0.112 mole) in THF (150 mL) at room temperature. After 1 hour of stirring MgSO, (20.7 g) was added and the stirring was continued for 2 hours. The reaction mixture was s filtered and the filtrate was concentrated and dried in vacuo for 2 hours. A
2-neck round bottomed flask was charged with the Refonnatsky reagent (96 g, 0,289 mole) and N-methylpyrrolidone {250 mL) and wes stirred at -10°C. A solution of the imine in N-methylpyrrolidone (100 mL) was slowly added maintaining the temperature at -10°C. The mixture was maintained 1o at this temperature for 2 hours and for 1 hour at -5°C. After cooling the reaction mixture to -10°C, a solution of concentrated HCI in saturated ammonium chloride (1fi mI1200 mt) was added. Ethyl ether (500 mL) was added and the mixture was stirred for 2 hours at room temperature. The ether layer was separated, and the aqueous layer further extracted with 1s ether (300 mL). The combined ether Payers were washed with saturated ammonium chloride {200 mL), water (200 mL), brine (200 mL), dried (MgSO,) and concentrated to afford an oil (90.0 g, 99% yield). NMR
indicated desired product and one diastereomer.
2o Steo 33 Preparation of HZN
'COOH
OH
/ ( ' OH
I I / S/
0~ ~~
O
A solution of the crude ester from Step 2 (14.0 g, 20.1 mmol) was 2s dissolved in ethanol (100 mL) and was cooled to 0°C. Lead tetra acetate (9.20 g, 20.75 mmol) was added in one lot, After 3 hours, 15% solution of NaOH (73 mL) was added to the reaction mixture. Most of the ethanol was VIiO 99/44994 PCTIUS99/03281 removed under reduced pressure. The residue was added to a 15~
solution of NaOH (200 mL) which was extracted with ether (400 mL). The ether Layer was washed with water (100 mL), brine (100 mL), dried and concentrated to afford an orange oil. This was dissolved in ethanol (100 s mL) and para toluenesulfonic acid (6.08 g) was added. The solution was heated at reflux for 8 hours and was concentrated under reduced pressure.
The residue was diluted with THF (60 mL), was heated at reflux and was cooled. Upon storage, no precipitate formed. The reaction mixture was concentrated and purified by preparative hplc to afford the amino acid as to its PTSA salt. The solid obtained was dissolved in ethanol and was saturated with HCI gas. The reaction mixture was heated at reflux for 6 hours. The reaction mixture was concentrated to afford the PTSA salt of the desired amino acid (12.47 g).
is t 4 Preparation of Ethyl 3-(N-BOC-gly)-amino-3-(S)-(3,5-diiodo-2-hydroxyphenyl)propionate O H
N
0 NH~'' COOH
0 ~ OH
i ~ I
2o To a mixture of BOC-gly-OSu (7.48 g, 27.04 mmol), ethyl 3-(S)-amino-3-(3,5-diiodo-2-hydroxyphenyl)propionate PTSA salt (12.478, 27.04 rnmol) in DMF (100 mL) was added triethylamine (3.8 mL). The reaction mixture was stirred for 18 hours at room temperature. The DMF.was removed in vacuo and the residue partitioned between ethyl acetate (600 25 mL) and dilute hydrochloric acid ( 100 mL). The organic layer was washed with sodium bicarbonate (200 mL), brine (200 mL), dried (MgS04) and concentrated to afford the desired product as a solid (17.0 g, 96°~
yield).
'H NMR was consistent with the desired product.

Preparation of ethyl 3-(N-gly)-amino-3-(3,5-diiodo-2-hydroxypheny!)-propionate hydrochloride H
HZN~N COOH
0 ,,1 OH
HCI I
I
To dioxaneIHCI (4N, 40 mL) was added ethyl 3-(N-80C-gly)-amino-3-(S)-(3,5-diiodo-2-hydroxyphenyl)propionate (17.0 g, 25.97 mmol) at 0°C
and the reaction mixture was stirred at room temperature for 3 hours. The reaction mixture was concentrated, and concentrated once more after to addition of toluene (100 mL). The residue obtained was dried to afford the desired product as a crystalline powder (8.0 g, 56% yield).'H NMR is consistent with the desired product.
to 6 is A solution of m-(5-hydroxypyrimidino)hippuric acid (3.74 g, 12.98 mmol) in dimethylacetamide (25 mL) was heated until all the material had dissolved. This was then cooled to 0°C and isobutylchloroformate (1.68 mL) was added in one portion followed by N-methylmorpholine (1.45 mL).
After 10 minutes, ethyl 3-(N-gly)-amino-3-(3,5-diiodo-2-hydroxyphenyl)-2o propionate hydrochloride (6.0 g, 10.82 mmol) was added in one portion followed by N-methylmorphoiine (1.45 mL). The reaction mixture was stirred for 18 hours at room temperature. The reaction mixture was concentrated, the residue dissolved in tetrahydrofuran/water (1:1, 20mL), and was chromatographed (reverse phase, 95:5 water: acetonitrile over fi0 2s minutes to 30:70 water: acetonitrile containing 0.1 % TFA). The combined fractions were concentrated. The residue was dissolved in acetonitrile water and lithium hydroxide was added until basic. The solution was stirred for 2 hours. The reaction mixture was concentrated and was w0 99/44994 PCTNS99103281 purified as above by hplc to afford the desired acid as the TFA salt. The TFA salt was converted to the corresponding hydrochloride salt by passing through an ion-exchange column followed by iyophilization. 'H NMR was consistent with the desired product.
s The compounds of the formula VII, VIII, IX, 3~I and 3~IV and isomers thereof, can be prepared according to the methodology disclosed herein substituting the appropriate starting materials and reagents as would be apparent to one with ordinary skill in the art.

The activity of the compounds of the present invention was tested in the following assays. The results of testing in the assays are tabulated in Table 1.
s VITRONECTIN ADHESION ASSAY
MATERIALS
Human vitronectin receptor (a"[i3) was purified from human placenta as previously described [Pytela et al., Methods in ~nzvmoloav, 144:475-489 (1987)]. Human vitronectin was purified from fresh frozen plasma as to previously described [Yatohgo et al., Cell Structure and Function, 13:281-292 (1988)]. Biotinylated human vitronectin was prepared by coupling NHS-biotin from Pierce Chemical Company (Rockford, IL) to purified vitronectin as previously described [Charo et al., J. Biol. Chem., 26fi(3):1415-1421 (1991 )]. Assay buffer, OPD substrate tablets, and RIA
is grade BSA were obtained from Sigma (St. Louis, MO). Anti-biotin antibody was obtained from Calbiochem (La Jolla, CA). Linbro microtiter plates were obtained from Flow Labs (McLean, VA). ADP reagent was obtained from Sigma (St. Louis, MO).
METHODS
Solid Phase Receptor Assays 2s This assay was essentially the same as previously reported [Niiya et al., lood, 70:475-483 (1987)]. The purified human vitronectin receptor (a"[i3) was diluted from stock solutions to 1.0 glmL in tris-buffered saline containing 1.0 mM Ca++, Mg++, and Mn++, pH 7.4 (TBS+++). The diluted receptor was immediately transferred to Linbro microtiter plates at 100 3o Uwell (100 ng receptorlwell). The plates were sealed and incubated overnight at 4°C to allow the receptor to bind to the wells. All remaining steps were at room temperature. The assay plates were emptied and 200 L

of 1°~ RIA grade'BSA in TBS+++ (TBS+++IBSA) were added to block exposed plastic surfaces. Following a 2 hour incubation, the assay plates were washed with TBS+++ using a 96 well plate washer. Logarithmic serial dilution of the test compound and controls were made starting at a stock s concentration of 2 mM and using 2 nM biotinylated vitronectin in TBS+++IgSA as the diluent. This premixing of labeled ligand with test (or control) ligand, and subsequent transfer of 50 L aliquots to the assay plate was carried out with a CETUS Propette robot; the final concentration of the labeled ligand was 1 nM and the highest concentration of test compound io was 1.0 x 10'' M. The competition occurred for two hours after which all wells were washed with a plate washer as before. Affinity purified horseradish peroxidase labeled goat anti-biotin antibody was diluted 1:3000 in TBS+++IBSA and 125 L were added to each well. After 30 minutes, the plates were washed and incubated with OPDIH20 substrate in 100 mMIL
is Citrate buffer, pH 5Ø The plate was read with a microtiter plate reader at a wavelength of 450 nm and when the maximum-binding control wells reached an absoround bottomance of about 1.0, the final Ar,SO were recorded for analysis. The data were analyzed using a. macro written for use with the EXCEL spreadsheet program. The mean, standard deviation, and °r6CV
2o were determined for duplicate concentrations. The mean A,~ values were normalized to the mean of four maximum-binding controls (no competitor added)(B-MAX). The normalized values were subjected to a four parameter curve fit algorithm [Rodbard et al., Int. Atomic Energy Aaencv. Vienna, pp 469 (1977)], plotted on a semi-log scale, and the computed 2s concentration corresponding to inhibition of 50°~ of the maximum binding of biotinylated vitronectin (ICso) and corresponding R2 was reported for those compounds exhibiting greater than 50°~ inhibition at the highest concentration tested; otherwise the IC~o is reported as being greater than the highest concentration tested. ~-[[2-[[5-[(aminoiminomethyl)-aminoj-1-30 oxopentyl]aminoj-1-oxoethy(]amino]-3-pyridinepropanoic acid [USSN
08!375,338, Example 1 ] which is a potent a~[33 antagonist (ICso in the range 3-10 nM) was included on each plate as a positive control.

PURIFIED Ilblllla RECEPTOR ASSAY.
MATERIALS
Human fibrinogen receptor (a"b~a) was purled from outdated s platelets. (Pytela, R., Pierschbacher, M.D., Argraves, S., Suzuki, S., and Rouslahti, E. "Arginine-Glycine-Aspartic acid adhesion receptors", ~,Ilethods in Enzvmolow 144(1987):475-489.) Human vitronectin was pu~fied from fresh frozen plasma as described in Yatohgo, T., Izumi, M., Kashiwagi, H., and Hayashi, M., "Novel purification of vitronectin from la human plasma by heparin affinity chromatography," Sell Structure and Function 13(1988):281-292. Biotinylated human vitronectin was prepared by coupling NHS-biotin from Pierce Chemical Company (Rockford, IL) to purified vitronectin as previously described. (Charo, LF., Nannizzi, L., Phillips, D.R., Hsu, M.A., Scaround bottomorough, R.M., "Inhibition of is fibrinogen binding to GP Ilblllla by a GP Ills peptide", J. Biol. Chem.
26fi(3)(1991 ): 1415-1421.) Assay buffer, OPD substrate tablets, and RlA
grade BSA were obtained from Sigma (St. Louis, MO). Anti-biotin antibody was obtained from Calbiochem (La Jolla, CA). Linbro microtiter plates were obtained from Flow Labs (McLean, VA). ADP reagent was obtained 2o from Sigma (St. Louis, MO).
METHODS
2s Solid Phase Receptor Assays This assay is essentialljr the same reported in Niiya, K., Hodson, E., Bader, R., Byers-Ward, V. Koziol, J.A., Plow, E.F. and Ruggeri, Z.M., "Increased surface expression of the membrane glycoprotein Ilblllla complex induced by platelet activation: Relationships to the binding of 3o fibrinogen and platelet aggregation", Blood 70(1987):475-483. The purified human fibrinogen receptor (a,~b~3) was diluted from stock solutions to 1.0 NgImL in Tris-buffered saline containing 1.0 mM Ca++, Mg++, and Mn++, pH 7.4 (TBS+++). The diluted receptor was immediately transferred to Linbro microtiter plates at 100 NLlwell (100 ng receptoNwell). The plates were sealed and incubated overnight at 4°C to allow the receptor to bind to the wells. All remaining steps were at room temperature. The s assay plates were emptied and 200 NL of 1 °~ RIA grade BSA in TBS+++
(TBS+++lBSA) were added to block exposed plastic surtaces. Following a 2 hour incubation, the assay plates were washed with TBS+++ using a 96 well plate washer. Logarithmic serial dilution of the test compound and controls were made starting at a stock concentration of 2 mM and using 2 to nM biotinylated vitronectin in TBS+++lBSA as the difuent. This premixing of labeled ligand with test (or control) ligand, and subsequent transfer of 50 NL aliquots to the assay plate was carried out with a CETUS Propette robot; the final concentration of the labeled ligand was 1 nM and the highest concentration of test compound was 1.0 x 10-4 M. The is competition occurred for two hours after which all wells were washed with a plate washer as before. Affinity purified horseradish peroxidase labeled goat anti-biotin antibody was diluted 1:3000 in TBS+++~BSA and 125 NL
were added to each well. After 30 minutes, the plates were washed and incubated with ODDIHzOz substrate in 100 mMIL citrate buffer, pH 5Ø
2o The plate was read with a microtiter plate reader at a wavelength of 450 nm and when the maximum-binding control wells reached an absoround bottomance of about 1.0, the final A,,so were recorded for analysis. The data were analyzed using a macro written for use with the EXCELT"' spreadsheet program. The mean, standard deviation, and °~CV were 2s determined for duplicate concentrations. The mean A,~ values were normalized to the mean of four maximum-binding controls (no competitor added)(B-MAX). The normalized values were subjected to a four parameter curve fit algorithm, [Robard et al., Int. Atomic Enerqv Agency, Vienna, pp 469 (1977)], plotted on a semi-log scale, and the computed so concentration corresponding to inhibition of 50°~ of the maximum binding of biotinylated vitronectin (ICso) and corresponding R2 was reported for those compounds exhibiting greater than 50°~ inhibition at the highest concentration tested; otherwise the ICS is reported as being greater than the highest concentration tested. ~i-[(2-[[5-[(aminoiminomethyl)amino]-1-oxopentyljaminoj-1-oxoethyl]aminoj-3-pyridinepropanoic acid [USSN
081375,338, Example 1] which is a potent a"~ antagonist (ICso)in the range 3-10 nM) was included on each plate as a positive control.
~luman Platelet Rich Plasma Assavs Healthy aspirin free donors were selected from a pool of volunteers.
1o The harvesting of platelet rich plasma and subsequent ADP induced platelet aggregation assays were performed as described in Zucker, M.B., "Platelet Aggregation Measured by the Photometric Method", ~Ilethods in Enzvmolosw 169(1989):117-133. Standard venipuncture techniques using a buttertly allowed the withdrawal of 45 mL of whole blood into a 60 mL
is syringe containing 5 mL of 3.8°~ trisodium citrate. Following thorough mixing in the syringe, the anti-coagulated whole blood was transferred to a 50 mL conical polyethylene tube. The blood was centrifuged at room temperature for 12 minutes at 200 mg to sediment non-platelet cells.
Platelet rich plasma was removed to a polyethylene tube and stored at 2o room temperature until used. Platelet poor plasma was obtained from a second centrifugation of the remaining blood at 2000 xg for 15 minutes.
Platelet counts are typically 300,000 to 500,000 per microliter. Platelet rich plasma (0.45 mL) was aliquoted into siliconized cuvettes and stirred (1100 rpm) at 37°C for 1 minute prior to adding 50 uL of pre-diluted test 2s compound. After 1 minute of mixing, aggregation was initiated by the addition of 50 uL of 200 uM ADP. Aggregation was recorded for 3 minutes in a Payton dual channel aggregometer (Payton Scientific, Buffalo, NY).
The percent inhibition of maximal response (saline control) for a series of test compound dilutions was used to determine a dose response curve. All 3o compounds were tested in duplicate and the concentration of half-maximal inhibition (ICso) was calculated graphically from the dose response curve for those compounds which exhibited 50% or greater inhibition at the highest concentration tested; otherwise, the ICso is reported as being greater than the highest concentration tested.
oc"~, IIb/llla xam le lC~o nM IC~o nM

1 0.88 310 2 1.04 430 3 23.7 2440 4 2.02 575 2.13 744 6 6.46 919 7 1.01 ~ . 262 8 0.40 131 9 0.37 388 9 HCi 0.82 226.2 2.26 641 12 . 9. 59 1060 ?O1

Claims (36)

What is claimed is:

1. A compound of the formula wherein X and Y are the same or different halo group; R is H or lower alkyl; and pharmaceutically acceptable salts thereof.

2. A compound according to Claim 1 selected from the group consisting of:
wherein R is H or alkyl; or pharmaceutically acceptable salts thereof.

3. A compound according to Claim 1 selected from the group consisting of

4. A pharmaceutical composition comprising a therapeutically effective amount of a compound according to Claim 1 and a pharmaceutically acceptable carrier.

5. A pharmaceutical composition comprising a therapeutically effective amount of a compound according to Claim 2 and a pharmaceutically acceptable carrier.

6. A pharmaceutical composition comprising a therapeutically effective amount of a compound according to Claim 3 and a pharmaceutically acceptable carrier.

7. A method for treating conditions mediated by the .alpha.v.beta.3 integrin in a mammal in need of such treatment comprising administering an effective .alpha.v.beta.3 inhibiting amount of a compound according to Claim 1.

8. A method for treating conditions mediated by the .alpha.v.beta.3 integrin in a mammal in need of such treatment comprising administering an effective .alpha.v.beta.3 inhibiting amount of a compound according to Claim 2.

9. A method for treating conditions mediated by the .alpha.v.beta.3 integrin in a mamma! in need of such treatment comprising administering an effective .alpha.v.beta.3 inhibiting amount of a compound according to Claim 3.

10. A method according to Claim 7 wherein the condition treated is tumor metastasis.

11. A method according to Claim 8 wherein the condition treated is tumor metastasis.

12. A method according to Claim 9 wherein the condition treated is tumor metastasis.

13. A method according to Claim 7 wherein the condition treated is solid tumor growth.

14. A method according to Claim 8 wherein the condition treated is solid tumor growth.

15. A method according to Claim 9 wherein the condition treated is solid tumor growth.

16. A method according to Claim 7 wherein the condition treated is angiogenesis.

17. A method according to Claim 8 wherein the condition treated is angiogenesis.

18. A method according to Claim 9 wherein the condition treated is angiogenesis.

19. A method according to Claim 7 wherein the condition treated is osteoporosis.

20. A method according to Claim 8 wherein the condition treated is osteoporosis.

21. A method according to Claim 9 wherein the condition treated is osteoporosis.

22. A method according to Claim 7 wherein the condition treated is humoral hypercalcemia of malignancy.

23. A method according to Claim 8 wherein the condition treated is humoral hypercalcemia of malignancy.

24. A method according to Claim 9 wherein the condition treated is humoral hypercalcemia of malignanc

25. A method according to Claim 7 wherein the condition treated is smooth muscle cell migration.

26. A method according to Claim 8 wherein the condition treated is smooth muscle cell migration.

27. A method according to Claim 9 wherein the condition treated is smooth muscle cell migration.

28. A method according to Claim 7 wherein restenosis is inhibited.

29. A method according to Claim 8 wherein restenosis is inhibited.

30. A method according to Claim 9 wherein restenosis is inhibited.

31. A method according to Claim 7 wherein the condition treated is rheumatoid arthritis.

32. A method according to Claim 8 wherein the condition treated is rheumatoid arthritis.

33. A method according to Claim 9 wherein the condition treated is rheumatoid arthritis.

34. A method according to Claim 7 wherein the condition treated is macular degeneration.

35. A method according to Claim 8 wherein the condition treated is macular degeneration.

36. A method according to Claim 9 wherein the condition treated is macular degeneration.