CA2261151A1 - Transformation of pichia methanolica - Google Patents

Transformation of pichia methanolica

Info

Publication number
CA2261151A1
CA2261151A1 CA002261151A CA2261151A CA2261151A1 CA 2261151 A1 CA2261151 A1 CA 2261151A1 CA 002261151 A CA002261151 A CA 002261151A CA 2261151 A CA2261151 A CA 2261151A CA 2261151 A1 CA2261151 A1 CA 2261151A1
Authority
CA
Canada
Prior art keywords
methanolica
dna molecules
cells
transformation
pichia methanolica
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002261151A
Other languages
French (fr)
Other versions
CA2261151C (en
Inventor
Christopher K. Raymond
Susan D. Holderman
Erica Vanaja
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zymogenetics Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2261151A1 publication Critical patent/CA2261151A1/en
Application granted granted Critical
Publication of CA2261151C publication Critical patent/CA2261151C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Methods for transforming Pichia methanolica, and DNA molecules useful in transformation of P. methanolica, are disclosed. P. methanolica cells are exposed, in the presence of DNA molecules, to a pulsed electric field having a field strength of from 2.5 to 4.5 kV/cm and a pulse duration of from 1 to 40 milliseconds, whereby the DNA molecules are introduced into the cells. The DNA
molecules may comprise an expression unit that includes a transcription promoter of a P. methanolica gene operably linked to a segment encoding a polypeptide or protein of interest. The DNA molecules may also encode a selectable marker, such as a P. methanolica ADE2 gene. Cells transformed according to the invention may be used in production systems for the preparation of proteins of commercial importance.
CA002261151A 1996-07-17 1997-07-14 Transformation of pichia methanolica Expired - Fee Related CA2261151C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US68350096A 1996-07-17 1996-07-17
US08/683,500 1996-08-26
PCT/US1997/012581 WO1998002565A1 (en) 1996-07-17 1997-07-14 TRANSFORMATION OF $i(PICHIA METHANOLICA)

Publications (2)

Publication Number Publication Date
CA2261151A1 true CA2261151A1 (en) 1998-01-22
CA2261151C CA2261151C (en) 2003-09-16

Family

ID=24744316

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002261151A Expired - Fee Related CA2261151C (en) 1996-07-17 1997-07-14 Transformation of pichia methanolica

Country Status (5)

Country Link
JP (1) JP2002515746A (en)
AU (1) AU718510B2 (en)
CA (1) CA2261151C (en)
IL (1) IL128073A0 (en)
WO (1) WO1998002565A1 (en)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2105446B1 (en) 1998-09-23 2013-08-14 ZymoGenetics, Inc. Cytokine receptor zalpha11
WO2000040716A2 (en) 1999-01-07 2000-07-13 Zymogenetics, Inc. Soluble receptor br43x2 and methods of using them for therapy
US7833529B1 (en) 1999-01-07 2010-11-16 Zymogenetics, Inc. Methods for inhibiting B lymphocyte proliferation with soluble ztnf4 receptor
EP1165791B1 (en) 1999-03-09 2007-10-31 ZymoGenetics, Inc. Human cytokine as ligand of the zalpha receptor and uses thereof
AU3910000A (en) * 1999-03-22 2000-10-09 Zymogenetics Inc. Improved methods for producing proteins in transformed (pichia)
US6258559B1 (en) 1999-03-22 2001-07-10 Zymogenetics, Inc. Method for producing proteins in transformed Pichia
IL147336A0 (en) 1999-07-07 2002-08-14 Zymogenetics Inc Human cytokine receptor
CA2395369C (en) 1999-12-23 2014-07-08 Zymogenetics, Inc. Cytokine zcyto18
CA2412239C (en) 2000-06-26 2013-05-28 Zymogenetics, Inc. Cytokine receptor zcytor17
WO2002066516A2 (en) 2001-02-20 2002-08-29 Zymogenetics, Inc. Antibodies that bind both bcma and taci
KR20040030628A (en) 2001-05-24 2004-04-09 지모제넥틱스, 인코포레이티드 Taci-immunoglobulin fusion proteins
CN105131104B (en) 2001-10-10 2018-11-16 诺和诺德公司 The reconstruct and sugar conjugation of peptide
DK1578771T3 (en) 2001-10-10 2013-06-10 Novo Nordisk As Remodeling and glycoconjugation of peptides
US6929932B2 (en) 2001-11-05 2005-08-16 Zymogenetics, Inc. IL-21 antagonists
AU2003280410B8 (en) 2002-01-18 2009-04-23 Zymogenetics, Inc. Cytokine receptor zcytor17 multimers
DK1476541T3 (en) 2002-01-18 2008-11-03 Zymogenetics Inc Cytokine (zcytor17 ligand)
EP1497415B1 (en) 2002-04-19 2010-12-29 ZymoGenetics, L.L.C. Methods for detection or modulation of the interaction of a cytokine receptor with its ligand
EP2338333B1 (en) 2003-04-09 2017-09-06 ratiopharm GmbH Glycopegylation methods and proteins/peptides produced by the methods
EP2166104B1 (en) 2004-08-02 2013-06-12 BASF Plant Science GmbH Method for isolation of transcription termination sequences
AU2006212807A1 (en) 2005-02-08 2006-08-17 Zymogenetics, Inc. Anti-IL-20, anti-IL-22 and anti-IL-22RA antibodies and binding partners and methods of using in inflammation
US20070054360A1 (en) 2005-05-12 2007-03-08 Zeren Gao Compositions and methods for modulating immune responses
EP1922079A2 (en) 2005-08-09 2008-05-21 ZymoGenetics, Inc. Methods for the treatment and prevention of abnormal cell proliferation using taci-fusion molecules
JP2009507777A (en) 2005-08-09 2009-02-26 ザイモジェネティクス，インコーポレイティド Method for treating B cell tumors using TACI-Ig fusion molecules
CA2621623A1 (en) 2005-09-28 2007-04-05 Zymogenetics, Inc. Il-17a and il-17f antagonists and methods of using the same
US8784812B2 (en) 2006-05-15 2014-07-22 Zymogenetics, Inc. Methods for treating autoimmune diseases using a TACI-Ig fusion molecule
US8859230B2 (en) 2007-11-21 2014-10-14 Roskilde Universitet Polypeptides comprising an ice-binding activity
CN101903531B (en) * 2007-12-19 2013-09-18 格利科菲公司 Yeast strains for protein production
WO2009092806A2 (en) 2008-01-25 2009-07-30 Aarhus Universitet Selective exosite inhibition of papp-a activity against igfbp-4
AU2009327189B2 (en) 2008-12-16 2012-09-06 Novartis Ag Yeast display systems
JP2015509005A (en) 2012-02-01 2015-03-26 シンセティック ジェノミクス インコーポレーテッド Materials and methods for the synthesis of nucleic acid molecules that minimize errors
WO2014202089A2 (en) 2013-06-18 2014-12-24 Roskilde Universitet Variants of anti-freeze polypeptides

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4857467A (en) * 1986-07-23 1989-08-15 Phillips Petroleum Company Carbon and energy source markers for transformation of strains of the genes Pichia
EP0862640A2 (en) * 1995-11-09 1998-09-09 ZymoGenetics, Inc. Production of gad65 in methylotrophic yeast

Also Published As

Publication number Publication date
CA2261151C (en) 2003-09-16
WO1998002565A1 (en) 1998-01-22
AU3803997A (en) 1998-02-09
IL128073A0 (en) 1999-11-30
JP2002515746A (en) 2002-05-28
AU718510B2 (en) 2000-04-13

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Legal Events

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EEER Examination request
MKLA Lapsed