CA2293600A1 - Treatment of fabrics, garments, or yarns with haloperoxidase - Google Patents
Treatment of fabrics, garments, or yarns with haloperoxidase Download PDFInfo
- Publication number
- CA2293600A1 CA2293600A1 CA002293600A CA2293600A CA2293600A1 CA 2293600 A1 CA2293600 A1 CA 2293600A1 CA 002293600 A CA002293600 A CA 002293600A CA 2293600 A CA2293600 A CA 2293600A CA 2293600 A1 CA2293600 A1 CA 2293600A1
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- Canada
- Prior art keywords
- fabric
- haloperoxidase
- yarn
- hydrogen peroxide
- garments
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 239000004744 fabric Substances 0.000 title claims abstract description 73
- 238000011282 treatment Methods 0.000 title description 11
- 238000000034 method Methods 0.000 claims abstract description 56
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 50
- 150000004820 halides Chemical class 0.000 claims abstract description 14
- 239000012736 aqueous medium Substances 0.000 claims abstract description 9
- 238000004061 bleaching Methods 0.000 claims description 31
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- 108090000790 Enzymes Proteins 0.000 claims description 23
- 210000002268 wool Anatomy 0.000 claims description 22
- 239000000463 material Substances 0.000 claims description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- 241000371662 Curvularia verruculosa Species 0.000 claims description 10
- 238000004519 manufacturing process Methods 0.000 claims description 8
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- 102000004316 Oxidoreductases Human genes 0.000 claims description 6
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- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims description 6
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 claims description 6
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- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- IOLCXVTUBQKXJR-UHFFFAOYSA-M potassium bromide Chemical compound [K+].[Br-] IOLCXVTUBQKXJR-UHFFFAOYSA-M 0.000 claims description 4
- JHJLBTNAGRQEKS-UHFFFAOYSA-M sodium bromide Chemical compound [Na+].[Br-] JHJLBTNAGRQEKS-UHFFFAOYSA-M 0.000 claims description 4
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- 239000002243 precursor Substances 0.000 claims description 2
- 235000009518 sodium iodide Nutrition 0.000 claims description 2
- MWNQXXOSWHCCOZ-UHFFFAOYSA-L sodium;oxido carbonate Chemical compound [Na+].[O-]OC([O-])=O MWNQXXOSWHCCOZ-UHFFFAOYSA-L 0.000 claims description 2
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- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 2
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- ZBJVLWIYKOAYQH-UHFFFAOYSA-N naphthalen-2-yl 2-hydroxybenzoate Chemical compound OC1=CC=CC=C1C(=O)OC1=CC=C(C=CC=C2)C2=C1 ZBJVLWIYKOAYQH-UHFFFAOYSA-N 0.000 description 2
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- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Chemical class [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 2
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- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
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- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 102000011632 Caseins Human genes 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 1
- 241001537312 Curvularia inaequalis Species 0.000 description 1
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 108010015776 Glucose oxidase Proteins 0.000 description 1
- 239000004366 Glucose oxidase Substances 0.000 description 1
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical class C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
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- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
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- 239000004952 Polyamide Substances 0.000 description 1
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- GOOHAUXETOMSMM-UHFFFAOYSA-N Propylene oxide Chemical compound CC1CO1 GOOHAUXETOMSMM-UHFFFAOYSA-N 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 206010040954 Skin wrinkling Diseases 0.000 description 1
- 239000004115 Sodium Silicate Substances 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 108010073771 Soybean Proteins Proteins 0.000 description 1
- ULUAUXLGCMPNKK-UHFFFAOYSA-N Sulfobutanedioic acid Chemical class OC(=O)CC(C(O)=O)S(O)(=O)=O ULUAUXLGCMPNKK-UHFFFAOYSA-N 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 238000005299 abrasion Methods 0.000 description 1
- WNLRTRBMVRJNCN-UHFFFAOYSA-L adipate(2-) Chemical compound [O-]C(=O)CCCCC([O-])=O WNLRTRBMVRJNCN-UHFFFAOYSA-L 0.000 description 1
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- 150000001336 alkenes Chemical class 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
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- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 1
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- 229910052739 hydrogen Inorganic materials 0.000 description 1
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- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 1
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Landscapes
- Chemical Or Physical Treatment Of Fibers (AREA)
- Detergent Compositions (AREA)
Abstract
A new method of treating undyed fabrics, garments, or yarn comprising treating the undyed fabric, garment, or yarn in an aqueous medium with a haloperoxidase, a halide source and a hydrogen peroxide source.
Description
TREATMENT OF FABRICS, GARMENTS, OR YARNS WITH SALOPEROXIDASE
FIELD OF THE INVENTION
The present invention relates to a new method of treating undyed fabrics, garments, or yarn comprising treating the undyed fabric, garment, or yarn in an aqueous medium with a haloperoxidase, a halide source and a hydrogen peroxide source.
BACKGROUND OF THE INVENTION
Textiles composed of materials such as wool and, in particular, cellulosics such as cotton, are frequently bleached during manufacturing. Hydrogen peroxide is often used as a bleaching agent. In addition to hydrogen peroxide, the bleaching solutions will normally contain silicates, caustic agents, chelators, organic stabilizers, magnesium salts, and wetting agents. The bleaching treatment has two primary functions; the first is to obtain a high level of whiteness, and the second (when the textile is a cellulosic material) is to break down and solubilize mote materials. Typical bleaching conditions are 0.5-1.5% hydrogen peroxide, 0.5-2o sodium silicate, 0.1-0.4o caustic, and 0.2o chelators at a temperature of 100°C. WO 92/18683 describes a process for bleaching dyed textiles with peroxidases and oxidases.
Furthermore, fabrics, garments, or yarns are sometimes treated in order to improve dyeing characteristics such as dye uptake.
Furthermore, fabrics, garments, or yarns of wool or other animal hair fibers are sometimes treated in order to protect 3o against the tendency to shrink. Methods to generate shrink-resistant fabrics, garments, or yarns are known. The most commonly used method for wool is the IWS/CSIRO Chlorine Hercosett process, which comprises an acid chlorination of wool, followed by a polymer application. This process imparts a high degree of shrink-resistance to wool, but adversely affects the handle of wool, and generates environmentally damaging waste. Other methods to reduce shrinkage of fabrics, garments, or yarns which do not result in release of damaging _.......__._._ __ . _..._. _ ___~ ____._ _~_4_.__
FIELD OF THE INVENTION
The present invention relates to a new method of treating undyed fabrics, garments, or yarn comprising treating the undyed fabric, garment, or yarn in an aqueous medium with a haloperoxidase, a halide source and a hydrogen peroxide source.
BACKGROUND OF THE INVENTION
Textiles composed of materials such as wool and, in particular, cellulosics such as cotton, are frequently bleached during manufacturing. Hydrogen peroxide is often used as a bleaching agent. In addition to hydrogen peroxide, the bleaching solutions will normally contain silicates, caustic agents, chelators, organic stabilizers, magnesium salts, and wetting agents. The bleaching treatment has two primary functions; the first is to obtain a high level of whiteness, and the second (when the textile is a cellulosic material) is to break down and solubilize mote materials. Typical bleaching conditions are 0.5-1.5% hydrogen peroxide, 0.5-2o sodium silicate, 0.1-0.4o caustic, and 0.2o chelators at a temperature of 100°C. WO 92/18683 describes a process for bleaching dyed textiles with peroxidases and oxidases.
Furthermore, fabrics, garments, or yarns are sometimes treated in order to improve dyeing characteristics such as dye uptake.
Furthermore, fabrics, garments, or yarns of wool or other animal hair fibers are sometimes treated in order to protect 3o against the tendency to shrink. Methods to generate shrink-resistant fabrics, garments, or yarns are known. The most commonly used method for wool is the IWS/CSIRO Chlorine Hercosett process, which comprises an acid chlorination of wool, followed by a polymer application. This process imparts a high degree of shrink-resistance to wool, but adversely affects the handle of wool, and generates environmentally damaging waste. Other methods to reduce shrinkage of fabrics, garments, or yarns which do not result in release of damaging _.......__._._ __ . _..._. _ ___~ ____._ _~_4_.__
2 substances to the environment have been described, including processes such as low-temperature plasma treatments.
gU~ARY OF THE INVENTION
The object of the present invention is to provide an enzyme-based method for treating fabrics, garments, or yarn, in order to provide advantages with regard to improved bleaching effect, dye uptake, and/or shrink-resistance, and by which methods, it is possible to reduce fiber damage and limit 1o the use of environmentally damaging chemicals.
It has now been found that certain properties of fabrics, garments, or yarn may be improved by subjecting the undyed fabric, garment, or yarn to a treatment with a haloperoxidase together with a hydrogen peroxide source and a halide source 15 in an amount effective for providing the desired effect.
One embodiment of the invention provides a method of manufacturing a bleached fabric, garment or yarn comprising treating undyed fabric, garment or yarn in an aqueous medium with an effective amount of a haloperoxidase, a halide source 2o and a hydrogen peroxide source at a lower temperature typically at 30-70°C than what is used in a traditional hydrogen peroxide bleaching. This embodiment provides a process for bleaching undyed fabric, garment or yarn at a lower temperature than 100°C, and a bleaching process which 25 requires less chemicals than what is needed today. Another embodiment provides a method of bleaching motes in a cellulosic fabric, garment or yarn comprising treating undyed fabric, garment or yarn in an aqueous medium with an effective amount of a haloperoxidase, a halide source and a hydrogen 30 peroxide source.
Another embodiment of the invention provides a method of manufacturing fabrics, garme..ts, or yarns with improved shrink-resistance or dye uptake. The fabric, garment, or yarn is preferably of wool.
35 Other aspects of the invention will become apparent from the following detailed description and the claims.
gU~ARY OF THE INVENTION
The object of the present invention is to provide an enzyme-based method for treating fabrics, garments, or yarn, in order to provide advantages with regard to improved bleaching effect, dye uptake, and/or shrink-resistance, and by which methods, it is possible to reduce fiber damage and limit 1o the use of environmentally damaging chemicals.
It has now been found that certain properties of fabrics, garments, or yarn may be improved by subjecting the undyed fabric, garment, or yarn to a treatment with a haloperoxidase together with a hydrogen peroxide source and a halide source 15 in an amount effective for providing the desired effect.
One embodiment of the invention provides a method of manufacturing a bleached fabric, garment or yarn comprising treating undyed fabric, garment or yarn in an aqueous medium with an effective amount of a haloperoxidase, a halide source 2o and a hydrogen peroxide source at a lower temperature typically at 30-70°C than what is used in a traditional hydrogen peroxide bleaching. This embodiment provides a process for bleaching undyed fabric, garment or yarn at a lower temperature than 100°C, and a bleaching process which 25 requires less chemicals than what is needed today. Another embodiment provides a method of bleaching motes in a cellulosic fabric, garment or yarn comprising treating undyed fabric, garment or yarn in an aqueous medium with an effective amount of a haloperoxidase, a halide source and a hydrogen 30 peroxide source.
Another embodiment of the invention provides a method of manufacturing fabrics, garme..ts, or yarns with improved shrink-resistance or dye uptake. The fabric, garment, or yarn is preferably of wool.
35 Other aspects of the invention will become apparent from the following detailed description and the claims.
3 DETAILED DESCRIPTION OF THE INVENTION
Before the methods of the invention are described, it is to be understood that this invention is not limited to the particular methods described. The terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting since the scope of the present invention will be limited only by the appended claims.
As used in this specification and the appended claims, to the singular forms "a", "an", and "the" include plural references unless the context clearly dictates otherwise.
Thus, for example, references to "haloperoxidase" or "haloperoxidase preparation" include mixtures of such haloperoxidase, reference to "the method" includes one or more methods, and/or steps of the type described herein and/or which will become apparent to those persons skilled in the art upon reading this disclosure and so forth.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood 2o by one of ordinary skill in the art to which the invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are now described. All publications mentioned herein are incorporated herein by reference for the purpose of disclosing and describing the material for which the reference was cited in connection with.
The term "undyed" refers to fabric, garment, or yarn that has not fully completed a dyeing process. Dyeing may optionally be carried out during or after the method according to the invention. Preferably the enzyme treatment is carried out before the dyeing step.
The term "bleaching" is here defined as a whitening of the fabric, garment, or yarn, and can be measured by using the change in the color space coordinates L*a*b* (CIELAB-system): L*
gives the change in white/black at a scale of from 0 to 100. A
decrease in L* means an increase in black colour (decrease of white colour), an increase in L* means an increase in white _...... ..._........_.w.w v~._...__....._ -,.,-, .._.. _._._ _...... ..._ ....~._.~,_..~...r._...,... .
Before the methods of the invention are described, it is to be understood that this invention is not limited to the particular methods described. The terminology used herein is for the purpose of describing particular embodiments only, and is not intended to be limiting since the scope of the present invention will be limited only by the appended claims.
As used in this specification and the appended claims, to the singular forms "a", "an", and "the" include plural references unless the context clearly dictates otherwise.
Thus, for example, references to "haloperoxidase" or "haloperoxidase preparation" include mixtures of such haloperoxidase, reference to "the method" includes one or more methods, and/or steps of the type described herein and/or which will become apparent to those persons skilled in the art upon reading this disclosure and so forth.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood 2o by one of ordinary skill in the art to which the invention belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are now described. All publications mentioned herein are incorporated herein by reference for the purpose of disclosing and describing the material for which the reference was cited in connection with.
The term "undyed" refers to fabric, garment, or yarn that has not fully completed a dyeing process. Dyeing may optionally be carried out during or after the method according to the invention. Preferably the enzyme treatment is carried out before the dyeing step.
The term "bleaching" is here defined as a whitening of the fabric, garment, or yarn, and can be measured by using the change in the color space coordinates L*a*b* (CIELAB-system): L*
gives the change in white/black at a scale of from 0 to 100. A
decrease in L* means an increase in black colour (decrease of white colour), an increase in L* means an increase in white _...... ..._........_.w.w v~._...__....._ -,.,-, .._.. _._._ _...... ..._ ....~._.~,_..~...r._...,... .
4 colour (a decrease in black colour). Bleaching may also be measured using Stensby units (W = L + 3a - 3b).
Fabric can be constructed from fibres by weaving, knitting or non-woven operations. Weaving and knitting require yarn as the input whereas a non-woven fabric is the result of random bonding of fibres (paper can be thought of as non-woven).
Woven fabric is constructed by weaving "filling" or weft yarns between wrap yarns stretched in the longitudinal direction on the loom. The wrap yarns must be sized before weaving in order to lubricate and protect them from abrasion at the high speed insertion of the filling yarns during weaving. The filling yarn can be woven through the warp yarns in a "over one - under the next" fashion (plain weave) or by "over one - under two" (twill) or any other myriad of permutations. Strength, texture and pattern are related not only to the type/quality of the yarn but also the type of weave. Generally, dresses, shirts, pants, sheeting's, towels, draperies, etc. are produced from woven fabric.
2o Knitting is forming a fabric by joining together interlocking loops of yarn. As opposed to weaving which is constructed from two types of yarn and has many "ends", knitted fabric is produced from a single continuous strand of yarn. As with weaving, there are many different ways to loop yarn together and the final fabric properties are dependent both upon the yarn and the type of knit. Underwear, sweaters, socks, sport shirts, sweat shirts, etc. are derived from knit fabrics.
Non-woven fabrics are sheets of fabric made by bonding 3o and/or interlocking fibres and filaments by mechanical, thermal, chemical or solvent-mediated processes. The resultant fabric can be in the form of web-like structures, laminates or films. Typical examples are disposable baby diapers, towels, wipes, surgical gowns, garments for the "environmental friendly" fashion, filter media, bedding, roofing materials, backing for two-dimensional fabrics and many others.
According to the invention, the process may be applied to any fabric known in the art (woven, knitted, or non-woven). In t ___._. __.____.~._______ ________..._.~..~_w____... _..._..._-~._~._.._ particular the bleaching process may be applied to cellulose-containing or cellulosic fabrics, such as cotton, viscose, rayon, ramie, linen, lyocell (e.g., Tencel, produced by Courtaulds Fibers), or mixtures thereof, or mixtures of any of
Fabric can be constructed from fibres by weaving, knitting or non-woven operations. Weaving and knitting require yarn as the input whereas a non-woven fabric is the result of random bonding of fibres (paper can be thought of as non-woven).
Woven fabric is constructed by weaving "filling" or weft yarns between wrap yarns stretched in the longitudinal direction on the loom. The wrap yarns must be sized before weaving in order to lubricate and protect them from abrasion at the high speed insertion of the filling yarns during weaving. The filling yarn can be woven through the warp yarns in a "over one - under the next" fashion (plain weave) or by "over one - under two" (twill) or any other myriad of permutations. Strength, texture and pattern are related not only to the type/quality of the yarn but also the type of weave. Generally, dresses, shirts, pants, sheeting's, towels, draperies, etc. are produced from woven fabric.
2o Knitting is forming a fabric by joining together interlocking loops of yarn. As opposed to weaving which is constructed from two types of yarn and has many "ends", knitted fabric is produced from a single continuous strand of yarn. As with weaving, there are many different ways to loop yarn together and the final fabric properties are dependent both upon the yarn and the type of knit. Underwear, sweaters, socks, sport shirts, sweat shirts, etc. are derived from knit fabrics.
Non-woven fabrics are sheets of fabric made by bonding 3o and/or interlocking fibres and filaments by mechanical, thermal, chemical or solvent-mediated processes. The resultant fabric can be in the form of web-like structures, laminates or films. Typical examples are disposable baby diapers, towels, wipes, surgical gowns, garments for the "environmental friendly" fashion, filter media, bedding, roofing materials, backing for two-dimensional fabrics and many others.
According to the invention, the process may be applied to any fabric known in the art (woven, knitted, or non-woven). In t ___._. __.____.~._______ ________..._.~..~_w____... _..._..._-~._~._.._ particular the bleaching process may be applied to cellulose-containing or cellulosic fabrics, such as cotton, viscose, rayon, ramie, linen, lyocell (e.g., Tencel, produced by Courtaulds Fibers), or mixtures thereof, or mixtures of any of
5 these fibres, or mixtures of any of these fibres together with synthetic fibres (e. g., polyester, polyamide, nylon) or other natural fibers such as wool and silk. The term "wool" includes any commercially useful animal hair product, for example, wool from sheep, camel, rabbit, goat, or llamas, and includes wool 1o fiber and animal hair. The method of the invention can be used with wool or animal hair material in the form of top, fiber, yarn, or woven or knitted fabric. The enzymatic treatment can also be carried out on loose flock or on garments made from wool or animal hair material.
The treatment can be performed at many different stages of processing.
~he term "shrinkage" refers to the felting shrinkage of fibers as defined in IWS TM 31, i.e., felting shrinkage is the irreversible shrinkage caused by progressive entanglement of 2o the wool fibers induced by washing in an aqueous solution, and is defined as the reduction in length and/or width induced by washing. Shrinkage can be measured in accordance with IWS TM
31, or it can be measured using the following modification.
Wool samples (24 cm x 24 cm) are sewed around the edges and inscribed with a rectangle (18 cm x 18 cm). Samples are treated, air-dried, then subjected to five cycles of machine washing and drying (warm wash, high heat of drying) in combination with external ballast such as towels and articles of clothing. The dimensions of the rectangle are measured after five cycles, and the shrinkage is defined as the change in dimensions of the rectangle, after accounting for initial relax<~tion shrinkage.
The term "shrink-resistance" is a measure of the reduction in shrinkage (as defined above, after wash/dry cycles) for material that has been treated relative to material that has not been treated, i.e., ................_ _.....~____~-_~__ __..._..__..._ _ .___.~ ...
The treatment can be performed at many different stages of processing.
~he term "shrinkage" refers to the felting shrinkage of fibers as defined in IWS TM 31, i.e., felting shrinkage is the irreversible shrinkage caused by progressive entanglement of 2o the wool fibers induced by washing in an aqueous solution, and is defined as the reduction in length and/or width induced by washing. Shrinkage can be measured in accordance with IWS TM
31, or it can be measured using the following modification.
Wool samples (24 cm x 24 cm) are sewed around the edges and inscribed with a rectangle (18 cm x 18 cm). Samples are treated, air-dried, then subjected to five cycles of machine washing and drying (warm wash, high heat of drying) in combination with external ballast such as towels and articles of clothing. The dimensions of the rectangle are measured after five cycles, and the shrinkage is defined as the change in dimensions of the rectangle, after accounting for initial relax<~tion shrinkage.
The term "shrink-resistance" is a measure of the reduction in shrinkage (as defined above, after wash/dry cycles) for material that has been treated relative to material that has not been treated, i.e., ................_ _.....~____~-_~__ __..._..__..._ _ .___.~ ...
6 PCT/DK98/00242 Shrink-resistance= ( Shrinkage"ntrearea -Shrlrikagetreated) /Shririkagetreated The value is multiplied by 100 in order to be expressed as a percentage.
The term "dye uptake" refers to properties associated with dyeing of fabrics, garments or yarn such as of wool or animal hair material. Dye uptake is a measure of the capacity of wool or animal hair material immersed in a dye solution to to absorb available dyestuff. This property can be measured by the following test. In a suitable reaction vessel, wool or animal hair material is added to a buffered solution of acid black 172 (300 ml of 0.05 M NaOAc buffer, pH 4.5, plus 7.5 mL
of a 1.0% w/w solution of acid black 172 in water). The vessel is incubated in a shaking water bath at 50°C for 15 minutes with mild agitation. After removal of the material from solution, it is allowed to air-dry, then measured in a suitable spectrophotometer to determine CIELAB values. Dye uptake is determined by the L* reading, and changes in dye uptake are found by determining dL* relative to untreated material.
"Mote" particles are dark brown particles found on unbleached cotton fabric, also called "dark spots". They are cotton pod and stem residues originating from the mechanical picking of cotton. The brown colour is due to the high lignin content of the mote particles.
Haloperoxidases In the context of the present invention, the term "haloper-oxidase" is intended to mean an enzyme selected from the group consisting of chloride peroxidase (EC 1.11.1.10), bromide peroxidas=, and iodide peroxidase (EC 1.11.1.8).
A chloride peroxidase is an enzyme capable of oxidizing chloride, bromide and iodide ions with the consumption of H202 .
A bromide peroxidase is an enzyme capable of oxidizing bromide and iodide ions with the consumption of H202.
A iodide peroxidase is an enzyme capable of oxidizing iodide ions with the consumption of H20~.
_... _~.__._._____~._~.._.-._....-.d _..~_._.. _ _......_ .~
The term "dye uptake" refers to properties associated with dyeing of fabrics, garments or yarn such as of wool or animal hair material. Dye uptake is a measure of the capacity of wool or animal hair material immersed in a dye solution to to absorb available dyestuff. This property can be measured by the following test. In a suitable reaction vessel, wool or animal hair material is added to a buffered solution of acid black 172 (300 ml of 0.05 M NaOAc buffer, pH 4.5, plus 7.5 mL
of a 1.0% w/w solution of acid black 172 in water). The vessel is incubated in a shaking water bath at 50°C for 15 minutes with mild agitation. After removal of the material from solution, it is allowed to air-dry, then measured in a suitable spectrophotometer to determine CIELAB values. Dye uptake is determined by the L* reading, and changes in dye uptake are found by determining dL* relative to untreated material.
"Mote" particles are dark brown particles found on unbleached cotton fabric, also called "dark spots". They are cotton pod and stem residues originating from the mechanical picking of cotton. The brown colour is due to the high lignin content of the mote particles.
Haloperoxidases In the context of the present invention, the term "haloper-oxidase" is intended to mean an enzyme selected from the group consisting of chloride peroxidase (EC 1.11.1.10), bromide peroxidas=, and iodide peroxidase (EC 1.11.1.8).
A chloride peroxidase is an enzyme capable of oxidizing chloride, bromide and iodide ions with the consumption of H202 .
A bromide peroxidase is an enzyme capable of oxidizing bromide and iodide ions with the consumption of H202.
A iodide peroxidase is an enzyme capable of oxidizing iodide ions with the consumption of H20~.
_... _~.__._._____~._~.._.-._....-.d _..~_._.. _ _......_ .~
7 Haloperoxidases form a class of enzymes capable of oxidizing halides (X = C1-, Br-, or I-) in the presence of hydrogen peroxide to the corresponding hypohalous acid (HOX) according to the equation:
H20z + X- + H+ -> H20 + HOX
If an appropriate nucleophile is present, a reaction will occur with HOX, whereby bleaching may take place.
to Haloperoxidases have been isolated from various organisms:
mammals, marine animals, plants, algae, a lichen, fungi and bacteria (for reference see Biochimica et Biophysica Acta 1161, 1993, pp. 249-256). It is generally accepted that haloperoxidases are the enzymes responsible for the formation of halogenated compounds in nature, although other enzymes may be involved.
Haloperoxidases have been isolated from many different fungi, in particular from the fungus group dematiaceous hypho-mycetes, such as Caldariomyces, e.g., C. fumago, Alternaria, Curvularia, e.g., C. verruculosa and C. inaequalis, Drechslera, Ulocladium and Botrytis (see US Patent No. 4,937,192).
According to the present invention, a haloperoxidase obtainable from Curvularia, in particular C. verruculosa, is preferred. Curvularia haloperoxidase and recombinant production thereof is described in WO 97/04102.
Haloperoxidase has also been isolated from bacteria such as Pseudomonas, e.g., P. pyrrocinia (for reference see The Journal of Biological Chemistry 263, 1988, pp. 13725-13732) and Streptomyces, e.g., S. aureofaciens (for reference see Struc-3o tural Biology 1, 1994, pp. 532-537).
Bromide peroxidase has been isolated from algae (see US
Patent No. 4,937,192).
In use, the concentration of the haloperoxidase may be varied in order to achieve the desired bleaching effect in the desired time frame. However, according to the invention, the haloperoxidase will normally be added in a concentration of 0.01-100 mg enzyme protein per liter, preferably in a concen-tration of 0.1-50 mg enzyme protein per liter, more preferably
H20z + X- + H+ -> H20 + HOX
If an appropriate nucleophile is present, a reaction will occur with HOX, whereby bleaching may take place.
to Haloperoxidases have been isolated from various organisms:
mammals, marine animals, plants, algae, a lichen, fungi and bacteria (for reference see Biochimica et Biophysica Acta 1161, 1993, pp. 249-256). It is generally accepted that haloperoxidases are the enzymes responsible for the formation of halogenated compounds in nature, although other enzymes may be involved.
Haloperoxidases have been isolated from many different fungi, in particular from the fungus group dematiaceous hypho-mycetes, such as Caldariomyces, e.g., C. fumago, Alternaria, Curvularia, e.g., C. verruculosa and C. inaequalis, Drechslera, Ulocladium and Botrytis (see US Patent No. 4,937,192).
According to the present invention, a haloperoxidase obtainable from Curvularia, in particular C. verruculosa, is preferred. Curvularia haloperoxidase and recombinant production thereof is described in WO 97/04102.
Haloperoxidase has also been isolated from bacteria such as Pseudomonas, e.g., P. pyrrocinia (for reference see The Journal of Biological Chemistry 263, 1988, pp. 13725-13732) and Streptomyces, e.g., S. aureofaciens (for reference see Struc-3o tural Biology 1, 1994, pp. 532-537).
Bromide peroxidase has been isolated from algae (see US
Patent No. 4,937,192).
In use, the concentration of the haloperoxidase may be varied in order to achieve the desired bleaching effect in the desired time frame. However, according to the invention, the haloperoxidase will normally be added in a concentration of 0.01-100 mg enzyme protein per liter, preferably in a concen-tration of 0.1-50 mg enzyme protein per liter, more preferably
8 in a concentration of 1-10 mg enzyme protein per liter.
Halide Sources According to the invention, the halide source for the reaction with haloperoxidase may be achieved in many different ways: The halide source may be sodium chloride, potassium chloride, sodium bromide, potassium bromide, sodium iodide, or potassium iodide. The concentration of the halide source will typically correspond to 0.01-1000 mM, preferably in the range of from 0.1-500 mM.
Hydrogen Peroxide Sources According to the invention, the hydrogen peroxide needed for the reaction with the haloperoxidase may be achieved in many different ways: It may be hydrogen peroxide or a hydrogen peroxide precursor, such as percarbonate or perborate, or a peroxycarboxylic acid or a salt thereof, or it may be a hydrogen peroxide generating enzyme system, such as an oxidase and its substrate. Useful oxidases include glucose oxidase, a glycerol oxidase or an amino acid oxidase. An example of an amino acid oxidase is given in WO 99/25574.
2o According to the invention, the hydrogen peroxide source needed for the reaction with the haloperoxidase may be added in a concentration corresponding to a hydrogen peroxide concentration in the range of from 0.01-1000 mM, preferably in the range of from 0.1-500 mM.
Process The chosen procedure will depend on the haloperoxidase in question, regarding pH optimum, temperature optimum, etc.
If a haloperoxidase from Curvularia verruculosa is used, the processing conditions could be:
30-70°C, pH 5, using 1-5 mg enzyme/liter, 50-500 mM halide (e.g. sodium chloride), 20 mM hydrogen peroxide, at a liquor/fabric ratio of from 4:1-30:1, for a reac'W_on time of 30-120 min. (as illustrated in Example 1).
A buffer may be added to the reaction medium to maintain a suitable pH for the haloperoxidase used. The buffer may suitably be a phosphate, borate, citrate, acetate, adipate, triethanolamine, monoethanolamine, diethanolamine, carbonate (especially alkali metal or alkaline earth metal, in par-..~....__ ~.. . _ ___.
Halide Sources According to the invention, the halide source for the reaction with haloperoxidase may be achieved in many different ways: The halide source may be sodium chloride, potassium chloride, sodium bromide, potassium bromide, sodium iodide, or potassium iodide. The concentration of the halide source will typically correspond to 0.01-1000 mM, preferably in the range of from 0.1-500 mM.
Hydrogen Peroxide Sources According to the invention, the hydrogen peroxide needed for the reaction with the haloperoxidase may be achieved in many different ways: It may be hydrogen peroxide or a hydrogen peroxide precursor, such as percarbonate or perborate, or a peroxycarboxylic acid or a salt thereof, or it may be a hydrogen peroxide generating enzyme system, such as an oxidase and its substrate. Useful oxidases include glucose oxidase, a glycerol oxidase or an amino acid oxidase. An example of an amino acid oxidase is given in WO 99/25574.
2o According to the invention, the hydrogen peroxide source needed for the reaction with the haloperoxidase may be added in a concentration corresponding to a hydrogen peroxide concentration in the range of from 0.01-1000 mM, preferably in the range of from 0.1-500 mM.
Process The chosen procedure will depend on the haloperoxidase in question, regarding pH optimum, temperature optimum, etc.
If a haloperoxidase from Curvularia verruculosa is used, the processing conditions could be:
30-70°C, pH 5, using 1-5 mg enzyme/liter, 50-500 mM halide (e.g. sodium chloride), 20 mM hydrogen peroxide, at a liquor/fabric ratio of from 4:1-30:1, for a reac'W_on time of 30-120 min. (as illustrated in Example 1).
A buffer may be added to the reaction medium to maintain a suitable pH for the haloperoxidase used. The buffer may suitably be a phosphate, borate, citrate, acetate, adipate, triethanolamine, monoethanolamine, diethanolamine, carbonate (especially alkali metal or alkaline earth metal, in par-..~....__ ~.. . _ ___.
9 ticular sodium or potassium carbonate, or ammonium and HC1 salts), diamine, especially diaminoethane, imidazole, or amino acid buffer.
The process of the invention may be carried out in the presence of conventional fabric, garment, or yarn finishing agents, including wetting agents, polymeric agents, dispersing agents, etc.
A conventional wetting agent may be used to improve the contact between the substrate and the enzyme used in the l0 process. The wetting agent may be a nonionic surfactant, e.g., an ethoxylated fatty alcohol. A very useful wetting agent is an ethoxylated and propoxylated fatty acid ester such as Berol 087 (product of Akzo Nobel, Sweden).
Bx<smples of suitable polymers include proteins (e. g., bovine serum albumin, whey, casein or legume proteins), protein hydrolysates (e. g., whey, casein or soy protein hydrolysate), polypeptides, lignosulfonates, polysaccharides and derivatives thereof, polyethylene glycol, polypropylene glycol, polyvinyl pyrrolidone, ethylene diamine condensed with ethylene or propylene oxide, ethoxylated polyamines, or ethoxylated amine polymers.
The dispersing agent may suitably be selected from nonionic, anionic, cationic, ampholytic or zwitterionic surfactants. More specifically, the dispersing agent may be selected from carboxymethylcellulose, hydroxypropylcellulose, alkyl aryl sulphonates, long-chain alcohol sulphates (primary and secondary alkyl sulphates), sulphonated olefins, sulphated monoglycerides, sulphated ethers, sulphosuccinates, sulphonated methyl ethers, alkane sulphonates, phosphate esters, alkyl isothionates, acylsarcosides, alkyltaurides, fluorosurfactants, fatty alcohol and alkylphenol condensates, fatty acid condensates, condensates of ethylene oxide with an amine, condensates of ethylene oxide with an amide, sucrose esters, sorbitan esters, alkyloamides, fatty amine oxides, ethoxylated monoamines, ethoxylated diamines, alcohol ethoxylate and mixtures thereof. A very useful dispersing agent is an alcohol ethoxylate such as Berol 08 (product of Akzo Nobel, Sweden).
The bleaching processing may be performed in any machinery known in the art.
Inactivation of the haloperoxidase in question will normally not be necessary; however if an inactivation of the 5 enzyme is wanted it may be performed as known in the art, e.g., high temperature and/or high pH, but the specific inactivation conditions will of course depend on the enzyme in use.
The fabric may be further finished by one or more of the
The process of the invention may be carried out in the presence of conventional fabric, garment, or yarn finishing agents, including wetting agents, polymeric agents, dispersing agents, etc.
A conventional wetting agent may be used to improve the contact between the substrate and the enzyme used in the l0 process. The wetting agent may be a nonionic surfactant, e.g., an ethoxylated fatty alcohol. A very useful wetting agent is an ethoxylated and propoxylated fatty acid ester such as Berol 087 (product of Akzo Nobel, Sweden).
Bx<smples of suitable polymers include proteins (e. g., bovine serum albumin, whey, casein or legume proteins), protein hydrolysates (e. g., whey, casein or soy protein hydrolysate), polypeptides, lignosulfonates, polysaccharides and derivatives thereof, polyethylene glycol, polypropylene glycol, polyvinyl pyrrolidone, ethylene diamine condensed with ethylene or propylene oxide, ethoxylated polyamines, or ethoxylated amine polymers.
The dispersing agent may suitably be selected from nonionic, anionic, cationic, ampholytic or zwitterionic surfactants. More specifically, the dispersing agent may be selected from carboxymethylcellulose, hydroxypropylcellulose, alkyl aryl sulphonates, long-chain alcohol sulphates (primary and secondary alkyl sulphates), sulphonated olefins, sulphated monoglycerides, sulphated ethers, sulphosuccinates, sulphonated methyl ethers, alkane sulphonates, phosphate esters, alkyl isothionates, acylsarcosides, alkyltaurides, fluorosurfactants, fatty alcohol and alkylphenol condensates, fatty acid condensates, condensates of ethylene oxide with an amine, condensates of ethylene oxide with an amide, sucrose esters, sorbitan esters, alkyloamides, fatty amine oxides, ethoxylated monoamines, ethoxylated diamines, alcohol ethoxylate and mixtures thereof. A very useful dispersing agent is an alcohol ethoxylate such as Berol 08 (product of Akzo Nobel, Sweden).
The bleaching processing may be performed in any machinery known in the art.
Inactivation of the haloperoxidase in question will normally not be necessary; however if an inactivation of the 5 enzyme is wanted it may be performed as known in the art, e.g., high temperature and/or high pH, but the specific inactivation conditions will of course depend on the enzyme in use.
The fabric may be further finished by one or more of the
10 following treatments as known in the art: dyeing, biopolishing, brightening, softening, and/or anti-wrinkling treatment(s).
Test Procedure The test procedure for fabric bleaching may be performed visually and by using a Minolta Chroma Meter CR200, a Minolta Chroma Meter CR300 or a Minolta Chroma Meter 5081.
Evaluation: A Minolta Chroma Meter (available from Minolta Corp.) is used according to Manufacturer's instructions to evaluate the degree of bleaching as well as to estimate any 2o discoloration using the change in the colour space coordinates L*a*b* (CIELAB-system) : L* gives the change in white/black at a scale of from 0 to 100, a* gives the change in green (-a*)/red (+a*) , and b* gives the change in blue (-b*) /yellow (+b*) . A
decrease in L* means an increase in black colour (decrease of white colour), an increase in L* means an increase in white colour (a decrease in black colour), a decrease in a means an increase in green colour (decrease in red colour), an increase in a* means an increase in red colour (a decrease in green colour), a decrease in b* means an increase in blue colour (a 3o decrease in yellow colour), and an increase in b* means an increase in yellow col~ ~Y (a decrease in blue colour).
The instrument is c .~.brated using a standard calibration plate (white).
The invention is further illustrated in the following examples, which are not intended to be in any way limiting to the scope of the invention as claimed.
_r ........ _.~~.___.__.... _.__-__.._..T.
Test Procedure The test procedure for fabric bleaching may be performed visually and by using a Minolta Chroma Meter CR200, a Minolta Chroma Meter CR300 or a Minolta Chroma Meter 5081.
Evaluation: A Minolta Chroma Meter (available from Minolta Corp.) is used according to Manufacturer's instructions to evaluate the degree of bleaching as well as to estimate any 2o discoloration using the change in the colour space coordinates L*a*b* (CIELAB-system) : L* gives the change in white/black at a scale of from 0 to 100, a* gives the change in green (-a*)/red (+a*) , and b* gives the change in blue (-b*) /yellow (+b*) . A
decrease in L* means an increase in black colour (decrease of white colour), an increase in L* means an increase in white colour (a decrease in black colour), a decrease in a means an increase in green colour (decrease in red colour), an increase in a* means an increase in red colour (a decrease in green colour), a decrease in b* means an increase in blue colour (a 3o decrease in yellow colour), and an increase in b* means an increase in yellow col~ ~Y (a decrease in blue colour).
The instrument is c .~.brated using a standard calibration plate (white).
The invention is further illustrated in the following examples, which are not intended to be in any way limiting to the scope of the invention as claimed.
_r ........ _.~~.___.__.... _.__-__.._..T.
11 Bleaching of raw cotton swatches with Curvularia verruculosa haloperoxidase Experimental conditions:
The bleaching system contained 3 mg/1 recombinant Curvularia verruculosa haloperoxidase with [NaCl]=100 mM as substrate and [H202]=20 mM as donor. pH was adjusted to pH=5.
The swatches were bleached for 60 minutes at 40°C. (The enzyme was produced as described in WO 97/04102).
The bleaching system was tested on twill cotton swatches and woven cotton swatches.
For twill fabric the fabric/liquor ratio was: 1 g of fabric in 15 ml of aqueous medium.
For woven fabric the fabric/liquor ratio was: 1 g of fabric in 20 ml of aqueous medium.
Results:
2o Significant visual bleaching was obtained with the experimental conditions described above.
Note that the blind test assures that the observed bleaching effect is enzymatic in nature.
The bleaching results are presented in the Table 1 below:
__._ ~..__~.~_~______.~.-~__._.
The bleaching system contained 3 mg/1 recombinant Curvularia verruculosa haloperoxidase with [NaCl]=100 mM as substrate and [H202]=20 mM as donor. pH was adjusted to pH=5.
The swatches were bleached for 60 minutes at 40°C. (The enzyme was produced as described in WO 97/04102).
The bleaching system was tested on twill cotton swatches and woven cotton swatches.
For twill fabric the fabric/liquor ratio was: 1 g of fabric in 15 ml of aqueous medium.
For woven fabric the fabric/liquor ratio was: 1 g of fabric in 20 ml of aqueous medium.
Results:
2o Significant visual bleaching was obtained with the experimental conditions described above.
Note that the blind test assures that the observed bleaching effect is enzymatic in nature.
The bleaching results are presented in the Table 1 below:
__._ ~..__~.~_~______.~.-~__._.
12 ~L*/Da*/Ob* on raw cotton swatchesa.
Bleaching Twillb Wovenb system Blind (-)0.2 / 0.1 / 0.0 (-)0.2 / 0.0 / (-)0.1 Enzymes 2.5 / (-)0.9 / (-)1.5 1.6 / (-)0.6 / (-)1.3 a: Measurements were all done on a Minolta 5081.
Lamp was set to D65 and 2°.
b: Desized swatches obtained from Test Fabrics.
~: System consists of NaCl, hydrogen peroxide and acetate buffer.
s: System consists of haloperoxidase, NaCl, hydrogen peroxide and acetate buffer.
to EXAMPhE 2 Bleaching of motes with Curvularia verruculosa haloperoxidase Experimental conditions:
The bleaching system was the same as described in Example 1: 3 mg/1 recombinant Curvularia verruculosa haloperoxidase with [NaCl]=100 mM as substrate and [Hz02]=20 mM as donor. pH
was adjusted to pH=5.
2o The swatches were bleached for 60 minutes at 90°C in an Atlas LP2 Lauder-o-meter. Linen woven 1000 cotton was supplied by Nordisk Textil V~veri & Trykkeri A/S. The fabric/liquor ratio was 1 g of fabric in 20 ml of aqueous medium.
Results Motes were counted on a fabric area of 10 cm x 15 cm (on both sides).
A mote was defined as a "dark spot" on the cotton surface irrespective of size.
3o Double determination of the mote bleaching effect was carried out.
The numbers 1 and 2 in Table 2 refer to the separate fabric cloths used.
?. __.__..~..___..T ______... .L
Bleaching Twillb Wovenb system Blind (-)0.2 / 0.1 / 0.0 (-)0.2 / 0.0 / (-)0.1 Enzymes 2.5 / (-)0.9 / (-)1.5 1.6 / (-)0.6 / (-)1.3 a: Measurements were all done on a Minolta 5081.
Lamp was set to D65 and 2°.
b: Desized swatches obtained from Test Fabrics.
~: System consists of NaCl, hydrogen peroxide and acetate buffer.
s: System consists of haloperoxidase, NaCl, hydrogen peroxide and acetate buffer.
to EXAMPhE 2 Bleaching of motes with Curvularia verruculosa haloperoxidase Experimental conditions:
The bleaching system was the same as described in Example 1: 3 mg/1 recombinant Curvularia verruculosa haloperoxidase with [NaCl]=100 mM as substrate and [Hz02]=20 mM as donor. pH
was adjusted to pH=5.
2o The swatches were bleached for 60 minutes at 90°C in an Atlas LP2 Lauder-o-meter. Linen woven 1000 cotton was supplied by Nordisk Textil V~veri & Trykkeri A/S. The fabric/liquor ratio was 1 g of fabric in 20 ml of aqueous medium.
Results Motes were counted on a fabric area of 10 cm x 15 cm (on both sides).
A mote was defined as a "dark spot" on the cotton surface irrespective of size.
3o Double determination of the mote bleaching effect was carried out.
The numbers 1 and 2 in Table 2 refer to the separate fabric cloths used.
?. __.__..~..___..T ______... .L
13 Note that a positive difference in mote count can be due to the motes splitting up due to the mechanical handling of the fabric cloth.
Table 2:
Mote Mote count Difference count before after in mote bleaching bleac hing count Side of Side Side Side Side Side Side2 fabric cloth 1 2 1 2 1 Reference 1 85 74 91 71 - +6 -3 Reference 2a 78 68 69 70 -9 +2 Blind 1~' 60 50 62 52 +2 +2 Blind 2b 72 74 77 75 +5 +1 Enzymatic l~ 53 62 49 42 -4 -20 Enzymatic 2~ 68 62 41 56 -27 -6 a: Fabric washed in buffer only.
b: Conditions as described above in the experimental section 1o but without added enzyme.
~: Conditions as described above in the experimental section.
The reference tests illustrate the effects of the mechanical washing procedure and as can be seen from Table 2, the loss of motes is ambiguous. (The mechanical washing procedure has no significant effect on the number of motes left on the cloth after the bleaching.) Table 2 shows that there is a significant loss of motes when submitting the fabric cloth to the enzymatic bleaching 2o conditions. The blind test assures that the observed effect is enzymatic in nature.
Table 2:
Mote Mote count Difference count before after in mote bleaching bleac hing count Side of Side Side Side Side Side Side2 fabric cloth 1 2 1 2 1 Reference 1 85 74 91 71 - +6 -3 Reference 2a 78 68 69 70 -9 +2 Blind 1~' 60 50 62 52 +2 +2 Blind 2b 72 74 77 75 +5 +1 Enzymatic l~ 53 62 49 42 -4 -20 Enzymatic 2~ 68 62 41 56 -27 -6 a: Fabric washed in buffer only.
b: Conditions as described above in the experimental section 1o but without added enzyme.
~: Conditions as described above in the experimental section.
The reference tests illustrate the effects of the mechanical washing procedure and as can be seen from Table 2, the loss of motes is ambiguous. (The mechanical washing procedure has no significant effect on the number of motes left on the cloth after the bleaching.) Table 2 shows that there is a significant loss of motes when submitting the fabric cloth to the enzymatic bleaching 2o conditions. The blind test assures that the observed effect is enzymatic in nature.
14 Treatment of wool with Curvularia verruculosa haloperoxidase _Experimental conditions:
The enzyme system was the same as described in Example 1:
3 mg/1 recombinant Curvularia verruculosa haloperoxidase with [NaCl]=100 mM as substrate and [Hz02]=20 mM as donor. pH was adjusted to pH=5.
Swatches (24 cm x 24 cm, approx. 10 g each) of TF532 Jersey Knit Wool were cut and sewn around the edge with a Burger. A permanent marker was used to draw an 18 x 18 rectangle on each swatch.
Results:
Treatment Shrinkage ( o) Blind 29 Enzymatic 23 __._.
The enzyme system was the same as described in Example 1:
3 mg/1 recombinant Curvularia verruculosa haloperoxidase with [NaCl]=100 mM as substrate and [Hz02]=20 mM as donor. pH was adjusted to pH=5.
Swatches (24 cm x 24 cm, approx. 10 g each) of TF532 Jersey Knit Wool were cut and sewn around the edge with a Burger. A permanent marker was used to draw an 18 x 18 rectangle on each swatch.
Results:
Treatment Shrinkage ( o) Blind 29 Enzymatic 23 __._.
Claims (23)
1. A method of treating fabrics, garments, or yarns comprising treating undyed fabric, garment, or yarn in an aqueous medium with an effective amount of a haloperoxidase, a halide source, and a hydrogen peroxide source.
2. A method according to claim 1, wherein the fabric, garment, or yarn is a cellulosic fabric.
3. A method according to claim 1, wherein the fabric, garment, or yarn is a silk fabric or a wool fabric.
4. A method according to claim 2, wherein the cellulosic fabric is denim.
5. A method according to claim 1, wherein the haloperoxidase is obtainable from fungi, from bacteria, or from algae.
6. A method according to claim 5, wherein the haloperoxidase is obtainable from a fungus selected from the group consisting of Caldariomyces, Alternaria, Curvularia, Drechslera, Ulocladium and Botrytis.
7. A method according to claim 6, wherein the haloperoxidase is obtainable from Curvularia.
8. A method according to claim 7, wherein the haloperoxidase is obtainable from Curvularia verruculosa.
9. A method according to claim 5, wherein the haloperoxidase is obtainable from a bacterium selected from the group consisting of Pseudomonas and Streptomyces.
10. A method according to claim 1, wherein the concentration of the haloperoxidase is in the range of from 0.01-100 mg enzyme protein per liter.
11. A method according to claim 1, wherein the halide source is sodium chloride, potassium chloride, sodium bromide, potassium bromide, sodium iodide, or potassium iodide.
12. A method according to claim 1, wherein the source of hydrogen peroxide is hydrogen peroxide, or a hydrogen peroxide precursor, e.g., percarbonate or perborate, or a hydrogen peroxide generating enzyme system, e.g., an oxidase and its substrate, or a peroxycarboxylic acid or a salt thereof.
13. A method according to claim 11, wherein the concentration of the halide source corresponds to 0.01-1000 mM.
14. A method according to claim 12, wherein the concentration of the hydrogen peroxide source corresponds to a hydrogen peroxide concentration in the range of from 0.01-1000 mM.
15. A method according to claim 1, wherein the haloperoxidase is applied at a temperature below 70°C.
16. A method according to claim 1, wherein the aqueous medium contains a surfactant.
17. A method of manufacturing a bleached fabric comprising treating undyed fabric by the method of any of claims 1-16.
18. A method of manufacturing a bleached yarn comprising treating undyed yarn by the method of any of claims 1-16.
19. A method of bleaching motes in a fabric comprising treating undyed fabric by the method of any of claims 1-16.
20. A method of any of claims 17-19 wherein the fabric or yarn is of cellulosic material.
21. A method of manufacturing fabrics, garments, or yarns with improved shrink-resistance by the method of any of claims 1-16.
22. A method of manufacturing fabrics, garments, or yarns with improved dye-uptake by the method of any of claims 1-16.
23. A method of any of claims 21-22 wherein the fabric, garment, or yarn is of wool.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK0673/97 | 1997-06-09 | ||
DK67397 | 1997-06-09 | ||
PCT/DK1998/000242 WO1998056976A1 (en) | 1997-06-09 | 1998-06-09 | Treatment of fabrics, garments, or yarns with haloperoxidase |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2293600A1 true CA2293600A1 (en) | 1998-12-17 |
Family
ID=8096234
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA002293600A Abandoned CA2293600A1 (en) | 1997-06-09 | 1998-06-09 | Treatment of fabrics, garments, or yarns with haloperoxidase |
Country Status (7)
Country | Link |
---|---|
JP (1) | JP2002504192A (en) |
AT (1) | ATE336613T1 (en) |
BR (1) | BR9809976A (en) |
CA (1) | CA2293600A1 (en) |
DE (1) | DE69835596D1 (en) |
PL (1) | PL337367A1 (en) |
TR (1) | TR199902983T2 (en) |
-
1998
- 1998-06-09 JP JP50134799A patent/JP2002504192A/en active Pending
- 1998-06-09 AT AT98929223T patent/ATE336613T1/en not_active IP Right Cessation
- 1998-06-09 CA CA002293600A patent/CA2293600A1/en not_active Abandoned
- 1998-06-09 PL PL98337367A patent/PL337367A1/en unknown
- 1998-06-09 TR TR1999/02983T patent/TR199902983T2/en unknown
- 1998-06-09 BR BR9809976-0A patent/BR9809976A/en not_active IP Right Cessation
- 1998-06-09 DE DE69835596T patent/DE69835596D1/en not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
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BR9809976A (en) | 2000-08-01 |
DE69835596D1 (en) | 2006-09-28 |
TR199902983T2 (en) | 2003-03-21 |
ATE336613T1 (en) | 2006-09-15 |
PL337367A1 (en) | 2000-08-14 |
JP2002504192A (en) | 2002-02-05 |
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